Value and limits of immunohistochemistry in differential diagnosis of clear cell primary brain tumors

Abstract Clear cell histology is the hallmark of oligodendroglioma (OG) but also characterizes clear cell ependymoma (CCE) and central neurocytoma (CN). Immunohistochemistry for glial and neuronal proteins may support differential diagnosis. We investigated systematically diagnostic value and limits...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Koperek, Oskar [verfasserIn] Gelpi, Ellen [verfasserIn] Birner, Peter [verfasserIn] Haberler, Christine [verfasserIn] Budka, Herbert [verfasserIn] Hainfellner, Johannes A. [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2004

Schlagwörter:	Clear cell ependymoma Oligodendroglioma Central neurocytoma Immunohistochemistry

Übergeordnetes Werk:	Enthalten in: Acta neuropathologica - Berlin : Springer, 1961, 108(2004), 1 vom: 23. Apr., Seite 24-30
Übergeordnetes Werk:	volume:108 ; year:2004 ; number:1 ; day:23 ; month:04 ; pages:24-30

Links:	Volltext

DOI / URN:	10.1007/s00401-004-0856-9

Katalog-ID:	SPR004971817

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245	1	0	\|a Value and limits of immunohistochemistry in differential diagnosis of clear cell primary brain tumors
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520			\|a Abstract Clear cell histology is the hallmark of oligodendroglioma (OG) but also characterizes clear cell ependymoma (CCE) and central neurocytoma (CN). Immunohistochemistry for glial and neuronal proteins may support differential diagnosis. We investigated systematically diagnostic value and limits of immunohistochemistry using representative tumor specimens (>1 cm in diameter) of well-defined OGs, CCEs, and CNs (n=10, respectively). Antibodies comprised anti-neuron specific nuclear protein (NEUN), anti-synaptophysin, anti-neuron-specific enolase, anti-microtubule-associated protein 2, anti-phosphorylated neurofilament protein, anti-non-phosphorylated neurofilament protein, anti-glial fibrillary acidic protein (GFAP), anti-S100 protein, anti-vimentin (VIM), and anti-epithelial membrane antigen (EMA). Among the panel of antibodies anti-NEUN, anti-VIM and anti-EMA proved most useful for differential diagnosis. Prominent (>90%) anti-NEUN immunolabeling of tumor cells clearly distinguished CNs from OGs and CCEs. Anti-VIM immunolabeling and a characteristic cytoplasmic dot-like anti-EMA immunoreactivity pattern of tumor cells were detectable only in OGs and CCEs. Furthermore, prominent anti-VIM immunoreactivity and anti-EMA cell membrane staining including ring-like staining pattern is characteristic for CCEs. Additionally, a widespread gliofibrillary and minigemistocytic cytoplasmic anti-GFAP immunostaining pattern is restricted to some OGs. Our data indicate that immunohistochemistry using anti-NEUN, anti-VIM, and anti-EMA on representative tumor specimens allows clear-cut distinction of CNs vs OGs and CCEs. Anti-VIM, anti-EMA, and anti-GFAP support differential diagnosis of OGs vs CCEs. Nevertheless, it is noted that due to focal expression of glial proteins in CNs and, conversely, of neuronal proteins in OGs and CCEs, immunohistochemistry is of limited value on small tumor specimens.
