Analytical method for the accurate determination of tricothecenes in grains using LC-MS/MS: a comparison between MRM transition and $ MS^{3} $ quantitation
Abstract The current food crisis demands unambiguous determination of mycotoxin contamination in staple foods to achieve safer food for consumption. This paper describes the first accurate LC-MS/MS method developed to analyze tricothecenes in grains by applying multiple reaction monitoring (MRM) tra...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Lim, Chee Wei [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2011 |
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| Schlagwörter: |
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| Anmerkung: |
© Springer-Verlag 2011 |
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| Übergeordnetes Werk: |
Enthalten in: Analytical and bioanalytical chemistry - Berlin : Springer, 2002, 403(2011), 10 vom: 31. Dez., Seite 2801-2806 |
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| Übergeordnetes Werk: |
volume:403 ; year:2011 ; number:10 ; day:31 ; month:12 ; pages:2801-2806 |
| Links: |
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| DOI / URN: |
10.1007/s00216-011-5558-2 |
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| Katalog-ID: |
SPR002209837 |
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| 100 | 1 | |a Lim, Chee Wei |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Analytical method for the accurate determination of tricothecenes in grains using LC-MS/MS: a comparison between MRM transition and $ MS^{3} $ quantitation |
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| 520 | |a Abstract The current food crisis demands unambiguous determination of mycotoxin contamination in staple foods to achieve safer food for consumption. This paper describes the first accurate LC-MS/MS method developed to analyze tricothecenes in grains by applying multiple reaction monitoring (MRM) transition and $ MS^{3} $ quantitation strategies in tandem. The tricothecenes are nivalenol, deoxynivalenol, deoxynivalenol-3-glucoside, fusarenon X, 3-acetyl-deoxynivalenol, 15-acetyldeoxynivalenol, diacetoxyscirpenol, and HT-2 and T-2 toxins. Acetic acid and ammonium acetate were used to convert the analytes into their respective acetate adducts and ammonium adducts under negative and positive MS polarity conditions, respectively. The mycotoxins were separated by reversed-phase LC in a 13.5-min run, ionized using electrospray ionization, and detected by tandem mass spectrometry. Analyte-specific mass-to-charge (m/z) ratios were used to perform quantitation under MRM transition and $ MS^{3} $ (linear ion trap) modes. Three experiments were made for each quantitation mode and matrix in batches over 6 days for recovery studies. The matrix effect was investigated at concentration levels of 20, 40, 80, 120, 160, and 200 μg $ kg^{−1} $ (n = 3) in 5 g corn flour and rice flour. Extraction with acetonitrile provided a good overall recovery range of 90–108% (n = 3) at three levels of spiking concentration of 40, 80, and 120 μg $ kg^{−1} $. A quantitation limit of 2–6 μg $ kg^{−1} $ was achieved by applying an MRM transition quantitation strategy. Under $ MS^{3} $ mode, a quantitation limit of 4–10 μg $ kg^{−1} $ was achieved. Relative standard deviations of 2–10% and 2–11% were reported for MRM transition and $ MS^{3} $ quantitation, respectively. The successful utilization of $ MS^{3} $ enabled accurate analyte fragmentation pattern matching and its quantitation, leading to the development of analytical methods in fields that demand both analyte specificity and fragmentation fingerprint-matching capabilities that are unavailable under MRM transition. FigureThe power of QTRAP® technology | ||
