The impact of agarose immobilization on the activity of lytic Pseudomonas aeruginosa phages combined with chemicals
Abstract The implementation of non-traditional antibacterials is currently one of the most intensively explored areas of modern medical and biological sciences. One of the most promising alternative strategies to combat bacterial infections is the application of lytic phages combined with establishe...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Dorotkiewicz-Jach, Agata [verfasserIn] |
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| Format: |
Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2023 |
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| Schlagwörter: |
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| Anmerkung: |
© The Author(s) 2023 |
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| Übergeordnetes Werk: |
Enthalten in: Applied microbiology and biotechnology - Springer Berlin Heidelberg, 1984, 107(2023), 2-3 vom: 10. Jan., Seite 897-913 |
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| Übergeordnetes Werk: |
volume:107 ; year:2023 ; number:2-3 ; day:10 ; month:01 ; pages:897-913 |
| Links: |
|---|
| DOI / URN: |
10.1007/s00253-022-12349-4 |
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| Katalog-ID: |
OLC2080312464 |
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| 245 | 1 | 0 | |a The impact of agarose immobilization on the activity of lytic Pseudomonas aeruginosa phages combined with chemicals |
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| 520 | |a Abstract The implementation of non-traditional antibacterials is currently one of the most intensively explored areas of modern medical and biological sciences. One of the most promising alternative strategies to combat bacterial infections is the application of lytic phages combined with established and new antibacterials. The presented study investigates the potential of agarose-based biocomposites containing lytic Pseudomonas phages (KT28, KTN4, and LUZ19), cupric ions ($ Cu^{2+} $), strawberry furanone (HDMF), and gentamicin (GE) as antibacterials and anti-virulent compounds for novel wound dressings. Phages (KT28, KTN4, LUZ19, and triple-phage cocktail) alone and in combination with a triple-chemical mixture (Cu + GE + HDMF) when applied as the liquid formulation caused a significant bacterial count reduction and biofilm production inhibition of clinical P. aeruginosa strains. The immobilization in the agarose scaffold significantly impaired the bioavailability and diffusion of phage particles, depending on virion morphology and targeted receptor specificity. The antibacterial potential of chemicals was also reduced by the agarose scaffold. Moreover, the Cu + GE + HDMF mixture impaired the lytic activity of phages depending on viral particles’ susceptibility to cupric ion toxicity. Therefore, three administration types were tested and the optimal turned out to be the one separating antibacterials both physically and temporally. Taken together, the additive effect of phages combined with chemicals makes biocomposite a good solution for designing new wound dressings. Nevertheless, the phage utilization should involve an application of aqueous cocktails directly onto the wound, followed by chemicals immobilized in hydrogel dressings which allow for taking advantage of the antibacterial and anti-virulent effects of all components. Key points • The immobilization in the agarose impairs the bioavailability of phage particles and the Cu + GE + HDMF mixture. • The cupric ions are toxic to phages and are sequestrated on phage particles and agarose matrix. • The elaborated TIME-SHIFT administration effectively separates antibacterials both physically and temporally. | ||
| 650 | 4 | |a Agarose immobilization | |
| 650 | 4 | |a Phages | |
| 650 | 4 | |a Cupric ions | |
| 650 | 4 | |a Gentamicin | |
| 650 | 4 | |a HDMF furanone | |
| 700 | 1 | |a Markwitz, Paweł |0 (orcid)0000-0002-3511-1101 |4 aut | |
| 700 | 1 | |a Rachuna, Jarosław |0 (orcid)0000-0002-0044-5419 |4 aut | |
| 700 | 1 | |a Arabski, Michał |0 (orcid)0000-0002-9712-9286 |4 aut | |
| 700 | 1 | |a Drulis-Kawa, Zuzanna |0 (orcid)0000-0002-4733-4660 |4 aut | |
