Optimization of fully aligned bioactive electrospun fibers for “in vitro” nerve guidance
Abstract Complex architecture of natural tissues such as nerves requires the use of multifunctional scaffolds with peculiar topological and biochemical signals able to address cell behavior towards specific events at the cellular (microscale) and macromolecular (nanoscale) level. In this context, th...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Cirillo, Valentina [verfasserIn] |
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| Format: |
Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2014 |
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| Schlagwörter: |
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| Anmerkung: |
© Springer Science+Business Media New York 2014 |
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| Übergeordnetes Werk: |
Enthalten in: Journal of materials science / Materials in medicine - Springer US, 1990, 25(2014), 10 vom: 16. Apr., Seite 2323-2332 |
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| Übergeordnetes Werk: |
volume:25 ; year:2014 ; number:10 ; day:16 ; month:04 ; pages:2323-2332 |
| Links: |
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| DOI / URN: |
10.1007/s10856-014-5214-4 |
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| Katalog-ID: |
OLC2066826065 |
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| 520 | |a Abstract Complex architecture of natural tissues such as nerves requires the use of multifunctional scaffolds with peculiar topological and biochemical signals able to address cell behavior towards specific events at the cellular (microscale) and macromolecular (nanoscale) level. In this context, the electrospinning technique is useful to generate fiber assemblies having peculiar fiber diameters at the nanoscale and patterned by unidirectional ways, to facilitate neurite extension via contact guidance. Following a bio-mimetic approach, fully aligned polycaprolactone fibers blended with gelatin macromolecules have been fabricated as potential bioactive substrate for nerve regeneration. Morphological and topographic aspects of electrospun fibers assessed by SEM/AFM microscopy supported by image analyses elaboration allow estimating an increase of fully aligned fibers from 5 to 39 % as collector rotating rate increases from 1,000 to 3,000 rpm. We verify that fully alignment of fibers positively influences in vitro response of hMSC and PC-12 cells in neurogenic way. Immunostaining images show that the presence of topological defects, i.e., kinks—due to more frequent fiber crossing—in the case of randomly organized fiber assembly concurs to interfere with proper neurite outgrowth. On the contrary, fully aligned fibers without kinks offer a more efficient contact guidance to direct the orientation of nerve cells along the fibers respect to randomly organized ones, promoting a high elongation of neurites at 7 days and the formation of bipolar extensions. So, this confirms that the topological cue of fully alignment of fibers elicits a favorable environment for nerve regeneration. | ||
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10.1007/s10856-014-5214-4 doi (DE-627)OLC2066826065 (DE-He213)s10856-014-5214-4-p DE-627 ger DE-627 rakwb eng 610 670 VZ Cirillo, Valentina verfasserin aut Optimization of fully aligned bioactive electrospun fibers for “in vitro” nerve guidance 2014 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer Science+Business Media New York 2014 Abstract Complex architecture of natural tissues such as nerves requires the use of multifunctional scaffolds with peculiar topological and biochemical signals able to address cell behavior towards specific events at the cellular (microscale) and macromolecular (nanoscale) level. In this context, the electrospinning technique is useful to generate fiber assemblies having peculiar fiber diameters at the nanoscale and patterned by unidirectional ways, to facilitate neurite extension via contact guidance. Following a bio-mimetic approach, fully aligned polycaprolactone fibers blended with gelatin macromolecules have been fabricated as potential bioactive substrate for nerve regeneration. Morphological and topographic aspects of electrospun fibers assessed by SEM/AFM microscopy supported by image analyses elaboration allow estimating an increase of fully aligned fibers from 5 to 39 % as collector rotating rate increases from 1,000 to 3,000 rpm. We verify that fully alignment of fibers positively influences in vitro response of hMSC and PC-12 cells in neurogenic