Association of hypoxia-inducible factor-1 expression with histology in epithelial ovarian tumors: a quantitative analysis of HIF-1
Background Hypoxia-inducible factor-1 (HIF-1) is an essential transcription factor that mediates cellular and systemic homeostatic responses to reduced oxygen availability in mammals. So far, using immunohistochemistry we have analyzed the association of HIF-1α expression with histological type amon...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Miyazawa, Masaki [verfasserIn] Yasuda, Masanori [verfasserIn] Fujita, Mariko [verfasserIn] Hirasawa, Takeshi [verfasserIn] Kajiwara, Hiroshi [verfasserIn] Hirabayashi, Kenichi [verfasserIn] Ogane, Naoki [verfasserIn] Shimizu, Michio [verfasserIn] Asanuma, Hideki [verfasserIn] Murakami, Masaru [verfasserIn] Takekoshi, Susumu [verfasserIn] Mikami, Mikio [verfasserIn] Osamura, R. Yoshiyuki [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2008 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Archives of gynecology and obstetrics - Berlin : Springer, 1870, 279(2008), 6 vom: 21. Okt., Seite 789-796 |
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| Übergeordnetes Werk: |
volume:279 ; year:2008 ; number:6 ; day:21 ; month:10 ; pages:789-796 |
| Links: |
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| DOI / URN: |
10.1007/s00404-008-0816-z |
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| Katalog-ID: |
SPR005098963 |
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| 100 | 1 | |a Miyazawa, Masaki |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Association of hypoxia-inducible factor-1 expression with histology in epithelial ovarian tumors: a quantitative analysis of HIF-1 |
| 264 | 1 | |c 2008 | |
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| 520 | |a Background Hypoxia-inducible factor-1 (HIF-1) is an essential transcription factor that mediates cellular and systemic homeostatic responses to reduced oxygen availability in mammals. So far, using immunohistochemistry we have analyzed the association of HIF-1α expression with histological type among epithelial ovarian tumors. In the present study, quantitative analyses of activated HIF-1 level in the nucleus and of accumulated HIF-1α level in the cytoplasm were performed to clarify whether or not the hypoxic state would be correlated to histology, malignancy, and tumor size in epithelial ovarian tumors. Method HIF-1 level in the nucleus was analyzed using DNA binding assay, and HIF-1α level in the cytoplasm was measured by ELISA for a total of 36 epithelial ovarian tumors as follows: 5 serous adenocarcinomas (SEAs), 7 clear cell adenocarcinomas (CLAs), 7 endometrioid adenocarcinomas (ENAs), 4 mucinous adenocarcinomas (MUAs), 2 mucinous borderline tumors (MBTs), and 11 mucinous adenomas. Results HIF-1 level (mg/ml) in the nucleus and HIF-1α level (mg/ml) in the cytoplasm were on average 0.116 and 0.178 for SEAs, 0.328 and 0.306 for CLAs, 0.171 and 0.305 for ENAs, 0.097 and 0.176 for MUAs, 0.224 and 0.180 for mucinous borderline tumors, 0.152 and 0.154 for mucinous adenomas. CLAs showed the highest levels for both of HIF-1 and HIF-1α, while MUAs showed the lowest levels of both. Mucinous adenomas were higher in HIF-1 than MUAs. Conclusion Hypoxic state was considered to be closely related to histological type of epithelial ovarian tumors, suggesting that CLAs may be most hypoxic. In the comparison of mucinous tumors, malignancies would not always become most hypoxic. Tumor size may not be strongly associated with hypoxic state. | ||
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| 650 | 4 | |a DNA binding HIF-1 activity |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Ovary |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Yasuda, Masanori |e verfasserin |4 aut | |
