Detection of genetically modified soybean and its product tou-kan by polymerase chain reaction with dual pairs of DNA primers
Abstract Since genetically modified (GM) crops and foods began to appear in market, the detection of these GM foods has become an important issue. Efficient and reliable methods are urgently needed to analyze the GM content of a large quantity of foods. However, most foods are processed through heat...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Liu, Yi-Ching [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2005 |
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| Schlagwörter: |
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| Anmerkung: |
© Springer-Verlag 2005 |
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| Übergeordnetes Werk: |
Enthalten in: European food research and technology - Berlin : Springer, 1999, 221(2005), 6 vom: 04. Aug., Seite 725-730 |
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| Übergeordnetes Werk: |
volume:221 ; year:2005 ; number:6 ; day:04 ; month:08 ; pages:725-730 |
| Links: |
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| DOI / URN: |
10.1007/s00217-005-0066-2 |
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| Katalog-ID: |
SPR002288303 |
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| 100 | 1 | |a Liu, Yi-Ching |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Detection of genetically modified soybean and its product tou-kan by polymerase chain reaction with dual pairs of DNA primers |
| 264 | 1 | |c 2005 | |
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| 520 | |a Abstract Since genetically modified (GM) crops and foods began to appear in market, the detection of these GM foods has become an important issue. Efficient and reliable methods are urgently needed to analyze the GM content of a large quantity of foods. However, most foods are processed through heating or cooking, and their proteins are denatured. Therefore, DNA rather than protein is the major target for detecting GM foods. In this report, polymerase chain reaction (PCR) was performed with dual sets of DNA primers, which co-amplified the soybean-specific lectin gene and the transgenic petunia transit peptide sequence (CTP) in the 5′ end of the 5-enol-pyruvyl-shikimate-3-phosphate synthase (EPSPS) gene within the same reaction. PCR with these two sets of primers reacted specifically with lectin gene and CTP, and can detect soybean with GM content less than 1%. PCR detecting both lectin gene and CTP was also applied to a soybean-derived product tou-kan, a traditional oriental food. Results indicated that although DNA was partially degraded in tou-kan, this PCR method was still able to detect the presence of EPSPS gene. However, when the DNA within tou-kan was destroyed badly, neither lectin nor EPSPS genes were detected by the PCR suggested here. Finally, an examination procedure for the Roundup Ready soybean was suggested according to the results of PCR with dual pairs of DNA primers. The proposed PCR method has proved to be a reliable and efficient method for detecting the GM content of the processed foods from Roundup Ready soybean. | ||
| 650 | 4 | |a Genetically modified soybean |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Tou-kan |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Polymerase chain reaction |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Lin, Shin-Huang |4 aut | |
| 700 | 1 | |a Lai, Hsi-Mei |4 aut | |
| 700 | 1 | |a Jeng, Shih-Tong |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t European food research and technology |d Berlin : Springer, 1999 |g 221(2005), 6 vom: 04. Aug., Seite 725-730 |w (DE-627)27012859X |w (DE-600)1476605-X |x 1438-2385 |7 nnns |
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10.1007/s00217-005-0066-2 doi (DE-627)SPR002288303 (SPR)s00217-005-0066-2-e DE-627 ger DE-627 rakwb eng Liu, Yi-Ching verfasserin aut Detection of genetically modified soybean and its product tou-kan by polymerase chain reaction with dual pairs of DNA primers 2005 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag 2005 Abstract Since genetically modified (GM) crops and foods began to appear in market, the detection of these GM foods has become an important issue. Efficient and reliable methods are urgently needed to analyze the GM content of a large quantity of foods. However, most foods are processed through heating or cooking, and their proteins are denatured. Therefore, DNA rather than protein is the major target for detecting GM foods. In this report, polymerase chain reaction (PCR) was performed with dual sets of DNA primers, which co-amplified the soybean-specific lectin gene and the transgenic petunia transit peptide sequence (CTP) in the 5′ end of the 5-enol-pyruvyl-shikimate-3-phosphate synthase (EPSPS) gene within the same reaction. PCR with these two sets of primers reacted specifically with lectin gene and CTP, and can detect soybean with GM content less than 1%. PCR detecting both lectin gene and CTP was also applied to a soybean-derived product