Comparison of two liquid-state NMR methods for the determination of saccharides in carrot (Daucus carota L.) roots
Abstract In order to determine the saccharide content of plant tissues, we studied a new non-destructive and fast analytical method that we call “direct quantitative proton nuclear magnetic resonance spectroscopy” (d q 1H NMR): the application of quantitative proton nuclear magnetic resonance spectr...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Weberskirch, Linda [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2010 |
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| Schlagwörter: |
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| Anmerkung: |
© Springer-Verlag 2010 |
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| Übergeordnetes Werk: |
Enthalten in: Analytical and bioanalytical chemistry - Berlin : Springer, 2002, 399(2010), 1 vom: 28. Okt., Seite 483-487 |
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| Übergeordnetes Werk: |
volume:399 ; year:2010 ; number:1 ; day:28 ; month:10 ; pages:483-487 |
| Links: |
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| DOI / URN: |
10.1007/s00216-010-4311-6 |
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| 520 | |a Abstract In order to determine the saccharide content of plant tissues, we studied a new non-destructive and fast analytical method that we call “direct quantitative proton nuclear magnetic resonance spectroscopy” (d q 1H NMR): the application of quantitative proton nuclear magnetic resonance spectroscopy (q 1H NMR) to non modified plant tissues along with capillary tubes containing a reference compound (for quantification) and deuterium oxide (for locking). Using this method, the saccharide content of samples of carrot (Daucus carota L.) roots was compared to the results given from similar samples by the formerly published q 1H NMR determination of extracts obtained by the O'Donoghue/Davis method. The content in glucose and sucrose is significantly higher with the direct method than when an extraction step is included; the content in fructose is not significantly different. If a possible detection of saccharides included in glycosylated biological compounds is to be excluded, a more complete extraction of saccharides is probably obtained using the direct method. | ||
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10.1007/s00216-010-4311-6 doi (DE-627)SPR002195429 (SPR)s00216-010-4311-6-e DE-627 ger DE-627 rakwb eng Weberskirch, Linda verfasserin aut Comparison of two liquid-state NMR methods for the determination of saccharides in carrot (Daucus carota L.) roots 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag 2010 Abstract In order to determine the saccharide content of plant tissues, we studied a new non-destructive and fast analytical method that we call “direct quantitative proton nuclear magnetic resonance spectroscopy” (d q 1H NMR): the application of quantitative proton nuclear magnetic resonance spectroscopy (q 1H NMR) to non modified plant tissues along with capillary tubes containing a reference compound (for quantification) and deuterium oxide (for locking). Using this method, the saccharide content of samples of carrot (Daucus carota L.) roots was compared to the results given from similar samples by the formerly published q 1H NMR determination of extracts obtained by the O'Donoghue/Davis method. The content in glucose and sucrose is significantly higher with the direct method than when an extraction step is included; the content in fructose is not significantly different. If a possible detection of saccharides included in glycosylated biological compounds is to be excluded, a more complete extraction of saccharides is probably obtained using the direct method. NMR/ESR (dpeaa)DE-He213 Foods/beverages (dpeaa)DE-He213 Natural products (dpeaa)DE-He213 Organic compounds/trace organic compounds (dpeaa)DE-He213 Luna, Alan aut Skoglund, Sara aut This, Hervé aut Enthalten in Analytical and bioanalytical chemistry Berlin : Springer, 2002 399(2010), 1 vom: 28. Okt., Seite 483-487 (DE-627)25372337X (DE-600)1459122-4 1618-2650 nnns volume:399 year:2010 number:1 day:28 month:10 pages:483-487 https://dx.doi.org/10.1007/s00216-010-4311-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2360 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 399 2010 1 28 10 483-487 |
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10.1007/s00216-010-4311-6 doi (DE-627)SPR002195429 (SPR)s00216-010-4311-6-e DE-627 ger DE-627 rakwb eng Weberskirch, Linda verfasserin aut Comparison of two liquid-state NMR methods for the determination of saccharides in carrot (Daucus carota L.) roots 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag 2010 Abstract In order to determine the saccharide content of plant tissues, we studied a new non-destructive and fast analytical method that we call “direct quantitative proton nuclear magnetic resonance spectroscopy” (d q 1H NMR): the application of quantitative proton nuclear magnetic resonance spectroscopy (q 1H NMR) to non modified plant tissues along with capillary tubes containing a reference compound (for quantification) and deuterium oxide (for locking). Using this method, the saccharide content of samples of carrot (Daucus carota L.) roots was compared to the results given from similar samples by the formerly published q 1H NMR determination of extracts obtained by the O'Donoghue/Davis method. The content in glucose and sucrose is significantly higher with the direct method than when an extraction step is included; the content in fructose is not significantly different. If a possible detection of saccharides included in glycosylated biological compounds is to be excluded, a more complete extraction of saccharides is probably obtained using the direct method. NMR/ESR (dpeaa)DE-He213 Foods/beverages (dpeaa)DE-He213 Natural products (dpeaa)DE-He213 Organic compounds/trace organic compounds (dpeaa)DE-He213 Luna, Alan aut Skoglund, Sara aut This, Hervé aut Enthalten in Analytical and bioanalytical chemistry Berlin : Springer, 2002 399(2010), 1 vom: 28. Okt., Seite 483-487 (DE-627)25372337X (DE-600)1459122-4 1618-2650 nnns volume:399 year:2010 number:1 day:28 month:10 pages:483-487 https://dx.doi.org/10.1007/s00216-010-4311-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2360 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 399 2010 1 28 10 483-487 |
