Improving intracellular production of recombinant protein in Pichia pastoris using an optimized preinduction glycerol-feeding scheme
Abstract High-cell-density production of recombinant growth hormone of Lateolabrax japonicus (rljGH) expressed intracellularly in Pichia pastoris was investigated. In the regular strategy of induction at a cell density of 160 g $ l^{−1} $, short duration of intracellular rljGH accumulation (17 h) re...
Ausführliche Beschreibung
Autor*in: |
Wei, Chun [verfasserIn] |
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Format: |
E-Artikel |
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Sprache: |
Englisch |
Erschienen: |
2008 |
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Schlagwörter: |
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Anmerkung: |
© Springer-Verlag 2007 |
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Übergeordnetes Werk: |
Enthalten in: Applied microbiology and biotechnology - Berlin : Springer, 1975, 78(2008), 2 vom: 01. Feb., Seite 257-264 |
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Übergeordnetes Werk: |
volume:78 ; year:2008 ; number:2 ; day:01 ; month:02 ; pages:257-264 |
Links: |
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DOI / URN: |
10.1007/s00253-007-1315-z |
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Katalog-ID: |
SPR002951924 |
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100 | 1 | |a Wei, Chun |e verfasserin |4 aut | |
245 | 1 | 0 | |a Improving intracellular production of recombinant protein in Pichia pastoris using an optimized preinduction glycerol-feeding scheme |
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520 | |a Abstract High-cell-density production of recombinant growth hormone of Lateolabrax japonicus (rljGH) expressed intracellularly in Pichia pastoris was investigated. In the regular strategy of induction at a cell density of 160 g $ l^{−1} $, short duration of intracellular rljGH accumulation (17 h) resulted in a low final cell density of 226 g $ l^{−1} $. Thus, a novel strategy of induction at a cell density of 320 g $ l^{−1} $ was investigated. In this strategy, the preinduction glycerol-feeding scheme had a significant effect on the post-induction production. Constant glycerol feeding led to a decrease of the specific rljGH production and specific production rate because of low preinduction specific growth rate. This decrease was avoided by exponential glycerol feeding to maintain a preinduction specific growth rate of 0.16 $ h^{−1} $. The results from exponential glycerol feeding indicated that the rljGH production depended on the preinduction specific growth rate. Moreover, mixed feeding of methanol and glycerol during induction improved the specific production rate to 0.07 mg $ g^{−1} $ $ h^{−1} $ from 0.043 mg $ g^{−1} $ $ h^{−1} $. Consequently, both high cell density (428 g $ l^{−1} $) and high rljGH production could be achieved by the novel strategy: growing the cells at the specific growth rate of 0.16 $ h^{−1} $ to the cell density of 320 g $ l^{−1} $ and inducing the expression by mixed feeding. | ||
650 | 4 | |a Exponential feeding |7 (dpeaa)DE-He213 | |
650 | 4 | |a High-cell-density fermentation |7 (dpeaa)DE-He213 | |
650 | 4 | |a Intracellular production |7 (dpeaa)DE-He213 | |
650 | 4 | |a Mixed feeding |7 (dpeaa)DE-He213 | |
650 | 4 | |a Specific growth rate |7 (dpeaa)DE-He213 | |
700 | 1 | |a Zhou, Xiangshan |4 aut | |
700 | 1 | |a Zhang, Yuanxing |4 aut | |
773 | 0 | 8 | |i Enthalten in |t Applied microbiology and biotechnology |d Berlin : Springer, 1975 |g 78(2008), 2 vom: 01. Feb., Seite 257-264 |w (DE-627)265509564 |w (DE-600)1464336-4 |x 1432-0614 |7 nnns |
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856 | 4 | 0 | |u https://dx.doi.org/10.1007/s00253-007-1315-z |z lizenzpflichtig |3 Volltext |
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912 | |a GBV_ILN_32 | ||
912 | |a GBV_ILN_39 | ||
912 | |a GBV_ILN_40 | ||
912 | |a GBV_ILN_60 | ||
