Development and application of real-time PCR for quantification of specific ammonia-oxidizing bacteria in activated sludge of sewage treatment systems
Abstract In this study, four real-time polymerase chain reaction (PCR) primer sets were developed for the 16S rRNA genes of specific ammonia-oxidizing bacteria (AOB) found in activated sludge of sewage treatment systems. The primer sets target two of several sequence types of the Nitrosomonas oligot...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Limpiyakorn, Tawan [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2006 |
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| Schlagwörter: |
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| Anmerkung: |
© Springer-Verlag 2006 |
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| Übergeordnetes Werk: |
Enthalten in: Applied microbiology and biotechnology - Berlin : Springer, 1975, 72(2006), 5 vom: 08. März, Seite 1004-1013 |
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| Übergeordnetes Werk: |
volume:72 ; year:2006 ; number:5 ; day:08 ; month:03 ; pages:1004-1013 |
| Links: |
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| DOI / URN: |
10.1007/s00253-006-0366-x |
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| Katalog-ID: |
SPR002942909 |
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| 100 | 1 | |a Limpiyakorn, Tawan |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Development and application of real-time PCR for quantification of specific ammonia-oxidizing bacteria in activated sludge of sewage treatment systems |
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| 520 | |a Abstract In this study, four real-time polymerase chain reaction (PCR) primer sets were developed for the 16S rRNA genes of specific ammonia-oxidizing bacteria (AOB) found in activated sludge of sewage treatment systems. The primer sets target two of several sequence types of the Nitrosomonas oligotropha cluster, members within the Nitrosomonas communis cluster, and all members of the Nitrosomonas europaea–Nitrosococcus mobilis cluster. The detection limit of each primer set was in the range of 3×$ 10^{1} $–6×$ 10^{2} $ genes $ reaction^{−1} $. Reliable quantification of the target AOB DNA was obtained when the target AOB DNA comprised more than 0.1% of total AOB DNA in the sample. The application of the primer sets to samples taken from five sewage treatment systems showed that, in all systems, the majority of the AOB population was comprised of one sequence type of the N. oligotropha cluster (3.9±1.5×$ 10^{9} $–1.7±0.5×$ 10^{10} $ cell $ l^{−1} $) and, in most systems, followed by members within the N. communis cluster (2.8±0.3×$ 10^{9} $–1.0±0.1×$ 10^{10} $ cell $ l^{−1} $) or/and another sequence type of the N. oligotropha cluster (1.5±0.6×$ 10^{8} $–5.5±0.5×$ 10^{8} $ cell $ l^{−1} $). N. europaea–N. mobilis cluster arose solely in small numbers (4.9±0.8×$ 10^{8} $ cell $ l^{−1} $) in one system. Real-time PCR-amplified products obtained from genomic DNA extracted from samples were verified using clone library, and it revealed that only the target AOB DNA were PCR amplified, without amplification of the nontarget sequences. | ||
| 650 | 4 | |a Activate Sludge |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Clone Library |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a amoA Gene |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Mixed Liquor Suspended Solid |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Sewage Treatment System |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Kurisu, Futoshi |4 aut | |
| 700 | 1 | |a Yagi, Osami |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Applied microbiology and biotechnology |d Berlin : Springer, 1975 |g 72(2006), 5 vom: 08. März, Seite 1004-1013 |w (DE-627)265509564 |w (DE-600)1464336-4 |x 1432-0614 |7 nnns |