| 700 | 1 | |a Fujita, Mariko |e verfasserin |4 aut | |
| 700 | 1 | |a Hirasawa, Takeshi |e verfasserin |4 aut | |
| 700 | 1 | |a Kajiwara, Hiroshi |e verfasserin |4 aut | |
| 700 | 1 | |a Hirabayashi, Kenichi |e verfasserin |4 aut | |
| 700 | 1 | |a Ogane, Naoki |e verfasserin |4 aut | |
| 700 | 1 | |a Shimizu, Michio |e verfasserin |4 aut | |
| 700 | 1 | |a Asanuma, Hideki |e verfasserin |4 aut | |
| 700 | 1 | |a Murakami, Masaru |e verfasserin |4 aut | |
| 700 | 1 | |a Takekoshi, Susumu |e verfasserin |4 aut | |
| 700 | 1 | |a Mikami, Mikio |e verfasserin |4 aut | |
| 700 | 1 | |a Osamura, R. Yoshiyuki |e verfasserin |4 aut | |
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10.1007/s00404-008-0816-z doi (DE-627)SPR005098963 (SPR)s00404-008-0816-z-e DE-627 ger DE-627 rakwb eng 610 ASE 44.92 bkl Miyazawa, Masaki verfasserin aut Association of hypoxia-inducible factor-1 expression with histology in epithelial ovarian tumors: a quantitative analysis of HIF-1 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background Hypoxia-inducible factor-1 (HIF-1) is an essential transcription factor that mediates cellular and systemic homeostatic responses to reduced oxygen availability in mammals. So far, using immunohistochemistry we have analyzed the association of HIF-1α expression with histological type among epithelial ovarian tumors. In the present study, quantitative analyses of activated HIF-1 level in the nucleus and of accumulated HIF-1α level in the cytoplasm were performed to clarify whether or not the hypoxic state would be correlated to histology, malignancy, and tumor size in epithelial ovarian tumors. Method HIF-1 level in the nucleus was analyzed using DNA binding assay, and HIF-1α level in the cytoplasm was measured by ELISA for a total of 36 epithelial ovarian tumors as follows: 5 serous adenocarcinomas (SEAs), 7 clear cell adenocarcinomas (CLAs), 7 endometrioid adenocarcinomas (ENAs), 4 mucinous adenocarcinomas (MUAs), 2 mucinous borderline tumors (MBTs), and 11 mucinous adenomas. Results HIF-1 level (mg/ml) in the nucleus and HIF-1α level (mg/ml) in the cytoplasm were on average 0.116 and 0.178 for SEAs, 0.328 and 0.306 for CLAs, 0.171 and 0.305 for ENAs, 0.097 and 0.176 for MUAs, 0.224 and 0.180 for mucinous borderline tumors, 0.152 and 0.154 for mucinous adenomas. CLAs showed the highest levels for both of HIF-1 and HIF-1α, while MUAs showed the lowest levels of both. Mucinous adenomas were higher in HIF-1 than MUAs. Conclusion Hypoxic state was considered to be closely related to histological type of epithelial ovarian tumors, suggesting that CLAs may be most hypoxic. In the comparison of mucinous tumors, malignancies would not always become most hypoxic. Tumor size may not be strongly associated with hypoxic state. HIF-1α (dpeaa)DE-He213 DNA binding HIF-1 activity (dpeaa)DE-He213 Ovary (dpeaa)DE-He213 Yasuda, Masanori verfasserin aut Fujita, Mariko verfasserin aut Hirasawa, Takeshi verfasserin aut Kajiwara, Hiroshi verfasserin aut Hirabayashi, Kenichi verfasserin aut Ogane, Naoki verfasserin aut Shimizu, Michio verfasserin aut Asanuma, Hideki verfasserin aut Murakami, Masaru verfasserin aut Takekoshi, Susumu verfasserin aut Mikami, Mikio verfasserin aut Osamura, R. Yoshiyuki verfasserin aut Enthalten in Archives of gynecology and obstetrics Berlin : Springer, 1870 279(2008), 6 vom: 21. Okt., Seite 789-796 (DE-627)253390060 (DE-600)1458450-5 1432-0711 nnns volume:279 year:2008 number:6 day:21 month:10 pages:789-796 https://dx.doi.org/10.1007/s00404-008-0816-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_711 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.92 ASE AR 279 2008 6 21 10 789-796 |
