Processing Technique for Lipofilling Influences Adipose-Derived Stem Cell Concentration and Cell Viability in Lipoaspirate

Background Autologous fat grafting is a highly used technique in plastic and reconstructive surgery. Several fat-processing techniques have been described, with centrifugation frequently touted as the optimal method. Processing is one factor important for maximizing cell viability and adipose-derive...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Pfaff, Miles [verfasserIn] Wu, Wei Zellner, Elizabeth Steinbacher, Derek M.

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2014

Schlagwörter:	Telfa-rolling Centrifugation Fat grafting Fat processing Lipoaspirate Lipoaspiration Adipose-derived mesenchymal stem cells Adipose-derived stem cells ADSC Lipofilling

Anmerkung:	© Springer Science+Business Media New York and International Society of Aesthetic Plastic Surgery 2014

Übergeordnetes Werk:	Enthalten in: Aesthetic plastic surgery - New York, NY : Springer, 1976, 38(2014), 1 vom: 08. Jan., Seite 224-229
Übergeordnetes Werk:	volume:38 ; year:2014 ; number:1 ; day:08 ; month:01 ; pages:224-229

Links:	Volltext

DOI / URN:	10.1007/s00266-013-0261-7

Katalog-ID:	SPR003347508

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520			\|a Background Autologous fat grafting is a highly used technique in plastic and reconstructive surgery. Several fat-processing techniques have been described, with centrifugation frequently touted as the optimal method. Processing is one factor important for maximizing cell viability and adipose-derived mesenchymal stem cell (ADSC) concentrations. This study compared two methods of fat preparation (centrifugation vs Telfa-rolling) to determine which technique results in the greatest degree of cell viability and ADSC concentration. Methods Abdominal fat was harvested from five patients. Equal aliquots were divided and processed by both centrifugation and Telfa-rolling. Samples were analyzed for ADSC proportions via flow cytometry and cell viability using methylene blue-based cell counting. Paired t tests were performed on all samples, and a P value lower than 0.05 was considered statistically significant. Results Telfa-rolling processing resulted in a higher percentage of isolated ADSCs (P < 0.5 for 3 of 4 parameters) and a significantly higher number of viable cells (P < 0.05). Conclusion Telfa-rolling results in a higher proportion of ADSCs and greater cell viability than centrifugation for donor adipose graft preparation. Further studies are necessary to confirm whether optimal preparation translates to improved augmentation and cell take at the recipient site. Level of Evidence IV This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266.
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650		4	\|a Lipoaspirate \|7 (dpeaa)DE-He213
650		4	\|a Lipoaspiration \|7 (dpeaa)DE-He213
650		4	\|a Adipose-derived mesenchymal stem cells \|7 (dpeaa)DE-He213
650		4	\|a Adipose-derived stem cells \|7 (dpeaa)DE-He213
650		4	\|a ADSC \|7 (dpeaa)DE-He213
650		4	\|a Lipofilling \|7 (dpeaa)DE-He213
700	1		\|a Wu, Wei \|4 aut
700	1		\|a Zellner, Elizabeth \|4 aut
700	1		\|a Steinbacher, Derek M. \|4 aut
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allfields	10.1007/s00266-013-0261-7 doi (DE-627)SPR003347508 (SPR)s00266-013-0261-7-e DE-627 ger DE-627 rakwb eng Pfaff, Miles verfasserin aut Processing Technique for Lipofilling Influences Adipose-Derived Stem Cell Concentration and Cell Viability in Lipoaspirate 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer Science+Business Media New York and International Society of Aesthetic Plastic Surgery 2014 Background Autologous fat grafting is a highly used technique in plastic and reconstructive surgery. Several fat-processing techniques have been described, with centrifugation frequently touted as the optimal method. Processing is one factor important for maximizing