| 650 | 4 | |a LC-MS/MS |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Grains |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a MRM transition |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a MS/MS/MS (MS |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a ) |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Quantitation |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Tricothecenes |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Tai, Siew Hoon |4 aut | |
| 700 | 1 | |a Lee, Lin Min |4 aut | |
| 700 | 1 | |a Chan, Sheot Harn |4 aut | |
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10.1007/s00216-011-5558-2 doi (DE-627)SPR002209837 (SPR)s00216-011-5558-2-e DE-627 ger DE-627 rakwb eng Lim, Chee Wei verfasserin aut Analytical method for the accurate determination of tricothecenes in grains using LC-MS/MS: a comparison between MRM transition and $ MS^{3} $ quantitation 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag 2011 Abstract The current food crisis demands unambiguous determination of mycotoxin contamination in staple foods to achieve safer food for consumption. This paper describes the first accurate LC-MS/MS method developed to analyze tricothecenes in grains by applying multiple reaction monitoring (MRM) transition and $ MS^{3} $ quantitation strategies in tandem. The tricothecenes are nivalenol, deoxynivalenol, deoxynivalenol-3-glucoside, fusarenon X, 3-acetyl-deoxynivalenol, 15-acetyldeoxynivalenol, diacetoxyscirpenol, and HT-2 and T-2 toxins. Acetic acid and ammonium acetate were used to convert the analytes into their respective acetate adducts and ammonium adducts under negative and positive MS polarity conditions, respectively. The mycotoxins were separated by reversed-phase LC in a 13.5-min run, ionized using electrospray ionization, and detected by tandem mass spectrometry. Analyte-specific mass-to-charge (m/z) ratios were used to perform quantitation under MRM transition and $ MS^{3} $ (linear ion trap) modes. Three experiments were made for each quantitation mode and matrix in batches over 6 days for recovery studies. The matrix effect was investigated at concentration levels of 20, 40, 80, 120, 160, and 200 μg $ kg^{−1} $ (n = 3) in 5 g corn flour and rice flour. Extraction with acetonitrile provided a good overall recovery range of 90–108% (n = 3) at three levels of spiking concentration of 40, 80, and 120 μg $ kg^{−1} $. A quantitation limit of 2–6 μg $ kg^{−1} $ was achieved by applying an MRM transition quantitation strategy. Under $ MS^{3} $ mode, a quantitation limit of 4–10 μg $ kg^{−1} $ was achieved. Relative standard deviations of 2–10% and 2–11% were reported for MRM transition and $ MS^{3} $ quantitation, respectively. The successful utilization of $ MS^{3} $ enabled accurate analyte fragmentation pattern matching and its quantitation, leading to the development of analytical methods in fields that demand both analyte specificity and fragmentation fingerprint-matching capabilities that are unavailable under MRM transition. FigureThe power of QTRAP® technology LC-MS/MS (dpeaa)DE-He213 Grains (dpeaa)DE-He213 MRM transition (dpeaa)DE-He213 MS/MS/MS (MS (dpeaa)DE-He213 ) (dpeaa)DE-He213 Quantitation (dpeaa)DE-He213 Tricothecenes (dpeaa)DE-He213 Tai, Siew Hoon aut Lee, Lin Min aut Chan, Sheot Harn aut Enthalten in Analytical and bioanalytical chemistry Berlin : Springer, 2002 403(2011), 10 vom: 31. Dez., Seite 2801-2806 (DE-627)25372337X (DE-600)1459122-4 1618-2650 nnns volume:403 year:2011 number:10 day:31 month:12 pages:2801-2806 https://dx.doi.org/10.1007/s00216-011-5558-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2360 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 403 2011 10 31 12 2801-2806 |
