Determination of soyasaponins in Fagioli di Sarconi beans (Phaseolus vulgaris L.) by LC-ESI-FTICR-MS and evaluation of their hypoglycemic activity
Abstract Soyasaponins are oleanene-type triterpenoid saponins, naturally occurring in many edible plants that have attracted a great deal of attention for their role in preventing chronic diseases. The aim of this study was to establish the distribution and the content of soyasaponins in 21 ecotypes...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Bianco, Giuliana [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2017 |
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| Anmerkung: |
© Springer-Verlag GmbH Germany, part of Springer Nature 2017 |
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| Übergeordnetes Werk: |
Enthalten in: Analytical and bioanalytical chemistry - Berlin : Springer, 2002, 410(2017), 5 vom: 21. Dez., Seite 1561-1569 |
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| Übergeordnetes Werk: |
volume:410 ; year:2017 ; number:5 ; day:21 ; month:12 ; pages:1561-1569 |
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| DOI / URN: |
10.1007/s00216-017-0806-8 |
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| Katalog-ID: |
SPR002265362 |
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| 100 | 1 | |a Bianco, Giuliana |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Determination of soyasaponins in Fagioli di Sarconi beans (Phaseolus vulgaris L.) by LC-ESI-FTICR-MS and evaluation of their hypoglycemic activity |
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| 520 | |a Abstract Soyasaponins are oleanene-type triterpenoid saponins, naturally occurring in many edible plants that have attracted a great deal of attention for their role in preventing chronic diseases. The aim of this study was to establish the distribution and the content of soyasaponins in 21 ecotypes of Fagioli di Sarconi beans (Phaseolus vulgaris, Leguminosae). High-performance reversed-phase liquid chromatography (RPLC) with positive electrospray ionization (ESI(+)) and Fourier transform ion cyclotron resonance (FTICR) mass spectrometry (MS) in conjunction with infrared multiphoton dissociation (IRMPD) was applied for the unambiguous identification of soyasaponins Ba (m/z 959.5213, [$ C_{48} %$ H_{79} %$ O_{19} $]+), Bb (m/z 943.5273, [$ C_{48} %$ H_{79} %$ O_{18} $]+), Bd (m/z 957.5122, [$ C_{48} %$ H_{77} %$ O_{19} $]+), and Be (m/z 941.5166, [$ C_{48} %$ H_{77} %$ O_{18} $]+), which are the only commercially available reference standards. In addition, the several diagnostic product ions generated by IRMPD in the ICR-MS cell allowed us the putative identification of soyasaponins Bb' (m/z 797.4680, [$ C_{42} %$ H_{69} %$ O_{14} $]+), αg (m/z 1085.5544, [$ C_{54} %$ H_{85} %$ O_{22} $]+), βg (m/z 1069.5600, [$ C_{54} %$ H_{85} %$ O_{21} $]+), and γg (m/z 923.5009, [$ C_{48} %$ H_{75} %$ O_{17} $]+), establishing thus their membership in the soyasaponin group. Quantitative and semiquantitative analysis of identified soyasaponins were also performed by RPLC-ESI(+) FTICR-MS; the total concentration levels were found ranging from 83.6 ± 9.3 to 767 ± 37 mg/kg. In vitro hypoglycemic outcomes of four soyasaponin standards were evaluated; significant inhibitory activities were obtained with $ IC_{50} $ values ranging from 1.5 ± 0.1 to 2.3 ± 0.2 μg/mL and 12.0 ± 1.1 to 29.4 ± 1.4 μg/mL for α-glucosidase and α-amylase, respectively. This study represents the first detailed investigation on the antidiabetic activity of bioactive constituents found in Fagioli di Sarconi beans. Graphical abstractThe first detailed RPLC-ESI(+) FTICR-MS investigation of the qualitative and semiquantitative profile of soyasaponins, occurring in 21 ecotypes of Fagioli di Sarconi beans (P. vulgaris L.). | ||
| 650 | 4 | |a Soyasaponins |7 (dpeaa)DE-He213 | |
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| 650 | 4 | |a Fourier transform ion cyclotron resonance mass spectrometry |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a FTICR-MS |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a α-Glucosidase |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a α-Amylase |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Pascale, Raffaella |4 aut | |
| 700 | 1 | |a Carbone, Cecilia F. |4 aut | |
| 700 | 1 | |a Acquavia, Maria A. |4 aut | |
| 700 | 1 | |a Cataldi, Tommaso R. I. |4 aut | |
| 700 | 1 | |a Schmitt-Kopplin, Philippe |4 aut | |
| 700 | 1 | |a Buchicchio, Alessandro |4 aut | |
| 700 | 1 | |a Russo, Daniela |4 aut | |
