Microarray Analysis of Somatostatin Receptor 5-Regulated Gene Expression Profiles in Murine Pancreas
Background We previously demonstrated that somatostatin receptor type 5 (SSTR5) gene ablation results in alterations in insulin secretion and glucose metabolism, accompanied by morphologic alterations in the islets of Langerhans. The underlying mechanism(s) by which SSTR5 exerts its cellular functio...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Patel, Sanjeet G. [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2009 |
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| Schlagwörter: |
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| Anmerkung: |
© Société Internationale de Chirurgie 2009 |
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| Übergeordnetes Werk: |
Enthalten in: World Journal of Surgery - Springer-Verlag, 1996, 33(2009), 4 vom: 10. Jan. |
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| Übergeordnetes Werk: |
volume:33 ; year:2009 ; number:4 ; day:10 ; month:01 |
| Links: |
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| DOI / URN: |
10.1007/s00268-008-9893-1 |
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SPR003422259 |
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| 100 | 1 | |a Patel, Sanjeet G. |e verfasserin |4 aut | |
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| 520 | |a Background We previously demonstrated that somatostatin receptor type 5 (SSTR5) gene ablation results in alterations in insulin secretion and glucose metabolism, accompanied by morphologic alterations in the islets of Langerhans. The underlying mechanism(s) by which SSTR5 exerts its cellular functions remain(s) unknown. We hypothesized that SSTR5 mediates the inhibitory effect of somatostatin (SST) on insulin secretion and islet proliferation by regulating a specific set of pancreatic genes. Methods To identify SSTR5-regulated pancreatic genes, gene expression microarray analysis was performed on the whole pancreas of 1- and 3-month-old wild-type (WT) and SSTR5 knockout (SSTR5−/−) male mice. Real-time RT-PCR and immunofluorescence were performed to validate selected differentially expressed genes. Results A set of 143 probes were identified to be differentially expressed in the pancreas of 1-month-old SSTR5−/− mice, 72 of which were downregulated and 71 upregulated. At 3 months of age, SSTR5 gene ablation resulted in downregulation of a set of 30 probes and upregulation of a set of 37 probes. Among these differentially expressed genes, there were 15 and 5 genes that were upregulated and downregulated, respectively, in mice at both 1 and 3 months of age. Three genes, PAP/INGAP, ANG, and TDE1, were selected to be validated by real-time RT-PCR and immunofluorescence. Conclusions A specific set of genes linked to a wide range of cellular functions such as islet proliferation, apoptosis, angiogenesis, and tumorigenesis were either upregulated or downregulated in SSTR5-deficient male mice compared with their expression in wild-type mice. Therefore, these genes are potential SSTR5-regulated genes during normal pancreatic development and functional maintenance. | ||
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10.1007/s00268-008-9893-1 doi (DE-627)SPR003422259 (SPR)s00268-008-9893-1-e DE-627 ger DE-627 rakwb eng Patel, Sanjeet G. verfasserin aut Microarray Analysis of Somatostatin Receptor 5-Regulated Gene Expression Profiles in Murine Pancreas 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Société Internationale de Chirurgie 2009 Background We previously demonstrated that somatostatin receptor type 5 (SSTR5) gene ablation results in alterations in insulin secretion and glucose metabolism, accompanied by morphologic alterations in the islets of Langerhans. The underlying mechanism(s) by which SSTR5 exerts its cellular functions remain(s) unknown. We hypothesized that SSTR5 mediates the inhibitory effect of somatostatin (SST) on insulin secretion and islet proliferation by regulating a specific set of pancreatic genes. Methods To identify SSTR5-regulated pancreatic genes, gene expression microarray analysis was performed on the whole pancreas of 1- and 3-month-old wild-type (WT) and SSTR5 knockout (SSTR5−/−) male mice. Real-time RT-PCR and immunofluorescence were performed to validate selected differentially expressed genes. Results A set of 143 probes were identified to be differentially expressed in the pancreas of 1-month-old SSTR5−/− mice, 72 of which were downregulated and 71 upregulated. At 3 months of age, SSTR5 gene ablation resulted in downregulation of a set of 30 probes and upregulation of a set of 37 probes. Among these differentially expressed genes, there were 15 and 5 genes that were upregulated and downregulated, respectively, in mice at both 1 and 3 months of age. Three genes, PAP/INGAP, ANG, and TDE1, were selected to be validated by real-time RT-PCR and immunofluorescence. Conclusions A specific set of genes linked to a wide range of cellular functions such as islet proliferation, apoptosis, angiogenesis, and tumorigenesis were either upregulated or downregulated in SSTR5-deficient male mice compared with their expression in wild-type mice. Therefore, these genes are potential SSTR5-regulated genes during normal pancreatic development and functional maintenance. ANG1 Expression (dpeaa)DE-He213 Gene Expression Alteration (dpeaa)DE-He213 Islet Neogenesis (dpeaa)DE-He213 Islet Hyperplasia (dpeaa)DE-He213 Islet Cell Proliferation (dpeaa)DE-He213 Zhou, Guisheng aut Liu, Shi-He aut Li, Min aut Jeong, Jae-Wook aut DeMayo, Francesco J. aut Gingras, Marie-Claude aut Gibbs, Richard A. aut Fisher, William E. aut Brunicardi, F. Charles aut Enthalten in World Journal of Surgery Springer-Verlag, 1996 33(2009), 4 vom: 10. Jan. (DE-627)SPR003391159 nnns volume:33 year:2009 number:4 day:10 month:01 https://dx.doi.org/10.1007/s00268-008-9893-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 33 2009 4 10 01 |
| spelling |
10.1007/s00268-008-9893-1 doi (DE-627)SPR003422259 (SPR)s00268-008-9893-1-e DE-627 ger DE-627 rakwb eng Patel, Sanjeet G. verfasserin aut Microarray Analysis of Somatostatin Receptor 5-Regulated Gene Expression Profiles in Murine Pancreas 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Société Internationale de Chirurgie 2009 Background We previously demonstrated that somatostatin receptor type 5 (SSTR5) gene ablation results in alterations in insulin secretion and glucose metabolism, accompanied by morphologic alterations in the islets of Langerhans. The underlying mechanism(s) by which SSTR5 exerts its cellular functions remain(s) unknown. We hypothesized that SSTR5 mediates the inhibitory effect of somatostatin (SST) on insulin secretion and islet proliferation by regulating a specific set of pancreatic genes. Methods To identify SSTR5-regulated pancreatic genes, gene expression microarray analysis was performed on the whole pancreas of 1- and 3-month-old wild-type (WT) and SSTR5 knockout (SSTR5−/−) male mice. Real-time RT-PCR and immunofluorescence were performed to validate selected differentially expressed genes. Results A set of 143 probes were identified to be differentially expressed in the pancreas of 1-month-old SSTR5−/− mice, 72 of which were downregulated and 71 upregulated. At 3 months of age, SSTR5 gene ablation resulted in downregulation of a set of 30 probes and upregulation of a set of 37 probes. Among these differentially expressed genes, there were 15 and 5 genes that were upregulated and downregulated, respectively, in mice at both 1 and 3 months of age. Three genes, PAP/INGAP, ANG, and TDE1, were selected to be validated by real-time RT-PCR and immunofluorescence. Conclusions A specific set of genes linked to a wide range of cellular functions such as islet proliferation, apoptosis, angiogenesis, and tumorigenesis were either upregulated or downregulated in SSTR5-deficient male mice compared with their expression in wild-type mice. Therefore, these genes are potential SSTR5-regulated genes during normal pancreatic development and functional maintenance. ANG1 Expression (dpeaa)DE-He213 Gene Expression Alteration (dpeaa)DE-He213 Islet Neogenesis (dpeaa)DE-He213 Islet Hyperplasia (dpeaa)DE-He213 Islet Cell Proliferation (dpeaa)DE-He213 Zhou, Guisheng aut Liu, Shi-He aut Li, Min aut Jeong, Jae-Wook aut DeMayo, Francesco J. aut Gingras, Marie-Claude aut Gibbs, Richard A. aut Fisher, William E. aut Brunicardi, F. Charles aut Enthalten in World Journal of Surgery Springer-Verlag, 1996 33(2009), 4 vom: 10. Jan. (DE-627)SPR003391159 nnns volume:33 year:2009 number:4 day:10 month:01 https://dx.doi.org/10.1007/s00268-008-9893-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 33 2009 4 10 01 |
