Organ-specific alternatively spliced transcript isoforms of the sunflower SF21C gene
Abstract The sunflower sf21C gene is a member of a small plant gene family related to the human N-myc downstream-regulated gene family (NDRG) involved in stress and hormone responses, cell growth and differentiation. We have shown earlier that this sunflower gene is interrupted by nine introns and g...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Lazarescu, Eduard [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2010 |
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| Schlagwörter: |
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| Anmerkung: |
© Springer-Verlag 2010 |
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| Übergeordnetes Werk: |
Enthalten in: Plant cell reports - Berlin : Springer, 1981, 29(2010), 7 vom: 16. Apr., Seite 673-683 |
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| Übergeordnetes Werk: |
volume:29 ; year:2010 ; number:7 ; day:16 ; month:04 ; pages:673-683 |
| Links: |
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| DOI / URN: |
10.1007/s00299-010-0853-5 |
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| Katalog-ID: |
SPR003898709 |
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| 100 | 1 | |a Lazarescu, Eduard |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Organ-specific alternatively spliced transcript isoforms of the sunflower SF21C gene |
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| 520 | |a Abstract The sunflower sf21C gene is a member of a small plant gene family related to the human N-myc downstream-regulated gene family (NDRG) involved in stress and hormone responses, cell growth and differentiation. We have shown earlier that this sunflower gene is interrupted by nine introns and generates multiple mRNAs by alternative splicing of its primary transcript (Lazarescu et al. in Gene 374:77–86, 2006). In the present study, we have characterized 20 splice isoforms of this gene encoding 14 different protein variants. We show that these isoforms occur in distinct combinations in the various plant organs. Five identified variants were generated by splicing at novel sites, different from those classically recognized by U2- or U12-type spliceosomes. Twelve transcript variants contain premature termination codons (PTCs), which make them potential candidates for nonsense-mediated RNA degradation (NMD). Interestingly, all 12 transcripts are relatively abundant in at least one organ, suggesting that NMD is not a “default” mechanism for PTC-containing RNA transcripts generated by alternative splicing. These data indicate that alternative splicing of the sunflower sf21C primary transcript as well as the mRNA quality control mechanism by NMD are complex and regulated spatially and developmentally. | ||
| 650 | 4 | |a Splice variants |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Non-canonical splice sites |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Splicing pattern |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a RT-PCR |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a NMD |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Friedt, Wolfgang |4 aut | |
| 700 | 1 | |a Steinmetz, André |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Plant cell reports |d Berlin : Springer, 1981 |g 29(2010), 7 vom: 16. Apr., Seite 673-683 |w (DE-627)254630367 |w (DE-600)1462082-0 |x 1432-203X |7 nnns |
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| 912 | |a GBV_ILN_23 | ||
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| 912 | |a GBV_ILN_32 | ||
| 912 | |a GBV_ILN_39 | ||
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| 912 | |a GBV_ILN_60 | ||
| 912 | |a GBV_ILN_62 | ||
| 912 | |a GBV_ILN_63 | ||
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| 912 | |a GBV_ILN_70 | ||
| 912 | |a GBV_ILN_73 | ||
| 912 | |a GBV_ILN_74 | ||
| 912 | |a GBV_ILN_90 | ||
| 912 | |a GBV_ILN_95 | ||
| 912 | |a GBV_ILN_100 | ||
| 912 | |a GBV_ILN_101 | ||
| 912 | |a GBV_ILN_105 | ||
| 912 | |a GBV_ILN_110 | ||
