Detection of β-Lactam Antibiotics Based on Conjugated Antibody with Gold Nanorods by Localized Surface Plasmon Resonance Spectrometer
This study aimed to determine the β-lactam antibiotics (ampicillin, amoxicillin, penicillin G, oxacillin, and carbenicillin) using conjugated antibody along with gold nanorods (AuNRs). For this purpose, XRD, ATR-FTIR spectroscopy, transmission electron microscopy, and dynamic light scattering were u...
Ausführliche Beschreibung
Autor*in: |
Aghamirzaei, M. [verfasserIn] |
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Sprache: |
Englisch |
Erschienen: |
2022 |
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Schlagwörter: |
localized surface plasmon resonance spectrometer polyclonal antibody of β-lactam poly(4-styrenesulfonic acid) solution |
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Anmerkung: |
© Springer Science+Business Media, LLC, part of Springer Nature 2022 |
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Übergeordnetes Werk: |
Enthalten in: Journal of applied spectroscopy - Springer US, 1966, 89(2022), 2 vom: Mai, Seite 391-399 |
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Übergeordnetes Werk: |
volume:89 ; year:2022 ; number:2 ; month:05 ; pages:391-399 |
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DOI / URN: |
10.1007/s10812-022-01369-7 |
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Katalog-ID: |
OLC2078887463 |
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520 | |a This study aimed to determine the β-lactam antibiotics (ampicillin, amoxicillin, penicillin G, oxacillin, and carbenicillin) using conjugated antibody along with gold nanorods (AuNRs). For this purpose, XRD, ATR-FTIR spectroscopy, transmission electron microscopy, and dynamic light scattering were utilized to detect the crystallinity, to identify functional groups involved in the synthesis of AuNRs, and to measure the size of the AuNRs, respectively. In this regard, pH of 9 and a concentration of 9.6 μg of antibody at 1 mL poly(4-styrenesulfonic acid (PSS))-modified AuNRs solution were selected as the best levels of pH and concentration of antibody for the conjugation of antibody with PSS-modified AuNRs. Thereafter, the maximum wavelength rates of the PSS-modified AuNRs, conjugation of antibody with PSS-modified AuNRs, and detection of antibiotics (from 1 nM to 1 mM) with PSS-modified AuNRs–PAb were recorded using a microvolume spectrophotometer system. The results indicate that the LSPR absorption wavelength of PSS-modified AuNRs is red-shifted by increasing the concentration of β-lactam antibiotics. By increasing the concentrations of ampicillin, penicillin G, and carbenicillin, the maximum wavelength changed, and after the saturation of the antibiotic concentration, the curve reached a plateau. Correspondingly, this indicated that the antibody had a similar behavior in the detection of these antibiotics. However, regarding amoxicillin, the saturation concentration is much higher, which indicate that the antibody is more specific for its detection. In contrast, for oxacillin, saturation occurred immediately, demonstrating that the antibody had an extremely low detection capability for this antibiotic. Finally, the findings showed that the antibody was sensitive to 1 nM of five studied β-lactam antibiotics. | ||
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10.1007/s10812-022-01369-7 doi (DE-627)OLC2078887463 (DE-He213)s10812-022-01369-7-p DE-627 ger DE-627 rakwb eng 530 VZ 11 ssgn Aghamirzaei, M. verfasserin aut Detection of β-Lactam Antibiotics Based on Conjugated Antibody with Gold Nanorods by Localized Surface Plasmon Resonance Spectrometer 2022 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer Science+Business Media, LLC, part of Springer Nature 2022 This study aimed to determine the β-lactam antibiotics (ampicillin, amoxicillin, penicillin G, oxacillin, and carbenicillin) using conjugated antibody along with gold nanorods (AuNRs). For this purpose, XRD, ATR-FTIR spectroscopy, transmission electron microscopy, and dynamic light scattering