Enhanced amylase production by Bacillus subtilis using a dual exponential feeding strategy
Abstract A recombinant Bacillus subtilis strain (ATCC 31784) haboring the plasmid pC194 with a thermostable α-amylase gene was cultured in a 22-l B. Braun Biostat C fermenter. Traditional batch operations suffer from low cell mass and protein productions because a high initial glucose concentration...
Ausführliche Beschreibung
Autor*in: |
Huang, Hanjing [verfasserIn] |
---|
Format: |
Artikel |
---|---|
Sprache: |
Englisch |
Erschienen: |
2004 |
---|
Schlagwörter: |
---|
Anmerkung: |
© Springer-Verlag 2004 |
---|
Übergeordnetes Werk: |
Enthalten in: Bioprocess and biosystems engineering - Springer-Verlag, 2001, 27(2004), 1 vom: 18. Nov., Seite 63-69 |
---|---|
Übergeordnetes Werk: |
volume:27 ; year:2004 ; number:1 ; day:18 ; month:11 ; pages:63-69 |
Links: |
---|
DOI / URN: |
10.1007/s00449-004-0391-z |
---|
Katalog-ID: |
OLC2106609590 |
---|
LEADER | 01000naa a22002652 4500 | ||
---|---|---|---|
001 | OLC2106609590 | ||
003 | DE-627 | ||
005 | 20230403073226.0 | ||
007 | tu | ||
008 | 230403s2004 xx ||||| 00| ||eng c | ||
024 | 7 | |a 10.1007/s00449-004-0391-z |2 doi | |
035 | |a (DE-627)OLC2106609590 | ||
035 | |a (DE-He213)s00449-004-0391-z-p | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
082 | 0 | 4 | |a 660 |q VZ |
082 | 0 | 4 | |a 570 |a 690 |a 540 |q VZ |
084 | |a 12 |2 ssgn | ||
100 | 1 | |a Huang, Hanjing |e verfasserin |4 aut | |
245 | 1 | 0 | |a Enhanced amylase production by Bacillus subtilis using a dual exponential feeding strategy |
264 | 1 | |c 2004 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
338 | |a Band |b nc |2 rdacarrier | ||
500 | |a © Springer-Verlag 2004 | ||
520 | |a Abstract A recombinant Bacillus subtilis strain (ATCC 31784) haboring the plasmid pC194 with a thermostable α-amylase gene was cultured in a 22-l B. Braun Biostat C fermenter. Traditional batch operations suffer from low cell mass and protein productions because a high initial glucose concentration causes substrate inhibition and also product inhibition due to acetate accumulation. An exponential fed-batch strategy to prevent these inhibitions was developed in this work. The host strain is auxotrophic for phenylalanine, tyrosine and tryptophan. Due to low solubilities of tyrosine and tryptophan in the feed stream, tyrosine and tryptophan were dissolved separately in ammonia water to form a second feed stream. By dual feeding both streams at different exponential feed rates, a high cell density of 17.6 g/l and a final α-amylase activity of 41.4 U/ml and the overall biomass yield of 0.39 g cell/g glucose were achieved. | ||
650 | 4 | |a Fermentation | |
650 | 4 | |a Dual feeding | |
650 | 4 | |a Exponential fed-batch culture | |
700 | 1 | |a Ridgway, Darin |4 aut | |
700 | 1 | |a Gu, Tingyue |4 aut | |
700 | 1 | |a Moo-Young, Murray |4 aut | |
773 | 0 | 8 | |i Enthalten in |t Bioprocess and biosystems engineering |d Springer-Verlag, 2001 |g 27(2004), 1 vom: 18. Nov., Seite 63-69 |w (DE-627)333469763 |w (DE-600)2056063-1 |w (DE-576)094533709 |x 1615-7591 |7 nnns |
773 | 1 | 8 | |g volume:27 |g year:2004 |g number:1 |g day:18 |g month:11 |g pages:63-69 |
856 | 4 | 1 | |u https://doi.org/10.1007/s00449-004-0391-z |z lizenzpflichtig |3 Volltext |
912 | |a GBV_USEFLAG_A | ||
912 | |a SYSFLAG_A | ||
912 | |a GBV_OLC | ||
912 | |a SSG-OLC-UMW | ||
912 | |a SSG-OLC-ARC | ||
912 | |a SSG-OLC-TEC | ||
912 | |a SSG-OLC-CHE | ||
912 | |a GBV_ILN_11 | ||
912 | |a GBV_ILN_23 | ||
912 | |a GBV_ILN_24 | ||
912 | |a GBV_ILN_31 | ||
912 | |a GBV_ILN_32 | ||
912 | |a GBV_ILN_70 | ||
912 | |a GBV_ILN_2006 | ||
912 | |a GBV_ILN_2014 | ||
912 | |a GBV_ILN_2018 | ||
912 | |a GBV_ILN_4046 | ||
912 | |a GBV_ILN_4277 | ||
912 | |a GBV_ILN_4307 | ||
951 | |a AR | ||
952 | |d 27 |j 2004 |e 1 |b 18 |c 11 |h 63-69 |
author_variant |
h h hh d r dr t g tg m m y mmy |
---|---|
matchkey_str |
article:16157591:2004----::nacdmlspoutobbclusbiiuigdaep |
hierarchy_sort_str |
2004 |
publishDate |
2004 |
allfields |