way. Immunostaining images show that the presence of topological defects, i.e., kinks—due to more frequent fiber crossing—in the case of randomly organized fiber assembly concurs to interfere with proper neurite outgrowth. On the contrary, fully aligned fibers without kinks offer a more efficient contact guidance to direct the orientation of nerve cells along the fibers respect to randomly organized ones, promoting a high elongation of neurites at 7 days and the formation of bipolar extensions. So, this confirms that the topological cue of fully alignment of fibers elicits a favorable environment for nerve regeneration. Neurite Outgrowth Nerve Regeneration Electrospun Fiber Neurites Length Fiber Alignment Guarino, Vincenzo aut Alvarez-Perez, Marco Antonio aut Marrese, Marica aut Ambrosio, Luigi aut Enthalten in Journal of materials science / Materials in medicine Springer US, 1990 25(2014), 10 vom: 16. Apr., Seite 2323-2332 (DE-627)130865028 (DE-600)1031752-1 (DE-576)023107537 0957-4530 nnns volume:25 year:2014 number:10 day:16 month:04 pages:2323-2332 https://doi.org/10.1007/s10856-014-5214-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_32 GBV_ILN_70 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_4012 GBV_ILN_4046 GBV_ILN_4219 GBV_ILN_4305 GBV_ILN_4323 AR 25 2014 10 16 04 2323-2332 |
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10.1007/s10856-014-5214-4 doi (DE-627)OLC2066826065 (DE-He213)s10856-014-5214-4-p DE-627 ger DE-627 rakwb eng 610 670 VZ Cirillo, Valentina verfasserin aut Optimization of fully aligned bioactive electrospun fibers for “in vitro” nerve guidance 2014 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer Science+Business Media New York 2014 Abstract Complex architecture of natural tissues such as nerves requires the use of multifunctional scaffolds with peculiar topological and biochemical signals able to address cell behavior towards specific events at the cellular (microscale) and macromolecular (nanoscale) level. In this context, the electrospinning technique is useful to generate fiber assemblies having peculiar fiber diameters at the nanoscale and patterned by unidirectional ways, to facilitate neurite extension via contact guidance. Following a bio-mimetic approach, fully aligned polycaprolactone fibers blended with gelatin macromolecules have been fabricated as potential bioactive substrate for nerve regeneration. Morphological and topographic aspects of electrospun fibers assessed by SEM/AFM microscopy supported by image analyses elaboration allow estimating an increase of fully aligned fibers from 5 to 39 % as collector rotating rate increases from 1,000 to 3,000 rpm. We verify that fully alignment of fibers positively influences in vitro response of hMSC and PC-12 cells in neurogenic way. Immunostaining images show that the presence of topological defects, i.e., kinks—due to more frequent fiber crossing—in the case of randomly organized fiber assembly concurs to interfere with proper neurite outgrowth. On the contrary, fully aligned fibers without kinks offer a more efficient contact guidance to direct the orientation of nerve cells along the fibers respect to randomly organized ones, promoting a high elongation of neurites at 7 days and the formation of bipolar extensions. So, this confirms that the topological cue of fully alignment of fibers elicits a favorable environment for nerve regeneration. Neurite Outgrowth Nerve Regeneration Electrospun Fiber Neurites Length Fiber Alignment Guarino, Vincenzo aut Alvarez-Perez, Marco Antonio aut Marrese, Marica aut Ambrosio, Luigi aut Enthalten in Journal of materials science / Materials in medicine Springer US, 1990 25(2014), 10 vom: 16. Apr., Seite 2323-2332 (DE-627)130865028 (DE-600)1031752-1 (DE-576)023107537 0957-4530 nnns volume:25 year:2014 number:10 day:16 month:04 pages:2323-2332 https://doi.org/10.1007/s10856-014-5214-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_32 GBV_ILN_70 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_4012 GBV_ILN_4046 GBV_ILN_4219 GBV_ILN_4305 GBV_ILN_4323 AR 25 2014 10 16 04 2323-2332 |