were utilized to detect the crystallinity, to identify functional groups involved in the synthesis of AuNRs, and to measure the size of the AuNRs, respectively. In this regard, pH of 9 and a concentration of 9.6 μg of antibody at 1 mL poly(4-styrenesulfonic acid (PSS))-modified AuNRs solution were selected as the best levels of pH and concentration of antibody for the conjugation of antibody with PSS-modified AuNRs. Thereafter, the maximum wavelength rates of the PSS-modified AuNRs, conjugation of antibody with PSS-modified AuNRs, and detection of antibiotics (from 1 nM to 1 mM) with PSS-modified AuNRs–PAb were recorded using a microvolume spectrophotometer system. The results indicate that the LSPR absorption wavelength of PSS-modified AuNRs is red-shifted by increasing the concentration of β-lactam antibiotics. By increasing the concentrations of ampicillin, penicillin G, and carbenicillin, the maximum wavelength changed, and after the saturation of the antibiotic concentration, the curve reached a plateau. Correspondingly, this indicated that the antibody had a similar behavior in the detection of these antibiotics. However, regarding amoxicillin, the saturation concentration is much higher, which indicate that the antibody is more specific for its detection. In contrast, for oxacillin, saturation occurred immediately, demonstrating that the antibody had an extremely low detection capability for this antibiotic. Finally, the findings showed that the antibody was sensitive to 1 nM of five studied β-lactam antibiotics. localized surface plasmon resonance spectrometer β-lactam antibiotics polyclonal antibody of β-lactam gold nanorods poly(4-styrenesulfonic acid) solution cetyltrimethylammonium bromide conjugation Khiabani, M. S. aut Hamishehkar, H. aut Mokarram, R. R. aut Amjadi, M. aut Enthalten in Journal of applied spectroscopy Springer US, 1966 89(2022), 2 vom: Mai, Seite 391-399 (DE-627)129972495 (DE-600)410515-1 (DE-576)015535800 0021-9037 nnns volume:89 year:2022 number:2 month:05 pages:391-399 https://doi.org/10.1007/s10812-022-01369-7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-PHY SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 AR 89 2022 2 05 391-399 |
spelling |
10.1007/s10812-022-01369-7 doi (DE-627)OLC2078887463 (DE-He213)s10812-022-01369-7-p DE-627 ger DE-627 rakwb eng 530 VZ 11 ssgn Aghamirzaei, M. verfasserin aut Detection of β-Lactam Antibiotics Based on Conjugated Antibody with Gold Nanorods by Localized Surface Plasmon Resonance Spectrometer 2022 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer Science+Business Media, LLC, part of Springer Nature 2022 This study aimed to determine the β-lactam antibiotics (ampicillin, amoxicillin, penicillin G, oxacillin, and carbenicillin) using conjugated antibody along with gold nanorods (AuNRs). For this purpose, XRD, ATR-FTIR spectroscopy, transmission electron microscopy, and dynamic light scattering were utilized to detect the crystallinity, to identify functional groups involved in the synthesis of AuNRs, and to measure the size of the AuNRs, respectively. In this regard, pH of 9 and a concentration of 9.6 μg of antibody at 1 mL poly(4-styrenesulfonic acid (PSS))-modified AuNRs solution were selected as the best levels of pH and concentration of antibody for the conjugation of antibody with PSS-modified AuNRs. Thereafter, the maximum wavelength rates of the PSS-modified AuNRs, conjugation of antibody with PSS-modified AuNRs, and detection of antibiotics (from 1 nM to 1 mM) with PSS-modified AuNRs–PAb were recorded using a microvolume spectrophotometer system. The results indicate that the LSPR absorption wavelength of PSS-modified AuNRs is red-shifted by increasing the concentration of β-lactam antibiotics. By increasing the concentrations of ampicillin, penicillin G, and carbenicillin, the maximum wavelength changed, and after the saturation of the antibiotic concentration, the curve reached a plateau. Correspondingly, this indicated that the antibody had a similar behavior in the detection of these antibiotics. However, regarding