Effective and stable porcine interferon-α production by Pichia pastoris fed-batch cultivation with multi-variables clustering and analysis
Abstract Efficient porcine interferon-α (pIFN-α) expression in high density recombinant Pichia pastoris cultivation was achieved in a 5 l bench-scaled bioreactor. The results indicated that a high and stable oxygen uptake rate (OUR) during induction phase was closely related with pIFN-α production e...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Yu, Ruisong [verfasserIn] |
|---|
| Format: |
Artikel |
|---|---|
| Sprache: |
Englisch |
| Erschienen: |
2009 |
|---|
| Schlagwörter: |
|---|
| Anmerkung: |
© Springer-Verlag 2009 |
|---|
| Übergeordnetes Werk: |
Enthalten in: Bioprocess and biosystems engineering - Springer-Verlag, 2001, 33(2009), 4 vom: 02. Aug., Seite 473-483 |
|---|---|
| Übergeordnetes Werk: |
volume:33 ; year:2009 ; number:4 ; day:02 ; month:08 ; pages:473-483 |
| Links: |
|---|
| DOI / URN: |
10.1007/s00449-009-0356-3 |
|---|
| Katalog-ID: |
OLC2106617062 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | OLC2106617062 | ||
| 003 | DE-627 | ||
| 005 | 20230403073304.0 | ||
| 007 | tu | ||
| 008 | 230403s2009 xx ||||| 00| ||eng c | ||
| 024 | 7 | |a 10.1007/s00449-009-0356-3 |2 doi | |
| 035 | |a (DE-627)OLC2106617062 | ||
| 035 | |a (DE-He213)s00449-009-0356-3-p | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 082 | 0 | 4 | |a 660 |q VZ |
| 082 | 0 | 4 | |a 570 |a 690 |a 540 |q VZ |
| 084 | |a 12 |2 ssgn | ||
| 100 | 1 | |a Yu, Ruisong |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Effective and stable porcine interferon-α production by Pichia pastoris fed-batch cultivation with multi-variables clustering and analysis |
| 264 | 1 | |c 2009 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a © Springer-Verlag 2009 | ||
| 520 | |a Abstract Efficient porcine interferon-α (pIFN-α) expression in high density recombinant Pichia pastoris cultivation was achieved in a 5 l bench-scaled bioreactor. The results indicated that a high and stable oxygen uptake rate (OUR) during induction phase was closely related with pIFN-α production efficiency. The multi-variables clustering and analysis results showed that the achievement of a high and stable OUR relied on a higher glycerol consumption rate during fed-batch culture phase and a moderate methanol level (around 10 g/l) during induction phase. In the high and stable OUR environments (200–300 mmol/l/h), the highest pIFN-α antiviral activity could reach a level of 6.7 × $ 10^{6} $ IU/ml, which was more than 10–300-folds higher than those obtained at lower OUR (80–200 mmol/l/h) using the same bioreactor and those obtained in shaking flasks. Clustering and analysis of the specific growth and glycerol consumption rates data during culture phase could detect the ill fermentation state at early stage, potentially provided a simple and effective fault alarming/diagnosis method for the achievement of stable pIFN-α production. | ||
| 650 | 4 | |a Fed-batch culture | |
| 650 | 4 | |a Multi-variables clustering | |
| 650 | 4 | |a Oxygen uptake rate (OUR) | |
| 650 | 4 | |a Porcine interferon-α expression | |
| 700 | 1 | |a Dong, Shijuan |4 aut | |
| 700 | 1 | |a Zhu, Yumin |4 aut | |
| 700 | 1 | |a Jin, Hu |4 aut | |
| 700 | 1 | |a Gao, Mingjie |4 aut | |
| 700 | 1 | |a Duan, Zuoying |4 aut | |
| 700 | 1 | |a Zheng, Zhiyong |4 aut | |
| 700 | 1 | |a Shi, Zhongping |4 aut | |
| 700 | 1 | |a Li, Zhen |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Bioprocess and biosystems engineering |d Springer-Verlag, 2001 |g 33(2009), 4 vom: 02. Aug., Seite 473-483 |w (DE-627)333469763 |w (DE-600)2056063-1 |w (DE-576)094533709 |x 1615-7591 |7 nnns |
| 773 | 1 | 8 | |g volume:33 |g year:2009 |g number:4 |g day:02 |g month:08 |g pages:473-483 |
| 856 | 4 | 1 | |u https://doi.org/10.1007/s00449-009-0356-3 |z lizenzpflichtig |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a SYSFLAG_A | ||
| 912 | |a GBV_OLC | ||
| 912 | |a SSG-OLC-UMW | ||
| 912 | |a SSG-OLC-ARC | ||
| 912 | |a SSG-OLC-TEC | ||
| 912 | |a SSG-OLC-CHE | ||
| 912 | |a GBV_ILN_23 | ||
| 912 | |a GBV_ILN_24 | ||
| 912 | |a GBV_ILN_31 | ||
| 912 | |a GBV_ILN_32 | ||
| 912 | |a GBV_ILN_70 | ||
| 912 | |a GBV_ILN_2006 | ||
| 912 | |a GBV_ILN_2018 | ||
| 912 | |a GBV_ILN_4046 | ||
| 912 | |a GBV_ILN_4277 | ||
| 912 | |a GBV_ILN_4307 | ||
| 951 | |a AR | ||
| 952 | |d 33 |j 2009 |e 4 |b 02 |c 08 |h 473-483 | ||
| author_variant |
