Separation and extraction of antimicrobial lipopeptides produced by Bacillus amyloliquefaciens ES-2 with macroporous resin
Abstract A new method for separation and extraction of antibiotic lipopeptides produced by Bacillus amyloliquefaciens ES-2 with macroporous resin was carried out in this study. Among widely used twelve macroporous resins, X-5 resin had the highest adsorption capacity in static and dynamic tests, its...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Wang, Yu [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2010 |
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| Schlagwörter: |
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| Anmerkung: |
© Springer-Verlag 2010 |
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| Übergeordnetes Werk: |
Enthalten in: European food research and technology - Berlin : Springer, 1999, 231(2010), 2 vom: 08. Apr., Seite 189-196 |
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| Übergeordnetes Werk: |
volume:231 ; year:2010 ; number:2 ; day:08 ; month:04 ; pages:189-196 |
| Links: |
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| DOI / URN: |
10.1007/s00217-010-1271-1 |
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SPR00230046X |
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| 100 | 1 | |a Wang, Yu |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Separation and extraction of antimicrobial lipopeptides produced by Bacillus amyloliquefaciens ES-2 with macroporous resin |
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| 520 | |a Abstract A new method for separation and extraction of antibiotic lipopeptides produced by Bacillus amyloliquefaciens ES-2 with macroporous resin was carried out in this study. Among widely used twelve macroporous resins, X-5 resin had the highest adsorption capacity in static and dynamic tests, its non-polarity and configuration were suited for the lipopeptides (surfactin and fengycin). Dynamic adsorption and desorption experiments were performed using a column packed with X-5 resin to obtain optimal conditions, with 30 g/L resin concentration, 0.5 BV/h feed flow rate, 80% aqueous ethanol at 1 BV/h elute flow rate as eluant agent, the total content of lipopeptides in final product was 79.5% (surfactin content was 33.2% while fengycin content was 23.4%), which had been improved about 150% compared with that of lipopeptide in fermentation broth. Because of its low cost, high efficiency, procedural simplicity and scale up ease, the adsorption–desorption method could be more efficient for the preparative separation and extraction of antibiotic lipopeptide than the conventional acid precipitation method, which could provide scientific references for antibiotic lipopeptide production. | ||
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| 650 | 4 | |a Macroporous resin |7 (dpeaa)DE-He213 | |
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| 700 | 1 | |a Lu, Zhaoxin |4 aut | |
| 700 | 1 | |a Bie, Xiaomei |4 aut | |
| 700 | 1 | |a Lv, Fengxia |4 aut | |
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10.1007/s00217-010-1271-1 doi (DE-627)SPR00230046X (SPR)s00217-010-1271-1-e DE-627 ger DE-627 rakwb eng Wang, Yu verfasserin aut Separation and extraction of antimicrobial lipopeptides produced by Bacillus amyloliquefaciens ES-2 with macroporous resin 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag 2010 Abstract A new method for separation and extraction of antibiotic lipopeptides produced by Bacillus amyloliquefaciens ES-2 with macroporous resin was carried out in this study. Among widely used twelve macroporous resins, X-5 resin had the highest adsorption capacity in static and dynamic tests, its non-polarity and configuration were suited for the lipopeptides (surfactin and fengycin). Dynamic adsorption and desorption experiments were performed using a column packed with X-5 resin to obtain optimal conditions, with 30 g/L resin concentration, 0.5 BV/h feed flow rate, 80% aqueous ethanol at 1 BV/h elute flow rate as eluant agent, the total content of lipopeptides in final product was 79.5% (surfactin content was 33.2% while fengycin content was 23.4%), which had been improved about 150% compared with that of lipopeptide in fermentation broth. Because of its low cost, high efficiency, procedural simplicity and scale up ease, the adsorption–desorption method could be more efficient for the preparative separation and extraction of antibiotic lipopeptide than the conventional acid precipitation method, which could provide scientific references for antibiotic lipopeptide production. Antibiotic lipopeptide (dpeaa)DE-He213 Macroporous resin (dpeaa)DE-He213 Separation·extraction (dpeaa)DE-He213 Lu, Zhaoxin aut Bie, Xiaomei aut Lv, Fengxia aut Enthalten in European food research and technology Berlin : Springer, 1999 231(2010), 2 vom: 08. Apr., Seite 189-196 (DE-627)27012859X (DE-600)1476605-X 1438-2385 nnns volume:231 year:2010 number:2 day:08 month:04 pages:189-196 https://dx.doi.org/10.1007/s00217-010-1271-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 231 2010 2 08 04 189-196 |
