Purification and characterization of two low molecular weight endoglucanases produced by Penicillium occitanis mutant pol 6

Abstract Two endoglucanases (EGs), EG A and EG B, were purified to homogeneity from Penicillium occitanis mutant Pol6 culture medium. The molecular weights of EGA and EGB were 31,000 and 28,000 kDa, respectively. The pI was about 3 for EG A and 7.5 for EG B. Optimal activity was obtained at pH 3.5 f...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Chaabouni, Semia Ellouz [verfasserIn] Mechichi, Tahar Limam, Ferid Marzouki, Nejib

Format:	Artikel
Sprache:	Englisch

Erschienen:	2005

Anmerkung:	© Humana Press Inc 2005

Übergeordnetes Werk:	Enthalten in: Applied biochemistry and biotechnology / A - Humana Press, 1994, 125(2005), 2 vom: Mai, Seite 99-112
Übergeordnetes Werk:	volume:125 ; year:2005 ; number:2 ; month:05 ; pages:99-112

Links:	Volltext

DOI / URN:	10.1385/ABAB:125:2:099

Katalog-ID:	OLC2130512496

Internformat


LEADER	01000naa a22002652 4500
001	OLC2130512496
003	DE-627
005	20230506021542.0
007	tu
008	230506s2005 xx \|\|\|\|\| 00\| \|\|eng c
024	7		\|a 10.1385/ABAB:125:2:099 \|2 doi
035			\|a (DE-627)OLC2130512496
035			\|a (DE-He213)ABAB:125:2:099-p
040			\|a DE-627 \|b ger \|c DE-627 \|e rakwb
041			\|a eng
082	0	4	\|a 570 \|a 540 \|a 660 \|q VZ
084			\|a 12 \|2 ssgn
084			\|a 42.00 \|2 bkl
100	1		\|a Chaabouni, Semia Ellouz \|e verfasserin \|4 aut
245	1	0	\|a Purification and characterization of two low molecular weight endoglucanases produced by Penicillium occitanis mutant pol 6
264		1	\|c 2005
336			\|a Text \|b txt \|2 rdacontent
337			\|a ohne Hilfsmittel zu benutzen \|b n \|2 rdamedia
338			\|a Band \|b nc \|2 rdacarrier
500			\|a © Humana Press Inc 2005
520			\|a Abstract Two endoglucanases (EGs), EG A and EG B, were purified to homogeneity from Penicillium occitanis mutant Pol6 culture medium. The molecular weights of EGA and EGB were 31,000 and 28,000 kDa, respectively. The pI was about 3 for EG A and 7.5 for EG B. Optimal activity was obtained at pH 3.5 for both endoglucanases. Optimal temperature for enzyme activity was 60°C for EG A and 50°C for EG B. EG A was thermostable at 60°C and remained active after 1 h at 70°C. EGs hydrolyzed carboxymethylcellulose, phosphoric acid swollen cellulose, and ß-glucan efficiently, whereas microcrystalline cellulose (Avicel) and laminarin were poorly hydrolyzed. Only EG B showed xylanase activity. Furthermore, these EGs were insensitive to the action of glucose and cellobiose but were inhibited by the divalent cations $ Hg^{2+} $, $ Co^{2+} $, and $ Mn^{2+} $.
700	1		\|a Mechichi, Tahar \|4 aut
700	1		\|a Limam, Ferid \|4 aut
700	1		\|a Marzouki, Nejib \|4 aut
773	0	8	\|i Enthalten in \|t Applied biochemistry and biotechnology / A \|d Humana Press, 1994 \|g 125(2005), 2 vom: Mai, Seite 99-112 \|w (DE-627)182278573 \|w (DE-600)1193054-8 \|w (DE-576)043085105 \|x 0273-2289 \|7 nnns
773	1	8	\|g volume:125 \|g year:2005 \|g number:2 \|g month:05 \|g pages:99-112
856	4	1	\|u https://doi.org/10.1385/ABAB:125:2:099 \|z lizenzpflichtig \|3 Volltext
912			\|a GBV_USEFLAG_A
912			\|a SYSFLAG_A
912			\|a GBV_OLC
912			\|a SSG-OLC-TEC
912			\|a SSG-OLC-CHE
912			\|a GBV_ILN_21
912			\|a GBV_ILN_40
912			\|a GBV_ILN_65
912			\|a GBV_ILN_70
912			\|a GBV_ILN_105
912			\|a GBV_ILN_4012
912			\|a GBV_ILN_4219
912			\|a GBV_ILN_4310
936	b	k	\|a 42.00 \|q VZ
951			\|a AR
952			\|d 125 \|j 2005 \|e 2 \|c 05 \|h 99-112