10.1007/s00449-004-0391-z doi (DE-627)OLC2106609590 (DE-He213)s00449-004-0391-z-p DE-627 ger DE-627 rakwb eng 660 VZ 570 690 540 VZ 12 ssgn Huang, Hanjing verfasserin aut Enhanced amylase production by Bacillus subtilis using a dual exponential feeding strategy 2004 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag 2004 Abstract A recombinant Bacillus subtilis strain (ATCC 31784) haboring the plasmid pC194 with a thermostable α-amylase gene was cultured in a 22-l B. Braun Biostat C fermenter. Traditional batch operations suffer from low cell mass and protein productions because a high initial glucose concentration causes substrate inhibition and also product inhibition due to acetate accumulation. An exponential fed-batch strategy to prevent these inhibitions was developed in this work. The host strain is auxotrophic for phenylalanine, tyrosine and tryptophan. Due to low solubilities of tyrosine and tryptophan in the feed stream, tyrosine and tryptophan were dissolved separately in ammonia water to form a second feed stream. By dual feeding both streams at different exponential feed rates, a high cell density of 17.6 g/l and a final α-amylase activity of 41.4 U/ml and the overall biomass yield of 0.39 g cell/g glucose were achieved. Fermentation Dual feeding Exponential fed-batch culture Ridgway, Darin aut Gu, Tingyue aut Moo-Young, Murray aut Enthalten in Bioprocess and biosystems engineering Springer-Verlag, 2001 27(2004), 1 vom: 18. Nov., Seite 63-69 (DE-627)333469763 (DE-600)2056063-1 (DE-576)094533709 1615-7591 nnns volume:27 year:2004 number:1 day:18 month:11 pages:63-69 https://doi.org/10.1007/s00449-004-0391-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-ARC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_11 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_70 GBV_ILN_2006 GBV_ILN_2014 GBV_ILN_2018 GBV_ILN_4046 GBV_ILN_4277 GBV_ILN_4307 AR 27 2004 1 18 11 63-69 |
spelling |
10.1007/s00449-004-0391-z doi (DE-627)OLC2106609590 (DE-He213)s00449-004-0391-z-p DE-627 ger DE-627 rakwb eng 660 VZ 570 690 540 VZ 12 ssgn Huang, Hanjing verfasserin aut Enhanced amylase production by Bacillus subtilis using a dual exponential feeding strategy 2004 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag 2004 Abstract A recombinant Bacillus subtilis strain (ATCC 31784) haboring the plasmid pC194 with a thermostable α-amylase gene was cultured in a 22-l B. Braun Biostat C fermenter. Traditional batch operations suffer from low cell mass and protein productions because a high initial glucose concentration causes substrate inhibition and also product inhibition due to acetate accumulation. An exponential fed-batch strategy to prevent these inhibitions was developed in this work. The host strain is auxotrophic for phenylalanine, tyrosine and tryptophan. Due to low solubilities of tyrosine and tryptophan in the feed stream, tyrosine and tryptophan were dissolved separately in ammonia water to form a second feed stream. By dual feeding both streams at different exponential feed rates, a high cell density of 17.6 g/l and a final α-amylase activity of 41.4 U/ml and the overall biomass yield of 0.39 g cell/g glucose were achieved. Fermentation Dual feeding Exponential fed-batch culture Ridgway, Darin aut Gu, Tingyue aut Moo-Young, Murray aut Enthalten in Bioprocess and biosystems engineering Springer-Verlag, 2001 27(2004), 1 vom: 18. Nov., Seite 63-69 (DE-627)333469763 (DE-600)2056063-1 (DE-576)094533709 1615-7591 nnns volume:27 year:2004 number:1 day:18 month:11 pages:63-69 https://doi.org/10.1007/s00449-004-0391-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-ARC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_11 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_70 GBV_ILN_2006 GBV_ILN_2014 GBV_ILN_2018 GBV_ILN_4046 GBV_ILN_4277 GBV_ILN_4307 AR 27 2004 1 18 11 63-69 |
allfields_unstemmed |
10.1007/s00449-004-0391-z doi (DE-627)OLC2106609590 (DE-He213)s00449-004-0391-z-p DE-627 ger DE-627 rakwb eng 660 VZ 570 690 540 VZ 12 ssgn Huang, Hanjing verfasserin aut Enhanced amylase production by Bacillus subtilis using a dual exponential feeding strategy 2004 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag 2004 Abstract A recombinant Bacillus subtilis strain (ATCC 31784) haboring the plasmid pC194 with a thermostable α-amylase gene was cultured in a 22-l B. Braun Biostat C fermenter. Traditional batch operations suffer from low cell mass and protein productions because a high initial glucose concentration causes substrate inhibition and also product inhibition due to acetate accumulation. An exponential fed-batch strategy to prevent these inhibitions was developed in this work. The host strain is