Clonogenic survival of human keratinocytes and rodent fibroblasts after irradiation with 25 kV x-rays
Abstract Low energy x-rays ($ E_{ph} $ ≤50 keV) are widely used in diagnostic radiology and radiotherapy. However, data on their relative biological effectiveness (RBE) are scarce. Of particular importance for risk estimation are the RBE values of x-rays in the range which is commonly used in mammog...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Panteleeva, Anna [verfasserIn] |
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| Format: |
Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2003 |
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| Schlagwörter: |
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| Anmerkung: |
© Springer-Verlag 2003 |
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| Übergeordnetes Werk: |
Enthalten in: Radiation and environmental biophysics - Springer-Verlag, 1974, 42(2003), 2 vom: 26. Juni, Seite 95-100 |
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| Übergeordnetes Werk: |
volume:42 ; year:2003 ; number:2 ; day:26 ; month:06 ; pages:95-100 |
| Links: |
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| DOI / URN: |
10.1007/s00411-003-0199-2 |
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| Katalog-ID: |
OLC2075415455 |
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| 245 | 1 | 0 | |a Clonogenic survival of human keratinocytes and rodent fibroblasts after irradiation with 25 kV x-rays |
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| 520 | |a Abstract Low energy x-rays ($ E_{ph} $ ≤50 keV) are widely used in diagnostic radiology and radiotherapy. However, data on their relative biological effectiveness (RBE) are scarce. Of particular importance for risk estimation are the RBE values of x-rays in the range which is commonly used in mammography (10–30 keV). We have determined clonogenic survival after low-energy x-ray irradiation for three cell lines: primary human epidermal keratinocytes (HEKn), mouse fibroblasts (NIH/3T3) and Chinese hamster fibroblasts (V79). Experiments were performed with a 25 kV x-ray tube and compared to 200 kV x-rays as a reference. Compared to the effect of 200 kV x-rays, irradiation with 25 kV x-rays resulted in a decreased survival rate in the murine fibroblasts, but not in the human epithelial cell line. The RBE value was calculated for 10% surviving fraction. For HEKn cells, RBE was 1.33±0.27, for NIH/3T3 cells 1.25±0.07 and for V79 cells 1.10±0.09, respectively. No consistently increased RBE was observed in the various cell lines. Nevertheless, a potential of increased cytogenetic changes has to be considered for risk estimation of low-energy x-rays. | ||
| 650 | 4 | |a Relative Biological Effectiveness | |
| 650 | 4 | |a Clonogenic Survival | |
| 650 | 4 | |a Chinese Hamster Fibroblast | |
| 650 | 4 | |a Ionisation Chamber Type | |
| 650 | 4 | |a Human Keratinocyte Growth Supplement | |
| 700 | 1 | |a Słonina, Dorota |4 aut | |
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| 700 | 1 | |a Spekl, Kathrin |4 aut | |
| 700 | 1 | |a Pawelke, Jörg |4 aut | |
| 700 | 1 | |a Hoinkis, Cordelia |4 aut | |
| 700 | 1 | |a Dörr, Wolfgang |4 aut | |
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10.1007/s00411-003-0199-2 doi (DE-627)OLC2075415455 (DE-He213)s00411-003-0199-2-p DE-627 ger DE-627 rakwb eng 570 530 VZ 12 ssgn BIODIV DE-30 fid Panteleeva, Anna verfasserin aut Clonogenic survival of human keratinocytes and rodent fibroblasts after irradiation with 25 kV x-rays 2003 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag 2003 Abstract Low energy x-rays ($ E_{ph} $ ≤50 keV) are widely used in diagnostic radiology and radiotherapy. However, data on their relative biological effectiveness (RBE) are scarce. Of particular importance for risk estimation are the RBE values of x-rays in the range which is commonly used in mammography (10–30 keV). We have determined clonogenic survival after low-energy x-ray irradiation for three cell lines: primary human epidermal keratinocytes (HEKn), mouse fibroblasts (NIH/3T3) and Chinese hamster fibroblasts (V79). Experiments were performed with a 25 kV x-ray tube and