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10.1007/s00216-011-5558-2 doi (DE-627)SPR002209837 (SPR)s00216-011-5558-2-e DE-627 ger DE-627 rakwb eng Lim, Chee Wei verfasserin aut Analytical method for the accurate determination of tricothecenes in grains using LC-MS/MS: a comparison between MRM transition and $ MS^{3} $ quantitation 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag 2011 Abstract The current food crisis demands unambiguous determination of mycotoxin contamination in staple foods to achieve safer food for consumption. This paper describes the first accurate LC-MS/MS method developed to analyze tricothecenes in grains by applying multiple reaction monitoring (MRM) transition and $ MS^{3} $ quantitation strategies in tandem. The tricothecenes are nivalenol, deoxynivalenol, deoxynivalenol-3-glucoside, fusarenon X, 3-acetyl-deoxynivalenol, 15-acetyldeoxynivalenol, diacetoxyscirpenol, and HT-2 and T-2 toxins. Acetic acid and ammonium acetate were used to convert the analytes into their respective acetate adducts and ammonium adducts under negative and positive MS polarity conditions, respectively. The mycotoxins were separated by reversed-phase LC in a 13.5-min run, ionized using electrospray ionization, and detected by tandem mass spectrometry. Analyte-specific mass-to-charge (m/z) ratios were used to perform quantitation under MRM transition and $ MS^{3} $ (linear ion trap) modes. Three experiments were made for each quantitation mode and matrix in batches over 6 days for recovery studies. The matrix effect was investigated at concentration levels of 20, 40, 80, 120, 160, and 200 μg $ kg^{−1} $ (n = 3) in 5 g corn flour and rice flour. Extraction with acetonitrile provided a good overall recovery range of 90–108% (n = 3) at three levels of spiking concentration of 40, 80, and 120 μg $ kg^{−1} $. A quantitation limit of 2–6 μg $ kg^{−1} $ was achieved by applying an MRM transition quantitation strategy. Under $ MS^{3} $ mode, a quantitation limit of 4–10 μg $ kg^{−1} $ was achieved. Relative standard deviations of 2–10% and 2–11% were reported for MRM transition and $ MS^{3} $ quantitation, respectively. The successful utilization of $ MS^{3} $ enabled accurate analyte fragmentation pattern matching and its quantitation, leading to the development of analytical methods in fields that demand both analyte specificity and fragmentation fingerprint-matching capabilities that are unavailable under MRM transition. FigureThe power of QTRAP® technology LC-MS/MS (dpeaa)DE-He213 Grains (dpeaa)DE-He213 MRM transition (dpeaa)DE-He213 MS/MS/MS (MS (dpeaa)DE-He213 ) (dpeaa)DE-He213 Quantitation (dpeaa)DE-He213 Tricothecenes (dpeaa)DE-He213 Tai, Siew Hoon aut Lee, Lin Min aut Chan, Sheot Harn aut Enthalten in Analytical and bioanalytical chemistry Berlin : Springer, 2002 403(2011), 10 vom: 31. Dez., Seite 2801-2806 (DE-627)25372337X (DE-600)1459122-4 1618-2650 nnns volume:403 year:2011 number:10 day:31 month:12 pages:2801-2806 https://dx.doi.org/10.1007/s00216-011-5558-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2360 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 403 2011 10 31 12 2801-2806 |
| allfields_unstemmed |
10.1007/s00216-011-5558-2 doi (DE-627)SPR002209837 (SPR)s00216-011-5558-2-e DE-627 ger DE-627 rakwb eng Lim, Chee Wei verfasserin aut Analytical method for the accurate determination of tricothecenes in grains using LC-MS/MS: a comparison between MRM transition and $ MS^{3} $ quantitation 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag 2011 Abstract The current food crisis demands unambiguous determination of mycotoxin contamination in staple foods to achieve safer food for consumption. This paper describes the first accurate LC-MS/MS method developed to analyze tricothecenes in grains by applying multiple reaction monitoring (MRM) transition and $ MS^{3} $ quantitation strategies in tandem. The tricothecenes are nivalenol, deoxynivalenol, deoxynivalenol-3-glucoside, fusarenon X, 3-acetyl-deoxynivalenol, 15-acetyldeoxynivalenol, diacetoxyscirpenol, and HT-2 and T-2 toxins. Acetic acid and ammonium acetate were used to convert the analytes into their respective acetate adducts and ammonium adducts under negative and positive MS polarity conditions, respectively. The mycotoxins were separated by reversed-phase LC in a 13.5-min run, ionized using