650		4	\|a Clear cell ependymoma \|7 (dpeaa)DE-He213
650		4	\|a Oligodendroglioma \|7 (dpeaa)DE-He213
650		4	\|a Central neurocytoma \|7 (dpeaa)DE-He213
650		4	\|a Immunohistochemistry \|7 (dpeaa)DE-He213
700	1		\|a Gelpi, Ellen \|e verfasserin \|4 aut
700	1		\|a Birner, Peter \|e verfasserin \|4 aut
700	1		\|a Haberler, Christine \|e verfasserin \|4 aut
700	1		\|a Budka, Herbert \|e verfasserin \|4 aut
700	1		\|a Hainfellner, Johannes A. \|e verfasserin \|4 aut
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912			\|a GBV_ILN_2048
912			\|a GBV_ILN_2049
912			\|a GBV_ILN_2050
912			\|a GBV_ILN_2055
912			\|a GBV_ILN_2057
912			\|a GBV_ILN_2059
912			\|a GBV_ILN_2061
912			\|a GBV_ILN_2064
912			\|a GBV_ILN_2065
912			\|a GBV_ILN_2068
912			\|a GBV_ILN_2070
912			\|a GBV_ILN_2086
912			\|a GBV_ILN_2088
912			\|a GBV_ILN_2093
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951			\|a AR
952			\|d 108 \|j 2004 \|e 1 \|b 23 \|c 04 \|h 24-30

Indexfelder

author_variant	o k ok e g eg p b pb c h ch h b hb j a h ja jah
matchkey_str	article:14320533:2004----::auadiisfmuoitceitynifrniligoiocer
hierarchy_sort_str	2004
bklnumber	44.90
publishDate	2004
allfields	10.1007/s00401-004-0856-9 doi (DE-627)SPR004971817 (SPR)s00401-004-0856-9-e DE-627 ger DE-627 rakwb eng 610 ASE 44.90 bkl Koperek, Oskar verfasserin aut Value and limits of immunohistochemistry in differential diagnosis of clear cell primary brain tumors 2004 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Clear cell histology is the hallmark of oligodendroglioma (OG) but also characterizes clear cell ependymoma (CCE) and central neurocytoma (CN). Immunohistochemistry for glial and neuronal proteins may support differential diagnosis. We investigated systematically diagnostic value and limits of immunohistochemistry using representative tumor specimens (>1 cm in diameter) of well-defined OGs, CCEs, and CNs (n=10, respectively). Antibodies comprised anti-neuron specific nuclear protein (NEUN), anti-synaptophysin, anti-neuron-specific enolase, anti-microtubule-associated protein 2, anti-phosphorylated neurofilament protein, anti-non-phosphorylated neurofilament protein, anti-glial fibrillary acidic protein (GFAP), anti-S100 protein, anti-vimentin (VIM), and anti-epithelial membrane antigen (EMA). Among the panel of antibodies anti-NEUN, anti-VIM and anti-EMA proved most useful for differential diagnosis. Prominent (>90%) anti-NEUN immunolabeling of tumor cells clearly distinguished CNs from OGs and CCEs. Anti-VIM immunolabeling and a characteristic cytoplasmic dot-like anti-EMA immunoreactivity pattern of tumor cells were detectable only in OGs and CCEs. Furthermore, prominent anti-VIM immunoreactivity and anti-EMA cell membrane staining including ring-like staining pattern is characteristic for CCEs. Additionally, a widespread gliofibrillary and minigemistocytic cytoplasmic anti-GFAP immunostaining pattern is restricted to some OGs. Our data indicate that immunohistochemistry using anti-NEUN, anti-VIM, and anti-EMA on representative tumor specimens allows clear-cut distinction of CNs vs OGs and CCEs. Anti-VIM, anti-EMA, and anti-GFAP support differential diagnosis of OGs vs CCEs. Nevertheless, it is noted that due to focal expression of glial proteins in CNs and, conversely, of neuronal proteins in OGs and CCEs, immunohistochemistry is of limited value on small tumor specimens. Clear cell ependymoma (dpeaa)DE-He213 Oligodendroglioma (dpeaa)DE-He213 Central neurocytoma (dpeaa)DE-He213 Immunohistochemistry (dpeaa)DE-He213 Gelpi, Ellen verfasserin aut Birner, Peter verfasserin aut Haberler, Christine verfasserin aut Budka, Herbert verfasserin aut Hainfellner, Johannes A. verfasserin aut Enthalten in Acta neuropathologica Berlin : Springer, 1961 108(2004), 1 vom: 23. Apr., Seite 24-30 (DE-627)253389666 (DE-600)1458410-4 1432-0533 nnns volume:108 year:2004 number:1 day:23 month:04 pages:24-30 https://dx.doi.org/10.1007/s00401-004-0856-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.90 ASE AR 108 2004 1 23 04 24-30
