Evaluation of Rhizopus oryzae Lipase for the Determination of Regiodistribution in Triacylglycerols with Medium Chain Fatty Acids
Abstract The nutritional profile and rheological behaviors of lipids is both due to fatty acid composition and regiodistribution on external and internal positions of triacylglycerol. Actual methods for regiodistribution analysis having some restrictions, there is still a need for investigating a sa...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Pérignon, Marlène [verfasserIn] |
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| Format: |
Artikel |
|---|---|
| Sprache: |
Englisch |
| Erschienen: |
2011 |
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| Schlagwörter: |
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| Anmerkung: |
© AOCS 2011 |
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| Übergeordnetes Werk: |
Enthalten in: Journal of the American Oil Chemists' Society - Springer-Verlag, 1947, 89(2011), 1 vom: 24. Juni, Seite 89-96 |
|---|---|
| Übergeordnetes Werk: |
volume:89 ; year:2011 ; number:1 ; day:24 ; month:06 ; pages:89-96 |
| Links: |
|---|
| DOI / URN: |
10.1007/s11746-011-1884-y |
|---|
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OLC2068156431 |
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| 520 | |a Abstract The nutritional profile and rheological behaviors of lipids is both due to fatty acid composition and regiodistribution on external and internal positions of triacylglycerol. Actual methods for regiodistribution analysis having some restrictions, there is still a need for investigating a safe, simple and environmentally friendly method for the sn-2 position analysis that could especially be used for the analysis of fats containing medium and short chain fatty acids. The objective of this study was to evaluate the 1,3-selectivity and typoselectivity of Rhizopus oryzae lipase in the presence of short/medium chain fatty acids in partial hydrolysis conditions used for regiodistribution analysis. Structured triacylglycerols containing eight-carbon-chain length fatty acids in the sn-2 position were chemically synthesized using DCC/DMAP coupling agent and purification steps by flash-chromatography. The final product showed very high purity and was used as the substrate for 1,3-selectivity evaluation. Typoselectivity was assessed by investigating partial hydrolysis of equimolar blends of homogeneous TAG. This study confirmed the 1,3-selectivity of Rhizopus oryzae lipase in the hydrolysis conditions used, and revealed that this lipase was less influenced by fatty acids chain length than pancreatic lipase. Considering this, Rhizopus oryzae lipase appeared to be a good candidate for regiodistribution analysis of fats containing medium and short chain fatty acids. | ||
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10.1007/s11746-011-1884-y doi (DE-627)OLC2068156431 (DE-He213)s11746-011-1884-y-p DE-627 ger DE-627 rakwb eng 660 VZ Pérignon, Marlène verfasserin aut Evaluation of Rhizopus oryzae Lipase for the Determination of Regiodistribution in Triacylglycerols with Medium Chain Fatty Acids 2011 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © AOCS 2011 Abstract The nutritional profile and rheological behaviors of lipids is both due to fatty acid composition and regiodistribution on external and internal positions of triacylglycerol. Actual methods for regiodistribution analysis having some restrictions, there is still a need for investigating a safe, simple and environmentally friendly method for the sn-2 position analysis that could especially be used for the analysis of fats containing medium and short chain fatty acids. The objective of this study was to evaluate the 1,3-selectivity and typoselectivity of Rhizopus oryzae lipase in the presence of short/medium chain fatty acids in partial hydrolysis conditions used for regiodistribution analysis. Structured triacylglycerols containing eight-carbon-chain length fatty acids in the sn-2 position were chemically synthesized using DCC/DMAP coupling agent and purification steps by flash-chromatography. The final product showed very high purity and was used as the substrate for 1,3-selectivity evaluation. Typoselectivity was assessed by investigating partial hydrolysis of equimolar blends of homogeneous TAG. This