Functional identification and regulation of the PtDrl02 gene promoter from triploid white poplar

Abstract The PtDrl02 gene belongs to the TIR-NBS gene family in triploid white poplar (Populus tomentosa × P. bolleana) × P. tomentosa. Its expression pattern displays tissue-specificity, and the transcript level can be induced by wounding, methyl jasmonate (MeJA), and salicylic acid (SA). To unders...
Ausführliche Beschreibung

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Autor*in:	Zheng, Huiquan [verfasserIn] Lin, Shanzhi Zhang, Qian Lei, Yang Hou, Lu Zhang, Zhiyi

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2010

Schlagwörter:	TIR-NBS -element Untranslated region Transcription factor

Anmerkung:	© Springer-Verlag 2010

Übergeordnetes Werk:	Enthalten in: Plant cell reports - Berlin : Springer, 1981, 29(2010), 5 vom: 24. Feb., Seite 449-460
Übergeordnetes Werk:	volume:29 ; year:2010 ; number:5 ; day:24 ; month:02 ; pages:449-460

Links:	Volltext

DOI / URN:	10.1007/s00299-010-0834-8

Katalog-ID:	SPR003898482

Internformat


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100	1		\|a Zheng, Huiquan \|e verfasserin \|4 aut
245	1	0	\|a Functional identification and regulation of the PtDrl02 gene promoter from triploid white poplar
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520			\|a Abstract The PtDrl02 gene belongs to the TIR-NBS gene family in triploid white poplar (Populus tomentosa × P. bolleana) × P. tomentosa. Its expression pattern displays tissue-specificity, and the transcript level can be induced by wounding, methyl jasmonate (MeJA), and salicylic acid (SA). To understand the regulatory mechanism controlling PtDrl02 gene expression, we functionally characterized the PtDrl02 promoter region. Using the β-glucuronidase as a reporter, we found that the PtDrl02 promoter directed gene expression mainly in the aerial parts of the plants and was confined to the cortex tissues of leaf veins, petioles, stems, and stem piths, showing a typical tissue-specific expression pattern. Deletion analysis revealed two positive regulatory regions (−985 to −669 and −669 to −467) responsible for the basal activity of the PtDrl02 promoter. Impressively, the sequence from –669 to –467 was shown to contain cis-element (s) responding to wounding and MeJA, while the promoter region between –244 and 0 could individually display wounding-responsiveness, and the fragment from –467 to –244 was required for SA- and NaCl-inducible expression of the PtDrl02 promoter. Additionally, it was found that the –985 to –669 sequence was the ABA-responding promoter fragment. These results suggested that the PtDrl02 promoter was modulated by multiple cis-regulatory elements in distinct and complex patterns to regulate PtDrl02 gene expression. Our study also suggested that the PtDrl02 gene 5′ untranslated region, as well as a Populus WRKY transcription factor, PtWRKY1, was involved in the regulation of PtDrl02 promoter activities.
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700	1		\|a Lin, Shanzhi \|4 aut
700	1		\|a Zhang, Qian \|4 aut
700	1		\|a Lei, Yang \|4 aut
700	1		\|a Hou, Lu \|4 aut
700	1		\|a Zhang, Zhiyi \|4 aut
773	0	8	\|i Enthalten in \|t Plant cell reports \|d Berlin : Springer, 1981 \|g 29(2010), 5 vom: 24. Feb., Seite 449-460 \|w (DE-627)254630367 \|w (DE-600)1462082-0 \|x 1432-203X \|7 nnns