compared to 200 kV x-rays as a reference. Compared to the effect of 200 kV x-rays, irradiation with 25 kV x-rays resulted in a decreased survival rate in the murine fibroblasts, but not in the human epithelial cell line. The RBE value was calculated for 10% surviving fraction. For HEKn cells, RBE was 1.33±0.27, for NIH/3T3 cells 1.25±0.07 and for V79 cells 1.10±0.09, respectively. No consistently increased RBE was observed in the various cell lines. Nevertheless, a potential of increased cytogenetic changes has to be considered for risk estimation of low-energy x-rays. Relative Biological Effectiveness Clonogenic Survival Chinese Hamster Fibroblast Ionisation Chamber Type Human Keratinocyte Growth Supplement Słonina, Dorota aut Brankovic, Katarina aut Spekl, Kathrin aut Pawelke, Jörg aut Hoinkis, Cordelia aut Dörr, Wolfgang aut Enthalten in Radiation and environmental biophysics Springer-Verlag, 1974 42(2003), 2 vom: 26. Juni, Seite 95-100 (DE-627)129308528 (DE-600)124987-3 (DE-576)014507870 0301-634X nnns volume:42 year:2003 number:2 day:26 month:06 pages:95-100 https://doi.org/10.1007/s00411-003-0199-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-PHY SSG-OPC-GGO GBV_ILN_11 GBV_ILN_21 GBV_ILN_22 GBV_ILN_40 GBV_ILN_70 GBV_ILN_2018 GBV_ILN_4012 GBV_ILN_4082 GBV_ILN_4219 GBV_ILN_4277 AR 42 2003 2 26 06 95-100 |
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10.1007/s00411-003-0199-2 doi (DE-627)OLC2075415455 (DE-He213)s00411-003-0199-2-p DE-627 ger DE-627 rakwb eng 570 530 VZ 12 ssgn BIODIV DE-30 fid Panteleeva, Anna verfasserin aut Clonogenic survival of human keratinocytes and rodent fibroblasts after irradiation with 25 kV x-rays 2003 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag 2003 Abstract Low energy x-rays ($ E_{ph} $ ≤50 keV) are widely used in diagnostic radiology and radiotherapy. However, data on their relative biological effectiveness (RBE) are scarce. Of particular importance for risk estimation are the RBE values of x-rays in the range which is commonly used in mammography (10–30 keV). We have determined clonogenic survival after low-energy x-ray irradiation for three cell lines: primary human epidermal keratinocytes (HEKn), mouse fibroblasts (NIH/3T3) and Chinese hamster fibroblasts (V79). Experiments were performed with a 25 kV x-ray tube and compared to 200 kV x-rays as a reference. Compared to the effect of 200 kV x-rays, irradiation with 25 kV x-rays resulted in a decreased survival rate in the murine fibroblasts, but not in the human epithelial cell line. The RBE value was calculated for 10% surviving fraction. For HEKn cells, RBE was 1.33±0.27, for NIH/3T3 cells 1.25±0.07 and for V79 cells 1.10±0.09, respectively. No consistently increased RBE was observed in the various cell lines. Nevertheless, a potential of increased cytogenetic changes has to be considered for risk estimation of low-energy x-rays. Relative Biological Effectiveness Clonogenic Survival Chinese Hamster Fibroblast Ionisation Chamber Type Human Keratinocyte Growth Supplement Słonina, Dorota aut Brankovic, Katarina aut Spekl, Kathrin aut Pawelke, Jörg aut Hoinkis, Cordelia aut Dörr, Wolfgang aut Enthalten in Radiation and environmental biophysics Springer-Verlag, 1974 42(2003), 2 vom: 26. Juni, Seite 95-100 (DE-627)129308528 (DE-600)124987-3 (DE-576)014507870 0301-634X nnns volume:42 year:2003 number:2 day:26 month:06 pages:95-100 https://doi.org/10.1007/s00411-003-0199-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-PHY SSG-OPC-GGO GBV_ILN_11 GBV_ILN_21 GBV_ILN_22 GBV_ILN_40 GBV_ILN_70 GBV_ILN_2018 GBV_ILN_4012 GBV_ILN_4082 GBV_ILN_4219 GBV_ILN_4277 AR 42 2003 2 26 06 95-100 |