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10.1007/s00253-022-12349-4 doi (DE-627)OLC2080312464 (DE-He213)s00253-022-12349-4-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid Dorotkiewicz-Jach, Agata verfasserin (orcid)0000-0001-5371-3897 aut The impact of agarose immobilization on the activity of lytic Pseudomonas aeruginosa phages combined with chemicals 2023 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © The Author(s) 2023 Abstract The implementation of non-traditional antibacterials is currently one of the most intensively explored areas of modern medical and biological sciences. One of the most promising alternative strategies to combat bacterial infections is the application of lytic phages combined with established and new antibacterials. The presented study investigates the potential of agarose-based biocomposites containing lytic Pseudomonas phages (KT28, KTN4, and LUZ19), cupric ions ($ Cu^{2+} $), strawberry furanone (HDMF), and gentamicin (GE) as antibacterials and anti-virulent compounds for novel wound dressings. Phages (KT28, KTN4, LUZ19, and triple-phage cocktail) alone and in combination with a triple-chemical mixture (Cu + GE + HDMF) when applied as the liquid formulation caused a significant bacterial count reduction and biofilm production inhibition of clinical P. aeruginosa strains. The immobilization in the agarose scaffold significantly impaired the bioavailability and diffusion of phage particles, depending on virion morphology and targeted receptor specificity. The antibacterial potential of chemicals was also reduced by the agarose scaffold. Moreover, the Cu + GE + HDMF mixture impaired the lytic activity of phages depending on viral particles’ susceptibility to cupric ion toxicity. Therefore, three administration types were tested and the optimal turned out to be the one separating antibacterials both physically and temporally. Taken together, the additive effect of phages combined with chemicals makes biocomposite a good solution for designing new wound dressings. Nevertheless, the phage utilization should involve an application of aqueous cocktails directly onto the wound, followed by chemicals immobilized in hydrogel dressings which allow for taking advantage of the antibacterial and anti-virulent effects of all components. Key points • The immobilization in the agarose impairs the bioavailability of phage particles and the Cu + GE + HDMF mixture. • The cupric ions are toxic to phages and are sequestrated on phage particles and agarose matrix. • The elaborated TIME-SHIFT administration effectively separates antibacterials both physically and temporally. Agarose immobilization Phages Cupric ions Gentamicin HDMF furanone Markwitz, Paweł (orcid)0000-0002-3511-1101 aut Rachuna, Jarosław (orcid)0000-0002-0044-5419 aut Arabski, Michał (orcid)0000-0002-9712-9286 aut Drulis-Kawa, Zuzanna (orcid)0000-0002-4733-4660 aut Enthalten in Applied microbiology and biotechnology Springer Berlin Heidelberg, 1984 107(2023), 2-3 vom: 10. Jan., Seite 897-913 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:107 year:2023 number:2-3 day:10 month:01 pages:897-913 https://doi.org/10.1007/s00253-022-12349-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_267 GBV_ILN_2018 GBV_ILN_4277 AR 107 2023 2-3 10 01 897-913 |
| spelling |
10.1007/s00253-022-12349-4 doi (DE-627)OLC2080312464 (DE-He213)s00253-022-12349-4-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid Dorotkiewicz-Jach, Agata verfasserin (orcid)0000-0001-5371-3897 aut The impact of agarose immobilization on the activity of lytic Pseudomonas aeruginosa phages combined with chemicals 2023 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © The Author(s) 2023 Abstract The implementation of non-traditional antibacterials is currently one of the most intensively explored areas of modern medical and biological sciences. One of the most promising alternative strategies to combat bacterial infections is the application of lytic phages combined with established and new antibacterials. The presented study investigates the potential of agarose-based biocomposites containing lytic Pseudomonas phages (KT28, KTN4, and LUZ19), cupric ions ($ Cu^{2+} $), strawberry furanone (HDMF), and gentamicin (GE) as antibacterials and anti-virulent compounds for novel wound dressings. Phages (KT28, KTN4, LUZ19, and triple-phage cocktail) alone and in combination with a triple-chemical mixture (Cu + GE + HDMF) when applied as the liquid formulation caused a significant bacterial count reduction and biofilm production inhibition of clinical P. aeruginosa strains. The immobilization in the agarose scaffold significantly impaired the bioavailability and diffusion of phage particles, depending on virion morphology and targeted receptor specificity. The