Simultaneous time- and spectrum-resolved multifocal multiphoton microscopy

Abstract We present a novel multifocal multiphoton microscope that is based on a sampling imaging technique using a microlens array, a prism for two-dimensionalspectral dispersion, and a specially designed streak camera to provide simultaneous time- and spectrum-resolved fluorescence microscopy. We...
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Autor*in:	Liu, L. [verfasserIn] Qu, J. Lin, Z. Wang, L. Fu, Z. Guo, B. Niu, H.

Format:	Artikel
Sprache:	Englisch

Erschienen:	2006

Schlagwörter:	Sample Stage Streak Camera Microlens Array Tissue Slide Simultaneous Time

Systematik:	UA 9001

Anmerkung:	© Springer-Verlag 2006

Übergeordnetes Werk:	Enthalten in: Applied physics. B, Lasers and optics - Springer-Verlag, 1981, 84(2006), 3 vom: 28. Juni, Seite 379-383
Übergeordnetes Werk:	volume:84 ; year:2006 ; number:3 ; day:28 ; month:06 ; pages:379-383

Links:	Volltext

DOI / URN:	10.1007/s00340-006-2314-y

Katalog-ID:	OLC2074284985

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allfields	10.1007/s00340-006-2314-y doi (DE-627)OLC2074284985 (DE-He213)s00340-006-2314-y-p DE-627 ger DE-627 rakwb eng 530 620 VZ 530 VZ UA 9001 VZ rvk Liu, L. verfasserin aut Simultaneous time- and spectrum-resolved multifocal multiphoton microscopy 2006 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag 2006 Abstract We present a novel multifocal multiphoton microscope that is based on a sampling imaging technique using a microlens array, a prism for two-dimensionalspectral dispersion, and a specially designed streak camera to provide simultaneous time- and spectrum-resolved fluorescence microscopy. We split the near-infrared light of a mode-locked titanium:sapphire femtosecond laser into an array of beams that are transformed into an array of high-aperture foci at the sample. A time- and spectrum-resolved image of 3×3 foci on the sample can be obtained with a snapshot. By translating the sample stage laterally and axially and implementing a dedicated image reconstruction algorithm in the control system of the instrument, we demonstrate the acquisition of a five-dimensional data set combining lifetime and spectral resolutions in biological fluorescence imaging. Sample Stage Streak Camera Microlens Array Tissue Slide Simultaneous Time Qu, J. aut Lin, Z. aut Wang, L. aut Fu, Z. aut Guo, B. aut Niu, H. aut Enthalten in Applied physics. B, Lasers and optics Springer-Verlag, 1981 84(2006), 3 vom: 28. Juni, Seite 379-383 (DE-627)130297682 (DE-600)579693-3 (DE-576)015877272 0946-2171 nnns volume:84 year:2006 number:3 day:28 month:06 pages:379-383 https://doi.org/10.1007/s00340-006-2314-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-PHY GBV_ILN_21 GBV_ILN_22 GBV_ILN_24 GBV_ILN_30 GBV_ILN_31 GBV_ILN_40 GBV_ILN_60 GBV_ILN_70 GBV_ILN_130 GBV_ILN_170 GBV_ILN_285 GBV_ILN_602 GBV_ILN_2010 GBV_ILN_2018 GBV_ILN_2021 GBV_ILN_4036 GBV_ILN_4116 GBV_ILN_4126 GBV_ILN_4266 GBV_ILN_4277 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4323 UA 9001 AR 84 2006 3 28 06 379-383
spelling	10.1007/s00340-006-2314-y doi (DE-627)OLC2074284985 (DE-He213)s00340-006-2314-y-p DE-627 ger DE-627 rakwb eng 530 620 VZ 530 VZ UA 9001 VZ rvk Liu, L. verfasserin aut Simultaneous time- and spectrum-resolved multifocal multiphoton microscopy 2006 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag 2006 Abstract We present a novel multifocal multiphoton microscope that is based on a sampling imaging technique using a microlens array, a prism for two-dimensionalspectral dispersion, and a specially designed streak camera to provide simultaneous time- and spectrum-resolved fluorescence microscopy. We split the near-infrared light of a mode-locked titanium:sapphire femtosecond laser into an array of beams that are transformed into an array of high-aperture foci at the sample. A time- and spectrum-resolved image of 3×3 foci on the sample can be obtained with a snapshot. By