Indexfelder

author_variant	s e c se sec t m tm f l fl n m nm
matchkey_str	article:02732289:2005----::uiiainncaatrztootoomlclregtnolcnssrdcdye
hierarchy_sort_str	2005
bklnumber	42.00
publishDate	2005
allfields	10.1385/ABAB:125:2:099 doi (DE-627)OLC2130512496 (DE-He213)ABAB:125:2:099-p DE-627 ger DE-627 rakwb eng 570 540 660 VZ 12 ssgn 42.00 bkl Chaabouni, Semia Ellouz verfasserin aut Purification and characterization of two low molecular weight endoglucanases produced by Penicillium occitanis mutant pol 6 2005 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Humana Press Inc 2005 Abstract Two endoglucanases (EGs), EG A and EG B, were purified to homogeneity from Penicillium occitanis mutant Pol6 culture medium. The molecular weights of EGA and EGB were 31,000 and 28,000 kDa, respectively. The pI was about 3 for EG A and 7.5 for EG B. Optimal activity was obtained at pH 3.5 for both endoglucanases. Optimal temperature for enzyme activity was 60°C for EG A and 50°C for EG B. EG A was thermostable at 60°C and remained active after 1 h at 70°C. EGs hydrolyzed carboxymethylcellulose, phosphoric acid swollen cellulose, and ß-glucan efficiently, whereas microcrystalline cellulose (Avicel) and laminarin were poorly hydrolyzed. Only EG B showed xylanase activity. Furthermore, these EGs were insensitive to the action of glucose and cellobiose but were inhibited by the divalent cations $ Hg^{2+} $, $ Co^{2+} $, and $ Mn^{2+} $. Mechichi, Tahar aut Limam, Ferid aut Marzouki, Nejib aut Enthalten in Applied biochemistry and biotechnology / A Humana Press, 1994 125(2005), 2 vom: Mai, Seite 99-112 (DE-627)182278573 (DE-600)1193054-8 (DE-576)043085105 0273-2289 nnns volume:125 year:2005 number:2 month:05 pages:99-112 https://doi.org/10.1385/ABAB:125:2:099 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_21 GBV_ILN_40 GBV_ILN_65 GBV_ILN_70 GBV_ILN_105 GBV_ILN_4012 GBV_ILN_4219 GBV_ILN_4310 42.00 VZ AR 125 2005 2 05 99-112
spelling	10.1385/ABAB:125:2:099 doi (DE-627)OLC2130512496 (DE-He213)ABAB:125:2:099-p DE-627 ger DE-627 rakwb eng 570 540 660 VZ 12 ssgn 42.00 bkl Chaabouni, Semia Ellouz verfasserin aut Purification and characterization of two low molecular weight endoglucanases produced by Penicillium occitanis mutant pol 6 2005 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Humana Press Inc 2005 Abstract Two endoglucanases (EGs), EG A and EG B, were purified to homogeneity from Penicillium occitanis mutant Pol6 culture medium. The molecular weights of EGA and EGB were 31,000 and 28,000 kDa, respectively. The pI was about 3 for EG A and 7.5 for EG B. Optimal activity was obtained at pH 3.5 for both endoglucanases. Optimal temperature for enzyme activity was 60°C for EG A and 50°C for EG B. EG A was thermostable at 60°C and remained active after 1 h at 70°C. EGs hydrolyzed carboxymethylcellulose, phosphoric acid swollen cellulose, and ß-glucan efficiently, whereas microcrystalline cellulose (Avicel) and laminarin were poorly hydrolyzed. Only EG B showed xylanase activity. Furthermore, these EGs were insensitive to the action of glucose and cellobiose but were inhibited by the divalent cations $ Hg^{2+} $, $ Co^{2+} $, and $ Mn^{2+} $. Mechichi, Tahar aut Limam, Ferid aut Marzouki, Nejib aut Enthalten in Applied biochemistry and biotechnology / A Humana Press, 1994 125(2005), 2 vom: Mai, Seite 99-112 (DE-627)182278573 (DE-600)1193054-8 (DE-576)043085105 0273-2289 nnns volume:125 year:2005 number:2 month:05 pages:99-112 https://doi.org/10.1385/ABAB:125:2:099 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_21 GBV_ILN_40 GBV_ILN_65 GBV_ILN_70 GBV_ILN_105 GBV_ILN_4012 GBV_ILN_4219 GBV_ILN_4310 42.00 VZ AR 125 2005 2 05 99-112