electrospray ionization, and detected by tandem mass spectrometry. Analyte-specific mass-to-charge (m/z) ratios were used to perform quantitation under MRM transition and $ MS^{3} $ (linear ion trap) modes. Three experiments were made for each quantitation mode and matrix in batches over 6 days for recovery studies. The matrix effect was investigated at concentration levels of 20, 40, 80, 120, 160, and 200 μg $ kg^{−1} $ (n = 3) in 5 g corn flour and rice flour. Extraction with acetonitrile provided a good overall recovery range of 90–108% (n = 3) at three levels of spiking concentration of 40, 80, and 120 μg $ kg^{−1} $. A quantitation limit of 2–6 μg $ kg^{−1} $ was achieved by applying an MRM transition quantitation strategy. Under $ MS^{3} $ mode, a quantitation limit of 4–10 μg $ kg^{−1} $ was achieved. Relative standard deviations of 2–10% and 2–11% were reported for MRM transition and $ MS^{3} $ quantitation, respectively. The successful utilization of $ MS^{3} $ enabled accurate analyte fragmentation pattern matching and its quantitation, leading to the development of analytical methods in fields that demand both analyte specificity and fragmentation fingerprint-matching capabilities that are unavailable under MRM transition. FigureThe power of QTRAP® technology LC-MS/MS (dpeaa)DE-He213 Grains (dpeaa)DE-He213 MRM transition (dpeaa)DE-He213 MS/MS/MS (MS (dpeaa)DE-He213 ) (dpeaa)DE-He213 Quantitation (dpeaa)DE-He213 Tricothecenes (dpeaa)DE-He213 Tai, Siew Hoon aut Lee, Lin Min aut Chan, Sheot Harn aut Enthalten in Analytical and bioanalytical chemistry Berlin : Springer, 2002 403(2011), 10 vom: 31. Dez., Seite 2801-2806 (DE-627)25372337X (DE-600)1459122-4 1618-2650 nnns volume:403 year:2011 number:10 day:31 month:12 pages:2801-2806 https://dx.doi.org/10.1007/s00216-011-5558-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2360 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 403 2011 10 31 12 2801-2806 |
| allfieldsGer |
10.1007/s00216-011-5558-2 doi (DE-627)SPR002209837 (SPR)s00216-011-5558-2-e DE-627 ger DE-627 rakwb eng Lim, Chee Wei verfasserin aut Analytical method for the accurate determination of tricothecenes in grains using LC-MS/MS: a comparison between MRM transition and $ MS^{3} $ quantitation 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag 2011 Abstract The current food crisis demands unambiguous determination of mycotoxin contamination in staple foods to achieve safer food for consumption. This paper describes the first accurate LC-MS/MS method developed to analyze tricothecenes in grains by applying multiple reaction monitoring (MRM) transition and $ MS^{3} $ quantitation strategies in tandem. The tricothecenes are nivalenol, deoxynivalenol, deoxynivalenol-3-glucoside, fusarenon X, 3-acetyl-deoxynivalenol, 15-acetyldeoxynivalenol, diacetoxyscirpenol, and HT-2 and T-2 toxins. Acetic acid and ammonium acetate were used to convert the analytes into their respective acetate adducts and ammonium adducts under negative and positive MS polarity conditions, respectively. The mycotoxins were separated by reversed-phase LC in a 13.5-min run, ionized using electrospray ionization, and detected by tandem mass spectrometry. Analyte-specific mass-to-charge (m/z) ratios were used to perform quantitation under MRM transition and $ MS^{3} $ (linear ion trap) modes. Three experiments were made for each quantitation mode and matrix in batches over 6 days for recovery studies. The matrix effect was investigated at concentration levels of 20, 40, 80, 120, 160, and 200 μg $ kg^{−1} $ (n = 3) in 5 g corn flour and rice flour. Extraction with acetonitrile provided a good overall recovery range of 90–108% (n = 3) at three levels of spiking concentration of 40, 80, and 120 μg $ kg^{−1} $. A quantitation limit of 2–6 μg $ kg^{−1} $ was achieved by applying an MRM transition quantitation strategy. Under $ MS^{3} $ mode, a quantitation limit of 4–10 μg $ kg^{−1} $ was achieved. Relative