Fluorescence Resonance Energy Transfer and Complex Formation Between Thiazole Orange and Various Dye-DNA Conjugates: Implications in Signaling Nucleic Acid Hybridization
Abstract Fluorescence resonance energy transfer (FRET) was investigated between the intercalating dye thiazole orange (TO), and the dyes Cyanine 3 (Cy3), Cyanine 5 (Cy5), Carboxytetramethyl Rhodamine (TAMRA), Iowa Black FQ (IabFQ), and Iowa Black RQ (IabRQ), which were covalently immobilized at the...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Algar, W. Russ [verfasserIn] |
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| Format: |
Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2006 |
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| Schlagwörter: |
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| Anmerkung: |
© Springer Science+Business Media, Inc. 2006 |
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| Übergeordnetes Werk: |
Enthalten in: Journal of fluorescence - Springer US, 1991, 16(2006), 4 vom: 23. Juni, Seite 555-567 |
|---|---|
| Übergeordnetes Werk: |
volume:16 ; year:2006 ; number:4 ; day:23 ; month:06 ; pages:555-567 |
| Links: |
|---|
| DOI / URN: |
10.1007/s10895-006-0091-y |
|---|
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OLC2070043118 |
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| 520 | |a Abstract Fluorescence resonance energy transfer (FRET) was investigated between the intercalating dye thiazole orange (TO), and the dyes Cyanine 3 (Cy3), Cyanine 5 (Cy5), Carboxytetramethyl Rhodamine (TAMRA), Iowa Black FQ (IabFQ), and Iowa Black RQ (IabRQ), which were covalently immobilized at the end of dsDNA oligonucleotides. In addition to determining that TO was an effective energy donor, FRET efficiency data obtained from fluorescence lifetime measurements indicated that TO intercalated near the middle of the 19mer oligonucleotide sequence that was used in this study. Discrepancies in FRET efficiencies obtained from intensity and lifetime measurements led to the investigation of non-fluorescent complex formation between TAMRA and modified TO. The hydrophobicity of TO was modified by the addition of either an alkyl or polyethylene glycol (PEG) side-chain to study effects of dimer and aggregate formation. It was found that at stoichiometric excesses of modified TO, fluorescence quenching of TAMRA was observed, and that this could be correlated to the hydrophobicity of a TO-chain species. The TAMRA:TO-chain association constant for the TO-alkyl system was 0.043±0.002 $ M^{−1} $, while that obtained for the TO-PEG was 0.037±0.002 $ M^{−1} $. From the perspective of method development for the transduction of hybridization events, we present and evaluate a variety of schemes based on energy transfer between TO and an acceptor dye, and discuss the implications of complex formation in such schemes. | ||
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10.1007/s10895-006-0091-y doi (DE-627)OLC2070043118 (DE-He213)s10895-006-0091-y-p DE-627 ger DE-627 rakwb eng 620 VZ Algar, W. Russ verfasserin aut Fluorescence Resonance Energy Transfer and Complex Formation Between Thiazole Orange and Various Dye-DNA Conjugates: Implications in Signaling Nucleic Acid Hybridization 2006 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer Science+Business Media, Inc. 2006 Abstract Fluorescence resonance energy transfer (FRET) was investigated between the intercalating dye thiazole orange (TO), and the dyes Cyanine 3 (Cy3), Cyanine 5 (Cy5), Carboxytetramethyl Rhodamine (TAMRA), Iowa Black FQ (IabFQ), and Iowa Black RQ (IabRQ), which were covalently immobilized at the end of dsDNA oligonucleotides. In addition to determining that TO was an effective energy donor, FRET efficiency data obtained from fluorescence lifetime measurements indicated that TO intercalated near the middle of the 19mer oligonucleotide sequence that was used in this study. Discrepancies in FRET efficiencies obtained from intensity and lifetime measurements led to the investigation of non-fluorescent complex formation between TAMRA and modified TO. The hydrophobicity of TO was modified by the addition of either an alkyl or polyethylene glycol (PEG) side-chain to study effects of dimer and aggregate formation. It was found that at stoichiometric excesses of modified TO, fluorescence quenching of TAMRA was observed, and that this