translating the sample stage laterally and axially and implementing a dedicated image reconstruction algorithm in the control system of the instrument, we demonstrate the acquisition of a five-dimensional data set combining lifetime and spectral resolutions in biological fluorescence imaging. Sample Stage Streak Camera Microlens Array Tissue Slide Simultaneous Time Qu, J. aut Lin, Z. aut Wang, L. aut Fu, Z. aut Guo, B. aut Niu, H. aut Enthalten in Applied physics. B, Lasers and optics Springer-Verlag, 1981 84(2006), 3 vom: 28. Juni, Seite 379-383 (DE-627)130297682 (DE-600)579693-3 (DE-576)015877272 0946-2171 nnns volume:84 year:2006 number:3 day:28 month:06 pages:379-383 https://doi.org/10.1007/s00340-006-2314-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-PHY GBV_ILN_21 GBV_ILN_22 GBV_ILN_24 GBV_ILN_30 GBV_ILN_31 GBV_ILN_40 GBV_ILN_60 GBV_ILN_70 GBV_ILN_130 GBV_ILN_170 GBV_ILN_285 GBV_ILN_602 GBV_ILN_2010 GBV_ILN_2018 GBV_ILN_2021 GBV_ILN_4036 GBV_ILN_4116 GBV_ILN_4126 GBV_ILN_4266 GBV_ILN_4277 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4323 UA 9001 AR 84 2006 3 28 06 379-383
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allfieldsSound	10.1007/s00340-006-2314-y doi (DE-627)OLC2074284985 (DE-He213)s00340-006-2314-y-p DE-627 ger DE-627 rakwb eng 530 620 VZ 530 VZ UA 9001 VZ rvk Liu, L. verfasserin aut Simultaneous time- and spectrum-resolved multifocal multiphoton microscopy 2006 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag 2006 Abstract We present a novel multifocal multiphoton microscope that is based on a sampling imaging technique using a microlens array, a prism for two-dimensionalspectral dispersion, and a specially designed streak camera to provide simultaneous time- and spectrum-resolved fluorescence microscopy. We split the near-infrared light of a mode-locked titanium:sapphire femtosecond laser into an array of beams that are transformed into an array of high-aperture foci at the sample. A time- and spectrum-resolved image of 3×3 foci on the sample can be obtained with a snapshot. By translating the sample stage laterally and axially and implementing a dedicated image reconstruction algorithm in the control system of the instrument, we demonstrate the acquisition of a five-dimensional data set combining lifetime and spectral resolutions in biological fluorescence imaging. Sample Stage Streak Camera Microlens Array Tissue Slide Simultaneous Time Qu, J. aut Lin, Z. aut Wang, L. aut Fu, Z. aut Guo, B. aut Niu, H. aut Enthalten in Applied physics. B, Lasers and optics Springer-Verlag, 1981 84(2006), 3 vom: 28. Juni, Seite 379-383 (DE-627)130297682 (DE-600)579693-3 (DE-576)015877272 0946-2171 nnns volume:84 year:2006 number:3 day:28 month:06 pages:379-383 https://doi.org/10.1007/s00340-006-2314-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-PHY GBV_ILN_21 GBV_ILN_22 GBV_ILN_24 GBV_ILN_30 GBV_ILN_31 GBV_ILN_40 GBV_ILN_60 GBV_ILN_70 GBV_ILN_130 GBV_ILN_170 GBV_ILN_285 GBV_ILN_602 GBV_ILN_2010 GBV_ILN_2018 GBV_ILN_2021 GBV_ILN_4036 GBV_ILN_4116 GBV_ILN_4126 GBV_ILN_4266 GBV_ILN_4277 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4323 UA 9001 AR 84 2006 3 28 06 379-383
language	English
source	Enthalten in Applied physics. B, Lasers and optics 84(2006), 3 vom: 28. Juni, Seite 379-383 volume:84 year:2006 number:3 day:28 month:06 pages:379-383
sourceStr	Enthalten in Applied physics. B, Lasers and optics 84(2006), 3 vom: 28. Juni, Seite 379-383 volume:84 year:2006 number:3 day:28 month:06 pages:379-383
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topic_facet	Sample Stage Streak Camera Microlens Array Tissue Slide Simultaneous Time
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authorswithroles_txt_mv	Liu, L. @@aut@@ Qu, J. @@aut@@ Lin, Z. @@aut@@ Wang, L. @@aut@@ Fu, Z. @@aut@@ Guo, B. @@aut@@ Niu, H. @@aut@@
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author	Liu, L.