standard deviations of 2–10% and 2–11% were reported for MRM transition and $ MS^{3} $ quantitation, respectively. The successful utilization of $ MS^{3} $ enabled accurate analyte fragmentation pattern matching and its quantitation, leading to the development of analytical methods in fields that demand both analyte specificity and fragmentation fingerprint-matching capabilities that are unavailable under MRM transition. FigureThe power of QTRAP® technology LC-MS/MS (dpeaa)DE-He213 Grains (dpeaa)DE-He213 MRM transition (dpeaa)DE-He213 MS/MS/MS (MS (dpeaa)DE-He213 ) (dpeaa)DE-He213 Quantitation (dpeaa)DE-He213 Tricothecenes (dpeaa)DE-He213 Tai, Siew Hoon aut Lee, Lin Min aut Chan, Sheot Harn aut Enthalten in Analytical and bioanalytical chemistry Berlin : Springer, 2002 403(2011), 10 vom: 31. Dez., Seite 2801-2806 (DE-627)25372337X (DE-600)1459122-4 1618-2650 nnns volume:403 year:2011 number:10 day:31 month:12 pages:2801-2806 https://dx.doi.org/10.1007/s00216-011-5558-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2360 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 403 2011 10 31 12 2801-2806 |
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10.1007/s00216-011-5558-2 doi (DE-627)SPR002209837 (SPR)s00216-011-5558-2-e DE-627 ger DE-627 rakwb eng Lim, Chee Wei verfasserin aut Analytical method for the accurate determination of tricothecenes in grains using LC-MS/MS: a comparison between MRM transition and $ MS^{3} $ quantitation 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag 2011 Abstract The current food crisis demands unambiguous determination of mycotoxin contamination in staple foods to achieve safer food for consumption. This paper describes the first accurate LC-MS/MS method developed to analyze tricothecenes in grains by applying multiple reaction monitoring (MRM) transition and $ MS^{3} $ quantitation strategies in tandem. The tricothecenes are nivalenol, deoxynivalenol, deoxynivalenol-3-glucoside, fusarenon X, 3-acetyl-deoxynivalenol, 15-acetyldeoxynivalenol, diacetoxyscirpenol, and HT-2 and T-2 toxins. Acetic acid and ammonium acetate were used to convert the analytes into their respective acetate adducts and ammonium adducts under negative and positive MS polarity conditions, respectively. The mycotoxins were separated by reversed-phase LC in a 13.5-min run, ionized using electrospray ionization, and detected by tandem mass spectrometry. Analyte-specific mass-to-charge (m/z) ratios were used to perform quantitation under MRM transition and $ MS^{3} $ (linear ion trap) modes. Three experiments were made for each quantitation mode and matrix in batches over 6 days for recovery studies. The matrix effect was investigated at concentration levels of 20, 40, 80, 120, 160, and 200 μg $ kg^{−1} $ (n = 3) in 5 g corn flour and rice flour. Extraction with acetonitrile provided a good overall recovery range of 90–108% (n = 3) at three levels of spiking concentration of 40, 80, and 120 μg $ kg^{−1} $. A quantitation limit of 2–6 μg $ kg^{−1} $ was achieved by applying an MRM transition quantitation strategy. Under $ MS^{3} $ mode, a quantitation limit of 4–10 μg $ kg^{−1} $ was achieved. Relative standard deviations of 2–10% and 2–11% were reported for MRM transition and $ MS^{3} $ quantitation, respectively. The successful utilization of $ MS^{3} $ enabled accurate analyte fragmentation pattern matching and its quantitation, leading to the development of analytical methods in fields that demand both analyte specificity and fragmentation fingerprint-matching capabilities that are unavailable under MRM transition. FigureThe power of QTRAP® technology LC-MS/MS (dpeaa)DE-He213 Grains (dpeaa)DE-He213 MRM transition (dpeaa)DE-He213 MS/MS/MS (MS (dpeaa)DE-He213 ) (dpeaa)DE-He213 Quantitation (dpeaa)DE-He213 Tricothecenes (dpeaa)DE-He213 Tai, Siew Hoon aut Lee, Lin Min aut Chan, Sheot Harn aut Enthalten in Analytical and bioanalytical chemistry Berlin : Springer, 2002 403(2011), 10 vom: 31. Dez., Seite 2801-2806 (DE-627)25372337X (DE-600)1459122-4 1618-2650 nnns volume:403 year:2011 number:10 day:31 month:12 pages:2801-2806 https://dx.doi.org/10.1007/s00216-011-5558-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2360 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 403 2011 10 31 12 2801-2806 |
