Lactic fermentation of cellobiose by a yeast strain displaying β-glucosidase on the cell surface

Abstract The Aspergillus aculeatus β-glucosidase 1 (bgl1) gene was expressed in a lactic-acid-producing Saccharomyces cerevisiae strain to enable lactic fermentation with cellobiose. The recombinant β-glucosidase enzyme was expressed on the yeast cell surface by fusing the mature protein to the C-te...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Tokuhiro, Kenro [verfasserIn] Ishida, Nobuhiro Kondo, Akihiko Takahashi, Haruo

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2008

Schlagwörter:	Yeast Beta-glucosidase -Lactate Cellobiose Cellulase

Anmerkung:	© Springer-Verlag 2008

Übergeordnetes Werk:	Enthalten in: Applied microbiology and biotechnology - Berlin : Springer, 1975, 79(2008), 3 vom: 01. Juni
Übergeordnetes Werk:	volume:79 ; year:2008 ; number:3 ; day:01 ; month:06

Links:	Volltext

DOI / URN:	10.1007/s00253-008-1454-x

Katalog-ID:	SPR002953315

Internformat


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100	1		\|a Tokuhiro, Kenro \|e verfasserin \|4 aut
245	1	0	\|a Lactic fermentation of cellobiose by a yeast strain displaying β-glucosidase on the cell surface
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520			\|a Abstract The Aspergillus aculeatus β-glucosidase 1 (bgl1) gene was expressed in a lactic-acid-producing Saccharomyces cerevisiae strain to enable lactic fermentation with cellobiose. The recombinant β-glucosidase enzyme was expressed on the yeast cell surface by fusing the mature protein to the C-terminal half region of the α-agglutinin. The β-glucosidase expression plasmids were integrated into the genome. Three strong promoters of S. cerevisiae, the TDH3, PGK1, and PDC1 promoters, were used for β-glucosidase expression. The specific β-glucosidase activity varied with the promoter used and the copy number of the bgl1 gene. The highest activity was obtained with strain PB2 that possessed two copies of the bgl1 gene driven by the PDC1 promoter. PB2 could grow on cellobiose and glucose minimal medium at the same rate. Fermentation experiments were conducted in non-selective-rich media containing 95 g $ l^{−1} $ cellobiose or 100 g $ l^{−1} $ glucose as a carbon source under microaerobic conditions. The maximum rate of l-lactate production by PB2 on cellobiose (2.8 g $ l^{−1} $ $ h^{−1} $) was similar to that on glucose (3.0 g $ l^{−1} $ $ h^{−1} $). This indicates that efficient fermentation of cellobiose to l-lactate can be accomplished using a yeast strain expressing β-glucosidase from a mitotically stable genomic integration plasmid.
650		4	\|a Yeast \|7 (dpeaa)DE-He213
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650		4	\|a Cellulase \|7 (dpeaa)DE-He213
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700	1		\|a Kondo, Akihiko \|4 aut
700	1		\|a Takahashi, Haruo \|4 aut
773	0	8	\|i Enthalten in \|t Applied microbiology and biotechnology \|d Berlin : Springer, 1975 \|g 79(2008), 3 vom: 01. Juni \|w (DE-627)265509564 \|w (DE-600)1464336-4 \|x 1432-0614 \|7 nnns