study confirmed the 1,3-selectivity of Rhizopus oryzae lipase in the hydrolysis conditions used, and revealed that this lipase was less influenced by fatty acids chain length than pancreatic lipase. Considering this, Rhizopus oryzae lipase appeared to be a good candidate for regiodistribution analysis of fats containing medium and short chain fatty acids. Structured triacylglycerols Regiodistribution lipase Typoselectivity Lecomte, Jérôme aut Pina, Michel aut Renault, Anne aut Simonneau-Deve, Camille aut Villeneuve, Pierre aut Enthalten in Journal of the American Oil Chemists' Society Springer-Verlag, 1947 89(2011), 1 vom: 24. Juni, Seite 89-96 (DE-627)129595691 (DE-600)240684-6 (DE-576)015088715 0003-021X nnns volume:89 year:2011 number:1 day:24 month:06 pages:89-96 https://doi.org/10.1007/s11746-011-1884-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_70 GBV_ILN_183 GBV_ILN_2018 GBV_ILN_4012 GBV_ILN_4219 GBV_ILN_4317 AR 89 2011 1 24 06 89-96 |
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10.1007/s11746-011-1884-y doi (DE-627)OLC2068156431 (DE-He213)s11746-011-1884-y-p DE-627 ger DE-627 rakwb eng 660 VZ Pérignon, Marlène verfasserin aut Evaluation of Rhizopus oryzae Lipase for the Determination of Regiodistribution in Triacylglycerols with Medium Chain Fatty Acids 2011 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © AOCS 2011 Abstract The nutritional profile and rheological behaviors of lipids is both due to fatty acid composition and regiodistribution on external and internal positions of triacylglycerol. Actual methods for regiodistribution analysis having some restrictions, there is still a need for investigating a safe, simple and environmentally friendly method for the sn-2 position analysis that could especially be used for the analysis of fats containing medium and short chain fatty acids. The objective of this study was to evaluate the 1,3-selectivity and typoselectivity of Rhizopus oryzae lipase in the presence of short/medium chain fatty acids in partial hydrolysis conditions used for regiodistribution analysis. Structured triacylglycerols containing eight-carbon-chain length fatty acids in the sn-2 position were chemically synthesized using DCC/DMAP coupling agent and purification steps by flash-chromatography. The final product showed very high purity and was used as the substrate for 1,3-selectivity evaluation. Typoselectivity was assessed by investigating partial hydrolysis of equimolar blends of homogeneous TAG. This study confirmed the 1,3-selectivity of Rhizopus oryzae lipase in the hydrolysis conditions used, and revealed that this lipase was less influenced by fatty acids chain length than pancreatic lipase. Considering this, Rhizopus oryzae lipase appeared to be a good candidate for regiodistribution analysis of fats containing medium and short chain fatty acids. Structured triacylglycerols Regiodistribution lipase Typoselectivity Lecomte, Jérôme aut Pina, Michel aut Renault, Anne aut Simonneau-Deve, Camille aut Villeneuve, Pierre aut Enthalten in Journal of the American Oil Chemists' Society Springer-Verlag, 1947 89(2011), 1 vom: 24. Juni, Seite 89-96 (DE-627)129595691 (DE-600)240684-6 (DE-576)015088715 0003-021X nnns volume:89 year:2011 number:1 day:24 month:06 pages:89-96 https://doi.org/10.1007/s11746-011-1884-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_70 GBV_ILN_183 GBV_ILN_2018 GBV_ILN_4012 GBV_ILN_4219 GBV_ILN_4317 AR 89 2011 1 24 06 89-96 |
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10.1007/s11746-011-1884-y doi (DE-627)OLC2068156431 (DE-He213)s11746-011-1884-y-p DE-627 ger DE-627 rakwb eng 660 VZ Pérignon, Marlène verfasserin aut Evaluation of Rhizopus oryzae Lipase for the Determination of Regiodistribution in Triacylglycerols with Medium Chain Fatty Acids 2011 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © AOCS 2011 Abstract The nutritional profile and rheological behaviors of lipids is both due to fatty acid composition and regiodistribution on external and internal positions of triacylglycerol. Actual methods for regiodistribution analysis having some restrictions, there is still a need for investigating a safe, simple and environmentally friendly method for the sn-2 position analysis that could especially be used for the analysis of fats containing medium and short chain