HMBA induces cell death and potentiates doxorubicin toxicity in malignant mesothelioma cells
Purpose Malignant pleural mesothelioma (MM), a rare tumor characterized by high local invasiveness and low metastatic efficiency, is poorly responsive to current therapeutic approaches. The aim of the present study was to evaluate the cytotoxic efficacy of the hybrid polar compound hexamethylene bis...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Palumbo, Camilla [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2004 |
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| Schlagwörter: |
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| Anmerkung: |
© Springer-Verlag 2004 |
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| Übergeordnetes Werk: |
Enthalten in: Cancer chemotherapy and pharmacology - Berlin : Springer, 1978, 54(2004), 5 vom: 26. Juni, Seite 398-406 |
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| Übergeordnetes Werk: |
volume:54 ; year:2004 ; number:5 ; day:26 ; month:06 ; pages:398-406 |
| Links: |
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| DOI / URN: |
10.1007/s00280-004-0838-6 |
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| Katalog-ID: |
SPR003581896 |
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| 100 | 1 | |a Palumbo, Camilla |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a HMBA induces cell death and potentiates doxorubicin toxicity in malignant mesothelioma cells |
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| 520 | |a Purpose Malignant pleural mesothelioma (MM), a rare tumor characterized by high local invasiveness and low metastatic efficiency, is poorly responsive to current therapeutic approaches. The aim of the present study was to evaluate the cytotoxic efficacy of the hybrid polar compound hexamethylene bisacetamide (HMBA), either as a single agent or in combination with the anthracycline doxorubicin (DOX), against MM cells. Methods The MM cell lines MM-B1 and MM-E1 were treated with HMBA, DOX or with combinations of the two drugs. Cell survival and death were assessed by the MTS assay and trypan blue staining/TUNEL, respectively. The interactions between drugs were evaluated by the method of Kern et al. Western blot analysis was used to investigate the expression of Bcl-2 family proteins. Results When administered alone, HMBA dose-dependently decreased the number of viable cells and increased the death rate of MM-B1 and MM-E1 cultures. Combinations of HMBA and DOX achieved a synergistic inhibition of MM cell survival, and the simultaneous administration of HMBA counteracted the resistance induced by DOX in MM-E1 cells. HMBA, used at cytostatic concentrations, reduced the ratio between antiapoptotic (Bcl-2, Bcl-$ X_{L} $) and proapoptotic (Bax) members of the Bcl-2 family of proteins, thus lowering the threshold for MM cell death commitment. Conclusions HMBA has therapeutic potential in MM both as a single agent and through potentiation of DOX toxicity. These results support future investigations on the feasibility of intrapleural chemotherapy with this hybrid polar compound. | ||
| 650 | 4 | |a Mesothelioma |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a HMBA |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Doxorubicin |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Drug synergism |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Intrapleural chemotherapy |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Albonici, Loredana |4 aut | |
| 700 | 1 | |a Bei, Roberto |4 aut | |
| 700 | 1 | |a Bocci, Chiara |4 aut | |
| 700 | 1 | |a Scarpa, Susanna |4 aut | |
| 700 | 1 | |a Di Nardo, Paolo |4 aut | |
| 700 | 1 | |a Modesti, Andrea |4 aut | |
