Towards upscaling the valorization of wheat straw residues: alkaline pretreatment using sodium hydroxide, enzymatic hydrolysis and biogas production
Abstract Lignocellulosic biomass is considered as a recalcitrant substrate for anaerobic digestion due to its complex nature that limits its biological degradation. Therefore, suitable preprocessing for the improvement of the performance of conventional anaerobic digestion remains a challenge in the...
Ausführliche Beschreibung
Autor*in: |
Novakovic, Jelica [verfasserIn] |
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Artikel |
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Sprache: |
Englisch |
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2020 |
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Anmerkung: |
© Springer-Verlag GmbH Germany, part of Springer Nature 2020 |
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Übergeordnetes Werk: |
Enthalten in: Environmental science and pollution research - Springer Berlin Heidelberg, 1994, 28(2020), 19 vom: 28. Apr., Seite 24486-24498 |
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Übergeordnetes Werk: |
volume:28 ; year:2020 ; number:19 ; day:28 ; month:04 ; pages:24486-24498 |
Links: |
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DOI / URN: |
10.1007/s11356-020-08905-y |
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Katalog-ID: |
OLC2125671352 |
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520 | |a Abstract Lignocellulosic biomass is considered as a recalcitrant substrate for anaerobic digestion due to its complex nature that limits its biological degradation. Therefore, suitable preprocessing for the improvement of the performance of conventional anaerobic digestion remains a challenge in the development of anaerobic digestion technology. The physical and chemical characteristics of wheat straw (WS), as a representative lignocellulosic biomass, have a significant impact on the anaerobic digestion process in terms of quantity and quality of the produced biogas. This study aimed at investigating the enzymatic saccharification and detoxification of straw prior to anaerobic digestion with the final objective of enhancing the performance of conventional anaerobic systems of recalcitrant fractions of agricultural waste. The experimental activity was performed in lab and pilot scale treating WS. Alkaline delignification of straw using sodium hydroxide (NaOH) was studied prior to enzymatic hydrolysis for the production of easily biodegradable sugars. After defining the optimum conditions for the pretreatment scheme, the anaerobic digestability of the effluents produced was measured. Finally, the final liquid effluents were fed to a pilot scale anaerobic digester of 0.5 $ m^{3} $ volume, applying an increasing organic loading rate (OLR) regime (in terms of chemical oxygen demand (COD) from 0.2 to 15 kg COD/$ m^{3} $/day). The optimum conditions for the delignification and enzymatic hydrolysis of WS were defined as 0.5 M NaOH at 50 °C for 3–5 h and 15 μL Cellic CTec2/g pretreated straw at 50 °C. It was proven that the resulting liquid effluents could be fed to an anaerobic digester in the ratio that they are produced with satisfactory COD removal efficiencies (over 70%) for OLRs up to 10 kg COD/$ m^{3} $/day. This value is correspondent to a hydraulic retention time of around 7.5 days, much lower than the respective one for untreated straw (over 12 days). | ||
650 | 4 | |a Anaerobic digestion | |
650 | 4 | |a Delignification | |
650 | 4 | |a Enzyme loading | |
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700 | 1 | |a Moustakas, Konstantinos |4 aut | |
700 | 1 | |a Malamis, Dimitris |4 aut | |
700 | 1 | |a Loizidou, Maria |4 aut | |
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10.1007/s11356-020-08905-y doi (DE-627)OLC2125671352 (DE-He213)s11356-020-08905-y-p DE-627 ger DE-627 rakwb eng 570 360 333.7 VZ 690 333.7 540 VZ BIODIV DE-30 fid Novakovic, Jelica verfasserin aut Towards upscaling the valorization of wheat straw residues: alkaline pretreatment using sodium hydroxide, enzymatic hydrolysis and biogas production 2020 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag GmbH Germany, part of Springer Nature 2020 Abstract Lignocellulosic biomass is considered as a recalcitrant substrate for anaerobic digestion due to its complex nature that limits its biological degradation. Therefore, suitable