Simultaneous biodegradation of methyl parathion and carbofuran by a genetically engineered microorganism constructed by mini-Tn5 transposon
Abstract A genetically engineered microorganism (GEM) capable of simultaneous degrading methyl parathion (MP) and carbofuran was successfully constructed by random insertion of a methyl parathion hydrolase gene (mpd) into the chromosome of a carbofuran degrading Sphingomonas sp. CDS-1 with the mini-...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Jiang, Jiandong [verfasserIn] |
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| Format: |
Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2006 |
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| Schlagwörter: |
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| Anmerkung: |
© Springer Science+Business Media B.V. 2006 |
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| Übergeordnetes Werk: |
Enthalten in: Biodegradation - Kluwer Academic Publishers, 1990, 18(2006), 4 vom: 08. Nov. |
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| Übergeordnetes Werk: |
volume:18 ; year:2006 ; number:4 ; day:08 ; month:11 |
| Links: |
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| DOI / URN: |
10.1007/s10532-006-9075-5 |
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| Katalog-ID: |
OLC2130491278 |
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| 245 | 1 | 0 | |a Simultaneous biodegradation of methyl parathion and carbofuran by a genetically engineered microorganism constructed by mini-Tn5 transposon |
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| 520 | |a Abstract A genetically engineered microorganism (GEM) capable of simultaneous degrading methyl parathion (MP) and carbofuran was successfully constructed by random insertion of a methyl parathion hydrolase gene (mpd) into the chromosome of a carbofuran degrading Sphingomonas sp. CDS-1 with the mini-transposon system. The GEM constructed was relatively stable and cell viability and original degrading characteristic was not affected compared with the original recipient CDS-1. The effects of temperature, initial pH value, inoculum size and alternative carbon source on the biodegradation of MP and carbofuran were investigated. GEM cells could degrade MP and carbofuran efficiently in a relatively broad range of temperatures from 20 to 30°C, initial pH values from 6.0 to 9.0, and with all initial inoculation cell densities ($ 10^{5} $–$ 10^{7} $ CFU $ ml^{−1} $), even if alternative glucose existed. The optimal temperature and initial pH value for GEM cells to simultaneously degrade MP and carbofuran was at 30°C and at pH 7.0. The removal of MP and carbofuran by GEM cells in sterile and non-sterile soil were also studied. In both soil samples, 50 mg $ kg^{−1} $ MP and 25 mg $ kg^{−1} $ carbofuran could be degraded to an undetectable level within 25 days even if there were indigenous microbial competition and carbon sources effect. In sterile soil, the biodegradation rates of MP and carbofuran were faster, and the decline of the inoculated GEM cells was slower compared with that in non-sterile soil. The GEM constructed in this study was potential useful for pesticides bioremediation in natural environment. | ||
| 650 | 4 | |a Biodegradation | |
| 650 | 4 | |a Carbofuran | |
| 650 | 4 | |a Genetically engineered microorganism | |
| 650 | 4 | |a Methyl parathion | |
| 650 | 4 | |a Mini-transposon | |
| 700 | 1 | |a Zhang, Ruifu |4 aut | |
| 700 | 1 | |a Li, Rong |4 aut | |
| 700 | 1 | |a Gu, Ji-Dong |4 aut | |
| 700 | 1 | |a Li, Shunpeng |4 aut | |
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10.1007/s10532-006-9075-5 doi (DE-627)OLC2130491278 (DE-He213)s10532-006-9075-5-p DE-627 ger DE-627 rakwb eng 570 610 VZ 12 ssgn Jiang, Jiandong verfasserin aut Simultaneous biodegradation of methyl parathion and carbofuran by a genetically engineered microorganism constructed by mini-Tn5 transposon 2006 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer Science+Business Media B.V. 2006 Abstract A genetically engineered microorganism (GEM) capable of simultaneous degrading methyl parathion (MP) and carbofuran was successfully constructed by random insertion of a methyl parathion hydrolase gene (mpd) into the chromosome of a carbofuran degrading Sphingomonas sp. CDS-1 with the mini-transposon system. The GEM constructed was relatively stable and cell viability and original degrading characteristic was not affected compared with the original recipient CDS-1. The effects of temperature, initial pH value, inoculum size and