fatty acids. The objective of this study was to evaluate the 1,3-selectivity and typoselectivity of Rhizopus oryzae lipase in the presence of short/medium chain fatty acids in partial hydrolysis conditions used for regiodistribution analysis. Structured triacylglycerols containing eight-carbon-chain length fatty acids in the sn-2 position were chemically synthesized using DCC/DMAP coupling agent and purification steps by flash-chromatography. The final product showed very high purity and was used as the substrate for 1,3-selectivity evaluation. Typoselectivity was assessed by investigating partial hydrolysis of equimolar blends of homogeneous TAG. This study confirmed the 1,3-selectivity of Rhizopus oryzae lipase in the hydrolysis conditions used, and revealed that this lipase was less influenced by fatty acids chain length than pancreatic lipase. Considering this, Rhizopus oryzae lipase appeared to be a good candidate for regiodistribution analysis of fats containing medium and short chain fatty acids. Structured triacylglycerols Regiodistribution lipase Typoselectivity Lecomte, Jérôme aut Pina, Michel aut Renault, Anne aut Simonneau-Deve, Camille aut Villeneuve, Pierre aut Enthalten in Journal of the American Oil Chemists' Society Springer-Verlag, 1947 89(2011), 1 vom: 24. Juni, Seite 89-96 (DE-627)129595691 (DE-600)240684-6 (DE-576)015088715 0003-021X nnns volume:89 year:2011 number:1 day:24 month:06 pages:89-96 https://doi.org/10.1007/s11746-011-1884-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_70 GBV_ILN_183 GBV_ILN_2018 GBV_ILN_4012 GBV_ILN_4219 GBV_ILN_4317 AR 89 2011 1 24 06 89-96 |
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10.1007/s11746-011-1884-y doi (DE-627)OLC2068156431 (DE-He213)s11746-011-1884-y-p DE-627 ger DE-627 rakwb eng 660 VZ Pérignon, Marlène verfasserin aut Evaluation of Rhizopus oryzae Lipase for the Determination of Regiodistribution in Triacylglycerols with Medium Chain Fatty Acids 2011 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © AOCS 2011 Abstract The nutritional profile and rheological behaviors of lipids is both due to fatty acid composition and regiodistribution on external and internal positions of triacylglycerol. Actual methods for regiodistribution analysis having some restrictions, there is still a need for investigating a safe, simple and environmentally friendly method for the sn-2 position analysis that could especially be used for the analysis of fats containing medium and short chain fatty acids. The objective of this study was to evaluate the 1,3-selectivity and typoselectivity of Rhizopus oryzae lipase in the presence of short/medium chain fatty acids in partial hydrolysis conditions used for regiodistribution analysis. Structured triacylglycerols containing eight-carbon-chain length fatty acids in the sn-2 position were chemically synthesized using DCC/DMAP coupling agent and purification steps by flash-chromatography. The final product showed very high purity and was used as the substrate for 1,3-selectivity evaluation. Typoselectivity was assessed by investigating partial hydrolysis of equimolar blends of homogeneous TAG. This study confirmed the 1,3-selectivity of Rhizopus oryzae lipase in the hydrolysis conditions used, and revealed that this lipase was less influenced by fatty acids chain length than pancreatic lipase. Considering this, Rhizopus oryzae lipase appeared to be a good candidate for regiodistribution analysis of fats containing medium and short chain fatty acids. Structured triacylglycerols Regiodistribution lipase Typoselectivity Lecomte, Jérôme aut Pina, Michel aut Renault, Anne aut Simonneau-Deve, Camille aut Villeneuve, Pierre aut Enthalten in Journal of the American Oil Chemists' Society Springer-Verlag, 1947 89(2011), 1 vom: 24. Juni, Seite 89-96 (DE-627)129595691 (DE-600)240684-6 (DE-576)015088715 0003-021X nnns volume:89 year:2011 number:1 day:24 month:06 pages:89-96 https://doi.org/10.1007/s11746-011-1884-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_70 GBV_ILN_183 GBV_ILN_2018 GBV_ILN_4012 GBV_ILN_4219 GBV_ILN_4317 AR 89 2011 1 24 06 89-96 |