could be correlated to the hydrophobicity of a TO-chain species. The TAMRA:TO-chain association constant for the TO-alkyl system was 0.043±0.002 $ M^{−1} $, while that obtained for the TO-PEG was 0.037±0.002 $ M^{−1} $. From the perspective of method development for the transduction of hybridization events, we present and evaluate a variety of schemes based on energy transfer between TO and an acceptor dye, and discuss the implications of complex formation in such schemes. Fluorescence resonance energy transfer Thiazole orange Förster distance Biosensor TAMRA Massey, Melissa aut Krull, Ulrich J. aut Enthalten in Journal of fluorescence Springer US, 1991 16(2006), 4 vom: 23. Juni, Seite 555-567 (DE-627)130988731 (DE-600)1079500-5 (DE-576)030293898 1053-0509 nnns volume:16 year:2006 number:4 day:23 month:06 pages:555-567 https://doi.org/10.1007/s10895-006-0091-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_70 GBV_ILN_285 GBV_ILN_2004 GBV_ILN_4125 AR 16 2006 4 23 06 555-567 |
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10.1007/s10895-006-0091-y doi (DE-627)OLC2070043118 (DE-He213)s10895-006-0091-y-p DE-627 ger DE-627 rakwb eng 620 VZ Algar, W. Russ verfasserin aut Fluorescence Resonance Energy Transfer and Complex Formation Between Thiazole Orange and Various Dye-DNA Conjugates: Implications in Signaling Nucleic Acid Hybridization 2006 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer Science+Business Media, Inc. 2006 Abstract Fluorescence resonance energy transfer (FRET) was investigated between the intercalating dye thiazole orange (TO), and the dyes Cyanine 3 (Cy3), Cyanine 5 (Cy5), Carboxytetramethyl Rhodamine (TAMRA), Iowa Black FQ (IabFQ), and Iowa Black RQ (IabRQ), which were covalently immobilized at the end of dsDNA oligonucleotides. In addition to determining that TO was an effective energy donor, FRET efficiency data obtained from fluorescence lifetime measurements indicated that TO intercalated near the middle of the 19mer oligonucleotide sequence that was used in this study. Discrepancies in FRET efficiencies obtained from intensity and lifetime measurements led to the investigation of non-fluorescent complex formation between TAMRA and modified TO. The hydrophobicity of TO was modified by the addition of either an alkyl or polyethylene glycol (PEG) side-chain to study effects of dimer and aggregate formation. It was found that at stoichiometric excesses of modified TO, fluorescence quenching of TAMRA was observed, and that this could be correlated to the hydrophobicity of a TO-chain species. The TAMRA:TO-chain association constant for the TO-alkyl system was 0.043±0.002 $ M^{−1} $, while that obtained for the TO-PEG was 0.037±0.002 $ M^{−1} $. From the perspective of method development for the transduction of hybridization events, we present and evaluate a variety of schemes based on energy transfer between TO and an acceptor dye, and discuss the implications of complex formation in such schemes. Fluorescence resonance energy transfer Thiazole orange Förster distance Biosensor TAMRA Massey, Melissa aut Krull, Ulrich J. aut Enthalten in Journal of fluorescence Springer US, 1991 16(2006), 4 vom: 23. Juni, Seite 555-567 (DE-627)130988731 (DE-600)1079500-5 (DE-576)030293898 1053-0509 nnns volume:16 year:2006 number:4 day:23 month:06 pages:555-567 https://doi.org/10.1007/s10895-006-0091-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_70 GBV_ILN_285 GBV_ILN_2004 GBV_ILN_4125 AR 16 2006 4 23 06 555-567 |
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10.1007/s10895-006-0091-y doi (DE-627)OLC2070043118 (DE-He213)s10895-006-0091-y-p DE-627 ger DE-627 rakwb eng 620 VZ Algar, W. Russ verfasserin aut Fluorescence Resonance Energy Transfer and Complex Formation Between Thiazole Orange and Various Dye-DNA Conjugates: Implications in Signaling Nucleic Acid Hybridization 2006 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer Science+Business Media, Inc. 2006 Abstract Fluorescence resonance energy transfer (FRET) was investigated between the intercalating dye thiazole orange (TO), and the dyes Cyanine 3 (Cy3), Cyanine 5 (Cy5), Carboxytetramethyl Rhodamine (TAMRA), Iowa Black FQ (IabFQ), and Iowa Black RQ (IabRQ), which were covalently immobilized at the end of dsDNA oligonucleotides. In addition to determining that TO was an effective energy donor, FRET efficiency data