The growth of Pichia pastoris $ Mut^{+} $ on methanol–glycerol mixtures fits to interactive dual-limited kinetics: model development and application to optimised fed-batch operation for heterologous protein production
Abstract The methanol–glycerol co-feeding during the induction stage for heterologous protein production in Pichia pastoris has shown significant productive applications. Available model analysis applied to this dual-limited condition is scarce and normally does not consider the interaction effects...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Canales, Christian [verfasserIn] |
|---|
| Format: |
Artikel |
|---|---|
| Sprache: |
Englisch |
| Erschienen: |
2018 |
|---|
| Schlagwörter: |
Additive-enhancing kinetic model Heterologous protein production |
|---|
| Anmerkung: |
© Springer-Verlag GmbH Germany, part of Springer Nature 2018 |
|---|
| Übergeordnetes Werk: |
Enthalten in: Bioprocess and biosystems engineering - Springer Berlin Heidelberg, 2001, 41(2018), 12 vom: 08. Sept., Seite 1827-1838 |
|---|---|
| Übergeordnetes Werk: |
volume:41 ; year:2018 ; number:12 ; day:08 ; month:09 ; pages:1827-1838 |
| Links: |
|---|
| DOI / URN: |
10.1007/s00449-018-2005-1 |
|---|
| Katalog-ID: |
OLC2106647050 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | OLC2106647050 | ||
| 003 | DE-627 | ||
| 005 | 20230403073534.0 | ||
| 007 | tu | ||
| 008 | 230403s2018 xx ||||| 00| ||eng c | ||
| 024 | 7 | |a 10.1007/s00449-018-2005-1 |2 doi | |
| 035 | |a (DE-627)OLC2106647050 | ||
| 035 | |a (DE-He213)s00449-018-2005-1-p | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 082 | 0 | 4 | |a 660 |q VZ |
| 082 | 0 | 4 | |a 570 |a 690 |a 540 |q VZ |
| 084 | |a 12 |2 ssgn | ||
| 100 | 1 | |a Canales, Christian |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a The growth of Pichia pastoris $ Mut^{+} $ on methanol–glycerol mixtures fits to interactive dual-limited kinetics: model development and application to optimised fed-batch operation for heterologous protein production |
| 264 | 1 | |c 2018 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a © Springer-Verlag GmbH Germany, part of Springer Nature 2018 | ||
| 520 | |a Abstract The methanol–glycerol co-feeding during the induction stage for heterologous protein production in Pichia pastoris has shown significant productive applications. Available model analysis applied to this dual-limited condition is scarce and normally does not consider the interaction effects between the substrates. In this work, a dual-limited growth model of P. pastoris considering an interactive kinetic effect was applied to an optimised fed-batch process production of heterologous Rhizopus oryzae lipase (ROL). In the proposed model, the growth kinetics on glycerol is fully expressed, whereas methanol kinetics is modulated by the co-metabolisation of glycerol, resulting in an enhancing effect of glycerol-specific growth rate. The modelling approach of fed-batch cultures also included the methanol volatilisation caused by the aeration that was found to be a not-negligible phenomenon. The model predicts the ability of P. pastoris to keep control of the methanol concentration in the broth during ROL-optimised production process in fed batch and fits satisfactorily the specific cell growth rate and ROL production. Implications of interaction effect are discussed applying the general procedure of modelling approach. | ||
| 650 | 4 | |a Dual-limited growth modelling | |
| 650 | 4 | |a Additive-enhancing kinetic model | |
| 650 | 4 | |a Heterologous protein production | |
| 650 | 4 | |a Methanol–glycerol mixed feeding | |
| 650 | 4 | |a Fed-batch exponential feeding | |
| 700 | 1 | |a Altamirano, Claudia |4 aut | |
| 700 | 1 | |a Berrios, Julio |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Bioprocess and biosystems engineering |d Springer Berlin Heidelberg, 2001 |g 41(2018), 12 vom: 08. Sept., Seite 1827-1838 |w (DE-627)333469763 |w (DE-600)2056063-1 |w (DE-576)094533709 |x 1615-7591 |7 nnns |
| 773 | 1 | 8 | |g volume:41 |g year:2018 |g number:12 |g day:08 |g month:09 |g pages:1827-1838 |
| 856 | 4 | 1 | |u https://doi.org/10.1007/s00449-018-2005-1 |z lizenzpflichtig |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a SYSFLAG_A | ||
| 912 | |a GBV_OLC | ||
| 912 | |a SSG-OLC-UMW | ||
| 912 | |a SSG-OLC-ARC | ||
| 912 | |a SSG-OLC-TEC | ||
| 912 | |a SSG-OLC-CHE | ||
| 912 | |a GBV_ILN_24 | ||
| 912 | |a GBV_ILN_31 | ||
| 912 | |a GBV_ILN_70 | ||
| 912 | |a GBV_ILN_2018 | ||
| 912 | |a GBV_ILN_4277 | ||
| 951 | |a AR | ||
| 952 | |d 41 |j 2018 |e 12 |b 08 |c 09 |h 1827-1838 | ||
| author_variant |