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10.1007/s11746-011-1884-y doi (DE-627)OLC2068156431 (DE-He213)s11746-011-1884-y-p DE-627 ger DE-627 rakwb eng 660 VZ Pérignon, Marlène verfasserin aut Evaluation of Rhizopus oryzae Lipase for the Determination of Regiodistribution in Triacylglycerols with Medium Chain Fatty Acids 2011 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © AOCS 2011 Abstract The nutritional profile and rheological behaviors of lipids is both due to fatty acid composition and regiodistribution on external and internal positions of triacylglycerol. Actual methods for regiodistribution analysis having some restrictions, there is still a need for investigating a safe, simple and environmentally friendly method for the sn-2 position analysis that could especially be used for the analysis of fats containing medium and short chain fatty acids. The objective of this study was to evaluate the 1,3-selectivity and typoselectivity of Rhizopus oryzae lipase in the presence of short/medium chain fatty acids in partial hydrolysis conditions used for regiodistribution analysis. Structured triacylglycerols containing eight-carbon-chain length fatty acids in the sn-2 position were chemically synthesized using DCC/DMAP coupling agent and purification steps by flash-chromatography. The final product showed very high purity and was used as the substrate for 1,3-selectivity evaluation. Typoselectivity was assessed by investigating partial hydrolysis of equimolar blends of homogeneous TAG. This study confirmed the 1,3-selectivity of Rhizopus oryzae lipase in the hydrolysis conditions used, and revealed that this lipase was less influenced by fatty acids chain length than pancreatic lipase. Considering this, Rhizopus oryzae lipase appeared to be a good candidate for regiodistribution analysis of fats containing medium and short chain fatty acids. Structured triacylglycerols Regiodistribution lipase Typoselectivity Lecomte, Jérôme aut Pina, Michel aut Renault, Anne aut Simonneau-Deve, Camille aut Villeneuve, Pierre aut Enthalten in Journal of the American Oil Chemists' Society Springer-Verlag, 1947 89(2011), 1 vom: 24. Juni, Seite 89-96 (DE-627)129595691 (DE-600)240684-6 (DE-576)015088715 0003-021X nnns volume:89 year:2011 number:1 day:24 month:06 pages:89-96 https://doi.org/10.1007/s11746-011-1884-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_70 GBV_ILN_183 GBV_ILN_2018 GBV_ILN_4012 GBV_ILN_4219 GBV_ILN_4317 AR 89 2011 1 24 06 89-96 |
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660 VZ Evaluation of Rhizopus oryzae Lipase for the Determination of Regiodistribution in Triacylglycerols with Medium Chain Fatty Acids Structured triacylglycerols Regiodistribution lipase Typoselectivity |
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ddc 660 misc Structured triacylglycerols misc Regiodistribution misc lipase misc Typoselectivity |
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Evaluation of Rhizopus oryzae Lipase for the Determination of Regiodistribution in Triacylglycerols with Medium Chain Fatty Acids |
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Evaluation of Rhizopus oryzae Lipase for the Determination of Regiodistribution in Triacylglycerols with Medium Chain Fatty Acids |
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Pérignon, Marlène |
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Journal of the American Oil Chemists' Society |
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Pérignon, Marlène Lecomte, Jérôme Pina, Michel Renault, Anne Simonneau-Deve, Camille Villeneuve, Pierre |
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evaluation of rhizopus oryzae lipase for the determination of regiodistribution in triacylglycerols with medium chain fatty acids |
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Evaluation of Rhizopus oryzae Lipase for the Determination of Regiodistribution in Triacylglycerols with Medium Chain Fatty Acids |