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10.1007/s00280-004-0838-6 doi (DE-627)SPR003581896 (SPR)s00280-004-0838-6-e DE-627 ger DE-627 rakwb eng Palumbo, Camilla verfasserin aut HMBA induces cell death and potentiates doxorubicin toxicity in malignant mesothelioma cells 2004 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag 2004 Purpose Malignant pleural mesothelioma (MM), a rare tumor characterized by high local invasiveness and low metastatic efficiency, is poorly responsive to current therapeutic approaches. The aim of the present study was to evaluate the cytotoxic efficacy of the hybrid polar compound hexamethylene bisacetamide (HMBA), either as a single agent or in combination with the anthracycline doxorubicin (DOX), against MM cells. Methods The MM cell lines MM-B1 and MM-E1 were treated with HMBA, DOX or with combinations of the two drugs. Cell survival and death were assessed by the MTS assay and trypan blue staining/TUNEL, respectively. The interactions between drugs were evaluated by the method of Kern et al. Western blot analysis was used to investigate the expression of Bcl-2 family proteins. Results When administered alone, HMBA dose-dependently decreased the number of viable cells and increased the death rate of MM-B1 and MM-E1 cultures. Combinations of HMBA and DOX achieved a synergistic inhibition of MM cell survival, and the simultaneous administration of HMBA counteracted the resistance induced by DOX in MM-E1 cells. HMBA, used at cytostatic concentrations, reduced the ratio between antiapoptotic (Bcl-2, Bcl-$ X_{L} $) and proapoptotic (Bax) members of the Bcl-2 family of proteins, thus lowering the threshold for MM cell death commitment. Conclusions HMBA has therapeutic potential in MM both as a single agent and through potentiation of DOX toxicity. These results support future investigations on the feasibility of intrapleural chemotherapy with this hybrid polar compound. Mesothelioma (dpeaa)DE-He213 HMBA (dpeaa)DE-He213 Doxorubicin (dpeaa)DE-He213 Drug synergism (dpeaa)DE-He213 Intrapleural chemotherapy (dpeaa)DE-He213 Albonici, Loredana aut Bei, Roberto aut Bocci, Chiara aut Scarpa, Susanna aut Di Nardo, Paolo aut Modesti, Andrea aut Enthalten in Cancer chemotherapy and pharmacology Berlin : Springer, 1978 54(2004), 5 vom: 26. Juni, Seite 398-406 (DE-627)253390435 (DE-600)1458488-8 1432-0843 nnns volume:54 year:2004 number:5 day:26 month:06 pages:398-406 https://dx.doi.org/10.1007/s00280-004-0838-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_711 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 54 2004 5 26 06 398-406 |
| spelling |
10.1007/s00280-004-0838-6 doi (DE-627)SPR003581896 (SPR)s00280-004-0838-6-e DE-627 ger DE-627 rakwb eng Palumbo, Camilla verfasserin aut HMBA induces cell death and potentiates doxorubicin toxicity in malignant mesothelioma cells 2004 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag 2004 Purpose Malignant pleural mesothelioma (MM), a rare tumor characterized by high local invasiveness and low metastatic efficiency, is poorly responsive to current therapeutic approaches. The aim of the present study was to evaluate the cytotoxic efficacy of the hybrid polar compound hexamethylene bisacetamide (HMBA), either as a single agent or in combination with the anthracycline doxorubicin (DOX), against MM cells. Methods The MM cell lines MM-B1 and MM-E1 were treated with HMBA, DOX or with combinations of the two drugs. Cell survival and death were assessed by the MTS assay and trypan blue staining/TUNEL, respectively. The interactions between drugs were evaluated by the method of Kern et al. Western blot analysis was used to investigate the expression of Bcl-2 family proteins. Results When administered alone, HMBA dose-dependently decreased the number of viable cells and increased the death rate of MM-B1 and MM-E1 cultures. Combinations of HMBA and DOX achieved a synergistic inhibition of MM cell survival, and the simultaneous administration of HMBA counteracted the resistance induced by DOX in MM-E1 cells. HMBA, used at cytostatic concentrations, reduced the ratio between antiapoptotic (Bcl-2, Bcl-$ X_{L} $) and proapoptotic (Bax) members of the Bcl-2 family of proteins, thus lowering the threshold for MM cell death commitment. Conclusions HMBA has therapeutic potential in MM both as a single agent and through potentiation of DOX toxicity. These results support future investigations on the feasibility of intrapleural chemotherapy with this hybrid polar compound. Mesothelioma (dpeaa)DE-He213 HMBA (dpeaa)DE-He213 Doxorubicin (dpeaa)DE-He213 Drug synergism (dpeaa)DE-He213 Intrapleural chemotherapy (dpeaa)DE-He213 Albonici, Loredana aut Bei, Roberto aut Bocci, Chiara aut Scarpa, Susanna aut Di Nardo, Paolo aut Modesti, Andrea aut Enthalten in Cancer chemotherapy and pharmacology Berlin : Springer, 1978 54(2004), 5 vom: 26. Juni, Seite 398-406 (DE-627)253390435 (DE-600)1458488-8 1432-0843 nnns volume:54 year:2004 number:5 day:26 month:06 pages:398-406 https://dx.doi.org/10.1007/s00280-004-0838-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_711 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 54 2004 5 26 06 398-406 |
