Analysis of selective androgen receptor modulators by gas chromatography-microchip atmospheric pressure photoionization-mass spectrometry
Abstract A gas chromatography-microchip atmospheric pressure photoionization-mass spectrometric (GC-μAPPI-MS) method was developed and used for the analysis of three 2-quinolinone-derived selective androgen receptor modulators (SARMs). SARMs were analyzed from spiked urine samples, which were hydrol...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Luosujärvi, Laura [verfasserIn] |
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| Format: |
Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2010 |
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| Schlagwörter: |
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| Anmerkung: |
© American Society for Mass Spectrometry 2010 |
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| Übergeordnetes Werk: |
Enthalten in: Journal of the American Society for Mass Spectrometry - Springer-Verlag, 1990, 21(2010), 2 vom: Feb., Seite 310-316 |
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| Übergeordnetes Werk: |
volume:21 ; year:2010 ; number:2 ; month:02 ; pages:310-316 |
| Links: |
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| DOI / URN: |
10.1016/j.jasms.2009.10.019 |
|---|
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| 520 | |a Abstract A gas chromatography-microchip atmospheric pressure photoionization-mass spectrometric (GC-μAPPI-MS) method was developed and used for the analysis of three 2-quinolinone-derived selective androgen receptor modulators (SARMs). SARMs were analyzed from spiked urine samples, which were hydrolyzed and derivatized with N-methyl-N-(trimethylsilyl)trifluoroacetamide before analysis. Trimethylsilyl derivatives of SARMs formed both radical cations ($ M^{+•} $) and protonated molecules ([M + H]+) in photoionization. Better signal-to-noise ratios (S/N) were obtained in MS/MS analysis using the $ M^{+•} $ ions as precursor ions than using the [M + H]+ ions, and therefore the $ M^{+•} $ ions were selected for the precursor ions in selected reaction monitoring (SRM) analysis. Limits of detection (LODs) with the method ranged from 0.01 to 1 ng/mL, which correspond to instrumental LODs of 0.2–20 pg. Limits of quantitation ranged from 0.03 to 3 ng/mL. The mass spectrometric response to the analytes was linear (R ≥ 0.995) from the LOQ concentration level up to 100 ng/mL concentration, and intra-day repeatabilities were 5%–9%. In addition to the GC-μAPPI-MS study, the proof-of-principle of gas chromatography-microchip atmospheric pressure chemical ionization-Orbitrap MS (GC-μAPCI-Orbitrap MS) was demonstrated. | ||
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| 700 | 1 | |a Ketola, Raimo A. |4 aut | |
| 700 | 1 | |a Kostiainen, Risto |4 aut | |
| 700 | 1 | |a Kotiaho, Tapio |4 aut | |
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10.1016/j.jasms.2009.10.019 doi (DE-627)OLC2097687016 (DE-He213)j.jasms.2009.10.019-p DE-627 ger DE-627 rakwb eng 530 VZ 11 ssgn Luosujärvi, Laura verfasserin aut Analysis of selective androgen receptor modulators by gas chromatography-microchip atmospheric pressure photoionization-mass spectrometry 2010 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © American Society for Mass Spectrometry 2010 Abstract A gas chromatography-microchip atmospheric pressure photoionization-mass spectrometric (GC-μAPPI-MS) method was developed and used for the analysis of three 2-quinolinone-derived selective androgen receptor modulators (SARMs). SARMs were analyzed from spiked urine samples, which were hydrolyzed and derivatized with N-methyl-N-(trimethylsilyl)trifluoroacetamide