Saturated confocal fluorescence microscopy with linear polarization modulation
Abstract In confocal scanning fluorescence microscopy, the effective modulation transfer function with Gaussian plane wave illumination covers very few high-frequency components, which prohibits further improvement of the spatial resolution. In this study, we propose saturated confocal scanning fluo...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Le, Vannhu [verfasserIn] |
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| Format: |
Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2022 |
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| Anmerkung: |
© The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. |
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| Übergeordnetes Werk: |
Enthalten in: Optical and quantum electronics - Springer US, 1975, 55(2022), 1 vom: 16. Dez. |
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| Übergeordnetes Werk: |
volume:55 ; year:2022 ; number:1 ; day:16 ; month:12 |
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| DOI / URN: |
10.1007/s11082-022-04386-0 |
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| 520 | |a Abstract In confocal scanning fluorescence microscopy, the effective modulation transfer function with Gaussian plane wave illumination covers very few high-frequency components, which prohibits further improvement of the spatial resolution. In this study, we propose saturated confocal scanning fluorescence microscopy with linear polarization to achieve super-resolution imaging. In saturated confocal scanning fluorescence microscopy with linear polarization, the effective modulation transfer function in the Fourier domain is extended in comparison with that of Gaussian plane wave illumination. The digital algorithm is adapted to retrieve the super-resolved image from the modulated recordings. The simulation results demonstrated that saturated confocal scanning fluorescence microscopy with linear polarization could be used to increase the resolution in confocal scanning fluorescence microscopy. | ||
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10.1007/s11082-022-04386-0 doi (DE-627)OLC2080168460 (DE-He213)s11082-022-04386-0-p DE-627 ger DE-627 rakwb eng 500 620 VZ Le, Vannhu verfasserin aut Saturated confocal fluorescence microscopy with linear polarization modulation 2022 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. Abstract In confocal scanning fluorescence microscopy, the effective modulation transfer function with Gaussian plane wave illumination covers very few high-frequency components, which prohibits further improvement of the spatial resolution. In this study, we propose saturated confocal scanning fluorescence microscopy with linear polarization to achieve super-resolution imaging. In saturated confocal scanning fluorescence microscopy with linear polarization, the effective modulation transfer function in the Fourier domain is extended in comparison with that of Gaussian plane wave illumination. The digital algorithm is adapted to retrieve the super-resolved image from the modulated recordings. The simulation results demonstrated that saturated confocal scanning fluorescence microscopy with linear polarization could be used to increase the resolution in confocal scanning fluorescence microscopy. Confocal fluorescence microscopy (CFM) Super-resolution RL Algorithm Enthalten in Optical and quantum electronics Springer US, 1975 55(2022), 1 vom: 16. Dez. (DE-627)129419540 (DE-600)189950-8 (DE-576)014796139 0306-8919 nnns volume:55 year:2022 number:1 day:16 month:12 https://doi.org/10.1007/s11082-022-04386-0 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-PHY AR 55 2022 1 16 12 |
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10.1007/s11082-022-04386-0 doi (DE-627)OLC2080168460 (DE-He213)s11082-022-04386-0-p DE-627 ger DE-627 rakwb eng 500 620 VZ Le, Vannhu verfasserin aut Saturated confocal fluorescence microscopy with linear polarization modulation 2022 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. Abstract In confocal scanning fluorescence microscopy, the effective modulation transfer function with Gaussian plane wave illumination covers very few high-frequency components, which prohibits further improvement of the spatial resolution. In this study, we propose saturated confocal scanning fluorescence microscopy with linear polarization to achieve super-resolution imaging. In saturated confocal scanning fluorescence microscopy with linear polarization, the effective modulation transfer function in the Fourier domain is extended in comparison with that of Gaussian plane wave illumination. The digital algorithm is adapted to retrieve the super-resolved image from the modulated recordings. The simulation results demonstrated that saturated confocal scanning fluorescence microscopy with linear polarization could be used to increase the resolution in confocal scanning fluorescence microscopy. Confocal fluorescence microscopy (CFM) Super-resolution RL Algorithm Enthalten in Optical and quantum electronics Springer US, 1975 55(2022), 1 vom: 16. Dez. (DE-627)129419540 (DE-600)189950-8 (DE-576)014796139 0306-8919 nnns volume:55 year:2022 number:1 day:16 month:12 https://doi.org/10.1007/s11082-022-04386-0 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-PHY AR 55 2022 1 16 12 |
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10.1007/s11082-022-04386-0 doi (DE-627)OLC2080168460 (DE-He213)s11082-022-04386-0-p DE-627 ger DE-627 rakwb eng 500 620 VZ Le, Vannhu verfasserin aut Saturated confocal fluorescence microscopy with linear polarization modulation 2022 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. Abstract In confocal scanning fluorescence microscopy, the effective modulation transfer function with Gaussian plane wave illumination covers very few high-frequency components, which prohibits further improvement of the spatial resolution. In this study, we propose saturated confocal scanning fluorescence microscopy with linear polarization to achieve super-resolution imaging. In saturated confocal scanning fluorescence microscopy with linear polarization, the effective modulation transfer function in the Fourier domain is extended in comparison with that of Gaussian plane wave illumination. The digital algorithm is adapted to retrieve the super-resolved image from the modulated recordings. The simulation results demonstrated that saturated confocal scanning fluorescence microscopy with linear polarization could be used to increase the resolution in confocal scanning fluorescence microscopy. Confocal fluorescence microscopy (CFM) Super-resolution RL Algorithm Enthalten in Optical and quantum electronics Springer US, 1975 55(2022), 1 vom: 16. Dez. (DE-627)129419540 (DE-600)189950-8 (DE-576)014796139 0306-8919 nnns volume:55 year:2022 number:1 day:16 month:12 https://doi.org/10.1007/s11082-022-04386-0 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-PHY AR 55 2022 1 16 12 |