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10.1007/s00253-006-0366-x doi (DE-627)SPR002942909 (SPR)s00253-006-0366-x-e DE-627 ger DE-627 rakwb eng Limpiyakorn, Tawan verfasserin aut Development and application of real-time PCR for quantification of specific ammonia-oxidizing bacteria in activated sludge of sewage treatment systems 2006 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag 2006 Abstract In this study, four real-time polymerase chain reaction (PCR) primer sets were developed for the 16S rRNA genes of specific ammonia-oxidizing bacteria (AOB) found in activated sludge of sewage treatment systems. The primer sets target two of several sequence types of the Nitrosomonas oligotropha cluster, members within the Nitrosomonas communis cluster, and all members of the Nitrosomonas europaea–Nitrosococcus mobilis cluster. The detection limit of each primer set was in the range of 3×$ 10^{1} $–6×$ 10^{2} $ genes $ reaction^{−1} $. Reliable quantification of the target AOB DNA was obtained when the target AOB DNA comprised more than 0.1% of total AOB DNA in the sample. The application of the primer sets to samples taken from five sewage treatment systems showed that, in all systems, the majority of the AOB population was comprised of one sequence type of the N. oligotropha cluster (3.9±1.5×$ 10^{9} $–1.7±0.5×$ 10^{10} $ cell $ l^{−1} $) and, in most systems, followed by members within the N. communis cluster (2.8±0.3×$ 10^{9} $–1.0±0.1×$ 10^{10} $ cell $ l^{−1} $) or/and another sequence type of the N. oligotropha cluster (1.5±0.6×$ 10^{8} $–5.5±0.5×$ 10^{8} $ cell $ l^{−1} $). N. europaea–N. mobilis cluster arose solely in small numbers (4.9±0.8×$ 10^{8} $ cell $ l^{−1} $) in one system. Real-time PCR-amplified products obtained from genomic DNA extracted from samples were verified using clone library, and it revealed that only the target AOB DNA were PCR amplified, without amplification of the nontarget sequences. Activate Sludge (dpeaa)DE-He213 Clone Library (dpeaa)DE-He213 amoA Gene (dpeaa)DE-He213 Mixed Liquor Suspended Solid (dpeaa)DE-He213 Sewage Treatment System (dpeaa)DE-He213 Kurisu, Futoshi aut Yagi, Osami aut Enthalten in Applied microbiology and biotechnology Berlin : Springer, 1975 72(2006), 5 vom: 08. März, Seite 1004-1013 (DE-627)265509564 (DE-600)1464336-4 1432-0614 nnns volume:72 year:2006 number:5 day:08 month:03 pages:1004-1013 https://dx.doi.org/10.1007/s00253-006-0366-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2110 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 72 2006 5 08 03 1004-1013 |
| spelling |
10.1007/s00253-006-0366-x doi (DE-627)SPR002942909 (SPR)s00253-006-0366-x-e DE-627 ger DE-627 rakwb eng Limpiyakorn, Tawan verfasserin aut Development and application of real-time PCR for quantification of specific ammonia-oxidizing bacteria in activated sludge of sewage treatment systems 2006 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag 2006 Abstract In this study, four real-time polymerase chain reaction (PCR) primer sets were developed for the 16S rRNA genes of specific ammonia-oxidizing bacteria (AOB) found in activated sludge of sewage treatment systems. The primer sets target two of several sequence types of the Nitrosomonas oligotropha cluster, members within the Nitrosomonas communis cluster, and all members of the Nitrosomonas europaea–Nitrosococcus mobilis cluster. The detection limit of each primer set was in the range of 3×$ 10^{1} $–6×$ 10^{2} $ genes $ reaction^{−1} $. Reliable quantification of the target AOB DNA was obtained when the target AOB DNA comprised more than 0.1% of total AOB DNA in the sample. The application of the primer sets to samples taken from five sewage treatment systems showed that, in all systems, the majority of the AOB population was comprised of one sequence type of the N. oligotropha cluster (3.9±1.5×$ 10^{9} $–1.7±0.5×$ 10^{10} $ cell $ l^{−1} $) and, in most systems, followed by members within the N. communis cluster (2.8±0.3×$ 10^{9} $–1.0±0.1×$ 10^{10} $ cell $ l^{−1} $) or/and another sequence type of the N. oligotropha cluster (1.5±0.6×$ 10^{8} $–5.5±0.5×$ 10^{8} $ cell $ l^{−1} $). N. europaea–N. mobilis cluster arose solely in small numbers (4.9±0.8×$ 10^{8} $ cell $ l^{−1} $) in one system. Real-time PCR-amplified products obtained from genomic DNA extracted from samples were verified using clone library, and it revealed that only the target AOB DNA were PCR amplified, without amplification of the nontarget sequences. Activate Sludge (dpeaa)DE-He213 Clone Library (dpeaa)DE-He213 amoA Gene (dpeaa)DE-He213 Mixed Liquor Suspended Solid (dpeaa)DE-He213 Sewage Treatment System (dpeaa)DE-He213 Kurisu, Futoshi aut Yagi, Osami aut Enthalten in Applied microbiology and biotechnology Berlin : Springer, 1975 72(2006), 5 vom: 08. März, Seite 1004-1013 (DE-627)265509564 (DE-600)1464336-4 1432-0614 nnns volume:72 year:2006 number:5 day:08 month:03 pages:1004-1013 https://dx.doi.org/10.1007/s00253-006-0366-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2110 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 72 2006 5 08 03 1004-1013 |