r y ry s d sd y z yz h j hj m g mg z d zd z z zz z s zs z l zl |
|---|---|
| matchkey_str |
article:16157591:2009----::fetvadtbeocnitreopoutobpcipsoifdaccliainihu |
| hierarchy_sort_str |
2009 |
| publishDate |
2009 |
| allfields |
10.1007/s00449-009-0356-3 doi (DE-627)OLC2106617062 (DE-He213)s00449-009-0356-3-p DE-627 ger DE-627 rakwb eng 660 VZ 570 690 540 VZ 12 ssgn Yu, Ruisong verfasserin aut Effective and stable porcine interferon-α production by Pichia pastoris fed-batch cultivation with multi-variables clustering and analysis 2009 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag 2009 Abstract Efficient porcine interferon-α (pIFN-α) expression in high density recombinant Pichia pastoris cultivation was achieved in a 5 l bench-scaled bioreactor. The results indicated that a high and stable oxygen uptake rate (OUR) during induction phase was closely related with pIFN-α production efficiency. The multi-variables clustering and analysis results showed that the achievement of a high and stable OUR relied on a higher glycerol consumption rate during fed-batch culture phase and a moderate methanol level (around 10 g/l) during induction phase. In the high and stable OUR environments (200–300 mmol/l/h), the highest pIFN-α antiviral activity could reach a level of 6.7 × $ 10^{6} $ IU/ml, which was more than 10–300-folds higher than those obtained at lower OUR (80–200 mmol/l/h) using the same bioreactor and those obtained in shaking flasks. Clustering and analysis of the specific growth and glycerol consumption rates data during culture phase could detect the ill fermentation state at early stage, potentially provided a simple and effective fault alarming/diagnosis method for the achievement of stable pIFN-α production. Fed-batch culture Multi-variables clustering Oxygen uptake rate (OUR) Porcine interferon-α expression Dong, Shijuan aut Zhu, Yumin aut Jin, Hu aut Gao, Mingjie aut Duan, Zuoying aut Zheng, Zhiyong aut Shi, Zhongping aut Li, Zhen aut Enthalten in Bioprocess and biosystems engineering Springer-Verlag, 2001 33(2009), 4 vom: 02. Aug., Seite 473-483 (DE-627)333469763 (DE-600)2056063-1 (DE-576)094533709 1615-7591 nnns volume:33 year:2009 number:4 day:02 month:08 pages:473-483 https://doi.org/10.1007/s00449-009-0356-3 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-ARC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_70 GBV_ILN_2006 GBV_ILN_2018 GBV_ILN_4046 GBV_ILN_4277 GBV_ILN_4307 AR 33 2009 4 02 08 473-483 |
| spelling |
10.1007/s00449-009-0356-3 doi (DE-627)OLC2106617062 (DE-He213)s00449-009-0356-3-p DE-627 ger DE-627 rakwb eng 660 VZ 570 690 540 VZ 12 ssgn Yu, Ruisong verfasserin aut Effective and stable porcine interferon-α production by Pichia pastoris fed-batch cultivation with multi-variables clustering and analysis 2009 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag 2009 Abstract Efficient porcine interferon-α (pIFN-α) expression in high density recombinant Pichia pastoris cultivation was achieved in a 5 l bench-scaled bioreactor. The results indicated that a high and stable oxygen uptake rate (OUR) during induction phase was closely related with pIFN-α production efficiency. The multi-variables clustering and analysis results showed that the achievement of a high and stable OUR relied on a higher glycerol consumption rate during fed-batch culture phase and a moderate methanol level (around 10 g/l) during induction phase. In the high and stable OUR environments (200–300 mmol/l/h), the highest pIFN-α antiviral activity could reach a level of 6.7 × $ 10^{6} $ IU/ml, which was more than 10–300-folds higher than those obtained at lower OUR (80–200 mmol/l/h) using the same bioreactor and those obtained in shaking flasks. Clustering and analysis of the specific growth and glycerol consumption rates data during culture phase could detect the ill fermentation state at early stage, potentially provided a simple and effective fault alarming/diagnosis method for the achievement of stable pIFN-α production. Fed-batch culture Multi-variables clustering Oxygen uptake rate (OUR) Porcine interferon-α expression Dong, Shijuan aut Zhu, Yumin aut Jin, Hu aut Gao, Mingjie aut Duan, Zuoying aut Zheng, Zhiyong aut Shi, Zhongping aut Li, Zhen aut Enthalten in Bioprocess and biosystems engineering Springer-Verlag, 2001 33(2009), 4 vom: 02. Aug., Seite 473-483 (DE-627)333469763 (DE-600)2056063-1 (DE-576)094533709 1615-7591 nnns volume:33 year:2009 number:4 day:02 month:08 pages:473-483 https://doi.org/10.1007/s00449-009-0356-3 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-ARC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_70 GBV_ILN_2006 GBV_ILN_2018 GBV_ILN_4046 GBV_ILN_4277 GBV_ILN_4307 AR 33 2009 4 02 08 473-483 |
