Transcriptomic study for screening genes involved in the oxidative bioconversions of Streptomyces avermitilis
Abstract Streptomyces avermitilis is a well known organism producing avermectin antibiotics, and has been utilized as an industrial host for oxidation bioconversion processes. Recently, gene screening strategies related to bioconversions have received much focus, as attempts are made to optimize oxi...
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Gespeichert in:
| Autor*in: |
Kim, Hyo-Jeong [verfasserIn] |
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| Format: |
Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2013 |
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| Schlagwörter: |
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| Anmerkung: |
© Springer-Verlag Berlin Heidelberg 2013 |
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| Übergeordnetes Werk: |
Enthalten in: Bioprocess and biosystems engineering - Springer Berlin Heidelberg, 2001, 36(2013), 11 vom: 09. März, Seite 1621-1630 |
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| Übergeordnetes Werk: |
volume:36 ; year:2013 ; number:11 ; day:09 ; month:03 ; pages:1621-1630 |
| Links: |
|---|
| DOI / URN: |
10.1007/s00449-013-0935-1 |
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| Katalog-ID: |
OLC2106625359 |
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| 520 | |a Abstract Streptomyces avermitilis is a well known organism producing avermectin antibiotics, and has been utilized as an industrial host for oxidation bioconversion processes. Recently, gene screening strategies related to bioconversions have received much focus, as attempts are made to optimize oxidation and biodegradation pathways to maximize yield and productivity. Here, we have demonstrated the oxidative metabolisms of three molecules, daidzein, p-coumaric acid and mevastatin, where S. avermitilis converted each substrate to 3′,4′,7-trihydroxyisoflavone, caffeic acid and hydroxyl-mevastatin to yield 9.3, 32.5 and 15.0 %, respectively. Microarray technology was exploited to investigate genome-wide analysis of gene expression changes, which were induced upon the addition of each substrate. Cytochrome P450 hydroxylases (pteC, cyp28 and olmB), diooxygenases (xylE, cdo1 and putatives) and LuxAB-like oxygenase were identified. One of them, cyp28, was indeed a gene encoding P450 hydroxylase responsible for the oxidative reaction of daidzein. Furthermore, possible electron transfer chain (fdrC → pteE → pteC) supporting cytochrome P450 dependent hydroxylation of daidzein has been suggested based on the interpretation of expression profiles. The result provided a potential application of transcriptomic study on uncovering enzymes involved in oxidative bioconversions of S. avermitilis. | ||
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| 700 | 1 | |a Kim, Byung-Gee |4 aut | |
| 700 | 1 | |a Oh, Min-Kyu |4 aut | |
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10.1007/s00449-013-0935-1 doi (DE-627)OLC2106625359 (DE-He213)s00449-013-0935-1-p DE-627 ger DE-627 rakwb eng 660 VZ 570 690 540 VZ 12 ssgn Kim, Hyo-Jeong verfasserin aut Transcriptomic study for screening genes involved in the oxidative bioconversions of Streptomyces avermitilis 2013 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag Berlin Heidelberg 2013 Abstract Streptomyces avermitilis is a well known organism producing avermectin antibiotics, and has been utilized as an industrial host for oxidation bioconversion processes. Recently, gene screening strategies related to bioconversions have received much focus, as attempts are made to optimize oxidation and biodegradation pathways to maximize yield and productivity. Here, we have demonstrated the oxidative metabolisms of three molecules, daidzein, p-coumaric acid and mevastatin, where S. avermitilis converted each substrate to 3′,4′,7-trihydroxyisoflavone, caffeic acid and hydroxyl-mevastatin to yield 9.3, 32.5 and 15.0 %, respectively. Microarray technology was