preprocessing for the improvement of the performance of conventional anaerobic digestion remains a challenge in the development of anaerobic digestion technology. The physical and chemical characteristics of wheat straw (WS), as a representative lignocellulosic biomass, have a significant impact on the anaerobic digestion process in terms of quantity and quality of the produced biogas. This study aimed at investigating the enzymatic saccharification and detoxification of straw prior to anaerobic digestion with the final objective of enhancing the performance of conventional anaerobic systems of recalcitrant fractions of agricultural waste. The experimental activity was performed in lab and pilot scale treating WS. Alkaline delignification of straw using sodium hydroxide (NaOH) was studied prior to enzymatic hydrolysis for the production of easily biodegradable sugars. After defining the optimum conditions for the pretreatment scheme, the anaerobic digestability of the effluents produced was measured. Finally, the final liquid effluents were fed to a pilot scale anaerobic digester of 0.5 $ m^{3} $ volume, applying an increasing organic loading rate (OLR) regime (in terms of chemical oxygen demand (COD) from 0.2 to 15 kg COD/$ m^{3} $/day). The optimum conditions for the delignification and enzymatic hydrolysis of WS were defined as 0.5 M NaOH at 50 °C for 3–5 h and 15 μL Cellic CTec2/g pretreated straw at 50 °C. It was proven that the resulting liquid effluents could be fed to an anaerobic digester in the ratio that they are produced with satisfactory COD removal efficiencies (over 70%) for OLRs up to 10 kg COD/$ m^{3} $/day. This value is correspondent to a hydraulic retention time of around 7.5 days, much lower than the respective one for untreated straw (over 12 days). Anaerobic digestion Delignification Enzyme loading Particle size Scale-up Sugars yield Kontogianni, Nikoleta aut Barampouti, Elli Maria aut Mai, Sofia aut Moustakas, Konstantinos aut Malamis, Dimitris aut Loizidou, Maria aut Enthalten in Environmental science and pollution research Springer Berlin Heidelberg, 1994 28(2020), 19 vom: 28. Apr., Seite 24486-24498 (DE-627)171335805 (DE-600)1178791-0 (DE-576)038875101 0944-1344 nnns volume:28 year:2020 number:19 day:28 month:04 pages:24486-24498 https://doi.org/10.1007/s11356-020-08905-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-UMW SSG-OLC-ARC SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-FOR GBV_ILN_252 GBV_ILN_267 GBV_ILN_2018 GBV_ILN_4277 AR 28 2020 19 28 04 24486-24498 |
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10.1007/s11356-020-08905-y doi (DE-627)OLC2125671352 (DE-He213)s11356-020-08905-y-p DE-627 ger DE-627 rakwb eng 570 360 333.7 VZ 690 333.7 540 VZ BIODIV DE-30 fid Novakovic, Jelica verfasserin aut Towards upscaling the valorization of wheat straw residues: alkaline pretreatment using sodium hydroxide, enzymatic hydrolysis and biogas production 2020 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag GmbH Germany, part of Springer Nature 2020 Abstract Lignocellulosic biomass is considered as a recalcitrant substrate for anaerobic digestion due to its complex nature that limits its biological degradation. Therefore, suitable preprocessing for the improvement of the performance of conventional anaerobic digestion remains a challenge in the development of anaerobic digestion technology. The physical and chemical characteristics of wheat straw (WS), as a representative lignocellulosic biomass, have a significant impact on the anaerobic digestion process in terms of quantity and quality of the produced biogas. This study aimed at investigating the enzymatic saccharification and detoxification of straw prior to anaerobic digestion with the final objective of enhancing the performance of conventional anaerobic systems of recalcitrant fractions of agricultural waste. The experimental activity was performed in lab and pilot scale treating WS. Alkaline delignification of straw using sodium hydroxide (NaOH) was studied prior to enzymatic hydrolysis for the production of easily biodegradable sugars. After defining the optimum conditions for the pretreatment scheme, the anaerobic digestability of the effluents produced was measured. Finally, the final liquid effluents were fed to a pilot scale anaerobic digester of 0.5 $ m^{3} $ volume, applying an increasing organic loading rate (OLR) regime (in terms of