alternative carbon source on the biodegradation of MP and carbofuran were investigated. GEM cells could degrade MP and carbofuran efficiently in a relatively broad range of temperatures from 20 to 30°C, initial pH values from 6.0 to 9.0, and with all initial inoculation cell densities ($ 10^{5} $–$ 10^{7} $ CFU $ ml^{−1} $), even if alternative glucose existed. The optimal temperature and initial pH value for GEM cells to simultaneously degrade MP and carbofuran was at 30°C and at pH 7.0. The removal of MP and carbofuran by GEM cells in sterile and non-sterile soil were also studied. In both soil samples, 50 mg $ kg^{−1} $ MP and 25 mg $ kg^{−1} $ carbofuran could be degraded to an undetectable level within 25 days even if there were indigenous microbial competition and carbon sources effect. In sterile soil, the biodegradation rates of MP and carbofuran were faster, and the decline of the inoculated GEM cells was slower compared with that in non-sterile soil. The GEM constructed in this study was potential useful for pesticides bioremediation in natural environment. Biodegradation Carbofuran Genetically engineered microorganism Methyl parathion Mini-transposon Zhang, Ruifu aut Li, Rong aut Gu, Ji-Dong aut Li, Shunpeng aut Enthalten in Biodegradation Kluwer Academic Publishers, 1990 18(2006), 4 vom: 08. Nov. (DE-627)130929395 (DE-600)1056014-2 (DE-576)026322242 0923-9820 nnns volume:18 year:2006 number:4 day:08 month:11 https://doi.org/10.1007/s10532-006-9075-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_31 GBV_ILN_70 GBV_ILN_252 GBV_ILN_2016 GBV_ILN_2360 GBV_ILN_4012 GBV_ILN_4082 GBV_ILN_4219 AR 18 2006 4 08 11 |
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10.1007/s10532-006-9075-5 doi (DE-627)OLC2130491278 (DE-He213)s10532-006-9075-5-p DE-627 ger DE-627 rakwb eng 570 610 VZ 12 ssgn Jiang, Jiandong verfasserin aut Simultaneous biodegradation of methyl parathion and carbofuran by a genetically engineered microorganism constructed by mini-Tn5 transposon 2006 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer Science+Business Media B.V. 2006 Abstract A genetically engineered microorganism (GEM) capable of simultaneous degrading methyl parathion (MP) and carbofuran was successfully constructed by random insertion of a methyl parathion hydrolase gene (mpd) into the chromosome of a carbofuran degrading Sphingomonas sp. CDS-1 with the mini-transposon system. The GEM constructed was relatively stable and cell viability and original degrading characteristic was not affected compared with the original recipient CDS-1. The effects of temperature, initial pH value, inoculum size and alternative carbon source on the biodegradation of MP and carbofuran were investigated. GEM cells could degrade MP and carbofuran efficiently in a relatively broad range of temperatures from 20 to 30°C, initial pH values from 6.0 to 9.0, and with all initial inoculation cell densities ($ 10^{5} $–$ 10^{7} $ CFU $ ml^{−1} $), even if alternative glucose existed. The optimal temperature and initial pH value for GEM cells to simultaneously degrade MP and carbofuran was at 30°C and at pH 7.0. The removal of MP and carbofuran by GEM cells in sterile and non-sterile soil were also studied. In both soil samples, 50 mg $ kg^{−1} $ MP and 25 mg $ kg^{−1} $ carbofuran could be degraded to an undetectable level within 25 days even if there were indigenous microbial competition and carbon sources effect. In sterile soil, the biodegradation rates of MP and carbofuran were faster, and the decline of the inoculated GEM cells was slower compared with that in non-sterile soil. The GEM constructed in this study was potential useful for pesticides bioremediation in natural environment. Biodegradation Carbofuran Genetically engineered microorganism Methyl parathion Mini-transposon Zhang, Ruifu aut Li, Rong aut Gu, Ji-Dong aut Li, Shunpeng aut Enthalten in Biodegradation Kluwer Academic Publishers, 1990 18(2006), 4 vom: 08. Nov. (DE-627)130929395 (DE-600)1056014-2 (DE-576)026322242 0923-9820 nnns volume:18 year:2006 number:4 day:08 month:11 https://doi.org/10.1007/s10532-006-9075-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_31 GBV_ILN_70 GBV_ILN_252 GBV_ILN_2016 GBV_ILN_2360 GBV_ILN_4012 GBV_ILN_4082 GBV_ILN_4219 AR 18 2006 4 08 11 |