antibacterial potential of chemicals was also reduced by the agarose scaffold. Moreover, the Cu + GE + HDMF mixture impaired the lytic activity of phages depending on viral particles’ susceptibility to cupric ion toxicity. Therefore, three administration types were tested and the optimal turned out to be the one separating antibacterials both physically and temporally. Taken together, the additive effect of phages combined with chemicals makes biocomposite a good solution for designing new wound dressings. Nevertheless, the phage utilization should involve an application of aqueous cocktails directly onto the wound, followed by chemicals immobilized in hydrogel dressings which allow for taking advantage of the antibacterial and anti-virulent effects of all components. Key points • The immobilization in the agarose impairs the bioavailability of phage particles and the Cu + GE + HDMF mixture. • The cupric ions are toxic to phages and are sequestrated on phage particles and agarose matrix. • The elaborated TIME-SHIFT administration effectively separates antibacterials both physically and temporally. Agarose immobilization Phages Cupric ions Gentamicin HDMF furanone Markwitz, Paweł (orcid)0000-0002-3511-1101 aut Rachuna, Jarosław (orcid)0000-0002-0044-5419 aut Arabski, Michał (orcid)0000-0002-9712-9286 aut Drulis-Kawa, Zuzanna (orcid)0000-0002-4733-4660 aut Enthalten in Applied microbiology and biotechnology Springer Berlin Heidelberg, 1984 107(2023), 2-3 vom: 10. Jan., Seite 897-913 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:107 year:2023 number:2-3 day:10 month:01 pages:897-913 https://doi.org/10.1007/s00253-022-12349-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_267 GBV_ILN_2018 GBV_ILN_4277 AR 107 2023 2-3 10 01 897-913 |
| allfields_unstemmed |
10.1007/s00253-022-12349-4 doi (DE-627)OLC2080312464 (DE-He213)s00253-022-12349-4-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid Dorotkiewicz-Jach, Agata verfasserin (orcid)0000-0001-5371-3897 aut The impact of agarose immobilization on the activity of lytic Pseudomonas aeruginosa phages combined with chemicals 2023 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © The Author(s) 2023 Abstract The implementation of non-traditional antibacterials is currently one of the most intensively explored areas of modern medical and biological sciences. One of the most promising alternative strategies to combat bacterial infections is the application of lytic phages combined with established and new antibacterials. The presented study investigates the potential of agarose-based biocomposites containing lytic Pseudomonas phages (KT28, KTN4, and LUZ19), cupric ions ($ Cu^{2+} $), strawberry furanone (HDMF), and gentamicin (GE) as antibacterials and anti-virulent compounds for novel wound dressings. Phages (KT28, KTN4, LUZ19, and triple-phage cocktail) alone and in combination with a triple-chemical mixture (Cu + GE + HDMF) when applied as the liquid formulation caused a significant bacterial count reduction and biofilm production inhibition of clinical P. aeruginosa strains. The immobilization in the agarose scaffold significantly impaired the bioavailability and diffusion of phage particles, depending on virion morphology and targeted receptor specificity. The antibacterial potential of chemicals was also reduced by the agarose scaffold. Moreover, the Cu + GE + HDMF mixture impaired the lytic activity of phages depending on viral particles’ susceptibility to cupric ion toxicity. Therefore, three administration types were tested and the optimal turned out to be the one separating antibacterials both physically and temporally. Taken together, the additive effect of phages combined with chemicals makes biocomposite a good solution for designing new wound dressings. Nevertheless, the phage utilization should involve an application of aqueous cocktails directly onto the wound, followed by chemicals immobilized in hydrogel dressings which allow for taking advantage of the antibacterial and anti-virulent effects of all components. Key points • The immobilization in the agarose impairs the bioavailability of phage particles and the Cu + GE + HDMF mixture. • The cupric ions are toxic to phages and are sequestrated on phage particles and agarose matrix. • The elaborated TIME-SHIFT administration effectively separates antibacterials both physically and temporally. Agarose immobilization Phages Cupric ions Gentamicin HDMF furanone Markwitz, Paweł (orcid)0000-0002-3511-1101 aut Rachuna, Jarosław (orcid)0000-0002-0044-5419 aut Arabski, Michał (orcid)0000-0002-9712-9286 aut Drulis-Kawa, Zuzanna (orcid)0000-0002-4733-4660 aut Enthalten in Applied microbiology and biotechnology Springer Berlin Heidelberg, 1984 107(2023), 2-3 vom: 10. Jan., Seite 897-913 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:107 year:2023 number:2-3 day:10 month:01 pages:897-913 https://doi.org/10.1007/s00253-022-12349-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_267 GBV_ILN_2018 GBV_ILN_4277 AR 107 2023 2-3 10 01 897-913 |