chemical oxygen demand (COD) from 0.2 to 15 kg COD/$ m^{3} $/day). The optimum conditions for the delignification and enzymatic hydrolysis of WS were defined as 0.5 M NaOH at 50 °C for 3–5 h and 15 μL Cellic CTec2/g pretreated straw at 50 °C. It was proven that the resulting liquid effluents could be fed to an anaerobic digester in the ratio that they are produced with satisfactory COD removal efficiencies (over 70%) for OLRs up to 10 kg COD/$ m^{3} $/day. This value is correspondent to a hydraulic retention time of around 7.5 days, much lower than the respective one for untreated straw (over 12 days). Anaerobic digestion Delignification Enzyme loading Particle size Scale-up Sugars yield Kontogianni, Nikoleta aut Barampouti, Elli Maria aut Mai, Sofia aut Moustakas, Konstantinos aut Malamis, Dimitris aut Loizidou, Maria aut Enthalten in Environmental science and pollution research Springer Berlin Heidelberg, 1994 28(2020), 19 vom: 28. Apr., Seite 24486-24498 (DE-627)171335805 (DE-600)1178791-0 (DE-576)038875101 0944-1344 nnns volume:28 year:2020 number:19 day:28 month:04 pages:24486-24498 https://doi.org/10.1007/s11356-020-08905-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-UMW SSG-OLC-ARC SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-FOR GBV_ILN_252 GBV_ILN_267 GBV_ILN_2018 GBV_ILN_4277 AR 28 2020 19 28 04 24486-24498 |
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10.1007/s11356-020-08905-y doi (DE-627)OLC2125671352 (DE-He213)s11356-020-08905-y-p DE-627 ger DE-627 rakwb eng 570 360 333.7 VZ 690 333.7 540 VZ BIODIV DE-30 fid Novakovic, Jelica verfasserin aut Towards upscaling the valorization of wheat straw residues: alkaline pretreatment using sodium hydroxide, enzymatic hydrolysis and biogas production 2020 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag GmbH Germany, part of Springer Nature 2020 Abstract Lignocellulosic biomass is considered as a recalcitrant substrate for anaerobic digestion due to its complex nature that limits its biological degradation. Therefore, suitable preprocessing for the improvement of the performance of conventional anaerobic digestion remains a challenge in the development of anaerobic digestion technology. The physical and chemical characteristics of wheat straw (WS), as a representative lignocellulosic biomass, have a significant impact on the anaerobic digestion process in terms of quantity and quality of the produced biogas. This study aimed at investigating the enzymatic saccharification and detoxification of straw prior to anaerobic digestion with the final objective of enhancing the performance of conventional anaerobic systems of recalcitrant fractions of agricultural waste. The experimental activity was performed in lab and pilot scale treating WS. Alkaline delignification of straw using sodium hydroxide (NaOH) was studied prior to enzymatic hydrolysis for the production of easily biodegradable sugars. After defining the optimum conditions for the pretreatment scheme, the anaerobic digestability of the effluents produced was measured. Finally, the final liquid effluents were fed to a pilot scale anaerobic digester of 0.5 $ m^{3} $ volume, applying an increasing organic loading rate (OLR) regime (in terms of chemical oxygen demand (COD) from 0.2 to 15 kg COD/$ m^{3} $/day). The optimum conditions for the delignification and enzymatic hydrolysis of WS were defined as 0.5 M NaOH at 50 °C for 3–5 h and 15 μL Cellic CTec2/g pretreated straw at 50 °C. It was proven that the resulting liquid effluents could be fed to an anaerobic digester in the ratio that they are produced with satisfactory COD removal efficiencies (over 70%) for OLRs up to 10 kg COD/$ m^{3} $/day. This value is correspondent to a hydraulic retention time of around 7.5 days, much lower than the respective one for untreated straw (over 12 days). Anaerobic digestion Delignification Enzyme loading Particle size Scale-up Sugars yield Kontogianni, Nikoleta aut Barampouti, Elli Maria aut Mai, Sofia aut Moustakas, Konstantinos aut Malamis, Dimitris aut Loizidou, Maria aut Enthalten in Environmental science and pollution research Springer Berlin Heidelberg, 1994 28(2020), 19 vom: 28. Apr., Seite 24486-24498 (DE-627)171335805 (DE-600)1178791-0 (DE-576)038875101 0944-1344 nnns volume:28 year:2020 number:19 day:28 month:04 pages:24486-24498 https://doi.org/10.1007/s11356-020-08905-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-UMW SSG-OLC-ARC SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-FOR GBV_ILN_252 GBV_ILN_267 GBV_ILN_2018 GBV_ILN_4277 AR 28 2020 19 28 04 24486-24498 |
