Characterization of binding preference of polyhydroxyalkanoate biosynthesis-related multifunctional protein PhaM from Ralstonia eutropha
Abstract The binding preference of a polyhydroxyalkanoate (PHA) biosynthesis-related multifunctional protein from Ralstonia eutropha ($ PhaM_{Re} $) was characterized. In vitro activity assay showed that PHA synthase from R. eutropha ($ PhaC_{Re} $) was activated by the presence of $ PhaM_{Re} $ but...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Ushimaru, Kazunori [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2016 |
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| Schlagwörter: |
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| Anmerkung: |
© Springer-Verlag Berlin Heidelberg 2016 |
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| Übergeordnetes Werk: |
Enthalten in: Applied microbiology and biotechnology - Berlin : Springer, 1975, 100(2016), 10 vom: 04. Jan., Seite 4413-4421 |
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| Übergeordnetes Werk: |
volume:100 ; year:2016 ; number:10 ; day:04 ; month:01 ; pages:4413-4421 |
| Links: |
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| DOI / URN: |
10.1007/s00253-015-7225-6 |
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| Katalog-ID: |
SPR003008819 |
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| 041 | |a eng | ||
| 100 | 1 | |a Ushimaru, Kazunori |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Characterization of binding preference of polyhydroxyalkanoate biosynthesis-related multifunctional protein PhaM from Ralstonia eutropha |
| 264 | 1 | |c 2016 | |
| 336 | |a Text |b txt |2 rdacontent | ||
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| 500 | |a © Springer-Verlag Berlin Heidelberg 2016 | ||
| 520 | |a Abstract The binding preference of a polyhydroxyalkanoate (PHA) biosynthesis-related multifunctional protein from Ralstonia eutropha ($ PhaM_{Re} $) was characterized. In vitro activity assay showed that PHA synthase from R. eutropha ($ PhaC_{Re} $) was activated by the presence of $ PhaM_{Re} $ but PHA synthase from Aeromonas caviae ($ PhaC_{Ac} $) was not. Additionally, in vitro assays of protein-protein interactions demonstrated that $ PhaM_{Re} $ interacted with $ PhaC_{Re} $ directly, but did not interact with $ PhaC_{Ac} $. These results suggest that the protein-protein interaction is important for the activation of PhaC by $ PhaM_{Re} $. Further analyses indicated that $ PhaM_{Re} $ has little or no direct interaction with the PHA polymer chain. Subsequently, PHA biosynthesis genes (phaARe, phaBRe, and phaCRe/phaCAc) and the phaMRe gene were introduced into recombinant Escherichia coli and cultivated for PHA accumulation. Contrary to our expectations, the expression of $ PhaM_{Re} $ decreased PHA accumulation and changed the morphology of PHA granules to be microscopically obscure shape in $ PhaC_{Re} $-expressing E. coli. No change in the amount of P(3HB) or the morphology of granules by $ PhaM_{Re} $ expression was observed in $ PhaC_{Ac} $-expressing E. coli. These observations suggest that $ PhaM_{Re} $ affects cellular physiology through the PhaM-PhaC interaction. | ||
| 650 | 4 | |a Polyhydroxyalkanoate |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a PHA synthase |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a PhaM |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Protein-protein interaction |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a PHA granule |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Tsuge, Takeharu |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Applied microbiology and biotechnology |d Berlin : Springer, 1975 |g 100(2016), 10 vom: 04. Jan., Seite 4413-4421 |w (DE-627)265509564 |w (DE-600)1464336-4 |x 1432-0614 |7 nnns |
| 773 | 1 | 8 | |g volume:100 |g year:2016 |g number:10 |g day:04 |g month:01 |g pages:4413-4421 |
| 856 | 4 | 0 | |u https://dx.doi.org/10.1007/s00253-015-7225-6 |z lizenzpflichtig |3 Volltext |
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| 912 | |a GBV_ILN_39 | ||
