Quantification of the catalytic performance of C1-cellulose-specific lytic polysaccharide monooxygenases

Abstract Lytic polysaccharide monooxygenases (LPMOs) have recently been shown to significantly enhance the degradation of recalcitrant polysaccharides and are of interest for the production of biochemicals and bioethanol from plant biomass. The copper-containing LPMOs utilize electrons, provided by...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Frommhagen, Matthias [verfasserIn] Westphal, Adrie H. Hilgers, Roelant Koetsier, Martijn J. Hinz, Sandra W. A. Visser, Jaap Gruppen, Harry van Berkel, Willem J. H. Kabel, Mirjam A.

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2017

Schlagwörter:	β-Glucosidase Lignocellulose Lytic polysaccharide monooxygenase Plant biomass Reducing agent

Anmerkung:	© The Author(s) 2017

Übergeordnetes Werk:	Enthalten in: Applied microbiology and biotechnology - Berlin : Springer, 1975, 102(2017), 3 vom: 02. Dez., Seite 1281-1295
Übergeordnetes Werk:	volume:102 ; year:2017 ; number:3 ; day:02 ; month:12 ; pages:1281-1295

Links:	Volltext

DOI / URN:	10.1007/s00253-017-8541-9

Katalog-ID:	SPR003030466

Internformat


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100	1		\|a Frommhagen, Matthias \|e verfasserin \|4 aut
245	1	0	\|a Quantification of the catalytic performance of C1-cellulose-specific lytic polysaccharide monooxygenases
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520			\|a Abstract Lytic polysaccharide monooxygenases (LPMOs) have recently been shown to significantly enhance the degradation of recalcitrant polysaccharides and are of interest for the production of biochemicals and bioethanol from plant biomass. The copper-containing LPMOs utilize electrons, provided by reducing agents, to oxidatively cleave polysaccharides. Here, we report the development of a β-glucosidase-assisted method to quantify the release of C1-oxidized gluco-oligosaccharides from cellulose by two C1-oxidizing LPMOs from Myceliophthora thermophila C1. Based on this quantification method, we demonstrate that the catalytic performance of both MtLPMOs is strongly dependent on pH and temperature. The obtained results indicate that the catalytic performance of LPMOs depends on the interaction of multiple factors, which are affected by both pH and temperature.
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650		4	\|a Lignocellulose \|7 (dpeaa)DE-He213
650		4	\|a Lytic polysaccharide monooxygenase \|7 (dpeaa)DE-He213
650		4	\|a Plant biomass \|7 (dpeaa)DE-He213
650		4	\|a Reducing agent \|7 (dpeaa)DE-He213
700	1		\|a Westphal, Adrie H. \|4 aut
700	1		\|a Hilgers, Roelant \|4 aut
700	1		\|a Koetsier, Martijn J. \|4 aut
700	1		\|a Hinz, Sandra W. A. \|4 aut
700	1		\|a Visser, Jaap \|4 aut
700	1		\|a Gruppen, Harry \|4 aut
700	1		\|a van Berkel, Willem J. H. \|4 aut
700	1		\|a Kabel, Mirjam A. \|0 (orcid)0000-0002-2787-7852 \|4 aut
773	0	8	\|i Enthalten in \|t Applied microbiology and biotechnology \|d Berlin : Springer, 1975 \|g 102(2017), 3 vom: 02. Dez., Seite 1281-1295 \|w (DE-627)265509564 \|w (DE-600)1464336-4 \|x 1432-0614 \|7 nnns