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10.1007/s11356-020-08905-y doi (DE-627)OLC2125671352 (DE-He213)s11356-020-08905-y-p DE-627 ger DE-627 rakwb eng 570 360 333.7 VZ 690 333.7 540 VZ BIODIV DE-30 fid Novakovic, Jelica verfasserin aut Towards upscaling the valorization of wheat straw residues: alkaline pretreatment using sodium hydroxide, enzymatic hydrolysis and biogas production 2020 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag GmbH Germany, part of Springer Nature 2020 Abstract Lignocellulosic biomass is considered as a recalcitrant substrate for anaerobic digestion due to its complex nature that limits its biological degradation. Therefore, suitable preprocessing for the improvement of the performance of conventional anaerobic digestion remains a challenge in the development of anaerobic digestion technology. The physical and chemical characteristics of wheat straw (WS), as a representative lignocellulosic biomass, have a significant impact on the anaerobic digestion process in terms of quantity and quality of the produced biogas. This study aimed at investigating the enzymatic saccharification and detoxification of straw prior to anaerobic digestion with the final objective of enhancing the performance of conventional anaerobic systems of recalcitrant fractions of agricultural waste. The experimental activity was performed in lab and pilot scale treating WS. Alkaline delignification of straw using sodium hydroxide (NaOH) was studied prior to enzymatic hydrolysis for the production of easily biodegradable sugars. After defining the optimum conditions for the pretreatment scheme, the anaerobic digestability of the effluents produced was measured. Finally, the final liquid effluents were fed to a pilot scale anaerobic digester of 0.5 $ m^{3} $ volume, applying an increasing organic loading rate (OLR) regime (in terms of chemical oxygen demand (COD) from 0.2 to 15 kg COD/$ m^{3} $/day). The optimum conditions for the delignification and enzymatic hydrolysis of WS were defined as 0.5 M NaOH at 50 °C for 3–5 h and 15 μL Cellic CTec2/g pretreated straw at 50 °C. It was proven that the resulting liquid effluents could be fed to an anaerobic digester in the ratio that they are produced with satisfactory COD removal efficiencies (over 70%) for OLRs up to 10 kg COD/$ m^{3} $/day. This value is correspondent to a hydraulic retention time of around 7.5 days, much lower than the respective one for untreated straw (over 12 days). Anaerobic digestion Delignification Enzyme loading Particle size Scale-up Sugars yield Kontogianni, Nikoleta aut Barampouti, Elli Maria aut Mai, Sofia aut Moustakas, Konstantinos aut Malamis, Dimitris aut Loizidou, Maria aut Enthalten in Environmental science and pollution research Springer Berlin Heidelberg, 1994 28(2020), 19 vom: 28. Apr., Seite 24486-24498 (DE-627)171335805 (DE-600)1178791-0 (DE-576)038875101 0944-1344 nnns volume:28 year:2020 number:19 day:28 month:04 pages:24486-24498 https://doi.org/10.1007/s11356-020-08905-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-UMW SSG-OLC-ARC SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-FOR GBV_ILN_252 GBV_ILN_267 GBV_ILN_2018 GBV_ILN_4277 AR 28 2020 19 28 04 24486-24498 |
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10.1007/s11356-020-08905-y doi (DE-627)OLC2125671352 (DE-He213)s11356-020-08905-y-p DE-627 ger DE-627 rakwb eng 570 360 333.7 VZ 690 333.7 540 VZ BIODIV DE-30 fid Novakovic, Jelica verfasserin aut Towards upscaling the valorization of wheat straw residues: alkaline pretreatment using sodium hydroxide, enzymatic hydrolysis and biogas production 2020 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag GmbH Germany, part of Springer Nature 2020 Abstract Lignocellulosic biomass is considered as a recalcitrant substrate for anaerobic digestion due to its complex nature that limits its biological degradation. Therefore, suitable preprocessing for the improvement of the performance of conventional anaerobic digestion remains a challenge in the development of anaerobic digestion technology. The physical and chemical characteristics of wheat straw (WS), as a representative lignocellulosic biomass, have a significant impact on the anaerobic digestion process in terms