obtained from fluorescence lifetime measurements indicated that TO intercalated near the middle of the 19mer oligonucleotide sequence that was used in this study. Discrepancies in FRET efficiencies obtained from intensity and lifetime measurements led to the investigation of non-fluorescent complex formation between TAMRA and modified TO. The hydrophobicity of TO was modified by the addition of either an alkyl or polyethylene glycol (PEG) side-chain to study effects of dimer and aggregate formation. It was found that at stoichiometric excesses of modified TO, fluorescence quenching of TAMRA was observed, and that this could be correlated to the hydrophobicity of a TO-chain species. The TAMRA:TO-chain association constant for the TO-alkyl system was 0.043±0.002 $ M^{−1} $, while that obtained for the TO-PEG was 0.037±0.002 $ M^{−1} $. From the perspective of method development for the transduction of hybridization events, we present and evaluate a variety of schemes based on energy transfer between TO and an acceptor dye, and discuss the implications of complex formation in such schemes. Fluorescence resonance energy transfer Thiazole orange Förster distance Biosensor TAMRA Massey, Melissa aut Krull, Ulrich J. aut Enthalten in Journal of fluorescence Springer US, 1991 16(2006), 4 vom: 23. Juni, Seite 555-567 (DE-627)130988731 (DE-600)1079500-5 (DE-576)030293898 1053-0509 nnns volume:16 year:2006 number:4 day:23 month:06 pages:555-567 https://doi.org/10.1007/s10895-006-0091-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_70 GBV_ILN_285 GBV_ILN_2004 GBV_ILN_4125 AR 16 2006 4 23 06 555-567 |
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10.1007/s10895-006-0091-y doi (DE-627)OLC2070043118 (DE-He213)s10895-006-0091-y-p DE-627 ger DE-627 rakwb eng 620 VZ Algar, W. Russ verfasserin aut Fluorescence Resonance Energy Transfer and Complex Formation Between Thiazole Orange and Various Dye-DNA Conjugates: Implications in Signaling Nucleic Acid Hybridization 2006 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer Science+Business Media, Inc. 2006 Abstract Fluorescence resonance energy transfer (FRET) was investigated between the intercalating dye thiazole orange (TO), and the dyes Cyanine 3 (Cy3), Cyanine 5 (Cy5), Carboxytetramethyl Rhodamine (TAMRA), Iowa Black FQ (IabFQ), and Iowa Black RQ (IabRQ), which were covalently immobilized at the end of dsDNA oligonucleotides. In addition to determining that TO was an effective energy donor, FRET efficiency data obtained from fluorescence lifetime measurements indicated that TO intercalated near the middle of the 19mer oligonucleotide sequence that was used in this study. Discrepancies in FRET efficiencies obtained from intensity and lifetime measurements led to the investigation of non-fluorescent complex formation between TAMRA and modified TO. The hydrophobicity of TO was modified by the addition of either an alkyl or polyethylene glycol (PEG) side-chain to study effects of dimer and aggregate formation. It was found that at stoichiometric excesses of modified TO, fluorescence quenching of TAMRA was observed, and that this could be correlated to the hydrophobicity of a TO-chain species. The TAMRA:TO-chain association constant for the TO-alkyl system was 0.043±0.002 $ M^{−1} $, while that obtained for the TO-PEG was 0.037±0.002 $ M^{−1} $. From the perspective of method development for the transduction of hybridization events, we present and evaluate a variety of schemes based on energy transfer between TO and an acceptor dye, and discuss the implications of complex formation in such schemes. Fluorescence resonance energy transfer Thiazole orange Förster distance Biosensor TAMRA Massey, Melissa aut Krull, Ulrich J. aut Enthalten in Journal of fluorescence Springer US, 1991 16(2006), 4 vom: 23. Juni, Seite 555-567 (DE-627)130988731 (DE-600)1079500-5 (DE-576)030293898 1053-0509 nnns volume:16 year:2006 number:4 day:23 month:06 pages:555-567 https://doi.org/10.1007/s10895-006-0091-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_70 GBV_ILN_285 GBV_ILN_2004 GBV_ILN_4125 AR 16 2006 4 23 06 555-567 |
