Cantilever Nanobiosensor Functionalized with Tyrosinase for Detection of Estrone and β-estradiol in Water
Abstract This work aimed to develop cantilever nanobiosensor functionalized with tyrosinase enzyme to detect 17β-estradiol and estrone hormones. In this system, the tyrosinase enzyme was covalently immobilized by self-assembled monolayer onto the cantilever sensor surface. It was possible to verify...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
de Cezaro, Alana Marie [verfasserIn] |
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| Format: |
Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2019 |
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| Schlagwörter: |
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| Anmerkung: |
© Springer Science+Business Media, LLC, part of Springer Nature 2019 |
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| Übergeordnetes Werk: |
Enthalten in: Applied biochemistry and biotechnology / A - Springer US, 1994, 190(2019), 4 vom: 29. Nov., Seite 1512-1524 |
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| Übergeordnetes Werk: |
volume:190 ; year:2019 ; number:4 ; day:29 ; month:11 ; pages:1512-1524 |
| Links: |
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| DOI / URN: |
10.1007/s12010-019-03195-8 |
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| Katalog-ID: |
OLC2117587216 |
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| 520 | |a Abstract This work aimed to develop cantilever nanobiosensor functionalized with tyrosinase enzyme to detect 17β-estradiol and estrone hormones. In this system, the tyrosinase enzyme was covalently immobilized by self-assembled monolayer onto the cantilever sensor surface. It was possible to verify that the high hormone concentration investigated resulted in high voltage response. The nanobiosensor presented a distinction between the concentrations evaluated and was verified sensitivities of 0.497 and 0.101 V/μg, limit of detection of 0.1 and 0.4 ng/L for the hormones 17β-estradiol and estrone, respectively. The device showed good reversibility and during 30 days of storage maintained about 99% of the original signal. The cantilever nanobiosensor applied in different water samples (ultrapure, river, tap, and mineral) showed good performance, so could be readily extended toward the on-site monitoring of the other trace small molecular pollutants in environmental water matrices. | ||
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10.1007/s12010-019-03195-8 doi (DE-627)OLC2117587216 (DE-He213)s12010-019-03195-8-p DE-627 ger DE-627 rakwb eng 570 540 660 VZ 12 ssgn 42.00 bkl de Cezaro, Alana Marie verfasserin (orcid)0000-0002-4654-5124 aut Cantilever Nanobiosensor Functionalized with Tyrosinase for Detection of Estrone and β-estradiol in Water 2019 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer Science+Business Media, LLC, part of Springer Nature 2019 Abstract This work aimed to develop cantilever nanobiosensor functionalized with tyrosinase enzyme to detect 17β-estradiol and estrone hormones. In this system, the tyrosinase enzyme was covalently immobilized by self-assembled monolayer onto the cantilever sensor surface. It was possible to verify that the high hormone concentration investigated resulted in high voltage response. The nanobiosensor presented a distinction between the concentrations evaluated and was verified sensitivities of 0.497 and 0.101 V/μg, limit of detection of 0.1 and 0.4 ng/L for the hormones 17β-estradiol and estrone, respectively. The device showed good reversibility and during 30 days of storage maintained about 99% of the original signal. The cantilever nanobiosensor applied in different water samples (ultrapure, river, tap, and mineral) showed good performance, so could be readily extended toward the on-site monitoring of the other trace small molecular pollutants in environmental water matrices. Water Contaminants Hormones Enzyme Sensitivity Limit of detection Rigo, Aline Andressa (orcid)0000-0002-3198-1847 aut Martinazzo, Janine (orcid)0000-0003-1127-5555 aut Muenchen, Daniela Kunkel (orcid)0000-0003-1392-8425 aut Manzoli, Alexandra (orcid)0000-0003-4148-6337 aut Correa, Daniel Souza (orcid)0000-0002-5592-0627 aut Steffens, Juliana (orcid)0000-0001-6607-2283 aut Steffens, Clarice (orcid)0000-0003-4394-125X aut Enthalten in Applied biochemistry and biotechnology / A Springer US, 1994 190(2019), 4 vom: 29. Nov., Seite 1512-1524 (DE-627)182278573 (DE-600)1193054-8 (DE-576)043085105 0273-2289 nnns volume:190 year:2019 number:4 day:29 month:11 pages:1512-1524 https://doi.org/10.1007/s12010-019-03195-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_70 42.00 VZ AR 190 2019 4 29 11 1512-1524 |
