Enzymatic synthesis of glucuronides using lipophilic hollow fiber membranes

Abstract A lipophilic hollow fiber membrane preparation was used for the enzymatic glucuronidation of lipophilic aromatic compounds. A crude solubilized microsomal enzyme preparation was circulated on the external side of the lipophilic membrane while the phenol containing buffer solution was circulated through the internal side of the hollow fiber membrane. Phenols, which accumulate in and penetrate the lipophilic membrane, were converted by UDP-glucuronyltransferase to the corresponding glucuronides. During this process the lipophilic compounds are converted to hydrophilic substances, which are not able to rediffuse through the lipophilic membrane into the donor side of the hollow fiber module. The produced glucuronide is separated by means of a coupled dialysis with a module of hydrophilic surface (cellulose acetate), while the enzyme protein is retained. On the stripping side of the dialysing module the glucuronide can be separated by solid phase extraction (Lichroprep RP-18) while a continuous substitution of cofactor into this compartment is possible. UDPGA follows its own concentration gradient and migrates into the enzymatic mixture, where it is utilized. This new technique using hollow fiber modules offers completely new possibilities for long-term high-capacity, highly specific glucuronidation of phenolic compounds. Fields of application are not only the economical production of special glucuronides, but also the specific elimination of phenols from waste fluids or from serum and blood of patients. For the production of glucuronides by this technique the use of highly purified enzymes is not essential. Cheap crude enzyme preparations are quite adequate for an optimal reaction. Using a crude enzyme preparation with a specific batch activity of 13 nMol/min per mg of protein, the activity in the reactor system was observed to be 4.6 nMol/min of 2-naphtol glucuronide formed per mg of protein. This corresponds to 3.6 nMol/h of product formed per mg of protein per $ cm^{2} $ of hollow fiber surface. Using a membrane surface of 0.5 $ m^{2} $ the production of 18 mMol of glucuronide per h and mg protein can be achieved. Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Tegtmeier, F. [verfasserIn] Belsner, K. Brunner, G.

Format:	Artikel
Sprache:	Englisch

Erschienen:	1988

Schlagwörter:	Solid Phase Extraction Aromatic Compound Hollow Fiber Hollow Fiber Membrane Donor Side

Anmerkung:	© Springer-Verlag 1988

Übergeordnetes Werk:	Enthalten in: Bioprocess engineering - Springer-Verlag, 1986, 3(1988), 1 vom: März, Seite 43-47
Übergeordnetes Werk:	volume:3 ; year:1988 ; number:1 ; month:03 ; pages:43-47