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10.1007/s00411-003-0199-2 doi (DE-627)OLC2075415455 (DE-He213)s00411-003-0199-2-p DE-627 ger DE-627 rakwb eng 570 530 VZ 12 ssgn BIODIV DE-30 fid Panteleeva, Anna verfasserin aut Clonogenic survival of human keratinocytes and rodent fibroblasts after irradiation with 25 kV x-rays 2003 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag 2003 Abstract Low energy x-rays ($ E_{ph} $ ≤50 keV) are widely used in diagnostic radiology and radiotherapy. However, data on their relative biological effectiveness (RBE) are scarce. Of particular importance for risk estimation are the RBE values of x-rays in the range which is commonly used in mammography (10–30 keV). We have determined clonogenic survival after low-energy x-ray irradiation for three cell lines: primary human epidermal keratinocytes (HEKn), mouse fibroblasts (NIH/3T3) and Chinese hamster fibroblasts (V79). Experiments were performed with a 25 kV x-ray tube and compared to 200 kV x-rays as a reference. Compared to the effect of 200 kV x-rays, irradiation with 25 kV x-rays resulted in a decreased survival rate in the murine fibroblasts, but not in the human epithelial cell line. The RBE value was calculated for 10% surviving fraction. For HEKn cells, RBE was 1.33±0.27, for NIH/3T3 cells 1.25±0.07 and for V79 cells 1.10±0.09, respectively. No consistently increased RBE was observed in the various cell lines. Nevertheless, a potential of increased cytogenetic changes has to be considered for risk estimation of low-energy x-rays. Relative Biological Effectiveness Clonogenic Survival Chinese Hamster Fibroblast Ionisation Chamber Type Human Keratinocyte Growth Supplement Słonina, Dorota aut Brankovic, Katarina aut Spekl, Kathrin aut Pawelke, Jörg aut Hoinkis, Cordelia aut Dörr, Wolfgang aut Enthalten in Radiation and environmental biophysics Springer-Verlag, 1974 42(2003), 2 vom: 26. Juni, Seite 95-100 (DE-627)129308528 (DE-600)124987-3 (DE-576)014507870 0301-634X nnns volume:42 year:2003 number:2 day:26 month:06 pages:95-100 https://doi.org/10.1007/s00411-003-0199-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-PHY SSG-OPC-GGO GBV_ILN_11 GBV_ILN_21 GBV_ILN_22 GBV_ILN_40 GBV_ILN_70 GBV_ILN_2018 GBV_ILN_4012 GBV_ILN_4082 GBV_ILN_4219 GBV_ILN_4277 AR 42 2003 2 26 06 95-100 |
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10.1007/s00411-003-0199-2 doi (DE-627)OLC2075415455 (DE-He213)s00411-003-0199-2-p DE-627 ger DE-627 rakwb eng 570 530 VZ 12 ssgn BIODIV DE-30 fid Panteleeva, Anna verfasserin aut Clonogenic survival of human keratinocytes and rodent fibroblasts after irradiation with 25 kV x-rays 2003 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag 2003 Abstract Low energy x-rays ($ E_{ph} $ ≤50 keV) are widely used in diagnostic radiology and radiotherapy. However, data on their relative biological effectiveness (RBE) are scarce. Of particular importance for risk estimation are the RBE values of x-rays in the range which is commonly used in mammography (10–30 keV). We have determined clonogenic survival after low-energy x-ray irradiation for three cell lines: primary human epidermal keratinocytes (HEKn), mouse fibroblasts (NIH/3T3) and Chinese hamster fibroblasts (V79). Experiments were performed with a 25 kV x-ray tube and compared to 200 kV x-rays as a reference. Compared to the effect of 200 kV x-rays, irradiation with 25 kV x-rays resulted in a decreased survival rate in the murine fibroblasts, but not in the human epithelial cell line. The RBE value was calculated for 10% surviving fraction. For HEKn cells, RBE was 1.33±0.27, for NIH/3T3 cells 1.25±0.07 and for V79 cells 1.10±0.09, respectively. No consistently increased RBE was observed in the various cell lines. Nevertheless, a potential of increased cytogenetic changes has to be considered for risk estimation of low-energy x-rays. Relative Biological Effectiveness Clonogenic Survival Chinese Hamster Fibroblast Ionisation Chamber Type Human Keratinocyte Growth Supplement Słonina, Dorota aut Brankovic, Katarina aut Spekl, Kathrin aut Pawelke, Jörg aut Hoinkis, Cordelia aut Dörr, Wolfgang aut Enthalten in Radiation and environmental biophysics Springer-Verlag, 1974 42(2003), 2 vom: 26. Juni, Seite 95-100 (DE-627)129308528 (DE-600)124987-3 (DE-576)014507870 0301-634X nnns volume:42 year:2003 number:2 day:26 month:06 pages:95-100 https://doi.org/10.1007/s00411-003-0199-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-PHY SSG-OPC-GGO GBV_ILN_11 GBV_ILN_21 GBV_ILN_22 GBV_ILN_40 GBV_ILN_70 GBV_ILN_2018 GBV_ILN_4012 GBV_ILN_4082 GBV_ILN_4219 GBV_ILN_4277 AR 42 2003 2 26 06 95-100 |