912 | |a GBV_ILN_62 | ||
912 | |a GBV_ILN_63 | ||
912 | |a GBV_ILN_69 | ||
912 | |a GBV_ILN_70 | ||
912 | |a GBV_ILN_73 | ||
912 | |a GBV_ILN_74 | ||
912 | |a GBV_ILN_90 | ||
912 | |a GBV_ILN_95 | ||
912 | |a GBV_ILN_100 | ||
912 | |a GBV_ILN_101 | ||
912 | |a GBV_ILN_105 | ||
912 | |a GBV_ILN_110 | ||
912 | |a GBV_ILN_120 | ||
912 | |a GBV_ILN_138 | ||
912 | |a GBV_ILN_150 | ||
912 | |a GBV_ILN_151 | ||
912 | |a GBV_ILN_152 | ||
912 | |a GBV_ILN_161 | ||
912 | |a GBV_ILN_165 | ||
912 | |a GBV_ILN_170 | ||
912 | |a GBV_ILN_171 | ||
912 | |a GBV_ILN_187 | ||
912 | |a GBV_ILN_206 | ||
912 | |a GBV_ILN_213 | ||
912 | |a GBV_ILN_224 | ||
912 | |a GBV_ILN_230 | ||
912 | |a GBV_ILN_250 | ||
912 | |a GBV_ILN_267 | ||
912 | |a GBV_ILN_281 | ||
912 | |a GBV_ILN_285 | ||
912 | |a GBV_ILN_293 | ||
912 | |a GBV_ILN_370 | ||
912 | |a GBV_ILN_381 | ||
912 | |a GBV_ILN_602 | ||
912 | |a GBV_ILN_636 | ||
912 | |a GBV_ILN_702 | ||
912 | |a GBV_ILN_2001 | ||
912 | |a GBV_ILN_2003 | ||
912 | |a GBV_ILN_2004 | ||
912 | |a GBV_ILN_2005 | ||
912 | |a GBV_ILN_2006 | ||
912 | |a GBV_ILN_2007 | ||
912 | |a GBV_ILN_2008 | ||
912 | |a GBV_ILN_2009 | ||
912 | |a GBV_ILN_2010 | ||
912 | |a GBV_ILN_2011 | ||
912 | |a GBV_ILN_2014 | ||
912 | |a GBV_ILN_2015 | ||
912 | |a GBV_ILN_2020 | ||
912 | |a GBV_ILN_2021 | ||
912 | |a GBV_ILN_2025 | ||
912 | |a GBV_ILN_2026 | ||
912 | |a GBV_ILN_2027 | ||
912 | |a GBV_ILN_2031 | ||
912 | |a GBV_ILN_2034 | ||
912 | |a GBV_ILN_2037 | ||
912 | |a GBV_ILN_2038 | ||
912 | |a GBV_ILN_2039 | ||
912 | |a GBV_ILN_2044 | ||
912 | |a GBV_ILN_2048 | ||
912 | |a GBV_ILN_2049 | ||
912 | |a GBV_ILN_2050 | ||
912 | |a GBV_ILN_2055 | ||
912 | |a GBV_ILN_2056 | ||
912 | |a GBV_ILN_2057 | ||
912 | |a GBV_ILN_2059 | ||
912 | |a GBV_ILN_2061 | ||
912 | |a GBV_ILN_2064 | ||
912 | |a GBV_ILN_2068 | ||
912 | |a GBV_ILN_2070 | ||
912 | |a GBV_ILN_2086 | ||
912 | |a GBV_ILN_2093 | ||
912 | |a GBV_ILN_2106 | ||
912 | |a GBV_ILN_2107 | ||
912 | |a GBV_ILN_2110 | ||
912 | |a GBV_ILN_2113 | ||
912 | |a GBV_ILN_2118 | ||
912 | |a GBV_ILN_2119 | ||
912 | |a GBV_ILN_2129 | ||
912 | |a GBV_ILN_2143 | ||
912 | |a GBV_ILN_2144 | ||
912 | |a GBV_ILN_2147 | ||
912 | |a GBV_ILN_2153 | ||
912 | |a GBV_ILN_2188 | ||
912 | |a GBV_ILN_2232 | ||
912 | |a GBV_ILN_2336 | ||
912 | |a GBV_ILN_2446 | ||
912 | |a GBV_ILN_2470 | ||
912 | |a GBV_ILN_2472 | ||
912 | |a GBV_ILN_2507 | ||
912 | |a GBV_ILN_2522 | ||
912 | |a GBV_ILN_2548 | ||
912 | |a GBV_ILN_4012 | ||
912 | |a GBV_ILN_4035 | ||
912 | |a GBV_ILN_4037 | ||
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912 | |a GBV_ILN_4112 | ||
912 | |a GBV_ILN_4125 | ||
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912 | |a GBV_ILN_4246 | ||
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912 | |a GBV_ILN_4305 | ||
912 | |a GBV_ILN_4306 | ||
912 | |a GBV_ILN_4307 | ||
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912 | |a GBV_ILN_4323 | ||
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912 | |a GBV_ILN_4338 | ||
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10.1007/s00253-007-1315-z doi (DE-627)SPR002951924 (SPR)s00253-007-1315-z-e DE-627 ger DE-627 rakwb eng Wei, Chun verfasserin aut Improving intracellular production of recombinant protein in Pichia pastoris using an optimized preinduction glycerol-feeding scheme 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag 2007 Abstract High-cell-density production of recombinant growth hormone of Lateolabrax japonicus (rljGH) expressed intracellularly in Pichia pastoris was investigated. In the regular strategy of induction at a cell density of 160 g $ l^{−1} $, short duration of intracellular rljGH accumulation (17 h) resulted in a low final cell density of 226 g $ l^{−1} $. Thus, a novel strategy of induction at a cell density of 320 g $ l^{−1} $ was investigated. In this strategy, the preinduction glycerol-feeding scheme had a significant effect on the post-induction production. Constant glycerol feeding led to a decrease of the