| spelling |
10.1007/s00217-010-1271-1 doi (DE-627)SPR00230046X (SPR)s00217-010-1271-1-e DE-627 ger DE-627 rakwb eng Wang, Yu verfasserin aut Separation and extraction of antimicrobial lipopeptides produced by Bacillus amyloliquefaciens ES-2 with macroporous resin 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag 2010 Abstract A new method for separation and extraction of antibiotic lipopeptides produced by Bacillus amyloliquefaciens ES-2 with macroporous resin was carried out in this study. Among widely used twelve macroporous resins, X-5 resin had the highest adsorption capacity in static and dynamic tests, its non-polarity and configuration were suited for the lipopeptides (surfactin and fengycin). Dynamic adsorption and desorption experiments were performed using a column packed with X-5 resin to obtain optimal conditions, with 30 g/L resin concentration, 0.5 BV/h feed flow rate, 80% aqueous ethanol at 1 BV/h elute flow rate as eluant agent, the total content of lipopeptides in final product was 79.5% (surfactin content was 33.2% while fengycin content was 23.4%), which had been improved about 150% compared with that of lipopeptide in fermentation broth. Because of its low cost, high efficiency, procedural simplicity and scale up ease, the adsorption–desorption method could be more efficient for the preparative separation and extraction of antibiotic lipopeptide than the conventional acid precipitation method, which could provide scientific references for antibiotic lipopeptide production. Antibiotic lipopeptide (dpeaa)DE-He213 Macroporous resin (dpeaa)DE-He213 Separation·extraction (dpeaa)DE-He213 Lu, Zhaoxin aut Bie, Xiaomei aut Lv, Fengxia aut Enthalten in European food research and technology Berlin : Springer, 1999 231(2010), 2 vom: 08. Apr., Seite 189-196 (DE-627)27012859X (DE-600)1476605-X 1438-2385 nnns volume:231 year:2010 number:2 day:08 month:04 pages:189-196 https://dx.doi.org/10.1007/s00217-010-1271-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 231 2010 2 08 04 189-196 |
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10.1007/s00217-010-1271-1 doi (DE-627)SPR00230046X (SPR)s00217-010-1271-1-e DE-627 ger DE-627 rakwb eng Wang, Yu verfasserin aut Separation and extraction of antimicrobial lipopeptides produced by Bacillus amyloliquefaciens ES-2 with macroporous resin 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag 2010 Abstract A new method for separation and extraction of antibiotic lipopeptides produced by Bacillus amyloliquefaciens ES-2 with macroporous resin was carried out in this study. Among widely used twelve macroporous resins, X-5 resin had the highest adsorption capacity in static and dynamic tests, its non-polarity and configuration were suited for the lipopeptides (surfactin and fengycin). Dynamic adsorption and desorption experiments were performed using a column packed with X-5 resin to obtain optimal conditions, with 30 g/L resin concentration, 0.5 BV/h feed flow rate, 80% aqueous ethanol at 1 BV/h elute flow rate as eluant agent, the total content of lipopeptides in final product was 79.5% (surfactin content was 33.2% while fengycin content was 23.4%), which had been improved about 150% compared with that of lipopeptide in fermentation broth. Because of its low cost, high efficiency, procedural simplicity and scale up ease, the adsorption–desorption method could be more efficient for the preparative separation and extraction of antibiotic lipopeptide than the conventional acid precipitation method, which could provide scientific references for antibiotic lipopeptide production. Antibiotic lipopeptide (dpeaa)DE-He213 Macroporous resin (dpeaa)DE-He213 Separation·extraction (dpeaa)DE-He213 Lu, Zhaoxin aut Bie, Xiaomei aut Lv, Fengxia aut Enthalten in European food research and technology Berlin : Springer, 1999 231(2010), 2 vom: 08. Apr., Seite 189-196 (DE-627)27012859X (DE-600)1476605-X 1438-2385 nnns volume:231 year:2010 number:2 day:08 month:04 pages:189-196 https://dx.doi.org/10.1007/s00217-010-1271-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 231 2010 2 08 04 189-196 |