tou-kan, a traditional oriental food. Results indicated that although DNA was partially degraded in tou-kan, this PCR method was still able to detect the presence of EPSPS gene. However, when the DNA within tou-kan was destroyed badly, neither lectin nor EPSPS genes were detected by the PCR suggested here. Finally, an examination procedure for the Roundup Ready soybean was suggested according to the results of PCR with dual pairs of DNA primers. The proposed PCR method has proved to be a reliable and efficient method for detecting the GM content of the processed foods from Roundup Ready soybean. Genetically modified soybean (dpeaa)DE-He213 Tou-kan (dpeaa)DE-He213 Polymerase chain reaction (dpeaa)DE-He213 Lin, Shin-Huang aut Lai, Hsi-Mei aut Jeng, Shih-Tong aut Enthalten in European food research and technology Berlin : Springer, 1999 221(2005), 6 vom: 04. Aug., Seite 725-730 (DE-627)27012859X (DE-600)1476605-X 1438-2385 nnns volume:221 year:2005 number:6 day:04 month:08 pages:725-730 https://dx.doi.org/10.1007/s00217-005-0066-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 221 2005 6 04 08 725-730 |
| spelling |
10.1007/s00217-005-0066-2 doi (DE-627)SPR002288303 (SPR)s00217-005-0066-2-e DE-627 ger DE-627 rakwb eng Liu, Yi-Ching verfasserin aut Detection of genetically modified soybean and its product tou-kan by polymerase chain reaction with dual pairs of DNA primers 2005 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag 2005 Abstract Since genetically modified (GM) crops and foods began to appear in market, the detection of these GM foods has become an important issue. Efficient and reliable methods are urgently needed to analyze the GM content of a large quantity of foods. However, most foods are processed through heating or cooking, and their proteins are denatured. Therefore, DNA rather than protein is the major target for detecting GM foods. In this report, polymerase chain reaction (PCR) was performed with dual sets of DNA primers, which co-amplified the soybean-specific lectin gene and the transgenic petunia transit peptide sequence (CTP) in the 5′ end of the 5-enol-pyruvyl-shikimate-3-phosphate synthase (EPSPS) gene within the same reaction. PCR with these two sets of primers reacted specifically with lectin gene and CTP, and can detect soybean with GM content less than 1%. PCR detecting both lectin gene and CTP was also applied to a soybean-derived product tou-kan, a traditional oriental food. Results indicated that although DNA was partially degraded in tou-kan, this PCR method was still able to detect the presence of EPSPS gene. However, when the DNA within tou-kan was destroyed badly, neither lectin nor EPSPS genes were detected by the PCR suggested here. Finally, an examination procedure for the Roundup Ready soybean was suggested according to the results of PCR with dual pairs of DNA primers. The proposed PCR method has proved to be a reliable and efficient method for detecting the GM content of the processed foods from Roundup Ready soybean. Genetically modified soybean (dpeaa)DE-He213 Tou-kan (dpeaa)DE-He213 Polymerase chain reaction (dpeaa)DE-He213 Lin, Shin-Huang aut Lai, Hsi-Mei aut Jeng, Shih-Tong aut Enthalten in European food research and technology Berlin : Springer, 1999 221(2005), 6 vom: 04. Aug., Seite 725-730 (DE-627)27012859X (DE-600)1476605-X 1438-2385 nnns volume:221 year:2005 number:6 day:04 month:08 pages:725-730 https://dx.doi.org/10.1007/s00217-005-0066-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 221 2005 6 04 08 725-730 |
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10.1007/s00217-005-0066-2 doi (DE-627)SPR002288303 (SPR)s00217-005-0066-2-e DE-627 ger DE-627 rakwb eng Liu, Yi-Ching verfasserin aut Detection of genetically modified soybean and its product tou-kan by polymerase chain reaction with dual pairs of DNA primers 2005 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag 2005 Abstract Since genetically modified (GM) crops and foods began to appear in market, the detection of these GM foods has become an important issue. Efficient and reliable methods are urgently needed to analyze the GM content of a large quantity of foods. However, most foods are processed through heating or cooking, and their proteins are denatured. Therefore, DNA rather than protein is the major target for detecting GM foods. In this report, polymerase chain reaction (PCR) was performed with dual sets of DNA primers, which co-amplified the soybean-specific lectin gene and the transgenic petunia transit peptide sequence (CTP) in the 5′ end of the 5-enol-pyruvyl-shikimate-3-phosphate synthase (EPSPS) gene within the same reaction. PCR with these two sets of primers reacted specifically with lectin gene and CTP, and can detect soybean with GM content less than 1%. PCR detecting both lectin gene and CTP was also applied to a soybean-derived product tou-kan, a traditional oriental food. Results indicated that although DNA was partially degraded in tou-kan, this PCR method was still able to detect the presence of EPSPS gene. However, when the DNA