spelling	10.1007/s00401-004-0856-9 doi (DE-627)SPR004971817 (SPR)s00401-004-0856-9-e DE-627 ger DE-627 rakwb eng 610 ASE 44.90 bkl Koperek, Oskar verfasserin aut Value and limits of immunohistochemistry in differential diagnosis of clear cell primary brain tumors 2004 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Clear cell histology is the hallmark of oligodendroglioma (OG) but also characterizes clear cell ependymoma (CCE) and central neurocytoma (CN). Immunohistochemistry for glial and neuronal proteins may support differential diagnosis. We investigated systematically diagnostic value and limits of immunohistochemistry using representative tumor specimens (>1 cm in diameter) of well-defined OGs, CCEs, and CNs (n=10, respectively). Antibodies comprised anti-neuron specific nuclear protein (NEUN), anti-synaptophysin, anti-neuron-specific enolase, anti-microtubule-associated protein 2, anti-phosphorylated neurofilament protein, anti-non-phosphorylated neurofilament protein, anti-glial fibrillary acidic protein (GFAP), anti-S100 protein, anti-vimentin (VIM), and anti-epithelial membrane antigen (EMA). Among the panel of antibodies anti-NEUN, anti-VIM and anti-EMA proved most useful for differential diagnosis. Prominent (>90%) anti-NEUN immunolabeling of tumor cells clearly distinguished CNs from OGs and CCEs. Anti-VIM immunolabeling and a characteristic cytoplasmic dot-like anti-EMA immunoreactivity pattern of tumor cells were detectable only in OGs and CCEs. Furthermore, prominent anti-VIM immunoreactivity and anti-EMA cell membrane staining including ring-like staining pattern is characteristic for CCEs. Additionally, a widespread gliofibrillary and minigemistocytic cytoplasmic anti-GFAP immunostaining pattern is restricted to some OGs. Our data indicate that immunohistochemistry using anti-NEUN, anti-VIM, and anti-EMA on representative tumor specimens allows clear-cut distinction of CNs vs OGs and CCEs. Anti-VIM, anti-EMA, and anti-GFAP support differential diagnosis of OGs vs CCEs. Nevertheless, it is noted that due to focal expression of glial proteins in CNs and, conversely, of neuronal proteins in OGs and CCEs, immunohistochemistry is of limited value on small tumor specimens. Clear cell ependymoma (dpeaa)DE-He213 Oligodendroglioma (dpeaa)DE-He213 Central neurocytoma (dpeaa)DE-He213 Immunohistochemistry (dpeaa)DE-He213 Gelpi, Ellen verfasserin aut Birner, Peter verfasserin aut Haberler, Christine verfasserin aut Budka, Herbert verfasserin aut Hainfellner, Johannes A. verfasserin aut Enthalten in Acta neuropathologica Berlin : Springer, 1961 108(2004), 1 vom: 23. Apr., Seite 24-30 (DE-627)253389666 (DE-600)1458410-4 1432-0533 nnns volume:108 year:2004 number:1 day:23 month:04 pages:24-30 https://dx.doi.org/10.1007/s00401-004-0856-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.90 ASE AR 108 2004 1 23 04 24-30
allfields_unstemmed	10.1007/s00401-004-0856-9 doi (DE-627)SPR004971817 (SPR)s00401-004-0856-9-e DE-627 ger DE-627 rakwb eng 610 ASE 44.90 bkl Koperek, Oskar verfasserin aut Value and limits of immunohistochemistry in differential diagnosis of clear cell primary brain tumors 2004 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Clear cell histology is the hallmark of oligodendroglioma (OG) but also characterizes clear cell ependymoma (CCE) and central neurocytoma (CN). Immunohistochemistry for glial and neuronal proteins may support differential diagnosis. We investigated systematically diagnostic value and limits of immunohistochemistry using representative tumor specimens (>1 cm in diameter) of well-defined OGs, CCEs, and CNs (n=10, respectively). Antibodies comprised anti-neuron specific nuclear protein (NEUN), anti-synaptophysin, anti-neuron-specific enolase, anti-microtubule-associated protein 2, anti-phosphorylated neurofilament protein, anti-non-phosphorylated neurofilament protein, anti-glial fibrillary acidic protein (GFAP), anti-S100 protein, anti-vimentin (VIM), and anti-epithelial membrane antigen (EMA). Among