773	1	8	\|g volume:29 \|g year:2010 \|g number:5 \|g day:24 \|g month:02 \|g pages:449-460
856	4	0	\|u https://dx.doi.org/10.1007/s00299-010-0834-8 \|z lizenzpflichtig \|3 Volltext
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912			\|a GBV_ILN_24
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912			\|a GBV_ILN_32
912			\|a GBV_ILN_39
912			\|a GBV_ILN_40
912			\|a GBV_ILN_60
912			\|a GBV_ILN_62
912			\|a GBV_ILN_63
912			\|a GBV_ILN_69
912			\|a GBV_ILN_70
912			\|a GBV_ILN_73
912			\|a GBV_ILN_74
912			\|a GBV_ILN_90
912			\|a GBV_ILN_95
912			\|a GBV_ILN_100
912			\|a GBV_ILN_101
912			\|a GBV_ILN_105
912			\|a GBV_ILN_110
912			\|a GBV_ILN_120
912			\|a GBV_ILN_138
912			\|a GBV_ILN_150
912			\|a GBV_ILN_151
912			\|a GBV_ILN_152
912			\|a GBV_ILN_161
912			\|a GBV_ILN_170
912			\|a GBV_ILN_171
912			\|a GBV_ILN_187
912			\|a GBV_ILN_213
912			\|a GBV_ILN_224
912			\|a GBV_ILN_230
912			\|a GBV_ILN_250
912			\|a GBV_ILN_267
912			\|a GBV_ILN_281
912			\|a GBV_ILN_285
912			\|a GBV_ILN_293
912			\|a GBV_ILN_370
912			\|a GBV_ILN_602
912			\|a GBV_ILN_636
912			\|a GBV_ILN_647
912			\|a GBV_ILN_702
912			\|a GBV_ILN_2001
912			\|a GBV_ILN_2003
912			\|a GBV_ILN_2004
912			\|a GBV_ILN_2005
912			\|a GBV_ILN_2006
912			\|a GBV_ILN_2007
912			\|a GBV_ILN_2008
912			\|a GBV_ILN_2009
912			\|a GBV_ILN_2010
912			\|a GBV_ILN_2011
912			\|a GBV_ILN_2014
912			\|a GBV_ILN_2015
912			\|a GBV_ILN_2020
912			\|a GBV_ILN_2021
912			\|a GBV_ILN_2025
912			\|a GBV_ILN_2026
912			\|a GBV_ILN_2027
912			\|a GBV_ILN_2031
912			\|a GBV_ILN_2034
912			\|a GBV_ILN_2037
912			\|a GBV_ILN_2038
912			\|a GBV_ILN_2039
912			\|a GBV_ILN_2044
912			\|a GBV_ILN_2048
912			\|a GBV_ILN_2049
912			\|a GBV_ILN_2050
912			\|a GBV_ILN_2055
912			\|a GBV_ILN_2057
912			\|a GBV_ILN_2059
912			\|a GBV_ILN_2061
912			\|a GBV_ILN_2064
912			\|a GBV_ILN_2065
912			\|a GBV_ILN_2068
912			\|a GBV_ILN_2070
912			\|a GBV_ILN_2086
912			\|a GBV_ILN_2088
912			\|a GBV_ILN_2093
912			\|a GBV_ILN_2106
912			\|a GBV_ILN_2107
912			\|a GBV_ILN_2108
912			\|a GBV_ILN_2110
912			\|a GBV_ILN_2111
912			\|a GBV_ILN_2112
912			\|a GBV_ILN_2113
912			\|a GBV_ILN_2116
912			\|a GBV_ILN_2118
912			\|a GBV_ILN_2119
912			\|a GBV_ILN_2122
912			\|a GBV_ILN_2129
912			\|a GBV_ILN_2143
912			\|a GBV_ILN_2144
912			\|a GBV_ILN_2147
912			\|a GBV_ILN_2148
912			\|a GBV_ILN_2152
912			\|a GBV_ILN_2153
912			\|a GBV_ILN_2188
912			\|a GBV_ILN_2190
912			\|a GBV_ILN_2232
912			\|a GBV_ILN_2336
912			\|a GBV_ILN_2446
912			\|a GBV_ILN_2470
912			\|a GBV_ILN_2472
912			\|a GBV_ILN_2507
912			\|a GBV_ILN_2522
912			\|a GBV_ILN_2548
912			\|a GBV_ILN_4012
912			\|a GBV_ILN_4035
912			\|a GBV_ILN_4037
912			\|a GBV_ILN_4046
912			\|a GBV_ILN_4112
912			\|a GBV_ILN_4125
912			\|a GBV_ILN_4126
912			\|a GBV_ILN_4242
912			\|a GBV_ILN_4246
912			\|a GBV_ILN_4249
912			\|a GBV_ILN_4251
912			\|a GBV_ILN_4305
912			\|a GBV_ILN_4306
912			\|a GBV_ILN_4307
912			\|a GBV_ILN_4313
912			\|a GBV_ILN_4322
912			\|a GBV_ILN_4323
912			\|a GBV_ILN_4324
912			\|a GBV_ILN_4325
912			\|a GBV_ILN_4326
912			\|a GBV_ILN_4328
912			\|a GBV_ILN_4333
912			\|a GBV_ILN_4334
912			\|a GBV_ILN_4335
912			\|a GBV_ILN_4336
912			\|a GBV_ILN_4338
912			\|a GBV_ILN_4393
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952			\|d 29 \|j 2010 \|e 5 \|b 24 \|c 02 \|h 449-460

