Feeding strategy design for recombinant human growth hormone production by Bacillus subtilis
pre(subC)::hGH. Defined medium-based feeding strategies were designed by exponential feeding of glucose and ($ NH_{4} $)2$ HPO_{4} $ at two pre-determined specific growth rates, µ0 = 0.10 and 0.17 $ h^{−1} $. Semi-defined medium-based feeding strategies were designed by exponential feeding of substr...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Şahin, Burcu [verfasserIn] |
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| Format: |
Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2015 |
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| Schlagwörter: |
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| Anmerkung: |
© Springer-Verlag Berlin Heidelberg 2015 |
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| Übergeordnetes Werk: |
Enthalten in: Bioprocess and biosystems engineering - Springer Berlin Heidelberg, 2001, 38(2015), 10 vom: 24. Juni, Seite 1855-1865 |
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| Übergeordnetes Werk: |
volume:38 ; year:2015 ; number:10 ; day:24 ; month:06 ; pages:1855-1865 |
| Links: |
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| DOI / URN: |
10.1007/s00449-015-1426-3 |
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| Katalog-ID: |
OLC2106634595 |
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| 520 | |a Abstract Defined and semi-defined medium-based feeding strategies were developed to enhance recombinant human growth hormone (rhGH) production by Bacillus subtilis BGSC-1A178 (scoC−) strain carrying pMK4::pre(subC)::hGH. Defined medium-based feeding strategies were designed by exponential feeding of glucose and ($ NH_{4} $)2$ HPO_{4} $ at two pre-determined specific growth rates, µ0 = 0.10 and 0.17 $ h^{−1} $. Semi-defined medium-based feeding strategies were designed by exponential feeding of substrate solution consisting of glucose, ($ NH_{4} $)2$ HPO_{4} $, peptone, and trace salt solution (PTM1) at three pre-determined specific growth rates, µ0 = 0.10, 0.17, and 0.25 $ h^{−1} $. At all the strategies applied, transition cultivation time from batch to fed-batch operation was tT = 4 h. The highest rhGH concentration was obtained as CrhGH = 0.5 g $ L^{−1} $ with semi-defined medium-based feeding strategy designed with µ0 = 0.25 $ h^{−1} $ using feed substrate stock solution containing 200 g $ L^{−1} $ glucose, 117 g $ L^{−1} $ ($ NH_{4} $)2$ HPO_{4} $, 100 g $ L^{−1} $ peptone, and 5 mL $ L^{−1} $ PTM1 at t = 22 h when the cell concentration reached to CX = 8.29 g $ L^{−1} $. The overall product and cell yields on glucose were obtained as $$\bar{Y}_{P/S}$$ = 7.21 mg $ g^{−1} $ and $$\bar{Y}_{X/S}$$ = 0.12 g $ g^{−1} $, respectively. The results indicate the requirement of designing continuous feed stream in fed-batch production to enhance rhGH production by r-B. subtilis. | ||
| 650 | 4 | |a Fed-batch operation | |
| 650 | 4 | |a Exponential feeding | |
| 650 | 4 | |a Glucose | |
| 650 | 4 | |a Semi-defined medium | |
| 650 | 4 | |a Human growth hormone | |
| 700 | 1 | |a Öztürk, Sibel |4 aut | |
| 700 | 1 | |a Çalık, Pınar |4 aut | |
| 700 | 1 | |a Özdamar, Tunçer H. |4 aut | |