before analysis. Trimethylsilyl derivatives of SARMs formed both radical cations ($ M^{+•} $) and protonated molecules ([M + H]+) in photoionization. Better signal-to-noise ratios (S/N) were obtained in MS/MS analysis using the $ M^{+•} $ ions as precursor ions than using the [M + H]+ ions, and therefore the $ M^{+•} $ ions were selected for the precursor ions in selected reaction monitoring (SRM) analysis. Limits of detection (LODs) with the method ranged from 0.01 to 1 ng/mL, which correspond to instrumental LODs of 0.2–20 pg. Limits of quantitation ranged from 0.03 to 3 ng/mL. The mass spectrometric response to the analytes was linear (R ≥ 0.995) from the LOQ concentration level up to 100 ng/mL concentration, and intra-day repeatabilities were 5%–9%. In addition to the GC-μAPPI-MS study, the proof-of-principle of gas chromatography-microchip atmospheric pressure chemical ionization-Orbitrap MS (GC-μAPCI-Orbitrap MS) was demonstrated. Corona Discharge Select Reaction Monitoring MSTFA Select Reaction Monitoring Mode Sonic Spray Ionization Haapala, Markus aut Thevis, Mario aut Saarela, Ville aut Franssila, Sami aut Ketola, Raimo A. aut Kostiainen, Risto aut Kotiaho, Tapio aut Enthalten in Journal of the American Society for Mass Spectrometry Springer-Verlag, 1990 21(2010), 2 vom: Feb., Seite 310-316 (DE-627)130977357 (DE-600)1073671-2 (DE-576)277732093 1044-0305 nnns volume:21 year:2010 number:2 month:02 pages:310-316 https://doi.org/10.1016/j.jasms.2009.10.019 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-PHY SSG-OLC-CHE GBV_ILN_70 GBV_ILN_2004 GBV_ILN_4012 GBV_ILN_4125 GBV_ILN_4307 AR 21 2010 2 02 310-316 |
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10.1016/j.jasms.2009.10.019 doi (DE-627)OLC2097687016 (DE-He213)j.jasms.2009.10.019-p DE-627 ger DE-627 rakwb eng 530 VZ 11 ssgn Luosujärvi, Laura verfasserin aut Analysis of selective androgen receptor modulators by gas chromatography-microchip atmospheric pressure photoionization-mass spectrometry 2010 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © American Society for Mass Spectrometry 2010 Abstract A gas chromatography-microchip atmospheric pressure photoionization-mass spectrometric (GC-μAPPI-MS) method was developed and used for the analysis of three 2-quinolinone-derived selective androgen receptor modulators (SARMs). SARMs were analyzed from spiked urine samples, which were hydrolyzed and derivatized with N-methyl-N-(trimethylsilyl)trifluoroacetamide before analysis. Trimethylsilyl derivatives of SARMs formed both radical cations ($ M^{+•} $) and protonated molecules ([M + H]+) in photoionization. Better signal-to-noise ratios (S/N) were obtained in MS/MS analysis using the $ M^{+•} $ ions as precursor ions than using the [M + H]+ ions, and therefore the $ M^{+•} $ ions were selected for the precursor ions in selected reaction monitoring (SRM) analysis. Limits of detection (LODs) with the method ranged from 0.01 to 1 ng/mL, which correspond to instrumental LODs of 0.2–20 pg. Limits of quantitation ranged from 0.03 to 3 ng/mL. The mass spectrometric response to the analytes was linear (R ≥ 0.995) from the LOQ concentration level up to 100 ng/mL concentration, and intra-day repeatabilities were 5%–9%. In addition to the GC-μAPPI-MS study, the proof-of-principle of gas chromatography-microchip atmospheric pressure chemical ionization-Orbitrap MS (GC-μAPCI-Orbitrap MS) was demonstrated. Corona Discharge Select Reaction Monitoring MSTFA Select Reaction Monitoring Mode Sonic Spray Ionization Haapala, Markus aut Thevis, Mario aut Saarela, Ville aut Franssila, Sami aut Ketola, Raimo A. aut Kostiainen, Risto aut Kotiaho, Tapio aut Enthalten in Journal of the American Society for Mass Spectrometry Springer-Verlag, 1990 21(2010), 2 vom: Feb., Seite 310-316 (DE-627)130977357 (DE-600)1073671-2 (DE-576)277732093 1044-0305 nnns volume:21 year:2010 number:2 month:02 pages:310-316 https://doi.org/10.1016/j.jasms.2009.10.019 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-PHY SSG-OLC-CHE GBV_ILN_70 GBV_ILN_2004 GBV_ILN_4012 GBV_ILN_4125 GBV_ILN_4307 AR 21 2010 2 02 310-316 |