the panel of antibodies anti-NEUN, anti-VIM and anti-EMA proved most useful for differential diagnosis. Prominent (>90%) anti-NEUN immunolabeling of tumor cells clearly distinguished CNs from OGs and CCEs. Anti-VIM immunolabeling and a characteristic cytoplasmic dot-like anti-EMA immunoreactivity pattern of tumor cells were detectable only in OGs and CCEs. Furthermore, prominent anti-VIM immunoreactivity and anti-EMA cell membrane staining including ring-like staining pattern is characteristic for CCEs. Additionally, a widespread gliofibrillary and minigemistocytic cytoplasmic anti-GFAP immunostaining pattern is restricted to some OGs. Our data indicate that immunohistochemistry using anti-NEUN, anti-VIM, and anti-EMA on representative tumor specimens allows clear-cut distinction of CNs vs OGs and CCEs. Anti-VIM, anti-EMA, and anti-GFAP support differential diagnosis of OGs vs CCEs. Nevertheless, it is noted that due to focal expression of glial proteins in CNs and, conversely, of neuronal proteins in OGs and CCEs, immunohistochemistry is of limited value on small tumor specimens. Clear cell ependymoma (dpeaa)DE-He213 Oligodendroglioma (dpeaa)DE-He213 Central neurocytoma (dpeaa)DE-He213 Immunohistochemistry (dpeaa)DE-He213 Gelpi, Ellen verfasserin aut Birner, Peter verfasserin aut Haberler, Christine verfasserin aut Budka, Herbert verfasserin aut Hainfellner, Johannes A. verfasserin aut Enthalten in Acta neuropathologica Berlin : Springer, 1961 108(2004), 1 vom: 23. Apr., Seite 24-30 (DE-627)253389666 (DE-600)1458410-4 1432-0533 nnns volume:108 year:2004 number:1 day:23 month:04 pages:24-30 https://dx.doi.org/10.1007/s00401-004-0856-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.90 ASE AR 108 2004 1 23 04 24-30
allfieldsGer	10.1007/s00401-004-0856-9 doi (DE-627)SPR004971817 (SPR)s00401-004-0856-9-e DE-627 ger DE-627 rakwb eng 610 ASE 44.90 bkl Koperek, Oskar verfasserin aut Value and limits of immunohistochemistry in differential diagnosis of clear cell primary brain tumors 2004 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Clear cell histology is the hallmark of oligodendroglioma (OG) but also characterizes clear cell ependymoma (CCE) and central neurocytoma (CN). Immunohistochemistry for glial and neuronal proteins may support differential diagnosis. We investigated systematically diagnostic value and limits of immunohistochemistry using representative tumor specimens (>1 cm in diameter) of well-defined OGs, CCEs, and CNs (n=10, respectively). Antibodies comprised anti-neuron specific nuclear protein (NEUN), anti-synaptophysin, anti-neuron-specific enolase, anti-microtubule-associated protein 2, anti-phosphorylated neurofilament protein, anti-non-phosphorylated neurofilament protein, anti-glial fibrillary acidic protein (GFAP), anti-S100 protein, anti-vimentin (VIM), and anti-epithelial membrane antigen (EMA). Among the panel of antibodies anti-NEUN, anti-VIM and anti-EMA proved most useful for differential diagnosis. Prominent (>90%) anti-NEUN immunolabeling of tumor cells clearly distinguished CNs from OGs and CCEs. Anti-VIM immunolabeling and a characteristic cytoplasmic dot-like anti-EMA immunoreactivity pattern of tumor cells were detectable only in OGs and CCEs. Furthermore, prominent anti-VIM immunoreactivity and anti-EMA cell membrane staining including ring-like staining pattern is characteristic for CCEs. Additionally, a widespread gliofibrillary and minigemistocytic cytoplasmic anti-GFAP immunostaining pattern is restricted to some OGs. Our data indicate that immunohistochemistry using anti-NEUN, anti-VIM, and anti-EMA on representative tumor specimens allows clear-cut distinction of CNs vs OGs and CCEs. Anti-VIM, anti-EMA, and anti-GFAP support differential diagnosis of OGs vs CCEs. Nevertheless, it is noted that due to focal expression of glial proteins in CNs and, conversely, of neuronal proteins in OGs and CCEs, immunohistochemistry is of limited value on small tumor specimens. Clear cell ependymoma (dpeaa)DE-He213 Oligodendroglioma (dpeaa)DE-He213 Central neurocytoma (dpeaa)DE-He213 Immunohistochemistry (dpeaa)DE-He213 Gelpi, Ellen verfasserin aut Birner, Peter verfasserin aut Haberler, Christine verfasserin aut Budka, Herbert verfasserin aut Hainfellner, Johannes A. verfasserin aut Enthalten in Acta neuropathologica Berlin : Springer, 1961 108(2004), 1 vom: 23. Apr., Seite 24-30 (DE-627)253389666 (DE-600)1458410-4 1432-0533 nnns volume:108 year:2004 number:1 day:23 month:04 pages:24-30 https://dx.doi.org/10.1007/s00401-004-0856-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.90 ASE AR 108 2004 1 23 04 24-30