Links:	Volltext

DOI / URN:	10.1007/BF00372859

Katalog-ID:	OLC2106594798

Internformat


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allfields	10.1007/BF00372859 doi (DE-627)OLC2106594798 (DE-He213)BF00372859-p DE-627 ger DE-627 rakwb eng 570 690 540 VZ 12 ssgn Tegtmeier, F. verfasserin aut Enzymatic synthesis of glucuronides using lipophilic hollow fiber membranes 1988 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag 1988 Abstract A lipophilic hollow fiber membrane preparation was used for the enzymatic glucuronidation of lipophilic aromatic compounds. A crude solubilized microsomal enzyme preparation was circulated on the external side of the lipophilic membrane while the phenol containing buffer solution was circulated through the internal side of the hollow fiber membrane. Phenols, which accumulate in and penetrate the lipophilic membrane, were converted by UDP-glucuronyltransferase to the corresponding glucuronides. During this process the lipophilic compounds are converted to hydrophilic substances, which are not able to rediffuse through the lipophilic membrane into the donor side of the hollow fiber module. The produced glucuronide is separated by means of a coupled dialysis with a module of hydrophilic surface (cellulose acetate), while the enzyme protein is retained. On the stripping side of the dialysing module the glucuronide can be separated by solid phase extraction (Lichroprep RP-18) while a continuous substitution of cofactor into this compartment is possible. UDPGA follows its own concentration gradient and migrates into the enzymatic mixture, where it is utilized. This new technique using hollow fiber modules offers completely new possibilities for long-term high-capacity, highly specific glucuronidation of phenolic compounds. Fields of application are not only the economical production of special glucuronides, but also the specific elimination of phenols from waste fluids or from serum and blood of patients. For the production of glucuronides by this technique the use of highly purified enzymes is not essential. Cheap crude enzyme preparations are quite adequate for an optimal reaction. Using a crude enzyme preparation with a specific batch activity of 13 nMol/min per mg of protein, the activity in the reactor system was observed to be 4.6 nMol/min of 2-naphtol glucuronide formed per mg of protein. This corresponds to 3.6 nMol/h of product formed per mg of protein per $ cm^{2} $ of hollow fiber surface. Using a membrane surface of 0.5 $ m^{2} $ the production of 18 mMol of glucuronide per h and mg protein can be achieved. Solid Phase Extraction Aromatic Compound Hollow Fiber Hollow Fiber Membrane Donor Side Belsner, K. aut Brunner, G. aut Enthalten in Bioprocess engineering Springer-Verlag, 1986 3(1988), 1 vom: März, Seite 43-47 (DE-627)129208922 (DE-600)55154-5 (DE-576)01445761X 0178-515X nnns volume:3 year:1988 number:1 month:03 pages:43-47 https://doi.org/10.1007/BF00372859 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-ARC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_11 GBV_ILN_23 GBV_ILN_40 GBV_ILN_65 GBV_ILN_70 GBV_ILN_2004 GBV_ILN_2006 GBV_ILN_2018 GBV_ILN_4046 GBV_ILN_4103 GBV_ILN_4307 GBV_ILN_4310 GBV_ILN_4323 AR 3 1988 1 03 43-47
spelling	10.1007/BF00372859 doi (DE-627)OLC2106594798 (DE-He213)BF00372859-p DE-627 ger DE-627 rakwb eng 570 690 540 VZ 12 ssgn Tegtmeier, F. verfasserin aut Enzymatic synthesis of glucuronides using lipophilic hollow fiber membranes 1988 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag 1988 Abstract A lipophilic hollow fiber membrane preparation was used for the enzymatic glucuronidation of lipophilic aromatic compounds. A crude solubilized microsomal enzyme preparation was circulated on the external side of the lipophilic membrane while the phenol containing buffer solution was circulated through the internal side of the hollow fiber membrane. Phenols, which accumulate in and penetrate the lipophilic membrane, were converted by UDP-glucuronyltransferase to the corresponding glucuronides. During this process the lipophilic compounds are converted to hydrophilic substances, which are not able to rediffuse through the lipophilic membrane into the donor side of the hollow fiber module. The produced glucuronide is separated by means of a coupled dialysis with a module of hydrophilic surface (cellulose acetate), while the enzyme protein is retained. On the stripping side of the dialysing module the glucuronide can be separated by solid phase extraction (Lichroprep RP-18) while a continuous substitution of cofactor into this compartment is possible. UDPGA follows its own concentration gradient and migrates into the enzymatic mixture, where it is utilized. This new technique using hollow fiber modules offers completely new possibilities for long-term high-capacity, highly specific glucuronidation of phenolic compounds. Fields of application are not only the economical production of special glucuronides, but also the specific elimination of phenols from waste fluids or from serum and blood of patients. For the production of glucuronides by this technique the use of highly purified enzymes is not