Interplay Between Expression of Sulfur Assimilation Pathway Genes and $ Zn^{2+} $ and $ Pb^{2+} $ Stress in Acidithiobacillus ferrooxidans
Abstract We have previously demonstrated that in Acidithiobacillus ferrooxidans, resistance to the highly toxic divalent cation $ Cd^{2+} $ is mediated in part by the sulfur assimilation pathway (SAP) and enhanced intracellular concentrations of cysteine and glutathione(GSH) (Zheng et al., Extremoph...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Zheng, Chunli [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2016 |
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| Schlagwörter: |
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| Anmerkung: |
© Springer Science+Business Media New York 2016 |
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| Übergeordnetes Werk: |
Enthalten in: Current microbiology - New York, NY : Springer, 1978, 73(2016), 4 vom: 04. Juli, Seite 527-533 |
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| Übergeordnetes Werk: |
volume:73 ; year:2016 ; number:4 ; day:04 ; month:07 ; pages:527-533 |
| Links: |
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| DOI / URN: |
10.1007/s00284-016-1083-z |
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| Katalog-ID: |
SPR003685772 |
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| 245 | 1 | 0 | |a Interplay Between Expression of Sulfur Assimilation Pathway Genes and $ Zn^{2+} $ and $ Pb^{2+} $ Stress in Acidithiobacillus ferrooxidans |
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| 520 | |a Abstract We have previously demonstrated that in Acidithiobacillus ferrooxidans, resistance to the highly toxic divalent cation $ Cd^{2+} $ is mediated in part by the sulfur assimilation pathway (SAP) and enhanced intracellular concentrations of cysteine and glutathione(GSH) (Zheng et al., Extremophiles 19:429–436, 2015). In this paper, we investigate the interplay between $ Zn^{2+} $ and $ Pb^{2+} $ resistances, SAP gene expression, and thiol-containing metabolite levels. Cells grown in the presence of 300 mM $ Zn^{2+} $ had enhanced activities of the following enzymes: adenosylphosphosulphate reductase (APR, 40-fold), serine acetyltransferase (SAT, 180-fold), and O-acetylserine (thiol) lyase (OAS-TL, 230-fold). We investigated the concentrations of mRNA transcripts of the genes encoding these enzymes in cells grown in the presence of 600 mM $ Zn^{2+} $: transcripts for 4 SAP genes—ATPS(ATP sulphurylase), APR, SiR(sulfite reductase), SAT, and OAS-TL—each showed a more than three-fold increase in concentration. At the metabolite level, concentrations of intracellular cysteine and glutathione (GSH) were nearly doubled. When cells were grown in the presence of 10 mM $ Pb^{2+} $, SAP gene transcript concentrations, cysteine, and GSH concentrations were all decreased, as were SAP enzyme activities. These results suggested that $ Zn^{2+} $ induced SAP pathway gene transcription, while $ Pb^{2+} $ inhibited SAP gene expression and enzyme activities compared to the pathway in most organisms. Because of the detoxification function of thiol pool, the results also suggested that the high resistance of A. ferrooxidans to $ Zn^{2+} $ may also be due to regulation of GSH and the cysteine synthesis pathway. | ||
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| 650 | 4 | |a Sulfite Reductase |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Cysteine Level |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Sulfur Assimilation Pathway |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Intracellular Cysteine |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Chen, Minjie |4 aut | |
| 700 | 1 | |a Wang, Dan |4 aut | |
| 700 | 1 | |a Zhang, Li |4 aut | |
| 700 | 1 | |a Wang, JianYing |4 aut | |
| 700 | 1 | |a Zhang, Xuefeng |4 aut | |