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10.1007/s00217-010-1271-1 doi (DE-627)SPR00230046X (SPR)s00217-010-1271-1-e DE-627 ger DE-627 rakwb eng Wang, Yu verfasserin aut Separation and extraction of antimicrobial lipopeptides produced by Bacillus amyloliquefaciens ES-2 with macroporous resin 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag 2010 Abstract A new method for separation and extraction of antibiotic lipopeptides produced by Bacillus amyloliquefaciens ES-2 with macroporous resin was carried out in this study. Among widely used twelve macroporous resins, X-5 resin had the highest adsorption capacity in static and dynamic tests, its non-polarity and configuration were suited for the lipopeptides (surfactin and fengycin). Dynamic adsorption and desorption experiments were performed using a column packed with X-5 resin to obtain optimal conditions, with 30 g/L resin concentration, 0.5 BV/h feed flow rate, 80% aqueous ethanol at 1 BV/h elute flow rate as eluant agent, the total content of lipopeptides in final product was 79.5% (surfactin content was 33.2% while fengycin content was 23.4%), which had been improved about 150% compared with that of lipopeptide in fermentation broth. Because of its low cost, high efficiency, procedural simplicity and scale up ease, the adsorption–desorption method could be more efficient for the preparative separation and extraction of antibiotic lipopeptide than the conventional acid precipitation method, which could provide scientific references for antibiotic lipopeptide production. Antibiotic lipopeptide (dpeaa)DE-He213 Macroporous resin (dpeaa)DE-He213 Separation·extraction (dpeaa)DE-He213 Lu, Zhaoxin aut Bie, Xiaomei aut Lv, Fengxia aut Enthalten in European food research and technology Berlin : Springer, 1999 231(2010), 2 vom: 08. Apr., Seite 189-196 (DE-627)27012859X (DE-600)1476605-X 1438-2385 nnns volume:231 year:2010 number:2 day:08 month:04 pages:189-196 https://dx.doi.org/10.1007/s00217-010-1271-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 231 2010 2 08 04 189-196 |
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10.1007/s00217-010-1271-1 doi (DE-627)SPR00230046X (SPR)s00217-010-1271-1-e DE-627 ger DE-627 rakwb eng Wang, Yu verfasserin aut Separation and extraction of antimicrobial lipopeptides produced by Bacillus amyloliquefaciens ES-2 with macroporous resin 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag 2010 Abstract A new method for separation and extraction of antibiotic lipopeptides produced by Bacillus amyloliquefaciens ES-2 with macroporous resin was carried out in this study. Among widely used twelve macroporous resins, X-5 resin had the highest adsorption capacity in static and dynamic tests, its non-polarity and configuration were suited for the lipopeptides (surfactin and fengycin). Dynamic adsorption and desorption experiments were performed using a column packed with X-5 resin to obtain optimal conditions, with 30 g/L resin concentration, 0.5 BV/h feed flow rate, 80% aqueous ethanol at 1 BV/h elute flow rate as eluant agent, the total content of lipopeptides in final product was 79.5% (surfactin content was 33.2% while fengycin content was 23.4%), which had been improved about 150% compared with that of lipopeptide in fermentation broth. Because of its low cost, high efficiency, procedural simplicity and scale up ease, the adsorption–desorption method could be more efficient for the preparative separation and extraction of antibiotic lipopeptide than the conventional acid precipitation method, which could provide scientific references for antibiotic lipopeptide production. Antibiotic lipopeptide (dpeaa)DE-He213 Macroporous resin (dpeaa)DE-He213 Separation·extraction (dpeaa)DE-He213 Lu, Zhaoxin aut Bie, Xiaomei aut Lv, Fengxia aut Enthalten in European food research and technology Berlin : Springer, 1999 231(2010), 2 vom: 08. Apr., Seite 189-196 (DE-627)27012859X (DE-600)1476605-X 1438-2385 nnns volume:231 year:2010 number:2 day:08 month:04 pages:189-196 https://dx.doi.org/10.1007/s00217-010-1271-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 231 2010 2 08 04 189-196 |
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Enthalten in European food research and technology 231(2010), 2 vom: 08. Apr., Seite 189-196 volume:231 year:2010 number:2 day:08 month:04 pages:189-196 |
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Enthalten in European food research and technology 231(2010), 2 vom: 08. Apr., Seite 189-196 volume:231 year:2010 number:2 day:08 month:04 pages:189-196 |
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Wang, Yu @@aut@@ Lu, Zhaoxin @@aut@@ Bie, Xiaomei @@aut@@ Lv, Fengxia @@aut@@ |
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Wang, Yu |
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Separation and extraction of antimicrobial lipopeptides produced by Bacillus amyloliquefaciens ES-2 with macroporous resin Antibiotic lipopeptide (dpeaa)DE-He213 Macroporous resin (dpeaa)DE-He213 Separation·extraction (dpeaa)DE-He213 |