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10.1007/s00449-015-1426-3 doi (DE-627)OLC2106634595 (DE-He213)s00449-015-1426-3-p DE-627 ger DE-627 rakwb eng 660 VZ 570 690 540 VZ 12 ssgn Şahin, Burcu verfasserin aut Feeding strategy design for recombinant human growth hormone production by Bacillus subtilis 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag Berlin Heidelberg 2015 Abstract Defined and semi-defined medium-based feeding strategies were developed to enhance recombinant human growth hormone (rhGH) production by Bacillus subtilis BGSC-1A178 (scoC−) strain carrying pMK4::pre(subC)::hGH. Defined medium-based feeding strategies were designed by exponential feeding of glucose and ($ NH_{4} $)2$ HPO_{4} $ at two pre-determined specific growth rates, µ0 = 0.10 and 0.17 $ h^{−1} $. Semi-defined medium-based feeding strategies were designed by exponential feeding of substrate solution consisting of glucose, ($ NH_{4} $)2$ HPO_{4} $, peptone, and trace salt solution (PTM1) at three pre-determined specific growth rates, µ0 = 0.10, 0.17, and 0.25 $ h^{−1} $. At all the strategies applied, transition cultivation time from batch to fed-batch operation was tT = 4 h. The highest rhGH concentration was obtained as CrhGH = 0.5 g $ L^{−1} $ with semi-defined medium-based feeding strategy designed with µ0 = 0.25 $ h^{−1} $ using feed substrate stock solution containing 200 g $ L^{−1} $ glucose, 117 g $ L^{−1} $ ($ NH_{4} $)2$ HPO_{4} $, 100 g $ L^{−1} $ peptone, and 5 mL $ L^{−1} $ PTM1 at t = 22 h when the cell concentration reached to CX = 8.29 g $ L^{−1} $. The overall product and cell yields on glucose were obtained as $$\bar{Y}_{P/S}$$ = 7.21 mg $ g^{−1} $ and $$\bar{Y}_{X/S}$$ = 0.12 g $ g^{−1} $, respectively. The results indicate the requirement of designing continuous feed stream in fed-batch production to enhance rhGH production by r-B. subtilis. Fed-batch operation Exponential feeding Glucose Semi-defined medium Human growth hormone Öztürk, Sibel aut Çalık, Pınar aut Özdamar, Tunçer H. aut Enthalten in Bioprocess and biosystems engineering Springer Berlin Heidelberg, 2001 38(2015), 10 vom: 24. Juni, Seite 1855-1865 (DE-627)333469763 (DE-600)2056063-1 (DE-576)094533709 1615-7591 nnns volume:38 year:2015 number:10 day:24 month:06 pages:1855-1865 https://doi.org/10.1007/s00449-015-1426-3 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-ARC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_24 GBV_ILN_31 GBV_ILN_70 GBV_ILN_2018 GBV_ILN_4046 GBV_ILN_4277 AR 38 2015 10 24 06 1855-1865 |
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10.1007/s00449-015-1426-3 doi (DE-627)OLC2106634595 (DE-He213)s00449-015-1426-3-p DE-627 ger DE-627 rakwb eng 660 VZ 570 690 540 VZ 12 ssgn Şahin, Burcu verfasserin aut Feeding strategy design for recombinant human growth hormone production by Bacillus subtilis 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag Berlin Heidelberg 2015 Abstract Defined and semi-defined medium-based feeding strategies were developed to enhance recombinant human growth hormone (rhGH) production by Bacillus subtilis BGSC-1A178 (scoC−) strain carrying pMK4::pre(subC)::hGH. Defined medium-based feeding strategies were designed by exponential feeding of glucose and ($ NH_{4} $)2$ HPO_{4} $ at two pre-determined specific growth rates, µ0 = 0.10 and 0.17 $ h^{−1} $. Semi-defined medium-based feeding strategies were designed by exponential feeding of substrate solution consisting of glucose, ($ NH_{4} $)2$ HPO_{4} $, peptone, and trace salt solution (PTM1) at three pre-determined specific growth rates, µ0 = 0.10, 0.17, and 0.25 $ h^{−1} $. At all the strategies applied, transition cultivation time from batch to fed-batch operation was tT = 4 h. The highest rhGH concentration was obtained as CrhGH = 0.5 g $ L^{−1} $ with semi-defined medium-based feeding strategy designed with µ0 = 0.25 $ h^{−1} $ using feed substrate stock solution containing 200 g $ L^{−1} $ glucose, 117 g $ L^{−1} $ ($ NH_{4} $)2$ HPO_{4} $, 100 g $ L^{−1} $ peptone, and 5 mL $ L^{−1} $ PTM1 at t = 22 h when the cell concentration reached to CX = 8.29 g $ L^{−1} $. The overall product and cell yields on glucose were obtained as $$\bar{Y}_{P/S}$$ = 7.21 mg $ g^{−1} $ and $$\bar{Y}_{X/S}$$ = 0.12 g $ g^{−1} $, respectively. The results indicate the requirement of designing continuous feed stream in fed-batch production to enhance rhGH production by r-B. subtilis. Fed-batch operation Exponential feeding Glucose Semi-defined medium Human growth hormone Öztürk, Sibel aut Çalık, Pınar aut Özdamar, Tunçer H. aut Enthalten in Bioprocess and biosystems engineering Springer Berlin Heidelberg, 2001 38(2015), 10 vom: 24. Juni, Seite 1855-1865 (DE-627)333469763 (DE-600)2056063-1 (DE-576)094533709 1615-7591 nnns volume:38 year:2015 number:10 day:24 month:06 pages:1855-1865 https://doi.org/10.1007/s00449-015-1426-3 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-ARC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_24 GBV_ILN_31 GBV_ILN_70 GBV_ILN_2018 GBV_ILN_4046 GBV_ILN_4277 AR 38 2015 10 24 06 1855-1865 |
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10.1007/s00449-015-1426-3 doi (DE-627)OLC2106634595 (DE-He213)s00449-015-1426-3-p DE-627 ger DE-627 rakwb eng 660 VZ 570 690 540 VZ 12 ssgn Şahin, Burcu verfasserin aut Feeding strategy design for recombinant human growth hormone production by Bacillus subtilis 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag Berlin Heidelberg 2015 Abstract Defined and semi-defined medium-based feeding strategies were developed to enhance recombinant human growth hormone (rhGH) production by Bacillus subtilis BGSC-1A178 (scoC−) strain carrying pMK4::pre(subC)::hGH. Defined medium-based feeding strategies were designed by exponential feeding of glucose and ($ NH_{4} $)2$ HPO_{4} $ at two pre-determined specific growth rates, µ0 = 0.10 and 0.17 $ h^{−1} $. Semi-defined medium-based feeding strategies were designed by exponential feeding of substrate solution consisting of glucose, ($ NH_{4} $)2$ HPO_{4} $, peptone, and trace salt solution (PTM1) at three pre-determined specific growth rates, µ0 = 0.10, 0.17, and 0.25 $ h^{−1} $. At all the strategies applied, transition cultivation time from batch to fed-batch operation was tT = 4 h. The highest rhGH concentration was obtained as CrhGH = 0.5 g $ L^{−1} $ with semi-defined medium-based feeding strategy designed with µ0 = 0.25 $ h^{−1} $ using feed substrate stock solution containing 200 g $ L^{−1} $ glucose, 117 g $ L^{−1} $ ($ NH_{4} $)2$ HPO_{4} $, 100 g $ L^{−1} $ peptone, and 5 mL $ L^{−1} $ PTM1 at t = 22 h when the cell concentration reached to CX = 8.29 g $ L^{−1} $. The overall product and cell yields on glucose were obtained as $$\bar{Y}_{P/S}$$ = 7.21 mg $ g^{−1} $ and $$\bar{Y}_{X/S}$$ = 0.12 g $ g^{−1} $, respectively. The results indicate the requirement of designing continuous feed stream in fed-batch production to enhance rhGH production by r-B. subtilis. Fed-batch operation Exponential feeding Glucose Semi-defined medium Human growth hormone Öztürk, Sibel aut Çalık, Pınar aut Özdamar, Tunçer H. aut Enthalten in Bioprocess and biosystems engineering Springer Berlin Heidelberg, 2001 38(2015), 10 vom: 24. Juni, Seite 1855-1865 (DE-627)333469763 (DE-600)2056063-1 (DE-576)094533709 1615-7591 nnns volume:38 year:2015 number:10 day:24 month:06 pages:1855-1865 https://doi.org/10.1007/s00449-015-1426-3 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-ARC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_24 GBV_ILN_31 GBV_ILN_70 GBV_ILN_2018 GBV_ILN_4046 GBV_ILN_4277 AR 38 2015 10 24 06 1855-1865 |