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10.1007/s00284-016-1083-z doi (DE-627)SPR003685772 (SPR)s00284-016-1083-z-e DE-627 ger DE-627 rakwb eng Zheng, Chunli verfasserin aut Interplay Between Expression of Sulfur Assimilation Pathway Genes and $ Zn^{2+} $ and $ Pb^{2+} $ Stress in Acidithiobacillus ferrooxidans 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer Science+Business Media New York 2016 Abstract We have previously demonstrated that in Acidithiobacillus ferrooxidans, resistance to the highly toxic divalent cation $ Cd^{2+} $ is mediated in part by the sulfur assimilation pathway (SAP) and enhanced intracellular concentrations of cysteine and glutathione(GSH) (Zheng et al., Extremophiles 19:429–436, 2015). In this paper, we investigate the interplay between $ Zn^{2+} $ and $ Pb^{2+} $ resistances, SAP gene expression, and thiol-containing metabolite levels. Cells grown in the presence of 300 mM $ Zn^{2+} $ had enhanced activities of the following enzymes: adenosylphosphosulphate reductase (APR, 40-fold), serine acetyltransferase (SAT, 180-fold), and O-acetylserine (thiol) lyase (OAS-TL, 230-fold). We investigated the concentrations of mRNA transcripts of the genes encoding these enzymes in cells grown in the presence of 600 mM $ Zn^{2+} $: transcripts for 4 SAP genes—ATPS(ATP sulphurylase), APR, SiR(sulfite reductase), SAT, and OAS-TL—each showed a more than three-fold increase in concentration. At the metabolite level, concentrations of intracellular cysteine and glutathione (GSH) were nearly doubled. When cells were grown in the presence of 10 mM $ Pb^{2+} $, SAP gene transcript concentrations, cysteine, and GSH concentrations were all decreased, as were SAP enzyme activities. These results suggested that $ Zn^{2+} $ induced SAP pathway gene transcription, while $ Pb^{2+} $ inhibited SAP gene expression and enzyme activities compared to the pathway in most organisms. Because of the detoxification function of thiol pool, the results also suggested that the high resistance of A. ferrooxidans to $ Zn^{2+} $ may also be due to regulation of GSH and the cysteine synthesis pathway. Acidithiobacillus Ferrooxidans (dpeaa)DE-He213 Sulfite Reductase (dpeaa)DE-He213 Cysteine Level (dpeaa)DE-He213 Sulfur Assimilation Pathway (dpeaa)DE-He213 Intracellular Cysteine (dpeaa)DE-He213 Chen, Minjie aut Wang, Dan aut Zhang, Li aut Wang, JianYing aut Zhang, Xuefeng aut Enthalten in Current microbiology New York, NY : Springer, 1978 73(2016), 4 vom: 04. Juli, Seite 527-533 (DE-627)253722160 (DE-600)1458987-4 1432-0991 nnns volume:73 year:2016 number:4 day:04 month:07 pages:527-533 https://dx.doi.org/10.1007/s00284-016-1083-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 73 2016 4 04 07 527-533 |
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10.1007/s00284-016-1083-z doi (DE-627)SPR003685772 (SPR)s00284-016-1083-z-e DE-627 ger DE-627 rakwb eng Zheng, Chunli verfasserin aut Interplay Between Expression of Sulfur Assimilation Pathway Genes and $ Zn^{2+} $ and $ Pb^{2+} $ Stress in Acidithiobacillus ferrooxidans 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer Science+Business Media New York 2016 Abstract We have previously demonstrated that in Acidithiobacillus ferrooxidans, resistance to the highly toxic divalent cation $ Cd^{2+} $ is mediated in part by the sulfur assimilation pathway (SAP) and enhanced intracellular concentrations of cysteine and glutathione(GSH) (Zheng et al., Extremophiles 19:429–436, 2015). In this paper, we investigate the interplay between $ Zn^{2+} $ and $ Pb^{2+} $ resistances, SAP gene expression, and thiol-containing metabolite levels. Cells grown in the presence of 300 mM $ Zn^{2+} $ had enhanced activities of the following enzymes: adenosylphosphosulphate reductase (APR, 40-fold), serine acetyltransferase (SAT, 180-fold), and O-acetylserine (thiol) lyase (OAS-TL, 230-fold). We investigated the concentrations of mRNA transcripts of the genes encoding these enzymes in cells grown in the presence of 600 mM $ Zn^{2+} $: transcripts for 4 SAP genes—ATPS(ATP sulphurylase), APR, SiR(sulfite reductase), SAT, and OAS-TL—each showed a more than three-fold increase in concentration. At the metabolite level, concentrations of intracellular cysteine and glutathione (GSH) were