| allfields_unstemmed |
10.1007/s00449-009-0356-3 doi (DE-627)OLC2106617062 (DE-He213)s00449-009-0356-3-p DE-627 ger DE-627 rakwb eng 660 VZ 570 690 540 VZ 12 ssgn Yu, Ruisong verfasserin aut Effective and stable porcine interferon-α production by Pichia pastoris fed-batch cultivation with multi-variables clustering and analysis 2009 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag 2009 Abstract Efficient porcine interferon-α (pIFN-α) expression in high density recombinant Pichia pastoris cultivation was achieved in a 5 l bench-scaled bioreactor. The results indicated that a high and stable oxygen uptake rate (OUR) during induction phase was closely related with pIFN-α production efficiency. The multi-variables clustering and analysis results showed that the achievement of a high and stable OUR relied on a higher glycerol consumption rate during fed-batch culture phase and a moderate methanol level (around 10 g/l) during induction phase. In the high and stable OUR environments (200–300 mmol/l/h), the highest pIFN-α antiviral activity could reach a level of 6.7 × $ 10^{6} $ IU/ml, which was more than 10–300-folds higher than those obtained at lower OUR (80–200 mmol/l/h) using the same bioreactor and those obtained in shaking flasks. Clustering and analysis of the specific growth and glycerol consumption rates data during culture phase could detect the ill fermentation state at early stage, potentially provided a simple and effective fault alarming/diagnosis method for the achievement of stable pIFN-α production. Fed-batch culture Multi-variables clustering Oxygen uptake rate (OUR) Porcine interferon-α expression Dong, Shijuan aut Zhu, Yumin aut Jin, Hu aut Gao, Mingjie aut Duan, Zuoying aut Zheng, Zhiyong aut Shi, Zhongping aut Li, Zhen aut Enthalten in Bioprocess and biosystems engineering Springer-Verlag, 2001 33(2009), 4 vom: 02. Aug., Seite 473-483 (DE-627)333469763 (DE-600)2056063-1 (DE-576)094533709 1615-7591 nnns volume:33 year:2009 number:4 day:02 month:08 pages:473-483 https://doi.org/10.1007/s00449-009-0356-3 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-ARC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_70 GBV_ILN_2006 GBV_ILN_2018 GBV_ILN_4046 GBV_ILN_4277 GBV_ILN_4307 AR 33 2009 4 02 08 473-483 |
| allfieldsGer |
10.1007/s00449-009-0356-3 doi (DE-627)OLC2106617062 (DE-He213)s00449-009-0356-3-p DE-627 ger DE-627 rakwb eng 660 VZ 570 690 540 VZ 12 ssgn Yu, Ruisong verfasserin aut Effective and stable porcine interferon-α production by Pichia pastoris fed-batch cultivation with multi-variables clustering and analysis 2009 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag 2009 Abstract Efficient porcine interferon-α (pIFN-α) expression in high density recombinant Pichia pastoris cultivation was achieved in a 5 l bench-scaled bioreactor. The results indicated that a high and stable oxygen uptake rate (OUR) during induction phase was closely related with pIFN-α production efficiency. The multi-variables clustering and analysis results showed that the achievement of a high and stable OUR relied on a higher glycerol consumption rate during fed-batch culture phase and a moderate methanol level (around 10 g/l) during induction phase. In the high and stable OUR environments (200–300 mmol/l/h), the highest pIFN-α antiviral activity could reach a level of 6.7 × $ 10^{6} $ IU/ml, which was more than 10–300-folds higher than those obtained at lower OUR (80–200 mmol/l/h) using the same bioreactor and those obtained in shaking flasks. Clustering and analysis of the specific growth and glycerol consumption rates data during culture phase could detect the ill fermentation state at early stage, potentially provided a simple and effective fault alarming/diagnosis method for the achievement of stable pIFN-α production. Fed-batch culture Multi-variables clustering Oxygen uptake rate (OUR) Porcine interferon-α expression Dong, Shijuan aut Zhu, Yumin aut Jin, Hu aut Gao, Mingjie aut Duan, Zuoying aut Zheng, Zhiyong aut Shi, Zhongping aut Li, Zhen aut Enthalten in Bioprocess and biosystems engineering Springer-Verlag, 2001 33(2009), 4 vom: 02. Aug., Seite 473-483 (DE-627)333469763 (DE-600)2056063-1 (DE-576)094533709 1615-7591 nnns volume:33 year:2009 number:4 day:02 month:08 pages:473-483 https://doi.org/10.1007/s00449-009-0356-3 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-ARC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_70 GBV_ILN_2006 GBV_ILN_2018 GBV_ILN_4046 GBV_ILN_4277 GBV_ILN_4307 AR 33 2009 4 02 08 473-483 |