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10.1007/s12010-019-03195-8 doi (DE-627)OLC2117587216 (DE-He213)s12010-019-03195-8-p DE-627 ger DE-627 rakwb eng 570 540 660 VZ 12 ssgn 42.00 bkl de Cezaro, Alana Marie verfasserin (orcid)0000-0002-4654-5124 aut Cantilever Nanobiosensor Functionalized with Tyrosinase for Detection of Estrone and β-estradiol in Water 2019 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer Science+Business Media, LLC, part of Springer Nature 2019 Abstract This work aimed to develop cantilever nanobiosensor functionalized with tyrosinase enzyme to detect 17β-estradiol and estrone hormones. In this system, the tyrosinase enzyme was covalently immobilized by self-assembled monolayer onto the cantilever sensor surface. It was possible to verify that the high hormone concentration investigated resulted in high voltage response. The nanobiosensor presented a distinction between the concentrations evaluated and was verified sensitivities of 0.497 and 0.101 V/μg, limit of detection of 0.1 and 0.4 ng/L for the hormones 17β-estradiol and estrone, respectively. The device showed good reversibility and during 30 days of storage maintained about 99% of the original signal. The cantilever nanobiosensor applied in different water samples (ultrapure, river, tap, and mineral) showed good performance, so could be readily extended toward the on-site monitoring of the other trace small molecular pollutants in environmental water matrices. Water Contaminants Hormones Enzyme Sensitivity Limit of detection Rigo, Aline Andressa (orcid)0000-0002-3198-1847 aut Martinazzo, Janine (orcid)0000-0003-1127-5555 aut Muenchen, Daniela Kunkel (orcid)0000-0003-1392-8425 aut Manzoli, Alexandra (orcid)0000-0003-4148-6337 aut Correa, Daniel Souza (orcid)0000-0002-5592-0627 aut Steffens, Juliana (orcid)0000-0001-6607-2283 aut Steffens, Clarice (orcid)0000-0003-4394-125X aut Enthalten in Applied biochemistry and biotechnology / A Springer US, 1994 190(2019), 4 vom: 29. Nov., Seite 1512-1524 (DE-627)182278573 (DE-600)1193054-8 (DE-576)043085105 0273-2289 nnns volume:190 year:2019 number:4 day:29 month:11 pages:1512-1524 https://doi.org/10.1007/s12010-019-03195-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_70 42.00 VZ AR 190 2019 4 29 11 1512-1524 |
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10.1007/s12010-019-03195-8 doi (DE-627)OLC2117587216 (DE-He213)s12010-019-03195-8-p DE-627 ger DE-627 rakwb eng 570 540 660 VZ 12 ssgn 42.00 bkl de Cezaro, Alana Marie verfasserin (orcid)0000-0002-4654-5124 aut Cantilever Nanobiosensor Functionalized with Tyrosinase for Detection of Estrone and β-estradiol in Water 2019 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer Science+Business Media, LLC, part of Springer Nature 2019 Abstract This work aimed to develop cantilever nanobiosensor functionalized with tyrosinase enzyme to detect 17β-estradiol and estrone hormones. In this system, the tyrosinase enzyme was covalently immobilized by self-assembled monolayer onto the cantilever sensor surface. It was possible to verify that the high hormone concentration investigated resulted in high voltage response. The nanobiosensor presented a distinction between the concentrations evaluated and was verified sensitivities of 0.497 and 0.101 V/μg, limit of detection of 0.1 and 0.4 ng/L for the hormones 17β-estradiol and estrone, respectively. The device showed good reversibility and during 30 days of storage maintained about 99% of the original signal. The cantilever nanobiosensor applied in different water samples (ultrapure, river, tap, and mineral) showed good performance, so could be readily extended toward the on-site monitoring of the other trace small molecular pollutants in environmental water matrices. Water Contaminants Hormones Enzyme Sensitivity Limit of detection Rigo, Aline Andressa (orcid)0000-0002-3198-1847 aut Martinazzo, Janine (orcid)0000-0003-1127-5555 aut Muenchen, Daniela Kunkel (orcid)0000-0003-1392-8425 aut Manzoli, Alexandra (orcid)0000-0003-4148-6337 aut Correa, Daniel Souza (orcid)0000-0002-5592-0627 aut Steffens, Juliana (orcid)0000-0001-6607-2283 aut Steffens, Clarice (orcid)0000-0003-4394-125X aut Enthalten in Applied biochemistry and biotechnology / A Springer US, 1994 190(2019), 4 vom: 29. Nov., Seite 1512-1524 (DE-627)182278573 (DE-600)1193054-8 (DE-576)043085105 0273-2289 nnns volume:190 year:2019 number:4 day:29 month:11 pages:1512-1524 https://doi.org/10.1007/s12010-019-03195-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_70 42.00 VZ AR 190 2019 4 29 11 1512-1524 |