| 912 | |a GBV_ILN_120 | ||
| 912 | |a GBV_ILN_138 | ||
| 912 | |a GBV_ILN_150 | ||
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| 912 | |a GBV_ILN_152 | ||
| 912 | |a GBV_ILN_161 | ||
| 912 | |a GBV_ILN_170 | ||
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| 912 | |a GBV_ILN_213 | ||
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| 912 | |a GBV_ILN_250 | ||
| 912 | |a GBV_ILN_267 | ||
| 912 | |a GBV_ILN_281 | ||
| 912 | |a GBV_ILN_285 | ||
| 912 | |a GBV_ILN_293 | ||
| 912 | |a GBV_ILN_370 | ||
| 912 | |a GBV_ILN_602 | ||
| 912 | |a GBV_ILN_636 | ||
| 912 | |a GBV_ILN_647 | ||
| 912 | |a GBV_ILN_702 | ||
| 912 | |a GBV_ILN_2001 | ||
| 912 | |a GBV_ILN_2003 | ||
| 912 | |a GBV_ILN_2004 | ||
| 912 | |a GBV_ILN_2005 | ||
| 912 | |a GBV_ILN_2006 | ||
| 912 | |a GBV_ILN_2007 | ||
| 912 | |a GBV_ILN_2008 | ||
| 912 | |a GBV_ILN_2009 | ||
| 912 | |a GBV_ILN_2010 | ||
| 912 | |a GBV_ILN_2011 | ||
| 912 | |a GBV_ILN_2014 | ||
| 912 | |a GBV_ILN_2015 | ||
| 912 | |a GBV_ILN_2020 | ||
| 912 | |a GBV_ILN_2021 | ||
| 912 | |a GBV_ILN_2025 | ||
| 912 | |a GBV_ILN_2026 | ||
| 912 | |a GBV_ILN_2027 | ||
| 912 | |a GBV_ILN_2031 | ||
| 912 | |a GBV_ILN_2034 | ||
| 912 | |a GBV_ILN_2037 | ||
| 912 | |a GBV_ILN_2038 | ||
| 912 | |a GBV_ILN_2039 | ||
| 912 | |a GBV_ILN_2044 | ||
| 912 | |a GBV_ILN_2048 | ||
| 912 | |a GBV_ILN_2049 | ||
| 912 | |a GBV_ILN_2050 | ||
| 912 | |a GBV_ILN_2055 | ||
| 912 | |a GBV_ILN_2057 | ||
| 912 | |a GBV_ILN_2059 | ||
| 912 | |a GBV_ILN_2061 | ||
| 912 | |a GBV_ILN_2064 | ||
| 912 | |a GBV_ILN_2065 | ||
| 912 | |a GBV_ILN_2068 | ||
| 912 | |a GBV_ILN_2070 | ||
| 912 | |a GBV_ILN_2086 | ||
| 912 | |a GBV_ILN_2088 | ||
| 912 | |a GBV_ILN_2093 | ||
| 912 | |a GBV_ILN_2106 | ||
| 912 | |a GBV_ILN_2107 | ||
| 912 | |a GBV_ILN_2108 | ||
| 912 | |a GBV_ILN_2110 | ||
| 912 | |a GBV_ILN_2111 | ||
| 912 | |a GBV_ILN_2112 | ||
| 912 | |a GBV_ILN_2113 | ||
| 912 | |a GBV_ILN_2116 | ||
| 912 | |a GBV_ILN_2118 | ||
| 912 | |a GBV_ILN_2119 | ||
| 912 | |a GBV_ILN_2122 | ||
| 912 | |a GBV_ILN_2129 | ||
| 912 | |a GBV_ILN_2143 | ||
| 912 | |a GBV_ILN_2144 | ||
| 912 | |a GBV_ILN_2147 | ||
| 912 | |a GBV_ILN_2148 | ||
| 912 | |a GBV_ILN_2152 | ||
| 912 | |a GBV_ILN_2153 | ||
| 912 | |a GBV_ILN_2188 | ||
| 912 | |a GBV_ILN_2190 | ||
| 912 | |a GBV_ILN_2232 | ||
| 912 | |a GBV_ILN_2336 | ||
| 912 | |a GBV_ILN_2446 | ||
| 912 | |a GBV_ILN_2470 | ||
| 912 | |a GBV_ILN_2472 | ||
| 912 | |a GBV_ILN_2507 | ||
| 912 | |a GBV_ILN_2522 | ||
| 912 | |a GBV_ILN_2548 | ||
| 912 | |a GBV_ILN_4012 | ||
| 912 | |a GBV_ILN_4035 | ||
| 912 | |a GBV_ILN_4037 | ||
| 912 | |a GBV_ILN_4046 | ||
| 912 | |a GBV_ILN_4112 | ||
| 912 | |a GBV_ILN_4125 | ||
| 912 | |a GBV_ILN_4126 | ||
| 912 | |a GBV_ILN_4242 | ||
| 912 | |a GBV_ILN_4246 | ||
| 912 | |a GBV_ILN_4249 | ||
| 912 | |a GBV_ILN_4251 | ||
| 912 | |a GBV_ILN_4305 | ||
| 912 | |a GBV_ILN_4306 | ||
| 912 | |a GBV_ILN_4307 | ||
| 912 | |a GBV_ILN_4313 | ||
| 912 | |a GBV_ILN_4322 | ||
| 912 | |a GBV_ILN_4323 | ||
| 912 | |a GBV_ILN_4324 | ||
| 912 | |a GBV_ILN_4325 | ||
| 912 | |a GBV_ILN_4326 | ||
| 912 | |a GBV_ILN_4328 | ||
| 912 | |a GBV_ILN_4333 | ||
| 912 | |a GBV_ILN_4334 | ||
| 912 | |a GBV_ILN_4335 | ||
| 912 | |a GBV_ILN_4336 | ||
| 912 | |a GBV_ILN_4338 | ||
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10.1007/s00299-010-0853-5 doi (DE-627)SPR003898709 (SPR)s00299-010-0853-5-e DE-627 ger DE-627 rakwb eng Lazarescu, Eduard verfasserin aut Organ-specific alternatively spliced transcript isoforms of the sunflower SF21C gene 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag 2010 Abstract The sunflower sf21C gene is a member of a small plant gene family related to the human N-myc downstream-regulated gene family (NDRG) involved in stress and hormone responses, cell growth and differentiation. We have shown earlier that this sunflower gene is interrupted by nine introns and generates multiple mRNAs by alternative splicing of its primary transcript (Lazarescu et al. in Gene 374:77–86, 2006). In the present study, we have characterized 20 splice