within tou-kan was destroyed badly, neither lectin nor EPSPS genes were detected by the PCR suggested here. Finally, an examination procedure for the Roundup Ready soybean was suggested according to the results of PCR with dual pairs of DNA primers. The proposed PCR method has proved to be a reliable and efficient method for detecting the GM content of the processed foods from Roundup Ready soybean. Genetically modified soybean (dpeaa)DE-He213 Tou-kan (dpeaa)DE-He213 Polymerase chain reaction (dpeaa)DE-He213 Lin, Shin-Huang aut Lai, Hsi-Mei aut Jeng, Shih-Tong aut Enthalten in European food research and technology Berlin : Springer, 1999 221(2005), 6 vom: 04. Aug., Seite 725-730 (DE-627)27012859X (DE-600)1476605-X 1438-2385 nnns volume:221 year:2005 number:6 day:04 month:08 pages:725-730 https://dx.doi.org/10.1007/s00217-005-0066-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 221 2005 6 04 08 725-730 |
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10.1007/s00217-005-0066-2 doi (DE-627)SPR002288303 (SPR)s00217-005-0066-2-e DE-627 ger DE-627 rakwb eng Liu, Yi-Ching verfasserin aut Detection of genetically modified soybean and its product tou-kan by polymerase chain reaction with dual pairs of DNA primers 2005 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag 2005 Abstract Since genetically modified (GM) crops and foods began to appear in market, the detection of these GM foods has become an important issue. Efficient and reliable methods are urgently needed to analyze the GM content of a large quantity of foods. However, most foods are processed through heating or cooking, and their proteins are denatured. Therefore, DNA rather than protein is the major target for detecting GM foods. In this report, polymerase chain reaction (PCR) was performed with dual sets of DNA primers, which co-amplified the soybean-specific lectin gene and the transgenic petunia transit peptide sequence (CTP) in the 5′ end of the 5-enol-pyruvyl-shikimate-3-phosphate synthase (EPSPS) gene within the same reaction. PCR with these two sets of primers reacted specifically with lectin gene and CTP, and can detect soybean with GM content less than 1%. PCR detecting both lectin gene and CTP was also applied to a soybean-derived product tou-kan, a traditional oriental food. Results indicated that although DNA was partially degraded in tou-kan, this PCR method was still able to detect the presence of EPSPS gene. However, when the DNA within tou-kan was destroyed badly, neither lectin nor EPSPS genes were detected by the PCR suggested here. Finally, an examination procedure for the Roundup Ready soybean was suggested according to the results of PCR with dual pairs of DNA primers. The proposed PCR method has proved to be a reliable and efficient method for detecting the GM content of the processed foods from Roundup Ready soybean. Genetically modified soybean (dpeaa)DE-He213 Tou-kan (dpeaa)DE-He213 Polymerase chain reaction (dpeaa)DE-He213 Lin, Shin-Huang aut Lai, Hsi-Mei aut Jeng, Shih-Tong aut Enthalten in European food research and technology Berlin : Springer, 1999 221(2005), 6 vom: 04. Aug., Seite 725-730 (DE-627)27012859X (DE-600)1476605-X 1438-2385 nnns volume:221 year:2005 number:6 day:04 month:08 pages:725-730 https://dx.doi.org/10.1007/s00217-005-0066-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 221 2005 6 04 08 725-730 |
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10.1007/s00217-005-0066-2 doi (DE-627)SPR002288303 (SPR)s00217-005-0066-2-e DE-627 ger DE-627 rakwb eng Liu, Yi-Ching verfasserin aut Detection of genetically modified soybean and its product tou-kan by polymerase chain reaction with dual pairs of DNA primers 2005 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag 2005 Abstract Since genetically modified (GM) crops and foods began to appear in market, the detection of these GM foods has become an important issue. Efficient and reliable methods are urgently needed to analyze the GM content of a large quantity of foods. However, most foods are processed through heating or cooking, and their proteins are denatured. Therefore, DNA rather than protein is the major target for detecting GM foods. In this report, polymerase chain reaction (PCR) was performed with dual sets of DNA primers, which co-amplified the soybean-specific lectin gene and the transgenic petunia transit peptide sequence (CTP) in the 5′ end of the 5-enol-pyruvyl-shikimate-3-phosphate synthase (EPSPS) gene within the same reaction. PCR with these two sets of primers reacted specifically with lectin gene and CTP, and can detect soybean with GM content less than 1%. PCR detecting both lectin gene and CTP was also applied to a soybean-derived product tou-kan, a traditional oriental food. Results indicated that although DNA was partially degraded in tou-kan, this PCR method was still able to detect the presence of EPSPS gene. However, when the DNA within tou-kan was destroyed badly, neither lectin nor EPSPS genes were detected by the PCR suggested here. Finally, an examination procedure for the Roundup Ready soybean was suggested according to the results of PCR with dual pairs of DNA primers. The proposed PCR method has proved to be a reliable and efficient method for detecting the GM content of the processed foods from Roundup Ready soybean. Genetically modified soybean (dpeaa)DE-He213 Tou-kan (dpeaa)DE-He213 Polymerase chain reaction (dpeaa)DE-He213 Lin, Shin-Huang aut Lai, Hsi-Mei aut Jeng, Shih-Tong aut Enthalten in European food research and technology Berlin : Springer, 1999 221(2005), 6 vom: 04. Aug., Seite 725-730 (DE-627)27012859X (DE-600)1476605-X 1438-2385 nnns volume:221 year:2005 number:6 day:04 month:08 pages:725-730 https://dx.doi.org/10.1007/s00217-005-0066-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 221 2005 6 04 08 725-730 |
