Enhanced amylase production by Bacillus subtilis using a dual exponential feeding strategy
Abstract A recombinant Bacillus subtilis strain (ATCC 31784) haboring the plasmid pC194 with a thermostable α-amylase gene was cultured in a 22-l B. Braun Biostat C fermenter. Traditional batch operations suffer from low cell mass and protein productions because a high initial glucose concentration...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Huang, Hanjing [verfasserIn] |
|---|
| Format: |
Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2004 |
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| Schlagwörter: |
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| Anmerkung: |
© Springer-Verlag 2004 |
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| Übergeordnetes Werk: |
Enthalten in: Bioprocess and biosystems engineering - Springer-Verlag, 2001, 27(2004), 1 vom: 18. Nov., Seite 63-69 |
|---|---|
| Übergeordnetes Werk: |
volume:27 ; year:2004 ; number:1 ; day:18 ; month:11 ; pages:63-69 |
| Links: |
|---|
| DOI / URN: |
10.1007/s00449-004-0391-z |
|---|
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OLC2106609590 |
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| 520 | |a Abstract A recombinant Bacillus subtilis strain (ATCC 31784) haboring the plasmid pC194 with a thermostable α-amylase gene was cultured in a 22-l B. Braun Biostat C fermenter. Traditional batch operations suffer from low cell mass and protein productions because a high initial glucose concentration causes substrate inhibition and also product inhibition due to acetate accumulation. An exponential fed-batch strategy to prevent these inhibitions was developed in this work. The host strain is auxotrophic for phenylalanine, tyrosine and tryptophan. Due to low solubilities of tyrosine and tryptophan in the feed stream, tyrosine and tryptophan were dissolved separately in ammonia water to form a second feed stream. By dual feeding both streams at different exponential feed rates, a high cell density of 17.6 g/l and a final α-amylase activity of 41.4 U/ml and the overall biomass yield of 0.39 g cell/g glucose were achieved. | ||
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10.1007/s00449-004-0391-z doi (DE-627)OLC2106609590 (DE-He213)s00449-004-0391-z-p DE-627 ger DE-627 rakwb eng 660 VZ 570 690 540 VZ 12 ssgn Huang, Hanjing verfasserin aut Enhanced amylase production by Bacillus subtilis using a dual exponential feeding strategy 2004 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag 2004 Abstract A recombinant Bacillus subtilis strain (ATCC 31784) haboring the plasmid pC194 with a thermostable α-amylase gene was cultured in a 22-l B. Braun Biostat C fermenter. Traditional batch operations suffer from low cell mass and protein productions because a high initial glucose concentration causes substrate inhibition and also product inhibition due to acetate accumulation. An exponential fed-batch strategy to prevent these inhibitions was developed in this work. The host strain is auxotrophic for phenylalanine, tyrosine and tryptophan. Due to low solubilities of tyrosine and tryptophan in the feed stream, tyrosine and tryptophan were dissolved separately in ammonia water to form a second feed stream. By dual feeding both streams at different exponential feed rates, a high cell density of 17.6 g/l and a final α-amylase activity of 41.4 U/ml and the overall biomass yield of 0.39 g cell/g glucose were achieved. Fermentation Dual feeding Exponential fed-batch culture Ridgway, Darin aut Gu, Tingyue aut Moo-Young, Murray aut Enthalten in Bioprocess and biosystems engineering Springer-Verlag, 2001 27(2004), 1 vom: 18. Nov., Seite 63-69 (DE-627)333469763 (DE-600)2056063-1 (DE-576)094533709 1615-7591 nnns volume:27 year:2004 number:1 day:18 month:11 pages:63-69 https://doi.org/10.1007/s00449-004-0391-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-ARC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_11 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_70 GBV_ILN_2006 GBV_ILN_2014 GBV_ILN_2018 GBV_ILN_4046 GBV_ILN_4277 GBV_ILN_4307 AR 27 2004 1 18 11 63-69 |
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10.1007/s00449-004-0391-z doi (DE-627)OLC2106609590 (DE-He213)s00449-004-0391-z-p DE-627 ger DE-627 rakwb eng 660 VZ 570 690 540 VZ 12 ssgn Huang, Hanjing verfasserin aut Enhanced amylase production by Bacillus subtilis using a dual exponential feeding strategy 2004 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag 2004 Abstract A recombinant Bacillus subtilis strain (ATCC 31784) haboring the plasmid pC194 with a thermostable α-amylase gene was cultured in a 22-l B. Braun Biostat C fermenter. Traditional batch operations suffer from low cell mass and protein productions because a high initial glucose concentration causes substrate inhibition and also product inhibition due to acetate accumulation. An exponential fed-batch strategy to prevent these inhibitions was developed in this work. The host strain is auxotrophic for