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10.1007/s10895-006-0091-y doi (DE-627)OLC2070043118 (DE-He213)s10895-006-0091-y-p DE-627 ger DE-627 rakwb eng 620 VZ Algar, W. Russ verfasserin aut Fluorescence Resonance Energy Transfer and Complex Formation Between Thiazole Orange and Various Dye-DNA Conjugates: Implications in Signaling Nucleic Acid Hybridization 2006 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer Science+Business Media, Inc. 2006 Abstract Fluorescence resonance energy transfer (FRET) was investigated between the intercalating dye thiazole orange (TO), and the dyes Cyanine 3 (Cy3), Cyanine 5 (Cy5), Carboxytetramethyl Rhodamine (TAMRA), Iowa Black FQ (IabFQ), and Iowa Black RQ (IabRQ), which were covalently immobilized at the end of dsDNA oligonucleotides. In addition to determining that TO was an effective energy donor, FRET efficiency data obtained from fluorescence lifetime measurements indicated that TO intercalated near the middle of the 19mer oligonucleotide sequence that was used in this study. Discrepancies in FRET efficiencies obtained from intensity and lifetime measurements led to the investigation of non-fluorescent complex formation between TAMRA and modified TO. The hydrophobicity of TO was modified by the addition of either an alkyl or polyethylene glycol (PEG) side-chain to study effects of dimer and aggregate formation. It was found that at stoichiometric excesses of modified TO, fluorescence quenching of TAMRA was observed, and that this could be correlated to the hydrophobicity of a TO-chain species. The TAMRA:TO-chain association constant for the TO-alkyl system was 0.043±0.002 $ M^{−1} $, while that obtained for the TO-PEG was 0.037±0.002 $ M^{−1} $. From the perspective of method development for the transduction of hybridization events, we present and evaluate a variety of schemes based on energy transfer between TO and an acceptor dye, and discuss the implications of complex formation in such schemes. Fluorescence resonance energy transfer Thiazole orange Förster distance Biosensor TAMRA Massey, Melissa aut Krull, Ulrich J. aut Enthalten in Journal of fluorescence Springer US, 1991 16(2006), 4 vom: 23. Juni, Seite 555-567 (DE-627)130988731 (DE-600)1079500-5 (DE-576)030293898 1053-0509 nnns volume:16 year:2006 number:4 day:23 month:06 pages:555-567 https://doi.org/10.1007/s10895-006-0091-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_70 GBV_ILN_285 GBV_ILN_2004 GBV_ILN_4125 AR 16 2006 4 23 06 555-567 |
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620 VZ Fluorescence Resonance Energy Transfer and Complex Formation Between Thiazole Orange and Various Dye-DNA Conjugates: Implications in Signaling Nucleic Acid Hybridization Fluorescence resonance energy transfer Thiazole orange Förster distance Biosensor TAMRA |
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Fluorescence Resonance Energy Transfer and Complex Formation Between Thiazole Orange and Various Dye-DNA Conjugates: Implications in Signaling Nucleic Acid Hybridization |
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Fluorescence Resonance Energy Transfer and Complex Formation Between Thiazole Orange and Various Dye-DNA Conjugates: Implications in Signaling Nucleic Acid Hybridization |
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Algar, W. Russ |
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Algar, W. Russ Massey, Melissa Krull, Ulrich J. |
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fluorescence resonance energy transfer and complex formation between thiazole orange and various dye-dna conjugates: implications in signaling nucleic acid hybridization |
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Fluorescence Resonance Energy Transfer and Complex Formation Between Thiazole Orange and Various Dye-DNA Conjugates: Implications in Signaling Nucleic Acid Hybridization |
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Abstract Fluorescence resonance energy transfer (FRET) was investigated between the intercalating dye thiazole orange (TO), and the dyes Cyanine 3 (Cy3), Cyanine 5 (Cy5), Carboxytetramethyl Rhodamine (TAMRA), Iowa Black FQ (IabFQ), and Iowa Black RQ (IabRQ), which were covalently immobilized at the end of dsDNA oligonucleotides. In addition to determining that TO was an effective energy donor, FRET efficiency data obtained from fluorescence lifetime measurements indicated that TO intercalated near the middle of the 19mer oligonucleotide sequence that was used in this study. Discrepancies in FRET efficiencies obtained from intensity and lifetime measurements led to the investigation of non-fluorescent