allfields_unstemmed	10.1385/ABAB:125:2:099 doi (DE-627)OLC2130512496 (DE-He213)ABAB:125:2:099-p DE-627 ger DE-627 rakwb eng 570 540 660 VZ 12 ssgn 42.00 bkl Chaabouni, Semia Ellouz verfasserin aut Purification and characterization of two low molecular weight endoglucanases produced by Penicillium occitanis mutant pol 6 2005 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Humana Press Inc 2005 Abstract Two endoglucanases (EGs), EG A and EG B, were purified to homogeneity from Penicillium occitanis mutant Pol6 culture medium. The molecular weights of EGA and EGB were 31,000 and 28,000 kDa, respectively. The pI was about 3 for EG A and 7.5 for EG B. Optimal activity was obtained at pH 3.5 for both endoglucanases. Optimal temperature for enzyme activity was 60°C for EG A and 50°C for EG B. EG A was thermostable at 60°C and remained active after 1 h at 70°C. EGs hydrolyzed carboxymethylcellulose, phosphoric acid swollen cellulose, and ß-glucan efficiently, whereas microcrystalline cellulose (Avicel) and laminarin were poorly hydrolyzed. Only EG B showed xylanase activity. Furthermore, these EGs were insensitive to the action of glucose and cellobiose but were inhibited by the divalent cations $ Hg^{2+} $, $ Co^{2+} $, and $ Mn^{2+} $. Mechichi, Tahar aut Limam, Ferid aut Marzouki, Nejib aut Enthalten in Applied biochemistry and biotechnology / A Humana Press, 1994 125(2005), 2 vom: Mai, Seite 99-112 (DE-627)182278573 (DE-600)1193054-8 (DE-576)043085105 0273-2289 nnns volume:125 year:2005 number:2 month:05 pages:99-112 https://doi.org/10.1385/ABAB:125:2:099 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_21 GBV_ILN_40 GBV_ILN_65 GBV_ILN_70 GBV_ILN_105 GBV_ILN_4012 GBV_ILN_4219 GBV_ILN_4310 42.00 VZ AR 125 2005 2 05 99-112
allfieldsGer	10.1385/ABAB:125:2:099 doi (DE-627)OLC2130512496 (DE-He213)ABAB:125:2:099-p DE-627 ger DE-627 rakwb eng 570 540 660 VZ 12 ssgn 42.00 bkl Chaabouni, Semia Ellouz verfasserin aut Purification and characterization of two low molecular weight endoglucanases produced by Penicillium occitanis mutant pol 6 2005 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Humana Press Inc 2005 Abstract Two endoglucanases (EGs), EG A and EG B, were purified to homogeneity from Penicillium occitanis mutant Pol6 culture medium. The molecular weights of EGA and EGB were 31,000 and 28,000 kDa, respectively. The pI was about 3 for EG A and 7.5 for EG B. Optimal activity was obtained at pH 3.5 for both endoglucanases. Optimal temperature for enzyme activity was 60°C for EG A and 50°C for EG B. EG A was thermostable at 60°C and remained active after 1 h at 70°C. EGs hydrolyzed carboxymethylcellulose, phosphoric acid swollen cellulose, and ß-glucan efficiently, whereas microcrystalline cellulose (Avicel) and laminarin were poorly hydrolyzed. Only EG B showed xylanase activity. Furthermore, these EGs were insensitive to the action of glucose and cellobiose but were inhibited by the divalent cations $ Hg^{2+} $, $ Co^{2+} $, and $ Mn^{2+} $. Mechichi, Tahar aut Limam, Ferid aut Marzouki, Nejib aut Enthalten in Applied biochemistry and biotechnology / A Humana Press, 1994 125(2005), 2 vom: Mai, Seite 99-112 (DE-627)182278573 (DE-600)1193054-8 (DE-576)043085105 0273-2289 nnns volume:125 year:2005 number:2 month:05 pages:99-112 https://doi.org/10.1385/ABAB:125:2:099 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_21 GBV_ILN_40 GBV_ILN_65 GBV_ILN_70 GBV_ILN_105 GBV_ILN_4012 GBV_ILN_4219 GBV_ILN_4310 42.00 VZ AR 125 2005 2 05 99-112
allfieldsSound	10.1385/ABAB:125:2:099 doi (DE-627)OLC2130512496 (DE-He213)ABAB:125:2:099-p DE-627 ger DE-627 rakwb eng 570 540 660 VZ 12 ssgn 42.00 bkl Chaabouni, Semia Ellouz verfasserin aut Purification and characterization of two low molecular weight endoglucanases produced by Penicillium occitanis mutant pol 6 2005 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Humana Press Inc 2005 Abstract Two endoglucanases (EGs), EG A and EG B, were purified to homogeneity from Penicillium occitanis mutant Pol6 culture medium. The molecular weights of EGA and EGB were 31,000 and 28,000 kDa, respectively. The pI was about 3 for EG A and 7.5 for EG B. Optimal activity was obtained at pH 3.5 for both endoglucanases. Optimal temperature for enzyme activity was 60°C for EG A and 50°C for EG B. EG A was thermostable at 60°C and remained active after 1 h at 70°C. EGs hydrolyzed