exploited to investigate genome-wide analysis of gene expression changes, which were induced upon the addition of each substrate. Cytochrome P450 hydroxylases (pteC, cyp28 and olmB), diooxygenases (xylE, cdo1 and putatives) and LuxAB-like oxygenase were identified. One of them, cyp28, was indeed a gene encoding P450 hydroxylase responsible for the oxidative reaction of daidzein. Furthermore, possible electron transfer chain (fdrC → pteE → pteC) supporting cytochrome P450 dependent hydroxylation of daidzein has been suggested based on the interpretation of expression profiles. The result provided a potential application of transcriptomic study on uncovering enzymes involved in oxidative bioconversions of S. avermitilis. Transcriptomics Daidzein Biotransformation Choi, Kwon-Young aut Jung, Da-Hye aut Jung, Joon-Young aut Jung, EunOk aut Yang, Yung-Hun aut Kim, Byung-Gee aut Oh, Min-Kyu aut Enthalten in Bioprocess and biosystems engineering Springer Berlin Heidelberg, 2001 36(2013), 11 vom: 09. März, Seite 1621-1630 (DE-627)333469763 (DE-600)2056063-1 (DE-576)094533709 1615-7591 nnns volume:36 year:2013 number:11 day:09 month:03 pages:1621-1630 https://doi.org/10.1007/s00449-013-0935-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-ARC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_70 GBV_ILN_2018 GBV_ILN_4046 GBV_ILN_4277 AR 36 2013 11 09 03 1621-1630 |
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10.1007/s00449-013-0935-1 doi (DE-627)OLC2106625359 (DE-He213)s00449-013-0935-1-p DE-627 ger DE-627 rakwb eng 660 VZ 570 690 540 VZ 12 ssgn Kim, Hyo-Jeong verfasserin aut Transcriptomic study for screening genes involved in the oxidative bioconversions of Streptomyces avermitilis 2013 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag Berlin Heidelberg 2013 Abstract Streptomyces avermitilis is a well known organism producing avermectin antibiotics, and has been utilized as an industrial host for oxidation bioconversion processes. Recently, gene screening strategies related to bioconversions have received much focus, as attempts are made to optimize oxidation and biodegradation pathways to maximize yield and productivity. Here, we have demonstrated the oxidative metabolisms of three molecules, daidzein, p-coumaric acid and mevastatin, where S. avermitilis converted each substrate to 3′,4′,7-trihydroxyisoflavone, caffeic acid and hydroxyl-mevastatin to yield 9.3, 32.5 and 15.0 %, respectively. Microarray technology was exploited to investigate genome-wide analysis of gene expression changes, which were induced upon the addition of each substrate. Cytochrome P450 hydroxylases (pteC, cyp28 and olmB), diooxygenases (xylE, cdo1 and putatives) and LuxAB-like oxygenase were identified. One of them, cyp28, was indeed a gene encoding P450 hydroxylase responsible for the oxidative reaction of daidzein. Furthermore, possible electron transfer chain (fdrC → pteE → pteC) supporting cytochrome P450 dependent hydroxylation of daidzein has been suggested based on the interpretation of expression profiles. The result provided a potential application of transcriptomic study on uncovering enzymes involved in oxidative bioconversions of S. avermitilis. Transcriptomics Daidzein Biotransformation Choi, Kwon-Young aut Jung, Da-Hye aut Jung, Joon-Young aut Jung, EunOk aut Yang, Yung-Hun aut Kim, Byung-Gee aut Oh, Min-Kyu aut Enthalten in Bioprocess and biosystems engineering Springer Berlin Heidelberg, 2001 36(2013), 11 vom: 09. März, Seite 1621-1630 (DE-627)333469763 (DE-600)2056063-1 (DE-576)094533709 1615-7591 nnns volume:36 year:2013 number:11 day:09 month:03 pages:1621-1630 https://doi.org/10.1007/s00449-013-0935-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-ARC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_70 GBV_ILN_2018 GBV_ILN_4046 GBV_ILN_4277 AR 36 2013 11 09 03 1621-1630 |