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10.1007/s00253-006-0366-x doi (DE-627)SPR002942909 (SPR)s00253-006-0366-x-e DE-627 ger DE-627 rakwb eng Limpiyakorn, Tawan verfasserin aut Development and application of real-time PCR for quantification of specific ammonia-oxidizing bacteria in activated sludge of sewage treatment systems 2006 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag 2006 Abstract In this study, four real-time polymerase chain reaction (PCR) primer sets were developed for the 16S rRNA genes of specific ammonia-oxidizing bacteria (AOB) found in activated sludge of sewage treatment systems. The primer sets target two of several sequence types of the Nitrosomonas oligotropha cluster, members within the Nitrosomonas communis cluster, and all members of the Nitrosomonas europaea–Nitrosococcus mobilis cluster. The detection limit of each primer set was in the range of 3×$ 10^{1} $–6×$ 10^{2} $ genes $ reaction^{−1} $. Reliable quantification of the target AOB DNA was obtained when the target AOB DNA comprised more than 0.1% of total AOB DNA in the sample. The application of the primer sets to samples taken from five sewage treatment systems showed that, in all systems, the majority of the AOB population was comprised of one sequence type of the N. oligotropha cluster (3.9±1.5×$ 10^{9} $–1.7±0.5×$ 10^{10} $ cell $ l^{−1} $) and, in most systems, followed by members within the N. communis cluster (2.8±0.3×$ 10^{9} $–1.0±0.1×$ 10^{10} $ cell $ l^{−1} $) or/and another sequence type of the N. oligotropha cluster (1.5±0.6×$ 10^{8} $–5.5±0.5×$ 10^{8} $ cell $ l^{−1} $). N. europaea–N. mobilis cluster arose solely in small numbers (4.9±0.8×$ 10^{8} $ cell $ l^{−1} $) in one system. Real-time PCR-amplified products obtained from genomic DNA extracted from samples were verified using clone library, and it revealed that only the target AOB DNA were PCR amplified, without amplification of the nontarget sequences. Activate Sludge (dpeaa)DE-He213 Clone Library (dpeaa)DE-He213 amoA Gene (dpeaa)DE-He213 Mixed Liquor Suspended Solid (dpeaa)DE-He213 Sewage Treatment System (dpeaa)DE-He213 Kurisu, Futoshi aut Yagi, Osami aut Enthalten in Applied microbiology and biotechnology Berlin : Springer, 1975 72(2006), 5 vom: 08. März, Seite 1004-1013 (DE-627)265509564 (DE-600)1464336-4 1432-0614 nnns volume:72 year:2006 number:5 day:08 month:03 pages:1004-1013 https://dx.doi.org/10.1007/s00253-006-0366-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2110 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 72 2006 5 08 03 1004-1013 |
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10.1007/s00253-006-0366-x doi (DE-627)SPR002942909 (SPR)s00253-006-0366-x-e DE-627 ger DE-627 rakwb eng Limpiyakorn, Tawan verfasserin aut Development and application of real-time PCR for quantification of specific ammonia-oxidizing bacteria in activated sludge of sewage treatment systems 2006 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag 2006 Abstract In this study, four real-time polymerase chain reaction (PCR) primer sets were developed for the 16S rRNA genes of specific ammonia-oxidizing bacteria (AOB) found in activated sludge of sewage treatment systems. The primer sets target two of several sequence types of the Nitrosomonas oligotropha cluster, members within the Nitrosomonas communis cluster, and all members of the Nitrosomonas europaea–Nitrosococcus mobilis cluster. The detection limit of each primer set was in the range of 3×$ 10^{1} $–6×$ 10^{2} $ genes $ reaction^{−1} $. Reliable quantification of the target AOB DNA was obtained when the target AOB DNA comprised more than 0.1% of total AOB DNA in the sample. The application of the primer sets to samples taken from five sewage treatment systems showed that, in all systems, the majority of the AOB population was comprised of one sequence