cell viability and adipose-derived mesenchymal stem cell (ADSC) concentrations. This study compared two methods of fat preparation (centrifugation vs Telfa-rolling) to determine which technique results in the greatest degree of cell viability and ADSC concentration. Methods Abdominal fat was harvested from five patients. Equal aliquots were divided and processed by both centrifugation and Telfa-rolling. Samples were analyzed for ADSC proportions via flow cytometry and cell viability using methylene blue-based cell counting. Paired t tests were performed on all samples, and a P value lower than 0.05 was considered statistically significant. Results Telfa-rolling processing resulted in a higher percentage of isolated ADSCs (P < 0.5 for 3 of 4 parameters) and a significantly higher number of viable cells (P < 0.05). Conclusion Telfa-rolling results in a higher proportion of ADSCs and greater cell viability than centrifugation for donor adipose graft preparation. Further studies are necessary to confirm whether optimal preparation translates to improved augmentation and cell take at the recipient site. Level of Evidence IV This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266. Telfa-rolling (dpeaa)DE-He213 Centrifugation (dpeaa)DE-He213 Fat grafting (dpeaa)DE-He213 Fat processing (dpeaa)DE-He213 Lipoaspirate (dpeaa)DE-He213 Lipoaspiration (dpeaa)DE-He213 Adipose-derived mesenchymal stem cells (dpeaa)DE-He213 Adipose-derived stem cells (dpeaa)DE-He213 ADSC (dpeaa)DE-He213 Lipofilling (dpeaa)DE-He213 Wu, Wei aut Zellner, Elizabeth aut Steinbacher, Derek M. aut Enthalten in Aesthetic plastic surgery New York, NY : Springer, 1976 38(2014), 1 vom: 08. Jan., Seite 224-229 (DE-627)254630758 (DE-600)1462126-5 1432-5241 nnns volume:38 year:2014 number:1 day:08 month:01 pages:224-229 https://dx.doi.org/10.1007/s00266-013-0261-7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_711 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 38 2014 1 08 01 224-229
spelling	10.1007/s00266-013-0261-7 doi (DE-627)SPR003347508 (SPR)s00266-013-0261-7-e DE-627 ger DE-627 rakwb eng Pfaff, Miles verfasserin aut Processing Technique for Lipofilling Influences Adipose-Derived Stem Cell Concentration and Cell Viability in Lipoaspirate 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer Science+Business Media New York and International Society of Aesthetic Plastic Surgery 2014 Background Autologous fat grafting is a highly used technique in plastic and reconstructive surgery. Several fat-processing techniques have been described, with centrifugation frequently touted as the optimal method. Processing is one factor important for maximizing cell viability and adipose-derived mesenchymal stem cell (ADSC) concentrations. This study compared two methods of fat preparation (centrifugation vs Telfa-rolling) to determine which technique results in the greatest degree of cell viability and ADSC concentration. Methods Abdominal fat was harvested from five patients. Equal aliquots were divided and processed by both centrifugation and Telfa-rolling. Samples were analyzed for ADSC proportions via flow cytometry and cell viability using methylene blue-based cell counting. Paired t tests were performed on all samples, and a P value lower than 0.05 was considered statistically significant. Results Telfa-rolling processing resulted in a higher percentage of isolated ADSCs (P < 0.5 for 3 of 4 parameters) and a significantly higher number of viable cells (P < 0.05). Conclusion Telfa-rolling results in a higher proportion of ADSCs and greater cell viability than centrifugation for donor adipose graft preparation. Further studies are necessary to confirm whether optimal preparation translates to improved augmentation and cell take at the recipient site. Level of Evidence IV This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266. Telfa-rolling (dpeaa)DE-He213 Centrifugation (dpeaa)DE-He213 Fat