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10.1007/s00280-004-0838-6 doi (DE-627)SPR003581896 (SPR)s00280-004-0838-6-e DE-627 ger DE-627 rakwb eng Palumbo, Camilla verfasserin aut HMBA induces cell death and potentiates doxorubicin toxicity in malignant mesothelioma cells 2004 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag 2004 Purpose Malignant pleural mesothelioma (MM), a rare tumor characterized by high local invasiveness and low metastatic efficiency, is poorly responsive to current therapeutic approaches. The aim of the present study was to evaluate the cytotoxic efficacy of the hybrid polar compound hexamethylene bisacetamide (HMBA), either as a single agent or in combination with the anthracycline doxorubicin (DOX), against MM cells. Methods The MM cell lines MM-B1 and MM-E1 were treated with HMBA, DOX or with combinations of the two drugs. Cell survival and death were assessed by the MTS assay and trypan blue staining/TUNEL, respectively. The interactions between drugs were evaluated by the method of Kern et al. Western blot analysis was used to investigate the expression of Bcl-2 family proteins. Results When administered alone, HMBA dose-dependently decreased the number of viable cells and increased the death rate of MM-B1 and MM-E1 cultures. Combinations of HMBA and DOX achieved a synergistic inhibition of MM cell survival, and the simultaneous administration of HMBA counteracted the resistance induced by DOX in MM-E1 cells. HMBA, used at cytostatic concentrations, reduced the ratio between antiapoptotic (Bcl-2, Bcl-$ X_{L} $) and proapoptotic (Bax) members of the Bcl-2 family of proteins, thus lowering the threshold for MM cell death commitment. Conclusions HMBA has therapeutic potential in MM both as a single agent and through potentiation of DOX toxicity. These results support future investigations on the feasibility of intrapleural chemotherapy with this hybrid polar compound. Mesothelioma (dpeaa)DE-He213 HMBA (dpeaa)DE-He213 Doxorubicin (dpeaa)DE-He213 Drug synergism (dpeaa)DE-He213 Intrapleural chemotherapy (dpeaa)DE-He213 Albonici, Loredana aut Bei, Roberto aut Bocci, Chiara aut Scarpa, Susanna aut Di Nardo, Paolo aut Modesti, Andrea aut Enthalten in Cancer chemotherapy and pharmacology Berlin : Springer, 1978 54(2004), 5 vom: 26. Juni, Seite 398-406 (DE-627)253390435 (DE-600)1458488-8 1432-0843 nnns volume:54 year:2004 number:5 day:26 month:06 pages:398-406 https://dx.doi.org/10.1007/s00280-004-0838-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_711 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 54 2004 5 26 06 398-406 |
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10.1007/s00280-004-0838-6 doi (DE-627)SPR003581896 (SPR)s00280-004-0838-6-e DE-627 ger DE-627 rakwb eng Palumbo, Camilla verfasserin aut HMBA induces cell death and potentiates doxorubicin toxicity in malignant mesothelioma cells 2004 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag 2004 Purpose Malignant pleural mesothelioma (MM), a rare tumor characterized by high local invasiveness and low metastatic efficiency, is poorly responsive to current therapeutic approaches. The aim of the present study was to evaluate the cytotoxic efficacy of the hybrid polar compound hexamethylene bisacetamide (HMBA), either as a single agent or in combination with the anthracycline doxorubicin (DOX), against MM cells. Methods The MM cell lines MM-B1 and MM-E1 were treated with HMBA, DOX or with combinations of the two drugs. Cell survival and death were assessed by the MTS assay and trypan blue staining/TUNEL, respectively. The interactions between drugs were evaluated by the method of Kern et al. Western blot analysis was used to investigate the expression of Bcl-2 family proteins. Results When administered alone, HMBA dose-dependently decreased the number of viable cells and increased the death rate of MM-B1 and MM-E1 cultures. Combinations of HMBA and DOX achieved a synergistic inhibition of MM cell survival, and the