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10.1007/s12010-019-03195-8 doi (DE-627)OLC2117587216 (DE-He213)s12010-019-03195-8-p DE-627 ger DE-627 rakwb eng 570 540 660 VZ 12 ssgn 42.00 bkl de Cezaro, Alana Marie verfasserin (orcid)0000-0002-4654-5124 aut Cantilever Nanobiosensor Functionalized with Tyrosinase for Detection of Estrone and β-estradiol in Water 2019 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer Science+Business Media, LLC, part of Springer Nature 2019 Abstract This work aimed to develop cantilever nanobiosensor functionalized with tyrosinase enzyme to detect 17β-estradiol and estrone hormones. In this system, the tyrosinase enzyme was covalently immobilized by self-assembled monolayer onto the cantilever sensor surface. It was possible to verify that the high hormone concentration investigated resulted in high voltage response. The nanobiosensor presented a distinction between the concentrations evaluated and was verified sensitivities of 0.497 and 0.101 V/μg, limit of detection of 0.1 and 0.4 ng/L for the hormones 17β-estradiol and estrone, respectively. The device showed good reversibility and during 30 days of storage maintained about 99% of the original signal. The cantilever nanobiosensor applied in different water samples (ultrapure, river, tap, and mineral) showed good performance, so could be readily extended toward the on-site monitoring of the other trace small molecular pollutants in environmental water matrices. Water Contaminants Hormones Enzyme Sensitivity Limit of detection Rigo, Aline Andressa (orcid)0000-0002-3198-1847 aut Martinazzo, Janine (orcid)0000-0003-1127-5555 aut Muenchen, Daniela Kunkel (orcid)0000-0003-1392-8425 aut Manzoli, Alexandra (orcid)0000-0003-4148-6337 aut Correa, Daniel Souza (orcid)0000-0002-5592-0627 aut Steffens, Juliana (orcid)0000-0001-6607-2283 aut Steffens, Clarice (orcid)0000-0003-4394-125X aut Enthalten in Applied biochemistry and biotechnology / A Springer US, 1994 190(2019), 4 vom: 29. Nov., Seite 1512-1524 (DE-627)182278573 (DE-600)1193054-8 (DE-576)043085105 0273-2289 nnns volume:190 year:2019 number:4 day:29 month:11 pages:1512-1524 https://doi.org/10.1007/s12010-019-03195-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_70 42.00 VZ AR 190 2019 4 29 11 1512-1524 |
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10.1007/s12010-019-03195-8 doi (DE-627)OLC2117587216 (DE-He213)s12010-019-03195-8-p DE-627 ger DE-627 rakwb eng 570 540 660 VZ 12 ssgn 42.00 bkl de Cezaro, Alana Marie verfasserin (orcid)0000-0002-4654-5124 aut Cantilever Nanobiosensor Functionalized with Tyrosinase for Detection of Estrone and β-estradiol in Water 2019 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer Science+Business Media, LLC, part of Springer Nature 2019 Abstract This work aimed to develop cantilever nanobiosensor functionalized with tyrosinase enzyme to detect 17β-estradiol and estrone hormones. In this system, the tyrosinase enzyme was covalently immobilized by self-assembled monolayer onto the cantilever sensor surface. It was possible to verify that the high hormone concentration investigated resulted in high voltage response. The nanobiosensor presented a distinction between the concentrations evaluated and was verified sensitivities of 0.497 and 0.101 V/μg, limit of detection of 0.1 and 0.4 ng/L for the hormones 17β-estradiol and estrone, respectively. The device showed good reversibility and during 30 days of storage maintained about 99% of the original signal. The cantilever nanobiosensor applied in different water samples (ultrapure, river, tap, and mineral) showed good performance, so could be readily extended toward the on-site monitoring of the other trace small molecular pollutants in environmental water matrices. Water Contaminants Hormones Enzyme Sensitivity Limit of detection Rigo, Aline Andressa (orcid)0000-0002-3198-1847 aut Martinazzo, Janine (orcid)0000-0003-1127-5555 aut Muenchen, Daniela Kunkel (orcid)0000-0003-1392-8425 aut Manzoli, Alexandra (orcid)0000-0003-4148-6337 aut Correa, Daniel Souza (orcid)0000-0002-5592-0627 aut Steffens, Juliana (orcid)0000-0001-6607-2283 aut Steffens, Clarice (orcid)0000-0003-4394-125X aut Enthalten in Applied biochemistry and biotechnology / A Springer US, 1994 190(2019), 4 vom: 29. Nov., Seite 1512-1524 (DE-627)182278573 (DE-600)1193054-8 (DE-576)043085105 0273-2289 nnns volume:190 year:2019 number:4 day:29 month:11 pages:1512-1524 https://doi.org/10.1007/s12010-019-03195-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_70 42.00 VZ AR 190 2019 4 29 11 1512-1524 |