isoforms of this gene encoding 14 different protein variants. We show that these isoforms occur in distinct combinations in the various plant organs. Five identified variants were generated by splicing at novel sites, different from those classically recognized by U2- or U12-type spliceosomes. Twelve transcript variants contain premature termination codons (PTCs), which make them potential candidates for nonsense-mediated RNA degradation (NMD). Interestingly, all 12 transcripts are relatively abundant in at least one organ, suggesting that NMD is not a “default” mechanism for PTC-containing RNA transcripts generated by alternative splicing. These data indicate that alternative splicing of the sunflower sf21C primary transcript as well as the mRNA quality control mechanism by NMD are complex and regulated spatially and developmentally. Splice variants (dpeaa)DE-He213 Non-canonical splice sites (dpeaa)DE-He213 Splicing pattern (dpeaa)DE-He213 RT-PCR (dpeaa)DE-He213 NMD (dpeaa)DE-He213 Friedt, Wolfgang aut Steinmetz, André aut Enthalten in Plant cell reports Berlin : Springer, 1981 29(2010), 7 vom: 16. Apr., Seite 673-683 (DE-627)254630367 (DE-600)1462082-0 1432-203X nnns volume:29 year:2010 number:7 day:16 month:04 pages:673-683 https://dx.doi.org/10.1007/s00299-010-0853-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 29 2010 7 16 04 673-683 |
| spelling |
10.1007/s00299-010-0853-5 doi (DE-627)SPR003898709 (SPR)s00299-010-0853-5-e DE-627 ger DE-627 rakwb eng Lazarescu, Eduard verfasserin aut Organ-specific alternatively spliced transcript isoforms of the sunflower SF21C gene 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag 2010 Abstract The sunflower sf21C gene is a member of a small plant gene family related to the human N-myc downstream-regulated gene family (NDRG) involved in stress and hormone responses, cell growth and differentiation. We have shown earlier that this sunflower gene is interrupted by nine introns and generates multiple mRNAs by alternative splicing of its primary transcript (Lazarescu et al. in Gene 374:77–86, 2006). In the present study, we have characterized 20 splice isoforms of this gene encoding 14 different protein variants. We show that these isoforms occur in distinct combinations in the various plant organs. Five identified variants were generated by splicing at novel sites, different from those classically recognized by U2- or U12-type spliceosomes. Twelve transcript variants contain premature termination codons (PTCs), which make them potential candidates for nonsense-mediated RNA degradation (NMD). Interestingly, all 12 transcripts are relatively abundant in at least one organ, suggesting that NMD is not a “default” mechanism for PTC-containing RNA transcripts generated by alternative splicing. These data indicate that alternative splicing of the sunflower sf21C primary transcript as well as the mRNA quality control mechanism by NMD are complex and regulated spatially and developmentally. Splice variants (dpeaa)DE-He213 Non-canonical splice sites (dpeaa)DE-He213 Splicing pattern (dpeaa)DE-He213 RT-PCR (dpeaa)DE-He213 NMD (dpeaa)DE-He213 Friedt, Wolfgang aut Steinmetz, André aut Enthalten in Plant cell reports Berlin : Springer, 1981 29(2010), 7 vom: 16. Apr., Seite 673-683 (DE-627)254630367 (DE-600)1462082-0 1432-203X nnns volume:29 year:2010 number:7 day:16 month:04 pages:673-683 https://dx.doi.org/10.1007/s00299-010-0853-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 29 2010 7 16 04 673-683 |