specific rljGH production and specific production rate because of low preinduction specific growth rate. This decrease was avoided by exponential glycerol feeding to maintain a preinduction specific growth rate of 0.16 $ h^{−1} $. The results from exponential glycerol feeding indicated that the rljGH production depended on the preinduction specific growth rate. Moreover, mixed feeding of methanol and glycerol during induction improved the specific production rate to 0.07 mg $ g^{−1} $ $ h^{−1} $ from 0.043 mg $ g^{−1} $ $ h^{−1} $. Consequently, both high cell density (428 g $ l^{−1} $) and high rljGH production could be achieved by the novel strategy: growing the cells at the specific growth rate of 0.16 $ h^{−1} $ to the cell density of 320 g $ l^{−1} $ and inducing the expression by mixed feeding. Exponential feeding (dpeaa)DE-He213 High-cell-density fermentation (dpeaa)DE-He213 Intracellular production (dpeaa)DE-He213 Mixed feeding (dpeaa)DE-He213 Specific growth rate (dpeaa)DE-He213 Zhou, Xiangshan aut Zhang, Yuanxing aut Enthalten in Applied microbiology and biotechnology Berlin : Springer, 1975 78(2008), 2 vom: 01. Feb., Seite 257-264 (DE-627)265509564 (DE-600)1464336-4 1432-0614 nnns volume:78 year:2008 number:2 day:01 month:02 pages:257-264 https://dx.doi.org/10.1007/s00253-007-1315-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2110 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 78 2008 2 01 02 257-264 |
spelling |
10.1007/s00253-007-1315-z doi (DE-627)SPR002951924 (SPR)s00253-007-1315-z-e DE-627 ger DE-627 rakwb eng Wei, Chun verfasserin aut Improving intracellular production of recombinant protein in Pichia pastoris using an optimized preinduction glycerol-feeding scheme 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag 2007 Abstract High-cell-density production of recombinant growth hormone of Lateolabrax japonicus (rljGH) expressed intracellularly in Pichia pastoris was investigated. In the regular strategy of induction at a cell density of 160 g $ l^{−1} $, short duration of intracellular rljGH accumulation (17 h) resulted in a low final cell density of 226 g $ l^{−1} $. Thus, a novel strategy of induction at a cell density of 320 g $ l^{−1} $ was investigated. In this strategy, the preinduction glycerol-feeding scheme had a significant effect on the post-induction production. Constant glycerol feeding led to a decrease of the specific rljGH production and specific production rate because of low preinduction specific growth rate. This decrease was avoided by exponential glycerol feeding to maintain a preinduction specific growth rate of 0.16 $ h^{−1} $. The results from exponential glycerol feeding indicated that the rljGH production depended on the preinduction specific growth rate. Moreover, mixed feeding of methanol and glycerol during induction improved the specific production rate to 0.07 mg $ g^{−1} $ $ h^{−1} $ from 0.043 mg $ g^{−1} $ $ h^{−1} $. Consequently, both high cell density (428 g $ l^{−1} $) and high rljGH production could be achieved by the novel strategy: growing the cells at the specific growth rate of 0.16 $ h^{−1} $ to the cell density of 320 g $ l^{−1} $ and inducing the expression by mixed feeding. Exponential feeding (dpeaa)DE-He213 High-cell-density fermentation (dpeaa)DE-He213 Intracellular production (dpeaa)DE-He213 Mixed feeding (dpeaa)DE-He213 Specific growth rate (dpeaa)DE-He213 Zhou, Xiangshan aut Zhang, Yuanxing aut Enthalten in Applied microbiology and biotechnology Berlin : Springer, 1975 78(2008), 2 vom: 01. Feb., Seite 257-264 (DE-627)265509564 (DE-600)1464336-4 1432-0614 nnns volume:78 year:2008 number:2 day:01 month:02 pages:257-264 https://dx.doi.org/10.1007/s00253-007-1315-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2110 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 78 2008 2 01 02 257-264 |
allfields_unstemmed |