nearly doubled. When cells were grown in the presence of 10 mM $ Pb^{2+} $, SAP gene transcript concentrations, cysteine, and GSH concentrations were all decreased, as were SAP enzyme activities. These results suggested that $ Zn^{2+} $ induced SAP pathway gene transcription, while $ Pb^{2+} $ inhibited SAP gene expression and enzyme activities compared to the pathway in most organisms. Because of the detoxification function of thiol pool, the results also suggested that the high resistance of A. ferrooxidans to $ Zn^{2+} $ may also be due to regulation of GSH and the cysteine synthesis pathway. Acidithiobacillus Ferrooxidans (dpeaa)DE-He213 Sulfite Reductase (dpeaa)DE-He213 Cysteine Level (dpeaa)DE-He213 Sulfur Assimilation Pathway (dpeaa)DE-He213 Intracellular Cysteine (dpeaa)DE-He213 Chen, Minjie aut Wang, Dan aut Zhang, Li aut Wang, JianYing aut Zhang, Xuefeng aut Enthalten in Current microbiology New York, NY : Springer, 1978 73(2016), 4 vom: 04. Juli, Seite 527-533 (DE-627)253722160 (DE-600)1458987-4 1432-0991 nnns volume:73 year:2016 number:4 day:04 month:07 pages:527-533 https://dx.doi.org/10.1007/s00284-016-1083-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 73 2016 4 04 07 527-533 |
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10.1007/s00284-016-1083-z doi (DE-627)SPR003685772 (SPR)s00284-016-1083-z-e DE-627 ger DE-627 rakwb eng Zheng, Chunli verfasserin aut Interplay Between Expression of Sulfur Assimilation Pathway Genes and $ Zn^{2+} $ and $ Pb^{2+} $ Stress in Acidithiobacillus ferrooxidans 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer Science+Business Media New York 2016 Abstract We have previously demonstrated that in Acidithiobacillus ferrooxidans, resistance to the highly toxic divalent cation $ Cd^{2+} $ is mediated in part by the sulfur assimilation pathway (SAP) and enhanced intracellular concentrations of cysteine and glutathione(GSH) (Zheng et al., Extremophiles 19:429–436, 2015). In this paper, we investigate the interplay between $ Zn^{2+} $ and $ Pb^{2+} $ resistances, SAP gene expression, and thiol-containing metabolite levels. Cells grown in the presence of 300 mM $ Zn^{2+} $ had enhanced activities of the following enzymes: adenosylphosphosulphate reductase (APR, 40-fold), serine acetyltransferase (SAT, 180-fold), and O-acetylserine (thiol) lyase (OAS-TL, 230-fold). We investigated the concentrations of mRNA transcripts of the genes encoding these enzymes in cells grown in the presence of 600 mM $ Zn^{2+} $: transcripts for 4 SAP genes—ATPS(ATP sulphurylase), APR, SiR(sulfite reductase), SAT, and OAS-TL—each showed a more than three-fold increase in concentration. At the metabolite level, concentrations of intracellular cysteine and glutathione (GSH) were nearly doubled. When cells were grown in the presence of 10 mM $ Pb^{2+} $, SAP gene transcript concentrations, cysteine, and GSH concentrations were all decreased, as were SAP enzyme activities. These results suggested that $ Zn^{2+} $ induced SAP pathway gene transcription, while $ Pb^{2+} $ inhibited SAP gene expression and enzyme activities compared to the pathway in most organisms. Because of the detoxification function of thiol pool, the results also suggested that the high resistance of A. ferrooxidans to $ Zn^{2+} $ may also be due to regulation of GSH and the cysteine synthesis pathway. Acidithiobacillus Ferrooxidans (dpeaa)DE-He213 Sulfite Reductase (dpeaa)DE-He213 Cysteine Level (dpeaa)DE-He213 Sulfur Assimilation Pathway (dpeaa)DE-He213 Intracellular Cysteine (dpeaa)DE-He213 Chen, Minjie aut Wang, Dan aut Zhang, Li aut Wang, JianYing aut Zhang, Xuefeng aut Enthalten in Current microbiology New York, NY : Springer, 1978 73(2016), 4 vom: 04. Juli, Seite 527-533 (DE-627)253722160 (DE-600)1458987-4 1432-0991 nnns volume:73 year:2016 number:4 day:04 month:07 pages:527-533 https://dx.doi.org/10.1007/s00284-016-1083-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 73 2016 4 04 07 527-533 |