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| 912 | |a GBV_ILN_60 | ||
| 912 | |a GBV_ILN_62 | ||
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| 912 | |a GBV_ILN_73 | ||
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| 912 | |a GBV_ILN_150 | ||
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| 912 | |a GBV_ILN_281 | ||
| 912 | |a GBV_ILN_285 | ||
| 912 | |a GBV_ILN_293 | ||
| 912 | |a GBV_ILN_370 | ||
| 912 | |a GBV_ILN_381 | ||
| 912 | |a GBV_ILN_602 | ||
| 912 | |a GBV_ILN_636 | ||
| 912 | |a GBV_ILN_702 | ||
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| 912 | |a GBV_ILN_2014 | ||
| 912 | |a GBV_ILN_2015 | ||
| 912 | |a GBV_ILN_2020 | ||
| 912 | |a GBV_ILN_2021 | ||
| 912 | |a GBV_ILN_2025 | ||
| 912 | |a GBV_ILN_2026 | ||
| 912 | |a GBV_ILN_2027 | ||
| 912 | |a GBV_ILN_2031 | ||
| 912 | |a GBV_ILN_2034 | ||
| 912 | |a GBV_ILN_2037 | ||
| 912 | |a GBV_ILN_2038 | ||
| 912 | |a GBV_ILN_2039 | ||
| 912 | |a GBV_ILN_2044 | ||
| 912 | |a GBV_ILN_2048 | ||
| 912 | |a GBV_ILN_2049 | ||
| 912 | |a GBV_ILN_2050 | ||
| 912 | |a GBV_ILN_2055 | ||
| 912 | |a GBV_ILN_2056 | ||
| 912 | |a GBV_ILN_2057 | ||
| 912 | |a GBV_ILN_2059 | ||
| 912 | |a GBV_ILN_2061 | ||
| 912 | |a GBV_ILN_2064 | ||
| 912 | |a GBV_ILN_2068 | ||
| 912 | |a GBV_ILN_2070 | ||
| 912 | |a GBV_ILN_2086 | ||
| 912 | |a GBV_ILN_2093 | ||
| 912 | |a GBV_ILN_2106 | ||
| 912 | |a GBV_ILN_2107 | ||
| 912 | |a GBV_ILN_2110 | ||
| 912 | |a GBV_ILN_2113 | ||
| 912 | |a GBV_ILN_2118 | ||
| 912 | |a GBV_ILN_2119 | ||
| 912 | |a GBV_ILN_2129 | ||
| 912 | |a GBV_ILN_2143 | ||
| 912 | |a GBV_ILN_2144 | ||
| 912 | |a GBV_ILN_2147 | ||
| 912 | |a GBV_ILN_2153 | ||
| 912 | |a GBV_ILN_2188 | ||
| 912 | |a GBV_ILN_2232 | ||
| 912 | |a GBV_ILN_2336 | ||
| 912 | |a GBV_ILN_2360 | ||
| 912 | |a GBV_ILN_2446 | ||
| 912 | |a GBV_ILN_2470 | ||
| 912 | |a GBV_ILN_2472 | ||
| 912 | |a GBV_ILN_2507 | ||
| 912 | |a GBV_ILN_2522 | ||
| 912 | |a GBV_ILN_2548 | ||
| 912 | |a GBV_ILN_4012 | ||
| 912 | |a GBV_ILN_4035 | ||
| 912 | |a GBV_ILN_4037 | ||
| 912 | |a GBV_ILN_4046 | ||
| 912 | |a GBV_ILN_4112 | ||
| 912 | |a GBV_ILN_4125 | ||
| 912 | |a GBV_ILN_4126 | ||
| 912 | |a GBV_ILN_4242 | ||
| 912 | |a GBV_ILN_4246 | ||
| 912 | |a GBV_ILN_4249 | ||
| 912 | |a GBV_ILN_4251 | ||
| 912 | |a GBV_ILN_4305 | ||
| 912 | |a GBV_ILN_4306 | ||
| 912 | |a GBV_ILN_4307 | ||
| 912 | |a GBV_ILN_4313 | ||
| 912 | |a GBV_ILN_4322 | ||
| 912 | |a GBV_ILN_4323 | ||
| 912 | |a GBV_ILN_4324 | ||
| 912 | |a GBV_ILN_4326 | ||
| 912 | |a GBV_ILN_4333 | ||
| 912 | |a GBV_ILN_4334 | ||
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| 912 | |a GBV_ILN_4336 | ||
| 912 | |a GBV_ILN_4338 | ||
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| 912 | |a GBV_ILN_4700 | ||
| 951 | |a AR | ||
| 952 | |d 100 |j 2016 |e 10 |b 04 |c 01 |h 4413-4421 | ||
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10.1007/s00253-015-7225-6 doi (DE-627)SPR003008819 (SPR)s00253-015-7225-6-e DE-627 ger DE-627 rakwb eng Ushimaru, Kazunori verfasserin aut Characterization of binding preference of polyhydroxyalkanoate biosynthesis-related multifunctional protein PhaM from Ralstonia eutropha 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag Berlin Heidelberg 2016 Abstract The binding preference of a polyhydroxyalkanoate (PHA) biosynthesis-related multifunctional protein from Ralstonia eutropha ($ PhaM_{Re} $) was characterized. In vitro activity assay showed that PHA synthase from R. eutropha ($ PhaC_{Re} $) was activated by the presence of $ PhaM_{Re} $ but PHA synthase from Aeromonas caviae ($ PhaC_{Ac} $) was not. Additionally, in vitro assays of protein-protein interactions demonstrated that $ PhaM_{Re} $ interacted with $ PhaC_{Re} $ directly, but did not interact with $ PhaC_{Ac} $. These results suggest that the protein-protein interaction is important for the activation of PhaC by $ PhaM_{Re} $. Further analyses indicated that $ PhaM_{Re} $ has little or no direct interaction with the PHA polymer chain. Subsequently, PHA biosynthesis