simultaneous administration of HMBA counteracted the resistance induced by DOX in MM-E1 cells. HMBA, used at cytostatic concentrations, reduced the ratio between antiapoptotic (Bcl-2, Bcl-$ X_{L} $) and proapoptotic (Bax) members of the Bcl-2 family of proteins, thus lowering the threshold for MM cell death commitment. Conclusions HMBA has therapeutic potential in MM both as a single agent and through potentiation of DOX toxicity. These results support future investigations on the feasibility of intrapleural chemotherapy with this hybrid polar compound. Mesothelioma (dpeaa)DE-He213 HMBA (dpeaa)DE-He213 Doxorubicin (dpeaa)DE-He213 Drug synergism (dpeaa)DE-He213 Intrapleural chemotherapy (dpeaa)DE-He213 Albonici, Loredana aut Bei, Roberto aut Bocci, Chiara aut Scarpa, Susanna aut Di Nardo, Paolo aut Modesti, Andrea aut Enthalten in Cancer chemotherapy and pharmacology Berlin : Springer, 1978 54(2004), 5 vom: 26. Juni, Seite 398-406 (DE-627)253390435 (DE-600)1458488-8 1432-0843 nnns volume:54 year:2004 number:5 day:26 month:06 pages:398-406 https://dx.doi.org/10.1007/s00280-004-0838-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_711 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 54 2004 5 26 06 398-406 |
| allfieldsSound |
10.1007/s00280-004-0838-6 doi (DE-627)SPR003581896 (SPR)s00280-004-0838-6-e DE-627 ger DE-627 rakwb eng Palumbo, Camilla verfasserin aut HMBA induces cell death and potentiates doxorubicin toxicity in malignant mesothelioma cells 2004 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag 2004 Purpose Malignant pleural mesothelioma (MM), a rare tumor characterized by high local invasiveness and low metastatic efficiency, is poorly responsive to current therapeutic approaches. The aim of the present study was to evaluate the cytotoxic efficacy of the hybrid polar compound hexamethylene bisacetamide (HMBA), either as a single agent or in combination with the anthracycline doxorubicin (DOX), against MM cells. Methods The MM cell lines MM-B1 and MM-E1 were treated with HMBA, DOX or with combinations of the two drugs. Cell survival and death were assessed by the MTS assay and trypan blue staining/TUNEL, respectively. The interactions between drugs were evaluated by the method of Kern et al. Western blot analysis was used to investigate the expression of Bcl-2 family proteins. Results When administered alone, HMBA dose-dependently decreased the number of viable cells and increased the death rate of MM-B1 and MM-E1 cultures. Combinations of HMBA and DOX achieved a synergistic inhibition of MM cell survival, and the simultaneous administration of HMBA counteracted the resistance induced by DOX in MM-E1 cells. HMBA, used at cytostatic concentrations, reduced the ratio between antiapoptotic (Bcl-2, Bcl-$ X_{L} $) and proapoptotic (Bax) members of the Bcl-2 family of proteins, thus lowering the threshold for MM cell death commitment. Conclusions HMBA has therapeutic potential in MM both as a single agent and through potentiation of DOX toxicity. These results support future investigations on the feasibility of intrapleural chemotherapy with this hybrid polar compound. Mesothelioma (dpeaa)DE-He213 HMBA (dpeaa)DE-He213 Doxorubicin (dpeaa)DE-He213 Drug synergism (dpeaa)DE-He213 Intrapleural chemotherapy (dpeaa)DE-He213 Albonici, Loredana aut Bei, Roberto aut Bocci, Chiara aut Scarpa, Susanna aut Di Nardo, Paolo aut Modesti, Andrea aut Enthalten in Cancer chemotherapy and pharmacology Berlin : Springer, 1978 54(2004), 5 vom: 26. Juni, Seite 398-406 (DE-627)253390435 (DE-600)1458488-8 1432-0843 nnns volume:54 year:2004 number:5 day:26 month:06 pages:398-406 https://dx.doi.org/10.1007/s00280-004-0838-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_711 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 54 2004 5 26 06 398-406 |
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English |
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Enthalten in Cancer chemotherapy and pharmacology 54(2004), 5 vom: 26. Juni, Seite 398-406 volume:54 year:2004 number:5 day:26 month:06 pages:398-406 |
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Enthalten in Cancer chemotherapy and pharmacology 54(2004), 5 vom: 26. Juni, Seite 398-406 volume:54 year:2004 number:5 day:26 month:06 pages:398-406 |