carboxymethylcellulose, phosphoric acid swollen cellulose, and ß-glucan efficiently, whereas microcrystalline cellulose (Avicel) and laminarin were poorly hydrolyzed. Only EG B showed xylanase activity. Furthermore, these EGs were insensitive to the action of glucose and cellobiose but were inhibited by the divalent cations $ Hg^{2+} $, $ Co^{2+} $, and $ Mn^{2+} $. Mechichi, Tahar aut Limam, Ferid aut Marzouki, Nejib aut Enthalten in Applied biochemistry and biotechnology / A Humana Press, 1994 125(2005), 2 vom: Mai, Seite 99-112 (DE-627)182278573 (DE-600)1193054-8 (DE-576)043085105 0273-2289 nnns volume:125 year:2005 number:2 month:05 pages:99-112 https://doi.org/10.1385/ABAB:125:2:099 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_21 GBV_ILN_40 GBV_ILN_65 GBV_ILN_70 GBV_ILN_105 GBV_ILN_4012 GBV_ILN_4219 GBV_ILN_4310 42.00 VZ AR 125 2005 2 05 99-112
language	English
source	Enthalten in Applied biochemistry and biotechnology / A 125(2005), 2 vom: Mai, Seite 99-112 volume:125 year:2005 number:2 month:05 pages:99-112
sourceStr	Enthalten in Applied biochemistry and biotechnology / A 125(2005), 2 vom: Mai, Seite 99-112 volume:125 year:2005 number:2 month:05 pages:99-112
format_phy_str_mv	Article
institution	findex.gbv.de
dewey-raw	570
isfreeaccess_bool	false
container_title	Applied biochemistry and biotechnology / A
authorswithroles_txt_mv	Chaabouni, Semia Ellouz @@aut@@ Mechichi, Tahar @@aut@@ Limam, Ferid @@aut@@ Marzouki, Nejib @@aut@@
publishDateDaySort_date	2005-05-01T00:00:00Z
hierarchy_top_id	182278573
dewey-sort	3570
id	OLC2130512496
language_de	englisch
fullrecord	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000naa a22002652 4500</leader><controlfield tag="001">OLC2130512496</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230506021542.0</controlfield><controlfield tag="007">tu</controlfield><controlfield tag="008">230506s2005 xx \|\|\|\|\| 00\| \|\|eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1385/ABAB:125:2:099</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)OLC2130512496</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-He213)ABAB:125:2:099-p</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">570</subfield><subfield code="a">540</subfield><subfield code="a">660</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">12</subfield><subfield code="2">ssgn</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">42.00</subfield><subfield code="2">bkl</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Chaabouni, Semia Ellouz</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Purification and characterization of two low molecular weight endoglucanases produced by Penicillium occitanis mutant pol 6</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2005</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">ohne Hilfsmittel zu benutzen</subfield><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Band</subfield><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">© Humana Press Inc 2005</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract Two endoglucanases (EGs), EG A and EG B, were purified to homogeneity from Penicillium occitanis mutant Pol6 culture medium. The molecular weights of EGA and EGB were 31,000 and 28,000 kDa, respectively. The pI was about 3 for EG A and 7.5 for EG B. Optimal activity was obtained at pH 3.5 for both endoglucanases. Optimal temperature for enzyme activity was 60°C for EG A and 50°C for EG B. EG A was thermostable at 60°C and remained active after 1 h at 70°C. EGs hydrolyzed carboxymethylcellulose, phosphoric acid swollen cellulose, and ß-glucan efficiently, whereas microcrystalline cellulose (Avicel) and laminarin were poorly hydrolyzed. Only EG B showed xylanase activity. Furthermore, these EGs were insensitive to the action of glucose and cellobiose but were inhibited by the divalent cations $ Hg^{2+} $, $ Co^{2+} $, and $ Mn^{2+} $.