| allfieldsSound |
10.1007/s00449-009-0356-3 doi (DE-627)OLC2106617062 (DE-He213)s00449-009-0356-3-p DE-627 ger DE-627 rakwb eng 660 VZ 570 690 540 VZ 12 ssgn Yu, Ruisong verfasserin aut Effective and stable porcine interferon-α production by Pichia pastoris fed-batch cultivation with multi-variables clustering and analysis 2009 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag 2009 Abstract Efficient porcine interferon-α (pIFN-α) expression in high density recombinant Pichia pastoris cultivation was achieved in a 5 l bench-scaled bioreactor. The results indicated that a high and stable oxygen uptake rate (OUR) during induction phase was closely related with pIFN-α production efficiency. The multi-variables clustering and analysis results showed that the achievement of a high and stable OUR relied on a higher glycerol consumption rate during fed-batch culture phase and a moderate methanol level (around 10 g/l) during induction phase. In the high and stable OUR environments (200–300 mmol/l/h), the highest pIFN-α antiviral activity could reach a level of 6.7 × $ 10^{6} $ IU/ml, which was more than 10–300-folds higher than those obtained at lower OUR (80–200 mmol/l/h) using the same bioreactor and those obtained in shaking flasks. Clustering and analysis of the specific growth and glycerol consumption rates data during culture phase could detect the ill fermentation state at early stage, potentially provided a simple and effective fault alarming/diagnosis method for the achievement of stable pIFN-α production. Fed-batch culture Multi-variables clustering Oxygen uptake rate (OUR) Porcine interferon-α expression Dong, Shijuan aut Zhu, Yumin aut Jin, Hu aut Gao, Mingjie aut Duan, Zuoying aut Zheng, Zhiyong aut Shi, Zhongping aut Li, Zhen aut Enthalten in Bioprocess and biosystems engineering Springer-Verlag, 2001 33(2009), 4 vom: 02. Aug., Seite 473-483 (DE-627)333469763 (DE-600)2056063-1 (DE-576)094533709 1615-7591 nnns volume:33 year:2009 number:4 day:02 month:08 pages:473-483 https://doi.org/10.1007/s00449-009-0356-3 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-ARC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_70 GBV_ILN_2006 GBV_ILN_2018 GBV_ILN_4046 GBV_ILN_4277 GBV_ILN_4307 AR 33 2009 4 02 08 473-483 |
| language |
English |
| source |
Enthalten in Bioprocess and biosystems engineering 33(2009), 4 vom: 02. Aug., Seite 473-483 volume:33 year:2009 number:4 day:02 month:08 pages:473-483 |
| sourceStr |
Enthalten in Bioprocess and biosystems engineering 33(2009), 4 vom: 02. Aug., Seite 473-483 volume:33 year:2009 number:4 day:02 month:08 pages:473-483 |
| format_phy_str_mv |
Article |
| institution |
findex.gbv.de |
| topic_facet |
Fed-batch culture Multi-variables clustering Oxygen uptake rate (OUR) Porcine interferon-α expression |
| dewey-raw |
660 |
| isfreeaccess_bool |
false |
| container_title |
Bioprocess and biosystems engineering |
| authorswithroles_txt_mv |
Yu, Ruisong @@aut@@ Dong, Shijuan @@aut@@ Zhu, Yumin @@aut@@ Jin, Hu @@aut@@ Gao, Mingjie @@aut@@ Duan, Zuoying @@aut@@ Zheng, Zhiyong @@aut@@ Shi, Zhongping @@aut@@ Li, Zhen @@aut@@ |
| publishDateDaySort_date |
2009-08-02T00:00:00Z |
| hierarchy_top_id |
333469763 |
| dewey-sort |
3660 |
| id |
OLC2106617062 |
| language_de |
englisch |
| fullrecord |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000naa a22002652 4500</leader><controlfield tag="001">OLC2106617062</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230403073304.0</controlfield><controlfield tag="007">tu</controlfield><controlfield tag="008">230403s2009 xx ||||| 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/s00449-009-0356-3</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)OLC2106617062</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-He213)s00449-009-0356-3-p</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">660</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">570</subfield><subfield code="a">690</subfield><subfield code="a">540</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">12</subfield><subfield