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Mesothelioma HMBA Doxorubicin Drug synergism Intrapleural chemotherapy |
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Cancer chemotherapy and pharmacology |
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Palumbo, Camilla @@aut@@ Albonici, Loredana @@aut@@ Bei, Roberto @@aut@@ Bocci, Chiara @@aut@@ Scarpa, Susanna @@aut@@ Di Nardo, Paolo @@aut@@ Modesti, Andrea @@aut@@ |
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2004-06-26T00:00:00Z |
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The aim of the present study was to evaluate the cytotoxic efficacy of the hybrid polar compound hexamethylene bisacetamide (HMBA), either as a single agent or in combination with the anthracycline doxorubicin (DOX), against MM cells. Methods The MM cell lines MM-B1 and MM-E1 were treated with HMBA, DOX or with combinations of the two drugs. Cell survival and death were assessed by the MTS assay and trypan blue staining/TUNEL, respectively. The interactions between drugs were evaluated by the method of Kern et al. Western blot analysis was used to investigate the expression of Bcl-2 family proteins. Results When administered alone, HMBA dose-dependently decreased the number of viable cells and increased the death rate of MM-B1 and MM-E1 cultures. Combinations of HMBA and DOX achieved a synergistic inhibition of MM cell survival, and the simultaneous administration of HMBA counteracted the resistance induced by DOX in MM-E1 cells. HMBA, used at cytostatic concentrations, reduced the ratio between antiapoptotic (Bcl-2, Bcl-$ X_{L} $) and proapoptotic (Bax) members of the Bcl-2 family of proteins, thus lowering the threshold for MM cell death commitment. Conclusions HMBA has therapeutic potential in MM both as a single agent and through potentiation of DOX toxicity. 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Palumbo, Camilla |
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Palumbo, Camilla misc Mesothelioma misc HMBA misc Doxorubicin misc Drug synergism misc Intrapleural chemotherapy HMBA induces cell death and potentiates doxorubicin toxicity in malignant mesothelioma cells |
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HMBA induces cell death and potentiates doxorubicin toxicity in malignant mesothelioma cells Mesothelioma (dpeaa)DE-He213 HMBA (dpeaa)DE-He213 Doxorubicin (dpeaa)DE-He213 Drug synergism (dpeaa)DE-He213 Intrapleural chemotherapy (dpeaa)DE-He213 |
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HMBA induces cell death and potentiates doxorubicin toxicity in malignant mesothelioma cells |
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HMBA induces cell death and potentiates doxorubicin toxicity in malignant mesothelioma cells |
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Palumbo, Camilla Albonici, Loredana Bei, Roberto Bocci, Chiara Scarpa, Susanna Di Nardo, Paolo Modesti, Andrea |
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hmba induces cell death and potentiates doxorubicin toxicity in malignant mesothelioma cells |
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HMBA induces cell death and potentiates doxorubicin toxicity in malignant mesothelioma cells |
| abstract |
Purpose Malignant pleural mesothelioma (MM), a rare tumor characterized by high local invasiveness and low metastatic efficiency, is poorly responsive to current therapeutic approaches. The aim of the present study was to evaluate the cytotoxic efficacy of the hybrid polar compound hexamethylene bisacetamide (HMBA), either as a single agent or in combination with the anthracycline doxorubicin (DOX), against MM cells. Methods The MM cell lines MM-B1 and MM-E1 were treated with HMBA, DOX or with combinations of the two drugs. Cell survival and death were assessed by the MTS assay and trypan blue staining/TUNEL, respectively. The interactions between drugs were evaluated by the method of Kern et al. Western blot analysis was used to investigate the expression of Bcl-2 family proteins. Results When administered alone, HMBA dose-dependently decreased the number of viable cells and increased the death rate of MM-B1 and MM-E1 cultures. Combinations of HMBA and DOX achieved a synergistic inhibition of MM cell survival, and the simultaneous administration of HMBA counteracted the resistance induced by DOX in MM-E1 cells. HMBA, used at cytostatic concentrations, reduced the ratio between antiapoptotic (Bcl-2, Bcl-$ X_{L} $) and proapoptotic (Bax) members of the Bcl-2 family of proteins, thus lowering the threshold for MM cell death commitment. Conclusions HMBA has therapeutic potential in MM both as a single agent and through potentiation of DOX toxicity. These results support future investigations on the feasibility of intrapleural chemotherapy with this hybrid polar compound. © Springer-Verlag 2004 |