auxotrophic for phenylalanine, tyrosine and tryptophan. Due to low solubilities of tyrosine and tryptophan in the feed stream, tyrosine and tryptophan were dissolved separately in ammonia water to form a second feed stream. By dual feeding both streams at different exponential feed rates, a high cell density of 17.6 g/l and a final α-amylase activity of 41.4 U/ml and the overall biomass yield of 0.39 g cell/g glucose were achieved. Fermentation Dual feeding Exponential fed-batch culture Ridgway, Darin aut Gu, Tingyue aut Moo-Young, Murray aut Enthalten in Bioprocess and biosystems engineering Springer-Verlag, 2001 27(2004), 1 vom: 18. Nov., Seite 63-69 (DE-627)333469763 (DE-600)2056063-1 (DE-576)094533709 1615-7591 nnns volume:27 year:2004 number:1 day:18 month:11 pages:63-69 https://doi.org/10.1007/s00449-004-0391-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-ARC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_11 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_70 GBV_ILN_2006 GBV_ILN_2014 GBV_ILN_2018 GBV_ILN_4046 GBV_ILN_4277 GBV_ILN_4307 AR 27 2004 1 18 11 63-69 |
allfieldsGer |
10.1007/s00449-004-0391-z doi (DE-627)OLC2106609590 (DE-He213)s00449-004-0391-z-p DE-627 ger DE-627 rakwb eng 660 VZ 570 690 540 VZ 12 ssgn Huang, Hanjing verfasserin aut Enhanced amylase production by Bacillus subtilis using a dual exponential feeding strategy 2004 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag 2004 Abstract A recombinant Bacillus subtilis strain (ATCC 31784) haboring the plasmid pC194 with a thermostable α-amylase gene was cultured in a 22-l B. Braun Biostat C fermenter. Traditional batch operations suffer from low cell mass and protein productions because a high initial glucose concentration causes substrate inhibition and also product inhibition due to acetate accumulation. An exponential fed-batch strategy to prevent these inhibitions was developed in this work. The host strain is auxotrophic for phenylalanine, tyrosine and tryptophan. Due to low solubilities of tyrosine and tryptophan in the feed stream, tyrosine and tryptophan were dissolved separately in ammonia water to form a second feed stream. By dual feeding both streams at different exponential feed rates, a high cell density of 17.6 g/l and a final α-amylase activity of 41.4 U/ml and the overall biomass yield of 0.39 g cell/g glucose were achieved. Fermentation Dual feeding Exponential fed-batch culture Ridgway, Darin aut Gu, Tingyue aut Moo-Young, Murray aut Enthalten in Bioprocess and biosystems engineering Springer-Verlag, 2001 27(2004), 1 vom: 18. Nov., Seite 63-69 (DE-627)333469763 (DE-600)2056063-1 (DE-576)094533709 1615-7591 nnns volume:27 year:2004 number:1 day:18 month:11 pages:63-69 https://doi.org/10.1007/s00449-004-0391-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-ARC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_11 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_70 GBV_ILN_2006 GBV_ILN_2014 GBV_ILN_2018 GBV_ILN_4046 GBV_ILN_4277 GBV_ILN_4307 AR 27 2004 1 18 11 63-69 |
allfieldsSound |
10.1007/s00449-004-0391-z doi (DE-627)OLC2106609590 (DE-He213)s00449-004-0391-z-p DE-627 ger DE-627 rakwb eng 660 VZ 570 690 540 VZ 12 ssgn Huang, Hanjing verfasserin aut Enhanced amylase production by Bacillus subtilis using a dual exponential feeding strategy 2004 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag 2004 Abstract A recombinant Bacillus subtilis strain (ATCC 31784) haboring the plasmid pC194 with a thermostable α-amylase gene was cultured in a 22-l B. Braun Biostat C fermenter. Traditional batch operations suffer from low cell mass and protein productions because a high initial glucose concentration causes substrate inhibition and also product inhibition due to acetate accumulation. An exponential fed-batch strategy to prevent these inhibitions was developed in this work. The host strain is auxotrophic for phenylalanine, tyrosine and tryptophan. Due to low solubilities of tyrosine and tryptophan in the feed stream, tyrosine and tryptophan were dissolved separately in ammonia water to form a second feed stream. By dual feeding both streams at different exponential feed rates, a high cell density of 17.6 g/l and a final α-amylase activity of 41.4 U/ml and the overall biomass yield of 0.39 g cell/g glucose were achieved. Fermentation Dual feeding Exponential fed-batch culture Ridgway, Darin aut Gu, Tingyue aut Moo-Young, Murray aut Enthalten in Bioprocess and biosystems engineering Springer-Verlag, 2001 27(2004), 1 vom: 18. Nov., Seite 63-69 (DE-627)333469763 (DE-600)2056063-1 (DE-576)094533709 1615-7591 nnns volume:27 year:2004 number:1 day:18 month:11 pages:63-69 https://doi.org/10.1007/s00449-004-0391-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-ARC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_11 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_70 GBV_ILN_2006 GBV_ILN_2014 GBV_ILN_2018 GBV_ILN_4046 GBV_ILN_4277 GBV_ILN_4307 AR 27 2004 1 18 11 63-69 |