genes (phaARe, phaBRe, and phaCRe/phaCAc) and the phaMRe gene were introduced into recombinant Escherichia coli and cultivated for PHA accumulation. Contrary to our expectations, the expression of $ PhaM_{Re} $ decreased PHA accumulation and changed the morphology of PHA granules to be microscopically obscure shape in $ PhaC_{Re} $-expressing E. coli. No change in the amount of P(3HB) or the morphology of granules by $ PhaM_{Re} $ expression was observed in $ PhaC_{Ac} $-expressing E. coli. These observations suggest that $ PhaM_{Re} $ affects cellular physiology through the PhaM-PhaC interaction. Polyhydroxyalkanoate (dpeaa)DE-He213 PHA synthase (dpeaa)DE-He213 PhaM (dpeaa)DE-He213 Protein-protein interaction (dpeaa)DE-He213 PHA granule (dpeaa)DE-He213 Tsuge, Takeharu aut Enthalten in Applied microbiology and biotechnology Berlin : Springer, 1975 100(2016), 10 vom: 04. Jan., Seite 4413-4421 (DE-627)265509564 (DE-600)1464336-4 1432-0614 nnns volume:100 year:2016 number:10 day:04 month:01 pages:4413-4421 https://dx.doi.org/10.1007/s00253-015-7225-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2110 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2360 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 100 2016 10 04 01 4413-4421 |
| spelling |
10.1007/s00253-015-7225-6 doi (DE-627)SPR003008819 (SPR)s00253-015-7225-6-e DE-627 ger DE-627 rakwb eng Ushimaru, Kazunori verfasserin aut Characterization of binding preference of polyhydroxyalkanoate biosynthesis-related multifunctional protein PhaM from Ralstonia eutropha 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag Berlin Heidelberg 2016 Abstract The binding preference of a polyhydroxyalkanoate (PHA) biosynthesis-related multifunctional protein from Ralstonia eutropha ($ PhaM_{Re} $) was characterized. In vitro activity assay showed that PHA synthase from R. eutropha ($ PhaC_{Re} $) was activated by the presence of $ PhaM_{Re} $ but PHA synthase from Aeromonas caviae ($ PhaC_{Ac} $) was not. Additionally, in vitro assays of protein-protein interactions demonstrated that $ PhaM_{Re} $ interacted with $ PhaC_{Re} $ directly, but did not interact with $ PhaC_{Ac} $. These results suggest that the protein-protein interaction is important for the activation of PhaC by $ PhaM_{Re} $. Further analyses indicated that $ PhaM_{Re} $ has little or no direct interaction with the PHA polymer chain. Subsequently, PHA biosynthesis genes (phaARe, phaBRe, and phaCRe/phaCAc) and the phaMRe gene were introduced into recombinant Escherichia coli and cultivated for PHA accumulation. Contrary to our expectations, the expression of $ PhaM_{Re} $ decreased PHA accumulation and changed the morphology of PHA granules to be microscopically obscure shape in $ PhaC_{Re} $-expressing E. coli. No change in the amount of P(3HB) or the morphology of granules by $ PhaM_{Re} $ expression was observed in $ PhaC_{Ac} $-expressing E. coli. These observations suggest that $ PhaM_{Re} $ affects cellular physiology through the PhaM-PhaC interaction. Polyhydroxyalkanoate (dpeaa)DE-He213 PHA synthase (dpeaa)DE-He213 PhaM (dpeaa)DE-He213 Protein-protein interaction (dpeaa)DE-He213 PHA granule (dpeaa)DE-He213 Tsuge, Takeharu aut Enthalten in Applied microbiology and biotechnology Berlin : Springer, 1975 100(2016), 10 vom: 04. Jan., Seite 4413-4421 (DE-627)265509564 (DE-600)1464336-4 1432-0614 nnns volume:100 year:2016 number:10 day:04 month:01 pages:4413-4421 https://dx.doi.org/10.1007/s00253-015-7225-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2110 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2360 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 100 2016 10 04 01 4413-4421 |