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10.1016/j.jasms.2009.10.019 doi (DE-627)OLC2097687016 (DE-He213)j.jasms.2009.10.019-p DE-627 ger DE-627 rakwb eng 530 VZ 11 ssgn Luosujärvi, Laura verfasserin aut Analysis of selective androgen receptor modulators by gas chromatography-microchip atmospheric pressure photoionization-mass spectrometry 2010 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © American Society for Mass Spectrometry 2010 Abstract A gas chromatography-microchip atmospheric pressure photoionization-mass spectrometric (GC-μAPPI-MS) method was developed and used for the analysis of three 2-quinolinone-derived selective androgen receptor modulators (SARMs). SARMs were analyzed from spiked urine samples, which were hydrolyzed and derivatized with N-methyl-N-(trimethylsilyl)trifluoroacetamide before analysis. Trimethylsilyl derivatives of SARMs formed both radical cations ($ M^{+•} $) and protonated molecules ([M + H]+) in photoionization. Better signal-to-noise ratios (S/N) were obtained in MS/MS analysis using the $ M^{+•} $ ions as precursor ions than using the [M + H]+ ions, and therefore the $ M^{+•} $ ions were selected for the precursor ions in selected reaction monitoring (SRM) analysis. Limits of detection (LODs) with the method ranged from 0.01 to 1 ng/mL, which correspond to instrumental LODs of 0.2–20 pg. Limits of quantitation ranged from 0.03 to 3 ng/mL. The mass spectrometric response to the analytes was linear (R ≥ 0.995) from the LOQ concentration level up to 100 ng/mL concentration, and intra-day repeatabilities were 5%–9%. In addition to the GC-μAPPI-MS study, the proof-of-principle of gas chromatography-microchip atmospheric pressure chemical ionization-Orbitrap MS (GC-μAPCI-Orbitrap MS) was demonstrated. Corona Discharge Select Reaction Monitoring MSTFA Select Reaction Monitoring Mode Sonic Spray Ionization Haapala, Markus aut Thevis, Mario aut Saarela, Ville aut Franssila, Sami aut Ketola, Raimo A. aut Kostiainen, Risto aut Kotiaho, Tapio aut Enthalten in Journal of the American Society for Mass Spectrometry Springer-Verlag, 1990 21(2010), 2 vom: Feb., Seite 310-316 (DE-627)130977357 (DE-600)1073671-2 (DE-576)277732093 1044-0305 nnns volume:21 year:2010 number:2 month:02 pages:310-316 https://doi.org/10.1016/j.jasms.2009.10.019 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-PHY SSG-OLC-CHE GBV_ILN_70 GBV_ILN_2004 GBV_ILN_4012 GBV_ILN_4125 GBV_ILN_4307 AR 21 2010 2 02 310-316 |
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10.1016/j.jasms.2009.10.019 doi (DE-627)OLC2097687016 (DE-He213)j.jasms.2009.10.019-p DE-627 ger DE-627 rakwb eng 530 VZ 11 ssgn Luosujärvi, Laura verfasserin aut Analysis of selective androgen receptor modulators by gas chromatography-microchip atmospheric pressure photoionization-mass spectrometry 2010 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © American Society for Mass Spectrometry 2010 Abstract A gas chromatography-microchip atmospheric pressure photoionization-mass spectrometric (GC-μAPPI-MS) method was developed and used for the analysis of three 2-quinolinone-derived selective androgen receptor modulators (SARMs). SARMs were analyzed from spiked urine samples, which were hydrolyzed and derivatized with N-methyl-N-(trimethylsilyl)trifluoroacetamide