Enzymatic synthesis of phytosterol esters catalyzed by Candida rugosa lipase in water-in-[Bmim]$ PF_{6} $ microemulsion
Abstract A water-in-ionic liquid microemulsion ([Bmim]$ PF_{6} $/Tween20/$ H_{2} $O) was applied as reusable reaction medium to esterify phytosterols with fatty acid by Candida rugosa lipase (CRL) successfully. Two kinds of commercial CRLs, AY30 and AYS which cannot effectively catalyze esterificati...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Zeng, Chaoxi [verfasserIn] |
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| Format: |
Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2015 |
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| Schlagwörter: |
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| Anmerkung: |
© Springer-Verlag Berlin Heidelberg 2015 |
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| Übergeordnetes Werk: |
Enthalten in: Bioprocess and biosystems engineering - Springer Berlin Heidelberg, 2001, 38(2015), 5 vom: 10. Jan., Seite 939-946 |
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| Übergeordnetes Werk: |
volume:38 ; year:2015 ; number:5 ; day:10 ; month:01 ; pages:939-946 |
| Links: |
|---|
| DOI / URN: |
10.1007/s00449-014-1339-6 |
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| Katalog-ID: |
OLC210663725X |
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| 245 | 1 | 0 | |a Enzymatic synthesis of phytosterol esters catalyzed by Candida rugosa lipase in water-in-[Bmim]$ PF_{6} $ microemulsion |
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| 520 | |a Abstract A water-in-ionic liquid microemulsion ([Bmim]$ PF_{6} $/Tween20/$ H_{2} $O) was applied as reusable reaction medium to esterify phytosterols with fatty acid by Candida rugosa lipase (CRL) successfully. Two kinds of commercial CRLs, AY30 and AYS which cannot effectively catalyze esterification in conventional reaction system were found effective in the microemulsion system. Effects of reaction parameters on esterification were investigated; results showed that the conversion rate of 87.9 and 95.1 % was obtained in 24 and 48 h of reaction, respectively, under the optimized condition: the molar ratio of water to Tween 20 (w0 value) at 5.4, Tween 20 at a concentration of 305 mM, 50 °C,pH 7.4, 10 % of enzyme loading (w/w, with respect to total reactants), and phytosterols/lauric acid molar ratio of 1:2. Moreover, by using n-hexane as the extraction agent, the lipase-encapsulated microemulsion could be reused at least seven times (>168 h) without significant changes in the conversion rate, while achieving a purpose of simple separation and purification. | ||
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| 700 | 1 | |a Yang, Bo |4 aut | |
| 700 | 1 | |a Wang, Yonghua |4 aut | |
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10.1007/s00449-014-1339-6 doi (DE-627)OLC210663725X (DE-He213)s00449-014-1339-6-p DE-627 ger DE-627 rakwb eng 660 VZ 570 690 540 VZ 12 ssgn Zeng, Chaoxi verfasserin aut Enzymatic synthesis of phytosterol esters catalyzed by Candida rugosa lipase in water-in-[Bmim]$ PF_{6} $ microemulsion 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag Berlin Heidelberg 2015 Abstract A water-in-ionic liquid microemulsion ([Bmim]$ PF_{6} $/Tween20/$ H_{2} $O) was applied as reusable reaction medium to esterify phytosterols with fatty acid by Candida rugosa lipase (CRL) successfully. Two kinds of commercial CRLs, AY30 and AYS which cannot effectively catalyze esterification in conventional reaction system were found effective in the microemulsion system. Effects of reaction parameters on esterification were investigated; results showed that the conversion rate of 87.9 and 95.1 % was obtained in 24 and 48 h of reaction, respectively, under the optimized condition: the molar ratio of water to Tween 20 (w0 value) at 5.4, Tween 20 at a concentration of 305 mM, 50 °C,pH 7.4, 10 % of enzyme loading (w/w, with respect to total reactants), and phytosterols/lauric acid molar ratio of 1:2. Moreover, by using n-hexane as the extraction agent, the lipase-encapsulated microemulsion could be reused at least seven times (>168 h) without significant changes in