amoxicillin, the saturation concentration is much higher, which indicate that the antibody is more specific for its detection. In contrast, for oxacillin, saturation occurred immediately, demonstrating that the antibody had an extremely low detection capability for this antibiotic. Finally, the findings showed that the antibody was sensitive to 1 nM of five studied β-lactam antibiotics. localized surface plasmon resonance spectrometer β-lactam antibiotics polyclonal antibody of β-lactam gold nanorods poly(4-styrenesulfonic acid) solution cetyltrimethylammonium bromide conjugation Khiabani, M. S. aut Hamishehkar, H. aut Mokarram, R. R. aut Amjadi, M. aut Enthalten in Journal of applied spectroscopy Springer US, 1966 89(2022), 2 vom: Mai, Seite 391-399 (DE-627)129972495 (DE-600)410515-1 (DE-576)015535800 0021-9037 nnns volume:89 year:2022 number:2 month:05 pages:391-399 https://doi.org/10.1007/s10812-022-01369-7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-PHY SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 AR 89 2022 2 05 391-399 |
allfields_unstemmed |
10.1007/s10812-022-01369-7 doi (DE-627)OLC2078887463 (DE-He213)s10812-022-01369-7-p DE-627 ger DE-627 rakwb eng 530 VZ 11 ssgn Aghamirzaei, M. verfasserin aut Detection of β-Lactam Antibiotics Based on Conjugated Antibody with Gold Nanorods by Localized Surface Plasmon Resonance Spectrometer 2022 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer Science+Business Media, LLC, part of Springer Nature 2022 This study aimed to determine the β-lactam antibiotics (ampicillin, amoxicillin, penicillin G, oxacillin, and carbenicillin) using conjugated antibody along with gold nanorods (AuNRs). For this purpose, XRD, ATR-FTIR spectroscopy, transmission electron microscopy, and dynamic light scattering were utilized to detect the crystallinity, to identify functional groups involved in the synthesis of AuNRs, and to measure the size of the AuNRs, respectively. In this regard, pH of 9 and a concentration of 9.6 μg of antibody at 1 mL poly(4-styrenesulfonic acid (PSS))-modified AuNRs solution were selected as the best levels of pH and concentration of antibody for the conjugation of antibody with PSS-modified AuNRs. Thereafter, the maximum wavelength rates of the PSS-modified AuNRs, conjugation of antibody with PSS-modified AuNRs, and detection of antibiotics (from 1 nM to 1 mM) with PSS-modified AuNRs–PAb were recorded using a microvolume spectrophotometer system. The results indicate that the LSPR absorption wavelength of PSS-modified AuNRs is red-shifted by increasing the concentration of β-lactam antibiotics. By increasing the concentrations of ampicillin, penicillin G, and carbenicillin, the maximum wavelength changed, and after the saturation of the antibiotic concentration, the curve reached a plateau. Correspondingly, this indicated that the antibody had a similar behavior in the detection of these antibiotics. However, regarding amoxicillin, the saturation concentration is much higher, which indicate that the antibody is more specific for its detection. In contrast, for oxacillin, saturation occurred immediately, demonstrating that the antibody had an extremely low detection capability for this antibiotic. Finally, the findings showed that the antibody was sensitive to 1 nM of five studied β-lactam antibiotics. localized surface plasmon resonance spectrometer β-lactam antibiotics polyclonal antibody of β-lactam gold nanorods poly(4-styrenesulfonic acid) solution cetyltrimethylammonium bromide conjugation Khiabani, M. S. aut Hamishehkar, H. aut Mokarram, R. R. aut Amjadi, M. aut Enthalten in Journal of applied spectroscopy Springer US, 1966 89(2022), 2 vom: Mai, Seite 391-399 (DE-627)129972495 (DE-600)410515-1 (DE-576)015535800 0021-9037 nnns volume:89 year:2022 number:2 month:05 pages:391-399 https://doi.org/10.1007/s10812-022-01369-7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-PHY SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 AR 89 2022 2 05 391-399 |