Indexfelder

author_variant	h z hz s l sl q z qz y l yl l h lh z z zz
matchkey_str	article:1432203X:2010----::ucinldniiainnrgltootetr0gnpooef
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publishDate	2010
allfields	10.1007/s00299-010-0834-8 doi (DE-627)SPR003898482 (SPR)s00299-010-0834-8-e DE-627 ger DE-627 rakwb eng Zheng, Huiquan verfasserin aut Functional identification and regulation of the PtDrl02 gene promoter from triploid white poplar 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag 2010 Abstract The PtDrl02 gene belongs to the TIR-NBS gene family in triploid white poplar (Populus tomentosa × P. bolleana) × P. tomentosa. Its expression pattern displays tissue-specificity, and the transcript level can be induced by wounding, methyl jasmonate (MeJA), and salicylic acid (SA). To understand the regulatory mechanism controlling PtDrl02 gene expression, we functionally characterized the PtDrl02 promoter region. Using the β-glucuronidase as a reporter, we found that the PtDrl02 promoter directed gene expression mainly in the aerial parts of the plants and was confined to the cortex tissues of leaf veins, petioles, stems, and stem piths, showing a typical tissue-specific expression pattern. Deletion analysis revealed two positive regulatory regions (−985 to −669 and −669 to −467) responsible for the basal activity of the PtDrl02 promoter. Impressively, the sequence from –669 to –467 was shown to contain cis-element (s) responding to wounding and MeJA, while the promoter region between –244 and 0 could individually display wounding-responsiveness, and the fragment from –467 to –244 was required for SA- and NaCl-inducible expression of the PtDrl02 promoter. Additionally, it was found that the –985 to –669 sequence was the ABA-responding promoter fragment. These results suggested that the PtDrl02 promoter was modulated by multiple cis-regulatory elements in distinct and complex patterns to regulate PtDrl02 gene expression. Our study also suggested that the PtDrl02 gene 5′ untranslated region, as well as a Populus WRKY transcription factor, PtWRKY1, was involved in the regulation of PtDrl02 promoter activities. TIR-NBS (dpeaa)DE-He213 -element (dpeaa)DE-He213 Untranslated region (dpeaa)DE-He213 Transcription factor (dpeaa)DE-He213 Lin, Shanzhi aut Zhang, Qian aut Lei, Yang aut Hou, Lu aut Zhang, Zhiyi aut Enthalten in Plant cell reports Berlin : Springer, 1981 29(2010), 5 vom: 24. Feb., Seite 449-460 (DE-627)254630367 (DE-600)1462082-0 1432-203X nnns volume:29 year:2010 number:5 day:24 month:02 pages:449-460 https://dx.doi.org/10.1007/s00299-010-0834-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 29 2010 5 24 02 449-460
spelling	10.1007/s00299-010-0834-8 doi (DE-627)SPR003898482 (SPR)s00299-010-0834-8-e DE-627 ger DE-627 rakwb eng Zheng, Huiquan verfasserin aut Functional identification and regulation of the PtDrl02 gene promoter from triploid white poplar 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag 2010 Abstract The PtDrl02 gene belongs to the TIR-NBS gene family in triploid white poplar (Populus tomentosa × P. bolleana) × P. tomentosa. Its expression pattern displays tissue-specificity, and the transcript level can be induced by wounding, methyl jasmonate (MeJA), and salicylic acid (SA). To understand the regulatory mechanism controlling PtDrl02 gene expression, we functionally characterized the PtDrl02 promoter region. Using the β-glucuronidase as a reporter, we found that the PtDrl02 promoter directed gene expression mainly in the aerial parts of the plants and was confined to the cortex tissues of leaf veins, petioles, stems, and stem piths, showing a typical tissue-specific expression pattern. Deletion analysis revealed two positive regulatory regions (−985 to −669 and −669 to −467) responsible for the basal activity of the PtDrl02 promoter. Impressively, the sequence from –669 to –467 was shown to contain cis-element (s) responding to wounding and MeJA, while the promoter region between –244 and 0 could individually display wounding-responsiveness, and the fragment from –467 to –244 was required for SA- and NaCl-inducible expression of the PtDrl02 promoter. Additionally, it was found that the –985 to –669 sequence was the ABA-responding promoter fragment. These results suggested that the PtDrl02 promoter