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10.1007/s00299-010-0853-5 doi (DE-627)SPR003898709 (SPR)s00299-010-0853-5-e DE-627 ger DE-627 rakwb eng Lazarescu, Eduard verfasserin aut Organ-specific alternatively spliced transcript isoforms of the sunflower SF21C gene 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag 2010 Abstract The sunflower sf21C gene is a member of a small plant gene family related to the human N-myc downstream-regulated gene family (NDRG) involved in stress and hormone responses, cell growth and differentiation. We have shown earlier that this sunflower gene is interrupted by nine introns and generates multiple mRNAs by alternative splicing of its primary transcript (Lazarescu et al. in Gene 374:77–86, 2006). In the present study, we have characterized 20 splice isoforms of this gene encoding 14 different protein variants. We show that these isoforms occur in distinct combinations in the various plant organs. Five identified variants were generated by splicing at novel sites, different from those classically recognized by U2- or U12-type spliceosomes. Twelve transcript variants contain premature termination codons (PTCs), which make them potential candidates for nonsense-mediated RNA degradation (NMD). Interestingly, all 12 transcripts are relatively abundant in at least one organ, suggesting that NMD is not a “default” mechanism for PTC-containing RNA transcripts generated by alternative splicing. These data indicate that alternative splicing of the sunflower sf21C primary transcript as well as the mRNA quality control mechanism by NMD are complex and regulated spatially and developmentally. Splice variants (dpeaa)DE-He213 Non-canonical splice sites (dpeaa)DE-He213 Splicing pattern (dpeaa)DE-He213 RT-PCR (dpeaa)DE-He213 NMD (dpeaa)DE-He213 Friedt, Wolfgang aut Steinmetz, André aut Enthalten in Plant cell reports Berlin : Springer, 1981 29(2010), 7 vom: 16. Apr., Seite 673-683 (DE-627)254630367 (DE-600)1462082-0 1432-203X nnns volume:29 year:2010 number:7 day:16 month:04 pages:673-683 https://dx.doi.org/10.1007/s00299-010-0853-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 29 2010 7 16 04 673-683 |
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10.1007/s00299-010-0853-5 doi (DE-627)SPR003898709 (SPR)s00299-010-0853-5-e DE-627 ger DE-627 rakwb eng Lazarescu, Eduard verfasserin aut Organ-specific alternatively spliced transcript isoforms of the sunflower SF21C gene 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag 2010 Abstract The sunflower sf21C gene is a member of a small plant gene family related to the human N-myc downstream-regulated gene family (NDRG) involved in stress and hormone responses, cell growth and differentiation. We have shown earlier that this sunflower gene is interrupted by nine introns and generates multiple mRNAs by alternative splicing of its primary transcript (Lazarescu et al. in Gene 374:77–86, 2006). In the present study, we have characterized 20 splice isoforms of this gene encoding 14 different protein variants. We show that these isoforms occur in distinct combinations in the various plant organs. Five identified variants were generated by splicing at novel sites, different from those classically recognized by U2- or U12-type spliceosomes. Twelve transcript variants contain premature termination codons (PTCs), which make them potential candidates for nonsense-mediated RNA degradation (NMD). Interestingly, all 12 transcripts are relatively abundant in at least one organ, suggesting that NMD is not a “default” mechanism for PTC-containing RNA transcripts generated by alternative splicing. These data indicate that alternative splicing of the sunflower sf21C primary transcript as well as the mRNA quality control mechanism by NMD are complex and regulated spatially and developmentally. Splice variants (dpeaa)DE-He213 Non-canonical splice sites (dpeaa)DE-He213 Splicing pattern (dpeaa)DE-He213 RT-PCR (dpeaa)DE-He213 NMD (dpeaa)DE-He213 Friedt, Wolfgang aut Steinmetz, André aut Enthalten in Plant cell reports Berlin : Springer, 1981 29(2010), 7 vom: 16. Apr., Seite 673-683 (DE-627)254630367 (DE-600)1462082-0 1432-203X nnns volume:29 year:2010 number:7 day:16 month:04 pages:673-683 https://dx.doi.org/10.1007/s00299-010-0853-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 29 2010 7 16 04 673-683 |
