Intracellular pH homeostasis plays a role in the NaCl tolerance of Debaryomyces hansenii strains
Abstract The effects of NaCl stress on cell area and intracellular pH ($ pH_{i} $) of individual cells of two Debaryomyces hansenii strains were investigated. Our results show that one of the strains was more NaCl tolerant than the other, as determined by the rate of growth initiation. Whereas NaCl...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Mortensen, H. D. [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2005 |
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| Schlagwörter: |
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| Anmerkung: |
© Springer-Verlag 2005 |
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| Übergeordnetes Werk: |
Enthalten in: Applied microbiology and biotechnology - Berlin : Springer, 1975, 71(2005), 5 vom: 21. Okt., Seite 713-719 |
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| Übergeordnetes Werk: |
volume:71 ; year:2005 ; number:5 ; day:21 ; month:10 ; pages:713-719 |
| Links: |
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| DOI / URN: |
10.1007/s00253-005-0196-2 |
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| Katalog-ID: |
SPR002941295 |
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| 100 | 1 | |a Mortensen, H. D. |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Intracellular pH homeostasis plays a role in the NaCl tolerance of Debaryomyces hansenii strains |
| 264 | 1 | |c 2005 | |
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| 520 | |a Abstract The effects of NaCl stress on cell area and intracellular pH ($ pH_{i} $) of individual cells of two Debaryomyces hansenii strains were investigated. Our results show that one of the strains was more NaCl tolerant than the other, as determined by the rate of growth initiation. Whereas NaCl stress caused similar cell shrinkages (30–35%), it caused different $ pH_{i} $ changes of the two D. hansenii strains; i.e., in the more NaCl-tolerant strain, $ pH_{i} $ homeostasis was maintained, whereas in the less NaCl-tolerant strain, intracellular acidification occurred. Thus, cell shrinkage could not explain the different intracellular acidifications in the two strains. Instead, we introduce the concept of yeasts having an intracellular $ pK_{a} $ ($ pK_{a,i} $) value, since permeabilized D. hansenii cells had a very high buffer capacity at a certain pH. Our results demonstrate that the more NaCl-tolerant strain was better able to maintain its $ pK_{a,i} $ close to its $ pH_{i} $ homeostasis level during NaCl stress. In turn, these findings indicate that the closer a D. hansenii strain can keep its $ pK_{a,i} $ to its $ pH_{i} $ homeostasis level, the better it may manage NaCl stress. Furthermore, our results suggest that the NaCl-induced effects on $ pH_{i} $ were mainly due to hyperosmotic stress and not ionic stress. | ||
| 650 | 4 | |a Hyperosmotic Stress |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Debaryomyces Hansenii |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Late Exponential Growth Phase |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a High Buffer Capacity |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Hansenii Strain |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Gori, K. |4 aut | |
| 700 | 1 | |a Siegumfeldt, H. |4 aut | |
| 700 | 1 | |a Nissen, P. |4 aut | |
| 700 | 1 | |a Jespersen, L. |4 aut | |
| 700 | 1 | |a Arneborg, N. |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Applied microbiology and biotechnology |d Berlin : Springer, 1975 |g 71(2005), 5 vom: 21. Okt., Seite 713-719 |w (DE-627)265509564 |w (DE-600)1464336-4 |x 1432-0614 |7 nnns |
| 773 | 1 | 8 | |g volume:71 |g year:2005 |g number:5 |g day:21 |g month:10 |g pages:713-719 |
| 856 | 4 | 0 | |u https://dx.doi.org/10.1007/s00253-005-0196-2 |z lizenzpflichtig |3 Volltext |