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912			\|a GBV_USEFLAG_A
912			\|a SYSFLAG_A
912			\|a GBV_SPRINGER
912			\|a SSG-OLC-PHA
912			\|a GBV_ILN_11
912			\|a GBV_ILN_20
912			\|a GBV_ILN_22
912			\|a GBV_ILN_23
912			\|a GBV_ILN_24
912			\|a GBV_ILN_31
912			\|a GBV_ILN_32
912			\|a GBV_ILN_39
912			\|a GBV_ILN_40
912			\|a GBV_ILN_60
912			\|a GBV_ILN_62
912			\|a GBV_ILN_63
912			\|a GBV_ILN_69
912			\|a GBV_ILN_70
912			\|a GBV_ILN_73
912			\|a GBV_ILN_74
912			\|a GBV_ILN_90
912			\|a GBV_ILN_95
912			\|a GBV_ILN_100
912			\|a GBV_ILN_101
912			\|a GBV_ILN_105
912			\|a GBV_ILN_110
912			\|a GBV_ILN_120
912			\|a GBV_ILN_138
912			\|a GBV_ILN_150
912			\|a GBV_ILN_151
912			\|a GBV_ILN_152
912			\|a GBV_ILN_161
912			\|a GBV_ILN_165
912			\|a GBV_ILN_170
912			\|a GBV_ILN_171
912			\|a GBV_ILN_187
912			\|a GBV_ILN_206
912			\|a GBV_ILN_213
912			\|a GBV_ILN_224
912			\|a GBV_ILN_230
912			\|a GBV_ILN_250
912			\|a GBV_ILN_267
912			\|a GBV_ILN_281
912			\|a GBV_ILN_285
912			\|a GBV_ILN_293
912			\|a GBV_ILN_370
912			\|a GBV_ILN_381
912			\|a GBV_ILN_602
912			\|a GBV_ILN_636
912			\|a GBV_ILN_702
912			\|a GBV_ILN_2001
912			\|a GBV_ILN_2003
912			\|a GBV_ILN_2004
912			\|a GBV_ILN_2005
912			\|a GBV_ILN_2006
912			\|a GBV_ILN_2007
912			\|a GBV_ILN_2008
912			\|a GBV_ILN_2009
912			\|a GBV_ILN_2010
912			\|a GBV_ILN_2011
912			\|a GBV_ILN_2014
912			\|a GBV_ILN_2015
912			\|a GBV_ILN_2020
912			\|a GBV_ILN_2021
912			\|a GBV_ILN_2025
912			\|a GBV_ILN_2026
912			\|a GBV_ILN_2027
912			\|a GBV_ILN_2031
912			\|a GBV_ILN_2034
912			\|a GBV_ILN_2037
912			\|a GBV_ILN_2038
912			\|a GBV_ILN_2039
912			\|a GBV_ILN_2044
912			\|a GBV_ILN_2048
912			\|a GBV_ILN_2049
912			\|a GBV_ILN_2050
912			\|a GBV_ILN_2055
912			\|a GBV_ILN_2056
912			\|a GBV_ILN_2057
912			\|a GBV_ILN_2059
912			\|a GBV_ILN_2061
912			\|a GBV_ILN_2064
912			\|a GBV_ILN_2068
912			\|a GBV_ILN_2070
912			\|a GBV_ILN_2086
912			\|a GBV_ILN_2093
912			\|a GBV_ILN_2106
912			\|a GBV_ILN_2107
912			\|a GBV_ILN_2110
912			\|a GBV_ILN_2113
912			\|a GBV_ILN_2118
912			\|a GBV_ILN_2119
912			\|a GBV_ILN_2129
912			\|a GBV_ILN_2143
912			\|a GBV_ILN_2144
912			\|a GBV_ILN_2147
912			\|a GBV_ILN_2153
912			\|a GBV_ILN_2188
912			\|a GBV_ILN_2232
912			\|a GBV_ILN_2336
912			\|a GBV_ILN_2446
912			\|a GBV_ILN_2470
912			\|a GBV_ILN_2472
912			\|a GBV_ILN_2507
912			\|a GBV_ILN_2522
912			\|a GBV_ILN_2548
912			\|a GBV_ILN_4012
912			\|a GBV_ILN_4035
912			\|a GBV_ILN_4037
912			\|a GBV_ILN_4046
912			\|a GBV_ILN_4112
912			\|a GBV_ILN_4125
912			\|a GBV_ILN_4126
912			\|a GBV_ILN_4242
912			\|a GBV_ILN_4246
912			\|a GBV_ILN_4249
912			\|a GBV_ILN_4251
912			\|a GBV_ILN_4305
912			\|a GBV_ILN_4306
912			\|a GBV_ILN_4307
912			\|a GBV_ILN_4313
912			\|a GBV_ILN_4322
912			\|a GBV_ILN_4323
912			\|a GBV_ILN_4324
912			\|a GBV_ILN_4326
912			\|a GBV_ILN_4333
912			\|a GBV_ILN_4334
912			\|a GBV_ILN_4335
912			\|a GBV_ILN_4336
912			\|a GBV_ILN_4338
912			\|a GBV_ILN_4393
912			\|a GBV_ILN_4700
951			\|a AR
952			\|d 79 \|j 2008 \|e 3 \|b 01 \|c 06