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de Cezaro, Alana Marie @@aut@@ Rigo, Aline Andressa @@aut@@ Martinazzo, Janine @@aut@@ Muenchen, Daniela Kunkel @@aut@@ Manzoli, Alexandra @@aut@@ Correa, Daniel Souza @@aut@@ Steffens, Juliana @@aut@@ Steffens, Clarice @@aut@@ |
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Cantilever Nanobiosensor Functionalized with Tyrosinase for Detection of Estrone and β-estradiol in Water |
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Abstract This work aimed to develop cantilever nanobiosensor functionalized with tyrosinase enzyme to detect 17β-estradiol and estrone hormones. In this system, the tyrosinase enzyme was covalently immobilized by self-assembled monolayer onto the cantilever sensor surface. It was possible to verify that the high hormone concentration investigated resulted in high voltage response. The nanobiosensor presented a distinction between the concentrations evaluated and was verified sensitivities of 0.497 and 0.101 V/μg, limit of detection of 0.1 and 0.4 ng/L for the hormones 17β-estradiol and estrone, respectively. The device showed good reversibility and during 30 days of storage maintained about 99% of the original signal. The cantilever nanobiosensor applied in different water samples (ultrapure, river, tap, and mineral) showed good performance, so could be readily extended toward the on-site monitoring of the other trace small molecular pollutants in environmental water matrices. © Springer Science+Business Media, LLC, part of Springer Nature 2019 |
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Abstract This work aimed to develop cantilever nanobiosensor functionalized with tyrosinase enzyme to detect 17β-estradiol and estrone hormones. In this system, the tyrosinase enzyme was covalently immobilized by self-assembled monolayer onto the cantilever sensor surface. It was possible to verify that the high hormone concentration investigated resulted in high voltage response. The nanobiosensor presented a distinction between the concentrations evaluated and was verified sensitivities of 0.497 and 0.101 V/μg, limit of detection of 0.1 and 0.4 ng/L for the hormones 17β-estradiol and estrone, respectively. The device showed good reversibility and during 30 days of storage maintained about 99% of the original signal. The cantilever nanobiosensor applied in different water samples (ultrapure, river, tap, and mineral) showed good performance, so could be readily extended toward the on-site monitoring of the other trace small molecular pollutants in environmental water matrices. © Springer Science+Business Media, LLC, part of Springer Nature 2019 |
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Abstract This work aimed to develop cantilever nanobiosensor functionalized with tyrosinase enzyme to detect 17β-estradiol and estrone hormones. In this system, the tyrosinase enzyme was covalently immobilized by self-assembled monolayer onto the cantilever sensor surface. It was possible to verify that the high hormone concentration investigated resulted in high voltage response. The nanobiosensor presented a distinction between the concentrations evaluated and was verified sensitivities of 0.497 and 0.101 V/μg, limit of detection of 0.1 and 0.4 ng/L for the hormones 17β-estradiol and estrone, respectively. The device showed good reversibility and during 30 days of storage maintained about 99% of the original signal. The cantilever nanobiosensor applied in different water samples (ultrapure, river, tap, and mineral) showed good performance, so could be readily extended toward the on-site monitoring of the other trace small molecular pollutants in environmental water matrices. © Springer Science+Business Media, LLC, part of Springer Nature 2019 |
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