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10.1007/s00253-005-0196-2 doi (DE-627)SPR002941295 (SPR)s00253-005-0196-2-e DE-627 ger DE-627 rakwb eng Mortensen, H. D. verfasserin aut Intracellular pH homeostasis plays a role in the NaCl tolerance of Debaryomyces hansenii strains 2005 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag 2005 Abstract The effects of NaCl stress on cell area and intracellular pH ($ pH_{i} $) of individual cells of two Debaryomyces hansenii strains were investigated. Our results show that one of the strains was more NaCl tolerant than the other, as determined by the rate of growth initiation. Whereas NaCl stress caused similar cell shrinkages (30–35%), it caused different $ pH_{i} $ changes of the two D. hansenii strains; i.e., in the more NaCl-tolerant strain, $ pH_{i} $ homeostasis was maintained, whereas in the less NaCl-tolerant strain, intracellular acidification occurred. Thus, cell shrinkage could not explain the different intracellular acidifications in the two strains. Instead, we introduce the concept of yeasts having an intracellular $ pK_{a} $ ($ pK_{a,i} $) value, since permeabilized D. hansenii cells had a very high buffer capacity at a certain pH. Our results demonstrate that the more NaCl-tolerant strain was better able to maintain its $ pK_{a,i} $ close to its $ pH_{i} $ homeostasis level during NaCl stress. In turn, these findings indicate that the closer a D. hansenii strain can keep its $ pK_{a,i} $ to its $ pH_{i} $ homeostasis level, the better it may manage NaCl stress. Furthermore, our results suggest that the NaCl-induced effects on $ pH_{i} $ were mainly due to hyperosmotic stress and not ionic stress. Hyperosmotic Stress (dpeaa)DE-He213 Debaryomyces Hansenii (dpeaa)DE-He213 Late Exponential Growth Phase (dpeaa)DE-He213 High Buffer Capacity (dpeaa)DE-He213 Hansenii Strain (dpeaa)DE-He213 Gori, K. aut Siegumfeldt, H. aut Nissen, P. aut Jespersen, L. aut Arneborg, N. aut Enthalten in Applied microbiology and biotechnology Berlin : Springer, 1975 71(2005), 5 vom: 21. Okt., Seite 713-719 (DE-627)265509564 (DE-600)1464336-4 1432-0614 nnns volume:71 year:2005 number:5 day:21 month:10 pages:713-719 https://dx.doi.org/10.1007/s00253-005-0196-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2110 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 71 2005 5 21 10 713-719 |
| spelling |
10.1007/s00253-005-0196-2 doi (DE-627)SPR002941295 (SPR)s00253-005-0196-2-e DE-627 ger DE-627 rakwb eng Mortensen, H. D. verfasserin aut Intracellular pH homeostasis plays a role in the NaCl tolerance of Debaryomyces hansenii strains 2005 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag 2005 Abstract The effects of NaCl stress on cell area and intracellular pH ($ pH_{i} $) of individual cells of two Debaryomyces hansenii strains were investigated. Our results show that one of the strains was more NaCl tolerant than the other, as determined by the rate of growth initiation. Whereas NaCl stress caused similar cell shrinkages (30–35%), it caused different $ pH_{i} $ changes of the two D. hansenii strains; i.e., in the more NaCl-tolerant strain, $ pH_{i} $ homeostasis was maintained, whereas in the less NaCl-tolerant strain, intracellular acidification occurred. Thus, cell shrinkage could not explain the different intracellular acidifications in the two strains. Instead, we introduce the concept of yeasts having an intracellular $ pK_{a} $ ($ pK_{a,i} $) value, since permeabilized D. hansenii cells had a very high buffer capacity at a certain pH. Our results demonstrate that the more NaCl-tolerant strain was better able to maintain its $ pK_{a,i} $ close to its $ pH_{i} $ homeostasis level during NaCl stress. In turn, these findings indicate that the closer a D. hansenii strain can keep its $ pK_{a,i} $ to its $ pH_{i} $ homeostasis level, the better it may manage NaCl stress. Furthermore, our results suggest that the NaCl-induced effects on $ pH_{i} $ were