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Enthalten in Analytical and bioanalytical chemistry 403(2011), 10 vom: 31. Dez., Seite 2801-2806 volume:403 year:2011 number:10 day:31 month:12 pages:2801-2806 |
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Lim, Chee Wei @@aut@@ Tai, Siew Hoon @@aut@@ Lee, Lin Min @@aut@@ Chan, Sheot Harn @@aut@@ |
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2011-12-31T00:00:00Z |
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| author |
Lim, Chee Wei |
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Lim, Chee Wei misc LC-MS/MS misc Grains misc MRM transition misc MS/MS/MS (MS misc ) misc Quantitation misc Tricothecenes Analytical method for the accurate determination of tricothecenes in grains using LC-MS/MS: a comparison between MRM transition and $ MS^{3} $ quantitation |
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Analytical method for the accurate determination of tricothecenes in grains using LC-MS/MS: a comparison between MRM transition and $ MS^{3} $ quantitation LC-MS/MS (dpeaa)DE-He213 Grains (dpeaa)DE-He213 MRM transition (dpeaa)DE-He213 MS/MS/MS (MS (dpeaa)DE-He213 ) (dpeaa)DE-He213 Quantitation (dpeaa)DE-He213 Tricothecenes (dpeaa)DE-He213 |
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misc LC-MS/MS misc Grains misc MRM transition misc MS/MS/MS (MS misc ) misc Quantitation misc Tricothecenes |
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Analytical method for the accurate determination of tricothecenes in grains using LC-MS/MS: a comparison between MRM transition and $ MS^{3} $ quantitation |
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Analytical method for the accurate determination of tricothecenes in grains using LC-MS/MS: a comparison between MRM transition and $ MS^{3} $ quantitation |
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Lim, Chee Wei |
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Lim, Chee Wei Tai, Siew Hoon Lee, Lin Min Chan, Sheot Harn |
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Lim, Chee Wei |
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10.1007/s00216-011-5558-2 |
| title_sort |
analytical method for the accurate determination of tricothecenes in grains using lc-ms/ms: a comparison between mrm transition and $ ms^{3} $ quantitation |
| title_auth |
Analytical method for the accurate determination of tricothecenes in grains using LC-MS/MS: a comparison between MRM transition and $ MS^{3} $ quantitation |
| abstract |
Abstract The current food crisis demands unambiguous determination of mycotoxin contamination in staple foods to achieve safer food for consumption. This paper describes the first accurate LC-MS/MS method developed to analyze tricothecenes in grains by applying multiple reaction monitoring (MRM) transition and $ MS^{3} $ quantitation strategies in tandem. The tricothecenes are nivalenol, deoxynivalenol, deoxynivalenol-3-glucoside, fusarenon X, 3-acetyl-deoxynivalenol, 15-acetyldeoxynivalenol, diacetoxyscirpenol, and HT-2 and T-2 toxins. Acetic acid and ammonium acetate were used to convert the analytes into their respective acetate adducts and ammonium adducts under negative and positive MS polarity conditions, respectively. The mycotoxins were separated by reversed-phase LC in a 13.5-min run, ionized using electrospray ionization, and detected by tandem mass spectrometry. Analyte-specific mass-to-charge (m/z) ratios were used to perform quantitation under MRM transition and $ MS^{3} $ (linear ion trap) modes. Three experiments were made for each quantitation mode and matrix in batches over 6 