type of the N. oligotropha cluster (3.9±1.5×$ 10^{9} $–1.7±0.5×$ 10^{10} $ cell $ l^{−1} $) and, in most systems, followed by members within the N. communis cluster (2.8±0.3×$ 10^{9} $–1.0±0.1×$ 10^{10} $ cell $ l^{−1} $) or/and another sequence type of the N. oligotropha cluster (1.5±0.6×$ 10^{8} $–5.5±0.5×$ 10^{8} $ cell $ l^{−1} $). N. europaea–N. mobilis cluster arose solely in small numbers (4.9±0.8×$ 10^{8} $ cell $ l^{−1} $) in one system. Real-time PCR-amplified products obtained from genomic DNA extracted from samples were verified using clone library, and it revealed that only the target AOB DNA were PCR amplified, without amplification of the nontarget sequences. Activate Sludge (dpeaa)DE-He213 Clone Library (dpeaa)DE-He213 amoA Gene (dpeaa)DE-He213 Mixed Liquor Suspended Solid (dpeaa)DE-He213 Sewage Treatment System (dpeaa)DE-He213 Kurisu, Futoshi aut Yagi, Osami aut Enthalten in Applied microbiology and biotechnology Berlin : Springer, 1975 72(2006), 5 vom: 08. März, Seite 1004-1013 (DE-627)265509564 (DE-600)1464336-4 1432-0614 nnns volume:72 year:2006 number:5 day:08 month:03 pages:1004-1013 https://dx.doi.org/10.1007/s00253-006-0366-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2110 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 72 2006 5 08 03 1004-1013 |
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10.1007/s00253-006-0366-x doi (DE-627)SPR002942909 (SPR)s00253-006-0366-x-e DE-627 ger DE-627 rakwb eng Limpiyakorn, Tawan verfasserin aut Development and application of real-time PCR for quantification of specific ammonia-oxidizing bacteria in activated sludge of sewage treatment systems 2006 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag 2006 Abstract In this study, four real-time polymerase chain reaction (PCR) primer sets were developed for the 16S rRNA genes of specific ammonia-oxidizing bacteria (AOB) found in activated sludge of sewage treatment systems. The primer sets target two of several sequence types of the Nitrosomonas oligotropha cluster, members within the Nitrosomonas communis cluster, and all members of the Nitrosomonas europaea–Nitrosococcus mobilis cluster. The detection limit of each primer set was in the range of 3×$ 10^{1} $–6×$ 10^{2} $ genes $ reaction^{−1} $. Reliable quantification of the target AOB DNA was obtained when the target AOB DNA comprised more than 0.1% of total AOB DNA in the sample. The application of the primer sets to samples taken from five sewage treatment systems showed that, in all systems, the majority of the AOB population was comprised of one sequence type of the N. oligotropha cluster (3.9±1.5×$ 10^{9} $–1.7±0.5×$ 10^{10} $ cell $ l^{−1} $) and, in most systems, followed by members within the N. communis cluster (2.8±0.3×$ 10^{9} $–1.0±0.1×$ 10^{10} $ cell $ l^{−1} $) or/and another sequence type of the N. oligotropha cluster (1.5±0.6×$ 10^{8} $–5.5±0.5×$ 10^{8} $ cell $ l^{−1} $). N. europaea–N. mobilis cluster arose solely in small numbers (4.9±0.8×$ 10^{8} $ cell $ l^{−1} $) in one system. Real-time PCR-amplified products obtained from genomic DNA extracted from samples were verified using clone library, and it revealed that only the target AOB DNA were PCR amplified, without amplification of the nontarget sequences. Activate Sludge (dpeaa)DE-He213 Clone Library (dpeaa)DE-He213 amoA Gene (dpeaa)DE-He213 Mixed Liquor Suspended Solid (dpeaa)DE-He213 Sewage Treatment System (dpeaa)DE-He213 Kurisu, Futoshi aut Yagi, Osami aut Enthalten in Applied microbiology and biotechnology Berlin : Springer, 1975 72(2006), 5 vom: 08. März, Seite 1004-1013 (DE-627)265509564 (DE-600)1464336-4 1432-0614 nnns volume:72 year:2006 number:5 day:08 month:03 pages:1004-1013 https://dx.doi.org/10.1007/s00253-006-0366-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2110 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 72 2006 5 08 03 1004-1013 |
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English |
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Enthalten in Applied microbiology and biotechnology 72(2006), 5 vom: 08. März, Seite 1004-1013 volume:72 year:2006 number:5 day:08 month:03 pages:1004-1013 |
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Enthalten in Applied microbiology and biotechnology 72(2006), 5 vom: 08. März, Seite 1004-1013 volume:72 year:2006 number:5 day:08 month:03 pages:1004-1013 |