mainly due to hyperosmotic stress and not ionic stress. Hyperosmotic Stress (dpeaa)DE-He213 Debaryomyces Hansenii (dpeaa)DE-He213 Late Exponential Growth Phase (dpeaa)DE-He213 High Buffer Capacity (dpeaa)DE-He213 Hansenii Strain (dpeaa)DE-He213 Gori, K. aut Siegumfeldt, H. aut Nissen, P. aut Jespersen, L. aut Arneborg, N. aut Enthalten in Applied microbiology and biotechnology Berlin : Springer, 1975 71(2005), 5 vom: 21. Okt., Seite 713-719 (DE-627)265509564 (DE-600)1464336-4 1432-0614 nnns volume:71 year:2005 number:5 day:21 month:10 pages:713-719 https://dx.doi.org/10.1007/s00253-005-0196-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2110 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 71 2005 5 21 10 713-719 |
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10.1007/s00253-005-0196-2 doi (DE-627)SPR002941295 (SPR)s00253-005-0196-2-e DE-627 ger DE-627 rakwb eng Mortensen, H. D. verfasserin aut Intracellular pH homeostasis plays a role in the NaCl tolerance of Debaryomyces hansenii strains 2005 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag 2005 Abstract The effects of NaCl stress on cell area and intracellular pH ($ pH_{i} $) of individual cells of two Debaryomyces hansenii strains were investigated. Our results show that one of the strains was more NaCl tolerant than the other, as determined by the rate of growth initiation. Whereas NaCl stress caused similar cell shrinkages (30–35%), it caused different $ pH_{i} $ changes of the two D. hansenii strains; i.e., in the more NaCl-tolerant strain, $ pH_{i} $ homeostasis was maintained, whereas in the less NaCl-tolerant strain, intracellular acidification occurred. Thus, cell shrinkage could not explain the different intracellular acidifications in the two strains. Instead, we introduce the concept of yeasts having an intracellular $ pK_{a} $ ($ pK_{a,i} $) value, since permeabilized D. hansenii cells had a very high buffer capacity at a certain pH. Our results demonstrate that the more NaCl-tolerant strain was better able to maintain its $ pK_{a,i} $ close to its $ pH_{i} $ homeostasis level during NaCl stress. In turn, these findings indicate that the closer a D. hansenii strain can keep its $ pK_{a,i} $ to its $ pH_{i} $ homeostasis level, the better it may manage NaCl stress. Furthermore, our results suggest that the NaCl-induced effects on $ pH_{i} $ were mainly due to hyperosmotic stress and not ionic stress. Hyperosmotic Stress (dpeaa)DE-He213 Debaryomyces Hansenii (dpeaa)DE-He213 Late Exponential Growth Phase (dpeaa)DE-He213 High Buffer Capacity (dpeaa)DE-He213 Hansenii Strain (dpeaa)DE-He213 Gori, K. aut Siegumfeldt, H. aut Nissen, P. aut Jespersen, L. aut Arneborg, N. aut Enthalten in Applied microbiology and biotechnology Berlin : Springer, 1975 71(2005), 5 vom: 21. Okt., Seite 713-719 (DE-627)265509564 (DE-600)1464336-4 1432-0614 nnns volume:71 year:2005 number:5 day:21 month:10 pages:713-719 https://dx.doi.org/10.1007/s00253-005-0196-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2110 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 71 2005 5 21 10 713-719 |
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10.1007/s00253-005-0196-2 doi (DE-627)SPR002941295 (SPR)s00253-005-0196-2-e DE-627 ger DE-627 rakwb eng Mortensen, H. D. verfasserin aut Intracellular pH homeostasis plays a role in the NaCl tolerance of Debaryomyces hansenii strains 2005 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag 2005 Abstract The effects of NaCl stress on cell area and intracellular pH ($ pH_{i} $) of individual cells of two Debaryomyces hansenii strains were investigated. Our results show that one of the strains was more NaCl tolerant than the other, as determined by the rate of growth initiation. Whereas NaCl stress caused similar cell shrinkages (30–35%), it caused different $ pH_{i} $ changes of the two D. hansenii strains; i.e., in the more NaCl-tolerant strain, $ pH_{i} $ homeostasis was maintained, whereas in the less NaCl-tolerant strain, intracellular acidification occurred. Thus, cell shrinkage could not explain the different intracellular acidifications in the two strains. Instead, we introduce the concept of yeasts having an intracellular $ pK_{a} $ ($ pK_{a,i} $) value, since permeabilized D. hansenii cells had a very high buffer capacity at a certain pH. Our results demonstrate that the more NaCl-tolerant strain was better able to maintain its $ pK_{a,i} $ close to its $ pH_{i} $ homeostasis level during NaCl stress. In turn, these findings indicate that the closer a D. hansenii strain can keep its $ pK_{a,i} $ to its $ pH_{i} $ homeostasis level, the better it may manage NaCl stress. Furthermore, our results suggest that the NaCl-induced effects on $ pH_{i} $ were mainly due to hyperosmotic stress and not ionic stress. Hyperosmotic Stress (dpeaa)DE-He213 Debaryomyces Hansenii (dpeaa)DE-He213 Late Exponential Growth Phase (dpeaa)DE-He213 High Buffer Capacity (dpeaa)DE-He213 Hansenii Strain (dpeaa)DE-He213 Gori, K. aut Siegumfeldt, H. aut Nissen, P. aut Jespersen, L. aut Arneborg, N. aut Enthalten in Applied microbiology and biotechnology Berlin : Springer, 1975 71(2005), 5 vom: 21. Okt., Seite 713-719 (DE-627)265509564 (DE-600)1464336-4 1432-0614 nnns volume:71 year:2005 number:5 day:21 month:10 pages:713-719 https://dx.doi.org/10.1007/s00253-005-0196-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2110 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 71 2005 5 21 10 713-719 |
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10.1007/s00253-005-0196-2 doi (DE-627)SPR002941295 (SPR)s00253-005-0196-2-e DE-627 ger DE-627 rakwb eng Mortensen, H. D. verfasserin aut Intracellular pH homeostasis plays a role in the NaCl tolerance of Debaryomyces hansenii strains 2005 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag 2005 Abstract The effects of NaCl stress on cell area and intracellular pH ($ pH_{i} $) of individual cells of two Debaryomyces hansenii strains were investigated. Our results show that one of the strains was more NaCl tolerant than the other, as determined by the rate of growth initiation. Whereas NaCl stress caused similar cell shrinkages (30–35%), it caused different $ pH_{i} $ changes of the two D. hansenii strains; i.e., in the more NaCl-tolerant strain, $ pH_{i} $ homeostasis was maintained, whereas in the less NaCl-tolerant strain, intracellular acidification occurred. Thus, cell shrinkage could not explain the different intracellular acidifications in the two strains. Instead, we introduce the concept of yeasts having an intracellular $ pK_{a} $ ($ pK_{a,i} $) value, since permeabilized D. hansenii cells had a very high buffer capacity at a certain pH. Our results demonstrate that the more NaCl-tolerant strain was better able to maintain its $ pK_{a,i} $ close to its $ pH_{i} $ homeostasis level during NaCl stress. In turn, these findings indicate that the closer a D. hansenii strain can keep its $ pK_{a,i} $ to its $ pH_{i} $ homeostasis level, the better it may manage NaCl stress. Furthermore, our results suggest that the NaCl-induced effects on $ pH_{i} $ were mainly due to hyperosmotic stress and not ionic stress. Hyperosmotic Stress (dpeaa)DE-He213 Debaryomyces Hansenii (dpeaa)DE-He213 Late Exponential Growth Phase (dpeaa)DE-He213 High Buffer Capacity (dpeaa)DE-He213 Hansenii Strain (dpeaa)DE-He213 Gori, K. aut Siegumfeldt, H. aut Nissen, P. aut Jespersen, L. aut Arneborg, N. aut Enthalten in Applied microbiology and biotechnology Berlin : Springer, 1975 71(2005), 5 vom: 21. Okt., Seite 713-719 (DE-627)265509564 (DE-600)1464336-4 1432-0614 nnns volume:71 year:2005 number:5 day:21 month:10 pages:713-719 https://dx.doi.org/10.1007/s00253-005-0196-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2110 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 71 2005 5 21 10 713-719 |