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10.1007/s10812-022-01369-7 doi (DE-627)OLC2078887463 (DE-He213)s10812-022-01369-7-p DE-627 ger DE-627 rakwb eng 530 VZ 11 ssgn Aghamirzaei, M. verfasserin aut Detection of β-Lactam Antibiotics Based on Conjugated Antibody with Gold Nanorods by Localized Surface Plasmon Resonance Spectrometer 2022 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer Science+Business Media, LLC, part of Springer Nature 2022 This study aimed to determine the β-lactam antibiotics (ampicillin, amoxicillin, penicillin G, oxacillin, and carbenicillin) using conjugated antibody along with gold nanorods (AuNRs). For this purpose, XRD, ATR-FTIR spectroscopy, transmission electron microscopy, and dynamic light scattering were utilized to detect the crystallinity, to identify functional groups involved in the synthesis of AuNRs, and to measure the size of the AuNRs, respectively. In this regard, pH of 9 and a concentration of 9.6 μg of antibody at 1 mL poly(4-styrenesulfonic acid (PSS))-modified AuNRs solution were selected as the best levels of pH and concentration of antibody for the conjugation of antibody with PSS-modified AuNRs. Thereafter, the maximum wavelength rates of the PSS-modified AuNRs, conjugation of antibody with PSS-modified AuNRs, and detection of antibiotics (from 1 nM to 1 mM) with PSS-modified AuNRs–PAb were recorded using a microvolume spectrophotometer system. The results indicate that the LSPR absorption wavelength of PSS-modified AuNRs is red-shifted by increasing the concentration of β-lactam antibiotics. By increasing the concentrations of ampicillin, penicillin G, and carbenicillin, the maximum wavelength changed, and after the saturation of the antibiotic concentration, the curve reached a plateau. Correspondingly, this indicated that the antibody had a similar behavior in the detection of these antibiotics. However, regarding amoxicillin, the saturation concentration is much higher, which indicate that the antibody is more specific for its detection. In contrast, for oxacillin, saturation occurred immediately, demonstrating that the antibody had an extremely low detection capability for this antibiotic. Finally, the findings showed that the antibody was sensitive to 1 nM of five studied β-lactam antibiotics. localized surface plasmon resonance spectrometer β-lactam antibiotics polyclonal antibody of β-lactam gold nanorods poly(4-styrenesulfonic acid) solution cetyltrimethylammonium bromide conjugation Khiabani, M. S. aut Hamishehkar, H. aut Mokarram, R. R. aut Amjadi, M. aut Enthalten in Journal of applied spectroscopy Springer US, 1966 89(2022), 2 vom: Mai, Seite 391-399 (DE-627)129972495 (DE-600)410515-1 (DE-576)015535800 0021-9037 nnns volume:89 year:2022 number:2 month:05 pages:391-399 https://doi.org/10.1007/s10812-022-01369-7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-PHY SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 AR 89 2022 2 05 391-399 |
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10.1007/s10812-022-01369-7 doi (DE-627)OLC2078887463 (DE-He213)s10812-022-01369-7-p DE-627 ger DE-627 rakwb eng 530 VZ 11 ssgn Aghamirzaei, M. verfasserin aut Detection of β-Lactam Antibiotics Based on Conjugated Antibody with Gold Nanorods by Localized Surface Plasmon Resonance Spectrometer 2022 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer Science+Business Media, LLC, part of Springer Nature 2022 This study aimed to determine the β-lactam antibiotics (ampicillin, amoxicillin, penicillin G, oxacillin, and carbenicillin) using conjugated antibody along with gold nanorods (AuNRs). For this purpose, XRD, ATR-FTIR spectroscopy, transmission electron microscopy, and dynamic light scattering were utilized to detect the crystallinity, to identify functional groups involved in the synthesis of AuNRs, and to measure the size of the AuNRs, respectively. In this regard, pH of 9 and a concentration of 9.6 μg of antibody at 1 mL poly(4-styrenesulfonic acid (PSS))-modified AuNRs solution were selected as the best levels of pH and concentration of antibody for the conjugation of antibody with PSS-modified AuNRs. Thereafter, the