spellingShingle	Liu, L. ddc 530 rvk UA 9001 misc Sample Stage misc Streak Camera misc Microlens Array misc Tissue Slide misc Simultaneous Time Simultaneous time- and spectrum-resolved multifocal multiphoton microscopy
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topic_title	530 620 VZ 530 VZ UA 9001 VZ rvk Simultaneous time- and spectrum-resolved multifocal multiphoton microscopy Sample Stage Streak Camera Microlens Array Tissue Slide Simultaneous Time
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title	Simultaneous time- and spectrum-resolved multifocal multiphoton microscopy
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title_full	Simultaneous time- and spectrum-resolved multifocal multiphoton microscopy
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title_sort	simultaneous time- and spectrum-resolved multifocal multiphoton microscopy
title_auth	Simultaneous time- and spectrum-resolved multifocal multiphoton microscopy
abstract	Abstract We present a novel multifocal multiphoton microscope that is based on a sampling imaging technique using a microlens array, a prism for two-dimensionalspectral dispersion, and a specially designed streak camera to provide simultaneous time- and spectrum-resolved fluorescence microscopy. We split the near-infrared light of a mode-locked titanium:sapphire femtosecond laser into an array of beams that are transformed into an array of high-aperture foci at the sample. A time- and spectrum-resolved image of 3×3 foci on the sample can be obtained with a snapshot. By translating the sample stage laterally and axially and implementing a dedicated image reconstruction algorithm in the control system of the instrument, we demonstrate the acquisition of a five-dimensional data set combining lifetime and spectral resolutions in biological fluorescence imaging. © Springer-Verlag 2006
abstractGer	Abstract We present a novel multifocal multiphoton microscope that is based on a sampling imaging technique using a microlens array, a prism for two-dimensionalspectral dispersion, and a specially designed streak camera to provide simultaneous time- and spectrum-resolved fluorescence microscopy. We split the near-infrared light of a mode-locked titanium:sapphire femtosecond laser into an array of beams that are transformed into an array of high-aperture foci at the sample. A time- and spectrum-resolved image of 3×3 foci on the sample can be obtained with a snapshot. By translating the sample stage laterally and axially and implementing a dedicated image reconstruction algorithm in the control system of the instrument, we demonstrate the acquisition of a five-dimensional data set combining lifetime and spectral resolutions in biological fluorescence imaging. © Springer-Verlag 2006
abstract_unstemmed	Abstract We present a novel multifocal multiphoton microscope that is based on a sampling imaging technique using a microlens array, a prism for two-dimensionalspectral dispersion, and a specially designed streak camera to provide simultaneous time- and spectrum-resolved fluorescence microscopy. We split the near-infrared light of a mode-locked titanium:sapphire femtosecond laser into an array of beams that are transformed into an array of high-aperture foci at the sample. A time- and spectrum-resolved image of 3×3 foci on the sample can be obtained with a snapshot. By translating the sample stage laterally and axially and implementing a dedicated image reconstruction algorithm in the control system of the instrument, we demonstrate the acquisition of a five-dimensional data set combining lifetime and spectral resolutions in biological fluorescence imaging. © Springer-Verlag 2006
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title_short	Simultaneous time- and spectrum-resolved multifocal multiphoton microscopy
url	https://doi.org/10.1007/s00340-006-2314-y
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author2	Qu, J. Lin, Z. Wang, L. Fu, Z. Guo, B. Niu, H.
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