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912			\|a GBV_USEFLAG_A
912			\|a SYSFLAG_A
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912			\|a GBV_ILN_11
912			\|a GBV_ILN_20
912			\|a GBV_ILN_22
912			\|a GBV_ILN_23
912			\|a GBV_ILN_24
912			\|a GBV_ILN_31
912			\|a GBV_ILN_32
912			\|a GBV_ILN_39
912			\|a GBV_ILN_40
912			\|a GBV_ILN_60
912			\|a GBV_ILN_62
912			\|a GBV_ILN_63
912			\|a GBV_ILN_69
912			\|a GBV_ILN_70
912			\|a GBV_ILN_73
912			\|a GBV_ILN_74
912			\|a GBV_ILN_90
912			\|a GBV_ILN_95
912			\|a GBV_ILN_100
912			\|a GBV_ILN_101
912			\|a GBV_ILN_105
912			\|a GBV_ILN_110
912			\|a GBV_ILN_120
912			\|a GBV_ILN_138
912			\|a GBV_ILN_150
912			\|a GBV_ILN_151
912			\|a GBV_ILN_152
912			\|a GBV_ILN_161
912			\|a GBV_ILN_165
912			\|a GBV_ILN_170
912			\|a GBV_ILN_171
912			\|a GBV_ILN_187
912			\|a GBV_ILN_206
912			\|a GBV_ILN_213
912			\|a GBV_ILN_224
912			\|a GBV_ILN_230
912			\|a GBV_ILN_250
912			\|a GBV_ILN_267
912			\|a GBV_ILN_281
912			\|a GBV_ILN_285
912			\|a GBV_ILN_293
912			\|a GBV_ILN_370
912			\|a GBV_ILN_381
912			\|a GBV_ILN_602
912			\|a GBV_ILN_636
912			\|a GBV_ILN_702
912			\|a GBV_ILN_2001
912			\|a GBV_ILN_2003
912			\|a GBV_ILN_2004
912			\|a GBV_ILN_2005
912			\|a GBV_ILN_2006
912			\|a GBV_ILN_2007
912			\|a GBV_ILN_2008
912			\|a GBV_ILN_2009
912			\|a GBV_ILN_2010
912			\|a GBV_ILN_2011
912			\|a GBV_ILN_2014
912			\|a GBV_ILN_2015
912			\|a GBV_ILN_2020
912			\|a GBV_ILN_2021
912			\|a GBV_ILN_2025
912			\|a GBV_ILN_2026
912			\|a GBV_ILN_2027
912			\|a GBV_ILN_2031
912			\|a GBV_ILN_2034
912			\|a GBV_ILN_2037
912			\|a GBV_ILN_2038
912			\|a GBV_ILN_2039
912			\|a GBV_ILN_2044
912			\|a GBV_ILN_2048
912			\|a GBV_ILN_2049
912			\|a GBV_ILN_2050
912			\|a GBV_ILN_2055
912			\|a GBV_ILN_2056
912			\|a GBV_ILN_2057
912			\|a GBV_ILN_2059
912			\|a GBV_ILN_2061
912			\|a GBV_ILN_2064
912			\|a GBV_ILN_2068
912			\|a GBV_ILN_2070
912			\|a GBV_ILN_2086
912			\|a GBV_ILN_2093
912			\|a GBV_ILN_2106
912			\|a GBV_ILN_2107
912			\|a GBV_ILN_2110
912			\|a GBV_ILN_2113
912			\|a GBV_ILN_2118
912			\|a GBV_ILN_2119
912			\|a GBV_ILN_2129
912			\|a GBV_ILN_2143
912			\|a GBV_ILN_2144
912			\|a GBV_ILN_2147
912			\|a GBV_ILN_2153
912			\|a GBV_ILN_2188
912			\|a GBV_ILN_2232
912			\|a GBV_ILN_2336
912			\|a GBV_ILN_2360
912			\|a GBV_ILN_2446
912			\|a GBV_ILN_2470
912			\|a GBV_ILN_2472
912			\|a GBV_ILN_2507
912			\|a GBV_ILN_2522
912			\|a GBV_ILN_2548
912			\|a GBV_ILN_4012
912			\|a GBV_ILN_4035
912			\|a GBV_ILN_4037
912			\|a GBV_ILN_4046
912			\|a GBV_ILN_4112
912			\|a GBV_ILN_4125
912			\|a GBV_ILN_4126
912			\|a GBV_ILN_4242
912			\|a GBV_ILN_4246
912			\|a GBV_ILN_4249
912			\|a GBV_ILN_4251
912			\|a GBV_ILN_4305
912			\|a GBV_ILN_4306
912			\|a GBV_ILN_4307
912			\|a GBV_ILN_4313
912			\|a GBV_ILN_4322
912			\|a GBV_ILN_4323
912			\|a GBV_ILN_4324
912			\|a GBV_ILN_4326
912			\|a GBV_ILN_4333
912			\|a GBV_ILN_4334
912			\|a GBV_ILN_4335
912			\|a GBV_ILN_4336
912			\|a GBV_ILN_4338
912			\|a GBV_ILN_4393
912			\|a GBV_ILN_4700
951			\|a AR
952			\|d 102 \|j 2017 \|e 3 \|b 02 \|c 12 \|h 1281-1295