was modulated by multiple cis-regulatory elements in distinct and complex patterns to regulate PtDrl02 gene expression. Our study also suggested that the PtDrl02 gene 5′ untranslated region, as well as a Populus WRKY transcription factor, PtWRKY1, was involved in the regulation of PtDrl02 promoter activities. TIR-NBS (dpeaa)DE-He213 -element (dpeaa)DE-He213 Untranslated region (dpeaa)DE-He213 Transcription factor (dpeaa)DE-He213 Lin, Shanzhi aut Zhang, Qian aut Lei, Yang aut Hou, Lu aut Zhang, Zhiyi aut Enthalten in Plant cell reports Berlin : Springer, 1981 29(2010), 5 vom: 24. Feb., Seite 449-460 (DE-627)254630367 (DE-600)1462082-0 1432-203X nnns volume:29 year:2010 number:5 day:24 month:02 pages:449-460 https://dx.doi.org/10.1007/s00299-010-0834-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 29 2010 5 24 02 449-460
allfields_unstemmed	10.1007/s00299-010-0834-8 doi (DE-627)SPR003898482 (SPR)s00299-010-0834-8-e DE-627 ger DE-627 rakwb eng Zheng, Huiquan verfasserin aut Functional identification and regulation of the PtDrl02 gene promoter from triploid white poplar 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag 2010 Abstract The PtDrl02 gene belongs to the TIR-NBS gene family in triploid white poplar (Populus tomentosa × P. bolleana) × P. tomentosa. Its expression pattern displays tissue-specificity, and the transcript level can be induced by wounding, methyl jasmonate (MeJA), and salicylic acid (SA). To understand the regulatory mechanism controlling PtDrl02 gene expression, we functionally characterized the PtDrl02 promoter region. Using the β-glucuronidase as a reporter, we found that the PtDrl02 promoter directed gene expression mainly in the aerial parts of the plants and was confined to the cortex tissues of leaf veins, petioles, stems, and stem piths, showing a typical tissue-specific expression pattern. Deletion analysis revealed two positive regulatory regions (−985 to −669 and −669 to −467) responsible for the basal activity of the PtDrl02 promoter. Impressively, the sequence from –669 to –467 was shown to contain cis-element (s) responding to wounding and MeJA, while the promoter region between –244 and 0 could individually display wounding-responsiveness, and the fragment from –467 to –244 was required for SA- and NaCl-inducible expression of the PtDrl02 promoter. Additionally, it was found that the –985 to –669 sequence was the ABA-responding promoter fragment. These results suggested that the PtDrl02 promoter was modulated by multiple cis-regulatory elements in distinct and complex patterns to regulate PtDrl02 gene expression. Our study also suggested that the PtDrl02 gene 5′ untranslated region, as well as a Populus WRKY transcription factor, PtWRKY1, was involved in the regulation of PtDrl02 promoter activities. TIR-NBS (dpeaa)DE-He213 -element (dpeaa)DE-He213 Untranslated region (dpeaa)DE-He213 Transcription factor (dpeaa)DE-He213 Lin, Shanzhi aut Zhang, Qian aut Lei, Yang aut Hou, Lu aut Zhang, Zhiyi aut Enthalten in Plant cell reports Berlin : Springer, 1981 29(2010), 5 vom: 24. Feb., Seite 449-460 (DE-627)254630367 (DE-600)1462082-0 1432-203X nnns volume:29 year:2010 number:5 day:24 month:02 pages:449-460 https://dx.doi.org/10.1007/s00299-010-0834-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 29 2010 5 24 02 449-460
allfieldsGer	10.1007/s00299-010-0834-8 doi (DE-627)SPR003898482 (SPR)s00299-010-0834-8-e DE-627 ger DE-627 rakwb eng Zheng, Huiquan verfasserin aut Functional identification and regulation of the PtDrl02 gene promoter from triploid white poplar 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag 2010 Abstract The PtDrl02 gene belongs to the TIR-NBS gene family in triploid white poplar (Populus tomentosa × P. bolleana) × P. tomentosa. Its expression pattern displays tissue-specificity, and the transcript level can be induced by wounding, methyl jasmonate (MeJA), and salicylic acid (SA). To understand the regulatory mechanism controlling PtDrl02 gene expression, we functionally characterized the PtDrl02 promoter region. Using the β-glucuronidase as a reporter, we found that the PtDrl02 promoter directed gene expression mainly in the aerial parts of the plants and was confined to the cortex tissues of leaf veins, petioles, stems, and stem piths, showing a typical tissue-specific