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Panteleeva, Anna @@aut@@ Słonina, Dorota @@aut@@ Brankovic, Katarina @@aut@@ Spekl, Kathrin @@aut@@ Pawelke, Jörg @@aut@@ Hoinkis, Cordelia @@aut@@ Dörr, Wolfgang @@aut@@ |
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clonogenic survival of human keratinocytes and rodent fibroblasts after irradiation with 25 kv x-rays |
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Clonogenic survival of human keratinocytes and rodent fibroblasts after irradiation with 25 kV x-rays |
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Abstract Low energy x-rays ($ E_{ph} $ ≤50 keV) are widely used in diagnostic radiology and radiotherapy. However, data on their relative biological effectiveness (RBE) are scarce. Of particular importance for risk estimation are the RBE values of x-rays in the range which is commonly used in mammography (10–30 keV). We have determined clonogenic survival after low-energy x-ray irradiation for three cell lines: primary human epidermal keratinocytes (HEKn), mouse fibroblasts (NIH/3T3) and Chinese hamster fibroblasts (V79). Experiments were performed with a 25 kV x-ray tube and compared to 200 kV x-rays as a reference. Compared to the effect of 200 kV x-rays, irradiation with 25 kV x-rays resulted in a decreased survival rate in the murine fibroblasts, but not in the human epithelial cell line. The RBE value was calculated for 10% surviving fraction. For HEKn cells, RBE was 1.33±0.27, for NIH/3T3 cells 1.25±0.07 and for V79 cells 1.10±0.09, respectively. No consistently increased RBE was observed in the various cell lines. Nevertheless, a potential of increased cytogenetic changes has to be considered for risk estimation of low-energy x-rays. © Springer-Verlag 2003 |
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Abstract Low energy x-rays ($ E_{ph} $ ≤50 keV) are widely used in diagnostic radiology and radiotherapy. However, data on their relative biological effectiveness (RBE) are scarce. Of particular importance for risk estimation are the RBE values of x-rays in the range which is commonly used in mammography (10–30 keV). We have determined clonogenic survival after low-energy x-ray irradiation for three cell lines: primary human epidermal keratinocytes (HEKn), mouse fibroblasts (NIH/3T3) and Chinese hamster fibroblasts (V79). Experiments were performed with a 25 kV x-ray tube and compared to 200 kV x-rays as a reference. Compared to the effect of 200 kV x-rays, irradiation with 25 kV x-rays resulted in a decreased survival rate in the murine fibroblasts, but not in the human epithelial cell line. The RBE value was calculated for 10% surviving fraction. For HEKn cells, RBE was 1.33±0.27, for NIH/3T3 cells 1.25±0.07 and for V79 cells 1.10±0.09, respectively. No consistently increased RBE was observed in the various cell lines. Nevertheless, a potential of increased cytogenetic changes has to be considered for risk estimation of low-energy x-rays. © Springer-Verlag 2003 |
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Abstract Low energy x-rays ($ E_{ph} $ ≤50 keV) are widely used in diagnostic radiology and radiotherapy. However, data on their relative biological effectiveness (RBE) are scarce. Of particular importance for risk estimation are the RBE values of x-rays in the range which is commonly used in mammography (10–30 keV). We have determined clonogenic survival after low-energy x-ray irradiation for three cell lines: primary human epidermal keratinocytes (HEKn), mouse fibroblasts (NIH/3T3) and Chinese hamster fibroblasts (V79). Experiments were performed with a 25 kV x-ray tube and compared to 200 kV x-rays as a reference. Compared to the effect of 200 kV x-rays, irradiation with 25 kV x-rays resulted in a decreased survival rate in the murine fibroblasts, but not in the human epithelial cell line. The RBE value was calculated for 10% surviving fraction. For HEKn cells, RBE was 1.33±0.27, for NIH/3T3 cells 1.25±0.07 and for V79 cells 1.10±0.09, respectively. No consistently increased RBE was observed in the various cell lines. Nevertheless, a potential of increased cytogenetic changes has to be considered for risk estimation of low-energy x-rays. © Springer-Verlag 2003 |
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