Liquid perfluorochemical-supported hybrid cell culture system for proliferation of chondrocytes on fibrous polylactide scaffolds
Abstract CP5 bovine chondrocytes were cultured on biodegradable electrospun fibrous polylactide (PLA) scaffolds placed on a flexible interface formed between two immiscible liquid phases: (1) hydrophobic perfluorochemical (PFC) and (2) aqueous culture medium, as a new way of cartilage implant develo...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Pilarek, Maciej [verfasserIn] |
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| Format: |
Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2014 |
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| Schlagwörter: |
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| Anmerkung: |
© The Author(s) 2014 |
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| Übergeordnetes Werk: |
Enthalten in: Bioprocess and biosystems engineering - Springer Berlin Heidelberg, 2001, 37(2014), 9 vom: 16. Feb., Seite 1707-1715 |
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| Übergeordnetes Werk: |
volume:37 ; year:2014 ; number:9 ; day:16 ; month:02 ; pages:1707-1715 |
| Links: |
|---|
| DOI / URN: |
10.1007/s00449-014-1143-3 |
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| Katalog-ID: |
OLC210663367X |
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| 520 | |a Abstract CP5 bovine chondrocytes were cultured on biodegradable electrospun fibrous polylactide (PLA) scaffolds placed on a flexible interface formed between two immiscible liquid phases: (1) hydrophobic perfluorochemical (PFC) and (2) aqueous culture medium, as a new way of cartilage implant development. Robust and intensive growth of CP5 cells was achieved in our hybrid liquid–solid–liquid culture system consisting of the fibrous PLA scaffolds in contrast to limited growth of the CP5 cells in traditional culture system with PLA scaffold placed on solid surface. The multicellular aggregates of CP5 cells covered the surface of PLA scaffolds and the chondrocytes migrated through and overgrew internal fibers of the scaffolds. Our hybrid culture system simultaneously allows the adhesion of adherent CP5 cells to fibers of PLA scaffolds as well as, due to use of phase of PFC, enhances the mass transfer in the case of supplying/removing of respiratory gases, i.e., $ O_{2} $ and $ CO_{2} $. Our flexible (independent of vessel shape) system is simple, ready-to-use and may utilize a variety of polymer-based scaffolds traditionally proposed for implant development. | ||
| 650 | 4 | |a Liquid/liquid culture system | |
| 650 | 4 | |a Perfluorochemical (fluorocarbon) | |
| 650 | 4 | |a Polylactide (PLA) | |
| 650 | 4 | |a Fibrous scaffold | |
| 650 | 4 | |a Electrospinning | |
| 650 | 4 | |a Chondrocytes | |
| 700 | 1 | |a Grabowska, Iwona |4 aut | |
| 700 | 1 | |a Senderek, Ilona |4 aut | |
| 700 | 1 | |a Wojasiński, Michał |4 aut | |
| 700 | 1 | |a Janicka, Justyna |4 aut | |
| 700 | 1 | |a Janczyk-Ilach, Katarzyna |4 aut | |
| 700 | 1 | |a Ciach, Tomasz |4 aut | |
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10.1007/s00449-014-1143-3 doi (DE-627)OLC210663367X (DE-He213)s00449-014-1143-3-p DE-627 ger DE-627 rakwb eng 660 VZ 570 690 540 VZ 12 ssgn Pilarek, Maciej verfasserin aut Liquid perfluorochemical-supported hybrid cell culture system for proliferation of chondrocytes on fibrous polylactide scaffolds 2014 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © The Author(s) 2014 Abstract CP5 bovine chondrocytes were cultured on biodegradable electrospun fibrous polylactide (PLA) scaffolds placed on a flexible interface formed between two immiscible liquid phases: (1) hydrophobic perfluorochemical (PFC) and (2) aqueous culture medium, as a new way of cartilage implant development. Robust and intensive growth of CP5 cells was achieved in our hybrid liquid–solid–liquid culture system consisting of the fibrous PLA scaffolds in contrast to limited growth of the CP5 cells in traditional culture system with PLA scaffold placed on solid surface. The multicellular aggregates of CP5 cells covered the surface of PLA scaffolds and the chondrocytes migrated through and overgrew internal fibers of the scaffolds. Our hybrid culture system simultaneously allows the adhesion of adherent CP5 cells to fibers of PLA scaffolds as well as, due to use of phase of PFC, enhances the mass transfer in the case of supplying/removing of respiratory gases, i.e., $ O_{2} $ and $ CO_{2} $. Our flexible (independent of vessel shape) system is simple, ready-to-use and may utilize a variety of polymer-based scaffolds traditionally proposed for implant development. Liquid/liquid culture system Perfluorochemical (fluorocarbon) Polylactide (PLA) Fibrous scaffold Electrospinning Chondrocytes Grabowska, Iwona aut Senderek, Ilona aut Wojasiński, Michał aut Janicka, Justyna aut Janczyk-Ilach, Katarzyna aut Ciach, Tomasz aut Enthalten in Bioprocess and biosystems engineering Springer Berlin Heidelberg, 2001 37(2014), 9 vom: 16. Feb., Seite 1707-1715 (DE-627)333469763 (DE-600)2056063-1 (DE-576)094533709 1615-7591 nnns volume:37 year:2014 number:9 day:16 month:02 pages:1707-1715 https://doi.org/10.1007/s00449-014-1143-3 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-ARC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_70 GBV_ILN_2018 GBV_ILN_4046 GBV_ILN_4277 AR 37 2014 9 16 02 1707-1715 |