phenylalanine, tyrosine and tryptophan. Due to low solubilities of tyrosine and tryptophan in the feed stream, tyrosine and tryptophan were dissolved separately in ammonia water to form a second feed stream. By dual feeding both streams at different exponential feed rates, a high cell density of 17.6 g/l and a final α-amylase activity of 41.4 U/ml and the overall biomass yield of 0.39 g cell/g glucose were achieved. Fermentation Dual feeding Exponential fed-batch culture Ridgway, Darin aut Gu, Tingyue aut Moo-Young, Murray aut Enthalten in Bioprocess and biosystems engineering Springer-Verlag, 2001 27(2004), 1 vom: 18. Nov., Seite 63-69 (DE-627)333469763 (DE-600)2056063-1 (DE-576)094533709 1615-7591 nnns volume:27 year:2004 number:1 day:18 month:11 pages:63-69 https://doi.org/10.1007/s00449-004-0391-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-ARC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_11 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_70 GBV_ILN_2006 GBV_ILN_2014 GBV_ILN_2018 GBV_ILN_4046 GBV_ILN_4277 GBV_ILN_4307 AR 27 2004 1 18 11 63-69 |
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Enhanced amylase production by Bacillus subtilis using a dual exponential feeding strategy |
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Enhanced amylase production by Bacillus subtilis using a dual exponential feeding strategy |
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Huang, Hanjing |
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Bioprocess and biosystems engineering |
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Huang, Hanjing Ridgway, Darin Gu, Tingyue Moo-Young, Murray |
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enhanced amylase production by bacillus subtilis using a dual exponential feeding strategy |
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Enhanced amylase production by Bacillus subtilis using a dual exponential feeding strategy |
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Abstract A recombinant Bacillus subtilis strain (ATCC 31784) haboring the plasmid pC194 with a thermostable α-amylase gene was cultured in a 22-l B. Braun Biostat C fermenter. Traditional batch operations suffer from low cell mass and protein productions because a high initial glucose concentration causes substrate inhibition and also product inhibition due to acetate accumulation. An exponential fed-batch strategy to prevent these inhibitions was developed in this work. The host strain is auxotrophic for phenylalanine, tyrosine and tryptophan. Due to low solubilities of tyrosine and tryptophan in the feed stream, tyrosine and tryptophan were dissolved separately in ammonia water to form a second feed stream. By dual feeding both streams at different exponential feed rates, a high cell density of 17.6 g/l and a final α-amylase activity of 41.4 U/ml and the overall biomass yield of 0.39 g cell/g glucose were achieved. © Springer-Verlag 2004 |
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Abstract A recombinant Bacillus subtilis strain (ATCC 31784) haboring the plasmid pC194 with a thermostable α-amylase gene was cultured in a 22-l B. Braun Biostat C fermenter. Traditional batch operations suffer from low cell mass and protein productions because a high initial glucose concentration causes substrate inhibition and also product inhibition due to acetate accumulation. An exponential fed-batch strategy to prevent these inhibitions was developed in this work. The host strain is auxotrophic for phenylalanine, tyrosine and tryptophan. Due to low solubilities of tyrosine and tryptophan in the feed stream, tyrosine and tryptophan were dissolved separately in ammonia water to form a second feed stream. By dual feeding both streams at different exponential feed rates, a high cell density of 17.6 g/l and a final α-amylase activity of 41.4 U/ml and the overall biomass yield of 0.39 g cell/g glucose were achieved. © Springer-Verlag 2004 |
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Abstract A recombinant Bacillus subtilis strain (ATCC 31784) haboring the plasmid pC194 with a thermostable α-amylase gene was cultured in a 22-l B. Braun Biostat C fermenter. Traditional batch operations suffer from low cell mass and protein productions because a high initial glucose concentration causes substrate inhibition and also product inhibition due to acetate accumulation. An exponential fed-batch strategy to prevent these inhibitions was developed in this work. The host strain is auxotrophic for phenylalanine, tyrosine and tryptophan. Due to low solubilities of tyrosine and tryptophan in the feed stream, tyrosine and tryptophan were dissolved separately in ammonia water to form a second feed stream. By dual feeding both streams at different exponential feed rates, a high cell density of 17.6 g/l and a final α-amylase activity of 41.4 U/ml and the overall biomass yield of 0.39 g cell/g glucose were achieved. © Springer-Verlag 2004 |
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