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10.1007/s00284-016-1083-z doi (DE-627)SPR003685772 (SPR)s00284-016-1083-z-e DE-627 ger DE-627 rakwb eng Zheng, Chunli verfasserin aut Interplay Between Expression of Sulfur Assimilation Pathway Genes and $ Zn^{2+} $ and $ Pb^{2+} $ Stress in Acidithiobacillus ferrooxidans 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer Science+Business Media New York 2016 Abstract We have previously demonstrated that in Acidithiobacillus ferrooxidans, resistance to the highly toxic divalent cation $ Cd^{2+} $ is mediated in part by the sulfur assimilation pathway (SAP) and enhanced intracellular concentrations of cysteine and glutathione(GSH) (Zheng et al., Extremophiles 19:429–436, 2015). In this paper, we investigate the interplay between $ Zn^{2+} $ and $ Pb^{2+} $ resistances, SAP gene expression, and thiol-containing metabolite levels. Cells grown in the presence of 300 mM $ Zn^{2+} $ had enhanced activities of the following enzymes: adenosylphosphosulphate reductase (APR, 40-fold), serine acetyltransferase (SAT, 180-fold), and O-acetylserine (thiol) lyase (OAS-TL, 230-fold). We investigated the concentrations of mRNA transcripts of the genes encoding these enzymes in cells grown in the presence of 600 mM $ Zn^{2+} $: transcripts for 4 SAP genes—ATPS(ATP sulphurylase), APR, SiR(sulfite reductase), SAT, and OAS-TL—each showed a more than three-fold increase in concentration. At the metabolite level, concentrations of intracellular cysteine and glutathione (GSH) were nearly doubled. When cells were grown in the presence of 10 mM $ Pb^{2+} $, SAP gene transcript concentrations, cysteine, and GSH concentrations were all decreased, as were SAP enzyme activities. These results suggested that $ Zn^{2+} $ induced SAP pathway gene transcription, while $ Pb^{2+} $ inhibited SAP gene expression and enzyme activities compared to the pathway in most organisms. Because of the detoxification function of thiol pool, the results also suggested that the high resistance of A. ferrooxidans to $ Zn^{2+} $ may also be due to regulation of GSH and the cysteine synthesis pathway. Acidithiobacillus Ferrooxidans (dpeaa)DE-He213 Sulfite Reductase (dpeaa)DE-He213 Cysteine Level (dpeaa)DE-He213 Sulfur Assimilation Pathway (dpeaa)DE-He213 Intracellular Cysteine (dpeaa)DE-He213 Chen, Minjie aut Wang, Dan aut Zhang, Li aut Wang, JianYing aut Zhang, Xuefeng aut Enthalten in Current microbiology New York, NY : Springer, 1978 73(2016), 4 vom: 04. Juli, Seite 527-533 (DE-627)253722160 (DE-600)1458987-4 1432-0991 nnns volume:73 year:2016 number:4 day:04 month:07 pages:527-533 https://dx.doi.org/10.1007/s00284-016-1083-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 73 2016 4 04 07 527-533 |
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10.1007/s00284-016-1083-z doi (DE-627)SPR003685772 (SPR)s00284-016-1083-z-e DE-627 ger DE-627 rakwb eng Zheng, Chunli verfasserin aut Interplay Between Expression of Sulfur Assimilation Pathway Genes and $ Zn^{2+} $ and $ Pb^{2+} $ Stress in Acidithiobacillus ferrooxidans 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer Science+Business Media New York 2016 Abstract We have previously demonstrated that in Acidithiobacillus ferrooxidans, resistance to the highly toxic divalent cation $ Cd^{2+} $ is mediated in part by the sulfur assimilation pathway (SAP) and enhanced intracellular concentrations of cysteine and glutathione(GSH) (Zheng et al., Extremophiles 19:429–436, 2015). In this paper, we investigate the interplay between $ Zn^{2+} $ and $ Pb^{2+} $ resistances, SAP gene expression, and thiol-containing metabolite levels. Cells grown in the presence of 300 mM $ Zn^{2+} $ had enhanced activities of the following enzymes: adenosylphosphosulphate reductase (APR, 40-fold), serine acetyltransferase (SAT, 180-fold), and O-acetylserine (thiol) lyase (OAS-TL, 230-fold). We investigated the concentrations of mRNA transcripts of the genes encoding these enzymes in cells grown in the presence of 600 mM $ Zn^{2+} $: transcripts for 4 SAP genes—ATPS(ATP sulphurylase), APR, SiR(sulfite reductase), SAT, and OAS-TL—each showed a more than