essential. Cheap crude enzyme preparations are quite adequate for an optimal reaction. Using a crude enzyme preparation with a specific batch activity of 13 nMol/min per mg of protein, the activity in the reactor system was observed to be 4.6 nMol/min of 2-naphtol glucuronide formed per mg of protein. This corresponds to 3.6 nMol/h of product formed per mg of protein per $ cm^{2} $ of hollow fiber surface. Using a membrane surface of 0.5 $ m^{2} $ the production of 18 mMol of glucuronide per h and mg protein can be achieved. Solid Phase Extraction Aromatic Compound Hollow Fiber Hollow Fiber Membrane Donor Side Belsner, K. aut Brunner, G. aut Enthalten in Bioprocess engineering Springer-Verlag, 1986 3(1988), 1 vom: März, Seite 43-47 (DE-627)129208922 (DE-600)55154-5 (DE-576)01445761X 0178-515X nnns volume:3 year:1988 number:1 month:03 pages:43-47 https://doi.org/10.1007/BF00372859 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-ARC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_11 GBV_ILN_23 GBV_ILN_40 GBV_ILN_65 GBV_ILN_70 GBV_ILN_2004 GBV_ILN_2006 GBV_ILN_2018 GBV_ILN_4046 GBV_ILN_4103 GBV_ILN_4307 GBV_ILN_4310 GBV_ILN_4323 AR 3 1988 1 03 43-47
allfields_unstemmed	10.1007/BF00372859 doi (DE-627)OLC2106594798 (DE-He213)BF00372859-p DE-627 ger DE-627 rakwb eng 570 690 540 VZ 12 ssgn Tegtmeier, F. verfasserin aut Enzymatic synthesis of glucuronides using lipophilic hollow fiber membranes 1988 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag 1988 Abstract A lipophilic hollow fiber membrane preparation was used for the enzymatic glucuronidation of lipophilic aromatic compounds. A crude solubilized microsomal enzyme preparation was circulated on the external side of the lipophilic membrane while the phenol containing buffer solution was circulated through the internal side of the hollow fiber membrane. Phenols, which accumulate in and penetrate the lipophilic membrane, were converted by UDP-glucuronyltransferase to the corresponding glucuronides. During this process the lipophilic compounds are converted to hydrophilic substances, which are not able to rediffuse through the lipophilic membrane into the donor side of the hollow fiber module. The produced glucuronide is separated by means of a coupled dialysis with a module of hydrophilic surface (cellulose acetate), while the enzyme protein is retained. On the stripping side of the dialysing module the glucuronide can be separated by solid phase extraction (Lichroprep RP-18) while a continuous substitution of cofactor into this compartment is possible. UDPGA follows its own concentration gradient and migrates into the enzymatic mixture, where it is utilized. This new technique using hollow fiber modules offers completely new possibilities for long-term high-capacity, highly specific glucuronidation of phenolic compounds. Fields of application are not only the economical production of special glucuronides, but also the specific elimination of phenols from waste fluids or from serum and blood of patients. For the production of glucuronides by this technique the use of highly purified enzymes is not essential. Cheap crude enzyme preparations are quite adequate for an optimal reaction. Using a crude enzyme preparation with a specific batch activity of 13 nMol/min per mg of protein, the activity in the reactor system was observed to be 4.6 nMol/min of 2-naphtol glucuronide formed per mg of protein. This corresponds to 3.6 nMol/h of product formed per mg of protein per $ cm^{2} $ of hollow fiber surface. Using a membrane surface of 0.5 $ m^{2} $ the production of 18 mMol of glucuronide per h and mg protein can be achieved. Solid Phase Extraction Aromatic Compound Hollow Fiber Hollow Fiber Membrane Donor Side Belsner, K. aut Brunner, G. aut Enthalten in Bioprocess engineering Springer-Verlag, 1986 3(1988), 1 vom: März, Seite 43-47 (DE-627)129208922 (DE-600)55154-5 (DE-576)01445761X 0178-515X nnns volume:3 year:1988 number:1 month:03 pages:43-47 https://doi.org/10.1007/BF00372859 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-ARC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_11 GBV_ILN_23 GBV_ILN_40 GBV_ILN_65 GBV_ILN_70 GBV_ILN_2004 GBV_ILN_2006 GBV_ILN_2018 GBV_ILN_4046 GBV_ILN_4103 GBV_ILN_4307 GBV_ILN_4310 GBV_ILN_4323 AR 3 1988 1 03 43-47
allfieldsGer	10.1007/BF00372859 doi (DE-627)OLC2106594798 (DE-He213)BF00372859-p DE-627 ger DE-627 rakwb eng 570 690 540 VZ 12 ssgn Tegtmeier, F. verfasserin aut Enzymatic synthesis of glucuronides using lipophilic hollow fiber membranes 1988 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag 1988 Abstract A lipophilic hollow fiber membrane preparation was used for the enzymatic glucuronidation of lipophilic aromatic compounds. A crude solubilized microsomal enzyme preparation was circulated on the external side of the lipophilic membrane while the phenol containing buffer solution was circulated through the internal side of the hollow fiber membrane. Phenols, which accumulate in and penetrate the lipophilic membrane, were converted by UDP-glucuronyltransferase to the corresponding glucuronides. During this process the lipophilic compounds are converted to hydrophilic substances, which