expression pattern. Deletion analysis revealed two positive regulatory regions (−985 to −669 and −669 to −467) responsible for the basal activity of the PtDrl02 promoter. Impressively, the sequence from –669 to –467 was shown to contain cis-element (s) responding to wounding and MeJA, while the promoter region between –244 and 0 could individually display wounding-responsiveness, and the fragment from –467 to –244 was required for SA- and NaCl-inducible expression of the PtDrl02 promoter. Additionally, it was found that the –985 to –669 sequence was the ABA-responding promoter fragment. These results suggested that the PtDrl02 promoter was modulated by multiple cis-regulatory elements in distinct and complex patterns to regulate PtDrl02 gene expression. Our study also suggested that the PtDrl02 gene 5′ untranslated region, as well as a Populus WRKY transcription factor, PtWRKY1, was involved in the regulation of PtDrl02 promoter activities. TIR-NBS (dpeaa)DE-He213 -element (dpeaa)DE-He213 Untranslated region (dpeaa)DE-He213 Transcription factor (dpeaa)DE-He213 Lin, Shanzhi aut Zhang, Qian aut Lei, Yang aut Hou, Lu aut Zhang, Zhiyi aut Enthalten in Plant cell reports Berlin : Springer, 1981 29(2010), 5 vom: 24. Feb., Seite 449-460 (DE-627)254630367 (DE-600)1462082-0 1432-203X nnns volume:29 year:2010 number:5 day:24 month:02 pages:449-460 https://dx.doi.org/10.1007/s00299-010-0834-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 29 2010 5 24 02 449-460
allfieldsSound	10.1007/s00299-010-0834-8 doi (DE-627)SPR003898482 (SPR)s00299-010-0834-8-e DE-627 ger DE-627 rakwb eng Zheng, Huiquan verfasserin aut Functional identification and regulation of the PtDrl02 gene promoter from triploid white poplar 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag 2010 Abstract The PtDrl02 gene belongs to the TIR-NBS gene family in triploid white poplar (Populus tomentosa × P. bolleana) × P. tomentosa. Its expression pattern displays tissue-specificity, and the transcript level can be induced by wounding, methyl jasmonate (MeJA), and salicylic acid (SA). To understand the regulatory mechanism controlling PtDrl02 gene expression, we functionally characterized the PtDrl02 promoter region. Using the β-glucuronidase as a reporter, we found that the PtDrl02 promoter directed gene expression mainly in the aerial parts of the plants and was confined to the cortex tissues of leaf veins, petioles, stems, and stem piths, showing a typical tissue-specific expression pattern. Deletion analysis revealed two positive regulatory regions (−985 to −669 and −669 to −467) responsible for the basal activity of the PtDrl02 promoter. Impressively, the sequence from –669 to –467 was shown to contain cis-element (s) responding to wounding and MeJA, while the promoter region between –244 and 0 could individually display wounding-responsiveness, and the fragment from –467 to –244 was required for SA- and NaCl-inducible expression of the PtDrl02 promoter. Additionally, it was found that the –985 to –669 sequence was the ABA-responding promoter fragment. These results suggested that the PtDrl02 promoter was modulated by multiple cis-regulatory elements in distinct and complex patterns to regulate PtDrl02 gene expression. Our study also suggested that the PtDrl02 gene 5′ untranslated region, as well as a Populus WRKY transcription factor, PtWRKY1, was involved in the regulation of PtDrl02 promoter activities. TIR-NBS (dpeaa)DE-He213 -element (dpeaa)DE-He213 Untranslated region (dpeaa)DE-He213 Transcription factor (dpeaa)DE-He213 Lin, Shanzhi aut Zhang, Qian aut Lei, Yang aut Hou, Lu aut Zhang, Zhiyi aut Enthalten in Plant cell reports Berlin : Springer, 1981 29(2010), 5 vom: 24. Feb., Seite 449-460 (DE-627)254630367 (DE-600)1462082-0 1432-203X nnns volume:29 year:2010 number:5 day:24 month:02 pages:449-460 https://dx.doi.org/10.1007/s00299-010-0834-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 29 2010 5 24 02 449-460
language	English
source	Enthalten in Plant cell reports 29(2010), 5 vom: 24. Feb., Seite 449-460 volume:29 year:2010 number:5 day:24 month:02 pages:449-460
sourceStr	Enthalten in Plant cell reports 29(2010), 5 vom: 24. Feb., Seite 449-460 volume:29 year:2010 number:5 day:24 month:02 pages:449-460