Indexfelder

author_variant	m f mf a h w ah ahw r h rh m j k mj mjk s w a h swa swah j v jv h g hg b w j h v bwjh bwjhv m a k ma mak
matchkey_str	article:14320614:2017----::uniiainfhctltcefracoccluoepcfcyipl
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publishDate	2017
allfields	10.1007/s00253-017-8541-9 doi (DE-627)SPR003030466 (SPR)s00253-017-8541-9-e DE-627 ger DE-627 rakwb eng Frommhagen, Matthias verfasserin aut Quantification of the catalytic performance of C1-cellulose-specific lytic polysaccharide monooxygenases 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2017 Abstract Lytic polysaccharide monooxygenases (LPMOs) have recently been shown to significantly enhance the degradation of recalcitrant polysaccharides and are of interest for the production of biochemicals and bioethanol from plant biomass. The copper-containing LPMOs utilize electrons, provided by reducing agents, to oxidatively cleave polysaccharides. Here, we report the development of a β-glucosidase-assisted method to quantify the release of C1-oxidized gluco-oligosaccharides from cellulose by two C1-oxidizing LPMOs from Myceliophthora thermophila C1. Based on this quantification method, we demonstrate that the catalytic performance of both MtLPMOs is strongly dependent on pH and temperature. The obtained results indicate that the catalytic performance of LPMOs depends on the interaction of multiple factors, which are affected by both pH and temperature. β-Glucosidase (dpeaa)DE-He213 Lignocellulose (dpeaa)DE-He213 Lytic polysaccharide monooxygenase (dpeaa)DE-He213 Plant biomass (dpeaa)DE-He213 Reducing agent (dpeaa)DE-He213 Westphal, Adrie H. aut Hilgers, Roelant aut Koetsier, Martijn J. aut Hinz, Sandra W. A. aut Visser, Jaap aut Gruppen, Harry aut van Berkel, Willem J. H. aut Kabel, Mirjam A. (orcid)0000-0002-2787-7852 aut Enthalten in Applied microbiology and biotechnology Berlin : Springer, 1975 102(2017), 3 vom: 02. Dez., Seite 1281-1295 (DE-627)265509564 (DE-600)1464336-4 1432-0614 nnns volume:102 year:2017 number:3 day:02 month:12 pages:1281-1295 https://dx.doi.org/10.1007/s00253-017-8541-9 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2110 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2360 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 102 2017 3 02 12 1281-1295
spelling	10.1007/s00253-017-8541-9 doi (DE-627)SPR003030466 (SPR)s00253-017-8541-9-e DE-627 ger DE-627 rakwb eng Frommhagen, Matthias verfasserin aut Quantification of the catalytic performance of C1-cellulose-specific lytic polysaccharide monooxygenases 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2017 Abstract Lytic polysaccharide monooxygenases (LPMOs) have recently been shown to significantly enhance the degradation of recalcitrant polysaccharides and are of interest for the production of biochemicals and bioethanol from plant biomass. The copper-containing LPMOs utilize electrons, provided by reducing agents, to oxidatively cleave polysaccharides. Here, we report the development of a β-glucosidase-assisted method to quantify the release of C1-oxidized gluco-oligosaccharides from cellulose by two C1-oxidizing LPMOs from Myceliophthora thermophila C1. Based on this quantification method, we demonstrate that the catalytic performance of both MtLPMOs is strongly dependent on pH and temperature. The obtained results indicate that the catalytic performance of LPMOs depends on the interaction of multiple factors, which are affected by both pH and temperature. β-Glucosidase (dpeaa)DE-He213 Lignocellulose (dpeaa)DE-He213 Lytic polysaccharide monooxygenase (dpeaa)DE-He213 Plant biomass (dpeaa)DE-He213 Reducing agent (dpeaa)DE-He213 Westphal, Adrie H. aut Hilgers, Roelant aut Koetsier, Martijn J. aut Hinz, Sandra W. A. aut Visser, Jaap aut Gruppen, Harry aut van Berkel, Willem J. H. aut Kabel, Mirjam A. (orcid)0000-0002-2787-7852 aut Enthalten in Applied microbiology and biotechnology Berlin : Springer, 1975 102(2017), 3 vom: 02. Dez., Seite 1281-1295 (DE-627)265509564 (DE-600)1464336-4 1432-0614 nnns volume:102 year:2017 number:3 day:02 month:12 pages:1281-1295 https://dx.doi.org/10.1007/s00253-017-8541-9 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2110 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2360 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 102 2017 3 02 12 1281-1295