| allfieldsGer |
10.1007/s00253-022-12349-4 doi (DE-627)OLC2080312464 (DE-He213)s00253-022-12349-4-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid Dorotkiewicz-Jach, Agata verfasserin (orcid)0000-0001-5371-3897 aut The impact of agarose immobilization on the activity of lytic Pseudomonas aeruginosa phages combined with chemicals 2023 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © The Author(s) 2023 Abstract The implementation of non-traditional antibacterials is currently one of the most intensively explored areas of modern medical and biological sciences. One of the most promising alternative strategies to combat bacterial infections is the application of lytic phages combined with established and new antibacterials. The presented study investigates the potential of agarose-based biocomposites containing lytic Pseudomonas phages (KT28, KTN4, and LUZ19), cupric ions ($ Cu^{2+} $), strawberry furanone (HDMF), and gentamicin (GE) as antibacterials and anti-virulent compounds for novel wound dressings. Phages (KT28, KTN4, LUZ19, and triple-phage cocktail) alone and in combination with a triple-chemical mixture (Cu + GE + HDMF) when applied as the liquid formulation caused a significant bacterial count reduction and biofilm production inhibition of clinical P. aeruginosa strains. The immobilization in the agarose scaffold significantly impaired the bioavailability and diffusion of phage particles, depending on virion morphology and targeted receptor specificity. The antibacterial potential of chemicals was also reduced by the agarose scaffold. Moreover, the Cu + GE + HDMF mixture impaired the lytic activity of phages depending on viral particles’ susceptibility to cupric ion toxicity. Therefore, three administration types were tested and the optimal turned out to be the one separating antibacterials both physically and temporally. Taken together, the additive effect of phages combined with chemicals makes biocomposite a good solution for designing new wound dressings. Nevertheless, the phage utilization should involve an application of aqueous cocktails directly onto the wound, followed by chemicals immobilized in hydrogel dressings which allow for taking advantage of the antibacterial and anti-virulent effects of all components. Key points • The immobilization in the agarose impairs the bioavailability of phage particles and the Cu + GE + HDMF mixture. • The cupric ions are toxic to phages and are sequestrated on phage particles and agarose matrix. • The elaborated TIME-SHIFT administration effectively separates antibacterials both physically and temporally. Agarose immobilization Phages Cupric ions Gentamicin HDMF furanone Markwitz, Paweł (orcid)0000-0002-3511-1101 aut Rachuna, Jarosław (orcid)0000-0002-0044-5419 aut Arabski, Michał (orcid)0000-0002-9712-9286 aut Drulis-Kawa, Zuzanna (orcid)0000-0002-4733-4660 aut Enthalten in Applied microbiology and biotechnology Springer Berlin Heidelberg, 1984 107(2023), 2-3 vom: 10. Jan., Seite 897-913 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:107 year:2023 number:2-3 day:10 month:01 pages:897-913 https://doi.org/10.1007/s00253-022-12349-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_267 GBV_ILN_2018 GBV_ILN_4277 AR 107 2023 2-3 10 01 897-913 |
| allfieldsSound |
10.1007/s00253-022-12349-4 doi (DE-627)OLC2080312464 (DE-He213)s00253-022-12349-4-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid Dorotkiewicz-Jach, Agata verfasserin (orcid)0000-0001-5371-3897 aut The impact of agarose immobilization on the activity of lytic Pseudomonas aeruginosa phages combined with chemicals 2023 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © The Author(s) 2023 Abstract The implementation of non-traditional antibacterials is currently one of the most intensively explored areas of modern medical and biological sciences. One of the most promising alternative strategies to combat bacterial infections is the application of lytic phages combined with