the conversion rate, while achieving a purpose of simple separation and purification. Phytosterols Phytosterol ester Lipase Ionic liquid Microemulsion Qi, Suijian aut Li, Zhigang aut Luo, Riming aut Yang, Bo aut Wang, Yonghua aut Enthalten in Bioprocess and biosystems engineering Springer Berlin Heidelberg, 2001 38(2015), 5 vom: 10. Jan., Seite 939-946 (DE-627)333469763 (DE-600)2056063-1 (DE-576)094533709 1615-7591 nnns volume:38 year:2015 number:5 day:10 month:01 pages:939-946 https://doi.org/10.1007/s00449-014-1339-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-ARC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_24 GBV_ILN_31 GBV_ILN_70 GBV_ILN_2018 GBV_ILN_4046 GBV_ILN_4277 AR 38 2015 5 10 01 939-946 |
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10.1007/s00449-014-1339-6 doi (DE-627)OLC210663725X (DE-He213)s00449-014-1339-6-p DE-627 ger DE-627 rakwb eng 660 VZ 570 690 540 VZ 12 ssgn Zeng, Chaoxi verfasserin aut Enzymatic synthesis of phytosterol esters catalyzed by Candida rugosa lipase in water-in-[Bmim]$ PF_{6} $ microemulsion 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag Berlin Heidelberg 2015 Abstract A water-in-ionic liquid microemulsion ([Bmim]$ PF_{6} $/Tween20/$ H_{2} $O) was applied as reusable reaction medium to esterify phytosterols with fatty acid by Candida rugosa lipase (CRL) successfully. Two kinds of commercial CRLs, AY30 and AYS which cannot effectively catalyze esterification in conventional reaction system were found effective in the microemulsion system. Effects of reaction parameters on esterification were investigated; results showed that the conversion rate of 87.9 and 95.1 % was obtained in 24 and 48 h of reaction, respectively, under the optimized condition: the molar ratio of water to Tween 20 (w0 value) at 5.4, Tween 20 at a concentration of 305 mM, 50 °C,pH 7.4, 10 % of enzyme loading (w/w, with respect to total reactants), and phytosterols/lauric acid molar ratio of 1:2. Moreover, by using n-hexane as the extraction agent, the lipase-encapsulated microemulsion could be reused at least seven times (>168 h) without significant changes in the conversion rate, while achieving a purpose of simple separation and purification. Phytosterols Phytosterol ester Lipase Ionic liquid Microemulsion Qi, Suijian aut Li, Zhigang aut Luo, Riming aut Yang, Bo aut Wang, Yonghua aut Enthalten in Bioprocess and biosystems engineering Springer Berlin Heidelberg, 2001 38(2015), 5 vom: 10. Jan., Seite 939-946 (DE-627)333469763 (DE-600)2056063-1 (DE-576)094533709 1615-7591 nnns volume:38 year:2015 number:5 day:10 month:01 pages:939-946 https://doi.org/10.1007/s00449-014-1339-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-ARC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_24 GBV_ILN_31 GBV_ILN_70 GBV_ILN_2018 GBV_ILN_4046 GBV_ILN_4277 AR 38 2015 5 10 01 939-946 |
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10.1007/s00449-014-1339-6 doi (DE-627)OLC210663725X (DE-He213)s00449-014-1339-6-p DE-627 ger DE-627 rakwb eng 660 VZ 570 690 540 VZ 12 ssgn Zeng, Chaoxi verfasserin aut Enzymatic synthesis of phytosterol esters catalyzed by Candida rugosa lipase in water-in-[Bmim]$ PF_{6} $ microemulsion 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag Berlin Heidelberg 2015 Abstract A water-in-ionic liquid microemulsion ([Bmim]$ PF_{6} $/Tween20/$ H_{2} $O) was applied as reusable reaction medium to esterify phytosterols with fatty acid by Candida rugosa lipase (CRL) successfully. Two kinds of commercial CRLs, AY30 and AYS which cannot effectively catalyze esterification in conventional reaction system were found effective in the microemulsion system. Effects of reaction parameters on esterification were investigated; results showed that the conversion rate of 87.9 and 95.1 % was obtained in 24 and 48 h of reaction, respectively, under the optimized condition: the molar ratio of water to Tween 20 (w0 value) at 5.4, Tween 20 at a concentration of 305 mM, 50 °C,pH 7.4, 10 % of enzyme loading (w/w, with respect to total reactants), and phytosterols/lauric acid molar ratio of 1:2. Moreover, by using n-hexane as the extraction agent, the lipase-encapsulated microemulsion