| spelling |
10.1007/s00404-008-0816-z doi (DE-627)SPR005098963 (SPR)s00404-008-0816-z-e DE-627 ger DE-627 rakwb eng 610 ASE 44.92 bkl Miyazawa, Masaki verfasserin aut Association of hypoxia-inducible factor-1 expression with histology in epithelial ovarian tumors: a quantitative analysis of HIF-1 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background Hypoxia-inducible factor-1 (HIF-1) is an essential transcription factor that mediates cellular and systemic homeostatic responses to reduced oxygen availability in mammals. So far, using immunohistochemistry we have analyzed the association of HIF-1α expression with histological type among epithelial ovarian tumors. In the present study, quantitative analyses of activated HIF-1 level in the nucleus and of accumulated HIF-1α level in the cytoplasm were performed to clarify whether or not the hypoxic state would be correlated to histology, malignancy, and tumor size in epithelial ovarian tumors. Method HIF-1 level in the nucleus was analyzed using DNA binding assay, and HIF-1α level in the cytoplasm was measured by ELISA for a total of 36 epithelial ovarian tumors as follows: 5 serous adenocarcinomas (SEAs), 7 clear cell adenocarcinomas (CLAs), 7 endometrioid adenocarcinomas (ENAs), 4 mucinous adenocarcinomas (MUAs), 2 mucinous borderline tumors (MBTs), and 11 mucinous adenomas. Results HIF-1 level (mg/ml) in the nucleus and HIF-1α level (mg/ml) in the cytoplasm were on average 0.116 and 0.178 for SEAs, 0.328 and 0.306 for CLAs, 0.171 and 0.305 for ENAs, 0.097 and 0.176 for MUAs, 0.224 and 0.180 for mucinous borderline tumors, 0.152 and 0.154 for mucinous adenomas. CLAs showed the highest levels for both of HIF-1 and HIF-1α, while MUAs showed the lowest levels of both. Mucinous adenomas were higher in HIF-1 than MUAs. Conclusion Hypoxic state was considered to be closely related to histological type of epithelial ovarian tumors, suggesting that CLAs may be most hypoxic. In the comparison of mucinous tumors, malignancies would not always become most hypoxic. Tumor size may not be strongly associated with hypoxic state. HIF-1α (dpeaa)DE-He213 DNA binding HIF-1 activity (dpeaa)DE-He213 Ovary (dpeaa)DE-He213 Yasuda, Masanori verfasserin aut Fujita, Mariko verfasserin aut Hirasawa, Takeshi verfasserin aut Kajiwara, Hiroshi verfasserin aut Hirabayashi, Kenichi verfasserin aut Ogane, Naoki verfasserin aut Shimizu, Michio verfasserin aut Asanuma, Hideki verfasserin aut Murakami, Masaru verfasserin aut Takekoshi, Susumu verfasserin aut Mikami, Mikio verfasserin aut Osamura, R. Yoshiyuki verfasserin aut Enthalten in Archives of gynecology and obstetrics Berlin : Springer, 1870 279(2008), 6 vom: 21. Okt., Seite 789-796 (DE-627)253390060 (DE-600)1458450-5 1432-0711 nnns volume:279 year:2008 number:6 day:21 month:10 pages:789-796 https://dx.doi.org/10.1007/s00404-008-0816-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_711 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.92 ASE AR 279 2008 6 21 10 789-796 |
| allfields_unstemmed |