c c cc c a ca j b jb |
|---|---|
| matchkey_str |
article:16157591:2018----::hgotopcipsoimtnehnllcrlitrsisoneatvdalmtdieisoedvlpetnapiainopiiefd |
| hierarchy_sort_str |
2018 |
| publishDate |
2018 |
| allfields |
10.1007/s00449-018-2005-1 doi (DE-627)OLC2106647050 (DE-He213)s00449-018-2005-1-p DE-627 ger DE-627 rakwb eng 660 VZ 570 690 540 VZ 12 ssgn Canales, Christian verfasserin aut The growth of Pichia pastoris $ Mut^{+} $ on methanol–glycerol mixtures fits to interactive dual-limited kinetics: model development and application to optimised fed-batch operation for heterologous protein production 2018 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag GmbH Germany, part of Springer Nature 2018 Abstract The methanol–glycerol co-feeding during the induction stage for heterologous protein production in Pichia pastoris has shown significant productive applications. Available model analysis applied to this dual-limited condition is scarce and normally does not consider the interaction effects between the substrates. In this work, a dual-limited growth model of P. pastoris considering an interactive kinetic effect was applied to an optimised fed-batch process production of heterologous Rhizopus oryzae lipase (ROL). In the proposed model, the growth kinetics on glycerol is fully expressed, whereas methanol kinetics is modulated by the co-metabolisation of glycerol, resulting in an enhancing effect of glycerol-specific growth rate. The modelling approach of fed-batch cultures also included the methanol volatilisation caused by the aeration that was found to be a not-negligible phenomenon. The model predicts the ability of P. pastoris to keep control of the methanol concentration in the broth during ROL-optimised production process in fed batch and fits satisfactorily the specific cell growth rate and ROL production. Implications of interaction effect are discussed applying the general procedure of modelling approach. Dual-limited growth modelling Additive-enhancing kinetic model Heterologous protein production Methanol–glycerol mixed feeding Fed-batch exponential feeding Altamirano, Claudia aut Berrios, Julio aut Enthalten in Bioprocess and biosystems engineering Springer Berlin Heidelberg, 2001 41(2018), 12 vom: 08. Sept., Seite 1827-1838 (DE-627)333469763 (DE-600)2056063-1 (DE-576)094533709 1615-7591 nnns volume:41 year:2018 number:12 day:08 month:09 pages:1827-1838 https://doi.org/10.1007/s00449-018-2005-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-ARC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_24 GBV_ILN_31 GBV_ILN_70 GBV_ILN_2018 GBV_ILN_4277 AR 41 2018 12 08 09 1827-1838 |
| spelling |
10.1007/s00449-018-2005-1 doi (DE-627)OLC2106647050 (DE-He213)s00449-018-2005-1-p DE-627 ger DE-627 rakwb eng 660 VZ 570 690 540 VZ 12 ssgn Canales, Christian verfasserin aut The growth of Pichia pastoris $ Mut^{+} $ on methanol–glycerol mixtures fits to interactive dual-limited kinetics: model development and application to optimised fed-batch operation for heterologous protein production 2018 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag GmbH Germany, part of Springer Nature 2018 Abstract The methanol–glycerol co-feeding during the induction stage for heterologous protein production in Pichia pastoris has shown significant productive applications. Available model analysis applied to this dual-limited condition is scarce and normally does not consider the interaction effects between the substrates. In this work, a dual-limited growth model of P. pastoris considering an interactive kinetic effect was applied to an optimised fed-batch process production of heterologous Rhizopus oryzae lipase (ROL). In the proposed model, the growth kinetics on glycerol is fully expressed, whereas methanol kinetics is modulated by the co-metabolisation of glycerol, resulting in an enhancing effect of glycerol-specific growth rate. The modelling approach of fed-batch cultures also included the methanol volatilisation caused by the aeration that was found to be a not-negligible phenomenon. The model predicts the ability of P. pastoris to keep control of the methanol concentration in the broth during ROL-optimised production process in fed batch and fits satisfactorily the specific cell growth rate and ROL production. Implications of interaction effect are discussed applying the general procedure of modelling approach. Dual-limited growth modelling Additive-enhancing kinetic model Heterologous protein production Methanol–glycerol mixed feeding Fed-batch exponential feeding Altamirano, Claudia aut Berrios, Julio aut Enthalten in Bioprocess and biosystems engineering Springer Berlin Heidelberg, 2001 41(2018), 12 vom: 08. Sept., Seite 1827-1838 (DE-627)333469763 (DE-600)2056063-1 (DE-576)094533709 1615-7591 nnns volume:41 year:2018 number:12 day:08 month:09 pages:1827-1838 https://doi.org/10.1007/s00449-018-2005-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-ARC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_24 GBV_ILN_31 GBV_ILN_70 GBV_ILN_2018 GBV_ILN_4277 AR 41 2018 12 08 09 1827-1838 |