established and new antibacterials. The presented study investigates the potential of agarose-based biocomposites containing lytic Pseudomonas phages (KT28, KTN4, and LUZ19), cupric ions ($ Cu^{2+} $), strawberry furanone (HDMF), and gentamicin (GE) as antibacterials and anti-virulent compounds for novel wound dressings. Phages (KT28, KTN4, LUZ19, and triple-phage cocktail) alone and in combination with a triple-chemical mixture (Cu + GE + HDMF) when applied as the liquid formulation caused a significant bacterial count reduction and biofilm production inhibition of clinical P. aeruginosa strains. The immobilization in the agarose scaffold significantly impaired the bioavailability and diffusion of phage particles, depending on virion morphology and targeted receptor specificity. The antibacterial potential of chemicals was also reduced by the agarose scaffold. Moreover, the Cu + GE + HDMF mixture impaired the lytic activity of phages depending on viral particles’ susceptibility to cupric ion toxicity. Therefore, three administration types were tested and the optimal turned out to be the one separating antibacterials both physically and temporally. Taken together, the additive effect of phages combined with chemicals makes biocomposite a good solution for designing new wound dressings. Nevertheless, the phage utilization should involve an application of aqueous cocktails directly onto the wound, followed by chemicals immobilized in hydrogel dressings which allow for taking advantage of the antibacterial and anti-virulent effects of all components. Key points • The immobilization in the agarose impairs the bioavailability of phage particles and the Cu + GE + HDMF mixture. • The cupric ions are toxic to phages and are sequestrated on phage particles and agarose matrix. • The elaborated TIME-SHIFT administration effectively separates antibacterials both physically and temporally. Agarose immobilization Phages Cupric ions Gentamicin HDMF furanone Markwitz, Paweł (orcid)0000-0002-3511-1101 aut Rachuna, Jarosław (orcid)0000-0002-0044-5419 aut Arabski, Michał (orcid)0000-0002-9712-9286 aut Drulis-Kawa, Zuzanna (orcid)0000-0002-4733-4660 aut Enthalten in Applied microbiology and biotechnology Springer Berlin Heidelberg, 1984 107(2023), 2-3 vom: 10. Jan., Seite 897-913 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:107 year:2023 number:2-3 day:10 month:01 pages:897-913 https://doi.org/10.1007/s00253-022-12349-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_267 GBV_ILN_2018 GBV_ILN_4277 AR 107 2023 2-3 10 01 897-913 |
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Enthalten in Applied microbiology and biotechnology 107(2023), 2-3 vom: 10. Jan., Seite 897-913 volume:107 year:2023 number:2-3 day:10 month:01 pages:897-913 |
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the impact of agarose immobilization on the activity of lytic pseudomonas aeruginosa phages combined with chemicals |
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The impact of agarose immobilization on the activity of lytic Pseudomonas aeruginosa phages combined with chemicals |
| abstract |
Abstract The implementation of non-traditional antibacterials is currently one of the most intensively explored areas of modern medical and biological sciences. One of the most promising alternative strategies to combat bacterial infections is the application of lytic phages combined with established and new antibacterials. The presented study investigates the potential of agarose-based biocomposites containing lytic Pseudomonas phages (KT28, KTN4, and LUZ19), cupric ions ($ Cu^{2+} $), strawberry furanone (HDMF), and gentamicin (GE) as antibacterials and anti-virulent compounds for novel wound dressings. Phages (KT28, KTN4, LUZ19, and triple-phage cocktail) alone and in combination with a triple-chemical mixture (Cu + GE + HDMF) when applied as the liquid formulation caused a significant bacterial count reduction and biofilm production inhibition of clinical P. aeruginosa strains. The immobilization in the agarose scaffold significantly impaired the bioavailability and diffusion of phage particles, depending on virion morphology and targeted receptor specificity. The antibacterial potential of chemicals was also reduced by the agarose scaffold. Moreover, the Cu + GE + HDMF mixture impaired the lytic activity of phages depending on viral particles’ susceptibility to cupric ion toxicity. Therefore, three administration types were tested and the optimal turned out