code="2">ssgn</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Yu, Ruisong</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Effective and stable porcine interferon-α production by Pichia pastoris fed-batch cultivation with multi-variables clustering and analysis</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2009</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">ohne Hilfsmittel zu benutzen</subfield><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Band</subfield><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">© Springer-Verlag 2009</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract Efficient porcine interferon-α (pIFN-α) expression in high density recombinant Pichia pastoris cultivation was achieved in a 5 l bench-scaled bioreactor. The results indicated that a high and stable oxygen uptake rate (OUR) during induction phase was closely related with pIFN-α production efficiency. The multi-variables clustering and analysis results showed that the achievement of a high and stable OUR relied on a higher glycerol consumption rate during fed-batch culture phase and a moderate methanol level (around 10 g/l) during induction phase. In the high and stable OUR environments (200–300 mmol/l/h), the highest pIFN-α antiviral activity could reach a level of 6.7 × $ 10^{6} $ IU/ml, which was more than 10–300-folds higher than those obtained at lower OUR (80–200 mmol/l/h) using the same bioreactor and those obtained in shaking flasks. Clustering and analysis of the specific growth and glycerol consumption rates data during culture phase could detect the ill fermentation state at early stage, potentially provided a simple and effective fault alarming/diagnosis method for the achievement of stable pIFN-α production.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Fed-batch culture</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Multi-variables clustering</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Oxygen uptake rate (OUR)</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Porcine interferon-α expression</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Dong, Shijuan</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Zhu, Yumin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Jin, Hu</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Gao, Mingjie</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Duan, Zuoying</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Zheng, Zhiyong</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Shi, Zhongping</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Li, Zhen</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Bioprocess and biosystems engineering</subfield><subfield code="d">Springer-Verlag, 2001</subfield><subfield code="g">33(2009), 4 vom: 02. Aug., Seite 473-483</subfield><subfield code="w">(DE-627)333469763</subfield><subfield code="w">(DE-600)2056063-1</subfield><subfield code="w">(DE-576)094533709</subfield><subfield code="x">1615-7591</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:33</subfield><subfield code="g">year:2009</subfield><subfield code="g">number:4</subfield><subfield code="g">day:02</subfield><subfield code="g">month:08</subfield><subfield code="g">pages:473-483</subfield></datafield><datafield tag="856" ind1="4" ind2="1"><subfield code="u">https://doi.org/10.1007/s00449-009-0356-3</subfield><subfield code="z">lizenzpflichtig</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_OLC</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-UMW</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-ARC</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-TEC</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-CHE</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_23</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_24</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_31</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_32</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_70</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2006</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2018</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4046</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4277</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4307</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">33</subfield><subfield code="j">2009</subfield><subfield code="e">4</subfield><subfield code="b">02</subfield><subfield code="c">08</subfield><subfield code="h">473-483</subfield></datafield></record></collection>