| allfields_unstemmed |
10.1007/s00268-008-9893-1 doi (DE-627)SPR003422259 (SPR)s00268-008-9893-1-e DE-627 ger DE-627 rakwb eng Patel, Sanjeet G. verfasserin aut Microarray Analysis of Somatostatin Receptor 5-Regulated Gene Expression Profiles in Murine Pancreas 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Société Internationale de Chirurgie 2009 Background We previously demonstrated that somatostatin receptor type 5 (SSTR5) gene ablation results in alterations in insulin secretion and glucose metabolism, accompanied by morphologic alterations in the islets of Langerhans. The underlying mechanism(s) by which SSTR5 exerts its cellular functions remain(s) unknown. We hypothesized that SSTR5 mediates the inhibitory effect of somatostatin (SST) on insulin secretion and islet proliferation by regulating a specific set of pancreatic genes. Methods To identify SSTR5-regulated pancreatic genes, gene expression microarray analysis was performed on the whole pancreas of 1- and 3-month-old wild-type (WT) and SSTR5 knockout (SSTR5−/−) male mice. Real-time RT-PCR and immunofluorescence were performed to validate selected differentially expressed genes. Results A set of 143 probes were identified to be differentially expressed in the pancreas of 1-month-old SSTR5−/− mice, 72 of which were downregulated and 71 upregulated. At 3 months of age, SSTR5 gene ablation resulted in downregulation of a set of 30 probes and upregulation of a set of 37 probes. Among these differentially expressed genes, there were 15 and 5 genes that were upregulated and downregulated, respectively, in mice at both 1 and 3 months of age. Three genes, PAP/INGAP, ANG, and TDE1, were selected to be validated by real-time RT-PCR and immunofluorescence. Conclusions A specific set of genes linked to a wide range of cellular functions such as islet proliferation, apoptosis, angiogenesis, and tumorigenesis were either upregulated or downregulated in SSTR5-deficient male mice compared with their expression in wild-type mice. Therefore, these genes are potential SSTR5-regulated genes during normal pancreatic development and functional maintenance. ANG1 Expression (dpeaa)DE-He213 Gene Expression Alteration (dpeaa)DE-He213 Islet Neogenesis (dpeaa)DE-He213 Islet Hyperplasia (dpeaa)DE-He213 Islet Cell Proliferation (dpeaa)DE-He213 Zhou, Guisheng aut Liu, Shi-He aut Li, Min aut Jeong, Jae-Wook aut DeMayo, Francesco J. aut Gingras, Marie-Claude aut Gibbs, Richard A. aut Fisher, William E. aut Brunicardi, F. Charles aut Enthalten in World Journal of Surgery Springer-Verlag, 1996 33(2009), 4 vom: 10. Jan. (DE-627)SPR003391159 nnns volume:33 year:2009 number:4 day:10 month:01 https://dx.doi.org/10.1007/s00268-008-9893-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 33 2009 4 10 01 |
| allfieldsGer |
10.1007/s00268-008-9893-1 doi (DE-627)SPR003422259 (SPR)s00268-008-9893-1-e DE-627 ger DE-627 rakwb eng Patel, Sanjeet G. verfasserin aut Microarray Analysis of Somatostatin Receptor 5-Regulated Gene Expression Profiles in Murine Pancreas 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Société Internationale de Chirurgie 2009 Background We previously demonstrated that somatostatin receptor type 5 (SSTR5) gene ablation results in alterations in insulin secretion and glucose metabolism, accompanied by morphologic alterations in the islets of Langerhans. The underlying mechanism(s) by which SSTR5 exerts its cellular functions remain(s) unknown. We hypothesized that SSTR5 mediates the inhibitory effect of somatostatin (SST) on insulin secretion and islet proliferation by regulating a specific set of pancreatic genes. Methods To identify SSTR5-regulated pancreatic genes, gene expression microarray analysis was performed on the whole pancreas of 1- and 3-month-old wild-type (WT) and SSTR5 knockout (SSTR5−/−) male mice. Real-time RT-PCR and