to be the one separating antibacterials both physically and temporally. Taken together, the additive effect of phages combined with chemicals makes biocomposite a good solution for designing new wound dressings. Nevertheless, the phage utilization should involve an application of aqueous cocktails directly onto the wound, followed by chemicals immobilized in hydrogel dressings which allow for taking advantage of the antibacterial and anti-virulent effects of all components. Key points • The immobilization in the agarose impairs the bioavailability of phage particles and the Cu + GE + HDMF mixture. • The cupric ions are toxic to phages and are sequestrated on phage particles and agarose matrix. • The elaborated TIME-SHIFT administration effectively separates antibacterials both physically and temporally. © The Author(s) 2023 |
| abstractGer |
Abstract The implementation of non-traditional antibacterials is currently one of the most intensively explored areas of modern medical and biological sciences. One of the most promising alternative strategies to combat bacterial infections is the application of lytic phages combined with established and new antibacterials. The presented study investigates the potential of agarose-based biocomposites containing lytic Pseudomonas phages (KT28, KTN4, and LUZ19), cupric ions ($ Cu^{2+} $), strawberry furanone (HDMF), and gentamicin (GE) as antibacterials and anti-virulent compounds for novel wound dressings. Phages (KT28, KTN4, LUZ19, and triple-phage cocktail) alone and in combination with a triple-chemical mixture (Cu + GE + HDMF) when applied as the liquid formulation caused a significant bacterial count reduction and biofilm production inhibition of clinical P. aeruginosa strains. The immobilization in the agarose scaffold significantly impaired the bioavailability and diffusion of phage particles, depending on virion morphology and targeted receptor specificity. The antibacterial potential of chemicals was also reduced by the agarose scaffold. Moreover, the Cu + GE + HDMF mixture impaired the lytic activity of phages depending on viral particles’ susceptibility to cupric ion toxicity. Therefore, three administration types were tested and the optimal turned out to be the one separating antibacterials both physically and temporally. Taken together, the additive effect of phages combined with chemicals makes biocomposite a good solution for designing new wound dressings. Nevertheless, the phage utilization should involve an application of aqueous cocktails directly onto the wound, followed by chemicals immobilized in hydrogel dressings which allow for taking advantage of the antibacterial and anti-virulent effects of all components. Key points • The immobilization in the agarose impairs the bioavailability of phage particles and the Cu + GE + HDMF mixture. • The cupric ions are toxic to phages and are sequestrated on phage particles and agarose matrix. • The elaborated TIME-SHIFT administration effectively separates antibacterials both physically and temporally. © The Author(s) 2023 |
| abstract_unstemmed |
Abstract The implementation of non-traditional antibacterials is currently one of the most intensively explored areas of modern medical and biological sciences. One of the most promising alternative strategies to combat bacterial infections is the application of lytic phages combined with established and new antibacterials. The presented study investigates the potential of agarose-based biocomposites containing lytic Pseudomonas phages (KT28, KTN4, and LUZ19), cupric ions ($ Cu^{2+} $), strawberry furanone (HDMF), and gentamicin (GE) as antibacterials and anti-virulent compounds for novel wound dressings. Phages (KT28, KTN4, LUZ19, and triple-phage cocktail) alone and in combination with a triple-chemical mixture (Cu + GE + HDMF) when applied as the liquid formulation caused a significant bacterial count reduction and biofilm production inhibition of clinical P. aeruginosa strains. The immobilization in the agarose scaffold significantly impaired the bioavailability and diffusion of phage particles, depending on virion morphology and targeted receptor specificity. The antibacterial potential of chemicals was also reduced by the agarose scaffold. Moreover, the Cu + GE + HDMF mixture impaired the lytic activity of phages depending on viral particles’ susceptibility to cupric ion toxicity. Therefore, three administration types were tested and the optimal turned out to be the one separating antibacterials