Optimization of Poly(dl-Lactic Acid) Degradation and Evaluation of Biological Re-polymerization
Abstract Poly(dl-lactic acid) or PLA is a biodegradable polymer. It has received much attention since it plays an important role in resolving the global warming problem. The protease produced by Actinomadura keratinilytica strain T16-1 was previously reported as having PLA depolymerase potential and...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Youngpreda, Anupan [verfasserIn] |
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| Format: |
Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2016 |
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| Schlagwörter: |
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| Anmerkung: |
© Springer Science+Business Media New York 2016 |
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| Übergeordnetes Werk: |
Enthalten in: Journal of polymers and the environment - Springer US, 2000, 25(2016), 4 vom: 14. Nov., Seite 1131-1139 |
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| Übergeordnetes Werk: |
volume:25 ; year:2016 ; number:4 ; day:14 ; month:11 ; pages:1131-1139 |
| Links: |
|---|
| DOI / URN: |
10.1007/s10924-016-0885-1 |
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| Katalog-ID: |
OLC2067002287 |
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| 520 | |a Abstract Poly(dl-lactic acid) or PLA is a biodegradable polymer. It has received much attention since it plays an important role in resolving the global warming problem. The protease produced by Actinomadura keratinilytica strain T16-1 was previously reported as having PLA depolymerase potential and being applicable to PLA biodegradation, which was used in this work. Therefore, this research demonstrates the important basic knowledge on the biological degradation process by the crude PLA-degrading enzyme from strain T16-1. Its re-polymerization was evaluated. The optimization of PLA degradation by statistical methods based on central composite design was determined. Approximately 6700 mg/l PLA powder was degraded by the crude enzyme under optimized conditions: an initial enzyme activity of 200 U/ml, incubated at 60 °C for 24 h released 6843 mg/l lactic acid with 82% conversion, which was similar to the commercial enzyme proteinase K (81%). The degradable products were re-polymerized repeatedly by using commercial lipase as a catalyst under a nitrogen atmosphere for 6 h. A PLA oligomer was achieved with a molecular weight of 378 Da (n = 5). This is the first report to demonstrate the high efficiency of the enzyme to degrade 100% of PLA powder and to show the biological recycling process of PLA, which is promising for the treatment and utilization of biodegradable plastic wastes in the future. | ||
| 650 | 4 | |a Poly( | |
| 650 | 4 | |a -lactic acid) | |
| 650 | 4 | |a Biodegradation | |
| 650 | 4 | |a Re-polymerization | |
| 650 | 4 | |a Protease | |
| 700 | 1 | |a Panyachanakul, Titiporn |4 aut | |
| 700 | 1 | |a Kitpreechavanich, Vichien |4 aut | |
| 700 | 1 | |a Sirisansaneeyakul, Sarote |4 aut | |
| 700 | 1 | |a Suksamrarn, Sunit |4 aut | |
| 700 | 1 | |a Tokuyama, Shinji |4 aut | |
| 700 | 1 | |a Krajangsang, Sukhumaporn |4 aut | |
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10.1007/s10924-016-0885-1 doi (DE-627)OLC2067002287 (DE-He213)s10924-016-0885-1-p DE-627 ger DE-627 rakwb eng 660 VZ 58.53$jAbfallwirtschaft bkl 58.52$jTechnischer Bodenschutz$jtechnischer Gewässerschutz bkl 43.50$jUmweltbelastungen bkl Youngpreda, Anupan verfasserin aut Optimization of Poly(dl-Lactic Acid) Degradation and Evaluation of Biological Re-polymerization 2016 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer Science+Business Media New York 2016 Abstract Poly(dl-lactic acid) or PLA is a biodegradable polymer. It has received much attention since it plays an important role in resolving the global warming problem. The protease produced by Actinomadura keratinilytica strain T16-1 was previously reported as having PLA depolymerase potential and being applicable to PLA biodegradation, which was used in this work. Therefore, this research demonstrates the important basic knowledge on the biological degradation process by the crude PLA-degrading enzyme from strain T16-1. Its re-polymerization was evaluated. The optimization of PLA degradation by statistical methods based on central composite design was determined. Approximately 6700 mg/l PLA powder was degraded by the crude enzyme under optimized conditions: an initial enzyme activity of 200 U/ml, incubated at 60 °C for 24 h released 6843 mg/l lactic acid with 82% conversion, which was similar to the commercial enzyme proteinase K (81%). The degradable products were re-polymerized repeatedly by using commercial lipase as a catalyst under a nitrogen atmosphere for 6 h. A PLA oligomer was achieved with a molecular weight of 378 Da (n = 5). This is the first report to demonstrate the high efficiency of the enzyme to degrade 100% of PLA powder and to show the biological recycling process of PLA, which is promising for the treatment and utilization of biodegradable plastic wastes in the future. Poly( -lactic acid) Biodegradation Re-polymerization Protease Panyachanakul, Titiporn aut Kitpreechavanich, Vichien aut Sirisansaneeyakul, Sarote aut Suksamrarn, Sunit aut Tokuyama, Shinji aut Krajangsang, Sukhumaporn aut Enthalten in Journal of polymers and the environment Springer US, 2000 25(2016), 4 vom: 14. Nov., Seite 1131-1139 (DE-627)325700176 (DE-600)2039366-0 (DE-576)9325700174 1566-2543 nnns volume:25 year:2016 number:4 day:14 month:11 pages:1131-1139 https://doi.org/10.1007/s10924-016-0885-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_70 GBV_ILN_4012 58.53$jAbfallwirtschaft VZ 106418831 (DE-625)106418831 58.52$jTechnischer Bodenschutz$jtechnischer Gewässerschutz VZ 106420747 (DE-625)106420747 43.50$jUmweltbelastungen VZ 106416782 (DE-625)106416782 AR 25 2016 4 14 11 1131-1139 |