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10.1007/s10856-014-5214-4 doi (DE-627)OLC2066826065 (DE-He213)s10856-014-5214-4-p DE-627 ger DE-627 rakwb eng 610 670 VZ Cirillo, Valentina verfasserin aut Optimization of fully aligned bioactive electrospun fibers for “in vitro” nerve guidance 2014 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer Science+Business Media New York 2014 Abstract Complex architecture of natural tissues such as nerves requires the use of multifunctional scaffolds with peculiar topological and biochemical signals able to address cell behavior towards specific events at the cellular (microscale) and macromolecular (nanoscale) level. In this context, the electrospinning technique is useful to generate fiber assemblies having peculiar fiber diameters at the nanoscale and patterned by unidirectional ways, to facilitate neurite extension via contact guidance. Following a bio-mimetic approach, fully aligned polycaprolactone fibers blended with gelatin macromolecules have been fabricated as potential bioactive substrate for nerve regeneration. Morphological and topographic aspects of electrospun fibers assessed by SEM/AFM microscopy supported by image analyses elaboration allow estimating an increase of fully aligned fibers from 5 to 39 % as collector rotating rate increases from 1,000 to 3,000 rpm. We verify that fully alignment of fibers positively influences in vitro response of hMSC and PC-12 cells in neurogenic way. Immunostaining images show that the presence of topological defects, i.e., kinks—due to more frequent fiber crossing—in the case of randomly organized fiber assembly concurs to interfere with proper neurite outgrowth. On the contrary, fully aligned fibers without kinks offer a more efficient contact guidance to direct the orientation of nerve cells along the fibers respect to randomly organized ones, promoting a high elongation of neurites at 7 days and the formation of bipolar extensions. So, this confirms that the topological cue of fully alignment of fibers elicits a favorable environment for nerve regeneration. Neurite Outgrowth Nerve Regeneration Electrospun Fiber Neurites Length Fiber Alignment Guarino, Vincenzo aut Alvarez-Perez, Marco Antonio aut Marrese, Marica aut Ambrosio, Luigi aut Enthalten in Journal of materials science / Materials in medicine Springer US, 1990 25(2014), 10 vom: 16. Apr., Seite 2323-2332 (DE-627)130865028 (DE-600)1031752-1 (DE-576)023107537 0957-4530 nnns volume:25 year:2014 number:10 day:16 month:04 pages:2323-2332 https://doi.org/10.1007/s10856-014-5214-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_32 GBV_ILN_70 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_4012 GBV_ILN_4046 GBV_ILN_4219 GBV_ILN_4305 GBV_ILN_4323 AR 25 2014 10 16 04 2323-2332 |
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10.1007/s10856-014-5214-4 doi (DE-627)OLC2066826065 (DE-He213)s10856-014-5214-4-p DE-627 ger DE-627 rakwb eng 610 670 VZ Cirillo, Valentina verfasserin aut Optimization of fully aligned bioactive electrospun fibers for “in vitro” nerve guidance 2014 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer Science+Business Media New York 2014 Abstract Complex architecture of natural tissues such as nerves requires the use of multifunctional scaffolds with peculiar topological and biochemical signals able to address cell behavior towards specific events at the cellular (microscale) and macromolecular (nanoscale) level. In this context, the electrospinning technique is useful to generate fiber assemblies having peculiar fiber diameters at the nanoscale and patterned by unidirectional ways, to facilitate neurite extension via contact guidance. Following a bio-mimetic approach, fully aligned polycaprolactone fibers blended with gelatin macromolecules have been fabricated as potential bioactive substrate for nerve regeneration. Morphological and topographic aspects of electrospun fibers assessed by SEM/AFM microscopy supported by image analyses elaboration allow estimating an increase of fully aligned fibers from 5 to 39 % as collector rotating rate increases from 1,000 to 3,000 rpm. We verify that fully alignment of fibers positively influences in vitro response of hMSC and PC-12 cells in neurogenic way. Immunostaining images show that the presence of topological defects, i.e., kinks—due to more frequent fiber crossing—in the case of randomly organized fiber assembly concurs to interfere with proper neurite outgrowth. On the contrary, fully aligned fibers without kinks offer a more efficient contact guidance to direct the orientation of nerve cells along the fibers respect to randomly organized ones, promoting a high elongation of neurites at 7 days and the formation of bipolar extensions. So, this confirms that the topological cue of fully alignment of fibers elicits a favorable environment for nerve regeneration. Neurite Outgrowth Nerve Regeneration Electrospun Fiber Neurites Length Fiber Alignment Guarino, Vincenzo aut Alvarez-Perez, Marco Antonio aut Marrese, Marica aut Ambrosio, Luigi aut Enthalten in Journal of materials science / Materials in medicine Springer US, 1990 25(2014), 10 vom: 16. Apr., Seite 2323-2332 (DE-627)130865028 (DE-600)1031752-1 (DE-576)023107537 0957-4530 nnns volume:25 year:2014 number:10 day:16 month:04 pages:2323-2332 https://doi.org/10.1007/s10856-014-5214-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_32 GBV_ILN_70 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_4012 GBV_ILN_4046 GBV_ILN_4219 GBV_ILN_4305 GBV_ILN_4323 AR 25 2014 10 16 04 2323-2332 |