grafting (dpeaa)DE-He213 Fat processing (dpeaa)DE-He213 Lipoaspirate (dpeaa)DE-He213 Lipoaspiration (dpeaa)DE-He213 Adipose-derived mesenchymal stem cells (dpeaa)DE-He213 Adipose-derived stem cells (dpeaa)DE-He213 ADSC (dpeaa)DE-He213 Lipofilling (dpeaa)DE-He213 Wu, Wei aut Zellner, Elizabeth aut Steinbacher, Derek M. aut Enthalten in Aesthetic plastic surgery New York, NY : Springer, 1976 38(2014), 1 vom: 08. Jan., Seite 224-229 (DE-627)254630758 (DE-600)1462126-5 1432-5241 nnns volume:38 year:2014 number:1 day:08 month:01 pages:224-229 https://dx.doi.org/10.1007/s00266-013-0261-7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_711 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 38 2014 1 08 01 224-229
allfields_unstemmed	10.1007/s00266-013-0261-7 doi (DE-627)SPR003347508 (SPR)s00266-013-0261-7-e DE-627 ger DE-627 rakwb eng Pfaff, Miles verfasserin aut Processing Technique for Lipofilling Influences Adipose-Derived Stem Cell Concentration and Cell Viability in Lipoaspirate 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer Science+Business Media New York and International Society of Aesthetic Plastic Surgery 2014 Background Autologous fat grafting is a highly used technique in plastic and reconstructive surgery. Several fat-processing techniques have been described, with centrifugation frequently touted as the optimal method. Processing is one factor important for maximizing cell viability and adipose-derived mesenchymal stem cell (ADSC) concentrations. This study compared two methods of fat preparation (centrifugation vs Telfa-rolling) to determine which technique results in the greatest degree of cell viability and ADSC concentration. Methods Abdominal fat was harvested from five patients. Equal aliquots were divided and processed by both centrifugation and Telfa-rolling. Samples were analyzed for ADSC proportions via flow cytometry and cell viability using methylene blue-based cell counting. Paired t tests were performed on all samples, and a P value lower than 0.05 was considered statistically significant. Results Telfa-rolling processing resulted in a higher percentage of isolated ADSCs (P < 0.5 for 3 of 4 parameters) and a significantly higher number of viable cells (P < 0.05). Conclusion Telfa-rolling results in a higher proportion of ADSCs and greater cell viability than centrifugation for donor adipose graft preparation. Further studies are necessary to confirm whether optimal preparation translates to improved augmentation and cell take at the recipient site. Level of Evidence IV This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266. Telfa-rolling (dpeaa)DE-He213 Centrifugation (dpeaa)DE-He213 Fat grafting (dpeaa)DE-He213 Fat processing (dpeaa)DE-He213 Lipoaspirate (dpeaa)DE-He213 Lipoaspiration (dpeaa)DE-He213 Adipose-derived mesenchymal stem cells (dpeaa)DE-He213 Adipose-derived stem cells (dpeaa)DE-He213 ADSC (dpeaa)DE-He213 Lipofilling (dpeaa)DE-He213 Wu, Wei aut Zellner, Elizabeth aut Steinbacher, Derek M. aut Enthalten in Aesthetic plastic surgery New York, NY : Springer, 1976 38(2014), 1 vom: 08. Jan., Seite 224-229 (DE-627)254630758 (DE-600)1462126-5 1432-5241 nnns volume:38 year:2014 number:1 day:08 month:01 pages:224-229 https://dx.doi.org/10.1007/s00266-013-0261-7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_711 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 38 2014 1 08 01 224-229