| allfields_unstemmed |
10.1007/s00449-018-2005-1 doi (DE-627)OLC2106647050 (DE-He213)s00449-018-2005-1-p DE-627 ger DE-627 rakwb eng 660 VZ 570 690 540 VZ 12 ssgn Canales, Christian verfasserin aut The growth of Pichia pastoris $ Mut^{+} $ on methanol–glycerol mixtures fits to interactive dual-limited kinetics: model development and application to optimised fed-batch operation for heterologous protein production 2018 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag GmbH Germany, part of Springer Nature 2018 Abstract The methanol–glycerol co-feeding during the induction stage for heterologous protein production in Pichia pastoris has shown significant productive applications. Available model analysis applied to this dual-limited condition is scarce and normally does not consider the interaction effects between the substrates. In this work, a dual-limited growth model of P. pastoris considering an interactive kinetic effect was applied to an optimised fed-batch process production of heterologous Rhizopus oryzae lipase (ROL). In the proposed model, the growth kinetics on glycerol is fully expressed, whereas methanol kinetics is modulated by the co-metabolisation of glycerol, resulting in an enhancing effect of glycerol-specific growth rate. The modelling approach of fed-batch cultures also included the methanol volatilisation caused by the aeration that was found to be a not-negligible phenomenon. The model predicts the ability of P. pastoris to keep control of the methanol concentration in the broth during ROL-optimised production process in fed batch and fits satisfactorily the specific cell growth rate and ROL production. Implications of interaction effect are discussed applying the general procedure of modelling approach. Dual-limited growth modelling Additive-enhancing kinetic model Heterologous protein production Methanol–glycerol mixed feeding Fed-batch exponential feeding Altamirano, Claudia aut Berrios, Julio aut Enthalten in Bioprocess and biosystems engineering Springer Berlin Heidelberg, 2001 41(2018), 12 vom: 08. Sept., Seite 1827-1838 (DE-627)333469763 (DE-600)2056063-1 (DE-576)094533709 1615-7591 nnns volume:41 year:2018 number:12 day:08 month:09 pages:1827-1838 https://doi.org/10.1007/s00449-018-2005-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-ARC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_24 GBV_ILN_31 GBV_ILN_70 GBV_ILN_2018 GBV_ILN_4277 AR 41 2018 12 08 09 1827-1838 |
| allfieldsGer |
10.1007/s00449-018-2005-1 doi (DE-627)OLC2106647050 (DE-He213)s00449-018-2005-1-p DE-627 ger DE-627 rakwb eng 660 VZ 570 690 540 VZ 12 ssgn Canales, Christian verfasserin aut The growth of Pichia pastoris $ Mut^{+} $ on methanol–glycerol mixtures fits to interactive dual-limited kinetics: model development and application to optimised fed-batch operation for heterologous protein production 2018 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag GmbH Germany, part of Springer Nature 2018 Abstract The methanol–glycerol co-feeding during the induction stage for heterologous protein production in Pichia pastoris has shown significant productive applications. Available model analysis applied to this dual-limited condition is scarce and normally does not consider the interaction effects between the substrates. In this work, a dual-limited growth model of P. pastoris considering an interactive kinetic effect was applied to an optimised fed-batch process production of heterologous Rhizopus oryzae lipase (ROL). In the proposed model, the growth kinetics on glycerol is fully expressed, whereas methanol kinetics is modulated by the co-metabolisation of glycerol, resulting in an enhancing effect of glycerol-specific growth rate. The modelling approach of fed-batch cultures also included the methanol volatilisation caused by the aeration that was found to be a not-negligible phenomenon. The model predicts the ability of P. pastoris to keep control of the methanol concentration in the broth during ROL-optimised production process in fed batch and fits satisfactorily the specific cell growth rate and ROL production. Implications of interaction effect are discussed applying the general procedure of modelling