complex formation between TAMRA and modified TO. The hydrophobicity of TO was modified by the addition of either an alkyl or polyethylene glycol (PEG) side-chain to study effects of dimer and aggregate formation. It was found that at stoichiometric excesses of modified TO, fluorescence quenching of TAMRA was observed, and that this could be correlated to the hydrophobicity of a TO-chain species. The TAMRA:TO-chain association constant for the TO-alkyl system was 0.043±0.002 $ M^{−1} $, while that obtained for the TO-PEG was 0.037±0.002 $ M^{−1} $. From the perspective of method development for the transduction of hybridization events, we present and evaluate a variety of schemes based on energy transfer between TO and an acceptor dye, and discuss the implications of complex formation in such schemes. © Springer Science+Business Media, Inc. 2006 |
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Abstract Fluorescence resonance energy transfer (FRET) was investigated between the intercalating dye thiazole orange (TO), and the dyes Cyanine 3 (Cy3), Cyanine 5 (Cy5), Carboxytetramethyl Rhodamine (TAMRA), Iowa Black FQ (IabFQ), and Iowa Black RQ (IabRQ), which were covalently immobilized at the end of dsDNA oligonucleotides. In addition to determining that TO was an effective energy donor, FRET efficiency data obtained from fluorescence lifetime measurements indicated that TO intercalated near the middle of the 19mer oligonucleotide sequence that was used in this study. Discrepancies in FRET efficiencies obtained from intensity and lifetime measurements led to the investigation of non-fluorescent complex formation between TAMRA and modified TO. The hydrophobicity of TO was modified by the addition of either an alkyl or polyethylene glycol (PEG) side-chain to study effects of dimer and aggregate formation. It was found that at stoichiometric excesses of modified TO, fluorescence quenching of TAMRA was observed, and that this could be correlated to the hydrophobicity of a TO-chain species. The TAMRA:TO-chain association constant for the TO-alkyl system was 0.043±0.002 $ M^{−1} $, while that obtained for the TO-PEG was 0.037±0.002 $ M^{−1} $. From the perspective of method development for the transduction of hybridization events, we present and evaluate a variety of schemes based on energy transfer between TO and an acceptor dye, and discuss the implications of complex formation in such schemes. © Springer Science+Business Media, Inc. 2006 |
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Abstract Fluorescence resonance energy transfer (FRET) was investigated between the intercalating dye thiazole orange (TO), and the dyes Cyanine 3 (Cy3), Cyanine 5 (Cy5), Carboxytetramethyl Rhodamine (TAMRA), Iowa Black FQ (IabFQ), and Iowa Black RQ (IabRQ), which were covalently immobilized at the end of dsDNA oligonucleotides. In addition to determining that TO was an effective energy donor, FRET efficiency data obtained from fluorescence lifetime measurements indicated that TO intercalated near the middle of the 19mer oligonucleotide sequence that was used in this study. Discrepancies in FRET efficiencies obtained from intensity and lifetime measurements led to the investigation of non-fluorescent complex formation between TAMRA and modified TO. The hydrophobicity of TO was modified by the addition of either an alkyl or polyethylene glycol (PEG) side-chain to study effects of dimer and aggregate formation. It was found that at stoichiometric excesses of modified TO, fluorescence quenching of TAMRA was observed, and that this could be correlated to the hydrophobicity of a TO-chain species. The TAMRA:TO-chain association constant for the TO-alkyl system was 0.043±0.002 $ M^{−1} $, while that obtained for the TO-PEG was 0.037±0.002 $ M^{−1} $. From the perspective of method development for the transduction of hybridization events, we present and evaluate a variety of schemes based on energy transfer between TO and an acceptor dye, and discuss the implications of complex formation in such schemes. © Springer Science+Business Media, Inc. 2006 |
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Fluorescence Resonance Energy Transfer and Complex Formation Between Thiazole Orange and Various Dye-DNA Conjugates: Implications in Signaling Nucleic Acid Hybridization |
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