Carbon-13 (13C) Labeling of Bacillus subtilis Vegetative Cells and Spores: Suitability for DNA Stable Isotope Probing (DNA-SIP) of Spores in Soils
Abstract To test the suitability of DNA stable isotope probing (DNA-SIP) for characterizing bacterial spore populations in soils, the properties of Bacillus subtilis cells and spores intensely labeled with [13C]glucose were characterized. Spore germination, vegetative growth rates, and sporulation e...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Nicholson, Wayne L. [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2009 |
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| Schlagwörter: |
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| Anmerkung: |
© Springer Science+Business Media, LLC 2009 |
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| Übergeordnetes Werk: |
Enthalten in: Current microbiology - New York, NY : Springer, 1978, 59(2009), 1 vom: 11. März, Seite 9-14 |
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| Übergeordnetes Werk: |
volume:59 ; year:2009 ; number:1 ; day:11 ; month:03 ; pages:9-14 |
| Links: |
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| DOI / URN: |
10.1007/s00284-009-9387-x |
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| Katalog-ID: |
SPR003669017 |
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| 100 | 1 | |a Nicholson, Wayne L. |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Carbon-13 (13C) Labeling of Bacillus subtilis Vegetative Cells and Spores: Suitability for DNA Stable Isotope Probing (DNA-SIP) of Spores in Soils |
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| 520 | |a Abstract To test the suitability of DNA stable isotope probing (DNA-SIP) for characterizing bacterial spore populations in soils, the properties of Bacillus subtilis cells and spores intensely labeled with [13C]glucose were characterized. Spore germination, vegetative growth rates, and sporulation efficiency were indistinguishable on glucose versus [13C]glucose, as were spore wet heat and UV resistance. Unlabeled and 13C-labeled spores contained 1.0989 and 74.336 at.% 13C, and exhibited wet densities of 1.356 and 1.365 g/ml, respectively. Chromosomal DNAs containing 12C versus 13C were readily separated by their different buoyant densities in cesium chloride/ethidium bromide gradients. | ||
| 650 | 4 | |a EtBr |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Bacterial Spore |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Stable Isotope Probe |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Dormant Spore |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Spore Yield |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Fedenko, Jeffrey |4 aut | |
| 700 | 1 | |a Schuerger, Andrew C. |4 aut | |
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| 912 | |a GBV_ILN_2048 | ||
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| 912 | |a GBV_ILN_2055 | ||
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| 912 | |a GBV_ILN_2059 | ||
| 912 | |a GBV_ILN_2061 | ||
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| 912 | |a GBV_ILN_2065 | ||
| 912 | |a GBV_ILN_2068 | ||
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| 912 | |a GBV_ILN_2086 | ||
| 912 | |a GBV_ILN_2088 | ||
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| 912 | |a GBV_ILN_2106 | ||
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| 912 | |a GBV_ILN_2110 | ||