approach. Dual-limited growth modelling Additive-enhancing kinetic model Heterologous protein production Methanol–glycerol mixed feeding Fed-batch exponential feeding Altamirano, Claudia aut Berrios, Julio aut Enthalten in Bioprocess and biosystems engineering Springer Berlin Heidelberg, 2001 41(2018), 12 vom: 08. Sept., Seite 1827-1838 (DE-627)333469763 (DE-600)2056063-1 (DE-576)094533709 1615-7591 nnns volume:41 year:2018 number:12 day:08 month:09 pages:1827-1838 https://doi.org/10.1007/s00449-018-2005-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-ARC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_24 GBV_ILN_31 GBV_ILN_70 GBV_ILN_2018 GBV_ILN_4277 AR 41 2018 12 08 09 1827-1838 |
| allfieldsSound |
10.1007/s00449-018-2005-1 doi (DE-627)OLC2106647050 (DE-He213)s00449-018-2005-1-p DE-627 ger DE-627 rakwb eng 660 VZ 570 690 540 VZ 12 ssgn Canales, Christian verfasserin aut The growth of Pichia pastoris $ Mut^{+} $ on methanol–glycerol mixtures fits to interactive dual-limited kinetics: model development and application to optimised fed-batch operation for heterologous protein production 2018 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag GmbH Germany, part of Springer Nature 2018 Abstract The methanol–glycerol co-feeding during the induction stage for heterologous protein production in Pichia pastoris has shown significant productive applications. Available model analysis applied to this dual-limited condition is scarce and normally does not consider the interaction effects between the substrates. In this work, a dual-limited growth model of P. pastoris considering an interactive kinetic effect was applied to an optimised fed-batch process production of heterologous Rhizopus oryzae lipase (ROL). In the proposed model, the growth kinetics on glycerol is fully expressed, whereas methanol kinetics is modulated by the co-metabolisation of glycerol, resulting in an enhancing effect of glycerol-specific growth rate. The modelling approach of fed-batch cultures also included the methanol volatilisation caused by the aeration that was found to be a not-negligible phenomenon. The model predicts the ability of P. pastoris to keep control of the methanol concentration in the broth during ROL-optimised production process in fed batch and fits satisfactorily the specific cell growth rate and ROL production. Implications of interaction effect are discussed applying the general procedure of modelling approach. Dual-limited growth modelling Additive-enhancing kinetic model Heterologous protein production Methanol–glycerol mixed feeding Fed-batch exponential feeding Altamirano, Claudia aut Berrios, Julio aut Enthalten in Bioprocess and biosystems engineering Springer Berlin Heidelberg, 2001 41(2018), 12 vom: 08. Sept., Seite 1827-1838 (DE-627)333469763 (DE-600)2056063-1 (DE-576)094533709 1615-7591 nnns volume:41 year:2018 number:12 day:08 month:09 pages:1827-1838 https://doi.org/10.1007/s00449-018-2005-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-ARC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_24 GBV_ILN_31 GBV_ILN_70 GBV_ILN_2018 GBV_ILN_4277 AR 41 2018 12 08 09 1827-1838 |
| language |
English |
| source |
Enthalten in Bioprocess and biosystems engineering 41(2018), 12 vom: 08. Sept., Seite 1827-1838 volume:41 year:2018 number:12 day:08 month:09 pages:1827-1838 |
| sourceStr |
Enthalten in Bioprocess and biosystems engineering 41(2018), 12 vom: 08. Sept., Seite 1827-1838 volume:41 year:2018 number:12 day:08 month:09 pages:1827-1838 |
| format_phy_str_mv |
Article |
| institution |
findex.gbv.de |
| topic_facet |
Dual-limited growth modelling Additive-enhancing kinetic model Heterologous protein production Methanol–glycerol mixed feeding Fed-batch exponential feeding |
| dewey-raw |
660 |
| isfreeaccess_bool |
false |
| container_title |
Bioprocess and biosystems engineering |
| authorswithroles_txt_mv |
Canales, Christian @@aut@@ Altamirano, Claudia @@aut@@ Berrios, Julio @@aut@@ |
| publishDateDaySort_date |
2018-09-08T00:00:00Z |
| hierarchy_top_id |
333469763 |
| dewey-sort |
3660 |
| id |
OLC2106647050 |
| language_de |
englisch |