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10.1007/s10532-006-9075-5 doi (DE-627)OLC2130491278 (DE-He213)s10532-006-9075-5-p DE-627 ger DE-627 rakwb eng 570 610 VZ 12 ssgn Jiang, Jiandong verfasserin aut Simultaneous biodegradation of methyl parathion and carbofuran by a genetically engineered microorganism constructed by mini-Tn5 transposon 2006 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer Science+Business Media B.V. 2006 Abstract A genetically engineered microorganism (GEM) capable of simultaneous degrading methyl parathion (MP) and carbofuran was successfully constructed by random insertion of a methyl parathion hydrolase gene (mpd) into the chromosome of a carbofuran degrading Sphingomonas sp. CDS-1 with the mini-transposon system. The GEM constructed was relatively stable and cell viability and original degrading characteristic was not affected compared with the original recipient CDS-1. The effects of temperature, initial pH value, inoculum size and alternative carbon source on the biodegradation of MP and carbofuran were investigated. GEM cells could degrade MP and carbofuran efficiently in a relatively broad range of temperatures from 20 to 30°C, initial pH values from 6.0 to 9.0, and with all initial inoculation cell densities ($ 10^{5} $–$ 10^{7} $ CFU $ ml^{−1} $), even if alternative glucose existed. The optimal temperature and initial pH value for GEM cells to simultaneously degrade MP and carbofuran was at 30°C and at pH 7.0. The removal of MP and carbofuran by GEM cells in sterile and non-sterile soil were also studied. In both soil samples, 50 mg $ kg^{−1} $ MP and 25 mg $ kg^{−1} $ carbofuran could be degraded to an undetectable level within 25 days even if there were indigenous microbial competition and carbon sources effect. In sterile soil, the biodegradation rates of MP and carbofuran were faster, and the decline of the inoculated GEM cells was slower compared with that in non-sterile soil. The GEM constructed in this study was potential useful for pesticides bioremediation in natural environment. Biodegradation Carbofuran Genetically engineered microorganism Methyl parathion Mini-transposon Zhang, Ruifu aut Li, Rong aut Gu, Ji-Dong aut Li, Shunpeng aut Enthalten in Biodegradation Kluwer Academic Publishers, 1990 18(2006), 4 vom: 08. Nov. (DE-627)130929395 (DE-600)1056014-2 (DE-576)026322242 0923-9820 nnns volume:18 year:2006 number:4 day:08 month:11 https://doi.org/10.1007/s10532-006-9075-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_31 GBV_ILN_70 GBV_ILN_252 GBV_ILN_2016 GBV_ILN_2360 GBV_ILN_4012 GBV_ILN_4082 GBV_ILN_4219 AR 18 2006 4 08 11 |
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10.1007/s10532-006-9075-5 doi (DE-627)OLC2130491278 (DE-He213)s10532-006-9075-5-p DE-627 ger DE-627 rakwb eng 570 610 VZ 12 ssgn Jiang, Jiandong verfasserin aut Simultaneous biodegradation of methyl parathion and carbofuran by a genetically engineered microorganism constructed by mini-Tn5 transposon 2006 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer Science+Business Media B.V. 2006 Abstract A genetically engineered microorganism (GEM) capable of simultaneous degrading methyl parathion (MP) and carbofuran was successfully constructed by random insertion of a methyl parathion hydrolase gene (mpd) into the chromosome of a carbofuran degrading Sphingomonas sp. CDS-1 with the mini-transposon system. The GEM constructed was relatively stable and cell viability and original degrading characteristic was not affected compared with the original recipient CDS-1. The effects of temperature, initial pH value, inoculum size and alternative carbon source on the biodegradation of MP and carbofuran were investigated. GEM cells could degrade MP and carbofuran efficiently in a relatively broad range of temperatures from 20 to 30°C, initial pH values from 6.0 to 9.0, and with all initial inoculation cell densities ($ 10^{5} $–$ 10^{7} $ CFU $ ml^{−1} $), even if alternative glucose existed. The optimal temperature and initial pH value for GEM cells to simultaneously degrade MP and carbofuran was at 30°C and at pH 7.0. The removal of MP and carbofuran by GEM cells in sterile and non-sterile soil were also studied. In both soil samples, 50 mg $ kg^{−1} $ MP and 25 mg $ kg^{−1} $ carbofuran could be degraded to an undetectable level within 25 days even if there were indigenous microbial competition and carbon sources effect. In sterile soil, the biodegradation rates of MP and carbofuran were faster, and the decline of the inoculated GEM cells was slower compared with that in non-sterile soil. The GEM constructed in this study was potential useful for pesticides bioremediation in natural environment. Biodegradation Carbofuran Genetically engineered microorganism Methyl parathion Mini-transposon Zhang, Ruifu aut Li, Rong aut Gu, Ji-Dong aut Li, Shunpeng aut Enthalten in Biodegradation Kluwer Academic Publishers, 1990 18(2006), 4 vom: 08. Nov. (DE-627)130929395 (DE-600)1056014-2 (DE-576)026322242 0923-9820 nnns volume:18 year:2006 number:4 day:08 month:11 https://doi.org/10.1007/s10532-006-9075-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_31 GBV_ILN_70 GBV_ILN_252 GBV_ILN_2016 GBV_ILN_2360 GBV_ILN_4012 GBV_ILN_4082 GBV_ILN_4219 AR 18 2006 4 08 11 |