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10.1007/s00284-009-9387-x doi (DE-627)SPR003669017 (SPR)s00284-009-9387-x-e DE-627 ger DE-627 rakwb eng Nicholson, Wayne L. verfasserin aut Carbon-13 (13C) Labeling of Bacillus subtilis Vegetative Cells and Spores: Suitability for DNA Stable Isotope Probing (DNA-SIP) of Spores in Soils 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer Science+Business Media, LLC 2009 Abstract To test the suitability of DNA stable isotope probing (DNA-SIP) for characterizing bacterial spore populations in soils, the properties of Bacillus subtilis cells and spores intensely labeled with [13C]glucose were characterized. Spore germination, vegetative growth rates, and sporulation efficiency were indistinguishable on glucose versus [13C]glucose, as were spore wet heat and UV resistance. Unlabeled and 13C-labeled spores contained 1.0989 and 74.336 at.% 13C, and exhibited wet densities of 1.356 and 1.365 g/ml, respectively. Chromosomal DNAs containing 12C versus 13C were readily separated by their different buoyant densities in cesium chloride/ethidium bromide gradients. EtBr (dpeaa)DE-He213 Bacterial Spore (dpeaa)DE-He213 Stable Isotope Probe (dpeaa)DE-He213 Dormant Spore (dpeaa)DE-He213 Spore Yield (dpeaa)DE-He213 Fedenko, Jeffrey aut Schuerger, Andrew C. aut Enthalten in Current microbiology New York, NY : Springer, 1978 59(2009), 1 vom: 11. März, Seite 9-14 (DE-627)253722160 (DE-600)1458987-4 1432-0991 nnns volume:59 year:2009 number:1 day:11 month:03 pages:9-14 https://dx.doi.org/10.1007/s00284-009-9387-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 59 2009 1 11 03 9-14 |
| spelling |
10.1007/s00284-009-9387-x doi (DE-627)SPR003669017 (SPR)s00284-009-9387-x-e DE-627 ger DE-627 rakwb eng Nicholson, Wayne L. verfasserin aut Carbon-13 (13C) Labeling of Bacillus subtilis Vegetative Cells and Spores: Suitability for DNA Stable Isotope Probing (DNA-SIP) of Spores in Soils 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer Science+Business Media, LLC 2009 Abstract To test the suitability of DNA stable isotope probing (DNA-SIP) for characterizing bacterial spore populations in soils, the properties of Bacillus subtilis cells and spores intensely labeled with [13C]glucose were characterized. Spore germination, vegetative growth rates, and sporulation efficiency were indistinguishable on glucose versus [13C]glucose, as were spore wet heat and UV resistance. Unlabeled and 13C-labeled spores contained 1.0989 and 74.336 at.% 13C, and exhibited wet densities of 1.356 and 1.365 g/ml, respectively. Chromosomal DNAs containing 12C versus 13C were readily separated by their different buoyant densities in cesium chloride/ethidium bromide gradients. EtBr (dpeaa)DE-He213 Bacterial Spore (dpeaa)DE-He213 Stable Isotope Probe (dpeaa)DE-He213 Dormant Spore (dpeaa)DE-He213 Spore Yield (dpeaa)DE-He213 Fedenko, Jeffrey aut Schuerger, Andrew C. aut Enthalten in Current microbiology New York, NY : Springer, 1978 59(2009), 1 vom: 11. März, Seite 9-14 (DE-627)253722160 (DE-600)1458987-4 1432-0991 nnns volume:59 year:2009 number:1 day:11 month:03 pages:9-14 https://dx.doi.org/10.1007/s00284-009-9387-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 59 2009 1 11 03 9-14 |
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10.1007/s00284-009-9387-x doi (DE-627)SPR003669017 (SPR)s00284-009-9387-x-e DE-627 ger DE-627 rakwb eng Nicholson, Wayne L. verfasserin aut Carbon-13 (13C) Labeling of Bacillus subtilis Vegetative Cells and Spores: Suitability for DNA Stable Isotope Probing (DNA-SIP) of Spores in Soils 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer Science+Business Media, LLC 2009 Abstract To test the suitability of DNA stable isotope probing (DNA-SIP) for characterizing bacterial spore populations in soils, the properties of Bacillus subtilis cells and spores intensely labeled with [13C]glucose were characterized. Spore germination, vegetative growth rates, and sporulation efficiency were indistinguishable on glucose versus [13C]glucose, as were spore wet heat and UV resistance. Unlabeled and 13C-labeled spores contained 1.0989 and 74.336 at.% 13C, and exhibited wet densities of 1.356 and 1.365 g/ml, respectively. Chromosomal DNAs containing 12C versus 13C were readily separated by their different buoyant densities in cesium chloride/ethidium bromide gradients. EtBr (dpeaa)DE-He213 Bacterial Spore (dpeaa)DE-He213 Stable Isotope Probe (dpeaa)DE-He213 Dormant Spore (dpeaa)DE-He213 Spore Yield (dpeaa)DE-He213 Fedenko, Jeffrey aut Schuerger, Andrew C. aut Enthalten in Current microbiology New York, NY : Springer, 1978 59(2009), 1 vom: 11. März, Seite 9-14 (DE-627)253722160 (DE-600)1458987-4 1432-0991 nnns volume:59 year:2009 number:1 day:11 month:03 pages:9-14 https://dx.doi.org/10.1007/s00284-009-9387-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 59 2009 1 11 03 9-14 |