before analysis. Trimethylsilyl derivatives of SARMs formed both radical cations ($ M^{+•} $) and protonated molecules ([M + H]+) in photoionization. Better signal-to-noise ratios (S/N) were obtained in MS/MS analysis using the $ M^{+•} $ ions as precursor ions than using the [M + H]+ ions, and therefore the $ M^{+•} $ ions were selected for the precursor ions in selected reaction monitoring (SRM) analysis. Limits of detection (LODs) with the method ranged from 0.01 to 1 ng/mL, which correspond to instrumental LODs of 0.2–20 pg. Limits of quantitation ranged from 0.03 to 3 ng/mL. The mass spectrometric response to the analytes was linear (R ≥ 0.995) from the LOQ concentration level up to 100 ng/mL concentration, and intra-day repeatabilities were 5%–9%. In addition to the GC-μAPPI-MS study, the proof-of-principle of gas chromatography-microchip atmospheric pressure chemical ionization-Orbitrap MS (GC-μAPCI-Orbitrap MS) was demonstrated. Corona Discharge Select Reaction Monitoring MSTFA Select Reaction Monitoring Mode Sonic Spray Ionization Haapala, Markus aut Thevis, Mario aut Saarela, Ville aut Franssila, Sami aut Ketola, Raimo A. aut Kostiainen, Risto aut Kotiaho, Tapio aut Enthalten in Journal of the American Society for Mass Spectrometry Springer-Verlag, 1990 21(2010), 2 vom: Feb., Seite 310-316 (DE-627)130977357 (DE-600)1073671-2 (DE-576)277732093 1044-0305 nnns volume:21 year:2010 number:2 month:02 pages:310-316 https://doi.org/10.1016/j.jasms.2009.10.019 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-PHY SSG-OLC-CHE GBV_ILN_70 GBV_ILN_2004 GBV_ILN_4012 GBV_ILN_4125 GBV_ILN_4307 AR 21 2010 2 02 310-316 |
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10.1016/j.jasms.2009.10.019 doi (DE-627)OLC2097687016 (DE-He213)j.jasms.2009.10.019-p DE-627 ger DE-627 rakwb eng 530 VZ 11 ssgn Luosujärvi, Laura verfasserin aut Analysis of selective androgen receptor modulators by gas chromatography-microchip atmospheric pressure photoionization-mass spectrometry 2010 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © American Society for Mass Spectrometry 2010 Abstract A gas chromatography-microchip atmospheric pressure photoionization-mass spectrometric (GC-μAPPI-MS) method was developed and used for the analysis of three 2-quinolinone-derived selective androgen receptor modulators (SARMs). SARMs were analyzed from spiked urine samples, which were hydrolyzed and derivatized with N-methyl-N-(trimethylsilyl)trifluoroacetamide before analysis. Trimethylsilyl derivatives of SARMs formed both radical cations ($ M^{+•} $) and protonated molecules ([M + H]+) in photoionization. Better signal-to-noise ratios (S/N) were obtained in MS/MS analysis using the $ M^{+•} $ ions as precursor ions than using the [M + H]+ ions, and therefore the $ M^{+•} $ ions were selected for the precursor ions in selected reaction monitoring (SRM) analysis. Limits of detection (LODs) with the method ranged from 0.01 to 1 ng/mL, which correspond to instrumental LODs of 0.2–20 pg. Limits of quantitation ranged from 0.03 to 3 ng/mL. The mass spectrometric response to the analytes was linear (R ≥ 0.995) from the LOQ concentration level up to 100 ng/mL concentration, and intra-day repeatabilities were 5%–9%. In addition to the GC-μAPPI-MS study, the proof-of-principle of gas chromatography-microchip atmospheric pressure chemical ionization-Orbitrap MS (GC-μAPCI-Orbitrap MS) was demonstrated. Corona Discharge Select Reaction Monitoring MSTFA Select Reaction Monitoring Mode Sonic Spray Ionization Haapala, Markus aut Thevis, Mario aut Saarela, Ville aut Franssila, Sami aut Ketola, Raimo A. aut Kostiainen, Risto aut Kotiaho, Tapio aut Enthalten in Journal of the American Society for Mass Spectrometry Springer-Verlag, 1990 21(2010), 2 vom: Feb., Seite 310-316 (DE-627)130977357 (DE-600)1073671-2 (DE-576)277732093 1044-0305 nnns volume:21 year:2010 number:2 month:02 pages:310-316 https://doi.org/10.1016/j.jasms.2009.10.019 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-PHY SSG-OLC-CHE GBV_ILN_70 GBV_ILN_2004 GBV_ILN_4012 GBV_ILN_4125 GBV_ILN_4307 AR 21 2010 2 02 310-316 |