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10.1007/s00449-014-1143-3 doi (DE-627)OLC210663367X (DE-He213)s00449-014-1143-3-p DE-627 ger DE-627 rakwb eng 660 VZ 570 690 540 VZ 12 ssgn Pilarek, Maciej verfasserin aut Liquid perfluorochemical-supported hybrid cell culture system for proliferation of chondrocytes on fibrous polylactide scaffolds 2014 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © The Author(s) 2014 Abstract CP5 bovine chondrocytes were cultured on biodegradable electrospun fibrous polylactide (PLA) scaffolds placed on a flexible interface formed between two immiscible liquid phases: (1) hydrophobic perfluorochemical (PFC) and (2) aqueous culture medium, as a new way of cartilage implant development. Robust and intensive growth of CP5 cells was achieved in our hybrid liquid–solid–liquid culture system consisting of the fibrous PLA scaffolds in contrast to limited growth of the CP5 cells in traditional culture system with PLA scaffold placed on solid surface. The multicellular aggregates of CP5 cells covered the surface of PLA scaffolds and the chondrocytes migrated through and overgrew internal fibers of the scaffolds. Our hybrid culture system simultaneously allows the adhesion of adherent CP5 cells to fibers of PLA scaffolds as well as, due to use of phase of PFC, enhances the mass transfer in the case of supplying/removing of respiratory gases, i.e., $ O_{2} $ and $ CO_{2} $. Our flexible (independent of vessel shape) system is simple, ready-to-use and may utilize a variety of polymer-based scaffolds traditionally proposed for implant development. Liquid/liquid culture system Perfluorochemical (fluorocarbon) Polylactide (PLA) Fibrous scaffold Electrospinning Chondrocytes Grabowska, Iwona aut Senderek, Ilona aut Wojasiński, Michał aut Janicka, Justyna aut Janczyk-Ilach, Katarzyna aut Ciach, Tomasz aut Enthalten in Bioprocess and biosystems engineering Springer Berlin Heidelberg, 2001 37(2014), 9 vom: 16. Feb., Seite 1707-1715 (DE-627)333469763 (DE-600)2056063-1 (DE-576)094533709 1615-7591 nnns volume:37 year:2014 number:9 day:16 month:02 pages:1707-1715 https://doi.org/10.1007/s00449-014-1143-3 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-ARC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_70 GBV_ILN_2018 GBV_ILN_4046 GBV_ILN_4277 AR 37 2014 9 16 02 1707-1715 |
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10.1007/s00449-014-1143-3 doi (DE-627)OLC210663367X (DE-He213)s00449-014-1143-3-p DE-627 ger DE-627 rakwb eng 660 VZ 570 690 540 VZ 12 ssgn Pilarek, Maciej verfasserin aut Liquid perfluorochemical-supported hybrid cell culture system for proliferation of chondrocytes on fibrous polylactide scaffolds 2014 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © The Author(s) 2014 Abstract CP5 bovine chondrocytes were cultured on biodegradable electrospun fibrous polylactide (PLA) scaffolds placed on a flexible interface formed between two immiscible liquid phases: (1) hydrophobic perfluorochemical (PFC) and (2) aqueous culture medium, as a new way of cartilage implant development. Robust and intensive growth of CP5 cells was achieved in our hybrid liquid–solid–liquid culture system consisting of the fibrous PLA scaffolds in contrast to limited growth of the CP5 cells in traditional culture system with PLA scaffold placed on solid surface. The multicellular aggregates of CP5 cells covered the surface of PLA scaffolds and the chondrocytes migrated through and overgrew internal fibers of the scaffolds. Our hybrid culture system simultaneously allows the adhesion of adherent CP5 cells to fibers of PLA scaffolds as well as, due to use of phase of PFC, enhances the mass transfer in the case of supplying/removing of respiratory gases, i.e., $ O_{2} $ and $ CO_{2} $. Our flexible (independent of vessel shape) system is simple, ready-to-use and may utilize a variety of polymer-based scaffolds traditionally proposed for implant development. Liquid/liquid culture system Perfluorochemical (fluorocarbon) Polylactide (PLA) Fibrous scaffold Electrospinning Chondrocytes Grabowska, Iwona aut Senderek, Ilona aut Wojasiński, Michał aut Janicka, Justyna aut Janczyk-Ilach, Katarzyna aut Ciach, Tomasz aut Enthalten in Bioprocess and biosystems engineering Springer Berlin Heidelberg, 2001 37(2014), 9 vom: 16. Feb., Seite 1707-1715 (DE-627)333469763 (DE-600)2056063-1 (DE-576)094533709 1615-7591 nnns volume:37 year:2014 number:9 day:16 month:02 pages:1707-1715 https://doi.org/10.1007/s00449-014-1143-3 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-ARC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_70 GBV_ILN_2018 GBV_ILN_4046 GBV_ILN_4277 AR 37 2014 9 16 02 1707-1715 |