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Activate Sludge Clone Library amoA Gene Mixed Liquor Suspended Solid Sewage Treatment System |
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Limpiyakorn, Tawan @@aut@@ Kurisu, Futoshi @@aut@@ Yagi, Osami @@aut@@ |
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The primer sets target two of several sequence types of the Nitrosomonas oligotropha cluster, members within the Nitrosomonas communis cluster, and all members of the Nitrosomonas europaea–Nitrosococcus mobilis cluster. The detection limit of each primer set was in the range of 3×$ 10^{1} $–6×$ 10^{2} $ genes $ reaction^{−1} $. Reliable quantification of the target AOB DNA was obtained when the target AOB DNA comprised more than 0.1% of total AOB DNA in the sample. The application of the primer sets to samples taken from five sewage treatment systems showed that, in all systems, the majority of the AOB population was comprised of one sequence type of the N. oligotropha cluster (3.9±1.5×$ 10^{9} $–1.7±0.5×$ 10^{10} $ cell $ l^{−1} $) and, in most systems, followed by members within the N. communis cluster (2.8±0.3×$ 10^{9} $–1.0±0.1×$ 10^{10} $ cell $ l^{−1} $) or/and another sequence type of the N. oligotropha cluster (1.5±0.6×$ 10^{8} $–5.5±0.5×$ 10^{8} $ cell $ l^{−1} $). N. europaea–N. mobilis cluster arose solely in small numbers (4.9±0.8×$ 10^{8} $ cell $ l^{−1} $) in one system. 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Limpiyakorn, Tawan |
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Limpiyakorn, Tawan misc Activate Sludge misc Clone Library misc amoA Gene misc Mixed Liquor Suspended Solid misc Sewage Treatment System Development and application of real-time PCR for quantification of specific ammonia-oxidizing bacteria in activated sludge of sewage treatment systems |
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Development and application of real-time PCR for quantification of specific ammonia-oxidizing bacteria in activated sludge of sewage treatment systems Activate Sludge (dpeaa)DE-He213 Clone Library (dpeaa)DE-He213 amoA Gene (dpeaa)DE-He213 Mixed Liquor Suspended Solid (dpeaa)DE-He213 Sewage Treatment System (dpeaa)DE-He213 |
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Development and application of real-time PCR for quantification of specific ammonia-oxidizing bacteria in activated sludge of sewage treatment systems |
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Development and application of real-time PCR for quantification of specific ammonia-oxidizing bacteria in activated sludge of sewage treatment systems |
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Limpiyakorn, Tawan Kurisu, Futoshi Yagi, Osami |
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development and application of real-time pcr for quantification of specific ammonia-oxidizing bacteria in activated sludge of sewage treatment systems |
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Development and application of real-time PCR for quantification of specific ammonia-oxidizing bacteria in activated sludge of sewage treatment systems |
| abstract |
Abstract In this study, four real-time polymerase chain reaction (PCR) primer sets were developed for the 16S rRNA genes of specific ammonia-oxidizing bacteria (AOB) found in activated sludge of sewage treatment systems. The primer sets target two of several sequence types of the Nitrosomonas oligotropha cluster, members within the Nitrosomonas communis cluster, and all members of the Nitrosomonas europaea–Nitrosococcus mobilis cluster. The detection limit of each primer set was in the range of 3×$ 10^{1} $–6×$ 10^{2} $ genes $ reaction^{−1} $. Reliable quantification of the target AOB DNA was obtained when the target AOB DNA comprised more than 0.1% of total AOB DNA in the sample. The application of the primer sets to samples taken from five sewage treatment systems showed that, in all systems, the majority of the AOB population was comprised of one sequence type of the N. oligotropha cluster (3.9±1.5×$ 10^{9} $–1.7±0.5×$ 10^{10} $ cell $ l^{−1} $) and, in most systems, followed by members within the N. communis cluster (2.8±0.3×$ 10^{9} $–1.0±0.1×$ 10^{10} $ cell $ l^{−1} $) or/and another sequence type of the N. oligotropha cluster (1.5±0.6×$ 10^{8} $–5.5±0.5×$ 10^{8} $ cell $ l^{−1} $). N. europaea–N. mobilis cluster arose solely in small numbers (4.9±0.8×$ 10^{8} $ cell $ l^{−1} $) in one system. Real-time PCR-amplified products obtained from genomic DNA extracted from samples were verified using clone library, and it revealed that only the target AOB DNA were PCR amplified, without amplification of the nontarget sequences. © Springer-Verlag 2006 |