language |
English |
source |
Enthalten in Bioprocess and biosystems engineering 27(2004), 1 vom: 18. Nov., Seite 63-69 volume:27 year:2004 number:1 day:18 month:11 pages:63-69 |
sourceStr |
Enthalten in Bioprocess and biosystems engineering 27(2004), 1 vom: 18. Nov., Seite 63-69 volume:27 year:2004 number:1 day:18 month:11 pages:63-69 |
format_phy_str_mv |
Article |
institution |
findex.gbv.de |
topic_facet |
Fermentation Dual feeding Exponential fed-batch culture |
dewey-raw |
660 |
isfreeaccess_bool |
false |
container_title |
Bioprocess and biosystems engineering |
authorswithroles_txt_mv |
Huang, Hanjing @@aut@@ Ridgway, Darin @@aut@@ Gu, Tingyue @@aut@@ Moo-Young, Murray @@aut@@ |
publishDateDaySort_date |
2004-11-18T00:00:00Z |
hierarchy_top_id |
333469763 |
dewey-sort |
3660 |
id |
OLC2106609590 |
language_de |
englisch |
fullrecord |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000naa a22002652 4500</leader><controlfield tag="001">OLC2106609590</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230403073226.0</controlfield><controlfield tag="007">tu</controlfield><controlfield tag="008">230403s2004 xx ||||| 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/s00449-004-0391-z</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)OLC2106609590</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-He213)s00449-004-0391-z-p</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">660</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">570</subfield><subfield code="a">690</subfield><subfield code="a">540</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">12</subfield><subfield code="2">ssgn</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Huang, Hanjing</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Enhanced amylase production by Bacillus subtilis using a dual exponential feeding strategy</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2004</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">ohne Hilfsmittel zu benutzen</subfield><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Band</subfield><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">© Springer-Verlag 2004</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract A recombinant Bacillus subtilis strain (ATCC 31784) haboring the plasmid pC194 with a thermostable α-amylase gene was cultured in a 22-l B. Braun Biostat C fermenter. Traditional batch operations suffer from low cell mass and protein productions because a high initial glucose concentration causes substrate inhibition and also product inhibition due to acetate accumulation. An exponential fed-batch strategy to prevent these inhibitions was developed in this work. The host strain is auxotrophic for phenylalanine, tyrosine and tryptophan. Due to low solubilities of tyrosine and tryptophan in the feed stream, tyrosine and tryptophan were dissolved separately in ammonia water to form a second feed stream. By dual feeding both streams at different exponential feed rates, a high cell density of 17.6 g/l and a final α-amylase activity of 41.4 U/ml and the overall biomass yield of 0.39 g cell/g glucose were achieved.