allfields_unstemmed	10.1007/s00253-017-8541-9 doi (DE-627)SPR003030466 (SPR)s00253-017-8541-9-e DE-627 ger DE-627 rakwb eng Frommhagen, Matthias verfasserin aut Quantification of the catalytic performance of C1-cellulose-specific lytic polysaccharide monooxygenases 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2017 Abstract Lytic polysaccharide monooxygenases (LPMOs) have recently been shown to significantly enhance the degradation of recalcitrant polysaccharides and are of interest for the production of biochemicals and bioethanol from plant biomass. The copper-containing LPMOs utilize electrons, provided by reducing agents, to oxidatively cleave polysaccharides. Here, we report the development of a β-glucosidase-assisted method to quantify the release of C1-oxidized gluco-oligosaccharides from cellulose by two C1-oxidizing LPMOs from Myceliophthora thermophila C1. Based on this quantification method, we demonstrate that the catalytic performance of both MtLPMOs is strongly dependent on pH and temperature. The obtained results indicate that the catalytic performance of LPMOs depends on the interaction of multiple factors, which are affected by both pH and temperature. β-Glucosidase (dpeaa)DE-He213 Lignocellulose (dpeaa)DE-He213 Lytic polysaccharide monooxygenase (dpeaa)DE-He213 Plant biomass (dpeaa)DE-He213 Reducing agent (dpeaa)DE-He213 Westphal, Adrie H. aut Hilgers, Roelant aut Koetsier, Martijn J. aut Hinz, Sandra W. A. aut Visser, Jaap aut Gruppen, Harry aut van Berkel, Willem J. H. aut Kabel, Mirjam A. (orcid)0000-0002-2787-7852 aut Enthalten in Applied microbiology and biotechnology Berlin : Springer, 1975 102(2017), 3 vom: 02. Dez., Seite 1281-1295 (DE-627)265509564 (DE-600)1464336-4 1432-0614 nnns volume:102 year:2017 number:3 day:02 month:12 pages:1281-1295 https://dx.doi.org/10.1007/s00253-017-8541-9 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2110 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2360 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 102 2017 3 02 12 1281-1295
allfieldsGer	10.1007/s00253-017-8541-9 doi (DE-627)SPR003030466 (SPR)s00253-017-8541-9-e DE-627 ger DE-627 rakwb eng Frommhagen, Matthias verfasserin aut Quantification of the catalytic performance of C1-cellulose-specific lytic polysaccharide monooxygenases 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2017 Abstract Lytic polysaccharide monooxygenases (LPMOs) have recently been shown to significantly enhance the degradation of recalcitrant polysaccharides and are of interest for the production of biochemicals and bioethanol from plant biomass. The copper-containing LPMOs utilize electrons, provided by reducing agents, to oxidatively cleave polysaccharides. Here, we report the development of a β-glucosidase-assisted method to quantify the release of C1-oxidized gluco-oligosaccharides from cellulose by two C1-oxidizing LPMOs from Myceliophthora thermophila C1. Based on this quantification method, we demonstrate that the catalytic performance of both MtLPMOs is strongly dependent on pH and temperature. The obtained results indicate that the catalytic performance of LPMOs depends on the interaction of multiple factors, which are affected by both pH and temperature. β-Glucosidase (dpeaa)DE-He213 Lignocellulose (dpeaa)DE-He213 Lytic polysaccharide monooxygenase (dpeaa)DE-He213 Plant biomass (dpeaa)DE-He213 Reducing agent (dpeaa)DE-He213 Westphal, Adrie H. aut Hilgers, Roelant aut Koetsier, Martijn J. aut Hinz, Sandra W. A. aut Visser, Jaap aut Gruppen, Harry aut van Berkel, Willem J. H. aut Kabel, Mirjam A. (orcid)0000-0002-2787-7852 aut Enthalten in Applied microbiology and biotechnology Berlin : Springer, 1975 102(2017), 3 vom: 02. Dez., Seite 1281-1295 (DE-627)265509564 (DE-600)1464336-4 1432-0614 nnns volume:102 year:2017 number:3 day:02 month:12 pages:1281-1295 https://dx.doi.org/10.1007/s00253-017-8541-9 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2110 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2360 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 102 2017 3 02 12 1281-1295