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Enthalten in Applied microbiology and biotechnology 71(2005), 5 vom: 21. Okt., Seite 713-719 volume:71 year:2005 number:5 day:21 month:10 pages:713-719 |
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Enthalten in Applied microbiology and biotechnology 71(2005), 5 vom: 21. Okt., Seite 713-719 volume:71 year:2005 number:5 day:21 month:10 pages:713-719 |
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Hyperosmotic Stress Debaryomyces Hansenii Late Exponential Growth Phase High Buffer Capacity Hansenii Strain |
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Mortensen, H. D. @@aut@@ Gori, K. @@aut@@ Siegumfeldt, H. @@aut@@ Nissen, P. @@aut@@ Jespersen, L. @@aut@@ Arneborg, N. @@aut@@ |
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D.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Intracellular pH homeostasis plays a role in the NaCl tolerance of Debaryomyces hansenii strains</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2005</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">© Springer-Verlag 2005</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract The effects of NaCl stress on cell area and intracellular pH ($ pH_{i} $) of individual cells of two Debaryomyces hansenii strains were investigated. Our results show that one of the strains was more NaCl tolerant than the other, as determined by the rate of growth initiation. Whereas NaCl stress caused similar cell shrinkages (30–35%), it caused different $ pH_{i} $ changes of the two D. hansenii strains; i.e., in the more NaCl-tolerant strain, $ pH_{i} $ homeostasis was maintained, whereas in the less NaCl-tolerant strain, intracellular acidification occurred. Thus, cell shrinkage could not explain the different intracellular acidifications in the two strains. Instead, we introduce the concept of yeasts having an intracellular $ pK_{a} $ ($ pK_{a,i} $) value, since permeabilized D. hansenii cells had a very high buffer capacity at a certain pH. Our results demonstrate that the more NaCl-tolerant strain was better able to maintain its $ pK_{a,i} $ close to its $ pH_{i} $ homeostasis level during NaCl stress. In turn, these findings indicate that the closer a D. hansenii strain can keep its $ pK_{a,i} $ to its $ pH_{i} $ homeostasis level, the better it may manage NaCl stress. 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Mortensen, H. D. |
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Mortensen, H. D. misc Hyperosmotic Stress misc Debaryomyces Hansenii misc Late Exponential Growth Phase misc High Buffer Capacity misc Hansenii Strain Intracellular pH homeostasis plays a role in the NaCl tolerance of Debaryomyces hansenii strains |
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Intracellular pH homeostasis plays a role in the NaCl tolerance of Debaryomyces hansenii strains Hyperosmotic Stress (dpeaa)DE-He213 Debaryomyces Hansenii (dpeaa)DE-He213 Late Exponential Growth Phase (dpeaa)DE-He213 High Buffer Capacity (dpeaa)DE-He213 Hansenii Strain (dpeaa)DE-He213 |
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intracellular ph homeostasis plays a role in the nacl tolerance of debaryomyces hansenii strains |
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Intracellular pH homeostasis plays a role in the NaCl tolerance of Debaryomyces hansenii strains |
| abstract |