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10.1007/s00253-015-7225-6 doi (DE-627)SPR003008819 (SPR)s00253-015-7225-6-e DE-627 ger DE-627 rakwb eng Ushimaru, Kazunori verfasserin aut Characterization of binding preference of polyhydroxyalkanoate biosynthesis-related multifunctional protein PhaM from Ralstonia eutropha 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag Berlin Heidelberg 2016 Abstract The binding preference of a polyhydroxyalkanoate (PHA) biosynthesis-related multifunctional protein from Ralstonia eutropha ($ PhaM_{Re} $) was characterized. In vitro activity assay showed that PHA synthase from R. eutropha ($ PhaC_{Re} $) was activated by the presence of $ PhaM_{Re} $ but PHA synthase from Aeromonas caviae ($ PhaC_{Ac} $) was not. Additionally, in vitro assays of protein-protein interactions demonstrated that $ PhaM_{Re} $ interacted with $ PhaC_{Re} $ directly, but did not interact with $ PhaC_{Ac} $. These results suggest that the protein-protein interaction is important for the activation of PhaC by $ PhaM_{Re} $. Further analyses indicated that $ PhaM_{Re} $ has little or no direct interaction with the PHA polymer chain. Subsequently, PHA biosynthesis genes (phaARe, phaBRe, and phaCRe/phaCAc) and the phaMRe gene were introduced into recombinant Escherichia coli and cultivated for PHA accumulation. Contrary to our expectations, the expression of $ PhaM_{Re} $ decreased PHA accumulation and changed the morphology of PHA granules to be microscopically obscure shape in $ PhaC_{Re} $-expressing E. coli. No change in the amount of P(3HB) or the morphology of granules by $ PhaM_{Re} $ expression was observed in $ PhaC_{Ac} $-expressing E. coli. These observations suggest that $ PhaM_{Re} $ affects cellular physiology through the PhaM-PhaC interaction. Polyhydroxyalkanoate (dpeaa)DE-He213 PHA synthase (dpeaa)DE-He213 PhaM (dpeaa)DE-He213 Protein-protein interaction (dpeaa)DE-He213 PHA granule (dpeaa)DE-He213 Tsuge, Takeharu aut Enthalten in Applied microbiology and biotechnology Berlin : Springer, 1975 100(2016), 10 vom: 04. Jan., Seite 4413-4421 (DE-627)265509564 (DE-600)1464336-4 1432-0614 nnns volume:100 year:2016 number:10 day:04 month:01 pages:4413-4421 https://dx.doi.org/10.1007/s00253-015-7225-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2110 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2360 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 100 2016 10 04 01 4413-4421 |
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10.1007/s00253-015-7225-6 doi (DE-627)SPR003008819 (SPR)s00253-015-7225-6-e DE-627 ger DE-627 rakwb eng Ushimaru, Kazunori verfasserin aut Characterization of binding preference of polyhydroxyalkanoate biosynthesis-related multifunctional protein PhaM from Ralstonia eutropha 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag Berlin Heidelberg 2016 Abstract The binding preference of a polyhydroxyalkanoate (PHA) biosynthesis-related multifunctional protein from Ralstonia eutropha ($ PhaM_{Re} $) was characterized. In vitro activity assay showed that PHA synthase from R. eutropha ($ PhaC_{Re} $) was activated by the presence of $ PhaM_{Re} $ but PHA synthase from Aeromonas caviae ($ PhaC_{Ac} $) was not. Additionally, in vitro assays of protein-protein interactions demonstrated that $ PhaM_{Re} $ interacted with $ PhaC_{Re} $ directly, but did not interact with $ PhaC_{Ac} $. These results suggest that the protein-protein interaction is important for the activation of PhaC by $ PhaM_{Re} $. Further analyses indicated that $ PhaM_{Re} $ has little or no direct interaction with the PHA polymer chain. Subsequently, PHA biosynthesis genes (phaARe, phaBRe, and phaCRe/phaCAc) and the phaMRe gene were introduced into recombinant Escherichia coli and cultivated for PHA accumulation. Contrary to our expectations, the expression of $ PhaM_{Re} $ decreased PHA accumulation and changed the morphology of PHA granules to be microscopically obscure shape in $ PhaC_{Re} $-expressing E. coli. No change in the amount of P(3HB) or the morphology of granules by $ PhaM_{Re} $ expression was observed in $ PhaC_{Ac} $-expressing E. coli. These observations suggest that $ PhaM_{Re} $ affects cellular physiology through the PhaM-PhaC interaction. Polyhydroxyalkanoate (dpeaa)DE-He213 PHA synthase (dpeaa)DE-He213 PhaM (dpeaa)DE-He213 Protein-protein interaction (dpeaa)DE-He213 PHA granule (dpeaa)DE-He213 Tsuge, Takeharu aut Enthalten in Applied microbiology and biotechnology Berlin : Springer, 1975 100(2016), 10 vom: 04. Jan., Seite 4413-4421 (DE-627)265509564 (DE-600)1464336-4 1432-0614 nnns volume:100 year:2016 number:10 day:04 month:01 pages:4413-4421 https://dx.doi.org/10.1007/s00253-015-7225-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2110 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2360 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 100 2016 10 04 01 4413-4421 |