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separation and extraction of antimicrobial lipopeptides produced by bacillus amyloliquefaciens es-2 with macroporous resin |
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Separation and extraction of antimicrobial lipopeptides produced by Bacillus amyloliquefaciens ES-2 with macroporous resin |
| abstract |
Abstract A new method for separation and extraction of antibiotic lipopeptides produced by Bacillus amyloliquefaciens ES-2 with macroporous resin was carried out in this study. Among widely used twelve macroporous resins, X-5 resin had the highest adsorption capacity in static and dynamic tests, its non-polarity and configuration were suited for the lipopeptides (surfactin and fengycin). Dynamic adsorption and desorption experiments were performed using a column packed with X-5 resin to obtain optimal conditions, with 30 g/L resin concentration, 0.5 BV/h feed flow rate, 80% aqueous ethanol at 1 BV/h elute flow rate as eluant agent, the total content of lipopeptides in final product was 79.5% (surfactin content was 33.2% while fengycin content was 23.4%), which had been improved about 150% compared with that of lipopeptide in fermentation broth. Because of its low cost, high efficiency, procedural simplicity and scale up ease, the adsorption–desorption method could be more efficient for the preparative separation and extraction of antibiotic lipopeptide than the conventional acid precipitation method, which could provide scientific references for antibiotic lipopeptide production. © Springer-Verlag 2010 |
| abstractGer |
Abstract A new method for separation and extraction of antibiotic lipopeptides produced by Bacillus amyloliquefaciens ES-2 with macroporous resin was carried out in this study. Among widely used twelve macroporous resins, X-5 resin had the highest adsorption capacity in static and dynamic tests, its non-polarity and configuration were suited for the lipopeptides (surfactin and fengycin). Dynamic adsorption and desorption experiments were performed using a column packed with X-5 resin to obtain optimal conditions, with 30 g/L resin concentration, 0.5 BV/h feed flow rate, 80% aqueous ethanol at 1 BV/h elute flow rate as eluant agent, the total content of lipopeptides in final product was 79.5% (surfactin content was 33.2% while fengycin content was 23.4%), which had been improved about 150% compared with that of lipopeptide in fermentation broth. Because of its low cost, high efficiency, procedural simplicity and scale up ease, the adsorption–desorption method could be more efficient for the preparative separation and extraction of antibiotic lipopeptide than the conventional acid precipitation method, which could provide scientific references for antibiotic lipopeptide production. © Springer-Verlag 2010 |
| abstract_unstemmed |
Abstract A new method for separation and extraction of antibiotic lipopeptides produced by Bacillus amyloliquefaciens ES-2 with macroporous resin was carried out in this study. Among widely used twelve macroporous resins, X-5 resin had the highest adsorption capacity in static and dynamic tests, its non-polarity and configuration were suited for the lipopeptides (surfactin and fengycin). Dynamic adsorption and desorption experiments were performed using a column packed with X-5 resin to obtain optimal conditions, with 30 g/L resin concentration, 0.5 BV/h feed flow rate, 80% aqueous ethanol at 1 BV/h elute flow rate as eluant agent, the total content of lipopeptides in final product was 79.5% (surfactin content was 33.2% while fengycin content was 23.4%), which had been improved about 150% compared with that of lipopeptide in fermentation broth. Because of its low cost, high efficiency, procedural simplicity and scale up ease, the adsorption–desorption method could be more efficient for the preparative separation and extraction of antibiotic lipopeptide than the conventional acid precipitation method, which could provide scientific references for antibiotic lipopeptide production. © Springer-Verlag 2010 |
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Separation and extraction of antimicrobial lipopeptides produced by Bacillus amyloliquefaciens ES-2 with macroporous resin |
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https://dx.doi.org/10.1007/s00217-010-1271-1 |
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Lu, Zhaoxin Bie, Xiaomei Lv, Fengxia |
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Lu, Zhaoxin Bie, Xiaomei Lv, Fengxia |
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10.1007/s00217-010-1271-1 |
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2024-07-04T02:32:52.157Z |
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