allfieldsGer	10.1007/s00266-013-0261-7 doi (DE-627)SPR003347508 (SPR)s00266-013-0261-7-e DE-627 ger DE-627 rakwb eng Pfaff, Miles verfasserin aut Processing Technique for Lipofilling Influences Adipose-Derived Stem Cell Concentration and Cell Viability in Lipoaspirate 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer Science+Business Media New York and International Society of Aesthetic Plastic Surgery 2014 Background Autologous fat grafting is a highly used technique in plastic and reconstructive surgery. Several fat-processing techniques have been described, with centrifugation frequently touted as the optimal method. Processing is one factor important for maximizing cell viability and adipose-derived mesenchymal stem cell (ADSC) concentrations. This study compared two methods of fat preparation (centrifugation vs Telfa-rolling) to determine which technique results in the greatest degree of cell viability and ADSC concentration. Methods Abdominal fat was harvested from five patients. Equal aliquots were divided and processed by both centrifugation and Telfa-rolling. Samples were analyzed for ADSC proportions via flow cytometry and cell viability using methylene blue-based cell counting. Paired t tests were performed on all samples, and a P value lower than 0.05 was considered statistically significant. Results Telfa-rolling processing resulted in a higher percentage of isolated ADSCs (P < 0.5 for 3 of 4 parameters) and a significantly higher number of viable cells (P < 0.05). Conclusion Telfa-rolling results in a higher proportion of ADSCs and greater cell viability than centrifugation for donor adipose graft preparation. Further studies are necessary to confirm whether optimal preparation translates to improved augmentation and cell take at the recipient site. Level of Evidence IV This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266. Telfa-rolling (dpeaa)DE-He213 Centrifugation (dpeaa)DE-He213 Fat grafting (dpeaa)DE-He213 Fat processing (dpeaa)DE-He213 Lipoaspirate (dpeaa)DE-He213 Lipoaspiration (dpeaa)DE-He213 Adipose-derived mesenchymal stem cells (dpeaa)DE-He213 Adipose-derived stem cells (dpeaa)DE-He213 ADSC (dpeaa)DE-He213 Lipofilling (dpeaa)DE-He213 Wu, Wei aut Zellner, Elizabeth aut Steinbacher, Derek M. aut Enthalten in Aesthetic plastic surgery New York, NY : Springer, 1976 38(2014), 1 vom: 08. Jan., Seite 224-229 (DE-627)254630758 (DE-600)1462126-5 1432-5241 nnns volume:38 year:2014 number:1 day:08 month:01 pages:224-229 https://dx.doi.org/10.1007/s00266-013-0261-7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_711 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 38 2014 1 08 01 224-229
allfieldsSound	10.1007/s00266-013-0261-7 doi (DE-627)SPR003347508 (SPR)s00266-013-0261-7-e DE-627 ger DE-627 rakwb eng Pfaff, Miles verfasserin aut Processing Technique for Lipofilling Influences Adipose-Derived Stem Cell Concentration and Cell Viability in Lipoaspirate 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer Science+Business Media New York and International Society of Aesthetic Plastic Surgery 2014 Background Autologous fat grafting is a highly used technique in plastic and reconstructive surgery. Several fat-processing techniques have been described, with centrifugation frequently touted as the optimal method. Processing is one factor important for maximizing cell viability and adipose-derived mesenchymal stem cell (ADSC) concentrations. This study compared two methods of fat preparation (centrifugation vs Telfa-rolling) to determine which technique results in the greatest degree of cell viability and ADSC concentration. Methods Abdominal fat was harvested from five patients. Equal aliquots were divided and processed by both centrifugation and Telfa-rolling. Samples were analyzed for ADSC proportions via flow cytometry and cell viability using methylene blue-based cell counting. Paired t tests were performed on all samples, and a P value lower than 0.05 was considered statistically significant. Results Telfa-rolling processing resulted in a higher percentage of isolated ADSCs (P < 0.5 for 3 of 4 parameters) and a significantly higher number of viable cells (P < 0.05). Conclusion Telfa-rolling results in a higher proportion of ADSCs and greater cell viability than centrifugation for donor adipose graft preparation. Further studies are necessary to confirm whether optimal preparation translates to improved augmentation and cell take at the recipient