|
| author |
Yu, Ruisong |
| spellingShingle |
Yu, Ruisong ddc 660 ddc 570 ssgn 12 misc Fed-batch culture misc Multi-variables clustering misc Oxygen uptake rate (OUR) misc Porcine interferon-α expression Effective and stable porcine interferon-α production by Pichia pastoris fed-batch cultivation with multi-variables clustering and analysis |
| authorStr |
Yu, Ruisong |
| ppnlink_with_tag_str_mv |
@@773@@(DE-627)333469763 |
| format |
Article |
| dewey-ones |
660 - Chemical engineering 570 - Life sciences; biology 690 - Buildings 540 - Chemistry & allied sciences |
| delete_txt_mv |
keep |
| author_role |
aut aut aut aut aut aut aut aut aut |
| collection |
OLC |
| remote_str |
false |
| illustrated |
Not Illustrated |
| issn |
1615-7591 |
| topic_title |
660 VZ 570 690 540 VZ 12 ssgn Effective and stable porcine interferon-α production by Pichia pastoris fed-batch cultivation with multi-variables clustering and analysis Fed-batch culture Multi-variables clustering Oxygen uptake rate (OUR) Porcine interferon-α expression |
| topic |
ddc 660 ddc 570 ssgn 12 misc Fed-batch culture misc Multi-variables clustering misc Oxygen uptake rate (OUR) misc Porcine interferon-α expression |
| topic_unstemmed |
ddc 660 ddc 570 ssgn 12 misc Fed-batch culture misc Multi-variables clustering misc Oxygen uptake rate (OUR) misc Porcine interferon-α expression |
| topic_browse |
ddc 660 ddc 570 ssgn 12 misc Fed-batch culture misc Multi-variables clustering misc Oxygen uptake rate (OUR) misc Porcine interferon-α expression |
| format_facet |
Aufsätze Gedruckte Aufsätze |
| format_main_str_mv |
Text Zeitschrift/Artikel |
| carriertype_str_mv |
nc |
| hierarchy_parent_title |
Bioprocess and biosystems engineering |
| hierarchy_parent_id |
333469763 |
| dewey-tens |
660 - Chemical engineering 570 - Life sciences; biology 690 - Building & construction 540 - Chemistry |
| hierarchy_top_title |
Bioprocess and biosystems engineering |
| isfreeaccess_txt |
false |
| familylinks_str_mv |
(DE-627)333469763 (DE-600)2056063-1 (DE-576)094533709 |
| title |
Effective and stable porcine interferon-α production by Pichia pastoris fed-batch cultivation with multi-variables clustering and analysis |
| ctrlnum |
(DE-627)OLC2106617062 (DE-He213)s00449-009-0356-3-p |
| title_full |
Effective and stable porcine interferon-α production by Pichia pastoris fed-batch cultivation with multi-variables clustering and analysis |
| author_sort |
Yu, Ruisong |
| journal |
Bioprocess and biosystems engineering |
| journalStr |
Bioprocess and biosystems engineering |
| lang_code |
eng |
| isOA_bool |
false |
| dewey-hundreds |
600 - Technology 500 - Science |
| recordtype |
marc |
| publishDateSort |
2009 |
| contenttype_str_mv |
txt |
| container_start_page |
473 |
| author_browse |
Yu, Ruisong Dong, Shijuan Zhu, Yumin Jin, Hu Gao, Mingjie Duan, Zuoying Zheng, Zhiyong Shi, Zhongping Li, Zhen |
| container_volume |
33 |
| class |
660 VZ 570 690 540 VZ 12 ssgn |
| format_se |
Aufsätze |
| author-letter |
Yu, Ruisong |
| doi_str_mv |
10.1007/s00449-009-0356-3 |
| dewey-full |
660 570 690 540 |
| title_sort |
effective and stable porcine interferon-α production by pichia pastoris fed-batch cultivation with multi-variables clustering and analysis |
| title_auth |
Effective and stable porcine interferon-α production by Pichia pastoris fed-batch cultivation with multi-variables clustering and analysis |
| abstract |
Abstract Efficient porcine interferon-α (pIFN-α) expression in high density recombinant Pichia pastoris cultivation was achieved in a 5 l bench-scaled bioreactor. The results indicated that a high and stable oxygen uptake rate (OUR) during induction phase was closely related with pIFN-α production efficiency. The multi-variables clustering and analysis results showed that the achievement of a high and stable OUR relied on a higher glycerol consumption rate during fed-batch culture phase and a moderate methanol level (around 10 g/l) during induction phase. In the high and stable OUR environments (200–300 mmol/l/h), the highest pIFN-α antiviral activity could reach a level of 6.7 × $ 10^{6} $ IU/ml, which was more than 10–300-folds higher than those obtained at lower OUR (80–200 mmol/l/h) using the same bioreactor and those obtained in shaking flasks. Clustering and analysis of the specific growth and glycerol consumption rates data during culture phase could detect the ill fermentation state at early stage, potentially provided a simple and effective fault alarming/diagnosis method for the achievement of stable pIFN-α production. © Springer-Verlag 2009 |