| fullrecord |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000naa a22002652 4500</leader><controlfield tag="001">OLC2106647050</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230403073534.0</controlfield><controlfield tag="007">tu</controlfield><controlfield tag="008">230403s2018 xx ||||| 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/s00449-018-2005-1</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)OLC2106647050</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-He213)s00449-018-2005-1-p</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">660</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">570</subfield><subfield code="a">690</subfield><subfield code="a">540</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">12</subfield><subfield code="2">ssgn</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Canales, Christian</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">The growth of Pichia pastoris $ Mut^{+} $ on methanol–glycerol mixtures fits to interactive dual-limited kinetics: model development and application to optimised fed-batch operation for heterologous protein production</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2018</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">ohne Hilfsmittel zu benutzen</subfield><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Band</subfield><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">© Springer-Verlag GmbH Germany, part of Springer Nature 2018</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract The methanol–glycerol co-feeding during the induction stage for heterologous protein production in Pichia pastoris has shown significant productive applications. Available model analysis applied to this dual-limited condition is scarce and normally does not consider the interaction effects between the substrates. In this work, a dual-limited growth model of P. pastoris considering an interactive kinetic effect was applied to an optimised fed-batch process production of heterologous Rhizopus oryzae lipase (ROL). In the proposed model, the growth kinetics on glycerol is fully expressed, whereas methanol kinetics is modulated by the co-metabolisation of glycerol, resulting in an enhancing effect of glycerol-specific growth rate. The modelling approach of fed-batch cultures also included the methanol volatilisation caused by the aeration that was found to be a not-negligible phenomenon. The model predicts the ability of P. pastoris to keep control of the methanol concentration in the broth during ROL-optimised production process in fed batch and fits satisfactorily the specific cell growth rate and ROL production. Implications of interaction effect are discussed applying the general procedure of modelling approach.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Dual-limited growth modelling</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Additive-enhancing kinetic model</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Heterologous protein production</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Methanol–glycerol mixed feeding</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Fed-batch exponential feeding</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Altamirano, Claudia</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Berrios, Julio</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Bioprocess and biosystems engineering</subfield><subfield code="d">Springer Berlin Heidelberg, 2001</subfield><subfield code="g">41(2018), 12 vom: 08. Sept., Seite 1827-1838</subfield><subfield code="w">(DE-627)333469763</subfield><subfield code="w">(DE-600)2056063-1</subfield><subfield code="w">(DE-576)094533709</subfield><subfield code="x">1615-7591</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:41</subfield><subfield code="g">year:2018</subfield><subfield code="g">number:12</subfield><subfield code="g">day:08</subfield><subfield code="g">month:09</subfield><subfield code="g">pages:1827-1838</subfield></datafield><datafield tag="856" ind1="4" ind2="1"><subfield code="u">https://doi.org/10.1007/s00449-018-2005-1</subfield><subfield code="z">lizenzpflichtig</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_OLC</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-UMW</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-ARC</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-TEC</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-CHE</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_24</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_31</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_70</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2018</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4277</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">41</subfield><subfield code="j">2018</subfield><subfield code="e">12</subfield><subfield code="b">08</subfield><subfield code="c">09</subfield><subfield code="h">1827-1838</subfield></datafield></record></collection>
|
| author |
Canales, Christian |
| spellingShingle |