10.1007/s00253-007-1315-z doi (DE-627)SPR002951924 (SPR)s00253-007-1315-z-e DE-627 ger DE-627 rakwb eng Wei, Chun verfasserin aut Improving intracellular production of recombinant protein in Pichia pastoris using an optimized preinduction glycerol-feeding scheme 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag 2007 Abstract High-cell-density production of recombinant growth hormone of Lateolabrax japonicus (rljGH) expressed intracellularly in Pichia pastoris was investigated. In the regular strategy of induction at a cell density of 160 g $ l^{−1} $, short duration of intracellular rljGH accumulation (17 h) resulted in a low final cell density of 226 g $ l^{−1} $. Thus, a novel strategy of induction at a cell density of 320 g $ l^{−1} $ was investigated. In this strategy, the preinduction glycerol-feeding scheme had a significant effect on the post-induction production. Constant glycerol feeding led to a decrease of the specific rljGH production and specific production rate because of low preinduction specific growth rate. This decrease was avoided by exponential glycerol feeding to maintain a preinduction specific growth rate of 0.16 $ h^{−1} $. The results from exponential glycerol feeding indicated that the rljGH production depended on the preinduction specific growth rate. Moreover, mixed feeding of methanol and glycerol during induction improved the specific production rate to 0.07 mg $ g^{−1} $ $ h^{−1} $ from 0.043 mg $ g^{−1} $ $ h^{−1} $. Consequently, both high cell density (428 g $ l^{−1} $) and high rljGH production could be achieved by the novel strategy: growing the cells at the specific growth rate of 0.16 $ h^{−1} $ to the cell density of 320 g $ l^{−1} $ and inducing the expression by mixed feeding. Exponential feeding (dpeaa)DE-He213 High-cell-density fermentation (dpeaa)DE-He213 Intracellular production (dpeaa)DE-He213 Mixed feeding (dpeaa)DE-He213 Specific growth rate (dpeaa)DE-He213 Zhou, Xiangshan aut Zhang, Yuanxing aut Enthalten in Applied microbiology and biotechnology Berlin : Springer, 1975 78(2008), 2 vom: 01. Feb., Seite 257-264 (DE-627)265509564 (DE-600)1464336-4 1432-0614 nnns volume:78 year:2008 number:2 day:01 month:02 pages:257-264 https://dx.doi.org/10.1007/s00253-007-1315-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2110 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 78 2008 2 01 02 257-264 |
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10.1007/s00253-007-1315-z doi (DE-627)SPR002951924 (SPR)s00253-007-1315-z-e DE-627 ger DE-627 rakwb eng Wei, Chun verfasserin aut Improving intracellular production of recombinant protein in Pichia pastoris using an optimized preinduction glycerol-feeding scheme 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag 2007 Abstract High-cell-density production of recombinant growth hormone of Lateolabrax japonicus (rljGH) expressed intracellularly in Pichia pastoris was investigated. In the regular strategy of induction at a cell density of 160 g $ l^{−1} $, short duration of intracellular rljGH accumulation (17 h) resulted in a low final cell density of 226 g $ l^{−1} $. Thus, a novel strategy of induction at a cell density of 320 g $ l^{−1} $ was investigated. In this strategy, the preinduction glycerol-feeding scheme had a significant effect on the post-induction production. Constant glycerol feeding led to a decrease of the specific rljGH production and specific production rate because of low preinduction specific growth rate. This decrease was avoided by exponential glycerol feeding to maintain a preinduction specific growth rate of 0.16 $ h^{−1} $. The results from exponential glycerol feeding indicated that the rljGH production depended on the preinduction specific growth rate. Moreover, mixed feeding of methanol and glycerol during induction improved the specific production rate to 0.07 mg $ g^{−1} $ $ h^{−1} $ from 0.043 mg $ g^{−1} $ $ h^{−1} $. Consequently, both high cell density (428 g $ l^{−1} $) and high rljGH production could be achieved by the novel strategy: growing the cells at the specific growth rate of 0.16 $ h^{−1} $ to the cell density of 320 g $ l^{−1} $ and inducing the expression by mixed feeding. Exponential feeding (dpeaa)DE-He213 High-cell-density fermentation (dpeaa)DE-He213 Intracellular production (dpeaa)DE-He213 Mixed feeding (dpeaa)DE-He213 Specific growth rate (dpeaa)DE-He213 Zhou, Xiangshan aut Zhang, Yuanxing aut Enthalten in Applied microbiology and biotechnology Berlin : Springer, 1975 78(2008), 2 vom: 01. Feb., Seite 257-264 (DE-627)265509564 (DE-600)1464336-4 1432-0614 nnns volume:78 year:2008 number:2 day:01 month:02 pages:257-264 https://dx.doi.org/10.1007/s00253-007-1315-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2110 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 78 2008 2 01 02 257-264 |
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10.1007/s00253-007-1315-z doi (DE-627)SPR002951924 (SPR)s00253-007-1315-z-e DE-627 ger DE-627 rakwb eng Wei, Chun verfasserin aut Improving intracellular production of recombinant protein in Pichia pastoris using an optimized preinduction glycerol-feeding scheme 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag 2007 Abstract High-cell-density production of recombinant growth hormone of Lateolabrax japonicus (rljGH) expressed intracellularly in Pichia pastoris was investigated. In the regular strategy of induction at a cell density of 160 g $ l^{−1} $, short duration of intracellular rljGH accumulation (17 h) resulted in a low final cell density of 226 g $ l^{−1} $. Thus, a novel strategy of induction at a cell density of 320 g $ l^{−1} $ was investigated. In this strategy, the preinduction glycerol-feeding scheme had a significant effect on the post-induction production. Constant glycerol feeding led to a decrease of the specific rljGH production and specific production rate because of low preinduction specific growth rate. This decrease was avoided by exponential glycerol feeding to maintain a preinduction specific growth rate of 0.16 $ h^{−1} $. The results from exponential glycerol feeding indicated that the rljGH production depended on the preinduction specific growth rate. Moreover, mixed feeding of methanol and glycerol during induction improved the specific production rate to 0.07 mg $ g^{−1} $ $ h^{−1} $ from 0.043 mg $ g^{−1} $ $ h^{−1} $. Consequently, both high cell density (428 g $ l^{−1} $) and high rljGH production could be achieved by the novel strategy: growing the cells at the specific growth rate of 0.16 $ h^{−1} $ to the cell density of 320 g $ l^{−1} $ and inducing the expression by mixed feeding. Exponential feeding (dpeaa)DE-He213 High-cell-density fermentation (dpeaa)DE-He213 Intracellular production (dpeaa)DE-He213 Mixed feeding (dpeaa)DE-He213 Specific growth rate (dpeaa)DE-He213 Zhou, Xiangshan aut Zhang, Yuanxing aut Enthalten in Applied microbiology and biotechnology Berlin : Springer, 1975 78(2008), 2 vom: 01. Feb., Seite 257-264 (DE-627)265509564 (DE-600)1464336-4 1432-0614 nnns volume:78 year:2008 number:2 day:01 month:02 pages:257-264 https://dx.doi.org/10.1007/s00253-007-1315-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2110 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 78 2008 2 01 02 257-264 |
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Enthalten in Applied microbiology and biotechnology 78(2008), 2 vom: 01. Feb., Seite 257-264 volume:78 year:2008 number:2 day:01 month:02 pages:257-264 |
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Enthalten in Applied microbiology and biotechnology 78(2008), 2 vom: 01. Feb., Seite 257-264 volume:78 year:2008 number:2 day:01 month:02 pages:257-264 |
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Applied microbiology and biotechnology |