| 700 | 1 | |a Milella, Luigi |4 aut | |
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10.1007/s00216-017-0806-8 doi (DE-627)SPR002265362 (SPR)s00216-017-0806-8-e DE-627 ger DE-627 rakwb eng Bianco, Giuliana verfasserin aut Determination of soyasaponins in Fagioli di Sarconi beans (Phaseolus vulgaris L.) by LC-ESI-FTICR-MS and evaluation of their hypoglycemic activity 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag GmbH Germany, part of Springer Nature 2017 Abstract Soyasaponins are oleanene-type triterpenoid saponins, naturally occurring in many edible plants that have attracted a great deal of attention for their role in preventing chronic diseases. The aim of this study was to establish the distribution and the content of soyasaponins in 21 ecotypes of Fagioli di Sarconi beans (Phaseolus vulgaris, Leguminosae). High-performance reversed-phase liquid chromatography (RPLC) with positive electrospray ionization (ESI(+)) and Fourier transform ion cyclotron resonance (FTICR) mass spectrometry (MS) in conjunction with infrared multiphoton dissociation (IRMPD) was applied for the unambiguous identification of soyasaponins Ba (m/z 959.5213, [$ C_{48} %$ H_{79} %$ O_{19} $]+), Bb (m/z 943.5273, [$ C_{48} %$ H_{79} %$ O_{18} $]+), Bd (m/z 957.5122, [$ C_{48} %$ H_{77} %$ O_{19} $]+), and Be (m/z 941.5166, [$ C_{48} %$ H_{77} %$ O_{18} $]+), which are the only commercially available reference standards. In addition, the several diagnostic product ions generated by IRMPD in the ICR-MS cell allowed us the putative identification of soyasaponins Bb' (m/z 797.4680, [$ C_{42} %$ H_{69} %$ O_{14} $]+), αg (m/z 1085.5544, [$ C_{54} %$ H_{85} %$ O_{22} $]+), βg (m/z 1069.5600, [$ C_{54} %$ H_{85} %$ O_{21} $]+), and γg (m/z 923.5009, [$ C_{48} %$ H_{75} %$ O_{17} $]+), establishing thus their membership in the soyasaponin group. Quantitative and semiquantitative analysis of identified soyasaponins were also performed by RPLC-ESI(+) FTICR-MS; the total concentration levels were found ranging from 83.6 ± 9.3 to 767 ± 37 mg/kg. In vitro hypoglycemic outcomes of four soyasaponin standards were evaluated; significant inhibitory activities were obtained with $ IC_{50} $ values ranging from 1.5 ± 0.1 to 2.3 ± 0.2 μg/mL and 12.0 ± 1.1 to 29.4 ± 1.4 μg/mL for α-glucosidase and α-amylase, respectively. This study represents the first detailed investigation on the antidiabetic activity of bioactive constituents found in Fagioli di Sarconi beans. Graphical abstractThe first detailed RPLC-ESI(+) FTICR-MS investigation of the qualitative and semiquantitative profile of soyasaponins, occurring in 21 ecotypes of Fagioli di Sarconi beans (P. vulgaris L.). Soyasaponins (dpeaa)DE-He213 beans (dpeaa)DE-He213 Fourier transform ion cyclotron resonance mass spectrometry (dpeaa)DE-He213 FTICR-MS (dpeaa)DE-He213 α-Glucosidase (dpeaa)DE-He213 α-Amylase (dpeaa)DE-He213 Pascale, Raffaella aut Carbone, Cecilia F. aut Acquavia, Maria A. aut Cataldi, Tommaso R. I. aut Schmitt-Kopplin, Philippe aut Buchicchio, Alessandro aut Russo, Daniela aut Milella, Luigi aut Enthalten in Analytical and bioanalytical chemistry Berlin : Springer, 2002 410(2017), 5 vom: 21. Dez., Seite 1561-1569 (DE-627)25372337X (DE-600)1459122-4 1618-2650 nnns volume:410 year:2017 number:5 day:21 month:12 pages:1561-1569 https://dx.doi.org/10.1007/s00216-017-0806-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2360 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 410 2017 5 21 12 1561-1569 |
| spelling |
10.1007/s00216-017-0806-8 doi (DE-627)SPR002265362 (SPR)s00216-017-0806-8-e DE-627 ger DE-627 rakwb eng Bianco, Giuliana verfasserin aut Determination of soyasaponins in Fagioli di Sarconi beans (Phaseolus vulgaris L.) by LC-ESI-FTICR-MS and evaluation of their hypoglycemic activity 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag GmbH Germany, part of Springer Nature 2017 Abstract Soyasaponins are oleanene-type triterpenoid saponins, naturally occurring in many edible plants that have attracted a great deal of attention for their role in preventing chronic diseases. The aim of this study was to establish the distribution and the content of soyasaponins in 21 ecotypes of Fagioli di Sarconi beans (Phaseolus vulgaris, Leguminosae). High-performance reversed-phase liquid chromatography (RPLC) with positive electrospray ionization (ESI(+)) and Fourier transform ion cyclotron resonance (FTICR) mass spectrometry (MS) in conjunction with infrared multiphoton dissociation (IRMPD) was applied for the unambiguous identification of