Indexfelder

author_variant	k t kt n i ni a k ak h t ht
matchkey_str	article:14320614:2008----::atcemnainfelboeyyattanipaiglcs
hierarchy_sort_str	2008
publishDate	2008
allfields	10.1007/s00253-008-1454-x doi (DE-627)SPR002953315 (SPR)s00253-008-1454-x-e DE-627 ger DE-627 rakwb eng Tokuhiro, Kenro verfasserin aut Lactic fermentation of cellobiose by a yeast strain displaying β-glucosidase on the cell surface 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag 2008 Abstract The Aspergillus aculeatus β-glucosidase 1 (bgl1) gene was expressed in a lactic-acid-producing Saccharomyces cerevisiae strain to enable lactic fermentation with cellobiose. The recombinant β-glucosidase enzyme was expressed on the yeast cell surface by fusing the mature protein to the C-terminal half region of the α-agglutinin. The β-glucosidase expression plasmids were integrated into the genome. Three strong promoters of S. cerevisiae, the TDH3, PGK1, and PDC1 promoters, were used for β-glucosidase expression. The specific β-glucosidase activity varied with the promoter used and the copy number of the bgl1 gene. The highest activity was obtained with strain PB2 that possessed two copies of the bgl1 gene driven by the PDC1 promoter. PB2 could grow on cellobiose and glucose minimal medium at the same rate. Fermentation experiments were conducted in non-selective-rich media containing 95 g $ l^{−1} $ cellobiose or 100 g $ l^{−1} $ glucose as a carbon source under microaerobic conditions. The maximum rate of l-lactate production by PB2 on cellobiose (2.8 g $ l^{−1} $ $ h^{−1} $) was similar to that on glucose (3.0 g $ l^{−1} $ $ h^{−1} $). This indicates that efficient fermentation of cellobiose to l-lactate can be accomplished using a yeast strain expressing β-glucosidase from a mitotically stable genomic integration plasmid. Yeast (dpeaa)DE-He213 Beta-glucosidase (dpeaa)DE-He213 -Lactate (dpeaa)DE-He213 Cellobiose (dpeaa)DE-He213 Cellulase (dpeaa)DE-He213 Ishida, Nobuhiro aut Kondo, Akihiko aut Takahashi, Haruo aut Enthalten in Applied microbiology and biotechnology Berlin : Springer, 1975 79(2008), 3 vom: 01. Juni (DE-627)265509564 (DE-600)1464336-4 1432-0614 nnns volume:79 year:2008 number:3 day:01 month:06 https://dx.doi.org/10.1007/s00253-008-1454-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2110 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 79 2008 3 01 06
spelling	10.1007/s00253-008-1454-x doi (DE-627)SPR002953315 (SPR)s00253-008-1454-x-e DE-627 ger DE-627 rakwb eng Tokuhiro, Kenro verfasserin aut Lactic fermentation of cellobiose by a yeast strain displaying β-glucosidase on the cell surface 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag 2008 Abstract The Aspergillus aculeatus β-glucosidase 1 (bgl1) gene was expressed in a lactic-acid-producing Saccharomyces cerevisiae strain to enable lactic fermentation with cellobiose. The recombinant β-glucosidase enzyme was expressed on the yeast cell surface by fusing the mature protein to the C-terminal half region of the α-agglutinin. The β-glucosidase expression plasmids were integrated into the genome. Three strong promoters of S. cerevisiae, the TDH3, PGK1, and PDC1 promoters, were used for β-glucosidase expression. The specific β-glucosidase activity varied with the promoter used and the copy number of the bgl1 gene. The highest activity was obtained with strain PB2 that possessed two copies of the bgl1 gene driven by the PDC1 promoter. PB2 could grow on cellobiose and glucose minimal medium at the same rate. Fermentation experiments were conducted in non-selective-rich media containing 95 g $ l^{−1} $ cellobiose or 100 g $ l^{−1} $ glucose as a carbon source under microaerobic conditions. The maximum rate of l-lactate production by PB2 on cellobiose (2.8 g $ l^{−1} $ $ h^{−1} $) was similar to that on glucose (3.0 g $ l^{−1} $ $ h^{−1} $). This indicates that efficient fermentation of cellobiose to l-lactate can be accomplished using a yeast strain expressing β-glucosidase from a mitotically stable genomic integration plasmid. Yeast (dpeaa)DE-He213 Beta-glucosidase (dpeaa)DE-He213 -Lactate (dpeaa)DE-He213 Cellobiose (dpeaa)DE-He213 Cellulase (dpeaa)DE-He213 Ishida, Nobuhiro aut Kondo, Akihiko aut Takahashi, Haruo aut Enthalten in Applied microbiology and biotechnology Berlin : Springer, 1975 79(2008), 3 vom: 01. Juni (DE-627)265509564 (DE-600)1464336-4 1432-0614 nnns volume:79 year:2008 number:3 day:01 month:06 https://dx.doi.org/10.1007/s00253-008-1454-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2110 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 79 2008 3 01 06
allfields_unstemmed	10.1007/s00253-008-1454-x doi (DE-627)SPR002953315 (SPR)s00253-008-1454-x-e DE-627 ger DE-627 rakwb eng Tokuhiro, Kenro verfasserin aut Lactic fermentation of cellobiose by a yeast strain displaying β-glucosidase on the cell surface 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag 2008 Abstract The Aspergillus aculeatus β-glucosidase 1 (bgl1) gene was expressed in a lactic-acid-producing Saccharomyces cerevisiae strain to enable lactic fermentation with cellobiose. The recombinant β-glucosidase enzyme was expressed on the yeast cell surface by fusing the mature protein to the C-terminal half region of the α-agglutinin. The β-glucosidase expression plasmids were integrated into the genome. Three strong promoters of S. cerevisiae, the TDH3, PGK1, and PDC1 promoters, were used for β-glucosidase expression. The specific β-glucosidase activity varied with the promoter used and the copy number of the bgl1 gene. The highest activity was obtained with strain PB2 that possessed two copies of the bgl1 gene driven by the PDC1 promoter. PB2 could grow on cellobiose and glucose minimal medium at the same rate. Fermentation experiments were conducted in non-selective-rich media containing 95 g $ l^{−1} $ cellobiose or 100 g $ l^{−1} $ glucose as a carbon source under microaerobic conditions. The maximum rate of l-lactate production by PB2 on cellobiose (2.8 g $ l^{−1} $ $ h^{−1} $) was similar to that on glucose (3.0 g $ l^{−1} $ $ h^{−1} $). This indicates that efficient fermentation of cellobiose to l-lactate can be accomplished using a yeast strain expressing β-glucosidase from a mitotically stable genomic integration plasmid. Yeast (dpeaa)DE-He213 Beta-glucosidase (dpeaa)DE-He213 -Lactate (dpeaa)DE-He213 Cellobiose (dpeaa)DE-He213 Cellulase (dpeaa)DE-He213 Ishida, Nobuhiro aut Kondo, Akihiko aut Takahashi, Haruo aut Enthalten in Applied microbiology and biotechnology Berlin : Springer, 1975 79(2008), 3 vom: 01. Juni (DE-627)265509564 (DE-600)1464336-4 1432-0614 nnns volume:79 year:2008 number:3 day:01 month:06 https://dx.doi.org/10.1007/s00253-008-1454-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2110 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 79 2008 3 01 06