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Fermentation</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Dual feeding</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Exponential fed-batch culture</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Ridgway, Darin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Gu, Tingyue</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Moo-Young, Murray</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Bioprocess and biosystems engineering</subfield><subfield code="d">Springer-Verlag, 2001</subfield><subfield code="g">27(2004), 1 vom: 18. Nov., Seite 63-69</subfield><subfield code="w">(DE-627)333469763</subfield><subfield code="w">(DE-600)2056063-1</subfield><subfield code="w">(DE-576)094533709</subfield><subfield code="x">1615-7591</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:27</subfield><subfield code="g">year:2004</subfield><subfield code="g">number:1</subfield><subfield code="g">day:18</subfield><subfield code="g">month:11</subfield><subfield code="g">pages:63-69</subfield></datafield><datafield tag="856" ind1="4" ind2="1"><subfield code="u">https://doi.org/10.1007/s00449-004-0391-z</subfield><subfield code="z">lizenzpflichtig</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_OLC</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-UMW</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-ARC</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-TEC</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-CHE</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_11</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_23</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_24</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_31</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_32</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_70</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2006</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2014</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2018</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4046</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4277</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4307</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">27</subfield><subfield code="j">2004</subfield><subfield code="e">1</subfield><subfield code="b">18</subfield><subfield code="c">11</subfield><subfield code="h">63-69</subfield></datafield></record></collection>
|
author |
Huang, Hanjing |
spellingShingle |
Huang, Hanjing ddc 660 ddc 570 ssgn 12 misc Fermentation misc Dual feeding misc Exponential fed-batch culture Enhanced amylase production by Bacillus subtilis using a dual exponential feeding strategy |
authorStr |
Huang, Hanjing |
ppnlink_with_tag_str_mv |
@@773@@(DE-627)333469763 |
format |
Article |
dewey-ones |
660 - Chemical engineering 570 - Life sciences; biology 690 - Buildings 540 - Chemistry & allied sciences |
delete_txt_mv |
keep |
author_role |
aut aut aut aut |
collection |
OLC |
remote_str |
false |
illustrated |
Not Illustrated |
issn |
1615-7591 |
topic_title |
660 VZ 570 690 540 VZ 12 ssgn Enhanced amylase production by Bacillus subtilis using a dual exponential feeding strategy Fermentation Dual feeding Exponential fed-batch culture |
topic |
ddc 660 ddc 570 ssgn 12 misc Fermentation misc Dual feeding misc Exponential fed-batch culture |
topic_unstemmed |
ddc 660 ddc 570 ssgn 12 misc Fermentation misc Dual feeding misc Exponential fed-batch culture |
topic_browse |
ddc 660 ddc 570 ssgn 12 misc Fermentation misc Dual feeding misc Exponential fed-batch culture |
format_facet |
Aufsätze Gedruckte Aufsätze |
format_main_str_mv |
Text Zeitschrift/Artikel |
carriertype_str_mv |
nc |
hierarchy_parent_title |
Bioprocess and biosystems engineering |
hierarchy_parent_id |
333469763 |
dewey-tens |
660 - Chemical engineering 570 - Life sciences; biology 690 - Building & construction 540 - Chemistry |
hierarchy_top_title |
Bioprocess and biosystems engineering |
isfreeaccess_txt |
false |
familylinks_str_mv |
(DE-627)333469763 (DE-600)2056063-1 (DE-576)094533709 |
title |
Enhanced amylase production by Bacillus subtilis using a dual exponential feeding strategy |
ctrlnum |
(DE-627)OLC2106609590 (DE-He213)s00449-004-0391-z-p |
title_full |
Enhanced amylase production by Bacillus subtilis using a dual exponential feeding strategy |
author_sort |
Huang, Hanjing |
journal |
Bioprocess and biosystems engineering |
journalStr |
Bioprocess and biosystems engineering |
lang_code |
eng |
isOA_bool |
false |
dewey-hundreds |
600 - Technology 500 - Science |
recordtype |
marc |
publishDateSort |
2004 |
contenttype_str_mv |
txt |
container_start_page |
63 |
author_browse |
Huang, Hanjing Ridgway, Darin Gu, Tingyue Moo-Young, Murray |
container_volume |
27 |
class |
660 VZ 570 690 540 VZ 12 ssgn |
format_se |
Aufsätze |
author-letter |
Huang, Hanjing |
doi_str_mv |
10.1007/s00449-004-0391-z |
dewey-full |
660 570 690 540 |