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10.1007/s00299-010-0853-5 doi (DE-627)SPR003898709 (SPR)s00299-010-0853-5-e DE-627 ger DE-627 rakwb eng Lazarescu, Eduard verfasserin aut Organ-specific alternatively spliced transcript isoforms of the sunflower SF21C gene 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag 2010 Abstract The sunflower sf21C gene is a member of a small plant gene family related to the human N-myc downstream-regulated gene family (NDRG) involved in stress and hormone responses, cell growth and differentiation. We have shown earlier that this sunflower gene is interrupted by nine introns and generates multiple mRNAs by alternative splicing of its primary transcript (Lazarescu et al. in Gene 374:77–86, 2006). In the present study, we have characterized 20 splice isoforms of this gene encoding 14 different protein variants. We show that these isoforms occur in distinct combinations in the various plant organs. Five identified variants were generated by splicing at novel sites, different from those classically recognized by U2- or U12-type spliceosomes. Twelve transcript variants contain premature termination codons (PTCs), which make them potential candidates for nonsense-mediated RNA degradation (NMD). Interestingly, all 12 transcripts are relatively abundant in at least one organ, suggesting that NMD is not a “default” mechanism for PTC-containing RNA transcripts generated by alternative splicing. These data indicate that alternative splicing of the sunflower sf21C primary transcript as well as the mRNA quality control mechanism by NMD are complex and regulated spatially and developmentally. Splice variants (dpeaa)DE-He213 Non-canonical splice sites (dpeaa)DE-He213 Splicing pattern (dpeaa)DE-He213 RT-PCR (dpeaa)DE-He213 NMD (dpeaa)DE-He213 Friedt, Wolfgang aut Steinmetz, André aut Enthalten in Plant cell reports Berlin : Springer, 1981 29(2010), 7 vom: 16. Apr., Seite 673-683 (DE-627)254630367 (DE-600)1462082-0 1432-203X nnns volume:29 year:2010 number:7 day:16 month:04 pages:673-683 https://dx.doi.org/10.1007/s00299-010-0853-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 29 2010 7 16 04 673-683 |
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We have shown earlier that this sunflower gene is interrupted by nine introns and generates multiple mRNAs by alternative splicing of its primary transcript (Lazarescu et al. in Gene 374:77–86, 2006). In the present study, we have characterized 20 splice isoforms of this gene encoding 14 different protein variants. We show that these isoforms occur in distinct combinations in the various plant organs. Five identified variants were generated by splicing at novel sites, different from those classically recognized by U2- or U12-type spliceosomes. Twelve transcript variants contain premature termination codons (PTCs), which make them potential candidates for nonsense-mediated RNA degradation (NMD). Interestingly, all 12 transcripts are relatively abundant in at least one organ, suggesting that NMD is not a “default” mechanism for PTC-containing RNA transcripts generated by alternative splicing. 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Lazarescu, Eduard |
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Organ-specific alternatively spliced transcript isoforms of the sunflower SF21C gene Splice variants (dpeaa)DE-He213 Non-canonical splice sites (dpeaa)DE-He213 Splicing pattern (dpeaa)DE-He213 RT-PCR (dpeaa)DE-He213 NMD (dpeaa)DE-He213 |
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organ-specific alternatively spliced transcript isoforms of the sunflower sf21c gene |
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Organ-specific alternatively spliced transcript isoforms of the sunflower SF21C gene |
| abstract |