</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Mechichi, Tahar</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Limam, Ferid</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Marzouki, Nejib</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Applied biochemistry and biotechnology / A</subfield><subfield code="d">Humana Press, 1994</subfield><subfield code="g">125(2005), 2 vom: Mai, Seite 99-112</subfield><subfield code="w">(DE-627)182278573</subfield><subfield code="w">(DE-600)1193054-8</subfield><subfield code="w">(DE-576)043085105</subfield><subfield code="x">0273-2289</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:125</subfield><subfield code="g">year:2005</subfield><subfield code="g">number:2</subfield><subfield code="g">month:05</subfield><subfield code="g">pages:99-112</subfield></datafield><datafield tag="856" ind1="4" ind2="1"><subfield code="u">https://doi.org/10.1385/ABAB:125:2:099</subfield><subfield code="z">lizenzpflichtig</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_OLC</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-TEC</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-CHE</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_21</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_40</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_65</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_70</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_105</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4012</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4219</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4310</subfield></datafield><datafield tag="936" ind1="b" ind2="k"><subfield code="a">42.00</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">125</subfield><subfield code="j">2005</subfield><subfield code="e">2</subfield><subfield code="c">05</subfield><subfield code="h">99-112</subfield></datafield></record></collection>
author	Chaabouni, Semia Ellouz
spellingShingle	Chaabouni, Semia Ellouz ddc 570 ssgn 12 bkl 42.00 Purification and characterization of two low molecular weight endoglucanases produced by Penicillium occitanis mutant pol 6
authorStr	Chaabouni, Semia Ellouz
ppnlink_with_tag_str_mv	@@773@@(DE-627)182278573
format	Article
dewey-ones	570 - Life sciences; biology 540 - Chemistry & allied sciences 660 - Chemical engineering
delete_txt_mv	keep
author_role	aut aut aut aut
collection	OLC
remote_str	false
illustrated	Not Illustrated
issn	0273-2289
topic_title	570 540 660 VZ 12 ssgn 42.00 bkl Purification and characterization of two low molecular weight endoglucanases produced by Penicillium occitanis mutant pol 6
topic	ddc 570 ssgn 12 bkl 42.00
topic_unstemmed	ddc 570 ssgn 12 bkl 42.00
topic_browse	ddc 570 ssgn 12 bkl 42.00
format_facet	Aufsätze Gedruckte Aufsätze
format_main_str_mv	Text Zeitschrift/Artikel
carriertype_str_mv	nc
hierarchy_parent_title	Applied biochemistry and biotechnology / A
hierarchy_parent_id	182278573
dewey-tens	570 - Life sciences; biology 540 - Chemistry 660 - Chemical engineering
hierarchy_top_title	Applied biochemistry and biotechnology / A
isfreeaccess_txt	false
familylinks_str_mv	(DE-627)182278573 (DE-600)1193054-8 (DE-576)043085105
title	Purification and characterization of two low molecular weight endoglucanases produced by Penicillium occitanis mutant pol 6
ctrlnum	(DE-627)OLC2130512496 (DE-He213)ABAB:125:2:099-p
title_full	Purification and characterization of two low molecular weight endoglucanases produced by Penicillium occitanis mutant pol 6
author_sort	Chaabouni, Semia Ellouz
journal	Applied biochemistry and biotechnology / A
journalStr	Applied biochemistry and biotechnology / A
lang_code	eng
isOA_bool	false
dewey-hundreds	500 - Science 600 - Technology
recordtype	marc
publishDateSort	2005
contenttype_str_mv	txt
container_start_page	99
author_browse	Chaabouni, Semia Ellouz Mechichi, Tahar Limam, Ferid Marzouki, Nejib
container_volume	125
class	570 540 660 VZ 12 ssgn 42.00 bkl
format_se	Aufsätze
author-letter	Chaabouni, Semia Ellouz
doi_str_mv	10.1385/ABAB:125:2:099
dewey-full	570 540 660
title_sort	purification and characterization of two low molecular weight endoglucanases produced by penicillium occitanis mutant pol 6