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10.1007/s10532-006-9075-5 doi (DE-627)OLC2130491278 (DE-He213)s10532-006-9075-5-p DE-627 ger DE-627 rakwb eng 570 610 VZ 12 ssgn Jiang, Jiandong verfasserin aut Simultaneous biodegradation of methyl parathion and carbofuran by a genetically engineered microorganism constructed by mini-Tn5 transposon 2006 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer Science+Business Media B.V. 2006 Abstract A genetically engineered microorganism (GEM) capable of simultaneous degrading methyl parathion (MP) and carbofuran was successfully constructed by random insertion of a methyl parathion hydrolase gene (mpd) into the chromosome of a carbofuran degrading Sphingomonas sp. CDS-1 with the mini-transposon system. The GEM constructed was relatively stable and cell viability and original degrading characteristic was not affected compared with the original recipient CDS-1. The effects of temperature, initial pH value, inoculum size and alternative carbon source on the biodegradation of MP and carbofuran were investigated. GEM cells could degrade MP and carbofuran efficiently in a relatively broad range of temperatures from 20 to 30°C, initial pH values from 6.0 to 9.0, and with all initial inoculation cell densities ($ 10^{5} $–$ 10^{7} $ CFU $ ml^{−1} $), even if alternative glucose existed. The optimal temperature and initial pH value for GEM cells to simultaneously degrade MP and carbofuran was at 30°C and at pH 7.0. The removal of MP and carbofuran by GEM cells in sterile and non-sterile soil were also studied. In both soil samples, 50 mg $ kg^{−1} $ MP and 25 mg $ kg^{−1} $ carbofuran could be degraded to an undetectable level within 25 days even if there were indigenous microbial competition and carbon sources effect. In sterile soil, the biodegradation rates of MP and carbofuran were faster, and the decline of the inoculated GEM cells was slower compared with that in non-sterile soil. The GEM constructed in this study was potential useful for pesticides bioremediation in natural environment. Biodegradation Carbofuran Genetically engineered microorganism Methyl parathion Mini-transposon Zhang, Ruifu aut Li, Rong aut Gu, Ji-Dong aut Li, Shunpeng aut Enthalten in Biodegradation Kluwer Academic Publishers, 1990 18(2006), 4 vom: 08. Nov. (DE-627)130929395 (DE-600)1056014-2 (DE-576)026322242 0923-9820 nnns volume:18 year:2006 number:4 day:08 month:11 https://doi.org/10.1007/s10532-006-9075-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_31 GBV_ILN_70 GBV_ILN_252 GBV_ILN_2016 GBV_ILN_2360 GBV_ILN_4012 GBV_ILN_4082 GBV_ILN_4219 AR 18 2006 4 08 11 |
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English |
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Enthalten in Biodegradation 18(2006), 4 vom: 08. Nov. volume:18 year:2006 number:4 day:08 month:11 |
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Enthalten in Biodegradation 18(2006), 4 vom: 08. Nov. volume:18 year:2006 number:4 day:08 month:11 |
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Jiang, Jiandong @@aut@@ Zhang, Ruifu @@aut@@ Li, Rong @@aut@@ Gu, Ji-Dong @@aut@@ Li, Shunpeng @@aut@@ |
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simultaneous biodegradation of methyl parathion and carbofuran by a genetically engineered microorganism constructed by mini-tn5 transposon |
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Simultaneous biodegradation of methyl parathion and carbofuran by a genetically engineered microorganism constructed by mini-Tn5 transposon |
| abstract |