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	TIR-NBS -element Untranslated region Transcription factor
isfreeaccess_bool	false
container_title	Plant cell reports
authorswithroles_txt_mv	Zheng, Huiquan @@aut@@ Lin, Shanzhi @@aut@@ Zhang, Qian @@aut@@ Lei, Yang @@aut@@ Hou, Lu @@aut@@ Zhang, Zhiyi @@aut@@
publishDateDaySort_date	2010-02-24T00:00:00Z
hierarchy_top_id	254630367
id	SPR003898482
language_de	englisch
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author	Zheng, Huiquan
spellingShingle	Zheng, Huiquan misc TIR-NBS misc -element misc Untranslated region misc Transcription factor Functional identification and regulation of the PtDrl02 gene promoter from triploid white poplar
authorStr	Zheng, Huiquan
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topic_title	Functional identification and regulation of the PtDrl02 gene promoter from triploid white poplar TIR-NBS (dpeaa)DE-He213 -element (dpeaa)DE-He213 Untranslated region (dpeaa)DE-He213 Transcription factor (dpeaa)DE-He213
topic	misc TIR-NBS misc -element misc Untranslated region misc Transcription factor
topic_unstemmed	misc TIR-NBS misc -element misc Untranslated region misc Transcription factor
topic_browse	misc TIR-NBS misc -element misc Untranslated region misc Transcription factor
format_facet	Elektronische Aufsätze Aufsätze Elektronische Ressource
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title	Functional identification and regulation of the PtDrl02 gene promoter from triploid white poplar
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title_full	Functional identification and regulation of the PtDrl02 gene promoter from triploid white poplar
author_sort	Zheng, Huiquan
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author_browse	Zheng, Huiquan Lin, Shanzhi Zhang, Qian Lei, Yang Hou, Lu Zhang, Zhiyi
container_volume	29
format_se	Elektronische Aufsätze
author-letter	Zheng, Huiquan
doi_str_mv	10.1007/s00299-010-0834-8
title_sort	functional identification and regulation of the ptdrl02 gene promoter from triploid white poplar
title_auth	Functional identification and regulation of the PtDrl02 gene promoter from triploid white poplar
abstract	Abstract The PtDrl02 gene belongs to the TIR-NBS gene family in triploid white poplar (Populus tomentosa × P. bolleana) × P. tomentosa. Its expression pattern displays tissue-specificity, and the transcript level can be induced by wounding, methyl jasmonate (MeJA), and salicylic acid (SA). To understand the regulatory mechanism controlling PtDrl02 gene expression, we functionally characterized the PtDrl02 promoter region. Using the β-glucuronidase as a reporter, we found that the PtDrl02 promoter directed gene expression mainly in the aerial parts of the plants and was confined to the cortex tissues of leaf veins, petioles, stems, and stem piths, showing a typical tissue-specific expression pattern. Deletion analysis revealed two positive regulatory regions (−985 to −669 and −669 to −467) responsible for the basal activity of the PtDrl02 promoter. Impressively, the sequence from –669 to –467 was shown to contain cis-element (s) responding to wounding and MeJA, while the promoter region between –244 and 0 could individually display wounding-responsiveness, and the fragment from –467 to –244 was required for SA- and NaCl-inducible expression of the PtDrl02 promoter. Additionally, it was found that the –985 to –669 sequence was the ABA-responding promoter fragment. These results suggested that the PtDrl02 promoter was modulated by multiple cis-regulatory elements in distinct and complex patterns to regulate PtDrl02 gene expression. Our study also suggested that the PtDrl02 gene 5′ untranslated region, as well as a Populus WRKY transcription factor, PtWRKY1, was involved in the regulation of PtDrl02 promoter activities. © Springer-Verlag 2010
