Minimizing base loss and internal fragmentation in collisionally activated dissociation of multiply deprotonated RNA
Abstract In recent years, new classes of nonprotein-coding ribonucleic acids (ncRNAs) with important cellular functions have been discovered. Of particular interest for biomolecular research and pharmaceutical developments are small ncRNAs that are involved in gene regulation, such as small interfer...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Taucher, Monika [verfasserIn] |
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| Format: |
Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2010 |
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| Schlagwörter: |
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| Anmerkung: |
© American Society for Mass Spectrometry 2010 |
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| Übergeordnetes Werk: |
Enthalten in: Journal of the American Society for Mass Spectrometry - Springer-Verlag, 1990, 21(2010), 2 vom: Feb., Seite 278-285 |
|---|---|
| Übergeordnetes Werk: |
volume:21 ; year:2010 ; number:2 ; month:02 ; pages:278-285 |
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| DOI / URN: |
10.1016/j.jasms.2009.10.010 |
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| 520 | |a Abstract In recent years, new classes of nonprotein-coding ribonucleic acids (ncRNAs) with important cellular functions have been discovered. Of particular interest for biomolecular research and pharmaceutical developments are small ncRNAs that are involved in gene regulation, such as small interfering RNAs (21–28 nt), pre-microRNAs (70–80 nt), or riboswitches (34–200 nt). De novo sequencing of RNA by top-down mass spectrometry has so far been limited to RNA consisting of up to ∼20 nt. We report here complete sequence coverage for 34 nt RNA (10.9 kDa), along with 30 out of 32 possible complementary ion pairs from collisionally activated dissociation (CAD) experiments. The key to minimizing undesired base loss and internal fragmentation is to minimize the internal energy of fragment ions from primary backbone cleavage. This can be achieved by collisional cooling of primary fragment ions and selection of precursor ions of relatively low negative net charge (about −0.2/nt). | ||
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10.1016/j.jasms.2009.10.010 doi (DE-627)OLC2097686915 (DE-He213)j.jasms.2009.10.010-p DE-627 ger DE-627 rakwb eng 530 VZ 11 ssgn Taucher, Monika verfasserin aut Minimizing base loss and internal fragmentation in collisionally activated dissociation of multiply deprotonated RNA 2010 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © American Society for Mass Spectrometry 2010 Abstract In recent years, new classes of nonprotein-coding ribonucleic acids (ncRNAs) with important cellular functions have been discovered. Of particular interest for biomolecular research and pharmaceutical developments are small ncRNAs that are involved in gene regulation, such as small interfering RNAs (21–28 nt), pre-microRNAs (70–80 nt), or riboswitches (34–200 nt). De novo sequencing of RNA by top-down mass spectrometry has so far been limited to RNA consisting of up to ∼20 nt. We report here complete sequence coverage for 34 nt RNA (10.9 kDa), along with 30 out of 32 possible complementary ion pairs from collisionally activated dissociation (CAD) experiments. The key to minimizing undesired base loss and internal fragmentation is to minimize the internal energy of fragment ions from primary backbone cleavage. This can be achieved by collisional cooling of primary fragment ions and selection of precursor ions of relatively low negative net charge (about −0.2/nt). Collisionally Activate Dissociation Backbone Cleavage Base Loss Collisional Cool Collisionally Activate Dissociation Spectrum Rieder, Ulrike aut Breuker, Kathrin aut Enthalten in Journal of the American Society for Mass Spectrometry Springer-Verlag, 1990 21(2010), 2 vom: Feb., Seite 278-285 (DE-627)130977357 (DE-600)1073671-2 (DE-576)277732093 1044-0305 nnns volume:21 year:2010 number:2 month:02 pages:278-285 https://doi.org/10.1016/j.jasms.2009.10.010 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-PHY SSG-OLC-CHE GBV_ILN_70 GBV_ILN_2004 GBV_ILN_4012 GBV_ILN_4125 GBV_ILN_4307 AR 21 2010 2 02 278-285 |