10.1007/s00404-008-0816-z doi (DE-627)SPR005098963 (SPR)s00404-008-0816-z-e DE-627 ger DE-627 rakwb eng 610 ASE 44.92 bkl Miyazawa, Masaki verfasserin aut Association of hypoxia-inducible factor-1 expression with histology in epithelial ovarian tumors: a quantitative analysis of HIF-1 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background Hypoxia-inducible factor-1 (HIF-1) is an essential transcription factor that mediates cellular and systemic homeostatic responses to reduced oxygen availability in mammals. So far, using immunohistochemistry we have analyzed the association of HIF-1α expression with histological type among epithelial ovarian tumors. In the present study, quantitative analyses of activated HIF-1 level in the nucleus and of accumulated HIF-1α level in the cytoplasm were performed to clarify whether or not the hypoxic state would be correlated to histology, malignancy, and tumor size in epithelial ovarian tumors. Method HIF-1 level in the nucleus was analyzed using DNA binding assay, and HIF-1α level in the cytoplasm was measured by ELISA for a total of 36 epithelial ovarian tumors as follows: 5 serous adenocarcinomas (SEAs), 7 clear cell adenocarcinomas (CLAs), 7 endometrioid adenocarcinomas (ENAs), 4 mucinous adenocarcinomas (MUAs), 2 mucinous borderline tumors (MBTs), and 11 mucinous adenomas. Results HIF-1 level (mg/ml) in the nucleus and HIF-1α level (mg/ml) in the cytoplasm were on average 0.116 and 0.178 for SEAs, 0.328 and 0.306 for CLAs, 0.171 and 0.305 for ENAs, 0.097 and 0.176 for MUAs, 0.224 and 0.180 for mucinous borderline tumors, 0.152 and 0.154 for mucinous adenomas. CLAs showed the highest levels for both of HIF-1 and HIF-1α, while MUAs showed the lowest levels of both. Mucinous adenomas were higher in HIF-1 than MUAs. Conclusion Hypoxic state was considered to be closely related to histological type of epithelial ovarian tumors, suggesting that CLAs may be most hypoxic. In the comparison of mucinous tumors, malignancies would not always become most hypoxic. Tumor size may not be strongly associated with hypoxic state. HIF-1α (dpeaa)DE-He213 DNA binding HIF-1 activity (dpeaa)DE-He213 Ovary (dpeaa)DE-He213 Yasuda, Masanori verfasserin aut Fujita, Mariko verfasserin aut Hirasawa, Takeshi verfasserin aut Kajiwara, Hiroshi verfasserin aut Hirabayashi, Kenichi verfasserin aut Ogane, Naoki verfasserin aut Shimizu, Michio verfasserin aut Asanuma, Hideki verfasserin aut Murakami, Masaru verfasserin aut Takekoshi, Susumu verfasserin aut Mikami, Mikio verfasserin aut Osamura, R. Yoshiyuki verfasserin aut Enthalten in Archives of gynecology and obstetrics Berlin : Springer, 1870 279(2008), 6 vom: 21. Okt., Seite 789-796 (DE-627)253390060 (DE-600)1458450-5 1432-0711 nnns volume:279 year:2008 number:6 day:21 month:10 pages:789-796 https://dx.doi.org/10.1007/s00404-008-0816-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_711 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.92 ASE AR 279 2008 6 21 10 789-796 |
| allfieldsGer |
10.1007/s00404-008-0816-z doi (DE-627)SPR005098963 (SPR)s00404-008-0816-z-e DE-627 ger DE-627 rakwb eng 610 ASE 44.92 bkl Miyazawa, Masaki verfasserin aut Association of hypoxia-inducible factor-1 expression with histology in epithelial ovarian tumors: a quantitative analysis of HIF-1 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background Hypoxia-inducible factor-1 (HIF-1) is an essential transcription factor that mediates cellular and systemic homeostatic responses to reduced oxygen availability in mammals. So far, using immunohistochemistry we have analyzed the association of HIF-1α expression with histological type among epithelial ovarian tumors. In the present study, quantitative analyses of activated HIF-1 level in the nucleus and of accumulated HIF-1α level in the cytoplasm were performed to clarify whether or not the hypoxic state would be correlated to histology, malignancy, and tumor size in epithelial ovarian tumors. Method HIF-1 level in the nucleus was analyzed using DNA binding assay, and HIF-1α level in the cytoplasm was measured by ELISA for a total of 36 epithelial ovarian tumors as follows: 5 serous adenocarcinomas (SEAs), 7 clear cell adenocarcinomas (CLAs), 7 