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10.1007/s11082-022-04386-0 doi (DE-627)OLC2080168460 (DE-He213)s11082-022-04386-0-p DE-627 ger DE-627 rakwb eng 500 620 VZ Le, Vannhu verfasserin aut Saturated confocal fluorescence microscopy with linear polarization modulation 2022 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. Abstract In confocal scanning fluorescence microscopy, the effective modulation transfer function with Gaussian plane wave illumination covers very few high-frequency components, which prohibits further improvement of the spatial resolution. In this study, we propose saturated confocal scanning fluorescence microscopy with linear polarization to achieve super-resolution imaging. In saturated confocal scanning fluorescence microscopy with linear polarization, the effective modulation transfer function in the Fourier domain is extended in comparison with that of Gaussian plane wave illumination. The digital algorithm is adapted to retrieve the super-resolved image from the modulated recordings. The simulation results demonstrated that saturated confocal scanning fluorescence microscopy with linear polarization could be used to increase the resolution in confocal scanning fluorescence microscopy. Confocal fluorescence microscopy (CFM) Super-resolution RL Algorithm Enthalten in Optical and quantum electronics Springer US, 1975 55(2022), 1 vom: 16. Dez. (DE-627)129419540 (DE-600)189950-8 (DE-576)014796139 0306-8919 nnns volume:55 year:2022 number:1 day:16 month:12 https://doi.org/10.1007/s11082-022-04386-0 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-PHY AR 55 2022 1 16 12 |
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10.1007/s11082-022-04386-0 doi (DE-627)OLC2080168460 (DE-He213)s11082-022-04386-0-p DE-627 ger DE-627 rakwb eng 500 620 VZ Le, Vannhu verfasserin aut Saturated confocal fluorescence microscopy with linear polarization modulation 2022 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. Abstract In confocal scanning fluorescence microscopy, the effective modulation transfer function with Gaussian plane wave illumination covers very few high-frequency components, which prohibits further improvement of the spatial resolution. In this study, we propose saturated confocal scanning fluorescence microscopy with linear polarization to achieve super-resolution imaging. In saturated confocal scanning fluorescence microscopy with linear polarization, the effective modulation transfer function in the Fourier domain is extended in comparison with that of Gaussian plane wave illumination. The digital algorithm is adapted to retrieve the super-resolved image from the modulated recordings. The simulation results demonstrated that saturated confocal scanning fluorescence microscopy with linear polarization could be used to increase the resolution in confocal scanning fluorescence microscopy. Confocal fluorescence microscopy (CFM) Super-resolution RL Algorithm Enthalten in Optical and quantum electronics Springer US, 1975 55(2022), 1 vom: 16. Dez. (DE-627)129419540 (DE-600)189950-8 (DE-576)014796139 0306-8919 nnns volume:55 year:2022 number:1 day:16 month:12 https://doi.org/10.1007/s11082-022-04386-0 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-PHY AR 55 2022 1 16 12 |
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Abstract In confocal scanning fluorescence microscopy, the effective modulation transfer function with Gaussian plane wave illumination covers very few high-frequency components, which prohibits further improvement of the spatial resolution. In this study, we propose saturated confocal scanning fluorescence microscopy with linear polarization to achieve super-resolution imaging. In saturated confocal scanning fluorescence microscopy with linear polarization, the effective modulation transfer function in the Fourier domain is extended in comparison with that of Gaussian plane wave illumination. The digital algorithm is adapted to retrieve the super-resolved image from the modulated recordings. The simulation results demonstrated that saturated confocal scanning fluorescence microscopy with linear polarization could be used to increase the resolution in confocal scanning fluorescence microscopy. © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. |
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Abstract In confocal scanning fluorescence microscopy, the effective modulation transfer function with Gaussian plane wave illumination covers very few high-frequency components, which prohibits further improvement of the spatial resolution. In this study, we propose saturated confocal scanning fluorescence microscopy with linear polarization to achieve super-resolution imaging. In saturated confocal scanning fluorescence microscopy with linear polarization, the effective modulation transfer function in the Fourier domain is extended in comparison with that of Gaussian plane wave illumination. The digital algorithm is adapted to retrieve the super-resolved image from the modulated recordings. The simulation results demonstrated that saturated confocal scanning fluorescence microscopy with linear polarization could be used to increase the resolution in confocal scanning fluorescence microscopy. © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. |
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Abstract In confocal scanning fluorescence microscopy, the effective modulation transfer function with Gaussian plane wave illumination covers very few high-frequency components, which prohibits further improvement of the spatial resolution. In this study, we propose saturated confocal scanning fluorescence microscopy with linear polarization to achieve super-resolution imaging. In saturated confocal scanning fluorescence microscopy with linear polarization, the effective modulation transfer function in the Fourier domain is extended in comparison with that of Gaussian plane wave illumination. The digital algorithm is adapted to retrieve the super-resolved image from the modulated recordings. The simulation results demonstrated that saturated confocal scanning fluorescence microscopy with linear polarization could be used to increase the resolution in confocal scanning fluorescence microscopy. © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. |
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Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract In confocal scanning fluorescence microscopy, the effective modulation transfer function with Gaussian plane wave illumination covers very few high-frequency components, which prohibits further improvement of the spatial resolution. In this study, we propose saturated confocal scanning fluorescence microscopy with linear polarization to achieve super-resolution imaging. In saturated confocal scanning fluorescence microscopy with linear polarization, the effective modulation transfer function in the Fourier domain is extended in comparison with that of Gaussian plane wave illumination. The digital algorithm is adapted to retrieve the super-resolved image from the modulated recordings. 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