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analysis of selective androgen receptor modulators by gas chromatography-microchip atmospheric pressure photoionization-mass spectrometry |
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Analysis of selective androgen receptor modulators by gas chromatography-microchip atmospheric pressure photoionization-mass spectrometry |
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Abstract A gas chromatography-microchip atmospheric pressure photoionization-mass spectrometric (GC-μAPPI-MS) method was developed and used for the analysis of three 2-quinolinone-derived selective androgen receptor modulators (SARMs). SARMs were analyzed from spiked urine samples, which were hydrolyzed and derivatized with N-methyl-N-(trimethylsilyl)trifluoroacetamide before analysis. Trimethylsilyl derivatives of SARMs formed both radical cations ($ M^{+•} $) and protonated molecules ([M + H]+) in photoionization. Better signal-to-noise ratios (S/N) were obtained in MS/MS analysis using the $ M^{+•} $ ions as precursor ions than using the [M + H]+ ions, and therefore the $ M^{+•} $ ions were selected for the precursor ions in selected reaction monitoring (SRM) analysis. Limits of detection (LODs) with the method ranged from 0.01 to 1 ng/mL, which correspond to instrumental LODs of 0.2–20 pg. Limits of quantitation ranged from 0.03 to 3 ng/mL. The mass spectrometric response to the analytes was linear (R ≥ 0.995) from the LOQ concentration level up to 100 ng/mL concentration, and intra-day repeatabilities were 5%–9%. In addition to the GC-μAPPI-MS study, the proof-of-principle of gas chromatography-microchip atmospheric pressure chemical ionization-Orbitrap MS (GC-μAPCI-Orbitrap MS) was demonstrated. © American Society for Mass Spectrometry 2010 |
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Abstract A gas chromatography-microchip atmospheric pressure photoionization-mass spectrometric (GC-μAPPI-MS) method was developed and used for the analysis of three 2-quinolinone-derived selective androgen receptor modulators (SARMs). SARMs were analyzed from spiked urine samples, which were hydrolyzed and derivatized with N-methyl-N-(trimethylsilyl)trifluoroacetamide before analysis. Trimethylsilyl derivatives of SARMs formed both radical cations ($ M^{+•} $) and protonated molecules ([M + H]+) in photoionization. Better signal-to-noise ratios (S/N) were obtained in MS/MS analysis using the $ M^{+•} $ ions as precursor ions than using the [M + H]+ ions, and therefore the $ M^{+•} $ ions were selected for the precursor ions in selected reaction monitoring (SRM) analysis. Limits of detection (LODs) with the method ranged from 0.01 to 1 ng/mL, which correspond to instrumental LODs of 0.2–20 pg. Limits of quantitation ranged from 0.03 to 3 ng/mL. The mass spectrometric response to the analytes was linear (R ≥ 0.995) from the LOQ concentration level up to 100 ng/mL concentration, and intra-day repeatabilities were 5%–9%. In addition to the GC-μAPPI-MS study, the proof-of-principle of gas chromatography-microchip atmospheric pressure chemical ionization-Orbitrap MS (GC-μAPCI-Orbitrap MS) was demonstrated. © American Society for Mass Spectrometry 2010 |
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