Canales, Christian ddc 660 ddc 570 ssgn 12 misc Dual-limited growth modelling misc Additive-enhancing kinetic model misc Heterologous protein production misc Methanol–glycerol mixed feeding misc Fed-batch exponential feeding The growth of Pichia pastoris $ Mut^{+} $ on methanol–glycerol mixtures fits to interactive dual-limited kinetics: model development and application to optimised fed-batch operation for heterologous protein production |
| authorStr |
Canales, Christian |
| ppnlink_with_tag_str_mv |
@@773@@(DE-627)333469763 |
| format |
Article |
| dewey-ones |
660 - Chemical engineering 570 - Life sciences; biology 690 - Buildings 540 - Chemistry & allied sciences |
| delete_txt_mv |
keep |
| author_role |
aut aut aut |
| collection |
OLC |
| remote_str |
false |
| illustrated |
Not Illustrated |
| issn |
1615-7591 |
| topic_title |
660 VZ 570 690 540 VZ 12 ssgn The growth of Pichia pastoris $ Mut^{+} $ on methanol–glycerol mixtures fits to interactive dual-limited kinetics: model development and application to optimised fed-batch operation for heterologous protein production Dual-limited growth modelling Additive-enhancing kinetic model Heterologous protein production Methanol–glycerol mixed feeding Fed-batch exponential feeding |
| topic |
ddc 660 ddc 570 ssgn 12 misc Dual-limited growth modelling misc Additive-enhancing kinetic model misc Heterologous protein production misc Methanol–glycerol mixed feeding misc Fed-batch exponential feeding |
| topic_unstemmed |
ddc 660 ddc 570 ssgn 12 misc Dual-limited growth modelling misc Additive-enhancing kinetic model misc Heterologous protein production misc Methanol–glycerol mixed feeding misc Fed-batch exponential feeding |
| topic_browse |
ddc 660 ddc 570 ssgn 12 misc Dual-limited growth modelling misc Additive-enhancing kinetic model misc Heterologous protein production misc Methanol–glycerol mixed feeding misc Fed-batch exponential feeding |
| format_facet |
Aufsätze Gedruckte Aufsätze |
| format_main_str_mv |
Text Zeitschrift/Artikel |
| carriertype_str_mv |
nc |
| hierarchy_parent_title |
Bioprocess and biosystems engineering |
| hierarchy_parent_id |
333469763 |
| dewey-tens |
660 - Chemical engineering 570 - Life sciences; biology 690 - Building & construction 540 - Chemistry |
| hierarchy_top_title |
Bioprocess and biosystems engineering |
| isfreeaccess_txt |
false |
| familylinks_str_mv |
(DE-627)333469763 (DE-600)2056063-1 (DE-576)094533709 |
| title |
The growth of Pichia pastoris $ Mut^{+} $ on methanol–glycerol mixtures fits to interactive dual-limited kinetics: model development and application to optimised fed-batch operation for heterologous protein production |
| ctrlnum |
(DE-627)OLC2106647050 (DE-He213)s00449-018-2005-1-p |
| title_full |
The growth of Pichia pastoris $ Mut^{+} $ on methanol–glycerol mixtures fits to interactive dual-limited kinetics: model development and application to optimised fed-batch operation for heterologous protein production |
| author_sort |
Canales, Christian |
| journal |
Bioprocess and biosystems engineering |
| journalStr |
Bioprocess and biosystems engineering |
| lang_code |
eng |
| isOA_bool |
false |
| dewey-hundreds |
600 - Technology 500 - Science |
| recordtype |
marc |
| publishDateSort |
2018 |
| contenttype_str_mv |
txt |
| container_start_page |
1827 |
| author_browse |
Canales, Christian Altamirano, Claudia Berrios, Julio |
| container_volume |
41 |
| class |
660 VZ 570 690 540 VZ 12 ssgn |
| format_se |
Aufsätze |
| author-letter |
Canales, Christian |
| doi_str_mv |
10.1007/s00449-018-2005-1 |
| dewey-full |
660 570 690 540 |
| title_sort |
the growth of pichia pastoris $ mut^{+} $ on methanol–glycerol mixtures fits to interactive dual-limited kinetics: model development and application to optimised fed-batch operation for heterologous protein production |
| title_auth |
The growth of Pichia pastoris $ Mut^{+} $ on methanol–glycerol mixtures fits to interactive dual-limited kinetics: model development and application to optimised fed-batch operation for heterologous protein production |
| abstract |