| abstractGer |
Purpose Malignant pleural mesothelioma (MM), a rare tumor characterized by high local invasiveness and low metastatic efficiency, is poorly responsive to current therapeutic approaches. The aim of the present study was to evaluate the cytotoxic efficacy of the hybrid polar compound hexamethylene bisacetamide (HMBA), either as a single agent or in combination with the anthracycline doxorubicin (DOX), against MM cells. Methods The MM cell lines MM-B1 and MM-E1 were treated with HMBA, DOX or with combinations of the two drugs. Cell survival and death were assessed by the MTS assay and trypan blue staining/TUNEL, respectively. The interactions between drugs were evaluated by the method of Kern et al. Western blot analysis was used to investigate the expression of Bcl-2 family proteins. Results When administered alone, HMBA dose-dependently decreased the number of viable cells and increased the death rate of MM-B1 and MM-E1 cultures. Combinations of HMBA and DOX achieved a synergistic inhibition of MM cell survival, and the simultaneous administration of HMBA counteracted the resistance induced by DOX in MM-E1 cells. HMBA, used at cytostatic concentrations, reduced the ratio between antiapoptotic (Bcl-2, Bcl-$ X_{L} $) and proapoptotic (Bax) members of the Bcl-2 family of proteins, thus lowering the threshold for MM cell death commitment. Conclusions HMBA has therapeutic potential in MM both as a single agent and through potentiation of DOX toxicity. These results support future investigations on the feasibility of intrapleural chemotherapy with this hybrid polar compound. © Springer-Verlag 2004 |
| abstract_unstemmed |
Purpose Malignant pleural mesothelioma (MM), a rare tumor characterized by high local invasiveness and low metastatic efficiency, is poorly responsive to current therapeutic approaches. The aim of the present study was to evaluate the cytotoxic efficacy of the hybrid polar compound hexamethylene bisacetamide (HMBA), either as a single agent or in combination with the anthracycline doxorubicin (DOX), against MM cells. Methods The MM cell lines MM-B1 and MM-E1 were treated with HMBA, DOX or with combinations of the two drugs. Cell survival and death were assessed by the MTS assay and trypan blue staining/TUNEL, respectively. The interactions between drugs were evaluated by the method of Kern et al. Western blot analysis was used to investigate the expression of Bcl-2 family proteins. Results When administered alone, HMBA dose-dependently decreased the number of viable cells and increased the death rate of MM-B1 and MM-E1 cultures. Combinations of HMBA and DOX achieved a synergistic inhibition of MM cell survival, and the simultaneous administration of HMBA counteracted the resistance induced by DOX in MM-E1 cells. HMBA, used at cytostatic concentrations, reduced the ratio between antiapoptotic (Bcl-2, Bcl-$ X_{L} $) and proapoptotic (Bax) members of the Bcl-2 family of proteins, thus lowering the threshold for MM cell death commitment. Conclusions HMBA has therapeutic potential in MM both as a single agent and through potentiation of DOX toxicity. These results support future investigations on the feasibility of intrapleural chemotherapy with this hybrid polar compound. © Springer-Verlag 2004 |
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| title_short |
HMBA induces cell death and potentiates doxorubicin toxicity in malignant mesothelioma cells |
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https://dx.doi.org/10.1007/s00280-004-0838-6 |
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Albonici, Loredana Bei, Roberto Bocci, Chiara Scarpa, Susanna Di Nardo, Paolo Modesti, Andrea |
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| score |
7.397806 |