site. Level of Evidence IV This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266. Telfa-rolling (dpeaa)DE-He213 Centrifugation (dpeaa)DE-He213 Fat grafting (dpeaa)DE-He213 Fat processing (dpeaa)DE-He213 Lipoaspirate (dpeaa)DE-He213 Lipoaspiration (dpeaa)DE-He213 Adipose-derived mesenchymal stem cells (dpeaa)DE-He213 Adipose-derived stem cells (dpeaa)DE-He213 ADSC (dpeaa)DE-He213 Lipofilling (dpeaa)DE-He213 Wu, Wei aut Zellner, Elizabeth aut Steinbacher, Derek M. aut Enthalten in Aesthetic plastic surgery New York, NY : Springer, 1976 38(2014), 1 vom: 08. Jan., Seite 224-229 (DE-627)254630758 (DE-600)1462126-5 1432-5241 nnns volume:38 year:2014 number:1 day:08 month:01 pages:224-229 https://dx.doi.org/10.1007/s00266-013-0261-7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_711 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 38 2014 1 08 01 224-229
language	English
source	Enthalten in Aesthetic plastic surgery 38(2014), 1 vom: 08. Jan., Seite 224-229 volume:38 year:2014 number:1 day:08 month:01 pages:224-229
sourceStr	Enthalten in Aesthetic plastic surgery 38(2014), 1 vom: 08. Jan., Seite 224-229 volume:38 year:2014 number:1 day:08 month:01 pages:224-229
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Telfa-rolling Centrifugation Fat grafting Fat processing Lipoaspirate Lipoaspiration Adipose-derived mesenchymal stem cells Adipose-derived stem cells ADSC Lipofilling
isfreeaccess_bool	false
container_title	Aesthetic plastic surgery
authorswithroles_txt_mv	Pfaff, Miles @@aut@@ Wu, Wei @@aut@@ Zellner, Elizabeth @@aut@@ Steinbacher, Derek M. @@aut@@
publishDateDaySort_date	2014-01-08T00:00:00Z
hierarchy_top_id	254630758
id	SPR003347508
language_de	englisch
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author	Pfaff, Miles
spellingShingle	Pfaff, Miles misc Telfa-rolling misc Centrifugation misc Fat grafting misc Fat processing misc Lipoaspirate misc Lipoaspiration misc Adipose-derived mesenchymal stem cells misc Adipose-derived stem cells misc ADSC misc Lipofilling Processing Technique for Lipofilling Influences Adipose-Derived Stem Cell Concentration and Cell Viability in Lipoaspirate
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topic_title	Processing Technique for Lipofilling Influences Adipose-Derived Stem Cell Concentration and Cell Viability in Lipoaspirate Telfa-rolling (dpeaa)DE-He213 Centrifugation (dpeaa)DE-He213 Fat grafting (dpeaa)DE-He213 Fat processing (dpeaa)DE-He213 Lipoaspirate (dpeaa)DE-He213 Lipoaspiration (dpeaa)DE-He213 Adipose-derived mesenchymal stem cells (dpeaa)DE-He213 Adipose-derived stem cells (dpeaa)DE-He213 ADSC (dpeaa)DE-He213 Lipofilling (dpeaa)DE-He213
topic	misc Telfa-rolling misc Centrifugation misc Fat grafting misc Fat processing misc Lipoaspirate misc Lipoaspiration misc Adipose-derived mesenchymal stem cells misc Adipose-derived stem cells misc ADSC misc Lipofilling
topic_unstemmed	misc Telfa-rolling misc Centrifugation misc Fat grafting misc Fat processing misc Lipoaspirate misc Lipoaspiration misc Adipose-derived mesenchymal stem cells misc Adipose-derived stem cells misc ADSC misc Lipofilling
topic_browse	misc Telfa-rolling misc Centrifugation misc Fat grafting misc Fat processing misc Lipoaspirate misc Lipoaspiration misc Adipose-derived mesenchymal stem cells misc Adipose-derived stem cells misc ADSC misc Lipofilling
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title	Processing Technique for Lipofilling Influences Adipose-Derived Stem Cell Concentration and Cell Viability in Lipoaspirate
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title_full	Processing Technique for Lipofilling Influences Adipose-Derived Stem Cell Concentration and Cell Viability in Lipoaspirate
author_sort	Pfaff, Miles
journal	Aesthetic plastic surgery
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container_start_page	224
author_browse	Pfaff, Miles Wu, Wei Zellner, Elizabeth Steinbacher, Derek M.