of quantity and quality of the produced biogas. This study aimed at investigating the enzymatic saccharification and detoxification of straw prior to anaerobic digestion with the final objective of enhancing the performance of conventional anaerobic systems of recalcitrant fractions of agricultural waste. The experimental activity was performed in lab and pilot scale treating WS. Alkaline delignification of straw using sodium hydroxide (NaOH) was studied prior to enzymatic hydrolysis for the production of easily biodegradable sugars. After defining the optimum conditions for the pretreatment scheme, the anaerobic digestability of the effluents produced was measured. Finally, the final liquid effluents were fed to a pilot scale anaerobic digester of 0.5 $ m^{3} $ volume, applying an increasing organic loading rate (OLR) regime (in terms of chemical oxygen demand (COD) from 0.2 to 15 kg COD/$ m^{3} $/day). The optimum conditions for the delignification and enzymatic hydrolysis of WS were defined as 0.5 M NaOH at 50 °C for 3–5 h and 15 μL Cellic CTec2/g pretreated straw at 50 °C. It was proven that the resulting liquid effluents could be fed to an anaerobic digester in the ratio that they are produced with satisfactory COD removal efficiencies (over 70%) for OLRs up to 10 kg COD/$ m^{3} $/day. This value is correspondent to a hydraulic retention time of around 7.5 days, much lower than the respective one for untreated straw (over 12 days). Anaerobic digestion Delignification Enzyme loading Particle size Scale-up Sugars yield Kontogianni, Nikoleta aut Barampouti, Elli Maria aut Mai, Sofia aut Moustakas, Konstantinos aut Malamis, Dimitris aut Loizidou, Maria aut Enthalten in Environmental science and pollution research Springer Berlin Heidelberg, 1994 28(2020), 19 vom: 28. Apr., Seite 24486-24498 (DE-627)171335805 (DE-600)1178791-0 (DE-576)038875101 0944-1344 nnns volume:28 year:2020 number:19 day:28 month:04 pages:24486-24498 https://doi.org/10.1007/s11356-020-08905-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-UMW SSG-OLC-ARC SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-FOR GBV_ILN_252 GBV_ILN_267 GBV_ILN_2018 GBV_ILN_4277 AR 28 2020 19 28 04 24486-24498 |
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towards upscaling the valorization of wheat straw residues: alkaline pretreatment using sodium hydroxide, enzymatic hydrolysis and biogas production |
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Towards upscaling the valorization of wheat straw residues: alkaline pretreatment using sodium hydroxide, enzymatic hydrolysis and biogas production |
abstract |
Abstract Lignocellulosic biomass is considered as a recalcitrant substrate for anaerobic digestion due to its complex nature that limits its biological degradation. Therefore, suitable preprocessing for the improvement of the performance of conventional anaerobic digestion remains a challenge in the development of anaerobic digestion technology. The physical and chemical characteristics of wheat straw (WS), as a representative lignocellulosic biomass, have a significant impact on the anaerobic digestion process in terms of quantity and quality of the produced biogas. This study aimed at investigating the enzymatic saccharification and detoxification of straw prior to anaerobic digestion with the final objective of enhancing the performance of conventional anaerobic systems of recalcitrant fractions of agricultural waste. The experimental activity was performed in lab and pilot scale treating WS. Alkaline delignification of straw using sodium hydroxide (NaOH) was studied prior to enzymatic hydrolysis for the production of easily biodegradable sugars. After defining the optimum conditions for the pretreatment scheme, the anaerobic digestability of the effluents produced was measured. Finally, the final liquid effluents were fed to a pilot scale anaerobic digester of 0.5 $ m^{3} $ volume, applying an increasing organic loading rate (OLR) regime (in terms of chemical oxygen demand (COD) from 0.2 to 15 kg COD/$ m^{3} $/day). The optimum conditions for the delignification and enzymatic hydrolysis of WS were defined as 0.5 M NaOH at 50 °C for 3–5 h and 15 μL Cellic CTec2/g pretreated straw at 50 °C. It was proven that the resulting liquid effluents could be fed to an anaerobic digester in the ratio that they are produced with satisfactory COD removal efficiencies (over 70%) for OLRs up to 10 kg COD/$ m^{3} $/day. This value is correspondent to a hydraulic retention time of around 7.5 days, much lower than the respective one for untreated straw (over 12 days). © Springer-Verlag GmbH Germany, part of Springer Nature 2020 |