allfieldsGer	10.1007/s00253-008-1454-x doi (DE-627)SPR002953315 (SPR)s00253-008-1454-x-e DE-627 ger DE-627 rakwb eng Tokuhiro, Kenro verfasserin aut Lactic fermentation of cellobiose by a yeast strain displaying β-glucosidase on the cell surface 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag 2008 Abstract The Aspergillus aculeatus β-glucosidase 1 (bgl1) gene was expressed in a lactic-acid-producing Saccharomyces cerevisiae strain to enable lactic fermentation with cellobiose. The recombinant β-glucosidase enzyme was expressed on the yeast cell surface by fusing the mature protein to the C-terminal half region of the α-agglutinin. The β-glucosidase expression plasmids were integrated into the genome. Three strong promoters of S. cerevisiae, the TDH3, PGK1, and PDC1 promoters, were used for β-glucosidase expression. The specific β-glucosidase activity varied with the promoter used and the copy number of the bgl1 gene. The highest activity was obtained with strain PB2 that possessed two copies of the bgl1 gene driven by the PDC1 promoter. PB2 could grow on cellobiose and glucose minimal medium at the same rate. Fermentation experiments were conducted in non-selective-rich media containing 95 g $ l^{−1} $ cellobiose or 100 g $ l^{−1} $ glucose as a carbon source under microaerobic conditions. The maximum rate of l-lactate production by PB2 on cellobiose (2.8 g $ l^{−1} $ $ h^{−1} $) was similar to that on glucose (3.0 g $ l^{−1} $ $ h^{−1} $). This indicates that efficient fermentation of cellobiose to l-lactate can be accomplished using a yeast strain expressing β-glucosidase from a mitotically stable genomic integration plasmid. Yeast (dpeaa)DE-He213 Beta-glucosidase (dpeaa)DE-He213 -Lactate (dpeaa)DE-He213 Cellobiose (dpeaa)DE-He213 Cellulase (dpeaa)DE-He213 Ishida, Nobuhiro aut Kondo, Akihiko aut Takahashi, Haruo aut Enthalten in Applied microbiology and biotechnology Berlin : Springer, 1975 79(2008), 3 vom: 01. Juni (DE-627)265509564 (DE-600)1464336-4 1432-0614 nnns volume:79 year:2008 number:3 day:01 month:06 https://dx.doi.org/10.1007/s00253-008-1454-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2110 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 79 2008 3 01 06
allfieldsSound	10.1007/s00253-008-1454-x doi (DE-627)SPR002953315 (SPR)s00253-008-1454-x-e DE-627 ger DE-627 rakwb eng Tokuhiro, Kenro verfasserin aut Lactic fermentation of cellobiose by a yeast strain displaying β-glucosidase on the cell surface 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag 2008 Abstract The Aspergillus aculeatus β-glucosidase 1 (bgl1) gene was expressed in a lactic-acid-producing Saccharomyces cerevisiae strain to enable lactic fermentation with cellobiose. The recombinant β-glucosidase enzyme was expressed on the yeast cell surface by fusing the mature protein to the C-terminal half region of the α-agglutinin. The β-glucosidase expression plasmids were integrated into the genome. Three strong promoters of S. cerevisiae, the TDH3, PGK1, and PDC1 promoters, were used for β-glucosidase expression. The specific β-glucosidase activity varied with the promoter used and the copy number of the bgl1 gene. The highest activity was obtained with strain PB2 that possessed two copies of the bgl1 gene driven by the PDC1 promoter. PB2 could grow on cellobiose and glucose minimal medium at the same rate. Fermentation experiments were conducted in non-selective-rich media containing 95 g $ l^{−1} $ cellobiose or 100 g $ l^{−1} $ glucose as a carbon source under microaerobic conditions. The maximum rate of l-lactate production by PB2 on cellobiose (2.8 g $ l^{−1} $ $ h^{−1} $) was similar to that on glucose (3.0 g $ l^{−1} $ $ h^{−1} $). This indicates that efficient fermentation of cellobiose to l-lactate can be accomplished using a yeast strain expressing β-glucosidase from a mitotically stable genomic integration plasmid. Yeast (dpeaa)DE-He213 Beta-glucosidase (dpeaa)DE-He213 -Lactate (dpeaa)DE-He213 Cellobiose (dpeaa)DE-He213 Cellulase (dpeaa)DE-He213 Ishida, Nobuhiro aut Kondo, Akihiko aut Takahashi, Haruo aut Enthalten in Applied microbiology and biotechnology Berlin : Springer, 1975 79(2008), 3 vom: 01. Juni (DE-627)265509564 (DE-600)1464336-4 1432-0614 nnns volume:79 year:2008 number:3 day:01 month:06 https://dx.doi.org/10.1007/s00253-008-1454-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2110 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 79 2008 3 01 06