immunofluorescence were performed to validate selected differentially expressed genes. Results A set of 143 probes were identified to be differentially expressed in the pancreas of 1-month-old SSTR5−/− mice, 72 of which were downregulated and 71 upregulated. At 3 months of age, SSTR5 gene ablation resulted in downregulation of a set of 30 probes and upregulation of a set of 37 probes. Among these differentially expressed genes, there were 15 and 5 genes that were upregulated and downregulated, respectively, in mice at both 1 and 3 months of age. Three genes, PAP/INGAP, ANG, and TDE1, were selected to be validated by real-time RT-PCR and immunofluorescence. Conclusions A specific set of genes linked to a wide range of cellular functions such as islet proliferation, apoptosis, angiogenesis, and tumorigenesis were either upregulated or downregulated in SSTR5-deficient male mice compared with their expression in wild-type mice. Therefore, these genes are potential SSTR5-regulated genes during normal pancreatic development and functional maintenance. ANG1 Expression (dpeaa)DE-He213 Gene Expression Alteration (dpeaa)DE-He213 Islet Neogenesis (dpeaa)DE-He213 Islet Hyperplasia (dpeaa)DE-He213 Islet Cell Proliferation (dpeaa)DE-He213 Zhou, Guisheng aut Liu, Shi-He aut Li, Min aut Jeong, Jae-Wook aut DeMayo, Francesco J. aut Gingras, Marie-Claude aut Gibbs, Richard A. aut Fisher, William E. aut Brunicardi, F. Charles aut Enthalten in World Journal of Surgery Springer-Verlag, 1996 33(2009), 4 vom: 10. Jan. (DE-627)SPR003391159 nnns volume:33 year:2009 number:4 day:10 month:01 https://dx.doi.org/10.1007/s00268-008-9893-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 33 2009 4 10 01 |
| allfieldsSound |
10.1007/s00268-008-9893-1 doi (DE-627)SPR003422259 (SPR)s00268-008-9893-1-e DE-627 ger DE-627 rakwb eng Patel, Sanjeet G. verfasserin aut Microarray Analysis of Somatostatin Receptor 5-Regulated Gene Expression Profiles in Murine Pancreas 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Société Internationale de Chirurgie 2009 Background We previously demonstrated that somatostatin receptor type 5 (SSTR5) gene ablation results in alterations in insulin secretion and glucose metabolism, accompanied by morphologic alterations in the islets of Langerhans. The underlying mechanism(s) by which SSTR5 exerts its cellular functions remain(s) unknown. We hypothesized that SSTR5 mediates the inhibitory effect of somatostatin (SST) on insulin secretion and islet proliferation by regulating a specific set of pancreatic genes. Methods To identify SSTR5-regulated pancreatic genes, gene expression microarray analysis was performed on the whole pancreas of 1- and 3-month-old wild-type (WT) and SSTR5 knockout (SSTR5−/−) male mice. Real-time RT-PCR and immunofluorescence were performed to validate selected differentially expressed genes. Results A set of 143 probes were identified to be differentially expressed in the pancreas of 1-month-old SSTR5−/− mice, 72 of which were downregulated and 71 upregulated. At 3 months of age, SSTR5 gene ablation resulted in downregulation of a set of 30 probes and upregulation of a set of 37 probes. Among these differentially expressed genes, there were 15 and 5 genes that were upregulated and downregulated, respectively, in mice at both 1 and 3 months of age. Three genes, PAP/INGAP, ANG, and TDE1, were selected to be validated by real-time RT-PCR and immunofluorescence. Conclusions A specific set of genes linked to a wide range of cellular functions such as islet proliferation, apoptosis, angiogenesis, and tumorigenesis were either upregulated or downregulated in SSTR5-deficient male mice compared with their expression in wild-type mice. Therefore, these genes are potential SSTR5-regulated genes during normal pancreatic development and functional maintenance. ANG1 Expression (dpeaa)DE-He213 Gene Expression Alteration (dpeaa)DE-He213 Islet Neogenesis (dpeaa)DE-He213 Islet Hyperplasia (dpeaa)DE-He213 Islet Cell Proliferation (dpeaa)DE-He213 Zhou, Guisheng aut Liu, Shi-He aut Li, Min aut Jeong, Jae-Wook aut DeMayo, Francesco J. aut Gingras, Marie-Claude aut Gibbs, Richard A. aut Fisher, William E. aut Brunicardi, F. Charles aut Enthalten in World Journal of Surgery Springer-Verlag, 1996 33(2009), 4 vom: 10. Jan. (DE-627)SPR003391159 nnns volume:33 year:2009 number:4 day:10 month:01 https://dx.doi.org/10.1007/s00268-008-9893-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 33 2009 4 10 01 |