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10.1007/s00284-009-9387-x doi (DE-627)SPR003669017 (SPR)s00284-009-9387-x-e DE-627 ger DE-627 rakwb eng Nicholson, Wayne L. verfasserin aut Carbon-13 (13C) Labeling of Bacillus subtilis Vegetative Cells and Spores: Suitability for DNA Stable Isotope Probing (DNA-SIP) of Spores in Soils 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer Science+Business Media, LLC 2009 Abstract To test the suitability of DNA stable isotope probing (DNA-SIP) for characterizing bacterial spore populations in soils, the properties of Bacillus subtilis cells and spores intensely labeled with [13C]glucose were characterized. Spore germination, vegetative growth rates, and sporulation efficiency were indistinguishable on glucose versus [13C]glucose, as were spore wet heat and UV resistance. Unlabeled and 13C-labeled spores contained 1.0989 and 74.336 at.% 13C, and exhibited wet densities of 1.356 and 1.365 g/ml, respectively. Chromosomal DNAs containing 12C versus 13C were readily separated by their different buoyant densities in cesium chloride/ethidium bromide gradients. EtBr (dpeaa)DE-He213 Bacterial Spore (dpeaa)DE-He213 Stable Isotope Probe (dpeaa)DE-He213 Dormant Spore (dpeaa)DE-He213 Spore Yield (dpeaa)DE-He213 Fedenko, Jeffrey aut Schuerger, Andrew C. aut Enthalten in Current microbiology New York, NY : Springer, 1978 59(2009), 1 vom: 11. März, Seite 9-14 (DE-627)253722160 (DE-600)1458987-4 1432-0991 nnns volume:59 year:2009 number:1 day:11 month:03 pages:9-14 https://dx.doi.org/10.1007/s00284-009-9387-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 59 2009 1 11 03 9-14 |
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10.1007/s00284-009-9387-x doi (DE-627)SPR003669017 (SPR)s00284-009-9387-x-e DE-627 ger DE-627 rakwb eng Nicholson, Wayne L. verfasserin aut Carbon-13 (13C) Labeling of Bacillus subtilis Vegetative Cells and Spores: Suitability for DNA Stable Isotope Probing (DNA-SIP) of Spores in Soils 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer Science+Business Media, LLC 2009 Abstract To test the suitability of DNA stable isotope probing (DNA-SIP) for characterizing bacterial spore populations in soils, the properties of Bacillus subtilis cells and spores intensely labeled with [13C]glucose were characterized. Spore germination, vegetative growth rates, and sporulation efficiency were indistinguishable on glucose versus [13C]glucose, as were spore wet heat and UV resistance. Unlabeled and 13C-labeled spores contained 1.0989 and 74.336 at.% 13C, and exhibited wet densities of 1.356 and 1.365 g/ml, respectively. Chromosomal DNAs containing 12C versus 13C were readily separated by their different buoyant densities in cesium chloride/ethidium bromide gradients. EtBr (dpeaa)DE-He213 Bacterial Spore (dpeaa)DE-He213 Stable Isotope Probe (dpeaa)DE-He213 Dormant Spore (dpeaa)DE-He213 Spore Yield (dpeaa)DE-He213 Fedenko, Jeffrey aut Schuerger, Andrew C. aut Enthalten in Current microbiology New York, NY : Springer, 1978 59(2009), 1 vom: 11. März, Seite 9-14 (DE-627)253722160 (DE-600)1458987-4 1432-0991 nnns volume:59 year:2009 number:1 day:11 month:03 pages:9-14 https://dx.doi.org/10.1007/s00284-009-9387-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 59 2009 1 11 03 9-14 |
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Nicholson, Wayne L. |
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Nicholson, Wayne L. misc EtBr misc Bacterial Spore misc Stable Isotope Probe misc Dormant Spore misc Spore Yield Carbon-13 (13C) Labeling of Bacillus subtilis Vegetative Cells and Spores: Suitability for DNA Stable Isotope Probing (DNA-SIP) of Spores in Soils |
