Microstructured Arrays with Pre-synthesized Capture Probes for DNA Detection Based on Metal Nanoparticles and Silver Enhancement
Abstract DNA microarrays exhibiting highly defined squared spot geometry and increased homogeneity in capture DNA distribution were prepared by a combination of microfabrication and DNA spotting. The microfabricated substrates were tested using metal nanoparticles as markers. The optical absorption...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Zhang, Guo-Jun [verfasserIn] |
|---|
| Format: |
Artikel |
|---|---|
| Sprache: |
Englisch |
| Erschienen: |
2004 |
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| Anmerkung: |
© Plenum Publishing Corporation 2004 |
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| Übergeordnetes Werk: |
Enthalten in: Journal of fluorescence - Kluwer Academic Publishers-Plenum Publishers, 1991, 14(2004), 4 vom: Juli, Seite 369-375 |
|---|---|
| Übergeordnetes Werk: |
volume:14 ; year:2004 ; number:4 ; month:07 ; pages:369-375 |
| Links: |
|---|
| DOI / URN: |
10.1023/B:JOFL.0000031818.39925.4c |
|---|
| Katalog-ID: |
OLC2070040917 |
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| 520 | |a Abstract DNA microarrays exhibiting highly defined squared spot geometry and increased homogeneity in capture DNA distribution were prepared by a combination of microfabrication and DNA spotting. The microfabricated substrates were tested using metal nanoparticles as markers. The optical absorption signal is improved by a silver enhancement step. Experiments demonstrated the specificity and the concentration-dependency of the signal. | ||
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Abstract DNA microarrays exhibiting highly defined squared spot geometry and increased homogeneity in capture DNA distribution were prepared by a combination of microfabrication and DNA spotting. The microfabricated substrates were tested using metal nanoparticles as markers. The optical absorption signal is improved by a silver enhancement step. Experiments demonstrated the specificity and the concentration-dependency of the signal. © Plenum Publishing Corporation 2004 |
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Abstract DNA microarrays exhibiting highly defined squared spot geometry and increased homogeneity in capture DNA distribution were prepared by a combination of microfabrication and DNA spotting. The microfabricated substrates were tested using metal nanoparticles as markers. The optical absorption signal is improved by a silver enhancement step. Experiments demonstrated the specificity and the concentration-dependency of the signal. © Plenum Publishing Corporation 2004 |
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