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author-letter	Pfaff, Miles
doi_str_mv	10.1007/s00266-013-0261-7
title_sort	processing technique for lipofilling influences adipose-derived stem cell concentration and cell viability in lipoaspirate
title_auth	Processing Technique for Lipofilling Influences Adipose-Derived Stem Cell Concentration and Cell Viability in Lipoaspirate
abstract	Background Autologous fat grafting is a highly used technique in plastic and reconstructive surgery. Several fat-processing techniques have been described, with centrifugation frequently touted as the optimal method. Processing is one factor important for maximizing cell viability and adipose-derived mesenchymal stem cell (ADSC) concentrations. This study compared two methods of fat preparation (centrifugation vs Telfa-rolling) to determine which technique results in the greatest degree of cell viability and ADSC concentration. Methods Abdominal fat was harvested from five patients. Equal aliquots were divided and processed by both centrifugation and Telfa-rolling. Samples were analyzed for ADSC proportions via flow cytometry and cell viability using methylene blue-based cell counting. Paired t tests were performed on all samples, and a P value lower than 0.05 was considered statistically significant. Results Telfa-rolling processing resulted in a higher percentage of isolated ADSCs (P < 0.5 for 3 of 4 parameters) and a significantly higher number of viable cells (P < 0.05). Conclusion Telfa-rolling results in a higher proportion of ADSCs and greater cell viability than centrifugation for donor adipose graft preparation. Further studies are necessary to confirm whether optimal preparation translates to improved augmentation and cell take at the recipient site. Level of Evidence IV This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266. © Springer Science+Business Media New York and International Society of Aesthetic Plastic Surgery 2014
abstractGer	Background Autologous fat grafting is a highly used technique in plastic and reconstructive surgery. Several fat-processing techniques have been described, with centrifugation frequently touted as the optimal method. Processing is one factor important for maximizing cell viability and adipose-derived mesenchymal stem cell (ADSC) concentrations. This study compared two methods of fat preparation (centrifugation vs Telfa-rolling) to determine which technique results in the greatest degree of cell viability and ADSC concentration. Methods Abdominal fat was harvested from five patients. Equal aliquots were divided and processed by both centrifugation and Telfa-rolling. Samples were analyzed for ADSC proportions via flow cytometry and cell viability using methylene blue-based cell counting. Paired t tests were performed on all samples, and a P value lower than 0.05 was considered statistically significant. Results Telfa-rolling processing resulted in a higher percentage of isolated ADSCs (P < 0.5 for 3 of 4 parameters) and a significantly higher number of viable cells (P < 0.05). Conclusion Telfa-rolling results in a higher proportion of ADSCs and greater cell viability than centrifugation for donor adipose graft preparation. Further studies are necessary to confirm whether optimal preparation translates to improved augmentation and cell take at the recipient site. Level of Evidence IV This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266. © Springer Science+Business Media New York and International Society of Aesthetic Plastic Surgery 2014
abstract_unstemmed	Background Autologous fat grafting is a highly used technique in plastic and reconstructive surgery. Several fat-processing techniques have been described, with centrifugation frequently touted as the optimal method. Processing is one factor important for maximizing cell viability and adipose-derived mesenchymal stem cell (ADSC) concentrations. This study compared two methods of fat preparation (centrifugation vs Telfa-rolling) to determine which technique results in the greatest degree of cell viability and ADSC concentration. Methods Abdominal fat was harvested from five patients. Equal aliquots were divided and processed by both centrifugation and Telfa-rolling. Samples were analyzed for ADSC proportions via flow cytometry and cell viability using methylene blue-based cell counting. Paired t tests were performed on all samples, and a P value lower than 0.05 was considered statistically significant. Results Telfa-rolling processing resulted in a higher percentage of isolated ADSCs (P < 0.5 for 3 of 4 parameters) and a significantly higher number of viable cells (P < 0.05). Conclusion Telfa-rolling results in a higher proportion of ADSCs and greater cell viability than centrifugation for donor adipose graft preparation. Further studies are necessary to confirm whether optimal preparation translates to improved augmentation and cell take at the recipient site. Level of Evidence IV This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266. © Springer Science+Business Media New York and International Society of Aesthetic Plastic Surgery 2014
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title_short	Processing Technique for Lipofilling Influences Adipose-Derived Stem Cell Concentration and Cell Viability in Lipoaspirate
url	https://dx.doi.org/10.1007/s00266-013-0261-7
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author2	Wu, Wei Zellner, Elizabeth Steinbacher, Derek M.
author2Str	Wu, Wei Zellner, Elizabeth Steinbacher, Derek M.
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doi_str	10.1007/s00266-013-0261-7
up_date	2024-07-03T18:58:10.146Z
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Processing Technique for Lipofilling Influences Adipose-Derived Stem Cell Concentration and Cell Viability in Lipoaspirate

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