soyasaponins Ba (m/z 959.5213, [$ C_{48} %$ H_{79} %$ O_{19} $]+), Bb (m/z 943.5273, [$ C_{48} %$ H_{79} %$ O_{18} $]+), Bd (m/z 957.5122, [$ C_{48} %$ H_{77} %$ O_{19} $]+), and Be (m/z 941.5166, [$ C_{48} %$ H_{77} %$ O_{18} $]+), which are the only commercially available reference standards. In addition, the several diagnostic product ions generated by IRMPD in the ICR-MS cell allowed us the putative identification of soyasaponins Bb' (m/z 797.4680, [$ C_{42} %$ H_{69} %$ O_{14} $]+), αg (m/z 1085.5544, [$ C_{54} %$ H_{85} %$ O_{22} $]+), βg (m/z 1069.5600, [$ C_{54} %$ H_{85} %$ O_{21} $]+), and γg (m/z 923.5009, [$ C_{48} %$ H_{75} %$ O_{17} $]+), establishing thus their membership in the soyasaponin group. Quantitative and semiquantitative analysis of identified soyasaponins were also performed by RPLC-ESI(+) FTICR-MS; the total concentration levels were found ranging from 83.6 ± 9.3 to 767 ± 37 mg/kg. In vitro hypoglycemic outcomes of four soyasaponin standards were evaluated; significant inhibitory activities were obtained with $ IC_{50} $ values ranging from 1.5 ± 0.1 to 2.3 ± 0.2 μg/mL and 12.0 ± 1.1 to 29.4 ± 1.4 μg/mL for α-glucosidase and α-amylase, respectively. This study represents the first detailed investigation on the antidiabetic activity of bioactive constituents found in Fagioli di Sarconi beans. Graphical abstractThe first detailed RPLC-ESI(+) FTICR-MS investigation of the qualitative and semiquantitative profile of soyasaponins, occurring in 21 ecotypes of Fagioli di Sarconi beans (P. vulgaris L.). Soyasaponins (dpeaa)DE-He213 beans (dpeaa)DE-He213 Fourier transform ion cyclotron resonance mass spectrometry (dpeaa)DE-He213 FTICR-MS (dpeaa)DE-He213 α-Glucosidase (dpeaa)DE-He213 α-Amylase (dpeaa)DE-He213 Pascale, Raffaella aut Carbone, Cecilia F. aut Acquavia, Maria A. aut Cataldi, Tommaso R. I. aut Schmitt-Kopplin, Philippe aut Buchicchio, Alessandro aut Russo, Daniela aut Milella, Luigi aut Enthalten in Analytical and bioanalytical chemistry Berlin : Springer, 2002 410(2017), 5 vom: 21. Dez., Seite 1561-1569 (DE-627)25372337X (DE-600)1459122-4 1618-2650 nnns volume:410 year:2017 number:5 day:21 month:12 pages:1561-1569 https://dx.doi.org/10.1007/s00216-017-0806-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2360 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 410 2017 5 21 12 1561-1569 |
| allfields_unstemmed |
10.1007/s00216-017-0806-8 doi (DE-627)SPR002265362 (SPR)s00216-017-0806-8-e DE-627 ger DE-627 rakwb eng Bianco, Giuliana verfasserin aut Determination of soyasaponins in Fagioli di Sarconi beans (Phaseolus vulgaris L.) by LC-ESI-FTICR-MS and evaluation of their hypoglycemic activity 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag GmbH Germany, part of Springer Nature 2017 Abstract Soyasaponins are oleanene-type triterpenoid saponins, naturally occurring in many edible plants that have attracted a great deal of attention for their role in preventing chronic diseases. The aim of this study was to establish the distribution and the content of soyasaponins in 21 ecotypes of Fagioli di Sarconi beans (Phaseolus vulgaris, Leguminosae). High-performance reversed-phase liquid chromatography (RPLC) with positive electrospray ionization (ESI(+)) and Fourier transform ion cyclotron resonance (FTICR) mass spectrometry (MS) in conjunction with infrared multiphoton dissociation (IRMPD) was applied for the unambiguous identification of soyasaponins Ba (m/z 959.5213, [$ C_{48} %$ H_{79} %$ O_{19} $]+), Bb (m/z 943.5273, [$ C_{48} %$ H_{79} %$ O_{18} $]+), Bd (m/z 957.5122, [$ C_{48} %$ H_{77} %$ O_{19} $]+), and Be (m/z 941.5166, [$ C_{48} %$ H_{77} %$ O_{18} $]+), which are the only commercially available reference standards. In addition, the several diagnostic product ions generated by IRMPD in the ICR-MS cell allowed us the putative identification of soyasaponins Bb' (m/z 797.4680, [$ C_{42} %$ H_{69} %$ O_{14} $]+), αg (m/z 1085.5544, [$ C_{54} %$ H_{85} %$ O_{22} $]+), βg (m/z 1069.5600, [$ C_{54} %$ H_{85} %$ O_{21} $]+), and γg (m/z 923.5009, [$ C_{48} %$ H_{75} %$ O_{17} $]+), establishing thus their membership in the soyasaponin group. Quantitative and semiquantitative analysis of identified soyasaponins were also performed by RPLC-ESI(+) FTICR-MS; the total concentration levels were found ranging from 83.6 ± 9.3 to 767 ± 37 mg/kg. In vitro hypoglycemic outcomes of four soyasaponin standards were evaluated; significant inhibitory activities were obtained with $ IC_{50} $ values ranging from 1.5 ± 0.1 to 2.3 ± 0.2 μg/mL and 12.0 ± 1.1 to 29.4 ± 1.4 μg/mL for α-glucosidase and α-amylase, respectively. This study represents the first detailed investigation on the antidiabetic activity of bioactive constituents found in Fagioli di Sarconi beans. Graphical abstractThe first detailed RPLC-ESI(+) FTICR-MS investigation of the qualitative and semiquantitative profile of soyasaponins, occurring in 21 ecotypes of Fagioli di Sarconi beans (P. vulgaris L.). Soyasaponins (dpeaa)DE-He213 beans (dpeaa)DE-He213 Fourier transform ion cyclotron resonance mass spectrometry (dpeaa)DE-He213 FTICR-MS (dpeaa)DE-He213 α-Glucosidase (dpeaa)DE-He213 α-Amylase (dpeaa)DE-He213 Pascale, Raffaella aut Carbone, Cecilia F. aut Acquavia, Maria A. aut Cataldi, Tommaso R. I. aut Schmitt-Kopplin, Philippe aut Buchicchio, Alessandro aut Russo, Daniela aut Milella, Luigi aut Enthalten in Analytical and bioanalytical chemistry Berlin : Springer, 2002 410(2017), 5 vom: 21. Dez., Seite 1561-1569 (DE-627)25372337X (DE-600)1459122-4 1618-2650 nnns volume:410 year:2017 number:5 day:21 month:12 pages:1561-1569 https://dx.doi.org/10.1007/s00216-017-0806-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2360 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 410 2017 5 21 12 1561-1569 |
| allfieldsGer |
10.1007/s00216-017-0806-8 doi (DE-627)SPR002265362 (SPR)s00216-017-0806-8-e DE-627 ger DE-627 rakwb eng Bianco, Giuliana verfasserin aut Determination of soyasaponins in Fagioli di Sarconi beans (Phaseolus vulgaris L.) by LC-ESI-FTICR-MS and evaluation of their hypoglycemic activity 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag GmbH Germany, part of Springer Nature 2017 Abstract Soyasaponins are oleanene-type triterpenoid saponins, naturally occurring in many edible plants that have attracted a great deal of attention for their role in preventing chronic diseases. The aim of this study was to establish the distribution and the content of soyasaponins in 21 ecotypes of Fagioli di Sarconi beans (Phaseolus vulgaris, Leguminosae). High-performance reversed-phase liquid chromatography (RPLC) with positive electrospray ionization (ESI(+)) and Fourier transform ion cyclotron resonance (FTICR) mass spectrometry (MS) in conjunction with infrared multiphoton dissociation (IRMPD) was applied for the unambiguous identification of soyasaponins Ba (m/z 959.5213, [$ C_{48} %$ H_{79} %$ O_{19} $]+), Bb (m/z 943.5273, [$ C_{48} %$ H_{79} %$ O_{18} $]+), Bd (m/z 957.5122, [$ C_{48} %$ H_{77} %$ O_{19} $]+), and Be (m/z 941.5166, [$ C_{48} %$ H_{77} %$ O_{18} $]+), which are the only commercially available reference standards. In addition, the several diagnostic product ions generated by IRMPD in the ICR-MS cell allowed us the putative identification of soyasaponins Bb' (m/z 797.4680, [$ C_{42} %$ H_{69} %$ O_{14} $]+), αg (m/z 1085.5544, [$ C_{54} %$ H_{85} %$ O_{22} $]+), βg (m/z 1069.5600, [$ C_{54} %$ H_{85} %$ O_{21} $]+), and γg (m/z 923.5009, [$ C_{48} %$ H_{75} %$ O_{17} $]+), establishing thus their membership in the soyasaponin group. Quantitative and semiquantitative analysis of identified soyasaponins were also performed by RPLC-ESI(+) FTICR-MS; the total concentration levels were found ranging from 83.6 ± 9.3 to 767 ± 37 mg/kg. In vitro hypoglycemic outcomes of four soyasaponin standards were evaluated; significant inhibitory activities were obtained with $ IC_{50} $ values ranging from 1.5 ± 0.1 to 2.3 ± 0.2 μg/mL and 12.0 ± 1.1 to 29.4 ± 1.4 μg/mL for α-glucosidase and α-amylase, respectively. This study represents the first detailed investigation on the antidiabetic activity of bioactive constituents found in Fagioli di Sarconi beans. Graphical abstractThe first detailed RPLC-ESI(+) FTICR-MS investigation of the qualitative and semiquantitative profile of soyasaponins, occurring in 21 ecotypes of Fagioli di Sarconi beans (P. vulgaris L.). Soyasaponins (dpeaa)DE-He213 beans (dpeaa)DE-He213 Fourier transform ion cyclotron resonance mass spectrometry (dpeaa)DE-He213 FTICR-MS (dpeaa)DE-He213 α-Glucosidase (dpeaa)DE-He213 α-Amylase (dpeaa)DE-He213 Pascale, Raffaella aut Carbone, Cecilia F. aut Acquavia, Maria A. aut Cataldi, Tommaso R. I. aut Schmitt-Kopplin, Philippe aut Buchicchio, Alessandro aut Russo, Daniela aut Milella, Luigi aut Enthalten in Analytical and bioanalytical chemistry Berlin : Springer, 2002 410(2017), 5 vom: 21. Dez., Seite 1561-1569 (DE-627)25372337X (DE-600)1459122-4 1618-2650 nnns volume:410 year:2017 number:5 day:21 month:12 pages:1561-1569 https://dx.doi.org/10.1007/s00216-017-0806-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2360 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 410 2017 5 21 12 1561-1569 |