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Enthalten in European food research and technology 221(2005), 6 vom: 04. Aug., Seite 725-730 volume:221 year:2005 number:6 day:04 month:08 pages:725-730 |
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Enthalten in European food research and technology 221(2005), 6 vom: 04. Aug., Seite 725-730 volume:221 year:2005 number:6 day:04 month:08 pages:725-730 |
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Genetically modified soybean Tou-kan Polymerase chain reaction |
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Liu, Yi-Ching @@aut@@ Lin, Shin-Huang @@aut@@ Lai, Hsi-Mei @@aut@@ Jeng, Shih-Tong @@aut@@ |
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Efficient and reliable methods are urgently needed to analyze the GM content of a large quantity of foods. However, most foods are processed through heating or cooking, and their proteins are denatured. Therefore, DNA rather than protein is the major target for detecting GM foods. In this report, polymerase chain reaction (PCR) was performed with dual sets of DNA primers, which co-amplified the soybean-specific lectin gene and the transgenic petunia transit peptide sequence (CTP) in the 5′ end of the 5-enol-pyruvyl-shikimate-3-phosphate synthase (EPSPS) gene within the same reaction. PCR with these two sets of primers reacted specifically with lectin gene and CTP, and can detect soybean with GM content less than 1%. PCR detecting both lectin gene and CTP was also applied to a soybean-derived product tou-kan, a traditional oriental food. Results indicated that although DNA was partially degraded in tou-kan, this PCR method was still able to detect the presence of EPSPS gene. However, when the DNA within tou-kan was destroyed badly, neither lectin nor EPSPS genes were detected by the PCR suggested here. Finally, an examination procedure for the Roundup Ready soybean was suggested according to the results of PCR with dual pairs of DNA primers. 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| author |
Liu, Yi-Ching |
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Liu, Yi-Ching misc Genetically modified soybean misc Tou-kan misc Polymerase chain reaction Detection of genetically modified soybean and its product tou-kan by polymerase chain reaction with dual pairs of DNA primers |
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Detection of genetically modified soybean and its product tou-kan by polymerase chain reaction with dual pairs of DNA primers Genetically modified soybean (dpeaa)DE-He213 Tou-kan (dpeaa)DE-He213 Polymerase chain reaction (dpeaa)DE-He213 |
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misc Genetically modified soybean misc Tou-kan misc Polymerase chain reaction |
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misc Genetically modified soybean misc Tou-kan misc Polymerase chain reaction |
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misc Genetically modified soybean misc Tou-kan misc Polymerase chain reaction |
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Detection of genetically modified soybean and its product tou-kan by polymerase chain reaction with dual pairs of DNA primers |
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Detection of genetically modified soybean and its product tou-kan by polymerase chain reaction with dual pairs of DNA primers |
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Liu, Yi-Ching |
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Liu, Yi-Ching Lin, Shin-Huang Lai, Hsi-Mei Jeng, Shih-Tong |
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10.1007/s00217-005-0066-2 |
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detection of genetically modified soybean and its product tou-kan by polymerase chain reaction with dual pairs of dna primers |
| title_auth |
Detection of genetically modified soybean and its product tou-kan by polymerase chain reaction with dual pairs of DNA primers |
| abstract |