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Patel, Sanjeet G. |
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Patel, Sanjeet G. misc ANG1 Expression misc Gene Expression Alteration misc Islet Neogenesis misc Islet Hyperplasia misc Islet Cell Proliferation Microarray Analysis of Somatostatin Receptor 5-Regulated Gene Expression Profiles in Murine Pancreas |
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Microarray Analysis of Somatostatin Receptor 5-Regulated Gene Expression Profiles in Murine Pancreas ANG1 Expression (dpeaa)DE-He213 Gene Expression Alteration (dpeaa)DE-He213 Islet Neogenesis (dpeaa)DE-He213 Islet Hyperplasia (dpeaa)DE-He213 Islet Cell Proliferation (dpeaa)DE-He213 |
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Microarray Analysis of Somatostatin Receptor 5-Regulated Gene Expression Profiles in Murine Pancreas |
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Microarray Analysis of Somatostatin Receptor 5-Regulated Gene Expression Profiles in Murine Pancreas |
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World Journal of Surgery |
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2009 |
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Patel, Sanjeet G. Zhou, Guisheng Liu, Shi-He Li, Min Jeong, Jae-Wook DeMayo, Francesco J. Gingras, Marie-Claude Gibbs, Richard A. Fisher, William E. Brunicardi, F. Charles |
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Patel, Sanjeet G. |
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10.1007/s00268-008-9893-1 |
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microarray analysis of somatostatin receptor 5-regulated gene expression profiles in murine pancreas |
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Microarray Analysis of Somatostatin Receptor 5-Regulated Gene Expression Profiles in Murine Pancreas |
| abstract |
Background We previously demonstrated that somatostatin receptor type 5 (SSTR5) gene ablation results in alterations in insulin secretion and glucose metabolism, accompanied by morphologic alterations in the islets of Langerhans. The underlying mechanism(s) by which SSTR5 exerts its cellular functions remain(s) unknown. We hypothesized that SSTR5 mediates the inhibitory effect of somatostatin (SST) on insulin secretion and islet proliferation by regulating a specific set of pancreatic genes. Methods To identify SSTR5-regulated pancreatic genes, gene expression microarray analysis was performed on the whole pancreas of 1- and 3-month-old wild-type (WT) and SSTR5 knockout (SSTR5−/−) male mice. Real-time RT-PCR and immunofluorescence were performed to validate selected differentially expressed genes. Results A set of 143 probes were identified to be differentially expressed in the pancreas of 1-month-old SSTR5−/− mice, 72 of which were downregulated and 71 upregulated. At 3 months of age, SSTR5 gene ablation resulted in downregulation of a set of 30 probes and upregulation of a set of 37 probes. Among these differentially expressed genes, there were 15 and 5 genes that were upregulated and downregulated, respectively, in mice at both 1 and 3 months of age. Three genes, PAP/INGAP, ANG, and TDE1, were selected to be validated by real-time RT-PCR and immunofluorescence. Conclusions A specific set of genes linked to a wide range of cellular functions such as islet proliferation, apoptosis, angiogenesis, and tumorigenesis were either upregulated or downregulated in SSTR5-deficient male mice compared with their expression in wild-type mice. Therefore, these genes are potential SSTR5-regulated genes during normal pancreatic development and functional maintenance. © Société Internationale de Chirurgie 2009 |
| abstractGer |