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10.1007/s00216-010-4311-6 doi (DE-627)SPR002195429 (SPR)s00216-010-4311-6-e DE-627 ger DE-627 rakwb eng Weberskirch, Linda verfasserin aut Comparison of two liquid-state NMR methods for the determination of saccharides in carrot (Daucus carota L.) roots 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag 2010 Abstract In order to determine the saccharide content of plant tissues, we studied a new non-destructive and fast analytical method that we call “direct quantitative proton nuclear magnetic resonance spectroscopy” (d q 1H NMR): the application of quantitative proton nuclear magnetic resonance spectroscopy (q 1H NMR) to non modified plant tissues along with capillary tubes containing a reference compound (for quantification) and deuterium oxide (for locking). Using this method, the saccharide content of samples of carrot (Daucus carota L.) roots was compared to the results given from similar samples by the formerly published q 1H NMR determination of extracts obtained by the O'Donoghue/Davis method. The content in glucose and sucrose is significantly higher with the direct method than when an extraction step is included; the content in fructose is not significantly different. If a possible detection of saccharides included in glycosylated biological compounds is to be excluded, a more complete extraction of saccharides is probably obtained using the direct method. NMR/ESR (dpeaa)DE-He213 Foods/beverages (dpeaa)DE-He213 Natural products (dpeaa)DE-He213 Organic compounds/trace organic compounds (dpeaa)DE-He213 Luna, Alan aut Skoglund, Sara aut This, Hervé aut Enthalten in Analytical and bioanalytical chemistry Berlin : Springer, 2002 399(2010), 1 vom: 28. Okt., Seite 483-487 (DE-627)25372337X (DE-600)1459122-4 1618-2650 nnns volume:399 year:2010 number:1 day:28 month:10 pages:483-487 https://dx.doi.org/10.1007/s00216-010-4311-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2360 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 399 2010 1 28 10 483-487 |
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10.1007/s00216-010-4311-6 doi (DE-627)SPR002195429 (SPR)s00216-010-4311-6-e DE-627 ger DE-627 rakwb eng Weberskirch, Linda verfasserin aut Comparison of two liquid-state NMR methods for the determination of saccharides in carrot (Daucus carota L.) roots 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag 2010 Abstract In order to determine the saccharide content of plant tissues, we studied a new non-destructive and fast analytical method that we call “direct quantitative proton nuclear magnetic resonance spectroscopy” (d q 1H NMR): the application of quantitative proton nuclear magnetic resonance spectroscopy (q 1H NMR) to non modified plant tissues along with capillary tubes containing a reference compound (for quantification) and deuterium oxide (for locking). Using this method, the saccharide content of samples of carrot (Daucus carota L.) roots was compared to the results given from similar samples by the formerly published q 1H NMR determination of extracts obtained by the O'Donoghue/Davis method. The content in glucose and sucrose is significantly higher with the direct method than when an extraction step is included; the content in fructose is not significantly different. If a possible detection of saccharides included in glycosylated biological compounds is to be excluded, a more complete extraction of saccharides is probably obtained using the direct method. NMR/ESR (dpeaa)DE-He213 Foods/beverages (dpeaa)DE-He213 Natural products (dpeaa)DE-He213 Organic compounds/trace organic compounds (dpeaa)DE-He213 Luna, Alan aut Skoglund, Sara aut This, Hervé aut Enthalten in Analytical and bioanalytical chemistry Berlin : Springer, 2002 399(2010), 1 vom: 28. Okt., Seite 483-487 (DE-627)25372337X (DE-600)1459122-4 1618-2650 nnns volume:399 year:2010 number:1 day:28 month:10 pages:483-487 https://dx.doi.org/10.1007/s00216-010-4311-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2360 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 399 2010 1 28 10 483-487 |
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10.1007/s00216-010-4311-6 doi (DE-627)SPR002195429 (SPR)s00216-010-4311-6-e DE-627 ger DE-627 rakwb eng Weberskirch, Linda verfasserin aut Comparison of two liquid-state NMR methods for the determination of saccharides in carrot (Daucus carota L.) roots 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag 2010 Abstract In order to determine the saccharide content of plant tissues, we studied a new non-destructive and fast analytical method that we call “direct quantitative proton nuclear magnetic resonance spectroscopy” (d q 1H NMR): the application of quantitative proton nuclear magnetic resonance spectroscopy (q 1H NMR) to non modified plant tissues along with capillary tubes containing a reference compound (for quantification) and deuterium oxide (for locking). Using this method, the saccharide content of samples of carrot (Daucus carota L.) roots was compared to the results given from similar samples by the formerly published q 1H NMR determination of extracts obtained by the O'Donoghue/Davis method. The content in glucose and sucrose is significantly higher with the direct method than when an extraction step is included; the content in fructose is not significantly different. If a possible detection of saccharides included in glycosylated biological compounds is to be excluded, a more complete extraction of saccharides is probably obtained using the direct method. NMR/ESR (dpeaa)DE-He213 Foods/beverages (dpeaa)DE-He213 Natural products (dpeaa)DE-He213 Organic compounds/trace organic compounds (dpeaa)DE-He213 Luna, Alan aut Skoglund, Sara aut This, Hervé aut Enthalten in Analytical and bioanalytical chemistry Berlin : Springer, 2002 399(2010), 1 vom: 28. Okt., Seite 483-487 (DE-627)25372337X (DE-600)1459122-4 1618-2650 nnns volume:399 year:2010 number:1 day:28 month:10 pages:483-487 https://dx.doi.org/10.1007/s00216-010-4311-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2360 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 399 2010 1 28 10 483-487 |