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10.1007/s00449-013-0935-1 doi (DE-627)OLC2106625359 (DE-He213)s00449-013-0935-1-p DE-627 ger DE-627 rakwb eng 660 VZ 570 690 540 VZ 12 ssgn Kim, Hyo-Jeong verfasserin aut Transcriptomic study for screening genes involved in the oxidative bioconversions of Streptomyces avermitilis 2013 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag Berlin Heidelberg 2013 Abstract Streptomyces avermitilis is a well known organism producing avermectin antibiotics, and has been utilized as an industrial host for oxidation bioconversion processes. Recently, gene screening strategies related to bioconversions have received much focus, as attempts are made to optimize oxidation and biodegradation pathways to maximize yield and productivity. Here, we have demonstrated the oxidative metabolisms of three molecules, daidzein, p-coumaric acid and mevastatin, where S. avermitilis converted each substrate to 3′,4′,7-trihydroxyisoflavone, caffeic acid and hydroxyl-mevastatin to yield 9.3, 32.5 and 15.0 %, respectively. Microarray technology was exploited to investigate genome-wide analysis of gene expression changes, which were induced upon the addition of each substrate. Cytochrome P450 hydroxylases (pteC, cyp28 and olmB), diooxygenases (xylE, cdo1 and putatives) and LuxAB-like oxygenase were identified. One of them, cyp28, was indeed a gene encoding P450 hydroxylase responsible for the oxidative reaction of daidzein. Furthermore, possible electron transfer chain (fdrC → pteE → pteC) supporting cytochrome P450 dependent hydroxylation of daidzein has been suggested based on the interpretation of expression profiles. The result provided a potential application of transcriptomic study on uncovering enzymes involved in oxidative bioconversions of S. avermitilis. Transcriptomics Daidzein Biotransformation Choi, Kwon-Young aut Jung, Da-Hye aut Jung, Joon-Young aut Jung, EunOk aut Yang, Yung-Hun aut Kim, Byung-Gee aut Oh, Min-Kyu aut Enthalten in Bioprocess and biosystems engineering Springer Berlin Heidelberg, 2001 36(2013), 11 vom: 09. März, Seite 1621-1630 (DE-627)333469763 (DE-600)2056063-1 (DE-576)094533709 1615-7591 nnns volume:36 year:2013 number:11 day:09 month:03 pages:1621-1630 https://doi.org/10.1007/s00449-013-0935-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-ARC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_70 GBV_ILN_2018 GBV_ILN_4046 GBV_ILN_4277 AR 36 2013 11 09 03 1621-1630 |
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10.1007/s00449-013-0935-1 doi (DE-627)OLC2106625359 (DE-He213)s00449-013-0935-1-p DE-627 ger DE-627 rakwb eng 660 VZ 570 690 540 VZ 12 ssgn Kim, Hyo-Jeong verfasserin aut Transcriptomic study for screening genes involved in the oxidative bioconversions of Streptomyces avermitilis 2013 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag Berlin Heidelberg 2013 Abstract Streptomyces avermitilis is a well known organism producing avermectin antibiotics, and has been utilized as an industrial host for oxidation bioconversion processes. Recently, gene screening strategies related to bioconversions have received much focus, as attempts are made to optimize oxidation and biodegradation pathways to maximize yield and productivity. Here, we have demonstrated the oxidative metabolisms of three molecules, daidzein, p-coumaric acid and mevastatin, where S. avermitilis converted each substrate to 3′,4′,7-trihydroxyisoflavone, caffeic acid and hydroxyl-mevastatin to yield 9.3, 32.5 and 15.0 %, respectively. Microarray technology was exploited to investigate genome-wide analysis of gene expression changes, which were induced upon the addition of each substrate. Cytochrome P450 hydroxylases (pteC, cyp28 and olmB), diooxygenases (xylE, cdo1 and putatives) and LuxAB-like oxygenase were identified. One of them, cyp28, was indeed a gene encoding P450 hydroxylase responsible for the oxidative reaction of daidzein. Furthermore, possible electron transfer chain (fdrC → pteE → pteC) supporting cytochrome P450 dependent hydroxylation of daidzein has been suggested based on the interpretation of expression profiles. The result provided a potential application of transcriptomic study on