Abstract The methanol–glycerol co-feeding during the induction stage for heterologous protein production in Pichia pastoris has shown significant productive applications. Available model analysis applied to this dual-limited condition is scarce and normally does not consider the interaction effects between the substrates. In this work, a dual-limited growth model of P. pastoris considering an interactive kinetic effect was applied to an optimised fed-batch process production of heterologous Rhizopus oryzae lipase (ROL). In the proposed model, the growth kinetics on glycerol is fully expressed, whereas methanol kinetics is modulated by the co-metabolisation of glycerol, resulting in an enhancing effect of glycerol-specific growth rate. The modelling approach of fed-batch cultures also included the methanol volatilisation caused by the aeration that was found to be a not-negligible phenomenon. The model predicts the ability of P. pastoris to keep control of the methanol concentration in the broth during ROL-optimised production process in fed batch and fits satisfactorily the specific cell growth rate and ROL production. Implications of interaction effect are discussed applying the general procedure of modelling approach. © Springer-Verlag GmbH Germany, part of Springer Nature 2018 |
| abstractGer |
Abstract The methanol–glycerol co-feeding during the induction stage for heterologous protein production in Pichia pastoris has shown significant productive applications. Available model analysis applied to this dual-limited condition is scarce and normally does not consider the interaction effects between the substrates. In this work, a dual-limited growth model of P. pastoris considering an interactive kinetic effect was applied to an optimised fed-batch process production of heterologous Rhizopus oryzae lipase (ROL). In the proposed model, the growth kinetics on glycerol is fully expressed, whereas methanol kinetics is modulated by the co-metabolisation of glycerol, resulting in an enhancing effect of glycerol-specific growth rate. The modelling approach of fed-batch cultures also included the methanol volatilisation caused by the aeration that was found to be a not-negligible phenomenon. The model predicts the ability of P. pastoris to keep control of the methanol concentration in the broth during ROL-optimised production process in fed batch and fits satisfactorily the specific cell growth rate and ROL production. Implications of interaction effect are discussed applying the general procedure of modelling approach. © Springer-Verlag GmbH Germany, part of Springer Nature 2018 |
| abstract_unstemmed |
Abstract The methanol–glycerol co-feeding during the induction stage for heterologous protein production in Pichia pastoris has shown significant productive applications. Available model analysis applied to this dual-limited condition is scarce and normally does not consider the interaction effects between the substrates. In this work, a dual-limited growth model of P. pastoris considering an interactive kinetic effect was applied to an optimised fed-batch process production of heterologous Rhizopus oryzae lipase (ROL). In the proposed model, the growth kinetics on glycerol is fully expressed, whereas methanol kinetics is modulated by the co-metabolisation of glycerol, resulting in an enhancing effect of glycerol-specific growth rate. The modelling approach of fed-batch cultures also included the methanol volatilisation caused by the aeration that was found to be a not-negligible phenomenon. The model predicts the ability of P. pastoris to keep control of the methanol concentration in the broth during ROL-optimised production process in fed batch and fits satisfactorily the specific cell growth rate and ROL production. Implications of interaction effect are discussed applying the general procedure of modelling approach. © Springer-Verlag GmbH Germany, part of Springer Nature 2018 |
| collection_details |
GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-ARC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_24 GBV_ILN_31 GBV_ILN_70 GBV_ILN_2018 GBV_ILN_4277 |
| container_issue |
12 |
| title_short |
The growth of Pichia pastoris $ Mut^{+} $ on methanol–glycerol mixtures fits to interactive dual-limited kinetics: model development and application to optimised fed-batch operation for heterologous protein production |
| url |
https://doi.org/10.1007/s00449-018-2005-1 |
| remote_bool |
false |
| author2 |
Altamirano, Claudia Berrios, Julio |
| author2Str |
Altamirano, Claudia Berrios, Julio |
| ppnlink |
333469763 |
| mediatype_str_mv |
n |
| isOA_txt |
false |
| hochschulschrift_bool |
false |
| doi_str |
10.1007/s00449-018-2005-1 |
| up_date |
2024-07-04T07:12:39.822Z |
| _version_ |
1803631627930173440 |