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10.1007/s00253-015-7225-6 doi (DE-627)SPR003008819 (SPR)s00253-015-7225-6-e DE-627 ger DE-627 rakwb eng Ushimaru, Kazunori verfasserin aut Characterization of binding preference of polyhydroxyalkanoate biosynthesis-related multifunctional protein PhaM from Ralstonia eutropha 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag Berlin Heidelberg 2016 Abstract The binding preference of a polyhydroxyalkanoate (PHA) biosynthesis-related multifunctional protein from Ralstonia eutropha ($ PhaM_{Re} $) was characterized. In vitro activity assay showed that PHA synthase from R. eutropha ($ PhaC_{Re} $) was activated by the presence of $ PhaM_{Re} $ but PHA synthase from Aeromonas caviae ($ PhaC_{Ac} $) was not. Additionally, in vitro assays of protein-protein interactions demonstrated that $ PhaM_{Re} $ interacted with $ PhaC_{Re} $ directly, but did not interact with $ PhaC_{Ac} $. These results suggest that the protein-protein interaction is important for the activation of PhaC by $ PhaM_{Re} $. Further analyses indicated that $ PhaM_{Re} $ has little or no direct interaction with the PHA polymer chain. Subsequently, PHA biosynthesis genes (phaARe, phaBRe, and phaCRe/phaCAc) and the phaMRe gene were introduced into recombinant Escherichia coli and cultivated for PHA accumulation. Contrary to our expectations, the expression of $ PhaM_{Re} $ decreased PHA accumulation and changed the morphology of PHA granules to be microscopically obscure shape in $ PhaC_{Re} $-expressing E. coli. No change in the amount of P(3HB) or the morphology of granules by $ PhaM_{Re} $ expression was observed in $ PhaC_{Ac} $-expressing E. coli. These observations suggest that $ PhaM_{Re} $ affects cellular physiology through the PhaM-PhaC interaction. Polyhydroxyalkanoate (dpeaa)DE-He213 PHA synthase (dpeaa)DE-He213 PhaM (dpeaa)DE-He213 Protein-protein interaction (dpeaa)DE-He213 PHA granule (dpeaa)DE-He213 Tsuge, Takeharu aut Enthalten in Applied microbiology and biotechnology Berlin : Springer, 1975 100(2016), 10 vom: 04. Jan., Seite 4413-4421 (DE-627)265509564 (DE-600)1464336-4 1432-0614 nnns volume:100 year:2016 number:10 day:04 month:01 pages:4413-4421 https://dx.doi.org/10.1007/s00253-015-7225-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2110 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2360 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 100 2016 10 04 01 4413-4421 |
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English |
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Enthalten in Applied microbiology and biotechnology 100(2016), 10 vom: 04. Jan., Seite 4413-4421 volume:100 year:2016 number:10 day:04 month:01 pages:4413-4421 |
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Enthalten in Applied microbiology and biotechnology 100(2016), 10 vom: 04. Jan., Seite 4413-4421 volume:100 year:2016 number:10 day:04 month:01 pages:4413-4421 |
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Polyhydroxyalkanoate PHA synthase PhaM Protein-protein interaction PHA granule |
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Applied microbiology and biotechnology |
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Ushimaru, Kazunori @@aut@@ Tsuge, Takeharu @@aut@@ |
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2016-01-04T00:00:00Z |