| abstractGer |
Abstract Efficient porcine interferon-α (pIFN-α) expression in high density recombinant Pichia pastoris cultivation was achieved in a 5 l bench-scaled bioreactor. The results indicated that a high and stable oxygen uptake rate (OUR) during induction phase was closely related with pIFN-α production efficiency. The multi-variables clustering and analysis results showed that the achievement of a high and stable OUR relied on a higher glycerol consumption rate during fed-batch culture phase and a moderate methanol level (around 10 g/l) during induction phase. In the high and stable OUR environments (200–300 mmol/l/h), the highest pIFN-α antiviral activity could reach a level of 6.7 × $ 10^{6} $ IU/ml, which was more than 10–300-folds higher than those obtained at lower OUR (80–200 mmol/l/h) using the same bioreactor and those obtained in shaking flasks. Clustering and analysis of the specific growth and glycerol consumption rates data during culture phase could detect the ill fermentation state at early stage, potentially provided a simple and effective fault alarming/diagnosis method for the achievement of stable pIFN-α production. © Springer-Verlag 2009 |
| abstract_unstemmed |
Abstract Efficient porcine interferon-α (pIFN-α) expression in high density recombinant Pichia pastoris cultivation was achieved in a 5 l bench-scaled bioreactor. The results indicated that a high and stable oxygen uptake rate (OUR) during induction phase was closely related with pIFN-α production efficiency. The multi-variables clustering and analysis results showed that the achievement of a high and stable OUR relied on a higher glycerol consumption rate during fed-batch culture phase and a moderate methanol level (around 10 g/l) during induction phase. In the high and stable OUR environments (200–300 mmol/l/h), the highest pIFN-α antiviral activity could reach a level of 6.7 × $ 10^{6} $ IU/ml, which was more than 10–300-folds higher than those obtained at lower OUR (80–200 mmol/l/h) using the same bioreactor and those obtained in shaking flasks. Clustering and analysis of the specific growth and glycerol consumption rates data during culture phase could detect the ill fermentation state at early stage, potentially provided a simple and effective fault alarming/diagnosis method for the achievement of stable pIFN-α production. © Springer-Verlag 2009 |
| collection_details |
GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-ARC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_70 GBV_ILN_2006 GBV_ILN_2018 GBV_ILN_4046 GBV_ILN_4277 GBV_ILN_4307 |
| container_issue |
4 |
| title_short |
Effective and stable porcine interferon-α production by Pichia pastoris fed-batch cultivation with multi-variables clustering and analysis |
| url |
https://doi.org/10.1007/s00449-009-0356-3 |
| remote_bool |
false |
| author2 |
Dong, Shijuan Zhu, Yumin Jin, Hu Gao, Mingjie Duan, Zuoying Zheng, Zhiyong Shi, Zhongping Li, Zhen |
| author2Str |
Dong, Shijuan Zhu, Yumin Jin, Hu Gao, Mingjie Duan, Zuoying Zheng, Zhiyong Shi, Zhongping Li, Zhen |
| ppnlink |
333469763 |
| mediatype_str_mv |
n |
| isOA_txt |
false |
| hochschulschrift_bool |
false |
| doi_str |
10.1007/s00449-009-0356-3 |
| up_date |
2024-07-04T07:06:31.835Z |
| _version_ |
1803631242069934080 |
| fullrecord_marcxml |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000naa a22002652 4500</leader><controlfield tag="001">OLC2106617062</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230403073304.0</controlfield><controlfield tag="007">tu</controlfield><controlfield tag="008">230403s2009 xx ||||| 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/s00449-009-0356-3</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)OLC2106617062</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-He213)s00449-009-0356-3-p</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">660</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">570</subfield><subfield code="a">690</subfield><subfield code="a">540</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">12</subfield><subfield code="2">ssgn</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Yu, Ruisong</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Effective