| allfieldsSound |
10.1007/s00216-017-0806-8 doi (DE-627)SPR002265362 (SPR)s00216-017-0806-8-e DE-627 ger DE-627 rakwb eng Bianco, Giuliana verfasserin aut Determination of soyasaponins in Fagioli di Sarconi beans (Phaseolus vulgaris L.) by LC-ESI-FTICR-MS and evaluation of their hypoglycemic activity 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag GmbH Germany, part of Springer Nature 2017 Abstract Soyasaponins are oleanene-type triterpenoid saponins, naturally occurring in many edible plants that have attracted a great deal of attention for their role in preventing chronic diseases. The aim of this study was to establish the distribution and the content of soyasaponins in 21 ecotypes of Fagioli di Sarconi beans (Phaseolus vulgaris, Leguminosae). High-performance reversed-phase liquid chromatography (RPLC) with positive electrospray ionization (ESI(+)) and Fourier transform ion cyclotron resonance (FTICR) mass spectrometry (MS) in conjunction with infrared multiphoton dissociation (IRMPD) was applied for the unambiguous identification of soyasaponins Ba (m/z 959.5213, [$ C_{48} %$ H_{79} %$ O_{19} $]+), Bb (m/z 943.5273, [$ C_{48} %$ H_{79} %$ O_{18} $]+), Bd (m/z 957.5122, [$ C_{48} %$ H_{77} %$ O_{19} $]+), and Be (m/z 941.5166, [$ C_{48} %$ H_{77} %$ O_{18} $]+), which are the only commercially available reference standards. In addition, the several diagnostic product ions generated by IRMPD in the ICR-MS cell allowed us the putative identification of soyasaponins Bb' (m/z 797.4680, [$ C_{42} %$ H_{69} %$ O_{14} $]+), αg (m/z 1085.5544, [$ C_{54} %$ H_{85} %$ O_{22} $]+), βg (m/z 1069.5600, [$ C_{54} %$ H_{85} %$ O_{21} $]+), and γg (m/z 923.5009, [$ C_{48} %$ H_{75} %$ O_{17} $]+), establishing thus their membership in the soyasaponin group. Quantitative and semiquantitative analysis of identified soyasaponins were also performed by RPLC-ESI(+) FTICR-MS; the total concentration levels were found ranging from 83.6 ± 9.3 to 767 ± 37 mg/kg. In vitro hypoglycemic outcomes of four soyasaponin standards were evaluated; significant inhibitory activities were obtained with $ IC_{50} $ values ranging from 1.5 ± 0.1 to 2.3 ± 0.2 μg/mL and 12.0 ± 1.1 to 29.4 ± 1.4 μg/mL for α-glucosidase and α-amylase, respectively. This study represents the first detailed investigation on the antidiabetic activity of bioactive constituents found in Fagioli di Sarconi beans. Graphical abstractThe first detailed RPLC-ESI(+) FTICR-MS investigation of the qualitative and semiquantitative profile of soyasaponins, occurring in 21 ecotypes of Fagioli di Sarconi beans (P. vulgaris L.). Soyasaponins (dpeaa)DE-He213 beans (dpeaa)DE-He213 Fourier transform ion cyclotron resonance mass spectrometry (dpeaa)DE-He213 FTICR-MS (dpeaa)DE-He213 α-Glucosidase (dpeaa)DE-He213 α-Amylase (dpeaa)DE-He213 Pascale, Raffaella aut Carbone, Cecilia F. aut Acquavia, Maria A. aut Cataldi, Tommaso R. I. aut Schmitt-Kopplin, Philippe aut Buchicchio, Alessandro aut Russo, Daniela aut Milella, Luigi aut Enthalten in Analytical and bioanalytical chemistry Berlin : Springer, 2002 410(2017), 5 vom: 21. Dez., Seite 1561-1569 (DE-627)25372337X (DE-600)1459122-4 1618-2650 nnns volume:410 year:2017 number:5 day:21 month:12 pages:1561-1569 https://dx.doi.org/10.1007/s00216-017-0806-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2360 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 410 2017 5 21 12 1561-1569 |
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Enthalten in Analytical and bioanalytical chemistry 410(2017), 5 vom: 21. Dez., Seite 1561-1569 volume:410 year:2017 number:5 day:21 month:12 pages:1561-1569 |
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Enthalten in Analytical and bioanalytical chemistry 410(2017), 5 vom: 21. Dez., Seite 1561-1569 volume:410 year:2017 number:5 day:21 month:12 pages:1561-1569 |
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Soyasaponins beans Fourier transform ion cyclotron resonance mass spectrometry FTICR-MS α-Glucosidase α-Amylase |
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Analytical and bioanalytical chemistry |
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Bianco, Giuliana @@aut@@ Pascale, Raffaella @@aut@@ Carbone, Cecilia F. @@aut@@ Acquavia, Maria A. @@aut@@ Cataldi, Tommaso R. I. @@aut@@ Schmitt-Kopplin, Philippe @@aut@@ Buchicchio, Alessandro @@aut@@ Russo, Daniela @@aut@@ Milella, Luigi @@aut@@ |
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2017-12-21T00:00:00Z |