three-fold increase in concentration. At the metabolite level, concentrations of intracellular cysteine and glutathione (GSH) were nearly doubled. When cells were grown in the presence of 10 mM $ Pb^{2+} $, SAP gene transcript concentrations, cysteine, and GSH concentrations were all decreased, as were SAP enzyme activities. These results suggested that $ Zn^{2+} $ induced SAP pathway gene transcription, while $ Pb^{2+} $ inhibited SAP gene expression and enzyme activities compared to the pathway in most organisms. Because of the detoxification function of thiol pool, the results also suggested that the high resistance of A. ferrooxidans to $ Zn^{2+} $ may also be due to regulation of GSH and the cysteine synthesis pathway. Acidithiobacillus Ferrooxidans (dpeaa)DE-He213 Sulfite Reductase (dpeaa)DE-He213 Cysteine Level (dpeaa)DE-He213 Sulfur Assimilation Pathway (dpeaa)DE-He213 Intracellular Cysteine (dpeaa)DE-He213 Chen, Minjie aut Wang, Dan aut Zhang, Li aut Wang, JianYing aut Zhang, Xuefeng aut Enthalten in Current microbiology New York, NY : Springer, 1978 73(2016), 4 vom: 04. Juli, Seite 527-533 (DE-627)253722160 (DE-600)1458987-4 1432-0991 nnns volume:73 year:2016 number:4 day:04 month:07 pages:527-533 https://dx.doi.org/10.1007/s00284-016-1083-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 73 2016 4 04 07 527-533 |
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Enthalten in Current microbiology 73(2016), 4 vom: 04. Juli, Seite 527-533 volume:73 year:2016 number:4 day:04 month:07 pages:527-533 |
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Acidithiobacillus Ferrooxidans Sulfite Reductase Cysteine Level Sulfur Assimilation Pathway Intracellular Cysteine |
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Zheng, Chunli @@aut@@ Chen, Minjie @@aut@@ Wang, Dan @@aut@@ Zhang, Li @@aut@@ Wang, JianYing @@aut@@ Zhang, Xuefeng @@aut@@ |
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In this paper, we investigate the interplay between $ Zn^{2+} $ and $ Pb^{2+} $ resistances, SAP gene expression, and thiol-containing metabolite levels. Cells grown in the presence of 300 mM $ Zn^{2+} $ had enhanced activities of the following enzymes: adenosylphosphosulphate reductase (APR, 40-fold), serine acetyltransferase (SAT, 180-fold), and O-acetylserine (thiol) lyase (OAS-TL, 230-fold). We investigated the concentrations of mRNA transcripts of the genes encoding these enzymes in cells grown in the presence of 600 mM $ Zn^{2+} $: transcripts for 4 SAP genes—ATPS(ATP sulphurylase), APR, SiR(sulfite reductase), SAT, and OAS-TL—each showed a more than three-fold increase in concentration. At the metabolite level, concentrations of intracellular cysteine and glutathione (GSH) were nearly doubled. When cells were grown in the presence of 10 mM $ Pb^{2+} $, SAP gene transcript concentrations, cysteine, and GSH concentrations were all decreased, as were SAP enzyme activities. These results suggested that $ Zn^{2+} $ induced SAP pathway gene transcription, while $ Pb^{2+} $ inhibited SAP gene expression and enzyme activities compared to the pathway in most organisms. Because of the detoxification function of thiol pool, the results also suggested that the high resistance of A. ferrooxidans to $ Zn^{2+} $ may also be due to regulation of GSH and the cysteine synthesis pathway.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Acidithiobacillus Ferrooxidans</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Sulfite Reductase</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Cysteine Level</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Sulfur Assimilation Pathway</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Intracellular Cysteine</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Chen, Minjie</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Wang, Dan</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Zhang, Li</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Wang, JianYing</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Zhang, Xuefeng</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Current microbiology</subfield><subfield code="d">New York, NY : Springer, 1978</subfield><subfield code="g">73(2016), 4 vom: 04. 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Zheng, Chunli |