Background We previously demonstrated that somatostatin receptor type 5 (SSTR5) gene ablation results in alterations in insulin secretion and glucose metabolism, accompanied by morphologic alterations in the islets of Langerhans. The underlying mechanism(s) by which SSTR5 exerts its cellular functions remain(s) unknown. We hypothesized that SSTR5 mediates the inhibitory effect of somatostatin (SST) on insulin secretion and islet proliferation by regulating a specific set of pancreatic genes. Methods To identify SSTR5-regulated pancreatic genes, gene expression microarray analysis was performed on the whole pancreas of 1- and 3-month-old wild-type (WT) and SSTR5 knockout (SSTR5−/−) male mice. Real-time RT-PCR and immunofluorescence were performed to validate selected differentially expressed genes. Results A set of 143 probes were identified to be differentially expressed in the pancreas of 1-month-old SSTR5−/− mice, 72 of which were downregulated and 71 upregulated. At 3 months of age, SSTR5 gene ablation resulted in downregulation of a set of 30 probes and upregulation of a set of 37 probes. Among these differentially expressed genes, there were 15 and 5 genes that were upregulated and downregulated, respectively, in mice at both 1 and 3 months of age. Three genes, PAP/INGAP, ANG, and TDE1, were selected to be validated by real-time RT-PCR and immunofluorescence. Conclusions A specific set of genes linked to a wide range of cellular functions such as islet proliferation, apoptosis, angiogenesis, and tumorigenesis were either upregulated or downregulated in SSTR5-deficient male mice compared with their expression in wild-type mice. Therefore, these genes are potential SSTR5-regulated genes during normal pancreatic development and functional maintenance. © Société Internationale de Chirurgie 2009 |
| abstract_unstemmed |
Background We previously demonstrated that somatostatin receptor type 5 (SSTR5) gene ablation results in alterations in insulin secretion and glucose metabolism, accompanied by morphologic alterations in the islets of Langerhans. The underlying mechanism(s) by which SSTR5 exerts its cellular functions remain(s) unknown. We hypothesized that SSTR5 mediates the inhibitory effect of somatostatin (SST) on insulin secretion and islet proliferation by regulating a specific set of pancreatic genes. Methods To identify SSTR5-regulated pancreatic genes, gene expression microarray analysis was performed on the whole pancreas of 1- and 3-month-old wild-type (WT) and SSTR5 knockout (SSTR5−/−) male mice. Real-time RT-PCR and immunofluorescence were performed to validate selected differentially expressed genes. Results A set of 143 probes were identified to be differentially expressed in the pancreas of 1-month-old SSTR5−/− mice, 72 of which were downregulated and 71 upregulated. At 3 months of age, SSTR5 gene ablation resulted in downregulation of a set of 30 probes and upregulation of a set of 37 probes. Among these differentially expressed genes, there were 15 and 5 genes that were upregulated and downregulated, respectively, in mice at both 1 and 3 months of age. Three genes, PAP/INGAP, ANG, and TDE1, were selected to be validated by real-time RT-PCR and immunofluorescence. Conclusions A specific set of genes linked to a wide range of cellular functions such as islet proliferation, apoptosis, angiogenesis, and tumorigenesis were either upregulated or downregulated in SSTR5-deficient male mice compared with their expression in wild-type mice. Therefore, these genes are potential SSTR5-regulated genes during normal pancreatic development and functional maintenance. © Société Internationale de Chirurgie 2009 |
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Microarray Analysis of Somatostatin Receptor 5-Regulated Gene Expression Profiles in Murine Pancreas |
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https://dx.doi.org/10.1007/s00268-008-9893-1 |
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Zhou, Guisheng Liu, Shi-He Li, Min Jeong, Jae-Wook DeMayo, Francesco J. Gingras, Marie-Claude Gibbs, Richard A. Fisher, William E. Brunicardi, F. Charles |
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Zhou, Guisheng Liu, Shi-He Li, Min Jeong, Jae-Wook DeMayo, Francesco J. Gingras, Marie-Claude Gibbs, Richard A. Fisher, William E. Brunicardi, F. Charles |
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