| abstractGer |
Abstract In this study, four real-time polymerase chain reaction (PCR) primer sets were developed for the 16S rRNA genes of specific ammonia-oxidizing bacteria (AOB) found in activated sludge of sewage treatment systems. The primer sets target two of several sequence types of the Nitrosomonas oligotropha cluster, members within the Nitrosomonas communis cluster, and all members of the Nitrosomonas europaea–Nitrosococcus mobilis cluster. The detection limit of each primer set was in the range of 3×$ 10^{1} $–6×$ 10^{2} $ genes $ reaction^{−1} $. Reliable quantification of the target AOB DNA was obtained when the target AOB DNA comprised more than 0.1% of total AOB DNA in the sample. The application of the primer sets to samples taken from five sewage treatment systems showed that, in all systems, the majority of the AOB population was comprised of one sequence type of the N. oligotropha cluster (3.9±1.5×$ 10^{9} $–1.7±0.5×$ 10^{10} $ cell $ l^{−1} $) and, in most systems, followed by members within the N. communis cluster (2.8±0.3×$ 10^{9} $–1.0±0.1×$ 10^{10} $ cell $ l^{−1} $) or/and another sequence type of the N. oligotropha cluster (1.5±0.6×$ 10^{8} $–5.5±0.5×$ 10^{8} $ cell $ l^{−1} $). N. europaea–N. mobilis cluster arose solely in small numbers (4.9±0.8×$ 10^{8} $ cell $ l^{−1} $) in one system. Real-time PCR-amplified products obtained from genomic DNA extracted from samples were verified using clone library, and it revealed that only the target AOB DNA were PCR amplified, without amplification of the nontarget sequences. © Springer-Verlag 2006 |
| abstract_unstemmed |
Abstract In this study, four real-time polymerase chain reaction (PCR) primer sets were developed for the 16S rRNA genes of specific ammonia-oxidizing bacteria (AOB) found in activated sludge of sewage treatment systems. The primer sets target two of several sequence types of the Nitrosomonas oligotropha cluster, members within the Nitrosomonas communis cluster, and all members of the Nitrosomonas europaea–Nitrosococcus mobilis cluster. The detection limit of each primer set was in the range of 3×$ 10^{1} $–6×$ 10^{2} $ genes $ reaction^{−1} $. Reliable quantification of the target AOB DNA was obtained when the target AOB DNA comprised more than 0.1% of total AOB DNA in the sample. The application of the primer sets to samples taken from five sewage treatment systems showed that, in all systems, the majority of the AOB population was comprised of one sequence type of the N. oligotropha cluster (3.9±1.5×$ 10^{9} $–1.7±0.5×$ 10^{10} $ cell $ l^{−1} $) and, in most systems, followed by members within the N. communis cluster (2.8±0.3×$ 10^{9} $–1.0±0.1×$ 10^{10} $ cell $ l^{−1} $) or/and another sequence type of the N. oligotropha cluster (1.5±0.6×$ 10^{8} $–5.5±0.5×$ 10^{8} $ cell $ l^{−1} $). N. europaea–N. mobilis cluster arose solely in small numbers (4.9±0.8×$ 10^{8} $ cell $ l^{−1} $) in one system. Real-time PCR-amplified products obtained from genomic DNA extracted from samples were verified using clone library, and it revealed that only the target AOB DNA were PCR amplified, without amplification of the nontarget sequences. © Springer-Verlag 2006 |
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| title_short |
Development and application of real-time PCR for quantification of specific ammonia-oxidizing bacteria in activated sludge of sewage treatment systems |
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https://dx.doi.org/10.1007/s00253-006-0366-x |
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Kurisu, Futoshi Yagi, Osami |
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2024-07-03T16:13:44.011Z |
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| score |
7.3992977 |