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In vitro activity assay showed that PHA synthase from R. eutropha ($ PhaC_{Re} $) was activated by the presence of $ PhaM_{Re} $ but PHA synthase from Aeromonas caviae ($ PhaC_{Ac} $) was not. Additionally, in vitro assays of protein-protein interactions demonstrated that $ PhaM_{Re} $ interacted with $ PhaC_{Re} $ directly, but did not interact with $ PhaC_{Ac} $. These results suggest that the protein-protein interaction is important for the activation of PhaC by $ PhaM_{Re} $. Further analyses indicated that $ PhaM_{Re} $ has little or no direct interaction with the PHA polymer chain. Subsequently, PHA biosynthesis genes (phaARe, phaBRe, and phaCRe/phaCAc) and the phaMRe gene were introduced into recombinant Escherichia coli and cultivated for PHA accumulation. Contrary to our expectations, the expression of $ PhaM_{Re} $ decreased PHA accumulation and changed the morphology of PHA granules to be microscopically obscure shape in $ PhaC_{Re} $-expressing E. coli. No change in the amount of P(3HB) or the morphology of granules by $ PhaM_{Re} $ expression was observed in $ PhaC_{Ac} $-expressing E. coli. 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| author |
Ushimaru, Kazunori |
| spellingShingle |
Ushimaru, Kazunori misc Polyhydroxyalkanoate misc PHA synthase misc PhaM misc Protein-protein interaction misc PHA granule Characterization of binding preference of polyhydroxyalkanoate biosynthesis-related multifunctional protein PhaM from Ralstonia eutropha |
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Characterization of binding preference of polyhydroxyalkanoate biosynthesis-related multifunctional protein PhaM from Ralstonia eutropha Polyhydroxyalkanoate (dpeaa)DE-He213 PHA synthase (dpeaa)DE-He213 PhaM (dpeaa)DE-He213 Protein-protein interaction (dpeaa)DE-He213 PHA granule (dpeaa)DE-He213 |
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misc Polyhydroxyalkanoate misc PHA synthase misc PhaM misc Protein-protein interaction misc PHA granule |
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misc Polyhydroxyalkanoate misc PHA synthase misc PhaM misc Protein-protein interaction misc PHA granule |
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Characterization of binding preference of polyhydroxyalkanoate biosynthesis-related multifunctional protein PhaM from Ralstonia eutropha |
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Characterization of binding preference of polyhydroxyalkanoate biosynthesis-related multifunctional protein PhaM from Ralstonia eutropha |
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Ushimaru, Kazunori |
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Applied microbiology and biotechnology |
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Ushimaru, Kazunori Tsuge, Takeharu |
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Ushimaru, Kazunori |
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10.1007/s00253-015-7225-6 |
| title_sort |
characterization of binding preference of polyhydroxyalkanoate biosynthesis-related multifunctional protein pham from ralstonia eutropha |
| title_auth |
Characterization of binding preference of polyhydroxyalkanoate biosynthesis-related multifunctional protein PhaM from Ralstonia eutropha |
| abstract |
Abstract The binding preference of a polyhydroxyalkanoate (PHA) biosynthesis-related multifunctional protein from Ralstonia eutropha ($ PhaM_{Re} $) was characterized. In vitro activity assay showed that PHA synthase from R. eutropha ($ PhaC_{Re} $) was activated by the presence of $ PhaM_{Re} $ but PHA synthase from Aeromonas caviae ($ PhaC_{Ac} $) was not. Additionally, in vitro assays of protein-protein interactions demonstrated that $ PhaM_{Re} $ interacted with $ PhaC_{Re} $ directly, but did not interact with $ PhaC_{Ac} $. These results suggest that the protein-protein interaction is important for the activation of PhaC by $ PhaM_{Re} $. Further analyses indicated that $ PhaM_{Re} $ has little or no direct interaction with the PHA polymer chain. Subsequently, PHA biosynthesis genes (phaARe, phaBRe, and phaCRe/phaCAc) and the phaMRe gene were introduced into recombinant Escherichia coli and cultivated for PHA accumulation. Contrary to our expectations, the expression of $ PhaM_{Re} $ decreased PHA accumulation and changed the morphology of PHA granules to be microscopically obscure shape in $ PhaC_{Re} $-expressing E. coli. No change in the amount of P(3HB) or the morphology of granules by $ PhaM_{Re} $ expression was observed in $ PhaC_{Ac} $-expressing E. coli. These observations suggest that $ PhaM_{Re} $ affects cellular physiology through the PhaM-PhaC interaction. © Springer-Verlag Berlin Heidelberg 2016 |