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Abstract The nutritional profile and rheological behaviors of lipids is both due to fatty acid composition and regiodistribution on external and internal positions of triacylglycerol. Actual methods for regiodistribution analysis having some restrictions, there is still a need for investigating a safe, simple and environmentally friendly method for the sn-2 position analysis that could especially be used for the analysis of fats containing medium and short chain fatty acids. The objective of this study was to evaluate the 1,3-selectivity and typoselectivity of Rhizopus oryzae lipase in the presence of short/medium chain fatty acids in partial hydrolysis conditions used for regiodistribution analysis. Structured triacylglycerols containing eight-carbon-chain length fatty acids in the sn-2 position were chemically synthesized using DCC/DMAP coupling agent and purification steps by flash-chromatography. The final product showed very high purity and was used as the substrate for 1,3-selectivity evaluation. Typoselectivity was assessed by investigating partial hydrolysis of equimolar blends of homogeneous TAG. This study confirmed the 1,3-selectivity of Rhizopus oryzae lipase in the hydrolysis conditions used, and revealed that this lipase was less influenced by fatty acids chain length than pancreatic lipase. Considering this, Rhizopus oryzae lipase appeared to be a good candidate for regiodistribution analysis of fats containing medium and short chain fatty acids. © AOCS 2011 |
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Abstract The nutritional profile and rheological behaviors of lipids is both due to fatty acid composition and regiodistribution on external and internal positions of triacylglycerol. Actual methods for regiodistribution analysis having some restrictions, there is still a need for investigating a safe, simple and environmentally friendly method for the sn-2 position analysis that could especially be used for the analysis of fats containing medium and short chain fatty acids. The objective of this study was to evaluate the 1,3-selectivity and typoselectivity of Rhizopus oryzae lipase in the presence of short/medium chain fatty acids in partial hydrolysis conditions used for regiodistribution analysis. Structured triacylglycerols containing eight-carbon-chain length fatty acids in the sn-2 position were chemically synthesized using DCC/DMAP coupling agent and purification steps by flash-chromatography. The final product showed very high purity and was used as the substrate for 1,3-selectivity evaluation. Typoselectivity was assessed by investigating partial hydrolysis of equimolar blends of homogeneous TAG. This study confirmed the 1,3-selectivity of Rhizopus oryzae lipase in the hydrolysis conditions used, and revealed that this lipase was less influenced by fatty acids chain length than pancreatic lipase. Considering this, Rhizopus oryzae lipase appeared to be a good candidate for regiodistribution analysis of fats containing medium and short chain fatty acids. © AOCS 2011 |
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Abstract The nutritional profile and rheological behaviors of lipids is both due to fatty acid composition and regiodistribution on external and internal positions of triacylglycerol. Actual methods for regiodistribution analysis having some restrictions, there is still a need for investigating a safe, simple and environmentally friendly method for the sn-2 position analysis that could especially be used for the analysis of fats containing medium and short chain fatty acids. The objective of this study was to evaluate the 1,3-selectivity and typoselectivity of Rhizopus oryzae lipase in the presence of short/medium chain fatty acids in partial hydrolysis conditions used for regiodistribution analysis. Structured triacylglycerols containing eight-carbon-chain length fatty acids in the sn-2 position were chemically synthesized using DCC/DMAP coupling agent and purification steps by flash-chromatography. The final product showed very high purity and was used as the substrate for 1,3-selectivity evaluation. Typoselectivity was assessed by investigating partial hydrolysis of equimolar blends of homogeneous TAG. This study confirmed the 1,3-selectivity of Rhizopus oryzae lipase in the hydrolysis conditions used, and revealed that this lipase was less influenced by fatty acids chain length than pancreatic lipase. Considering this, Rhizopus oryzae lipase appeared to be a good candidate for regiodistribution analysis of fats containing medium and short chain fatty acids. © AOCS 2011 |
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Evaluation of Rhizopus oryzae Lipase for the Determination of Regiodistribution in Triacylglycerols with Medium Chain Fatty Acids |
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