could be reused at least seven times (>168 h) without significant changes in the conversion rate, while achieving a purpose of simple separation and purification. Phytosterols Phytosterol ester Lipase Ionic liquid Microemulsion Qi, Suijian aut Li, Zhigang aut Luo, Riming aut Yang, Bo aut Wang, Yonghua aut Enthalten in Bioprocess and biosystems engineering Springer Berlin Heidelberg, 2001 38(2015), 5 vom: 10. Jan., Seite 939-946 (DE-627)333469763 (DE-600)2056063-1 (DE-576)094533709 1615-7591 nnns volume:38 year:2015 number:5 day:10 month:01 pages:939-946 https://doi.org/10.1007/s00449-014-1339-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-ARC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_24 GBV_ILN_31 GBV_ILN_70 GBV_ILN_2018 GBV_ILN_4046 GBV_ILN_4277 AR 38 2015 5 10 01 939-946 |
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10.1007/s00449-014-1339-6 doi (DE-627)OLC210663725X (DE-He213)s00449-014-1339-6-p DE-627 ger DE-627 rakwb eng 660 VZ 570 690 540 VZ 12 ssgn Zeng, Chaoxi verfasserin aut Enzymatic synthesis of phytosterol esters catalyzed by Candida rugosa lipase in water-in-[Bmim]$ PF_{6} $ microemulsion 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag Berlin Heidelberg 2015 Abstract A water-in-ionic liquid microemulsion ([Bmim]$ PF_{6} $/Tween20/$ H_{2} $O) was applied as reusable reaction medium to esterify phytosterols with fatty acid by Candida rugosa lipase (CRL) successfully. Two kinds of commercial CRLs, AY30 and AYS which cannot effectively catalyze esterification in conventional reaction system were found effective in the microemulsion system. Effects of reaction parameters on esterification were investigated; results showed that the conversion rate of 87.9 and 95.1 % was obtained in 24 and 48 h of reaction, respectively, under the optimized condition: the molar ratio of water to Tween 20 (w0 value) at 5.4, Tween 20 at a concentration of 305 mM, 50 °C,pH 7.4, 10 % of enzyme loading (w/w, with respect to total reactants), and phytosterols/lauric acid molar ratio of 1:2. Moreover, by using n-hexane as the extraction agent, the lipase-encapsulated microemulsion could be reused at least seven times (>168 h) without significant changes in the conversion rate, while achieving a purpose of simple separation and purification. Phytosterols Phytosterol ester Lipase Ionic liquid Microemulsion Qi, Suijian aut Li, Zhigang aut Luo, Riming aut Yang, Bo aut Wang, Yonghua aut Enthalten in Bioprocess and biosystems engineering Springer Berlin Heidelberg, 2001 38(2015), 5 vom: 10. Jan., Seite 939-946 (DE-627)333469763 (DE-600)2056063-1 (DE-576)094533709 1615-7591 nnns volume:38 year:2015 number:5 day:10 month:01 pages:939-946 https://doi.org/10.1007/s00449-014-1339-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-ARC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_24 GBV_ILN_31 GBV_ILN_70 GBV_ILN_2018 GBV_ILN_4046 GBV_ILN_4277 AR 38 2015 5 10 01 939-946 |
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Enthalten in Bioprocess and biosystems engineering 38(2015), 5 vom: 10. Jan., Seite 939-946 volume:38 year:2015 number:5 day:10 month:01 pages:939-946 |
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Zeng, Chaoxi |
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660 VZ 570 690 540 VZ 12 ssgn Enzymatic synthesis of phytosterol esters catalyzed by Candida rugosa lipase in water-in-[Bmim]$ PF_{6} $ microemulsion Phytosterols Phytosterol ester Lipase Ionic liquid Microemulsion |
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ddc 660 ddc 570 ssgn 12 misc Phytosterols misc Phytosterol ester misc Lipase misc Ionic liquid misc Microemulsion |
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Enzymatic synthesis of phytosterol esters catalyzed by Candida rugosa lipase in water-in-[Bmim]$ PF_{6} $ microemulsion |
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Enzymatic synthesis of phytosterol esters catalyzed by Candida rugosa lipase in water-in-[Bmim]$ PF_{6} $ microemulsion |
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Zeng, Chaoxi |
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Bioprocess and biosystems engineering |