Abstract Since genetically modified (GM) crops and foods began to appear in market, the detection of these GM foods has become an important issue. Efficient and reliable methods are urgently needed to analyze the GM content of a large quantity of foods. However, most foods are processed through heating or cooking, and their proteins are denatured. Therefore, DNA rather than protein is the major target for detecting GM foods. In this report, polymerase chain reaction (PCR) was performed with dual sets of DNA primers, which co-amplified the soybean-specific lectin gene and the transgenic petunia transit peptide sequence (CTP) in the 5′ end of the 5-enol-pyruvyl-shikimate-3-phosphate synthase (EPSPS) gene within the same reaction. PCR with these two sets of primers reacted specifically with lectin gene and CTP, and can detect soybean with GM content less than 1%. PCR detecting both lectin gene and CTP was also applied to a soybean-derived product tou-kan, a traditional oriental food. Results indicated that although DNA was partially degraded in tou-kan, this PCR method was still able to detect the presence of EPSPS gene. However, when the DNA within tou-kan was destroyed badly, neither lectin nor EPSPS genes were detected by the PCR suggested here. Finally, an examination procedure for the Roundup Ready soybean was suggested according to the results of PCR with dual pairs of DNA primers. The proposed PCR method has proved to be a reliable and efficient method for detecting the GM content of the processed foods from Roundup Ready soybean. © Springer-Verlag 2005 |
| abstractGer |
Abstract Since genetically modified (GM) crops and foods began to appear in market, the detection of these GM foods has become an important issue. Efficient and reliable methods are urgently needed to analyze the GM content of a large quantity of foods. However, most foods are processed through heating or cooking, and their proteins are denatured. Therefore, DNA rather than protein is the major target for detecting GM foods. In this report, polymerase chain reaction (PCR) was performed with dual sets of DNA primers, which co-amplified the soybean-specific lectin gene and the transgenic petunia transit peptide sequence (CTP) in the 5′ end of the 5-enol-pyruvyl-shikimate-3-phosphate synthase (EPSPS) gene within the same reaction. PCR with these two sets of primers reacted specifically with lectin gene and CTP, and can detect soybean with GM content less than 1%. PCR detecting both lectin gene and CTP was also applied to a soybean-derived product tou-kan, a traditional oriental food. Results indicated that although DNA was partially degraded in tou-kan, this PCR method was still able to detect the presence of EPSPS gene. However, when the DNA within tou-kan was destroyed badly, neither lectin nor EPSPS genes were detected by the PCR suggested here. Finally, an examination procedure for the Roundup Ready soybean was suggested according to the results of PCR with dual pairs of DNA primers. The proposed PCR method has proved to be a reliable and efficient method for detecting the GM content of the processed foods from Roundup Ready soybean. © Springer-Verlag 2005 |
| abstract_unstemmed |
Abstract Since genetically modified (GM) crops and foods began to appear in market, the detection of these GM foods has become an important issue. Efficient and reliable methods are urgently needed to analyze the GM content of a large quantity of foods. However, most foods are processed through heating or cooking, and their proteins are denatured. Therefore, DNA rather than protein is the major target for detecting GM foods. In this report, polymerase chain reaction (PCR) was performed with dual sets of DNA primers, which co-amplified the soybean-specific lectin gene and the transgenic petunia transit peptide sequence (CTP) in the 5′ end of the 5-enol-pyruvyl-shikimate-3-phosphate synthase (EPSPS) gene within the same reaction. PCR with these two sets of primers reacted specifically with lectin gene and CTP, and can detect soybean with GM content less than 1%. PCR detecting both lectin gene and CTP was also applied to a soybean-derived product tou-kan, a traditional oriental food. Results indicated that although DNA was partially degraded in tou-kan, this PCR method was still able to detect the presence of EPSPS gene. However, when the DNA within tou-kan was destroyed badly, neither lectin nor EPSPS genes were detected by the PCR suggested here. Finally, an examination procedure for the Roundup Ready soybean was suggested according to the results of PCR with dual pairs of DNA primers. The proposed PCR method has proved to be a reliable and efficient method for detecting the GM content of the processed foods from Roundup Ready soybean. © Springer-Verlag 2005 |
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Detection of genetically modified soybean and its product tou-kan by polymerase chain reaction with dual pairs of DNA primers |
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https://dx.doi.org/10.1007/s00217-005-0066-2 |
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Lin, Shin-Huang Lai, Hsi-Mei Jeng, Shih-Tong |
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10.1007/s00217-005-0066-2 |
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2024-07-04T02:30:09.133Z |
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| score |
7.401046 |