abstractGer |
Abstract Lignocellulosic biomass is considered as a recalcitrant substrate for anaerobic digestion due to its complex nature that limits its biological degradation. Therefore, suitable preprocessing for the improvement of the performance of conventional anaerobic digestion remains a challenge in the development of anaerobic digestion technology. The physical and chemical characteristics of wheat straw (WS), as a representative lignocellulosic biomass, have a significant impact on the anaerobic digestion process in terms of quantity and quality of the produced biogas. This study aimed at investigating the enzymatic saccharification and detoxification of straw prior to anaerobic digestion with the final objective of enhancing the performance of conventional anaerobic systems of recalcitrant fractions of agricultural waste. The experimental activity was performed in lab and pilot scale treating WS. Alkaline delignification of straw using sodium hydroxide (NaOH) was studied prior to enzymatic hydrolysis for the production of easily biodegradable sugars. After defining the optimum conditions for the pretreatment scheme, the anaerobic digestability of the effluents produced was measured. Finally, the final liquid effluents were fed to a pilot scale anaerobic digester of 0.5 $ m^{3} $ volume, applying an increasing organic loading rate (OLR) regime (in terms of chemical oxygen demand (COD) from 0.2 to 15 kg COD/$ m^{3} $/day). The optimum conditions for the delignification and enzymatic hydrolysis of WS were defined as 0.5 M NaOH at 50 °C for 3–5 h and 15 μL Cellic CTec2/g pretreated straw at 50 °C. It was proven that the resulting liquid effluents could be fed to an anaerobic digester in the ratio that they are produced with satisfactory COD removal efficiencies (over 70%) for OLRs up to 10 kg COD/$ m^{3} $/day. This value is correspondent to a hydraulic retention time of around 7.5 days, much lower than the respective one for untreated straw (over 12 days). © Springer-Verlag GmbH Germany, part of Springer Nature 2020 |
abstract_unstemmed |
Abstract Lignocellulosic biomass is considered as a recalcitrant substrate for anaerobic digestion due to its complex nature that limits its biological degradation. Therefore, suitable preprocessing for the improvement of the performance of conventional anaerobic digestion remains a challenge in the development of anaerobic digestion technology. The physical and chemical characteristics of wheat straw (WS), as a representative lignocellulosic biomass, have a significant impact on the anaerobic digestion process in terms of quantity and quality of the produced biogas. This study aimed at investigating the enzymatic saccharification and detoxification of straw prior to anaerobic digestion with the final objective of enhancing the performance of conventional anaerobic systems of recalcitrant fractions of agricultural waste. The experimental activity was performed in lab and pilot scale treating WS. Alkaline delignification of straw using sodium hydroxide (NaOH) was studied prior to enzymatic hydrolysis for the production of easily biodegradable sugars. After defining the optimum conditions for the pretreatment scheme, the anaerobic digestability of the effluents produced was measured. Finally, the final liquid effluents were fed to a pilot scale anaerobic digester of 0.5 $ m^{3} $ volume, applying an increasing organic loading rate (OLR) regime (in terms of chemical oxygen demand (COD) from 0.2 to 15 kg COD/$ m^{3} $/day). The optimum conditions for the delignification and enzymatic hydrolysis of WS were defined as 0.5 M NaOH at 50 °C for 3–5 h and 15 μL Cellic CTec2/g pretreated straw at 50 °C. It was proven that the resulting liquid effluents could be fed to an anaerobic digester in the ratio that they are produced with satisfactory COD removal efficiencies (over 70%) for OLRs up to 10 kg COD/$ m^{3} $/day. This value is correspondent to a hydraulic retention time of around 7.5 days, much lower than the respective one for untreated straw (over 12 days). © Springer-Verlag GmbH Germany, part of Springer Nature 2020 |
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