days for recovery studies. The matrix effect was investigated at concentration levels of 20, 40, 80, 120, 160, and 200 μg $ kg^{−1} $ (n = 3) in 5 g corn flour and rice flour. Extraction with acetonitrile provided a good overall recovery range of 90–108% (n = 3) at three levels of spiking concentration of 40, 80, and 120 μg $ kg^{−1} $. A quantitation limit of 2–6 μg $ kg^{−1} $ was achieved by applying an MRM transition quantitation strategy. Under $ MS^{3} $ mode, a quantitation limit of 4–10 μg $ kg^{−1} $ was achieved. Relative standard deviations of 2–10% and 2–11% were reported for MRM transition and $ MS^{3} $ quantitation, respectively. The successful utilization of $ MS^{3} $ enabled accurate analyte fragmentation pattern matching and its quantitation, leading to the development of analytical methods in fields that demand both analyte specificity and fragmentation fingerprint-matching capabilities that are unavailable under MRM transition. FigureThe power of QTRAP® technology © Springer-Verlag 2011 |
| abstractGer |
Abstract The current food crisis demands unambiguous determination of mycotoxin contamination in staple foods to achieve safer food for consumption. This paper describes the first accurate LC-MS/MS method developed to analyze tricothecenes in grains by applying multiple reaction monitoring (MRM) transition and $ MS^{3} $ quantitation strategies in tandem. The tricothecenes are nivalenol, deoxynivalenol, deoxynivalenol-3-glucoside, fusarenon X, 3-acetyl-deoxynivalenol, 15-acetyldeoxynivalenol, diacetoxyscirpenol, and HT-2 and T-2 toxins. Acetic acid and ammonium acetate were used to convert the analytes into their respective acetate adducts and ammonium adducts under negative and positive MS polarity conditions, respectively. The mycotoxins were separated by reversed-phase LC in a 13.5-min run, ionized using electrospray ionization, and detected by tandem mass spectrometry. Analyte-specific mass-to-charge (m/z) ratios were used to perform quantitation under MRM transition and $ MS^{3} $ (linear ion trap) modes. Three experiments were made for each quantitation mode and matrix in batches over 6 days for recovery studies. The matrix effect was investigated at concentration levels of 20, 40, 80, 120, 160, and 200 μg $ kg^{−1} $ (n = 3) in 5 g corn flour and rice flour. Extraction with acetonitrile provided a good overall recovery range of 90–108% (n = 3) at three levels of spiking concentration of 40, 80, and 120 μg $ kg^{−1} $. A quantitation limit of 2–6 μg $ kg^{−1} $ was achieved by applying an MRM transition quantitation strategy. Under $ MS^{3} $ mode, a quantitation limit of 4–10 μg $ kg^{−1} $ was achieved. Relative standard deviations of 2–10% and 2–11% were reported for MRM transition and $ MS^{3} $ quantitation, respectively. The successful utilization of $ MS^{3} $ enabled accurate analyte fragmentation pattern matching and its quantitation, leading to the development of analytical methods in fields that demand both analyte specificity and fragmentation fingerprint-matching capabilities that are unavailable under MRM transition. FigureThe power of QTRAP® technology © Springer-Verlag 2011 |
| abstract_unstemmed |
Abstract The current food crisis demands unambiguous determination of mycotoxin contamination in staple foods to achieve safer food for consumption. This paper describes the first accurate LC-MS/MS method developed to analyze tricothecenes in grains by applying multiple reaction monitoring (MRM) transition and $ MS^{3} $ quantitation strategies in tandem. The tricothecenes are nivalenol, deoxynivalenol, deoxynivalenol-3-glucoside, fusarenon X, 3-acetyl-deoxynivalenol, 15-acetyldeoxynivalenol, diacetoxyscirpenol, and HT-2 and T-2 toxins. Acetic acid and ammonium acetate were used to convert the analytes into their respective acetate adducts and ammonium adducts under negative and positive MS polarity conditions, respectively. The mycotoxins were separated by