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Zheng, Chunli misc Acidithiobacillus Ferrooxidans misc Sulfite Reductase misc Cysteine Level misc Sulfur Assimilation Pathway misc Intracellular Cysteine Interplay Between Expression of Sulfur Assimilation Pathway Genes and $ Zn^{2+} $ and $ Pb^{2+} $ Stress in Acidithiobacillus ferrooxidans |
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Interplay Between Expression of Sulfur Assimilation Pathway Genes and $ Zn^{2+} $ and $ Pb^{2+} $ Stress in Acidithiobacillus ferrooxidans Acidithiobacillus Ferrooxidans (dpeaa)DE-He213 Sulfite Reductase (dpeaa)DE-He213 Cysteine Level (dpeaa)DE-He213 Sulfur Assimilation Pathway (dpeaa)DE-He213 Intracellular Cysteine (dpeaa)DE-He213 |
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misc Acidithiobacillus Ferrooxidans misc Sulfite Reductase misc Cysteine Level misc Sulfur Assimilation Pathway misc Intracellular Cysteine |
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Interplay Between Expression of Sulfur Assimilation Pathway Genes and $ Zn^{2+} $ and $ Pb^{2+} $ Stress in Acidithiobacillus ferrooxidans |
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Interplay Between Expression of Sulfur Assimilation Pathway Genes and $ Zn^{2+} $ and $ Pb^{2+} $ Stress in Acidithiobacillus ferrooxidans |
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Zheng, Chunli Chen, Minjie Wang, Dan Zhang, Li Wang, JianYing Zhang, Xuefeng |
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interplay between expression of sulfur assimilation pathway genes and $ zn^{2+} $ and $ pb^{2+} $ stress in acidithiobacillus ferrooxidans |
| title_auth |
Interplay Between Expression of Sulfur Assimilation Pathway Genes and $ Zn^{2+} $ and $ Pb^{2+} $ Stress in Acidithiobacillus ferrooxidans |
| abstract |
Abstract We have previously demonstrated that in Acidithiobacillus ferrooxidans, resistance to the highly toxic divalent cation $ Cd^{2+} $ is mediated in part by the sulfur assimilation pathway (SAP) and enhanced intracellular concentrations of cysteine and glutathione(GSH) (Zheng et al., Extremophiles 19:429–436, 2015). In this paper, we investigate the interplay between $ Zn^{2+} $ and $ Pb^{2+} $ resistances, SAP gene expression, and thiol-containing metabolite levels. Cells grown in the presence of 300 mM $ Zn^{2+} $ had enhanced activities of the following enzymes: adenosylphosphosulphate reductase (APR, 40-fold), serine acetyltransferase (SAT, 180-fold), and O-acetylserine (thiol) lyase (OAS-TL, 230-fold). We investigated the concentrations of mRNA transcripts of the genes encoding these enzymes in cells grown in the presence of 600 mM $ Zn^{2+} $: transcripts for 4 SAP genes—ATPS(ATP sulphurylase), APR, SiR(sulfite reductase), SAT, and OAS-TL—each showed a more than three-fold increase in concentration. At the metabolite level, concentrations of intracellular cysteine and glutathione (GSH) were nearly doubled. When cells were grown in the presence of 10 mM $ Pb^{2+} $, SAP gene transcript concentrations, cysteine, and GSH concentrations were all decreased, as were SAP enzyme activities. These results suggested that $ Zn^{2+} $ induced SAP pathway gene transcription, while $ Pb^{2+} $ inhibited SAP gene expression and enzyme activities compared to the pathway in most organisms. Because of the detoxification function of thiol pool, the results also suggested that the high resistance of A. ferrooxidans to $ Zn^{2+} $ may also be due to regulation of GSH and the cysteine synthesis pathway. © Springer Science+Business Media New York 2016 |
| abstractGer |