| fullrecord_marcxml |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000naa a22002652 4500</leader><controlfield tag="001">OLC2106647050</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230403073534.0</controlfield><controlfield tag="007">tu</controlfield><controlfield tag="008">230403s2018 xx ||||| 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/s00449-018-2005-1</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)OLC2106647050</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-He213)s00449-018-2005-1-p</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">660</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">570</subfield><subfield code="a">690</subfield><subfield code="a">540</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">12</subfield><subfield code="2">ssgn</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Canales, Christian</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">The growth of Pichia pastoris $ Mut^{+} $ on methanol–glycerol mixtures fits to interactive dual-limited kinetics: model development and application to optimised fed-batch operation for heterologous protein production</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2018</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">ohne Hilfsmittel zu benutzen</subfield><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Band</subfield><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">© Springer-Verlag GmbH Germany, part of Springer Nature 2018</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract The methanol–glycerol co-feeding during the induction stage for heterologous protein production in Pichia pastoris has shown significant productive applications. Available model analysis applied to this dual-limited condition is scarce and normally does not consider the interaction effects between the substrates. In this work, a dual-limited growth model of P. pastoris considering an interactive kinetic effect was applied to an optimised fed-batch process production of heterologous Rhizopus oryzae lipase (ROL). In the proposed model, the growth kinetics on glycerol is fully expressed, whereas methanol kinetics is modulated by the co-metabolisation of glycerol, resulting in an enhancing effect of glycerol-specific growth rate. The modelling approach of fed-batch cultures also included the methanol volatilisation caused by the aeration that was found to be a not-negligible phenomenon. The model predicts the ability of P. pastoris to keep control of the methanol concentration in the broth during ROL-optimised production process in fed batch and fits satisfactorily the specific cell growth rate and ROL production. Implications of interaction effect are discussed applying the general procedure of modelling approach.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Dual-limited growth modelling</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Additive-enhancing kinetic model</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Heterologous protein production</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Methanol–glycerol mixed feeding</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Fed-batch exponential feeding</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Altamirano, Claudia</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Berrios, Julio</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Bioprocess and biosystems engineering</subfield><subfield code="d">Springer Berlin Heidelberg, 2001</subfield><subfield code="g">41(2018), 12 vom: 08. Sept., Seite 1827-1838</subfield><subfield code="w">(DE-627)333469763</subfield><subfield code="w">(DE-600)2056063-1</subfield><subfield code="w">(DE-576)094533709</subfield><subfield code="x">1615-7591</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:41</subfield><subfield code="g">year:2018</subfield><subfield code="g">number:12</subfield><subfield code="g">day:08</subfield><subfield code="g">month:09</subfield><subfield code="g">pages:1827-1838</subfield></datafield><datafield tag="856" ind1="4" ind2="1"><subfield code="u">https://doi.org/10.1007/s00449-018-2005-1</subfield><subfield code="z">lizenzpflichtig</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_OLC</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-UMW</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-ARC</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-TEC</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-CHE</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_24</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_31</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_70</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2018</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4277</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">41</subfield><subfield code="j">2018</subfield><subfield code="e">12</subfield><subfield code="b">08</subfield><subfield code="c">09</subfield><subfield code="h">1827-1838</subfield></datafield></record></collection>
|
| score |
7.3976707 |