title_auth	Purification and characterization of two low molecular weight endoglucanases produced by Penicillium occitanis mutant pol 6
abstract	Abstract Two endoglucanases (EGs), EG A and EG B, were purified to homogeneity from Penicillium occitanis mutant Pol6 culture medium. The molecular weights of EGA and EGB were 31,000 and 28,000 kDa, respectively. The pI was about 3 for EG A and 7.5 for EG B. Optimal activity was obtained at pH 3.5 for both endoglucanases. Optimal temperature for enzyme activity was 60°C for EG A and 50°C for EG B. EG A was thermostable at 60°C and remained active after 1 h at 70°C. EGs hydrolyzed carboxymethylcellulose, phosphoric acid swollen cellulose, and ß-glucan efficiently, whereas microcrystalline cellulose (Avicel) and laminarin were poorly hydrolyzed. Only EG B showed xylanase activity. Furthermore, these EGs were insensitive to the action of glucose and cellobiose but were inhibited by the divalent cations $ Hg^{2+} $, $ Co^{2+} $, and $ Mn^{2+} $. © Humana Press Inc 2005
abstractGer	Abstract Two endoglucanases (EGs), EG A and EG B, were purified to homogeneity from Penicillium occitanis mutant Pol6 culture medium. The molecular weights of EGA and EGB were 31,000 and 28,000 kDa, respectively. The pI was about 3 for EG A and 7.5 for EG B. Optimal activity was obtained at pH 3.5 for both endoglucanases. Optimal temperature for enzyme activity was 60°C for EG A and 50°C for EG B. EG A was thermostable at 60°C and remained active after 1 h at 70°C. EGs hydrolyzed carboxymethylcellulose, phosphoric acid swollen cellulose, and ß-glucan efficiently, whereas microcrystalline cellulose (Avicel) and laminarin were poorly hydrolyzed. Only EG B showed xylanase activity. Furthermore, these EGs were insensitive to the action of glucose and cellobiose but were inhibited by the divalent cations $ Hg^{2+} $, $ Co^{2+} $, and $ Mn^{2+} $. © Humana Press Inc 2005
abstract_unstemmed	Abstract Two endoglucanases (EGs), EG A and EG B, were purified to homogeneity from Penicillium occitanis mutant Pol6 culture medium. The molecular weights of EGA and EGB were 31,000 and 28,000 kDa, respectively. The pI was about 3 for EG A and 7.5 for EG B. Optimal activity was obtained at pH 3.5 for both endoglucanases. Optimal temperature for enzyme activity was 60°C for EG A and 50°C for EG B. EG A was thermostable at 60°C and remained active after 1 h at 70°C. EGs hydrolyzed carboxymethylcellulose, phosphoric acid swollen cellulose, and ß-glucan efficiently, whereas microcrystalline cellulose (Avicel) and laminarin were poorly hydrolyzed. Only EG B showed xylanase activity. Furthermore, these EGs were insensitive to the action of glucose and cellobiose but were inhibited by the divalent cations $ Hg^{2+} $, $ Co^{2+} $, and $ Mn^{2+} $. © Humana Press Inc 2005
collection_details	GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_21 GBV_ILN_40 GBV_ILN_65 GBV_ILN_70 GBV_ILN_105 GBV_ILN_4012 GBV_ILN_4219 GBV_ILN_4310
container_issue	2
title_short	Purification and characterization of two low molecular weight endoglucanases produced by Penicillium occitanis mutant pol 6
url	https://doi.org/10.1385/ABAB:125:2:099
remote_bool	false
author2	Mechichi, Tahar Limam, Ferid Marzouki, Nejib
author2Str	Mechichi, Tahar Limam, Ferid Marzouki, Nejib
ppnlink	182278573
mediatype_str_mv	n
isOA_txt	false
hochschulschrift_bool	false
doi_str	10.1385/ABAB:125:2:099
up_date	2024-07-04T05:27:04.467Z
_version_	1803624984824774656