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Weberskirch, Linda @@aut@@ Luna, Alan @@aut@@ Skoglund, Sara @@aut@@ This, Hervé @@aut@@ |
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Weberskirch, Linda |
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Weberskirch, Linda misc NMR/ESR misc Foods/beverages misc Natural products misc Organic compounds/trace organic compounds Comparison of two liquid-state NMR methods for the determination of saccharides in carrot (Daucus carota L.) roots |
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Comparison of two liquid-state NMR methods for the determination of saccharides in carrot (Daucus carota L.) roots NMR/ESR (dpeaa)DE-He213 Foods/beverages (dpeaa)DE-He213 Natural products (dpeaa)DE-He213 Organic compounds/trace organic compounds (dpeaa)DE-He213 |
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Comparison of two liquid-state NMR methods for the determination of saccharides in carrot (Daucus carota L.) roots |
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Comparison of two liquid-state NMR methods for the determination of saccharides in carrot (Daucus carota L.) roots |
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comparison of two liquid-state nmr methods for the determination of saccharides in carrot (daucus carota l.) roots |
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Comparison of two liquid-state NMR methods for the determination of saccharides in carrot (Daucus carota L.) roots |
| abstract |
Abstract In order to determine the saccharide content of plant tissues, we studied a new non-destructive and fast analytical method that we call “direct quantitative proton nuclear magnetic resonance spectroscopy” (d q 1H NMR): the application of quantitative proton nuclear magnetic resonance spectroscopy (q 1H NMR) to non modified plant tissues along with capillary tubes containing a reference compound (for quantification) and deuterium oxide (for locking). Using this method, the saccharide content of samples of carrot (Daucus carota L.) roots was compared to the results given from similar samples by the formerly published q 1H NMR determination of extracts obtained by the O'Donoghue/Davis method. The content in glucose and sucrose is significantly higher with the direct method than when an extraction step is included; the content in fructose is not significantly different. If a possible detection of saccharides included in glycosylated biological compounds is to be excluded, a more complete extraction of saccharides is probably obtained using the direct method. © Springer-Verlag 2010 |
| abstractGer |
Abstract In order to determine the saccharide content of plant tissues, we studied a new non-destructive and fast analytical method that we call “direct quantitative proton nuclear magnetic resonance spectroscopy” (d q 1H NMR): the application of quantitative proton nuclear magnetic resonance spectroscopy (q 1H NMR) to non modified plant tissues along with capillary tubes containing a reference compound (for quantification) and deuterium oxide (for locking). Using this method, the saccharide content of samples of carrot (Daucus carota L.) roots was compared to the results given from similar samples by the formerly published q 1H NMR determination of extracts obtained by the O'Donoghue/Davis method. The content in glucose and sucrose is significantly higher with the direct method than when an extraction step is included; the content in fructose is not significantly different. If a possible detection of saccharides included in glycosylated biological compounds is to be excluded, a more complete extraction of saccharides is probably obtained using the direct method. © Springer-Verlag 2010 |
| abstract_unstemmed |
Abstract In order to determine the saccharide content of plant tissues, we studied a new non-destructive and fast analytical method that we call “direct quantitative proton nuclear magnetic resonance spectroscopy” (d q 1H NMR): the application of quantitative proton nuclear magnetic resonance spectroscopy (q 1H NMR) to non modified plant tissues along with capillary tubes containing a reference compound (for quantification) and deuterium oxide (for locking). Using this method, the saccharide content of samples of carrot (Daucus carota L.) roots was compared to the results given from similar samples by the formerly published q 1H NMR determination of extracts obtained by the O'Donoghue/Davis method. The content in glucose and sucrose is significantly higher with the direct method than when an extraction step is included; the content in fructose is not significantly different. If a possible detection of saccharides included in glycosylated biological compounds is to be excluded, a more complete extraction of saccharides is probably obtained using the direct method. © Springer-Verlag 2010 |
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Comparison of two liquid-state NMR methods for the determination of saccharides in carrot (Daucus carota L.) roots |
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https://dx.doi.org/10.1007/s00216-010-4311-6 |
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Luna, Alan Skoglund, Sara This, Hervé |
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| score |
7.403078 |