allfieldsSound	10.1007/s00253-017-8541-9 doi (DE-627)SPR003030466 (SPR)s00253-017-8541-9-e DE-627 ger DE-627 rakwb eng Frommhagen, Matthias verfasserin aut Quantification of the catalytic performance of C1-cellulose-specific lytic polysaccharide monooxygenases 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2017 Abstract Lytic polysaccharide monooxygenases (LPMOs) have recently been shown to significantly enhance the degradation of recalcitrant polysaccharides and are of interest for the production of biochemicals and bioethanol from plant biomass. The copper-containing LPMOs utilize electrons, provided by reducing agents, to oxidatively cleave polysaccharides. Here, we report the development of a β-glucosidase-assisted method to quantify the release of C1-oxidized gluco-oligosaccharides from cellulose by two C1-oxidizing LPMOs from Myceliophthora thermophila C1. Based on this quantification method, we demonstrate that the catalytic performance of both MtLPMOs is strongly dependent on pH and temperature. The obtained results indicate that the catalytic performance of LPMOs depends on the interaction of multiple factors, which are affected by both pH and temperature. β-Glucosidase (dpeaa)DE-He213 Lignocellulose (dpeaa)DE-He213 Lytic polysaccharide monooxygenase (dpeaa)DE-He213 Plant biomass (dpeaa)DE-He213 Reducing agent (dpeaa)DE-He213 Westphal, Adrie H. aut Hilgers, Roelant aut Koetsier, Martijn J. aut Hinz, Sandra W. A. aut Visser, Jaap aut Gruppen, Harry aut van Berkel, Willem J. H. aut Kabel, Mirjam A. (orcid)0000-0002-2787-7852 aut Enthalten in Applied microbiology and biotechnology Berlin : Springer, 1975 102(2017), 3 vom: 02. Dez., Seite 1281-1295 (DE-627)265509564 (DE-600)1464336-4 1432-0614 nnns volume:102 year:2017 number:3 day:02 month:12 pages:1281-1295 https://dx.doi.org/10.1007/s00253-017-8541-9 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2110 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2360 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 102 2017 3 02 12 1281-1295
language	English
source	Enthalten in Applied microbiology and biotechnology 102(2017), 3 vom: 02. Dez., Seite 1281-1295 volume:102 year:2017 number:3 day:02 month:12 pages:1281-1295
sourceStr	Enthalten in Applied microbiology and biotechnology 102(2017), 3 vom: 02. Dez., Seite 1281-1295 volume:102 year:2017 number:3 day:02 month:12 pages:1281-1295
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	β-Glucosidase Lignocellulose Lytic polysaccharide monooxygenase Plant biomass Reducing agent
isfreeaccess_bool	true
container_title	Applied microbiology and biotechnology
authorswithroles_txt_mv	Frommhagen, Matthias @@aut@@ Westphal, Adrie H. @@aut@@ Hilgers, Roelant @@aut@@ Koetsier, Martijn J. @@aut@@ Hinz, Sandra W. A. @@aut@@ Visser, Jaap @@aut@@ Gruppen, Harry @@aut@@ van Berkel, Willem J. H. @@aut@@ Kabel, Mirjam A. @@aut@@
publishDateDaySort_date	2017-12-02T00:00:00Z
hierarchy_top_id	265509564
id	SPR003030466
language_de	englisch
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author	Frommhagen, Matthias
spellingShingle	Frommhagen, Matthias misc β-Glucosidase misc Lignocellulose misc Lytic polysaccharide monooxygenase misc Plant biomass misc Reducing agent Quantification of the catalytic performance of C1-cellulose-specific lytic polysaccharide monooxygenases
authorStr	Frommhagen, Matthias
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topic_title	Quantification of the catalytic performance of C1-cellulose-specific lytic polysaccharide monooxygenases β-Glucosidase (dpeaa)DE-He213 Lignocellulose (dpeaa)DE-He213 Lytic polysaccharide monooxygenase (dpeaa)DE-He213 Plant biomass (dpeaa)DE-He213 Reducing agent (dpeaa)DE-He213
topic	misc β-Glucosidase misc Lignocellulose misc Lytic polysaccharide monooxygenase misc Plant biomass misc Reducing agent
topic_unstemmed	misc β-Glucosidase misc Lignocellulose misc Lytic polysaccharide monooxygenase misc Plant biomass misc Reducing agent
topic_browse	misc β-Glucosidase misc Lignocellulose misc Lytic polysaccharide monooxygenase misc Plant biomass misc Reducing agent
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title	Quantification of the catalytic performance of C1-cellulose-specific lytic polysaccharide monooxygenases
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title_full	Quantification of the catalytic performance of C1-cellulose-specific lytic polysaccharide monooxygenases
author_sort	Frommhagen, Matthias
journal	Applied microbiology and biotechnology