title_sort |
enhanced amylase production by bacillus subtilis using a dual exponential feeding strategy |
title_auth |
Enhanced amylase production by Bacillus subtilis using a dual exponential feeding strategy |
abstract |
Abstract A recombinant Bacillus subtilis strain (ATCC 31784) haboring the plasmid pC194 with a thermostable α-amylase gene was cultured in a 22-l B. Braun Biostat C fermenter. Traditional batch operations suffer from low cell mass and protein productions because a high initial glucose concentration causes substrate inhibition and also product inhibition due to acetate accumulation. An exponential fed-batch strategy to prevent these inhibitions was developed in this work. The host strain is auxotrophic for phenylalanine, tyrosine and tryptophan. Due to low solubilities of tyrosine and tryptophan in the feed stream, tyrosine and tryptophan were dissolved separately in ammonia water to form a second feed stream. By dual feeding both streams at different exponential feed rates, a high cell density of 17.6 g/l and a final α-amylase activity of 41.4 U/ml and the overall biomass yield of 0.39 g cell/g glucose were achieved. © Springer-Verlag 2004 |
abstractGer |
Abstract A recombinant Bacillus subtilis strain (ATCC 31784) haboring the plasmid pC194 with a thermostable α-amylase gene was cultured in a 22-l B. Braun Biostat C fermenter. Traditional batch operations suffer from low cell mass and protein productions because a high initial glucose concentration causes substrate inhibition and also product inhibition due to acetate accumulation. An exponential fed-batch strategy to prevent these inhibitions was developed in this work. The host strain is auxotrophic for phenylalanine, tyrosine and tryptophan. Due to low solubilities of tyrosine and tryptophan in the feed stream, tyrosine and tryptophan were dissolved separately in ammonia water to form a second feed stream. By dual feeding both streams at different exponential feed rates, a high cell density of 17.6 g/l and a final α-amylase activity of 41.4 U/ml and the overall biomass yield of 0.39 g cell/g glucose were achieved. © Springer-Verlag 2004 |
abstract_unstemmed |
Abstract A recombinant Bacillus subtilis strain (ATCC 31784) haboring the plasmid pC194 with a thermostable α-amylase gene was cultured in a 22-l B. Braun Biostat C fermenter. Traditional batch operations suffer from low cell mass and protein productions because a high initial glucose concentration causes substrate inhibition and also product inhibition due to acetate accumulation. An exponential fed-batch strategy to prevent these inhibitions was developed in this work. The host strain is auxotrophic for phenylalanine, tyrosine and tryptophan. Due to low solubilities of tyrosine and tryptophan in the feed stream, tyrosine and tryptophan were dissolved separately in ammonia water to form a second feed stream. By dual feeding both streams at different exponential feed rates, a high cell density of 17.6 g/l and a final α-amylase activity of 41.4 U/ml and the overall biomass yield of 0.39 g cell/g glucose were achieved. © Springer-Verlag 2004 |
collection_details |
GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-ARC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_11 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_70 GBV_ILN_2006 GBV_ILN_2014 GBV_ILN_2018 GBV_ILN_4046 GBV_ILN_4277 GBV_ILN_4307 |
container_issue |
1 |
title_short |
Enhanced amylase production by Bacillus subtilis using a dual exponential feeding strategy |
url |
https://doi.org/10.1007/s00449-004-0391-z |
remote_bool |
false |
author2 |
Ridgway, Darin Gu, Tingyue Moo-Young, Murray |
author2Str |
Ridgway, Darin Gu, Tingyue Moo-Young, Murray |
ppnlink |
333469763 |
mediatype_str_mv |
n |
isOA_txt |
false |
hochschulschrift_bool |
false |
doi_str |
10.1007/s00449-004-0391-z |
up_date |
2024-07-04T07:05:03.313Z |
_version_ |
1803631149246840832 |