Abstract The sunflower sf21C gene is a member of a small plant gene family related to the human N-myc downstream-regulated gene family (NDRG) involved in stress and hormone responses, cell growth and differentiation. We have shown earlier that this sunflower gene is interrupted by nine introns and generates multiple mRNAs by alternative splicing of its primary transcript (Lazarescu et al. in Gene 374:77–86, 2006). In the present study, we have characterized 20 splice isoforms of this gene encoding 14 different protein variants. We show that these isoforms occur in distinct combinations in the various plant organs. Five identified variants were generated by splicing at novel sites, different from those classically recognized by U2- or U12-type spliceosomes. Twelve transcript variants contain premature termination codons (PTCs), which make them potential candidates for nonsense-mediated RNA degradation (NMD). Interestingly, all 12 transcripts are relatively abundant in at least one organ, suggesting that NMD is not a “default” mechanism for PTC-containing RNA transcripts generated by alternative splicing. These data indicate that alternative splicing of the sunflower sf21C primary transcript as well as the mRNA quality control mechanism by NMD are complex and regulated spatially and developmentally. © Springer-Verlag 2010 |
| abstractGer |
Abstract The sunflower sf21C gene is a member of a small plant gene family related to the human N-myc downstream-regulated gene family (NDRG) involved in stress and hormone responses, cell growth and differentiation. We have shown earlier that this sunflower gene is interrupted by nine introns and generates multiple mRNAs by alternative splicing of its primary transcript (Lazarescu et al. in Gene 374:77–86, 2006). In the present study, we have characterized 20 splice isoforms of this gene encoding 14 different protein variants. We show that these isoforms occur in distinct combinations in the various plant organs. Five identified variants were generated by splicing at novel sites, different from those classically recognized by U2- or U12-type spliceosomes. Twelve transcript variants contain premature termination codons (PTCs), which make them potential candidates for nonsense-mediated RNA degradation (NMD). Interestingly, all 12 transcripts are relatively abundant in at least one organ, suggesting that NMD is not a “default” mechanism for PTC-containing RNA transcripts generated by alternative splicing. These data indicate that alternative splicing of the sunflower sf21C primary transcript as well as the mRNA quality control mechanism by NMD are complex and regulated spatially and developmentally. © Springer-Verlag 2010 |
| abstract_unstemmed |
Abstract The sunflower sf21C gene is a member of a small plant gene family related to the human N-myc downstream-regulated gene family (NDRG) involved in stress and hormone responses, cell growth and differentiation. We have shown earlier that this sunflower gene is interrupted by nine introns and generates multiple mRNAs by alternative splicing of its primary transcript (Lazarescu et al. in Gene 374:77–86, 2006). In the present study, we have characterized 20 splice isoforms of this gene encoding 14 different protein variants. We show that these isoforms occur in distinct combinations in the various plant organs. Five identified variants were generated by splicing at novel sites, different from those classically recognized by U2- or U12-type spliceosomes. Twelve transcript variants contain premature termination codons (PTCs), which make them potential candidates for nonsense-mediated RNA degradation (NMD). Interestingly, all 12 transcripts are relatively abundant in at least one organ, suggesting that NMD is not a “default” mechanism for PTC-containing RNA transcripts generated by alternative splicing. These data indicate that alternative splicing of the sunflower sf21C primary transcript as well as the mRNA quality control mechanism by NMD are complex and regulated spatially and developmentally. © Springer-Verlag 2010 |
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| title_short |
Organ-specific alternatively spliced transcript isoforms of the sunflower SF21C gene |
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https://dx.doi.org/10.1007/s00299-010-0853-5 |
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Friedt, Wolfgang Steinmetz, André |
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10.1007/s00299-010-0853-5 |
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2024-07-03T22:23:17.448Z |
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| score |
7.3998213 |