Abstract We have previously demonstrated that in Acidithiobacillus ferrooxidans, resistance to the highly toxic divalent cation $ Cd^{2+} $ is mediated in part by the sulfur assimilation pathway (SAP) and enhanced intracellular concentrations of cysteine and glutathione(GSH) (Zheng et al., Extremophiles 19:429–436, 2015). In this paper, we investigate the interplay between $ Zn^{2+} $ and $ Pb^{2+} $ resistances, SAP gene expression, and thiol-containing metabolite levels. Cells grown in the presence of 300 mM $ Zn^{2+} $ had enhanced activities of the following enzymes: adenosylphosphosulphate reductase (APR, 40-fold), serine acetyltransferase (SAT, 180-fold), and O-acetylserine (thiol) lyase (OAS-TL, 230-fold). We investigated the concentrations of mRNA transcripts of the genes encoding these enzymes in cells grown in the presence of 600 mM $ Zn^{2+} $: transcripts for 4 SAP genes—ATPS(ATP sulphurylase), APR, SiR(sulfite reductase), SAT, and OAS-TL—each showed a more than three-fold increase in concentration. At the metabolite level, concentrations of intracellular cysteine and glutathione (GSH) were nearly doubled. When cells were grown in the presence of 10 mM $ Pb^{2+} $, SAP gene transcript concentrations, cysteine, and GSH concentrations were all decreased, as were SAP enzyme activities. These results suggested that $ Zn^{2+} $ induced SAP pathway gene transcription, while $ Pb^{2+} $ inhibited SAP gene expression and enzyme activities compared to the pathway in most organisms. Because of the detoxification function of thiol pool, the results also suggested that the high resistance of A. ferrooxidans to $ Zn^{2+} $ may also be due to regulation of GSH and the cysteine synthesis pathway. © Springer Science+Business Media New York 2016 |
| abstract_unstemmed |
Abstract We have previously demonstrated that in Acidithiobacillus ferrooxidans, resistance to the highly toxic divalent cation $ Cd^{2+} $ is mediated in part by the sulfur assimilation pathway (SAP) and enhanced intracellular concentrations of cysteine and glutathione(GSH) (Zheng et al., Extremophiles 19:429–436, 2015). In this paper, we investigate the interplay between $ Zn^{2+} $ and $ Pb^{2+} $ resistances, SAP gene expression, and thiol-containing metabolite levels. Cells grown in the presence of 300 mM $ Zn^{2+} $ had enhanced activities of the following enzymes: adenosylphosphosulphate reductase (APR, 40-fold), serine acetyltransferase (SAT, 180-fold), and O-acetylserine (thiol) lyase (OAS-TL, 230-fold). We investigated the concentrations of mRNA transcripts of the genes encoding these enzymes in cells grown in the presence of 600 mM $ Zn^{2+} $: transcripts for 4 SAP genes—ATPS(ATP sulphurylase), APR, SiR(sulfite reductase), SAT, and OAS-TL—each showed a more than three-fold increase in concentration. At the metabolite level, concentrations of intracellular cysteine and glutathione (GSH) were nearly doubled. When cells were grown in the presence of 10 mM $ Pb^{2+} $, SAP gene transcript concentrations, cysteine, and GSH concentrations were all decreased, as were SAP enzyme activities. These results suggested that $ Zn^{2+} $ induced SAP pathway gene transcription, while $ Pb^{2+} $ inhibited SAP gene expression and enzyme activities compared to the pathway in most organisms. Because of the detoxification function of thiol pool, the results also suggested that the high resistance of A. ferrooxidans to $ Zn^{2+} $ may also be due to regulation of GSH and the cysteine synthesis pathway. © Springer Science+Business Media New York 2016 |
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4 |
| title_short |
Interplay Between Expression of Sulfur Assimilation Pathway Genes and $ Zn^{2+} $ and $ Pb^{2+} $ Stress in Acidithiobacillus ferrooxidans |
| url |
https://dx.doi.org/10.1007/s00284-016-1083-z |
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Chen, Minjie Wang, Dan Zhang, Li Wang, JianYing Zhang, Xuefeng |
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Chen, Minjie Wang, Dan Zhang, Li Wang, JianYing Zhang, Xuefeng |
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| doi_str |
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2024-07-03T21:01:24.505Z |
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| score |
7.3978605 |