and stable porcine interferon-α production by Pichia pastoris fed-batch cultivation with multi-variables clustering and analysis</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2009</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">ohne Hilfsmittel zu benutzen</subfield><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Band</subfield><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">© Springer-Verlag 2009</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract Efficient porcine interferon-α (pIFN-α) expression in high density recombinant Pichia pastoris cultivation was achieved in a 5 l bench-scaled bioreactor. The results indicated that a high and stable oxygen uptake rate (OUR) during induction phase was closely related with pIFN-α production efficiency. The multi-variables clustering and analysis results showed that the achievement of a high and stable OUR relied on a higher glycerol consumption rate during fed-batch culture phase and a moderate methanol level (around 10 g/l) during induction phase. In the high and stable OUR environments (200–300 mmol/l/h), the highest pIFN-α antiviral activity could reach a level of 6.7 × $ 10^{6} $ IU/ml, which was more than 10–300-folds higher than those obtained at lower OUR (80–200 mmol/l/h) using the same bioreactor and those obtained in shaking flasks. Clustering and analysis of the specific growth and glycerol consumption rates data during culture phase could detect the ill fermentation state at early stage, potentially provided a simple and effective fault alarming/diagnosis method for the achievement of stable pIFN-α production.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Fed-batch culture</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Multi-variables clustering</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Oxygen uptake rate (OUR)</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Porcine interferon-α expression</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Dong, Shijuan</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Zhu, Yumin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Jin, Hu</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Gao, Mingjie</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Duan, Zuoying</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Zheng, Zhiyong</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Shi, Zhongping</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Li, Zhen</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Bioprocess and biosystems engineering</subfield><subfield code="d">Springer-Verlag, 2001</subfield><subfield code="g">33(2009), 4 vom: 02. Aug., Seite 473-483</subfield><subfield code="w">(DE-627)333469763</subfield><subfield code="w">(DE-600)2056063-1</subfield><subfield code="w">(DE-576)094533709</subfield><subfield code="x">1615-7591</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:33</subfield><subfield code="g">year:2009</subfield><subfield code="g">number:4</subfield><subfield code="g">day:02</subfield><subfield code="g">month:08</subfield><subfield code="g">pages:473-483</subfield></datafield><datafield tag="856" ind1="4" ind2="1"><subfield code="u">https://doi.org/10.1007/s00449-009-0356-3</subfield><subfield code="z">lizenzpflichtig</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_OLC</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-UMW</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-ARC</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-TEC</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-CHE</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_23</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_24</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_31</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_32</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_70</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2006</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2018</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4046</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4277</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4307</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">33</subfield><subfield code="j">2009</subfield><subfield code="e">4</subfield><subfield code="b">02</subfield><subfield code="c">08</subfield><subfield code="h">473-483</subfield></datafield></record></collection>
|
| score |
7.3990374 |