uncovering enzymes involved in oxidative bioconversions of S. avermitilis. Transcriptomics Daidzein Biotransformation Choi, Kwon-Young aut Jung, Da-Hye aut Jung, Joon-Young aut Jung, EunOk aut Yang, Yung-Hun aut Kim, Byung-Gee aut Oh, Min-Kyu aut Enthalten in Bioprocess and biosystems engineering Springer Berlin Heidelberg, 2001 36(2013), 11 vom: 09. März, Seite 1621-1630 (DE-627)333469763 (DE-600)2056063-1 (DE-576)094533709 1615-7591 nnns volume:36 year:2013 number:11 day:09 month:03 pages:1621-1630 https://doi.org/10.1007/s00449-013-0935-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-ARC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_70 GBV_ILN_2018 GBV_ILN_4046 GBV_ILN_4277 AR 36 2013 11 09 03 1621-1630 |
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10.1007/s00449-013-0935-1 doi (DE-627)OLC2106625359 (DE-He213)s00449-013-0935-1-p DE-627 ger DE-627 rakwb eng 660 VZ 570 690 540 VZ 12 ssgn Kim, Hyo-Jeong verfasserin aut Transcriptomic study for screening genes involved in the oxidative bioconversions of Streptomyces avermitilis 2013 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag Berlin Heidelberg 2013 Abstract Streptomyces avermitilis is a well known organism producing avermectin antibiotics, and has been utilized as an industrial host for oxidation bioconversion processes. Recently, gene screening strategies related to bioconversions have received much focus, as attempts are made to optimize oxidation and biodegradation pathways to maximize yield and productivity. Here, we have demonstrated the oxidative metabolisms of three molecules, daidzein, p-coumaric acid and mevastatin, where S. avermitilis converted each substrate to 3′,4′,7-trihydroxyisoflavone, caffeic acid and hydroxyl-mevastatin to yield 9.3, 32.5 and 15.0 %, respectively. Microarray technology was exploited to investigate genome-wide analysis of gene expression changes, which were induced upon the addition of each substrate. Cytochrome P450 hydroxylases (pteC, cyp28 and olmB), diooxygenases (xylE, cdo1 and putatives) and LuxAB-like oxygenase were identified. One of them, cyp28, was indeed a gene encoding P450 hydroxylase responsible for the oxidative reaction of daidzein. Furthermore, possible electron transfer chain (fdrC → pteE → pteC) supporting cytochrome P450 dependent hydroxylation of daidzein has been suggested based on the interpretation of expression profiles. The result provided a potential application of transcriptomic study on uncovering enzymes involved in oxidative bioconversions of S. avermitilis. Transcriptomics Daidzein Biotransformation Choi, Kwon-Young aut Jung, Da-Hye aut Jung, Joon-Young aut Jung, EunOk aut Yang, Yung-Hun aut Kim, Byung-Gee aut Oh, Min-Kyu aut Enthalten in Bioprocess and biosystems engineering Springer Berlin Heidelberg, 2001 36(2013), 11 vom: 09. März, Seite 1621-1630 (DE-627)333469763 (DE-600)2056063-1 (DE-576)094533709 1615-7591 nnns volume:36 year:2013 number:11 day:09 month:03 pages:1621-1630 https://doi.org/10.1007/s00449-013-0935-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-ARC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_70 GBV_ILN_2018 GBV_ILN_4046 GBV_ILN_4277 AR 36 2013 11 09 03 1621-1630 |
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transcriptomic study for screening genes involved in the oxidative bioconversions of streptomyces avermitilis |
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Transcriptomic study for screening genes involved in the oxidative bioconversions of Streptomyces avermitilis |