| abstractGer |
Abstract The binding preference of a polyhydroxyalkanoate (PHA) biosynthesis-related multifunctional protein from Ralstonia eutropha ($ PhaM_{Re} $) was characterized. In vitro activity assay showed that PHA synthase from R. eutropha ($ PhaC_{Re} $) was activated by the presence of $ PhaM_{Re} $ but PHA synthase from Aeromonas caviae ($ PhaC_{Ac} $) was not. Additionally, in vitro assays of protein-protein interactions demonstrated that $ PhaM_{Re} $ interacted with $ PhaC_{Re} $ directly, but did not interact with $ PhaC_{Ac} $. These results suggest that the protein-protein interaction is important for the activation of PhaC by $ PhaM_{Re} $. Further analyses indicated that $ PhaM_{Re} $ has little or no direct interaction with the PHA polymer chain. Subsequently, PHA biosynthesis genes (phaARe, phaBRe, and phaCRe/phaCAc) and the phaMRe gene were introduced into recombinant Escherichia coli and cultivated for PHA accumulation. Contrary to our expectations, the expression of $ PhaM_{Re} $ decreased PHA accumulation and changed the morphology of PHA granules to be microscopically obscure shape in $ PhaC_{Re} $-expressing E. coli. No change in the amount of P(3HB) or the morphology of granules by $ PhaM_{Re} $ expression was observed in $ PhaC_{Ac} $-expressing E. coli. These observations suggest that $ PhaM_{Re} $ affects cellular physiology through the PhaM-PhaC interaction. © Springer-Verlag Berlin Heidelberg 2016 |
| abstract_unstemmed |
Abstract The binding preference of a polyhydroxyalkanoate (PHA) biosynthesis-related multifunctional protein from Ralstonia eutropha ($ PhaM_{Re} $) was characterized. In vitro activity assay showed that PHA synthase from R. eutropha ($ PhaC_{Re} $) was activated by the presence of $ PhaM_{Re} $ but PHA synthase from Aeromonas caviae ($ PhaC_{Ac} $) was not. Additionally, in vitro assays of protein-protein interactions demonstrated that $ PhaM_{Re} $ interacted with $ PhaC_{Re} $ directly, but did not interact with $ PhaC_{Ac} $. These results suggest that the protein-protein interaction is important for the activation of PhaC by $ PhaM_{Re} $. Further analyses indicated that $ PhaM_{Re} $ has little or no direct interaction with the PHA polymer chain. Subsequently, PHA biosynthesis genes (phaARe, phaBRe, and phaCRe/phaCAc) and the phaMRe gene were introduced into recombinant Escherichia coli and cultivated for PHA accumulation. Contrary to our expectations, the expression of $ PhaM_{Re} $ decreased PHA accumulation and changed the morphology of PHA granules to be microscopically obscure shape in $ PhaC_{Re} $-expressing E. coli. No change in the amount of P(3HB) or the morphology of granules by $ PhaM_{Re} $ expression was observed in $ PhaC_{Ac} $-expressing E. coli. These observations suggest that $ PhaM_{Re} $ affects cellular physiology through the PhaM-PhaC interaction. © Springer-Verlag Berlin Heidelberg 2016 |
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10 |
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Characterization of binding preference of polyhydroxyalkanoate biosynthesis-related multifunctional protein PhaM from Ralstonia eutropha |
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https://dx.doi.org/10.1007/s00253-015-7225-6 |
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Tsuge, Takeharu |
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10.1007/s00253-015-7225-6 |
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2024-07-03T16:42:05.714Z |
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| score |
7.3999043 |