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10.1016/j.jasms.2009.10.010 doi (DE-627)OLC2097686915 (DE-He213)j.jasms.2009.10.010-p DE-627 ger DE-627 rakwb eng 530 VZ 11 ssgn Taucher, Monika verfasserin aut Minimizing base loss and internal fragmentation in collisionally activated dissociation of multiply deprotonated RNA 2010 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © American Society for Mass Spectrometry 2010 Abstract In recent years, new classes of nonprotein-coding ribonucleic acids (ncRNAs) with important cellular functions have been discovered. Of particular interest for biomolecular research and pharmaceutical developments are small ncRNAs that are involved in gene regulation, such as small interfering RNAs (21–28 nt), pre-microRNAs (70–80 nt), or riboswitches (34–200 nt). De novo sequencing of RNA by top-down mass spectrometry has so far been limited to RNA consisting of up to ∼20 nt. We report here complete sequence coverage for 34 nt RNA (10.9 kDa), along with 30 out of 32 possible complementary ion pairs from collisionally activated dissociation (CAD) experiments. The key to minimizing undesired base loss and internal fragmentation is to minimize the internal energy of fragment ions from primary backbone cleavage. This can be achieved by collisional cooling of primary fragment ions and selection of precursor ions of relatively low negative net charge (about −0.2/nt). Collisionally Activate Dissociation Backbone Cleavage Base Loss Collisional Cool Collisionally Activate Dissociation Spectrum Rieder, Ulrike aut Breuker, Kathrin aut Enthalten in Journal of the American Society for Mass Spectrometry Springer-Verlag, 1990 21(2010), 2 vom: Feb., Seite 278-285 (DE-627)130977357 (DE-600)1073671-2 (DE-576)277732093 1044-0305 nnns volume:21 year:2010 number:2 month:02 pages:278-285 https://doi.org/10.1016/j.jasms.2009.10.010 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-PHY SSG-OLC-CHE GBV_ILN_70 GBV_ILN_2004 GBV_ILN_4012 GBV_ILN_4125 GBV_ILN_4307 AR 21 2010 2 02 278-285 |
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10.1016/j.jasms.2009.10.010 doi (DE-627)OLC2097686915 (DE-He213)j.jasms.2009.10.010-p DE-627 ger DE-627 rakwb eng 530 VZ 11 ssgn Taucher, Monika verfasserin aut Minimizing base loss and internal fragmentation in collisionally activated dissociation of multiply deprotonated RNA 2010 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © American Society for Mass Spectrometry 2010 Abstract In recent years, new classes of nonprotein-coding ribonucleic acids (ncRNAs) with important cellular functions have been discovered. Of particular interest for biomolecular research and pharmaceutical developments are small ncRNAs that are involved in gene regulation, such as small interfering RNAs (21–28 nt), pre-microRNAs (70–80 nt), or riboswitches (34–200 nt). De novo sequencing of RNA by top-down mass spectrometry has so far been limited to RNA consisting of up to ∼20 nt. We report here complete sequence coverage for 34 nt RNA (10.9 kDa), along with 30 out of 32 possible complementary ion pairs from collisionally activated dissociation (CAD) experiments. The key to minimizing undesired base loss and internal fragmentation is to minimize the internal energy of fragment ions from primary backbone cleavage. This can be achieved by collisional cooling of primary fragment ions and selection of precursor ions of relatively low negative net charge (about −0.2/nt). Collisionally Activate Dissociation Backbone Cleavage Base Loss Collisional Cool Collisionally Activate Dissociation Spectrum Rieder, Ulrike aut Breuker, Kathrin aut Enthalten in Journal of the American Society for Mass Spectrometry Springer-Verlag, 1990 21(2010), 2 vom: Feb., Seite 278-285 (DE-627)130977357 (DE-600)1073671-2 (DE-576)277732093 1044-0305 nnns volume:21 year:2010 number:2 month:02 pages:278-285 https://doi.org/10.1016/j.jasms.2009.10.010 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-PHY SSG-OLC-CHE GBV_ILN_70 GBV_ILN_2004 GBV_ILN_4012 GBV_ILN_4125 GBV_ILN_4307 AR 21 2010 2 02 278-285 |
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10.1016/j.jasms.2009.10.010 doi (DE-627)OLC2097686915 (DE-He213)j.jasms.2009.10.010-p DE-627 ger DE-627 rakwb eng 530 VZ 11 ssgn Taucher, Monika verfasserin aut Minimizing base loss and internal fragmentation in collisionally activated dissociation of multiply deprotonated RNA 2010 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © American Society for Mass Spectrometry 2010 Abstract In recent years, new classes of nonprotein-coding ribonucleic acids (ncRNAs) with important cellular functions have been discovered. Of particular interest for biomolecular research and pharmaceutical developments are small ncRNAs that are involved in gene regulation, such as small interfering RNAs (21–28 nt), pre-microRNAs (70–80 nt), or riboswitches (34–200 nt). De novo sequencing of RNA by top-down mass spectrometry has so far been limited to RNA consisting of up to ∼20 nt. We report here complete sequence coverage for 34 nt RNA (10.9 kDa), along with 30 out of 32 possible complementary ion pairs from collisionally activated dissociation (CAD) experiments. The key to minimizing undesired base loss and internal fragmentation is to minimize the internal energy of fragment