Abstract The effects of NaCl stress on cell area and intracellular pH ($ pH_{i} $) of individual cells of two Debaryomyces hansenii strains were investigated. Our results show that one of the strains was more NaCl tolerant than the other, as determined by the rate of growth initiation. Whereas NaCl stress caused similar cell shrinkages (30–35%), it caused different $ pH_{i} $ changes of the two D. hansenii strains; i.e., in the more NaCl-tolerant strain, $ pH_{i} $ homeostasis was maintained, whereas in the less NaCl-tolerant strain, intracellular acidification occurred. Thus, cell shrinkage could not explain the different intracellular acidifications in the two strains. Instead, we introduce the concept of yeasts having an intracellular $ pK_{a} $ ($ pK_{a,i} $) value, since permeabilized D. hansenii cells had a very high buffer capacity at a certain pH. Our results demonstrate that the more NaCl-tolerant strain was better able to maintain its $ pK_{a,i} $ close to its $ pH_{i} $ homeostasis level during NaCl stress. In turn, these findings indicate that the closer a D. hansenii strain can keep its $ pK_{a,i} $ to its $ pH_{i} $ homeostasis level, the better it may manage NaCl stress. Furthermore, our results suggest that the NaCl-induced effects on $ pH_{i} $ were mainly due to hyperosmotic stress and not ionic stress. © Springer-Verlag 2005 |
| abstractGer |
Abstract The effects of NaCl stress on cell area and intracellular pH ($ pH_{i} $) of individual cells of two Debaryomyces hansenii strains were investigated. Our results show that one of the strains was more NaCl tolerant than the other, as determined by the rate of growth initiation. Whereas NaCl stress caused similar cell shrinkages (30–35%), it caused different $ pH_{i} $ changes of the two D. hansenii strains; i.e., in the more NaCl-tolerant strain, $ pH_{i} $ homeostasis was maintained, whereas in the less NaCl-tolerant strain, intracellular acidification occurred. Thus, cell shrinkage could not explain the different intracellular acidifications in the two strains. Instead, we introduce the concept of yeasts having an intracellular $ pK_{a} $ ($ pK_{a,i} $) value, since permeabilized D. hansenii cells had a very high buffer capacity at a certain pH. Our results demonstrate that the more NaCl-tolerant strain was better able to maintain its $ pK_{a,i} $ close to its $ pH_{i} $ homeostasis level during NaCl stress. In turn, these findings indicate that the closer a D. hansenii strain can keep its $ pK_{a,i} $ to its $ pH_{i} $ homeostasis level, the better it may manage NaCl stress. Furthermore, our results suggest that the NaCl-induced effects on $ pH_{i} $ were mainly due to hyperosmotic stress and not ionic stress. © Springer-Verlag 2005 |
| abstract_unstemmed |
Abstract The effects of NaCl stress on cell area and intracellular pH ($ pH_{i} $) of individual cells of two Debaryomyces hansenii strains were investigated. Our results show that one of the strains was more NaCl tolerant than the other, as determined by the rate of growth initiation. Whereas NaCl stress caused similar cell shrinkages (30–35%), it caused different $ pH_{i} $ changes of the two D. hansenii strains; i.e., in the more NaCl-tolerant strain, $ pH_{i} $ homeostasis was maintained, whereas in the less NaCl-tolerant strain, intracellular acidification occurred. Thus, cell shrinkage could not explain the different intracellular acidifications in the two strains. Instead, we introduce the concept of yeasts having an intracellular $ pK_{a} $ ($ pK_{a,i} $) value, since permeabilized D. hansenii cells had a very high buffer capacity at a certain pH. Our results demonstrate that the more NaCl-tolerant strain was better able to maintain its $ pK_{a,i} $ close to its $ pH_{i} $ homeostasis level during NaCl stress. In turn, these findings indicate that the closer a D. hansenii strain can keep its $ pK_{a,i} $ to its $ pH_{i} $ homeostasis level, the better it may manage NaCl stress. Furthermore, our results suggest that the NaCl-induced effects on $ pH_{i} $ were mainly due to hyperosmotic stress and not ionic stress. © Springer-Verlag 2005 |
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Intracellular pH homeostasis plays a role in the NaCl tolerance of Debaryomyces hansenii strains |
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Gori, K. Siegumfeldt, H. Nissen, P. Jespersen, L. Arneborg, N. |
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| score |
7.400216 |