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author_browse	Frommhagen, Matthias Westphal, Adrie H. Hilgers, Roelant Koetsier, Martijn J. Hinz, Sandra W. A. Visser, Jaap Gruppen, Harry van Berkel, Willem J. H. Kabel, Mirjam A.
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author-letter	Frommhagen, Matthias
doi_str_mv	10.1007/s00253-017-8541-9
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title_sort	quantification of the catalytic performance of c1-cellulose-specific lytic polysaccharide monooxygenases
title_auth	Quantification of the catalytic performance of C1-cellulose-specific lytic polysaccharide monooxygenases
abstract	Abstract Lytic polysaccharide monooxygenases (LPMOs) have recently been shown to significantly enhance the degradation of recalcitrant polysaccharides and are of interest for the production of biochemicals and bioethanol from plant biomass. The copper-containing LPMOs utilize electrons, provided by reducing agents, to oxidatively cleave polysaccharides. Here, we report the development of a β-glucosidase-assisted method to quantify the release of C1-oxidized gluco-oligosaccharides from cellulose by two C1-oxidizing LPMOs from Myceliophthora thermophila C1. Based on this quantification method, we demonstrate that the catalytic performance of both MtLPMOs is strongly dependent on pH and temperature. The obtained results indicate that the catalytic performance of LPMOs depends on the interaction of multiple factors, which are affected by both pH and temperature. © The Author(s) 2017
abstractGer	Abstract Lytic polysaccharide monooxygenases (LPMOs) have recently been shown to significantly enhance the degradation of recalcitrant polysaccharides and are of interest for the production of biochemicals and bioethanol from plant biomass. The copper-containing LPMOs utilize electrons, provided by reducing agents, to oxidatively cleave polysaccharides. Here, we report the development of a β-glucosidase-assisted method to quantify the release of C1-oxidized gluco-oligosaccharides from cellulose by two C1-oxidizing LPMOs from Myceliophthora thermophila C1. Based on this quantification method, we demonstrate that the catalytic performance of both MtLPMOs is strongly dependent on pH and temperature. The obtained results indicate that the catalytic performance of LPMOs depends on the interaction of multiple factors, which are affected by both pH and temperature. © The Author(s) 2017
abstract_unstemmed	Abstract Lytic polysaccharide monooxygenases (LPMOs) have recently been shown to significantly enhance the degradation of recalcitrant polysaccharides and are of interest for the production of biochemicals and bioethanol from plant biomass. The copper-containing LPMOs utilize electrons, provided by reducing agents, to oxidatively cleave polysaccharides. Here, we report the development of a β-glucosidase-assisted method to quantify the release of C1-oxidized gluco-oligosaccharides from cellulose by two C1-oxidizing LPMOs from Myceliophthora thermophila C1. Based on this quantification method, we demonstrate that the catalytic performance of both MtLPMOs is strongly dependent on pH and temperature. The obtained results indicate that the catalytic performance of LPMOs depends on the interaction of multiple factors, which are affected by both pH and temperature. © The Author(s) 2017
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container_issue	3
title_short	Quantification of the catalytic performance of C1-cellulose-specific lytic polysaccharide monooxygenases
url	https://dx.doi.org/10.1007/s00253-017-8541-9
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author2	Westphal, Adrie H. Hilgers, Roelant Koetsier, Martijn J. Hinz, Sandra W. A. Visser, Jaap Gruppen, Harry van Berkel, Willem J. H. Kabel, Mirjam A.
author2Str	Westphal, Adrie H. Hilgers, Roelant Koetsier, Martijn J. Hinz, Sandra W. A. Visser, Jaap Gruppen, Harry van Berkel, Willem J. H. Kabel, Mirjam A.
ppnlink	265509564
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doi_str	10.1007/s00253-017-8541-9
up_date	2024-07-03T16:51:26.119Z
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Quantification of the catalytic performance of C1-cellulose-specific lytic polysaccharide monooxygenases

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