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optimization of poly(dl-lactic acid) degradation and evaluation of biological re-polymerization |
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Optimization of Poly(dl-Lactic Acid) Degradation and Evaluation of Biological Re-polymerization |
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Abstract Poly(dl-lactic acid) or PLA is a biodegradable polymer. It has received much attention since it plays an important role in resolving the global warming problem. The protease produced by Actinomadura keratinilytica strain T16-1 was previously reported as having PLA depolymerase potential and being applicable to PLA biodegradation, which was used in this work. Therefore, this research demonstrates the important basic knowledge on the biological degradation process by the crude PLA-degrading enzyme from strain T16-1. Its re-polymerization was evaluated. The optimization of PLA degradation by statistical methods based on central composite design was determined. Approximately 6700 mg/l PLA powder was degraded by the crude enzyme under optimized conditions: an initial enzyme activity of 200 U/ml, incubated at 60 °C for 24 h released 6843 mg/l lactic acid with 82% conversion, which was similar to the commercial enzyme proteinase K (81%). The degradable products were re-polymerized repeatedly by using commercial lipase as a catalyst under a nitrogen atmosphere for 6 h. A PLA oligomer was achieved with a molecular weight of 378 Da (n = 5). This is the first report to demonstrate the high efficiency of the enzyme to degrade 100% of PLA powder and to show the biological recycling process of PLA, which is promising for the treatment and utilization of biodegradable plastic wastes in the future. © Springer Science+Business Media New York 2016 |
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Abstract Poly(dl-lactic acid) or PLA is a biodegradable polymer. It has received much attention since it plays an important role in resolving the global warming problem. The protease produced by Actinomadura keratinilytica strain T16-1 was previously reported as having PLA depolymerase potential and being applicable to PLA biodegradation, which was used in this work. Therefore, this research demonstrates the important basic knowledge on the biological degradation process by the crude PLA-degrading enzyme from strain T16-1. Its re-polymerization was evaluated. The optimization of PLA degradation by statistical methods based on central composite design was determined. Approximately 6700 mg/l PLA powder was degraded by the crude enzyme under optimized conditions: an initial enzyme activity of 200 U/ml, incubated at 60 °C for 24 h released 6843 mg/l lactic acid with 82% conversion, which was similar to the commercial enzyme proteinase K (81%). The degradable products were re-polymerized repeatedly by using commercial lipase as a catalyst under a nitrogen atmosphere for 6 h. A PLA oligomer was achieved with a molecular weight of 378 Da (n = 5). This is the first report to demonstrate the high efficiency of the enzyme to degrade 100% of PLA powder and to show the biological recycling process of PLA, which is promising for the treatment and utilization of biodegradable plastic wastes in the future. © Springer Science+Business Media New York 2016 |
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Abstract Poly(dl-lactic acid) or PLA is a biodegradable polymer. It has received much attention since it plays an important role in resolving the global warming problem. The protease produced by Actinomadura keratinilytica strain T16-1 was previously reported as having PLA depolymerase potential and being applicable to PLA biodegradation, which was used in this work. Therefore, this research demonstrates the important basic knowledge on the biological degradation process by the crude PLA-degrading enzyme from strain T16-1. Its re-polymerization was evaluated. The optimization of PLA degradation by statistical methods based on central composite design was determined. Approximately 6700 mg/l PLA powder was degraded by the crude enzyme under optimized conditions: an initial enzyme activity of 200 U/ml, incubated at 60 °C for 24 h released 6843 mg/l lactic acid with 82% conversion, which was similar to the commercial enzyme proteinase K (81%). The degradable products were re-polymerized repeatedly by using commercial lipase as a catalyst under a nitrogen atmosphere for 6 h. A PLA oligomer was achieved with a molecular weight of 378 Da (n = 5). This is the first report to demonstrate the high efficiency of the enzyme to degrade 100% of PLA powder and to show the biological recycling process of PLA, which is promising for the treatment and utilization of biodegradable plastic wastes in the future. © Springer Science+Business Media New York 2016 |
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