allfieldsSound	10.1007/s00401-004-0856-9 doi (DE-627)SPR004971817 (SPR)s00401-004-0856-9-e DE-627 ger DE-627 rakwb eng 610 ASE 44.90 bkl Koperek, Oskar verfasserin aut Value and limits of immunohistochemistry in differential diagnosis of clear cell primary brain tumors 2004 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Clear cell histology is the hallmark of oligodendroglioma (OG) but also characterizes clear cell ependymoma (CCE) and central neurocytoma (CN). Immunohistochemistry for glial and neuronal proteins may support differential diagnosis. We investigated systematically diagnostic value and limits of immunohistochemistry using representative tumor specimens (>1 cm in diameter) of well-defined OGs, CCEs, and CNs (n=10, respectively). Antibodies comprised anti-neuron specific nuclear protein (NEUN), anti-synaptophysin, anti-neuron-specific enolase, anti-microtubule-associated protein 2, anti-phosphorylated neurofilament protein, anti-non-phosphorylated neurofilament protein, anti-glial fibrillary acidic protein (GFAP), anti-S100 protein, anti-vimentin (VIM), and anti-epithelial membrane antigen (EMA). Among the panel of antibodies anti-NEUN, anti-VIM and anti-EMA proved most useful for differential diagnosis. Prominent (>90%) anti-NEUN immunolabeling of tumor cells clearly distinguished CNs from OGs and CCEs. Anti-VIM immunolabeling and a characteristic cytoplasmic dot-like anti-EMA immunoreactivity pattern of tumor cells were detectable only in OGs and CCEs. Furthermore, prominent anti-VIM immunoreactivity and anti-EMA cell membrane staining including ring-like staining pattern is characteristic for CCEs. Additionally, a widespread gliofibrillary and minigemistocytic cytoplasmic anti-GFAP immunostaining pattern is restricted to some OGs. Our data indicate that immunohistochemistry using anti-NEUN, anti-VIM, and anti-EMA on representative tumor specimens allows clear-cut distinction of CNs vs OGs and CCEs. Anti-VIM, anti-EMA, and anti-GFAP support differential diagnosis of OGs vs CCEs. Nevertheless, it is noted that due to focal expression of glial proteins in CNs and, conversely, of neuronal proteins in OGs and CCEs, immunohistochemistry is of limited value on small tumor specimens. Clear cell ependymoma (dpeaa)DE-He213 Oligodendroglioma (dpeaa)DE-He213 Central neurocytoma (dpeaa)DE-He213 Immunohistochemistry (dpeaa)DE-He213 Gelpi, Ellen verfasserin aut Birner, Peter verfasserin aut Haberler, Christine verfasserin aut Budka, Herbert verfasserin aut Hainfellner, Johannes A. verfasserin aut Enthalten in Acta neuropathologica Berlin : Springer, 1961 108(2004), 1 vom: 23. Apr., Seite 24-30 (DE-627)253389666 (DE-600)1458410-4 1432-0533 nnns volume:108 year:2004 number:1 day:23 month:04 pages:24-30 https://dx.doi.org/10.1007/s00401-004-0856-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.90 ASE AR 108 2004 1 23 04 24-30
language	English
source	Enthalten in Acta neuropathologica 108(2004), 1 vom: 23. Apr., Seite 24-30 volume:108 year:2004 number:1 day:23 month:04 pages:24-30
sourceStr	Enthalten in Acta neuropathologica 108(2004), 1 vom: 23. Apr., Seite 24-30 volume:108 year:2004 number:1 day:23 month:04 pages:24-30
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Clear cell ependymoma Oligodendroglioma Central neurocytoma Immunohistochemistry
dewey-raw	610
isfreeaccess_bool	false
container_title	Acta neuropathologica