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10.1007/s00449-015-1426-3 doi (DE-627)OLC2106634595 (DE-He213)s00449-015-1426-3-p DE-627 ger DE-627 rakwb eng 660 VZ 570 690 540 VZ 12 ssgn Şahin, Burcu verfasserin aut Feeding strategy design for recombinant human growth hormone production by Bacillus subtilis 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag Berlin Heidelberg 2015 Abstract Defined and semi-defined medium-based feeding strategies were developed to enhance recombinant human growth hormone (rhGH) production by Bacillus subtilis BGSC-1A178 (scoC−) strain carrying pMK4::pre(subC)::hGH. Defined medium-based feeding strategies were designed by exponential feeding of glucose and ($ NH_{4} $)2$ HPO_{4} $ at two pre-determined specific growth rates, µ0 = 0.10 and 0.17 $ h^{−1} $. Semi-defined medium-based feeding strategies were designed by exponential feeding of substrate solution consisting of glucose, ($ NH_{4} $)2$ HPO_{4} $, peptone, and trace salt solution (PTM1) at three pre-determined specific growth rates, µ0 = 0.10, 0.17, and 0.25 $ h^{−1} $. At all the strategies applied, transition cultivation time from batch to fed-batch operation was tT = 4 h. The highest rhGH concentration was obtained as CrhGH = 0.5 g $ L^{−1} $ with semi-defined medium-based feeding strategy designed with µ0 = 0.25 $ h^{−1} $ using feed substrate stock solution containing 200 g $ L^{−1} $ glucose, 117 g $ L^{−1} $ ($ NH_{4} $)2$ HPO_{4} $, 100 g $ L^{−1} $ peptone, and 5 mL $ L^{−1} $ PTM1 at t = 22 h when the cell concentration reached to CX = 8.29 g $ L^{−1} $. The overall product and cell yields on glucose were obtained as $$\bar{Y}_{P/S}$$ = 7.21 mg $ g^{−1} $ and $$\bar{Y}_{X/S}$$ = 0.12 g $ g^{−1} $, respectively. The results indicate the requirement of designing continuous feed stream in fed-batch production to enhance rhGH production by r-B. subtilis. Fed-batch operation Exponential feeding Glucose Semi-defined medium Human growth hormone Öztürk, Sibel aut Çalık, Pınar aut Özdamar, Tunçer H. aut Enthalten in Bioprocess and biosystems engineering Springer Berlin Heidelberg, 2001 38(2015), 10 vom: 24. Juni, Seite 1855-1865 (DE-627)333469763 (DE-600)2056063-1 (DE-576)094533709 1615-7591 nnns volume:38 year:2015 number:10 day:24 month:06 pages:1855-1865 https://doi.org/10.1007/s00449-015-1426-3 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-ARC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_24 GBV_ILN_31 GBV_ILN_70 GBV_ILN_2018 GBV_ILN_4046 GBV_ILN_4277 AR 38 2015 10 24 06 1855-1865 |
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10.1007/s00449-015-1426-3 doi (DE-627)OLC2106634595 (DE-He213)s00449-015-1426-3-p DE-627 ger DE-627 rakwb eng 660 VZ 570 690 540 VZ 12 ssgn Şahin, Burcu verfasserin aut Feeding strategy design for recombinant human growth hormone production by Bacillus subtilis 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag Berlin Heidelberg 2015 Abstract Defined and semi-defined medium-based feeding strategies were developed to enhance recombinant human growth hormone (rhGH) production by Bacillus subtilis BGSC-1A178 (scoC−) strain carrying pMK4::pre(subC)::hGH. Defined medium-based feeding strategies were designed by exponential feeding of glucose and ($ NH_{4} $)2$ HPO_{4} $ at two pre-determined specific growth rates, µ0 = 0.10 and 0.17 $ h^{−1} $. Semi-defined medium-based feeding strategies were designed by exponential feeding of substrate solution consisting of glucose, ($ NH_{4} $)2$ HPO_{4} $, peptone, and trace salt solution (PTM1) at three pre-determined specific growth rates, µ0 = 0.10, 0.17, and 0.25 $ h^{−1} $. At all the strategies applied, transition cultivation time from batch to fed-batch operation was tT = 4 h. The highest rhGH concentration was obtained as CrhGH = 0.5 g $ L^{−1} $ with semi-defined medium-based feeding strategy designed with µ0 = 0.25 $ h^{−1} $ using feed substrate stock solution containing 200 g $ L^{−1} $ glucose, 117 g $ L^{−1} $ ($ NH_{4} $)2$ HPO_{4} $, 100 g $ L^{−1} $ peptone, and 5 mL $ L^{−1} $ PTM1 at t = 22 h when the cell