ions from primary backbone cleavage. This can be achieved by collisional cooling of primary fragment ions and selection of precursor ions of relatively low negative net charge (about −0.2/nt). Collisionally Activate Dissociation Backbone Cleavage Base Loss Collisional Cool Collisionally Activate Dissociation Spectrum Rieder, Ulrike aut Breuker, Kathrin aut Enthalten in Journal of the American Society for Mass Spectrometry Springer-Verlag, 1990 21(2010), 2 vom: Feb., Seite 278-285 (DE-627)130977357 (DE-600)1073671-2 (DE-576)277732093 1044-0305 nnns volume:21 year:2010 number:2 month:02 pages:278-285 https://doi.org/10.1016/j.jasms.2009.10.010 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-PHY SSG-OLC-CHE GBV_ILN_70 GBV_ILN_2004 GBV_ILN_4012 GBV_ILN_4125 GBV_ILN_4307 AR 21 2010 2 02 278-285 |
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Minimizing base loss and internal fragmentation in collisionally activated dissociation of multiply deprotonated RNA |
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Abstract In recent years, new classes of nonprotein-coding ribonucleic acids (ncRNAs) with important cellular functions have been discovered. Of particular interest for biomolecular research and pharmaceutical developments are small ncRNAs that are involved in gene regulation, such as small interfering RNAs (21–28 nt), pre-microRNAs (70–80 nt), or riboswitches (34–200 nt). De novo sequencing of RNA by top-down mass spectrometry has so far been limited to RNA consisting of up to ∼20 nt. We report here complete sequence coverage for 34 nt RNA (10.9 kDa), along with 30 out of 32 possible complementary ion pairs from collisionally activated dissociation (CAD) experiments. The key to minimizing undesired base loss and internal fragmentation is to minimize the internal energy of fragment ions from primary backbone cleavage. This can be achieved by collisional cooling of primary fragment ions and selection of precursor ions of relatively low negative net charge (about −0.2/nt). © American Society for Mass Spectrometry 2010 |
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Abstract In recent years, new classes of nonprotein-coding ribonucleic acids (ncRNAs) with important cellular functions have been discovered. Of particular interest for biomolecular research and pharmaceutical developments are small ncRNAs that are involved in gene regulation, such as small interfering RNAs (21–28 nt), pre-microRNAs (70–80 nt), or riboswitches (34–200 nt). De novo sequencing of RNA by top-down mass spectrometry has so far been limited to RNA consisting of up to ∼20 nt. We report here complete sequence coverage for 34 nt RNA (10.9 kDa), along with 30 out of 32 possible complementary ion pairs from collisionally activated dissociation (CAD) experiments. The key to minimizing undesired base loss and internal fragmentation is to minimize the internal energy of fragment ions from primary backbone cleavage. This can be achieved by collisional cooling of primary fragment ions and selection of precursor ions of relatively low negative net charge (about −0.2/nt). © American Society for Mass Spectrometry 2010 |
| abstract_unstemmed |
Abstract In recent years, new classes of nonprotein-coding ribonucleic acids (ncRNAs) with important cellular functions have been discovered. Of particular interest for biomolecular research and pharmaceutical developments are small ncRNAs that are involved in gene regulation, such as small interfering RNAs (21–28 nt), pre-microRNAs (70–80 nt), or riboswitches (34–200 nt). De novo sequencing of RNA by top-down mass spectrometry has so far been limited to RNA consisting of up to ∼20 nt. We report here complete sequence coverage for 34 nt RNA (10.9 kDa), along with 30 out of 32 possible complementary ion pairs from collisionally activated dissociation (CAD) experiments. The key to minimizing undesired base loss and internal fragmentation is to minimize the internal energy of fragment ions from primary backbone cleavage. This can be achieved by collisional cooling of primary fragment ions and selection of precursor ions of relatively low negative net charge (about −0.2/nt). © American Society for Mass Spectrometry 2010 |
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| title_short |
Minimizing base loss and internal fragmentation in collisionally activated dissociation of multiply deprotonated RNA |
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https://doi.org/10.1016/j.jasms.2009.10.010 |
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Rieder, Ulrike Breuker, Kathrin |
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10.1016/j.jasms.2009.10.010 |
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2024-07-03T14:18:12.987Z |
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