fullrecord_marcxml	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000naa a22002652 4500</leader><controlfield tag="001">OLC2130512496</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230506021542.0</controlfield><controlfield tag="007">tu</controlfield><controlfield tag="008">230506s2005 xx \|\|\|\|\| 00\| \|\|eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1385/ABAB:125:2:099</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)OLC2130512496</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-He213)ABAB:125:2:099-p</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">570</subfield><subfield code="a">540</subfield><subfield code="a">660</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">12</subfield><subfield code="2">ssgn</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">42.00</subfield><subfield code="2">bkl</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Chaabouni, Semia Ellouz</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Purification and characterization of two low molecular weight endoglucanases produced by Penicillium occitanis mutant pol 6</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2005</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">ohne Hilfsmittel zu benutzen</subfield><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Band</subfield><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">© Humana Press Inc 2005</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract Two endoglucanases (EGs), EG A and EG B, were purified to homogeneity from Penicillium occitanis mutant Pol6 culture medium. The molecular weights of EGA and EGB were 31,000 and 28,000 kDa, respectively. The pI was about 3 for EG A and 7.5 for EG B. Optimal activity was obtained at pH 3.5 for both endoglucanases. Optimal temperature for enzyme activity was 60°C for EG A and 50°C for EG B. EG A was thermostable at 60°C and remained active after 1 h at 70°C. EGs hydrolyzed carboxymethylcellulose, phosphoric acid swollen cellulose, and ß-glucan efficiently, whereas microcrystalline cellulose (Avicel) and laminarin were poorly hydrolyzed. Only EG B showed xylanase activity. Furthermore, these EGs were insensitive to the action of glucose and cellobiose but were inhibited by the divalent cations $ Hg^{2+} $, $ Co^{2+} $, and $ Mn^{2+} $.</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Mechichi, Tahar</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Limam, Ferid</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Marzouki, Nejib</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Applied biochemistry and biotechnology / A</subfield><subfield code="d">Humana Press, 1994</subfield><subfield code="g">125(2005), 2 vom: Mai, Seite 99-112</subfield><subfield code="w">(DE-627)182278573</subfield><subfield code="w">(DE-600)1193054-8</subfield><subfield code="w">(DE-576)043085105</subfield><subfield code="x">0273-2289</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:125</subfield><subfield code="g">year:2005</subfield><subfield code="g">number:2</subfield><subfield code="g">month:05</subfield><subfield code="g">pages:99-112</subfield></datafield><datafield tag="856" ind1="4" ind2="1"><subfield code="u">https://doi.org/10.1385/ABAB:125:2:099</subfield><subfield code="z">lizenzpflichtig</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_OLC</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-TEC</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-CHE</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_21</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_40</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_65</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_70</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_105</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4012</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4219</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4310</subfield></datafield><datafield tag="936" ind1="b" ind2="k"><subfield code="a">42.00</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">125</subfield><subfield code="j">2005</subfield><subfield code="e">2</subfield><subfield code="c">05</subfield><subfield code="h">99-112</subfield></datafield></record></collection>
score	7.397299

Nicht das Richtige dabei?

Schreiben Sie uns!

Purification and characterization of two low molecular weight endoglucanases produced by Penicillium occitanis mutant pol 6

Nicht das Richtige dabei?

Zugang & Verfügbarkeit

Vorhandene Bände

Nicht das Richtige dabei?