concentration reached to CX = 8.29 g $ L^{−1} $. The overall product and cell yields on glucose were obtained as $$\bar{Y}_{P/S}$$ = 7.21 mg $ g^{−1} $ and $$\bar{Y}_{X/S}$$ = 0.12 g $ g^{−1} $, respectively. The results indicate the requirement of designing continuous feed stream in fed-batch production to enhance rhGH production by r-B. subtilis. Fed-batch operation Exponential feeding Glucose Semi-defined medium Human growth hormone Öztürk, Sibel aut Çalık, Pınar aut Özdamar, Tunçer H. aut Enthalten in Bioprocess and biosystems engineering Springer Berlin Heidelberg, 2001 38(2015), 10 vom: 24. Juni, Seite 1855-1865 (DE-627)333469763 (DE-600)2056063-1 (DE-576)094533709 1615-7591 nnns volume:38 year:2015 number:10 day:24 month:06 pages:1855-1865 https://doi.org/10.1007/s00449-015-1426-3 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-ARC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_24 GBV_ILN_31 GBV_ILN_70 GBV_ILN_2018 GBV_ILN_4046 GBV_ILN_4277 AR 38 2015 10 24 06 1855-1865 |
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Enthalten in Bioprocess and biosystems engineering 38(2015), 10 vom: 24. Juni, Seite 1855-1865 volume:38 year:2015 number:10 day:24 month:06 pages:1855-1865 |
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Defined medium-based feeding strategies were designed by exponential feeding of glucose and ($ NH_{4} $)2$ HPO_{4} $ at two pre-determined specific growth rates, µ0 = 0.10 and 0.17 $ h^{−1} $. Semi-defined medium-based feeding strategies were designed by exponential feeding of substrate solution consisting of glucose, ($ NH_{4} $)2$ HPO_{4} $, peptone, and trace salt solution (PTM1) at three pre-determined specific growth rates, µ0 = 0.10, 0.17, and 0.25 $ h^{−1} $. At all the strategies applied, transition cultivation time from batch to fed-batch operation was tT = 4 h. The highest rhGH concentration was obtained as CrhGH = 0.5 g $ L^{−1} $ with semi-defined medium-based feeding strategy designed with µ0 = 0.25 $ h^{−1} $ using feed substrate stock solution containing 200 g $ L^{−1} $ glucose, 117 g $ L^{−1} $ ($ NH_{4} $)2$ HPO_{4} $, 100 g $ L^{−1} $ peptone, and 5 mL $ L^{−1} $ PTM1 at t = 22 h when the cell concentration reached to CX = 8.29 g $ L^{−1} $. The overall product and cell yields on glucose were obtained as $$\bar{Y}_{P/S}$$ = 7.21 mg $ g^{−1} $ and $$\bar{Y}_{X/S}$$ = 0.12 g $ g^{−1} $, respectively. 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feeding strategy design for recombinant human growth hormone production by bacillus subtilis |
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Feeding strategy design for recombinant human growth hormone production by Bacillus subtilis |
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Abstract Defined and semi-defined medium-based feeding strategies were developed to enhance recombinant human growth hormone (rhGH) production by Bacillus subtilis BGSC-1A178 (scoC−) strain carrying pMK4::pre(subC)::hGH. Defined medium-based feeding strategies were designed by exponential feeding of glucose and ($ NH_{4} $)2$ HPO_{4} $ at two pre-determined specific growth rates, µ0 = 0.10 and 0.17 $ h^{−1} $. Semi-defined medium-based feeding strategies were designed by exponential feeding of substrate solution consisting of glucose, ($ NH_{4} $)2$ HPO_{4} $, peptone, and trace salt solution (PTM1) at three pre-determined specific growth rates, µ0 = 0.10, 0.17, and 0.25 $ h^{−1} $. At all the strategies applied, transition cultivation time from batch to fed-batch operation was tT = 4 h. The highest rhGH concentration was obtained as CrhGH = 0.5 g $ L^{−1} $ with semi-defined medium-based feeding strategy designed with µ0 = 0.25 $ h^{−1} $ using feed substrate stock