Abstract A genetically engineered microorganism (GEM) capable of simultaneous degrading methyl parathion (MP) and carbofuran was successfully constructed by random insertion of a methyl parathion hydrolase gene (mpd) into the chromosome of a carbofuran degrading Sphingomonas sp. CDS-1 with the mini-transposon system. The GEM constructed was relatively stable and cell viability and original degrading characteristic was not affected compared with the original recipient CDS-1. The effects of temperature, initial pH value, inoculum size and alternative carbon source on the biodegradation of MP and carbofuran were investigated. GEM cells could degrade MP and carbofuran efficiently in a relatively broad range of temperatures from 20 to 30°C, initial pH values from 6.0 to 9.0, and with all initial inoculation cell densities ($ 10^{5} $–$ 10^{7} $ CFU $ ml^{−1} $), even if alternative glucose existed. The optimal temperature and initial pH value for GEM cells to simultaneously degrade MP and carbofuran was at 30°C and at pH 7.0. The removal of MP and carbofuran by GEM cells in sterile and non-sterile soil were also studied. In both soil samples, 50 mg $ kg^{−1} $ MP and 25 mg $ kg^{−1} $ carbofuran could be degraded to an undetectable level within 25 days even if there were indigenous microbial competition and carbon sources effect. In sterile soil, the biodegradation rates of MP and carbofuran were faster, and the decline of the inoculated GEM cells was slower compared with that in non-sterile soil. The GEM constructed in this study was potential useful for pesticides bioremediation in natural environment. © Springer Science+Business Media B.V. 2006 |
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Abstract A genetically engineered microorganism (GEM) capable of simultaneous degrading methyl parathion (MP) and carbofuran was successfully constructed by random insertion of a methyl parathion hydrolase gene (mpd) into the chromosome of a carbofuran degrading Sphingomonas sp. CDS-1 with the mini-transposon system. The GEM constructed was relatively stable and cell viability and original degrading characteristic was not affected compared with the original recipient CDS-1. The effects of temperature, initial pH value, inoculum size and alternative carbon source on the biodegradation of MP and carbofuran were investigated. GEM cells could degrade MP and carbofuran efficiently in a relatively broad range of temperatures from 20 to 30°C, initial pH values from 6.0 to 9.0, and with all initial inoculation cell densities ($ 10^{5} $–$ 10^{7} $ CFU $ ml^{−1} $), even if alternative glucose existed. The optimal temperature and initial pH value for GEM cells to simultaneously degrade MP and carbofuran was at 30°C and at pH 7.0. The removal of MP and carbofuran by GEM cells in sterile and non-sterile soil were also studied. In both soil samples, 50 mg $ kg^{−1} $ MP and 25 mg $ kg^{−1} $ carbofuran could be degraded to an undetectable level within 25 days even if there were indigenous microbial competition and carbon sources effect. In sterile soil, the biodegradation rates of MP and carbofuran were faster, and the decline of the inoculated GEM cells was slower compared with that in non-sterile soil. The GEM constructed in this study was potential useful for pesticides bioremediation in natural environment. © Springer Science+Business Media B.V. 2006 |
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Abstract A genetically engineered microorganism (GEM) capable of simultaneous degrading methyl parathion (MP) and carbofuran was successfully constructed by random insertion of a methyl parathion hydrolase gene (mpd) into the chromosome of a carbofuran degrading Sphingomonas sp. CDS-1 with the mini-transposon system. The GEM constructed was relatively stable and cell viability and original degrading characteristic was not affected compared with the original recipient CDS-1. The effects of temperature, initial pH value, inoculum size and alternative carbon source on the biodegradation of MP and carbofuran were investigated. GEM cells could degrade MP and carbofuran efficiently in a relatively broad range of temperatures from 20 to 30°C, initial pH values from 6.0 to 9.0, and with all initial inoculation cell densities ($ 10^{5} $–$ 10^{7} $ CFU $ ml^{−1} $), even if alternative glucose existed. The optimal temperature and initial pH value for GEM cells to simultaneously degrade MP and carbofuran was at 30°C and at pH 7.0. The removal of MP and carbofuran by GEM cells in sterile and non-sterile soil were also studied. In both soil samples, 50 mg $ kg^{−1} $ MP and 25 mg $ kg^{−1} $ carbofuran could be degraded to an undetectable level within 25 days even if there were indigenous microbial competition and carbon sources effect. In sterile soil, the biodegradation rates of MP and carbofuran were faster, and the decline of the inoculated GEM cells was slower compared with that in non-sterile soil. The GEM constructed in this study was potential useful for pesticides bioremediation in natural environment. © Springer Science+Business Media B.V. 2006 |
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