maximum wavelength rates of the PSS-modified AuNRs, conjugation of antibody with PSS-modified AuNRs, and detection of antibiotics (from 1 nM to 1 mM) with PSS-modified AuNRs–PAb were recorded using a microvolume spectrophotometer system. The results indicate that the LSPR absorption wavelength of PSS-modified AuNRs is red-shifted by increasing the concentration of β-lactam antibiotics. By increasing the concentrations of ampicillin, penicillin G, and carbenicillin, the maximum wavelength changed, and after the saturation of the antibiotic concentration, the curve reached a plateau. Correspondingly, this indicated that the antibody had a similar behavior in the detection of these antibiotics. However, regarding amoxicillin, the saturation concentration is much higher, which indicate that the antibody is more specific for its detection. In contrast, for oxacillin, saturation occurred immediately, demonstrating that the antibody had an extremely low detection capability for this antibiotic. Finally, the findings showed that the antibody was sensitive to 1 nM of five studied β-lactam antibiotics. localized surface plasmon resonance spectrometer β-lactam antibiotics polyclonal antibody of β-lactam gold nanorods poly(4-styrenesulfonic acid) solution cetyltrimethylammonium bromide conjugation Khiabani, M. S. aut Hamishehkar, H. aut Mokarram, R. R. aut Amjadi, M. aut Enthalten in Journal of applied spectroscopy Springer US, 1966 89(2022), 2 vom: Mai, Seite 391-399 (DE-627)129972495 (DE-600)410515-1 (DE-576)015535800 0021-9037 nnns volume:89 year:2022 number:2 month:05 pages:391-399 https://doi.org/10.1007/s10812-022-01369-7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-PHY SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 AR 89 2022 2 05 391-399 |
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detection of β-lactam antibiotics based on conjugated antibody with gold nanorods by localized surface plasmon resonance spectrometer |
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Detection of β-Lactam Antibiotics Based on Conjugated Antibody with Gold Nanorods by Localized Surface Plasmon Resonance Spectrometer |
abstract |
This study aimed to determine the β-lactam antibiotics (ampicillin, amoxicillin, penicillin G, oxacillin, and carbenicillin) using conjugated antibody along with gold nanorods (AuNRs). For this purpose, XRD, ATR-FTIR spectroscopy, transmission electron microscopy, and dynamic light scattering were utilized to detect the crystallinity, to identify functional groups involved in the synthesis of AuNRs, and to measure the size of the AuNRs, respectively. In this regard, pH of 9 and a concentration of 9.6 μg of antibody at 1 mL poly(4-styrenesulfonic acid (PSS))-modified AuNRs solution were selected as the best levels of pH and concentration of antibody for the conjugation of antibody with PSS-modified AuNRs. Thereafter, the maximum wavelength rates of the PSS-modified AuNRs, conjugation of antibody with PSS-modified AuNRs, and detection of antibiotics (from 1 nM to 1 mM) with PSS-modified AuNRs–PAb were recorded using a microvolume spectrophotometer system. The results indicate that the LSPR absorption wavelength of PSS-modified AuNRs is red-shifted by increasing the concentration of β-lactam antibiotics. By increasing the concentrations of ampicillin, penicillin G, and carbenicillin, the maximum wavelength changed, and after the saturation of the antibiotic concentration, the curve reached a plateau. Correspondingly, this indicated that the antibody had a similar behavior in the detection of these antibiotics. However, regarding amoxicillin, the saturation concentration is much higher, which indicate that the antibody is more specific for its detection. In contrast, for oxacillin, saturation occurred immediately, demonstrating that the antibody had an extremely low detection capability for this antibiotic. Finally, the findings showed that the antibody was sensitive to 1 nM of five studied β-lactam antibiotics. © Springer Science+Business Media, LLC, part of Springer Nature 2022 |
abstractGer |