both physically and temporally. Taken together, the additive effect of phages combined with chemicals makes biocomposite a good solution for designing new wound dressings. Nevertheless, the phage utilization should involve an application of aqueous cocktails directly onto the wound, followed by chemicals immobilized in hydrogel dressings which allow for taking advantage of the antibacterial and anti-virulent effects of all components. Key points • The immobilization in the agarose impairs the bioavailability of phage particles and the Cu + GE + HDMF mixture. • The cupric ions are toxic to phages and are sequestrated on phage particles and agarose matrix. • The elaborated TIME-SHIFT administration effectively separates antibacterials both physically and temporally. © The Author(s) 2023 |
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The antibacterial potential of chemicals was also reduced by the agarose scaffold. Moreover, the Cu + GE + HDMF mixture impaired the lytic activity of phages depending on viral particles’ susceptibility to cupric ion toxicity. Therefore, three administration types were tested and the optimal turned out to be the one separating antibacterials both physically and temporally. Taken together, the additive effect of phages combined with chemicals makes biocomposite a good solution for designing new wound dressings. Nevertheless, the phage utilization should involve an application of aqueous cocktails directly onto the wound, followed by chemicals immobilized in hydrogel dressings which allow for taking advantage of the antibacterial and anti-virulent effects of all components. Key points • The immobilization in the agarose impairs the bioavailability of phage particles and the Cu + GE + HDMF mixture. • The cupric ions are toxic to phages and are sequestrated on phage particles and agarose matrix. • The elaborated TIME-SHIFT administration effectively separates antibacterials both physically and temporally.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Agarose immobilization</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Phages</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Cupric ions</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Gentamicin</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">HDMF furanone</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Markwitz, Paweł</subfield><subfield code="0">(orcid)0000-0002-3511-1101</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Rachuna, Jarosław</subfield><subfield code="0">(orcid)0000-0002-0044-5419</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Arabski, Michał</subfield><subfield code="0">(orcid)0000-0002-9712-9286</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Drulis-Kawa, Zuzanna</subfield><subfield code="0">(orcid)0000-0002-4733-4660</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Applied microbiology and biotechnology</subfield><subfield code="d">Springer Berlin Heidelberg, 1984</subfield><subfield code="g">107(2023), 2-3 vom: 10. Jan., Seite 897-913</subfield><subfield code="w">(DE-627)129942634</subfield><subfield code="w">(DE-600)392453-1</subfield><subfield code="w">(DE-576)015507750</subfield><subfield code="x">0175-7598</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:107</subfield><subfield code="g">year:2023</subfield><subfield code="g">number:2-3</subfield><subfield code="g">day:10</subfield><subfield code="g">month:01</subfield><subfield code="g">pages:897-913</subfield></datafield><datafield tag="856" ind1="4" ind2="1"><subfield code="u">https://doi.org/10.1007/s00253-022-12349-4</subfield><subfield code="z">lizenzpflichtig</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_OLC</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">FID-BIODIV</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-TEC</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-CHE</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-PHA</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-DE-84</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_267</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2018</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4277</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">107</subfield><subfield code="j">2023</subfield><subfield code="e">2-3</subfield><subfield code="b">10</subfield><subfield code="c">01</subfield><subfield code="h">897-913</subfield></datafield></record></collection>
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