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Carbon-13 (13C) Labeling of Bacillus subtilis Vegetative Cells and Spores: Suitability for DNA Stable Isotope Probing (DNA-SIP) of Spores in Soils EtBr (dpeaa)DE-He213 Bacterial Spore (dpeaa)DE-He213 Stable Isotope Probe (dpeaa)DE-He213 Dormant Spore (dpeaa)DE-He213 Spore Yield (dpeaa)DE-He213 |
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Carbon-13 (13C) Labeling of Bacillus subtilis Vegetative Cells and Spores: Suitability for DNA Stable Isotope Probing (DNA-SIP) of Spores in Soils |
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Carbon-13 (13C) Labeling of Bacillus subtilis Vegetative Cells and Spores: Suitability for DNA Stable Isotope Probing (DNA-SIP) of Spores in Soils |
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Nicholson, Wayne L. Fedenko, Jeffrey Schuerger, Andrew C. |
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carbon-13 (13c) labeling of bacillus subtilis vegetative cells and spores: suitability for dna stable isotope probing (dna-sip) of spores in soils |
| title_auth |
Carbon-13 (13C) Labeling of Bacillus subtilis Vegetative Cells and Spores: Suitability for DNA Stable Isotope Probing (DNA-SIP) of Spores in Soils |
| abstract |
Abstract To test the suitability of DNA stable isotope probing (DNA-SIP) for characterizing bacterial spore populations in soils, the properties of Bacillus subtilis cells and spores intensely labeled with [13C]glucose were characterized. Spore germination, vegetative growth rates, and sporulation efficiency were indistinguishable on glucose versus [13C]glucose, as were spore wet heat and UV resistance. Unlabeled and 13C-labeled spores contained 1.0989 and 74.336 at.% 13C, and exhibited wet densities of 1.356 and 1.365 g/ml, respectively. Chromosomal DNAs containing 12C versus 13C were readily separated by their different buoyant densities in cesium chloride/ethidium bromide gradients. © Springer Science+Business Media, LLC 2009 |
| abstractGer |
Abstract To test the suitability of DNA stable isotope probing (DNA-SIP) for characterizing bacterial spore populations in soils, the properties of Bacillus subtilis cells and spores intensely labeled with [13C]glucose were characterized. Spore germination, vegetative growth rates, and sporulation efficiency were indistinguishable on glucose versus [13C]glucose, as were spore wet heat and UV resistance. Unlabeled and 13C-labeled spores contained 1.0989 and 74.336 at.% 13C, and exhibited wet densities of 1.356 and 1.365 g/ml, respectively. Chromosomal DNAs containing 12C versus 13C were readily separated by their different buoyant densities in cesium chloride/ethidium bromide gradients. © Springer Science+Business Media, LLC 2009 |
| abstract_unstemmed |
Abstract To test the suitability of DNA stable isotope probing (DNA-SIP) for characterizing bacterial spore populations in soils, the properties of Bacillus subtilis cells and spores intensely labeled with [13C]glucose were characterized. Spore germination, vegetative growth rates, and sporulation efficiency were indistinguishable on glucose versus [13C]glucose, as were spore wet heat and UV resistance. Unlabeled and 13C-labeled spores contained 1.0989 and 74.336 at.% 13C, and exhibited wet densities of 1.356 and 1.365 g/ml, respectively. Chromosomal DNAs containing 12C versus 13C were readily separated by their different buoyant densities in cesium chloride/ethidium bromide gradients. © Springer Science+Business Media, LLC 2009 |
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Carbon-13 (13C) Labeling of Bacillus subtilis Vegetative Cells and Spores: Suitability for DNA Stable Isotope Probing (DNA-SIP) of Spores in Soils |
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https://dx.doi.org/10.1007/s00284-009-9387-x |
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Fedenko, Jeffrey Schuerger, Andrew C. |
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10.1007/s00284-009-9387-x |
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2024-07-03T20:54:11.855Z |
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| score |
7.400522 |