solution containing 200 g $ L^{−1} $ glucose, 117 g $ L^{−1} $ ($ NH_{4} $)2$ HPO_{4} $, 100 g $ L^{−1} $ peptone, and 5 mL $ L^{−1} $ PTM1 at t = 22 h when the cell concentration reached to CX = 8.29 g $ L^{−1} $. The overall product and cell yields on glucose were obtained as $$\bar{Y}_{P/S}$$ = 7.21 mg $ g^{−1} $ and $$\bar{Y}_{X/S}$$ = 0.12 g $ g^{−1} $, respectively. The results indicate the requirement of designing continuous feed stream in fed-batch production to enhance rhGH production by r-B. subtilis. © Springer-Verlag Berlin Heidelberg 2015 |
| abstractGer |
Abstract Defined and semi-defined medium-based feeding strategies were developed to enhance recombinant human growth hormone (rhGH) production by Bacillus subtilis BGSC-1A178 (scoC−) strain carrying pMK4::pre(subC)::hGH. Defined medium-based feeding strategies were designed by exponential feeding of glucose and ($ NH_{4} $)2$ HPO_{4} $ at two pre-determined specific growth rates, µ0 = 0.10 and 0.17 $ h^{−1} $. Semi-defined medium-based feeding strategies were designed by exponential feeding of substrate solution consisting of glucose, ($ NH_{4} $)2$ HPO_{4} $, peptone, and trace salt solution (PTM1) at three pre-determined specific growth rates, µ0 = 0.10, 0.17, and 0.25 $ h^{−1} $. At all the strategies applied, transition cultivation time from batch to fed-batch operation was tT = 4 h. The highest rhGH concentration was obtained as CrhGH = 0.5 g $ L^{−1} $ with semi-defined medium-based feeding strategy designed with µ0 = 0.25 $ h^{−1} $ using feed substrate stock solution containing 200 g $ L^{−1} $ glucose, 117 g $ L^{−1} $ ($ NH_{4} $)2$ HPO_{4} $, 100 g $ L^{−1} $ peptone, and 5 mL $ L^{−1} $ PTM1 at t = 22 h when the cell concentration reached to CX = 8.29 g $ L^{−1} $. The overall product and cell yields on glucose were obtained as $$\bar{Y}_{P/S}$$ = 7.21 mg $ g^{−1} $ and $$\bar{Y}_{X/S}$$ = 0.12 g $ g^{−1} $, respectively. The results indicate the requirement of designing continuous feed stream in fed-batch production to enhance rhGH production by r-B. subtilis. © Springer-Verlag Berlin Heidelberg 2015 |
| abstract_unstemmed |
Abstract Defined and semi-defined medium-based feeding strategies were developed to enhance recombinant human growth hormone (rhGH) production by Bacillus subtilis BGSC-1A178 (scoC−) strain carrying pMK4::pre(subC)::hGH. Defined medium-based feeding strategies were designed by exponential feeding of glucose and ($ NH_{4} $)2$ HPO_{4} $ at two pre-determined specific growth rates, µ0 = 0.10 and 0.17 $ h^{−1} $. Semi-defined medium-based feeding strategies were designed by exponential feeding of substrate solution consisting of glucose, ($ NH_{4} $)2$ HPO_{4} $, peptone, and trace salt solution (PTM1) at three pre-determined specific growth rates, µ0 = 0.10, 0.17, and 0.25 $ h^{−1} $. At all the strategies applied, transition cultivation time from batch to fed-batch operation was tT = 4 h. The highest rhGH concentration was obtained as CrhGH = 0.5 g $ L^{−1} $ with semi-defined medium-based feeding strategy designed with µ0 = 0.25 $ h^{−1} $ using feed substrate stock solution containing 200 g $ L^{−1} $ glucose, 117 g $ L^{−1} $ ($ NH_{4} $)2$ HPO_{4} $, 100 g $ L^{−1} $ peptone, and 5 mL $ L^{−1} $ PTM1 at t = 22 h when the cell concentration reached to CX = 8.29 g $ L^{−1} $. The overall product and cell yields on glucose were obtained as $$\bar{Y}_{P/S}$$ = 7.21 mg $ g^{−1} $ and $$\bar{Y}_{X/S}$$ = 0.12 g $ g^{−1} $, respectively. The results indicate the requirement of designing continuous feed stream in fed-batch production to enhance rhGH production by r-B. subtilis. © Springer-Verlag Berlin Heidelberg 2015 |
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