language	English
source	Enthalten in Applied microbiology and biotechnology 79(2008), 3 vom: 01. Juni volume:79 year:2008 number:3 day:01 month:06
sourceStr	Enthalten in Applied microbiology and biotechnology 79(2008), 3 vom: 01. Juni volume:79 year:2008 number:3 day:01 month:06
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Yeast Beta-glucosidase -Lactate Cellobiose Cellulase
isfreeaccess_bool	false
container_title	Applied microbiology and biotechnology
authorswithroles_txt_mv	Tokuhiro, Kenro @@aut@@ Ishida, Nobuhiro @@aut@@ Kondo, Akihiko @@aut@@ Takahashi, Haruo @@aut@@
publishDateDaySort_date	2008-06-01T00:00:00Z
hierarchy_top_id	265509564
id	SPR002953315
language_de	englisch
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author	Tokuhiro, Kenro
spellingShingle	Tokuhiro, Kenro misc Yeast misc Beta-glucosidase misc -Lactate misc Cellobiose misc Cellulase Lactic fermentation of cellobiose by a yeast strain displaying β-glucosidase on the cell surface
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topic_title	Lactic fermentation of cellobiose by a yeast strain displaying β-glucosidase on the cell surface Yeast (dpeaa)DE-He213 Beta-glucosidase (dpeaa)DE-He213 -Lactate (dpeaa)DE-He213 Cellobiose (dpeaa)DE-He213 Cellulase (dpeaa)DE-He213
topic	misc Yeast misc Beta-glucosidase misc -Lactate misc Cellobiose misc Cellulase
topic_unstemmed	misc Yeast misc Beta-glucosidase misc -Lactate misc Cellobiose misc Cellulase
topic_browse	misc Yeast misc Beta-glucosidase misc -Lactate misc Cellobiose misc Cellulase
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title	Lactic fermentation of cellobiose by a yeast strain displaying β-glucosidase on the cell surface
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title_full	Lactic fermentation of cellobiose by a yeast strain displaying β-glucosidase on the cell surface
author_sort	Tokuhiro, Kenro
journal	Applied microbiology and biotechnology
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author_browse	Tokuhiro, Kenro Ishida, Nobuhiro Kondo, Akihiko Takahashi, Haruo
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title_sort	lactic fermentation of cellobiose by a yeast strain displaying β-glucosidase on the cell surface
title_auth	Lactic fermentation of cellobiose by a yeast strain displaying β-glucosidase on the cell surface
abstract	Abstract The Aspergillus aculeatus β-glucosidase 1 (bgl1) gene was expressed in a lactic-acid-producing Saccharomyces cerevisiae strain to enable lactic fermentation with cellobiose. The recombinant β-glucosidase enzyme was expressed on the yeast cell surface by fusing the mature protein to the C-terminal half region of the α-agglutinin. The β-glucosidase expression plasmids were integrated into the genome. Three strong promoters of S. cerevisiae, the TDH3, PGK1, and PDC1 promoters, were used for β-glucosidase expression. The specific β-glucosidase activity varied with the promoter used and the copy number of the bgl1 gene. The highest activity was obtained with strain PB2 that possessed two copies of the bgl1 gene driven by the PDC1 promoter. PB2 could grow on cellobiose and glucose minimal medium at the same rate. Fermentation experiments were conducted in non-selective-rich media containing 95 g $ l^{−1} $ cellobiose or 100 g $ l^{−1} $ glucose as a carbon source under microaerobic conditions. The maximum rate of l-lactate production by PB2 on cellobiose (2.8 g $ l^{−1} $ $ h^{−1} $) was similar to that on glucose (3.0 g $ l^{−1} $ $ h^{−1} $). This indicates that efficient fermentation of cellobiose to l-lactate can be accomplished using a yeast strain expressing β-glucosidase from a mitotically stable genomic integration plasmid. © Springer-Verlag 2008