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10.1007/s00449-014-1143-3 doi (DE-627)OLC210663367X (DE-He213)s00449-014-1143-3-p DE-627 ger DE-627 rakwb eng 660 VZ 570 690 540 VZ 12 ssgn Pilarek, Maciej verfasserin aut Liquid perfluorochemical-supported hybrid cell culture system for proliferation of chondrocytes on fibrous polylactide scaffolds 2014 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © The Author(s) 2014 Abstract CP5 bovine chondrocytes were cultured on biodegradable electrospun fibrous polylactide (PLA) scaffolds placed on a flexible interface formed between two immiscible liquid phases: (1) hydrophobic perfluorochemical (PFC) and (2) aqueous culture medium, as a new way of cartilage implant development. Robust and intensive growth of CP5 cells was achieved in our hybrid liquid–solid–liquid culture system consisting of the fibrous PLA scaffolds in contrast to limited growth of the CP5 cells in traditional culture system with PLA scaffold placed on solid surface. The multicellular aggregates of CP5 cells covered the surface of PLA scaffolds and the chondrocytes migrated through and overgrew internal fibers of the scaffolds. Our hybrid culture system simultaneously allows the adhesion of adherent CP5 cells to fibers of PLA scaffolds as well as, due to use of phase of PFC, enhances the mass transfer in the case of supplying/removing of respiratory gases, i.e., $ O_{2} $ and $ CO_{2} $. Our flexible (independent of vessel shape) system is simple, ready-to-use and may utilize a variety of polymer-based scaffolds traditionally proposed for implant development. Liquid/liquid culture system Perfluorochemical (fluorocarbon) Polylactide (PLA) Fibrous scaffold Electrospinning Chondrocytes Grabowska, Iwona aut Senderek, Ilona aut Wojasiński, Michał aut Janicka, Justyna aut Janczyk-Ilach, Katarzyna aut Ciach, Tomasz aut Enthalten in Bioprocess and biosystems engineering Springer Berlin Heidelberg, 2001 37(2014), 9 vom: 16. Feb., Seite 1707-1715 (DE-627)333469763 (DE-600)2056063-1 (DE-576)094533709 1615-7591 nnns volume:37 year:2014 number:9 day:16 month:02 pages:1707-1715 https://doi.org/10.1007/s00449-014-1143-3 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-ARC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_70 GBV_ILN_2018 GBV_ILN_4046 GBV_ILN_4277 AR 37 2014 9 16 02 1707-1715 |
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10.1007/s00449-014-1143-3 doi (DE-627)OLC210663367X (DE-He213)s00449-014-1143-3-p DE-627 ger DE-627 rakwb eng 660 VZ 570 690 540 VZ 12 ssgn Pilarek, Maciej verfasserin aut Liquid perfluorochemical-supported hybrid cell culture system for proliferation of chondrocytes on fibrous polylactide scaffolds 2014 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © The Author(s) 2014 Abstract CP5 bovine chondrocytes were cultured on biodegradable electrospun fibrous polylactide (PLA) scaffolds placed on a flexible interface formed between two immiscible liquid phases: (1) hydrophobic perfluorochemical (PFC) and (2) aqueous culture medium, as a new way of cartilage implant development. Robust and intensive growth of CP5 cells was achieved in our hybrid liquid–solid–liquid culture system consisting of the fibrous PLA scaffolds in contrast to limited growth of the CP5 cells in traditional culture system with PLA scaffold placed on solid surface. The multicellular aggregates of CP5 cells covered the surface of PLA scaffolds and the chondrocytes migrated through and overgrew internal fibers of the scaffolds. Our hybrid culture system simultaneously allows the adhesion of adherent CP5 cells to fibers of PLA scaffolds as well as, due to use of phase of PFC, enhances the mass transfer in the case of supplying/removing of respiratory gases, i.e., $ O_{2} $ and $ CO_{2} $. Our flexible (independent of vessel shape) system is simple, ready-to-use and may utilize a variety of polymer-based scaffolds traditionally proposed for implant development. Liquid/liquid culture system Perfluorochemical (fluorocarbon) Polylactide (PLA) Fibrous scaffold Electrospinning Chondrocytes Grabowska, Iwona aut Senderek, Ilona aut Wojasiński, Michał aut Janicka, Justyna aut Janczyk-Ilach, Katarzyna aut Ciach, Tomasz aut Enthalten in Bioprocess and biosystems engineering Springer Berlin Heidelberg, 2001 37(2014), 9 vom: 16. Feb., Seite 1707-1715 (DE-627)333469763 (DE-600)2056063-1 (DE-576)094533709 1615-7591 nnns volume:37 year:2014 number:9 day:16 month:02 pages:1707-1715 https://doi.org/10.1007/s00449-014-1143-3 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-ARC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_70 GBV_ILN_2018 GBV_ILN_4046 GBV_ILN_4277 AR 37 2014 9 16 02 1707-1715 |