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Abstract Streptomyces avermitilis is a well known organism producing avermectin antibiotics, and has been utilized as an industrial host for oxidation bioconversion processes. Recently, gene screening strategies related to bioconversions have received much focus, as attempts are made to optimize oxidation and biodegradation pathways to maximize yield and productivity. Here, we have demonstrated the oxidative metabolisms of three molecules, daidzein, p-coumaric acid and mevastatin, where S. avermitilis converted each substrate to 3′,4′,7-trihydroxyisoflavone, caffeic acid and hydroxyl-mevastatin to yield 9.3, 32.5 and 15.0 %, respectively. Microarray technology was exploited to investigate genome-wide analysis of gene expression changes, which were induced upon the addition of each substrate. Cytochrome P450 hydroxylases (pteC, cyp28 and olmB), diooxygenases (xylE, cdo1 and putatives) and LuxAB-like oxygenase were identified. One of them, cyp28, was indeed a gene encoding P450 hydroxylase responsible for the oxidative reaction of daidzein. Furthermore, possible electron transfer chain (fdrC → pteE → pteC) supporting cytochrome P450 dependent hydroxylation of daidzein has been suggested based on the interpretation of expression profiles. The result provided a potential application of transcriptomic study on uncovering enzymes involved in oxidative bioconversions of S. avermitilis. © Springer-Verlag Berlin Heidelberg 2013 |
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Abstract Streptomyces avermitilis is a well known organism producing avermectin antibiotics, and has been utilized as an industrial host for oxidation bioconversion processes. Recently, gene screening strategies related to bioconversions have received much focus, as attempts are made to optimize oxidation and biodegradation pathways to maximize yield and productivity. Here, we have demonstrated the oxidative metabolisms of three molecules, daidzein, p-coumaric acid and mevastatin, where S. avermitilis converted each substrate to 3′,4′,7-trihydroxyisoflavone, caffeic acid and hydroxyl-mevastatin to yield 9.3, 32.5 and 15.0 %, respectively. Microarray technology was exploited to investigate genome-wide analysis of gene expression changes, which were induced upon the addition of each substrate. Cytochrome P450 hydroxylases (pteC, cyp28 and olmB), diooxygenases (xylE, cdo1 and putatives) and LuxAB-like oxygenase were identified. One of them, cyp28, was indeed a gene encoding P450 hydroxylase responsible for the oxidative reaction of daidzein. Furthermore, possible electron transfer chain (fdrC → pteE → pteC) supporting cytochrome P450 dependent hydroxylation of daidzein has been suggested based on the interpretation of expression profiles. The result provided a potential application of transcriptomic study on uncovering enzymes involved in oxidative bioconversions of S. avermitilis. © Springer-Verlag Berlin Heidelberg 2013 |
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Abstract Streptomyces avermitilis is a well known organism producing avermectin antibiotics, and has been utilized as an industrial host for oxidation bioconversion processes. Recently, gene screening strategies related to bioconversions have received much focus, as attempts are made to optimize oxidation and biodegradation pathways to maximize yield and productivity. Here, we have demonstrated the oxidative metabolisms of three molecules, daidzein, p-coumaric acid and mevastatin, where S. avermitilis converted each substrate to 3′,4′,7-trihydroxyisoflavone, caffeic acid and hydroxyl-mevastatin to yield 9.3, 32.5 and 15.0 %, respectively. Microarray technology was exploited to investigate genome-wide analysis of gene expression changes, which were induced upon the addition of each substrate. Cytochrome P450 hydroxylases (pteC, cyp28 and olmB), diooxygenases (xylE, cdo1 and putatives) and LuxAB-like oxygenase were identified. One of them, cyp28, was indeed a gene encoding P450 hydroxylase responsible for the oxidative reaction of daidzein. Furthermore, possible electron transfer chain (fdrC → pteE → pteC) supporting cytochrome P450 dependent hydroxylation of daidzein has been suggested based on the interpretation of expression profiles. The result provided a potential application of transcriptomic study on uncovering enzymes involved in oxidative bioconversions of S. avermitilis. © Springer-Verlag Berlin Heidelberg 2013 |
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