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The aim of this study was to establish the distribution and the content of soyasaponins in 21 ecotypes of Fagioli di Sarconi beans (Phaseolus vulgaris, Leguminosae). High-performance reversed-phase liquid chromatography (RPLC) with positive electrospray ionization (ESI(+)) and Fourier transform ion cyclotron resonance (FTICR) mass spectrometry (MS) in conjunction with infrared multiphoton dissociation (IRMPD) was applied for the unambiguous identification of soyasaponins Ba (m/z 959.5213, [$ C_{48} %$ H_{79} %$ O_{19} $]+), Bb (m/z 943.5273, [$ C_{48} %$ H_{79} %$ O_{18} $]+), Bd (m/z 957.5122, [$ C_{48} %$ H_{77} %$ O_{19} $]+), and Be (m/z 941.5166, [$ C_{48} %$ H_{77} %$ O_{18} $]+), which are the only commercially available reference standards. In addition, the several diagnostic product ions generated by IRMPD in the ICR-MS cell allowed us the putative identification of soyasaponins Bb' (m/z 797.4680, [$ C_{42} %$ H_{69} %$ O_{14} $]+), αg (m/z 1085.5544, [$ C_{54} %$ H_{85} %$ O_{22} $]+), βg (m/z 1069.5600, [$ C_{54} %$ H_{85} %$ O_{21} $]+), and γg (m/z 923.5009, [$ C_{48} %$ H_{75} %$ O_{17} $]+), establishing thus their membership in the soyasaponin group. Quantitative and semiquantitative analysis of identified soyasaponins were also performed by RPLC-ESI(+) FTICR-MS; the total concentration levels were found ranging from 83.6 ± 9.3 to 767 ± 37 mg/kg. In vitro hypoglycemic outcomes of four soyasaponin standards were evaluated; significant inhibitory activities were obtained with $ IC_{50} $ values ranging from 1.5 ± 0.1 to 2.3 ± 0.2 μg/mL and 12.0 ± 1.1 to 29.4 ± 1.4 μg/mL for α-glucosidase and α-amylase, respectively. This study represents the first detailed investigation on the antidiabetic activity of bioactive constituents found in Fagioli di Sarconi beans. 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Bianco, Giuliana |
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Bianco, Giuliana misc Soyasaponins misc beans misc Fourier transform ion cyclotron resonance mass spectrometry misc FTICR-MS misc α-Glucosidase misc α-Amylase Determination of soyasaponins in Fagioli di Sarconi beans (Phaseolus vulgaris L.) by LC-ESI-FTICR-MS and evaluation of their hypoglycemic activity |
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Determination of soyasaponins in Fagioli di Sarconi beans (Phaseolus vulgaris L.) by LC-ESI-FTICR-MS and evaluation of their hypoglycemic activity Soyasaponins (dpeaa)DE-He213 beans (dpeaa)DE-He213 Fourier transform ion cyclotron resonance mass spectrometry (dpeaa)DE-He213 FTICR-MS (dpeaa)DE-He213 α-Glucosidase (dpeaa)DE-He213 α-Amylase (dpeaa)DE-He213 |
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Determination of soyasaponins in Fagioli di Sarconi beans (Phaseolus vulgaris L.) by LC-ESI-FTICR-MS and evaluation of their hypoglycemic activity |
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Determination of soyasaponins in Fagioli di Sarconi beans (Phaseolus vulgaris L.) by LC-ESI-FTICR-MS and evaluation of their hypoglycemic activity |
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Bianco, Giuliana Pascale, Raffaella Carbone, Cecilia F. Acquavia, Maria A. Cataldi, Tommaso R. I. Schmitt-Kopplin, Philippe Buchicchio, Alessandro Russo, Daniela Milella, Luigi |
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determination of soyasaponins in fagioli di sarconi beans (phaseolus vulgaris l.) by lc-esi-fticr-ms and evaluation of their hypoglycemic activity |
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Determination of soyasaponins in Fagioli di Sarconi beans (Phaseolus vulgaris L.) by LC-ESI-FTICR-MS and evaluation of their hypoglycemic activity |
| abstract |
Abstract Soyasaponins are oleanene-type triterpenoid saponins, naturally occurring in many edible plants that have attracted a great deal of attention for their role in preventing chronic diseases. The aim of this study was to establish the distribution and the content of soyasaponins in 21 ecotypes of Fagioli di Sarconi beans (Phaseolus vulgaris, Leguminosae). High-performance reversed-phase liquid chromatography (RPLC) with positive electrospray ionization (ESI(+)) and Fourier transform ion cyclotron resonance (FTICR) mass spectrometry (MS) in conjunction with infrared multiphoton dissociation (IRMPD) was applied for the unambiguous identification of soyasaponins Ba (m/z 959.5213, [$ C_{48} %$ H_{79} %$ O_{19} $]+), Bb (m/z 943.5273, [$ C_{48} %$ H_{79} %$ O_{18} $]+), Bd (m/z 957.5122, [$ C_{48} %$ H_{77} %$ O_{19} $]+), and Be (m/z 941.5166, [$ C_{48} %$ H_{77} %$ O_{18} $]+), which are the only commercially available reference standards. In addition, the several diagnostic product ions generated by IRMPD in the ICR-MS cell allowed us the putative identification of soyasaponins Bb' (m/z 797.4680, [$ C_{42} %$ H_{69} %$ O_{14} $]+), αg (m/z 1085.5544, [$ C_{54} %$ H_{85} %$ O_{22} $]+), βg (m/z 1069.5600, [$ C_{54} %$ H_{85} %$ O_{21} $]+), and γg (m/z 923.5009, [$ C_{48} %$ H_{75} %$ O_{17} $]+), establishing thus their membership in the soyasaponin group. Quantitative and semiquantitative analysis of identified soyasaponins were also performed by RPLC-ESI(+) FTICR-MS; the total concentration levels were found ranging from 83.6 ± 9.3 to 767 ± 37 mg/kg. In vitro hypoglycemic outcomes of four soyasaponin standards were evaluated; significant inhibitory activities were obtained with $ IC_{50} $ values ranging from 1.5 ± 0.1 to 2.3 ± 0.2 μg/mL and 12.0 ± 1.1 to 29.4 ± 1.4 μg/mL for α-glucosidase and α-amylase, respectively. This study represents the first detailed investigation on the antidiabetic activity of bioactive constituents found in Fagioli di Sarconi beans. Graphical abstractThe first detailed RPLC-ESI(+) FTICR-MS investigation of the qualitative and semiquantitative profile of soyasaponins, occurring in 21 ecotypes of Fagioli di Sarconi beans (P. vulgaris L.). © Springer-Verlag GmbH Germany, part of Springer Nature 2017 |
| abstractGer |
Abstract Soyasaponins are oleanene-type triterpenoid saponins, naturally occurring in many edible plants that have attracted a great deal of attention for their role in preventing chronic diseases. The aim of this study was to establish the distribution and the content of soyasaponins in 21 ecotypes of Fagioli di Sarconi beans (Phaseolus vulgaris, Leguminosae). High-performance reversed-phase liquid chromatography (RPLC) with positive electrospray ionization (ESI(+)) and Fourier transform ion cyclotron resonance (FTICR) mass spectrometry (MS) in conjunction with infrared multiphoton dissociation (IRMPD) was applied for the unambiguous identification of soyasaponins Ba (m/z 959.5213, [$ C_{48} %$ H_{79} %$ O_{19} $]+), Bb (m/z 943.5273, [$ C_{48} %$ H_{79} %$ O_{18} $]+), Bd (m/z 957.5122, [$ C_{48} %$ H_{77} %$ O_{19} $]+), and Be (m/z 941.5166, [$ C_{48} %$ H_{77} %$ O_{18} $]+), which are the only commercially available reference standards. In addition, the several diagnostic product ions generated by IRMPD in the ICR-MS cell allowed us the putative identification of soyasaponins Bb' (m/z 797.4680, [$ C_{42} %$ H_{69} %$ O_{14} $]+), αg (m/z 1085.5544, [$ C_{54} %$ H_{85} %$ O_{22} $]+), βg (m/z 1069.5600, [$ C_{54} %$ H_{85} %$ O_{21} $]+), and γg (m/z 923.5009, [$ C_{48} %$ H_{75} %$ O_{17} $]+), establishing thus their membership in the soyasaponin group. Quantitative and semiquantitative analysis of identified soyasaponins were also performed by RPLC-ESI(+) FTICR-MS; the total concentration levels were found ranging from 83.6 ± 9.3 to 767 ± 37 mg/kg. In vitro hypoglycemic outcomes of four soyasaponin standards were evaluated; significant inhibitory activities were obtained with $ IC_{50} $ values ranging from 1.5 ± 0.1 to 2.3 ± 0.2 μg/mL and 12.0 ± 1.1 to 29.4 ± 1.4 μg/mL for α-glucosidase and α-amylase, respectively. This study represents the first detailed investigation on the antidiabetic activity of bioactive constituents found in Fagioli di Sarconi beans. Graphical abstractThe first detailed RPLC-ESI(+) FTICR-MS investigation of the qualitative and semiquantitative profile of soyasaponins, occurring in 21 ecotypes of Fagioli di Sarconi beans (P. vulgaris L.). © Springer-Verlag GmbH Germany, part of Springer Nature 2017 |
| abstract_unstemmed |
Abstract Soyasaponins are oleanene-type triterpenoid saponins, naturally occurring in many edible plants that have attracted a great deal of attention for their role in preventing chronic diseases. The aim of this study was to establish the distribution and the content of soyasaponins in 21 ecotypes of Fagioli di Sarconi beans (Phaseolus vulgaris, Leguminosae). High-performance reversed-phase liquid chromatography (RPLC) with positive electrospray ionization (ESI(+)) and Fourier transform ion cyclotron resonance (FTICR) mass spectrometry (MS) in conjunction with infrared multiphoton dissociation (IRMPD) was applied for the unambiguous identification of soyasaponins Ba (m/z 959.5213, [$ C_{48} %$ H_{79} %$ O_{19} $]+), Bb (m/z 943.5273, [$ C_{48} %$ H_{79} %$ O_{18} $]+), Bd (m/z 957.5122, [$ C_{48} %$ H_{77} %$ O_{19} $]+), and Be (m/z 941.5166, [$ C_{48} %$ H_{77} %$ O_{18} $]+), which are the only commercially available reference standards. In addition, the several diagnostic product ions generated by IRMPD in the ICR-MS cell allowed us the putative identification of soyasaponins Bb' (m/z 797.4680, [$ C_{42} %$ H_{69} %$ O_{14} $]+), αg (m/z 1085.5544, [$ C_{54} %$ H_{85} %$ O_{22} $]+), βg (m/z 1069.5600, [$ C_{54} %$ H_{85} %$ O_{21} $]+), and γg (m/z 923.5009, [$ C_{48} %$ H_{75} %$ O_{17} $]+), establishing thus their membership in the soyasaponin group. Quantitative and semiquantitative analysis of identified soyasaponins were also performed by RPLC-ESI(+) FTICR-MS; the total concentration levels were found ranging from 83.6 ± 9.3 to 767 ± 37 mg/kg. In vitro hypoglycemic outcomes of four soyasaponin standards were evaluated; significant inhibitory activities were obtained with $ IC_{50} $ values ranging from 1.5 ± 0.1 to 2.3 ± 0.2 μg/mL and 12.0 ± 1.1 to 29.4 ± 1.4 μg/mL for α-glucosidase and α-amylase, respectively. This study represents the first detailed investigation on the antidiabetic activity of bioactive constituents found in Fagioli di Sarconi beans. Graphical abstractThe first detailed RPLC-ESI(+) FTICR-MS investigation of the qualitative and semiquantitative profile of soyasaponins, occurring in 21 ecotypes of Fagioli di Sarconi beans (P. vulgaris L.). © Springer-Verlag GmbH Germany, part of Springer Nature 2017 |
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Determination of soyasaponins in Fagioli di Sarconi beans (Phaseolus vulgaris L.) by LC-ESI-FTICR-MS and evaluation of their hypoglycemic activity |
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The aim of this study was to establish the distribution and the content of soyasaponins in 21 ecotypes of Fagioli di Sarconi beans (Phaseolus vulgaris, Leguminosae). High-performance reversed-phase liquid chromatography (RPLC) with positive electrospray ionization (ESI(+)) and Fourier transform ion cyclotron resonance (FTICR) mass spectrometry (MS) in conjunction with infrared multiphoton dissociation (IRMPD) was applied for the unambiguous identification of soyasaponins Ba (m/z 959.5213, [$ C_{48} %$ H_{79} %$ O_{19} $]+), Bb (m/z 943.5273, [$ C_{48} %$ H_{79} %$ O_{18} $]+), Bd (m/z 957.5122, [$ C_{48} %$ H_{77} %$ O_{19} $]+), and Be (m/z 941.5166, [$ C_{48} %$ H_{77} %$ O_{18} $]+), which are the only commercially available reference standards. In addition, the several diagnostic product ions generated by IRMPD in the ICR-MS cell allowed us the putative identification of soyasaponins Bb' (m/z 797.4680, [$ C_{42} %$ H_{69} %$ O_{14} $]+), αg (m/z 1085.5544, [$ C_{54} %$ H_{85} %$ O_{22} $]+), βg (m/z 1069.5600, [$ C_{54} %$ H_{85} %$ O_{21} $]+), and γg (m/z 923.5009, [$ C_{48} %$ H_{75} %$ O_{17} $]+), establishing thus their membership in the soyasaponin group. Quantitative and semiquantitative analysis of identified soyasaponins were also performed by RPLC-ESI(+) FTICR-MS; the total concentration levels were found ranging from 83.6 ± 9.3 to 767 ± 37 mg/kg. In vitro hypoglycemic outcomes of four soyasaponin standards were evaluated; significant inhibitory activities were obtained with $ IC_{50} $ values ranging from 1.5 ± 0.1 to 2.3 ± 0.2 μg/mL and 12.0 ± 1.1 to 29.4 ± 1.4 μg/mL for α-glucosidase and α-amylase, respectively. This study represents the first detailed investigation on the antidiabetic activity of bioactive constituents found in Fagioli di Sarconi beans. Graphical abstractThe first detailed RPLC-ESI(+) FTICR-MS investigation of the qualitative and semiquantitative profile of soyasaponins, occurring in 21 ecotypes of Fagioli di Sarconi beans (P. vulgaris L.).</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Soyasaponins</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">beans</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Fourier transform ion cyclotron resonance mass spectrometry</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">FTICR-MS</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">α-Glucosidase</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">α-Amylase</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Pascale, Raffaella</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Carbone, Cecilia F.</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Acquavia, Maria A.</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Cataldi, Tommaso R. 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