are not able to rediffuse through the lipophilic membrane into the donor side of the hollow fiber module. The produced glucuronide is separated by means of a coupled dialysis with a module of hydrophilic surface (cellulose acetate), while the enzyme protein is retained. On the stripping side of the dialysing module the glucuronide can be separated by solid phase extraction (Lichroprep RP-18) while a continuous substitution of cofactor into this compartment is possible. UDPGA follows its own concentration gradient and migrates into the enzymatic mixture, where it is utilized. This new technique using hollow fiber modules offers completely new possibilities for long-term high-capacity, highly specific glucuronidation of phenolic compounds. Fields of application are not only the economical production of special glucuronides, but also the specific elimination of phenols from waste fluids or from serum and blood of patients. For the production of glucuronides by this technique the use of highly purified enzymes is not essential. Cheap crude enzyme preparations are quite adequate for an optimal reaction. Using a crude enzyme preparation with a specific batch activity of 13 nMol/min per mg of protein, the activity in the reactor system was observed to be 4.6 nMol/min of 2-naphtol glucuronide formed per mg of protein. This corresponds to 3.6 nMol/h of product formed per mg of protein per $ cm^{2} $ of hollow fiber surface. Using a membrane surface of 0.5 $ m^{2} $ the production of 18 mMol of glucuronide per h and mg protein can be achieved. Solid Phase Extraction Aromatic Compound Hollow Fiber Hollow Fiber Membrane Donor Side Belsner, K. aut Brunner, G. aut Enthalten in Bioprocess engineering Springer-Verlag, 1986 3(1988), 1 vom: März, Seite 43-47 (DE-627)129208922 (DE-600)55154-5 (DE-576)01445761X 0178-515X nnns volume:3 year:1988 number:1 month:03 pages:43-47 https://doi.org/10.1007/BF00372859 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-ARC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_11 GBV_ILN_23 GBV_ILN_40 GBV_ILN_65 GBV_ILN_70 GBV_ILN_2004 GBV_ILN_2006 GBV_ILN_2018 GBV_ILN_4046 GBV_ILN_4103 GBV_ILN_4307 GBV_ILN_4310 GBV_ILN_4323 AR 3 1988 1 03 43-47
allfieldsSound	10.1007/BF00372859 doi (DE-627)OLC2106594798 (DE-He213)BF00372859-p DE-627 ger DE-627 rakwb eng 570 690 540 VZ 12 ssgn Tegtmeier, F. verfasserin aut Enzymatic synthesis of glucuronides using lipophilic hollow fiber membranes 1988 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag 1988 Abstract A lipophilic hollow fiber membrane preparation was used for the enzymatic glucuronidation of lipophilic aromatic compounds. A crude solubilized microsomal enzyme preparation was circulated on the external side of the lipophilic membrane while the phenol containing buffer solution was circulated through the internal side of the hollow fiber membrane. Phenols, which accumulate in and penetrate the lipophilic membrane, were converted by UDP-glucuronyltransferase to the corresponding glucuronides. During this process the lipophilic compounds are converted to hydrophilic substances, which are not able to rediffuse through the lipophilic membrane into the donor side of the hollow fiber module. The produced glucuronide is separated by means of a coupled dialysis with a module of hydrophilic surface (cellulose acetate), while the enzyme protein is retained. On the stripping side of the dialysing module the glucuronide can be separated by solid phase extraction (Lichroprep RP-18) while a continuous substitution of cofactor into this compartment is possible. UDPGA follows its own concentration gradient and migrates into the enzymatic mixture, where it is utilized. This new technique using hollow fiber modules offers completely new possibilities for long-term high-capacity, highly specific glucuronidation of phenolic compounds. Fields of application are not only the economical production of special glucuronides, but also the specific elimination of phenols from waste fluids or from serum and blood of patients. For the production of glucuronides by this technique the use of highly purified enzymes is not essential. Cheap crude enzyme preparations are quite adequate for an optimal reaction. Using a crude enzyme preparation with a specific batch activity of 13 nMol/min per mg of protein, the activity in the reactor system was observed to be 4.6 nMol/min of 2-naphtol glucuronide formed per mg of protein. This corresponds to 3.6 nMol/h of product formed per mg of protein per $ cm^{2} $ of hollow fiber surface. Using a membrane surface of 0.5 $ m^{2} $ the production of 18 mMol of glucuronide per h and mg protein can be achieved. Solid Phase Extraction Aromatic Compound Hollow Fiber Hollow Fiber Membrane Donor Side Belsner, K. aut Brunner, G. aut Enthalten in Bioprocess engineering Springer-Verlag, 1986 3(1988), 1 vom: März, Seite 43-47 (DE-627)129208922 (DE-600)55154-5 (DE-576)01445761X 0178-515X nnns volume:3 year:1988 number:1 month:03 pages:43-47 https://doi.org/10.1007/BF00372859 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-ARC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_11 GBV_ILN_23 GBV_ILN_40 GBV_ILN_65 GBV_ILN_70 GBV_ILN_2004 GBV_ILN_2006 GBV_ILN_2018 GBV_ILN_4046 GBV_ILN_4103 GBV_ILN_4307 GBV_ILN_4310 GBV_ILN_4323 AR 3 1988 1 03 43-47