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Wei, Chun @@aut@@ Zhou, Xiangshan @@aut@@ Zhang, Yuanxing @@aut@@ |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">SPR002951924</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230519194128.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">201001s2008 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/s00253-007-1315-z</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)SPR002951924</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(SPR)s00253-007-1315-z-e</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Wei, Chun</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Improving intracellular production of recombinant protein in Pichia pastoris using an optimized preinduction glycerol-feeding scheme</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2008</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">© Springer-Verlag 2007</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract High-cell-density production of recombinant growth hormone of Lateolabrax japonicus (rljGH) expressed intracellularly in Pichia pastoris was investigated. In the regular strategy of induction at a cell density of 160 g $ l^{−1} $, short duration of intracellular rljGH accumulation (17 h) resulted in a low final cell density of 226 g $ l^{−1} $. Thus, a novel strategy of induction at a cell density of 320 g $ l^{−1} $ was investigated. In this strategy, the preinduction glycerol-feeding scheme had a significant effect on the post-induction production. Constant glycerol feeding led to a decrease of the specific rljGH production and specific production rate because of low preinduction specific growth rate. This decrease was avoided by exponential glycerol feeding to maintain a preinduction specific growth rate of 0.16 $ h^{−1} $. The results from exponential glycerol feeding indicated that the rljGH production depended on the preinduction specific growth rate. Moreover, mixed feeding of methanol and glycerol during induction improved the specific production rate to 0.07 mg $ g^{−1} $ $ h^{−1} $ from 0.043 mg $ g^{−1} $ $ h^{−1} $. 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Wei, Chun misc Exponential feeding misc High-cell-density fermentation misc Intracellular production misc Mixed feeding misc Specific growth rate Improving intracellular production of recombinant protein in Pichia pastoris using an optimized preinduction glycerol-feeding scheme |
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Improving intracellular production of recombinant protein in Pichia pastoris using an optimized preinduction glycerol-feeding scheme Exponential feeding (dpeaa)DE-He213 High-cell-density fermentation (dpeaa)DE-He213 Intracellular production (dpeaa)DE-He213 Mixed feeding (dpeaa)DE-He213 Specific growth rate (dpeaa)DE-He213 |
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improving intracellular production of recombinant protein in pichia pastoris using an optimized preinduction glycerol-feeding scheme |
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Improving intracellular production of recombinant protein in Pichia pastoris using an optimized preinduction glycerol-feeding scheme |
abstract |
Abstract High-cell-density production of recombinant growth hormone of Lateolabrax japonicus (rljGH) expressed intracellularly in Pichia pastoris was investigated. In the regular strategy of induction at a cell density of 160 g $ l^{−1} $, short duration of intracellular rljGH accumulation (17 h) resulted in a low final cell density of 226 g $ l^{−1} $. Thus, a novel strategy of induction at a cell density of 320 g $ l^{−1} $ was investigated. In this strategy, the preinduction glycerol-feeding scheme had a significant effect on the post-induction production. Constant glycerol feeding led to a decrease of the specific rljGH production and specific production rate because of low preinduction specific growth rate. This decrease was avoided by exponential glycerol feeding to maintain a preinduction specific growth rate of 0.16 $ h^{−1} $. The results from exponential glycerol feeding indicated that the rljGH production depended on the preinduction specific growth rate. Moreover, mixed feeding of methanol and glycerol during induction improved the specific production rate to 0.07 mg $ g^{−1} $ $ h^{−1} $ from 0.043 mg $ g^{−1} $ $ h^{−1} $. Consequently, both high cell density (428 g $ l^{−1} $) and high rljGH production could be achieved by the novel strategy: growing the cells at the specific growth rate of 0.16 $ h^{−1} $ to the cell density of 320 g $ l^{−1} $ and inducing the expression by mixed feeding. © Springer-Verlag 2007 |