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Enthalten in Bioprocess and biosystems engineering 37(2014), 9 vom: 16. Feb., Seite 1707-1715 volume:37 year:2014 number:9 day:16 month:02 pages:1707-1715 |
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liquid perfluorochemical-supported hybrid cell culture system for proliferation of chondrocytes on fibrous polylactide scaffolds |
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Liquid perfluorochemical-supported hybrid cell culture system for proliferation of chondrocytes on fibrous polylactide scaffolds |
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Abstract CP5 bovine chondrocytes were cultured on biodegradable electrospun fibrous polylactide (PLA) scaffolds placed on a flexible interface formed between two immiscible liquid phases: (1) hydrophobic perfluorochemical (PFC) and (2) aqueous culture medium, as a new way of cartilage implant development. Robust and intensive growth of CP5 cells was achieved in our hybrid liquid–solid–liquid culture system consisting of the fibrous PLA scaffolds in contrast to limited growth of the CP5 cells in traditional culture system with PLA scaffold placed on solid surface. The multicellular aggregates of CP5 cells covered the surface of PLA scaffolds and the chondrocytes migrated through and overgrew internal fibers of the scaffolds. Our hybrid culture system simultaneously allows the adhesion of adherent CP5 cells to fibers of PLA scaffolds as well as, due to use of phase of PFC, enhances the mass transfer in the case of supplying/removing of respiratory gases, i.e., $ O_{2} $ and $ CO_{2} $. Our flexible (independent of vessel shape) system is simple, ready-to-use and may utilize a variety of polymer-based scaffolds traditionally proposed for implant development. © The Author(s) 2014 |
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Abstract CP5 bovine chondrocytes were cultured on biodegradable electrospun fibrous polylactide (PLA) scaffolds placed on a flexible interface formed between two immiscible liquid phases: (1) hydrophobic perfluorochemical (PFC) and (2) aqueous culture medium, as a new way of cartilage implant development. Robust and intensive growth of CP5 cells was achieved in our hybrid liquid–solid–liquid culture system consisting of the fibrous PLA scaffolds in contrast to limited growth of the CP5 cells in traditional culture system with PLA scaffold placed on solid surface. The multicellular aggregates of CP5 cells covered the surface of PLA scaffolds and the chondrocytes migrated through and overgrew internal fibers of the scaffolds. Our hybrid culture system simultaneously allows the adhesion of adherent CP5 cells to fibers of PLA scaffolds as well as, due to use of phase of PFC, enhances the mass transfer in the case of supplying/removing of respiratory gases, i.e., $ O_{2} $ and $ CO_{2} $. Our flexible (independent of vessel shape) system is simple, ready-to-use and may utilize a variety of polymer-based scaffolds traditionally proposed for implant development. © The Author(s) 2014 |
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Abstract CP5 bovine chondrocytes were cultured on biodegradable electrospun fibrous polylactide (PLA) scaffolds placed on a flexible interface formed between two immiscible liquid phases: (1) hydrophobic perfluorochemical (PFC) and (2) aqueous culture medium, as a new way of cartilage implant development. Robust and intensive growth of CP5 cells was achieved in our hybrid liquid–solid–liquid culture system consisting of the fibrous PLA scaffolds in contrast to limited growth of the CP5 cells in traditional culture system with PLA scaffold placed on solid surface. The multicellular aggregates of CP5 cells covered the surface of PLA scaffolds and the chondrocytes migrated through and overgrew internal fibers of the scaffolds. Our hybrid culture system simultaneously allows the adhesion of adherent CP5 cells to fibers of PLA scaffolds as well as, due to use of phase of PFC, enhances the mass transfer in the case of supplying/removing of respiratory gases, i.e., $ O_{2} $ and $ CO_{2} $. Our flexible (independent of vessel shape) system is simple, ready-to-use and may utilize a variety of polymer-based scaffolds traditionally proposed for implant development. © The Author(s) 2014 |
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