authorswithroles_txt_mv	Koperek, Oskar @@aut@@ Gelpi, Ellen @@aut@@ Birner, Peter @@aut@@ Haberler, Christine @@aut@@ Budka, Herbert @@aut@@ Hainfellner, Johannes A. @@aut@@
publishDateDaySort_date	2004-04-23T00:00:00Z
hierarchy_top_id	253389666
dewey-sort	3610
id	SPR004971817
language_de	englisch
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author	Koperek, Oskar
spellingShingle	Koperek, Oskar ddc 610 bkl 44.90 misc Clear cell ependymoma misc Oligodendroglioma misc Central neurocytoma misc Immunohistochemistry Value and limits of immunohistochemistry in differential diagnosis of clear cell primary brain tumors
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topic_title	610 ASE 44.90 bkl Value and limits of immunohistochemistry in differential diagnosis of clear cell primary brain tumors Clear cell ependymoma (dpeaa)DE-He213 Oligodendroglioma (dpeaa)DE-He213 Central neurocytoma (dpeaa)DE-He213 Immunohistochemistry (dpeaa)DE-He213
topic	ddc 610 bkl 44.90 misc Clear cell ependymoma misc Oligodendroglioma misc Central neurocytoma misc Immunohistochemistry
topic_unstemmed	ddc 610 bkl 44.90 misc Clear cell ependymoma misc Oligodendroglioma misc Central neurocytoma misc Immunohistochemistry
topic_browse	ddc 610 bkl 44.90 misc Clear cell ependymoma misc Oligodendroglioma misc Central neurocytoma misc Immunohistochemistry
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title	Value and limits of immunohistochemistry in differential diagnosis of clear cell primary brain tumors
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title_full	Value and limits of immunohistochemistry in differential diagnosis of clear cell primary brain tumors
author_sort	Koperek, Oskar
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author_browse	Koperek, Oskar Gelpi, Ellen Birner, Peter Haberler, Christine Budka, Herbert Hainfellner, Johannes A.
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title_sort	value and limits of immunohistochemistry in differential diagnosis of clear cell primary brain tumors
title_auth	Value and limits of immunohistochemistry in differential diagnosis of clear cell primary brain tumors
abstract	Abstract Clear cell histology is the hallmark of oligodendroglioma (OG) but also characterizes clear cell ependymoma (CCE) and central neurocytoma (CN). Immunohistochemistry for glial and neuronal proteins may support differential diagnosis. We investigated systematically diagnostic value and limits of immunohistochemistry using representative tumor specimens (>1 cm in diameter) of well-defined OGs, CCEs, and CNs (n=10, respectively). Antibodies comprised anti-neuron specific nuclear protein (NEUN), anti-synaptophysin, anti-neuron-specific enolase, anti-microtubule-associated protein 2, anti-phosphorylated neurofilament protein, anti-non-phosphorylated neurofilament protein, anti-glial fibrillary acidic protein (GFAP), anti-S100 protein, anti-vimentin (VIM), and anti-epithelial membrane antigen (EMA). Among the panel of antibodies anti-NEUN, anti-VIM and anti-EMA proved most useful for differential diagnosis. Prominent (>90%) anti-NEUN immunolabeling of tumor cells clearly distinguished CNs from OGs and CCEs. Anti-VIM immunolabeling and a characteristic cytoplasmic dot-like anti-EMA immunoreactivity pattern of tumor cells were detectable only in OGs and CCEs. Furthermore, prominent anti-VIM immunoreactivity and anti-EMA cell membrane staining including ring-like staining pattern is characteristic for CCEs. Additionally, a widespread gliofibrillary and minigemistocytic cytoplasmic anti-GFAP immunostaining pattern is restricted to some OGs. Our data indicate that immunohistochemistry using anti-NEUN, anti-VIM, and anti-EMA on representative tumor specimens allows clear-cut distinction of CNs vs OGs and CCEs. Anti-VIM, anti-EMA, and anti-GFAP support differential diagnosis of OGs vs CCEs. Nevertheless, it is noted that due to focal expression of glial proteins in CNs and, conversely, of neuronal proteins in OGs and CCEs, immunohistochemistry is of limited value on small tumor specimens.