abstractGer	Abstract The PtDrl02 gene belongs to the TIR-NBS gene family in triploid white poplar (Populus tomentosa × P. bolleana) × P. tomentosa. Its expression pattern displays tissue-specificity, and the transcript level can be induced by wounding, methyl jasmonate (MeJA), and salicylic acid (SA). To understand the regulatory mechanism controlling PtDrl02 gene expression, we functionally characterized the PtDrl02 promoter region. Using the β-glucuronidase as a reporter, we found that the PtDrl02 promoter directed gene expression mainly in the aerial parts of the plants and was confined to the cortex tissues of leaf veins, petioles, stems, and stem piths, showing a typical tissue-specific expression pattern. Deletion analysis revealed two positive regulatory regions (−985 to −669 and −669 to −467) responsible for the basal activity of the PtDrl02 promoter. Impressively, the sequence from –669 to –467 was shown to contain cis-element (s) responding to wounding and MeJA, while the promoter region between –244 and 0 could individually display wounding-responsiveness, and the fragment from –467 to –244 was required for SA- and NaCl-inducible expression of the PtDrl02 promoter. Additionally, it was found that the –985 to –669 sequence was the ABA-responding promoter fragment. These results suggested that the PtDrl02 promoter was modulated by multiple cis-regulatory elements in distinct and complex patterns to regulate PtDrl02 gene expression. Our study also suggested that the PtDrl02 gene 5′ untranslated region, as well as a Populus WRKY transcription factor, PtWRKY1, was involved in the regulation of PtDrl02 promoter activities. © Springer-Verlag 2010
abstract_unstemmed	Abstract The PtDrl02 gene belongs to the TIR-NBS gene family in triploid white poplar (Populus tomentosa × P. bolleana) × P. tomentosa. Its expression pattern displays tissue-specificity, and the transcript level can be induced by wounding, methyl jasmonate (MeJA), and salicylic acid (SA). To understand the regulatory mechanism controlling PtDrl02 gene expression, we functionally characterized the PtDrl02 promoter region. Using the β-glucuronidase as a reporter, we found that the PtDrl02 promoter directed gene expression mainly in the aerial parts of the plants and was confined to the cortex tissues of leaf veins, petioles, stems, and stem piths, showing a typical tissue-specific expression pattern. Deletion analysis revealed two positive regulatory regions (−985 to −669 and −669 to −467) responsible for the basal activity of the PtDrl02 promoter. Impressively, the sequence from –669 to –467 was shown to contain cis-element (s) responding to wounding and MeJA, while the promoter region between –244 and 0 could individually display wounding-responsiveness, and the fragment from –467 to –244 was required for SA- and NaCl-inducible expression of the PtDrl02 promoter. Additionally, it was found that the –985 to –669 sequence was the ABA-responding promoter fragment. These results suggested that the PtDrl02 promoter was modulated by multiple cis-regulatory elements in distinct and complex patterns to regulate PtDrl02 gene expression. Our study also suggested that the PtDrl02 gene 5′ untranslated region, as well as a Populus WRKY transcription factor, PtWRKY1, was involved in the regulation of PtDrl02 promoter activities. © Springer-Verlag 2010
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container_issue	5
title_short	Functional identification and regulation of the PtDrl02 gene promoter from triploid white poplar
url	https://dx.doi.org/10.1007/s00299-010-0834-8
remote_bool	true
author2	Lin, Shanzhi Zhang, Qian Lei, Yang Hou, Lu Zhang, Zhiyi
author2Str	Lin, Shanzhi Zhang, Qian Lei, Yang Hou, Lu Zhang, Zhiyi
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doi_str	10.1007/s00299-010-0834-8
up_date	2024-07-03T22:23:11.362Z
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Its expression pattern displays tissue-specificity, and the transcript level can be induced by wounding, methyl jasmonate (MeJA), and salicylic acid (SA). To understand the regulatory mechanism controlling PtDrl02 gene expression, we functionally characterized the PtDrl02 promoter region. Using the β-glucuronidase as a reporter, we found that the PtDrl02 promoter directed gene expression mainly in the aerial parts of the plants and was confined to the cortex tissues of leaf veins, petioles, stems, and stem piths, showing a typical tissue-specific expression pattern. Deletion analysis revealed two positive regulatory regions (−985 to −669 and −669 to −467) responsible for the basal activity of the PtDrl02 promoter. 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Functional identification and regulation of the PtDrl02 gene promoter from triploid white poplar

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