language	English
source	Enthalten in Bioprocess engineering 3(1988), 1 vom: März, Seite 43-47 volume:3 year:1988 number:1 month:03 pages:43-47
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A crude solubilized microsomal enzyme preparation was circulated on the external side of the lipophilic membrane while the phenol containing buffer solution was circulated through the internal side of the hollow fiber membrane. Phenols, which accumulate in and penetrate the lipophilic membrane, were converted by UDP-glucuronyltransferase to the corresponding glucuronides. During this process the lipophilic compounds are converted to hydrophilic substances, which are not able to rediffuse through the lipophilic membrane into the donor side of the hollow fiber module. The produced glucuronide is separated by means of a coupled dialysis with a module of hydrophilic surface (cellulose acetate), while the enzyme protein is retained. On the stripping side of the dialysing module the glucuronide can be separated by solid phase extraction (Lichroprep RP-18) while a continuous substitution of cofactor into this compartment is possible. UDPGA follows its own concentration gradient and migrates into the enzymatic mixture, where it is utilized. This new technique using hollow fiber modules offers completely new possibilities for long-term high-capacity, highly specific glucuronidation of phenolic compounds. Fields of application are not only the economical production of special glucuronides, but also the specific elimination of phenols from waste fluids or from serum and blood of patients. For the production of glucuronides by this technique the use of highly purified enzymes is not essential. Cheap crude enzyme preparations are quite adequate for an optimal reaction. Using a crude enzyme preparation with a specific batch activity of 13 nMol/min per mg of protein, the activity in the reactor system was observed to be 4.6 nMol/min of 2-naphtol glucuronide formed per mg of protein. This corresponds to 3.6 nMol/h of product formed per mg of protein per $ cm^{2} $ of hollow fiber surface. 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author	Tegtmeier, F.
spellingShingle	Tegtmeier, F. ddc 570 ssgn 12 misc Solid Phase Extraction misc Aromatic Compound misc Hollow Fiber misc Hollow Fiber Membrane misc Donor Side Enzymatic synthesis of glucuronides using lipophilic hollow fiber membranes
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topic_title	570 690 540 VZ 12 ssgn Enzymatic synthesis of glucuronides using lipophilic hollow fiber membranes Solid Phase Extraction Aromatic Compound Hollow Fiber Hollow Fiber Membrane Donor Side
topic	ddc 570 ssgn 12 misc Solid Phase Extraction misc Aromatic Compound misc Hollow Fiber misc Hollow Fiber Membrane misc Donor Side
topic_unstemmed	ddc 570 ssgn 12 misc Solid Phase Extraction misc Aromatic Compound misc Hollow Fiber misc Hollow Fiber Membrane misc Donor Side
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title	Enzymatic synthesis of glucuronides using lipophilic hollow fiber membranes
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title_full	Enzymatic synthesis of glucuronides using lipophilic hollow fiber membranes
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title_sort	enzymatic synthesis of glucuronides using lipophilic hollow fiber membranes
title_auth	Enzymatic synthesis of glucuronides using lipophilic hollow fiber membranes
abstract	Abstract A lipophilic hollow fiber membrane preparation was used for the enzymatic glucuronidation of lipophilic aromatic compounds. A crude solubilized microsomal enzyme preparation was circulated on the external side of the lipophilic membrane while the phenol containing buffer solution was circulated through the internal side of the hollow fiber membrane. Phenols, which accumulate in and penetrate the lipophilic membrane, were converted by UDP-glucuronyltransferase to the corresponding glucuronides. During this process the lipophilic compounds are converted to hydrophilic substances, which are not able to rediffuse through the lipophilic membrane into the donor side of the hollow fiber module. The produced glucuronide is separated by means of a coupled dialysis with a module of hydrophilic surface (cellulose acetate), while the enzyme protein is retained. On the stripping side of the dialysing module the glucuronide can be separated by solid phase extraction (Lichroprep RP-18) while a continuous substitution of cofactor into this compartment is possible. UDPGA follows its own concentration gradient and migrates into the enzymatic mixture, where it is utilized. This new technique using hollow fiber modules offers completely new possibilities for long-term high-capacity, highly specific glucuronidation of phenolic compounds. Fields of application are not only the economical production of special glucuronides, but also the specific elimination of phenols from waste fluids or from serum and blood of patients. For the production of glucuronides by this technique the use of highly purified enzymes is not essential. Cheap crude enzyme preparations are quite adequate for an optimal reaction. Using a crude enzyme preparation with a specific batch activity of 13 nMol/min per mg of protein, the activity in the reactor system was observed to be 4.6 nMol/min of 2-naphtol glucuronide formed per mg of protein. This corresponds to 3.6 nMol/h of product formed per mg of protein per $ cm^{2} $ of hollow fiber surface. Using a membrane surface of 0.5 $ m^{2} $ the production of 18 mMol of glucuronide per h and mg protein can be achieved. © Springer-Verlag 1988