abstractGer	Abstract Clear cell histology is the hallmark of oligodendroglioma (OG) but also characterizes clear cell ependymoma (CCE) and central neurocytoma (CN). Immunohistochemistry for glial and neuronal proteins may support differential diagnosis. We investigated systematically diagnostic value and limits of immunohistochemistry using representative tumor specimens (>1 cm in diameter) of well-defined OGs, CCEs, and CNs (n=10, respectively). Antibodies comprised anti-neuron specific nuclear protein (NEUN), anti-synaptophysin, anti-neuron-specific enolase, anti-microtubule-associated protein 2, anti-phosphorylated neurofilament protein, anti-non-phosphorylated neurofilament protein, anti-glial fibrillary acidic protein (GFAP), anti-S100 protein, anti-vimentin (VIM), and anti-epithelial membrane antigen (EMA). Among the panel of antibodies anti-NEUN, anti-VIM and anti-EMA proved most useful for differential diagnosis. Prominent (>90%) anti-NEUN immunolabeling of tumor cells clearly distinguished CNs from OGs and CCEs. Anti-VIM immunolabeling and a characteristic cytoplasmic dot-like anti-EMA immunoreactivity pattern of tumor cells were detectable only in OGs and CCEs. Furthermore, prominent anti-VIM immunoreactivity and anti-EMA cell membrane staining including ring-like staining pattern is characteristic for CCEs. Additionally, a widespread gliofibrillary and minigemistocytic cytoplasmic anti-GFAP immunostaining pattern is restricted to some OGs. Our data indicate that immunohistochemistry using anti-NEUN, anti-VIM, and anti-EMA on representative tumor specimens allows clear-cut distinction of CNs vs OGs and CCEs. Anti-VIM, anti-EMA, and anti-GFAP support differential diagnosis of OGs vs CCEs. Nevertheless, it is noted that due to focal expression of glial proteins in CNs and, conversely, of neuronal proteins in OGs and CCEs, immunohistochemistry is of limited value on small tumor specimens.
abstract_unstemmed	Abstract Clear cell histology is the hallmark of oligodendroglioma (OG) but also characterizes clear cell ependymoma (CCE) and central neurocytoma (CN). Immunohistochemistry for glial and neuronal proteins may support differential diagnosis. We investigated systematically diagnostic value and limits of immunohistochemistry using representative tumor specimens (>1 cm in diameter) of well-defined OGs, CCEs, and CNs (n=10, respectively). Antibodies comprised anti-neuron specific nuclear protein (NEUN), anti-synaptophysin, anti-neuron-specific enolase, anti-microtubule-associated protein 2, anti-phosphorylated neurofilament protein, anti-non-phosphorylated neurofilament protein, anti-glial fibrillary acidic protein (GFAP), anti-S100 protein, anti-vimentin (VIM), and anti-epithelial membrane antigen (EMA). Among the panel of antibodies anti-NEUN, anti-VIM and anti-EMA proved most useful for differential diagnosis. Prominent (>90%) anti-NEUN immunolabeling of tumor cells clearly distinguished CNs from OGs and CCEs. Anti-VIM immunolabeling and a characteristic cytoplasmic dot-like anti-EMA immunoreactivity pattern of tumor cells were detectable only in OGs and CCEs. Furthermore, prominent anti-VIM immunoreactivity and anti-EMA cell membrane staining including ring-like staining pattern is characteristic for CCEs. Additionally, a widespread gliofibrillary and minigemistocytic cytoplasmic anti-GFAP immunostaining pattern is restricted to some OGs. Our data indicate that immunohistochemistry using anti-NEUN, anti-VIM, and anti-EMA on representative tumor specimens allows clear-cut distinction of CNs vs OGs and CCEs. Anti-VIM, anti-EMA, and anti-GFAP support differential diagnosis of OGs vs CCEs. Nevertheless, it is noted that due to focal expression of glial proteins in CNs and, conversely, of neuronal proteins in OGs and CCEs, immunohistochemistry is of limited value on small tumor specimens.
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container_issue	1
title_short	Value and limits of immunohistochemistry in differential diagnosis of clear cell primary brain tumors
url	https://dx.doi.org/10.1007/s00401-004-0856-9
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author2	Gelpi, Ellen Birner, Peter Haberler, Christine Budka, Herbert Hainfellner, Johannes A.
author2Str	Gelpi, Ellen Birner, Peter Haberler, Christine Budka, Herbert Hainfellner, Johannes A.
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doi_str	10.1007/s00401-004-0856-9
up_date	2024-07-04T03:14:14.053Z
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Value and limits of immunohistochemistry in differential diagnosis of clear cell primary brain tumors

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