abstractGer	Abstract The Aspergillus aculeatus β-glucosidase 1 (bgl1) gene was expressed in a lactic-acid-producing Saccharomyces cerevisiae strain to enable lactic fermentation with cellobiose. The recombinant β-glucosidase enzyme was expressed on the yeast cell surface by fusing the mature protein to the C-terminal half region of the α-agglutinin. The β-glucosidase expression plasmids were integrated into the genome. Three strong promoters of S. cerevisiae, the TDH3, PGK1, and PDC1 promoters, were used for β-glucosidase expression. The specific β-glucosidase activity varied with the promoter used and the copy number of the bgl1 gene. The highest activity was obtained with strain PB2 that possessed two copies of the bgl1 gene driven by the PDC1 promoter. PB2 could grow on cellobiose and glucose minimal medium at the same rate. Fermentation experiments were conducted in non-selective-rich media containing 95 g $ l^{−1} $ cellobiose or 100 g $ l^{−1} $ glucose as a carbon source under microaerobic conditions. The maximum rate of l-lactate production by PB2 on cellobiose (2.8 g $ l^{−1} $ $ h^{−1} $) was similar to that on glucose (3.0 g $ l^{−1} $ $ h^{−1} $). This indicates that efficient fermentation of cellobiose to l-lactate can be accomplished using a yeast strain expressing β-glucosidase from a mitotically stable genomic integration plasmid. © Springer-Verlag 2008
abstract_unstemmed	Abstract The Aspergillus aculeatus β-glucosidase 1 (bgl1) gene was expressed in a lactic-acid-producing Saccharomyces cerevisiae strain to enable lactic fermentation with cellobiose. The recombinant β-glucosidase enzyme was expressed on the yeast cell surface by fusing the mature protein to the C-terminal half region of the α-agglutinin. The β-glucosidase expression plasmids were integrated into the genome. Three strong promoters of S. cerevisiae, the TDH3, PGK1, and PDC1 promoters, were used for β-glucosidase expression. The specific β-glucosidase activity varied with the promoter used and the copy number of the bgl1 gene. The highest activity was obtained with strain PB2 that possessed two copies of the bgl1 gene driven by the PDC1 promoter. PB2 could grow on cellobiose and glucose minimal medium at the same rate. Fermentation experiments were conducted in non-selective-rich media containing 95 g $ l^{−1} $ cellobiose or 100 g $ l^{−1} $ glucose as a carbon source under microaerobic conditions. The maximum rate of l-lactate production by PB2 on cellobiose (2.8 g $ l^{−1} $ $ h^{−1} $) was similar to that on glucose (3.0 g $ l^{−1} $ $ h^{−1} $). This indicates that efficient fermentation of cellobiose to l-lactate can be accomplished using a yeast strain expressing β-glucosidase from a mitotically stable genomic integration plasmid. © Springer-Verlag 2008
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title_short	Lactic fermentation of cellobiose by a yeast strain displaying β-glucosidase on the cell surface
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Lactic fermentation of cellobiose by a yeast strain displaying β-glucosidase on the cell surface

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