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10.1007/s10856-014-5214-4 doi (DE-627)OLC2066826065 (DE-He213)s10856-014-5214-4-p DE-627 ger DE-627 rakwb eng 610 670 VZ Cirillo, Valentina verfasserin aut Optimization of fully aligned bioactive electrospun fibers for “in vitro” nerve guidance 2014 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer Science+Business Media New York 2014 Abstract Complex architecture of natural tissues such as nerves requires the use of multifunctional scaffolds with peculiar topological and biochemical signals able to address cell behavior towards specific events at the cellular (microscale) and macromolecular (nanoscale) level. In this context, the electrospinning technique is useful to generate fiber assemblies having peculiar fiber diameters at the nanoscale and patterned by unidirectional ways, to facilitate neurite extension via contact guidance. Following a bio-mimetic approach, fully aligned polycaprolactone fibers blended with gelatin macromolecules have been fabricated as potential bioactive substrate for nerve regeneration. Morphological and topographic aspects of electrospun fibers assessed by SEM/AFM microscopy supported by image analyses elaboration allow estimating an increase of fully aligned fibers from 5 to 39 % as collector rotating rate increases from 1,000 to 3,000 rpm. We verify that fully alignment of fibers positively influences in vitro response of hMSC and PC-12 cells in neurogenic way. Immunostaining images show that the presence of topological defects, i.e., kinks—due to more frequent fiber crossing—in the case of randomly organized fiber assembly concurs to interfere with proper neurite outgrowth. On the contrary, fully aligned fibers without kinks offer a more efficient contact guidance to direct the orientation of nerve cells along the fibers respect to randomly organized ones, promoting a high elongation of neurites at 7 days and the formation of bipolar extensions. So, this confirms that the topological cue of fully alignment of fibers elicits a favorable environment for nerve regeneration. Neurite Outgrowth Nerve Regeneration Electrospun Fiber Neurites Length Fiber Alignment Guarino, Vincenzo aut Alvarez-Perez, Marco Antonio aut Marrese, Marica aut Ambrosio, Luigi aut Enthalten in Journal of materials science / Materials in medicine Springer US, 1990 25(2014), 10 vom: 16. Apr., Seite 2323-2332 (DE-627)130865028 (DE-600)1031752-1 (DE-576)023107537 0957-4530 nnns volume:25 year:2014 number:10 day:16 month:04 pages:2323-2332 https://doi.org/10.1007/s10856-014-5214-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_32 GBV_ILN_70 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_4012 GBV_ILN_4046 GBV_ILN_4219 GBV_ILN_4305 GBV_ILN_4323 AR 25 2014 10 16 04 2323-2332 |
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Cirillo, Valentina Guarino, Vincenzo Alvarez-Perez, Marco Antonio Marrese, Marica Ambrosio, Luigi |
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optimization of fully aligned bioactive electrospun fibers for “in vitro” nerve guidance |
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Optimization of fully aligned bioactive electrospun fibers for “in vitro” nerve guidance |
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Abstract Complex architecture of natural tissues such as nerves requires the use of multifunctional scaffolds with peculiar topological and biochemical signals able to address cell behavior towards specific events at the cellular (microscale) and macromolecular (nanoscale) level. In this context, the electrospinning technique is useful to generate fiber assemblies having peculiar fiber diameters at the nanoscale and patterned by unidirectional ways, to facilitate neurite extension via contact guidance. Following a bio-mimetic approach, fully aligned polycaprolactone fibers blended with gelatin macromolecules have been fabricated as potential bioactive substrate for nerve regeneration. Morphological and topographic aspects of electrospun fibers assessed by SEM/AFM microscopy supported by image analyses