reversed-phase LC in a 13.5-min run, ionized using electrospray ionization, and detected by tandem mass spectrometry. Analyte-specific mass-to-charge (m/z) ratios were used to perform quantitation under MRM transition and $ MS^{3} $ (linear ion trap) modes. Three experiments were made for each quantitation mode and matrix in batches over 6 days for recovery studies. The matrix effect was investigated at concentration levels of 20, 40, 80, 120, 160, and 200 μg $ kg^{−1} $ (n = 3) in 5 g corn flour and rice flour. Extraction with acetonitrile provided a good overall recovery range of 90–108% (n = 3) at three levels of spiking concentration of 40, 80, and 120 μg $ kg^{−1} $. A quantitation limit of 2–6 μg $ kg^{−1} $ was achieved by applying an MRM transition quantitation strategy. Under $ MS^{3} $ mode, a quantitation limit of 4–10 μg $ kg^{−1} $ was achieved. Relative standard deviations of 2–10% and 2–11% were reported for MRM transition and $ MS^{3} $ quantitation, respectively. The successful utilization of $ MS^{3} $ enabled accurate analyte fragmentation pattern matching and its quantitation, leading to the development of analytical methods in fields that demand both analyte specificity and fragmentation fingerprint-matching capabilities that are unavailable under MRM transition. FigureThe power of QTRAP® technology © Springer-Verlag 2011 |
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Analytical method for the accurate determination of tricothecenes in grains using LC-MS/MS: a comparison between MRM transition and $ MS^{3} $ quantitation |
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https://dx.doi.org/10.1007/s00216-011-5558-2 |
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Tai, Siew Hoon Lee, Lin Min Chan, Sheot Harn |
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10.1007/s00216-011-5558-2 |
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2024-07-04T02:11:07.152Z |
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This paper describes the first accurate LC-MS/MS method developed to analyze tricothecenes in grains by applying multiple reaction monitoring (MRM) transition and $ MS^{3} $ quantitation strategies in tandem. The tricothecenes are nivalenol, deoxynivalenol, deoxynivalenol-3-glucoside, fusarenon X, 3-acetyl-deoxynivalenol, 15-acetyldeoxynivalenol, diacetoxyscirpenol, and HT-2 and T-2 toxins. Acetic acid and ammonium acetate were used to convert the analytes into their respective acetate adducts and ammonium adducts under negative and positive MS polarity conditions, respectively. The mycotoxins were separated by reversed-phase LC in a 13.5-min run, ionized using electrospray ionization, and detected by tandem mass spectrometry. Analyte-specific mass-to-charge (m/z) ratios were used to perform quantitation under MRM transition and $ MS^{3} $ (linear ion trap) modes. Three experiments were made for each quantitation mode and matrix in batches over 6 days for recovery studies. The matrix effect was investigated at concentration levels of 20, 40, 80, 120, 160, and 200 μg $ kg^{−1} $ (n = 3) in 5 g corn flour and rice flour. Extraction with acetonitrile provided a good overall recovery range of 90–108% (n = 3) at three levels of spiking concentration of 40, 80, and 120 μg $ kg^{−1} $. A quantitation limit of 2–6 μg $ kg^{−1} $ was achieved by applying an MRM transition quantitation strategy. Under $ MS^{3} $ mode, a quantitation limit of 4–10 μg $ kg^{−1} $ was achieved. Relative standard deviations of 2–10% and 2–11% were reported for MRM transition and $ MS^{3} $ quantitation, respectively. The successful utilization of $ MS^{3} $ enabled accurate analyte fragmentation pattern matching and its quantitation, leading to the development of analytical methods in fields that demand both analyte specificity and fragmentation fingerprint-matching capabilities that are unavailable under MRM transition. 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