abstractGer	Abstract A lipophilic hollow fiber membrane preparation was used for the enzymatic glucuronidation of lipophilic aromatic compounds. A crude solubilized microsomal enzyme preparation was circulated on the external side of the lipophilic membrane while the phenol containing buffer solution was circulated through the internal side of the hollow fiber membrane. Phenols, which accumulate in and penetrate the lipophilic membrane, were converted by UDP-glucuronyltransferase to the corresponding glucuronides. During this process the lipophilic compounds are converted to hydrophilic substances, which are not able to rediffuse through the lipophilic membrane into the donor side of the hollow fiber module. The produced glucuronide is separated by means of a coupled dialysis with a module of hydrophilic surface (cellulose acetate), while the enzyme protein is retained. On the stripping side of the dialysing module the glucuronide can be separated by solid phase extraction (Lichroprep RP-18) while a continuous substitution of cofactor into this compartment is possible. UDPGA follows its own concentration gradient and migrates into the enzymatic mixture, where it is utilized. This new technique using hollow fiber modules offers completely new possibilities for long-term high-capacity, highly specific glucuronidation of phenolic compounds. Fields of application are not only the economical production of special glucuronides, but also the specific elimination of phenols from waste fluids or from serum and blood of patients. For the production of glucuronides by this technique the use of highly purified enzymes is not essential. Cheap crude enzyme preparations are quite adequate for an optimal reaction. Using a crude enzyme preparation with a specific batch activity of 13 nMol/min per mg of protein, the activity in the reactor system was observed to be 4.6 nMol/min of 2-naphtol glucuronide formed per mg of protein. This corresponds to 3.6 nMol/h of product formed per mg of protein per $ cm^{2} $ of hollow fiber surface. Using a membrane surface of 0.5 $ m^{2} $ the production of 18 mMol of glucuronide per h and mg protein can be achieved. © Springer-Verlag 1988
abstract_unstemmed	Abstract A lipophilic hollow fiber membrane preparation was used for the enzymatic glucuronidation of lipophilic aromatic compounds. A crude solubilized microsomal enzyme preparation was circulated on the external side of the lipophilic membrane while the phenol containing buffer solution was circulated through the internal side of the hollow fiber membrane. Phenols, which accumulate in and penetrate the lipophilic membrane, were converted by UDP-glucuronyltransferase to the corresponding glucuronides. During this process the lipophilic compounds are converted to hydrophilic substances, which are not able to rediffuse through the lipophilic membrane into the donor side of the hollow fiber module. The produced glucuronide is separated by means of a coupled dialysis with a module of hydrophilic surface (cellulose acetate), while the enzyme protein is retained. On the stripping side of the dialysing module the glucuronide can be separated by solid phase extraction (Lichroprep RP-18) while a continuous substitution of cofactor into this compartment is possible. UDPGA follows its own concentration gradient and migrates into the enzymatic mixture, where it is utilized. This new technique using hollow fiber modules offers completely new possibilities for long-term high-capacity, highly specific glucuronidation of phenolic compounds. Fields of application are not only the economical production of special glucuronides, but also the specific elimination of phenols from waste fluids or from serum and blood of patients. For the production of glucuronides by this technique the use of highly purified enzymes is not essential. Cheap crude enzyme preparations are quite adequate for an optimal reaction. Using a crude enzyme preparation with a specific batch activity of 13 nMol/min per mg of protein, the activity in the reactor system was observed to be 4.6 nMol/min of 2-naphtol glucuronide formed per mg of protein. This corresponds to 3.6 nMol/h of product formed per mg of protein per $ cm^{2} $ of hollow fiber surface. Using a membrane surface of 0.5 $ m^{2} $ the production of 18 mMol of glucuronide per h and mg protein can be achieved. © Springer-Verlag 1988
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title_short	Enzymatic synthesis of glucuronides using lipophilic hollow fiber membranes
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