fullrecord_marcxml |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000naa a22002652 4500</leader><controlfield tag="001">OLC2106609590</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230403073226.0</controlfield><controlfield tag="007">tu</controlfield><controlfield tag="008">230403s2004 xx ||||| 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/s00449-004-0391-z</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)OLC2106609590</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-He213)s00449-004-0391-z-p</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">660</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">570</subfield><subfield code="a">690</subfield><subfield code="a">540</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">12</subfield><subfield code="2">ssgn</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Huang, Hanjing</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Enhanced amylase production by Bacillus subtilis using a dual exponential feeding strategy</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2004</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">ohne Hilfsmittel zu benutzen</subfield><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Band</subfield><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">© Springer-Verlag 2004</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract A recombinant Bacillus subtilis strain (ATCC 31784) haboring the plasmid pC194 with a thermostable α-amylase gene was cultured in a 22-l B. Braun Biostat C fermenter. Traditional batch operations suffer from low cell mass and protein productions because a high initial glucose concentration causes substrate inhibition and also product inhibition due to acetate accumulation. An exponential fed-batch strategy to prevent these inhibitions was developed in this work. The host strain is auxotrophic for phenylalanine, tyrosine and tryptophan. Due to low solubilities of tyrosine and tryptophan in the feed stream, tyrosine and tryptophan were dissolved separately in ammonia water to form a second feed stream. By dual feeding both streams at different exponential feed rates, a high cell density of 17.6 g/l and a final α-amylase activity of 41.4 U/ml and the overall biomass yield of 0.39 g cell/g glucose were achieved.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Fermentation</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Dual feeding</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Exponential fed-batch culture</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Ridgway, Darin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Gu, Tingyue</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Moo-Young, Murray</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Bioprocess and biosystems engineering</subfield><subfield code="d">Springer-Verlag, 2001</subfield><subfield code="g">27(2004), 1 vom: 18. Nov., Seite 63-69</subfield><subfield code="w">(DE-627)333469763</subfield><subfield code="w">(DE-600)2056063-1</subfield><subfield code="w">(DE-576)094533709</subfield><subfield code="x">1615-7591</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:27</subfield><subfield code="g">year:2004</subfield><subfield code="g">number:1</subfield><subfield code="g">day:18</subfield><subfield code="g">month:11</subfield><subfield code="g">pages:63-69</subfield></datafield><datafield tag="856" ind1="4" ind2="1"><subfield code="u">https://doi.org/10.1007/s00449-004-0391-z</subfield><subfield code="z">lizenzpflichtig</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_OLC</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-UMW</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-ARC</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-TEC</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-CHE</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_11</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_23</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_24</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_31</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_32</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_70</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2006</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2014</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2018</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4046</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4277</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4307</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">27</subfield><subfield code="j">2004</subfield><subfield code="e">1</subfield><subfield code="b">18</subfield><subfield code="c">11</subfield><subfield code="h">63-69</subfield></datafield></record></collection>
|
score |
7.3982754 |