elaboration allow estimating an increase of fully aligned fibers from 5 to 39 % as collector rotating rate increases from 1,000 to 3,000 rpm. We verify that fully alignment of fibers positively influences in vitro response of hMSC and PC-12 cells in neurogenic way. Immunostaining images show that the presence of topological defects, i.e., kinks—due to more frequent fiber crossing—in the case of randomly organized fiber assembly concurs to interfere with proper neurite outgrowth. On the contrary, fully aligned fibers without kinks offer a more efficient contact guidance to direct the orientation of nerve cells along the fibers respect to randomly organized ones, promoting a high elongation of neurites at 7 days and the formation of bipolar extensions. So, this confirms that the topological cue of fully alignment of fibers elicits a favorable environment for nerve regeneration. © Springer Science+Business Media New York 2014 |
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Abstract Complex architecture of natural tissues such as nerves requires the use of multifunctional scaffolds with peculiar topological and biochemical signals able to address cell behavior towards specific events at the cellular (microscale) and macromolecular (nanoscale) level. In this context, the electrospinning technique is useful to generate fiber assemblies having peculiar fiber diameters at the nanoscale and patterned by unidirectional ways, to facilitate neurite extension via contact guidance. Following a bio-mimetic approach, fully aligned polycaprolactone fibers blended with gelatin macromolecules have been fabricated as potential bioactive substrate for nerve regeneration. Morphological and topographic aspects of electrospun fibers assessed by SEM/AFM microscopy supported by image analyses elaboration allow estimating an increase of fully aligned fibers from 5 to 39 % as collector rotating rate increases from 1,000 to 3,000 rpm. We verify that fully alignment of fibers positively influences in vitro response of hMSC and PC-12 cells in neurogenic way. Immunostaining images show that the presence of topological defects, i.e., kinks—due to more frequent fiber crossing—in the case of randomly organized fiber assembly concurs to interfere with proper neurite outgrowth. On the contrary, fully aligned fibers without kinks offer a more efficient contact guidance to direct the orientation of nerve cells along the fibers respect to randomly organized ones, promoting a high elongation of neurites at 7 days and the formation of bipolar extensions. So, this confirms that the topological cue of fully alignment of fibers elicits a favorable environment for nerve regeneration. © Springer Science+Business Media New York 2014 |
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Abstract Complex architecture of natural tissues such as nerves requires the use of multifunctional scaffolds with peculiar topological and biochemical signals able to address cell behavior towards specific events at the cellular (microscale) and macromolecular (nanoscale) level. In this context, the electrospinning technique is useful to generate fiber assemblies having peculiar fiber diameters at the nanoscale and patterned by unidirectional ways, to facilitate neurite extension via contact guidance. Following a bio-mimetic approach, fully aligned polycaprolactone fibers blended with gelatin macromolecules have been fabricated as potential bioactive substrate for nerve regeneration. Morphological and topographic aspects of electrospun fibers assessed by SEM/AFM microscopy supported by image analyses elaboration allow estimating an increase of fully aligned fibers from 5 to 39 % as collector rotating rate increases from 1,000 to 3,000 rpm. We verify that fully alignment of fibers positively influences in vitro response of hMSC and PC-12 cells in neurogenic way. Immunostaining images show that the presence of topological defects, i.e., kinks—due to more frequent fiber crossing—in the case of randomly organized fiber assembly concurs to interfere with proper neurite outgrowth. On the contrary, fully aligned fibers without kinks offer a more efficient contact guidance to direct the orientation of nerve cells along the fibers respect to randomly organized ones, promoting a high elongation of neurites at 7 days and the formation of bipolar extensions. So, this confirms that the topological cue of fully alignment of fibers elicits a favorable environment for nerve regeneration. © Springer Science+Business Media New York 2014 |
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