This study aimed to determine the β-lactam antibiotics (ampicillin, amoxicillin, penicillin G, oxacillin, and carbenicillin) using conjugated antibody along with gold nanorods (AuNRs). For this purpose, XRD, ATR-FTIR spectroscopy, transmission electron microscopy, and dynamic light scattering were utilized to detect the crystallinity, to identify functional groups involved in the synthesis of AuNRs, and to measure the size of the AuNRs, respectively. In this regard, pH of 9 and a concentration of 9.6 μg of antibody at 1 mL poly(4-styrenesulfonic acid (PSS))-modified AuNRs solution were selected as the best levels of pH and concentration of antibody for the conjugation of antibody with PSS-modified AuNRs. Thereafter, the maximum wavelength rates of the PSS-modified AuNRs, conjugation of antibody with PSS-modified AuNRs, and detection of antibiotics (from 1 nM to 1 mM) with PSS-modified AuNRs–PAb were recorded using a microvolume spectrophotometer system. The results indicate that the LSPR absorption wavelength of PSS-modified AuNRs is red-shifted by increasing the concentration of β-lactam antibiotics. By increasing the concentrations of ampicillin, penicillin G, and carbenicillin, the maximum wavelength changed, and after the saturation of the antibiotic concentration, the curve reached a plateau. Correspondingly, this indicated that the antibody had a similar behavior in the detection of these antibiotics. However, regarding amoxicillin, the saturation concentration is much higher, which indicate that the antibody is more specific for its detection. In contrast, for oxacillin, saturation occurred immediately, demonstrating that the antibody had an extremely low detection capability for this antibiotic. Finally, the findings showed that the antibody was sensitive to 1 nM of five studied β-lactam antibiotics. © Springer Science+Business Media, LLC, part of Springer Nature 2022 |
abstract_unstemmed |
This study aimed to determine the β-lactam antibiotics (ampicillin, amoxicillin, penicillin G, oxacillin, and carbenicillin) using conjugated antibody along with gold nanorods (AuNRs). For this purpose, XRD, ATR-FTIR spectroscopy, transmission electron microscopy, and dynamic light scattering were utilized to detect the crystallinity, to identify functional groups involved in the synthesis of AuNRs, and to measure the size of the AuNRs, respectively. In this regard, pH of 9 and a concentration of 9.6 μg of antibody at 1 mL poly(4-styrenesulfonic acid (PSS))-modified AuNRs solution were selected as the best levels of pH and concentration of antibody for the conjugation of antibody with PSS-modified AuNRs. Thereafter, the maximum wavelength rates of the PSS-modified AuNRs, conjugation of antibody with PSS-modified AuNRs, and detection of antibiotics (from 1 nM to 1 mM) with PSS-modified AuNRs–PAb were recorded using a microvolume spectrophotometer system. The results indicate that the LSPR absorption wavelength of PSS-modified AuNRs is red-shifted by increasing the concentration of β-lactam antibiotics. By increasing the concentrations of ampicillin, penicillin G, and carbenicillin, the maximum wavelength changed, and after the saturation of the antibiotic concentration, the curve reached a plateau. Correspondingly, this indicated that the antibody had a similar behavior in the detection of these antibiotics. However, regarding amoxicillin, the saturation concentration is much higher, which indicate that the antibody is more specific for its detection. In contrast, for oxacillin, saturation occurred immediately, demonstrating that the antibody had an extremely low detection capability for this antibiotic. Finally, the findings showed that the antibody was sensitive to 1 nM of five studied β-lactam antibiotics. © Springer Science+Business Media, LLC, part of Springer Nature 2022 |
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Detection of β-Lactam Antibiotics Based on Conjugated Antibody with Gold Nanorods by Localized Surface Plasmon Resonance Spectrometer |
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