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Zeng, Chaoxi Qi, Suijian Li, Zhigang Luo, Riming Yang, Bo Wang, Yonghua |
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enzymatic synthesis of phytosterol esters catalyzed by candida rugosa lipase in water-in-[bmim]$ pf_{6} $ microemulsion |
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Enzymatic synthesis of phytosterol esters catalyzed by Candida rugosa lipase in water-in-[Bmim]$ PF_{6} $ microemulsion |
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Abstract A water-in-ionic liquid microemulsion ([Bmim]$ PF_{6} $/Tween20/$ H_{2} $O) was applied as reusable reaction medium to esterify phytosterols with fatty acid by Candida rugosa lipase (CRL) successfully. Two kinds of commercial CRLs, AY30 and AYS which cannot effectively catalyze esterification in conventional reaction system were found effective in the microemulsion system. Effects of reaction parameters on esterification were investigated; results showed that the conversion rate of 87.9 and 95.1 % was obtained in 24 and 48 h of reaction, respectively, under the optimized condition: the molar ratio of water to Tween 20 (w0 value) at 5.4, Tween 20 at a concentration of 305 mM, 50 °C,pH 7.4, 10 % of enzyme loading (w/w, with respect to total reactants), and phytosterols/lauric acid molar ratio of 1:2. Moreover, by using n-hexane as the extraction agent, the lipase-encapsulated microemulsion could be reused at least seven times (>168 h) without significant changes in the conversion rate, while achieving a purpose of simple separation and purification. © Springer-Verlag Berlin Heidelberg 2015 |
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Abstract A water-in-ionic liquid microemulsion ([Bmim]$ PF_{6} $/Tween20/$ H_{2} $O) was applied as reusable reaction medium to esterify phytosterols with fatty acid by Candida rugosa lipase (CRL) successfully. Two kinds of commercial CRLs, AY30 and AYS which cannot effectively catalyze esterification in conventional reaction system were found effective in the microemulsion system. Effects of reaction parameters on esterification were investigated; results showed that the conversion rate of 87.9 and 95.1 % was obtained in 24 and 48 h of reaction, respectively, under the optimized condition: the molar ratio of water to Tween 20 (w0 value) at 5.4, Tween 20 at a concentration of 305 mM, 50 °C,pH 7.4, 10 % of enzyme loading (w/w, with respect to total reactants), and phytosterols/lauric acid molar ratio of 1:2. Moreover, by using n-hexane as the extraction agent, the lipase-encapsulated microemulsion could be reused at least seven times (>168 h) without significant changes in the conversion rate, while achieving a purpose of simple separation and purification. © Springer-Verlag Berlin Heidelberg 2015 |
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Abstract A water-in-ionic liquid microemulsion ([Bmim]$ PF_{6} $/Tween20/$ H_{2} $O) was applied as reusable reaction medium to esterify phytosterols with fatty acid by Candida rugosa lipase (CRL) successfully. Two kinds of commercial CRLs, AY30 and AYS which cannot effectively catalyze esterification in conventional reaction system were found effective in the microemulsion system. Effects of reaction parameters on esterification were investigated; results showed that the conversion rate of 87.9 and 95.1 % was obtained in 24 and 48 h of reaction, respectively, under the optimized condition: the molar ratio of water to Tween 20 (w0 value) at 5.4, Tween 20 at a concentration of 305 mM, 50 °C,pH 7.4, 10 % of enzyme loading (w/w, with respect to total reactants), and phytosterols/lauric acid molar ratio of 1:2. Moreover, by using n-hexane as the extraction agent, the lipase-encapsulated microemulsion could be reused at least seven times (>168 h) without significant changes in the conversion rate, while achieving a purpose of simple separation and purification. © Springer-Verlag Berlin Heidelberg 2015 |
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Enzymatic synthesis of phytosterol esters catalyzed by Candida rugosa lipase in water-in-[Bmim]$ PF_{6} $ microemulsion |
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