abstractGer |
Abstract High-cell-density production of recombinant growth hormone of Lateolabrax japonicus (rljGH) expressed intracellularly in Pichia pastoris was investigated. In the regular strategy of induction at a cell density of 160 g $ l^{−1} $, short duration of intracellular rljGH accumulation (17 h) resulted in a low final cell density of 226 g $ l^{−1} $. Thus, a novel strategy of induction at a cell density of 320 g $ l^{−1} $ was investigated. In this strategy, the preinduction glycerol-feeding scheme had a significant effect on the post-induction production. Constant glycerol feeding led to a decrease of the specific rljGH production and specific production rate because of low preinduction specific growth rate. This decrease was avoided by exponential glycerol feeding to maintain a preinduction specific growth rate of 0.16 $ h^{−1} $. The results from exponential glycerol feeding indicated that the rljGH production depended on the preinduction specific growth rate. Moreover, mixed feeding of methanol and glycerol during induction improved the specific production rate to 0.07 mg $ g^{−1} $ $ h^{−1} $ from 0.043 mg $ g^{−1} $ $ h^{−1} $. Consequently, both high cell density (428 g $ l^{−1} $) and high rljGH production could be achieved by the novel strategy: growing the cells at the specific growth rate of 0.16 $ h^{−1} $ to the cell density of 320 g $ l^{−1} $ and inducing the expression by mixed feeding. © Springer-Verlag 2007 |
abstract_unstemmed |
Abstract High-cell-density production of recombinant growth hormone of Lateolabrax japonicus (rljGH) expressed intracellularly in Pichia pastoris was investigated. In the regular strategy of induction at a cell density of 160 g $ l^{−1} $, short duration of intracellular rljGH accumulation (17 h) resulted in a low final cell density of 226 g $ l^{−1} $. Thus, a novel strategy of induction at a cell density of 320 g $ l^{−1} $ was investigated. In this strategy, the preinduction glycerol-feeding scheme had a significant effect on the post-induction production. Constant glycerol feeding led to a decrease of the specific rljGH production and specific production rate because of low preinduction specific growth rate. This decrease was avoided by exponential glycerol feeding to maintain a preinduction specific growth rate of 0.16 $ h^{−1} $. The results from exponential glycerol feeding indicated that the rljGH production depended on the preinduction specific growth rate. Moreover, mixed feeding of methanol and glycerol during induction improved the specific production rate to 0.07 mg $ g^{−1} $ $ h^{−1} $ from 0.043 mg $ g^{−1} $ $ h^{−1} $. Consequently, both high cell density (428 g $ l^{−1} $) and high rljGH production could be achieved by the novel strategy: growing the cells at the specific growth rate of 0.16 $ h^{−1} $ to the cell density of 320 g $ l^{−1} $ and inducing the expression by mixed feeding. © Springer-Verlag 2007 |
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container_issue |
2 |
title_short |
Improving intracellular production of recombinant protein in Pichia pastoris using an optimized preinduction glycerol-feeding scheme |
url |
https://dx.doi.org/10.1007/s00253-007-1315-z |
remote_bool |
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author2 |
Zhou, Xiangshan Zhang, Yuanxing |
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Zhou, Xiangshan Zhang, Yuanxing |
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doi_str |
10.1007/s00253-007-1315-z |
up_date |
2024-07-03T16:17:26.121Z |
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score |
7.400736 |