endometrioid adenocarcinomas (ENAs), 4 mucinous adenocarcinomas (MUAs), 2 mucinous borderline tumors (MBTs), and 11 mucinous adenomas. Results HIF-1 level (mg/ml) in the nucleus and HIF-1α level (mg/ml) in the cytoplasm were on average 0.116 and 0.178 for SEAs, 0.328 and 0.306 for CLAs, 0.171 and 0.305 for ENAs, 0.097 and 0.176 for MUAs, 0.224 and 0.180 for mucinous borderline tumors, 0.152 and 0.154 for mucinous adenomas. CLAs showed the highest levels for both of HIF-1 and HIF-1α, while MUAs showed the lowest levels of both. Mucinous adenomas were higher in HIF-1 than MUAs. Conclusion Hypoxic state was considered to be closely related to histological type of epithelial ovarian tumors, suggesting that CLAs may be most hypoxic. In the comparison of mucinous tumors, malignancies would not always become most hypoxic. Tumor size may not be strongly associated with hypoxic state. HIF-1α (dpeaa)DE-He213 DNA binding HIF-1 activity (dpeaa)DE-He213 Ovary (dpeaa)DE-He213 Yasuda, Masanori verfasserin aut Fujita, Mariko verfasserin aut Hirasawa, Takeshi verfasserin aut Kajiwara, Hiroshi verfasserin aut Hirabayashi, Kenichi verfasserin aut Ogane, Naoki verfasserin aut Shimizu, Michio verfasserin aut Asanuma, Hideki verfasserin aut Murakami, Masaru verfasserin aut Takekoshi, Susumu verfasserin aut Mikami, Mikio verfasserin aut Osamura, R. Yoshiyuki verfasserin aut Enthalten in Archives of gynecology and obstetrics Berlin : Springer, 1870 279(2008), 6 vom: 21. Okt., Seite 789-796 (DE-627)253390060 (DE-600)1458450-5 1432-0711 nnns volume:279 year:2008 number:6 day:21 month:10 pages:789-796 https://dx.doi.org/10.1007/s00404-008-0816-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_711 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.92 ASE AR 279 2008 6 21 10 789-796 |
| allfieldsSound |
10.1007/s00404-008-0816-z doi (DE-627)SPR005098963 (SPR)s00404-008-0816-z-e DE-627 ger DE-627 rakwb eng 610 ASE 44.92 bkl Miyazawa, Masaki verfasserin aut Association of hypoxia-inducible factor-1 expression with histology in epithelial ovarian tumors: a quantitative analysis of HIF-1 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background Hypoxia-inducible factor-1 (HIF-1) is an essential transcription factor that mediates cellular and systemic homeostatic responses to reduced oxygen availability in mammals. So far, using immunohistochemistry we have analyzed the association of HIF-1α expression with histological type among epithelial ovarian tumors. In the present study, quantitative analyses of activated HIF-1 level in the nucleus and of accumulated HIF-1α level in the cytoplasm were performed to clarify whether or not the hypoxic state would be correlated to histology, malignancy, and tumor size in epithelial ovarian tumors. Method HIF-1 level in the nucleus was analyzed using DNA binding assay, and HIF-1α level in the cytoplasm was measured by ELISA for a total of 36 epithelial ovarian tumors as follows: 5 serous adenocarcinomas (SEAs), 7 clear cell adenocarcinomas (CLAs), 7 endometrioid adenocarcinomas (ENAs), 4 mucinous adenocarcinomas (MUAs), 2 mucinous borderline tumors (MBTs), and 11 mucinous adenomas. Results HIF-1 level (mg/ml) in the nucleus and HIF-1α level (mg/ml) in the cytoplasm were on average 0.116 and 0.178 for SEAs, 0.328 and 0.306 for CLAs, 0.171 and 0.305 for ENAs, 0.097 and 0.176 for MUAs, 0.224 and 0.180 for mucinous borderline tumors, 0.152 and 0.154 for mucinous adenomas. CLAs showed the highest levels for both of HIF-1 and HIF-1α, while MUAs showed the lowest levels of both. Mucinous adenomas were higher in HIF-1 than MUAs. Conclusion Hypoxic state was considered to be closely related to histological type of epithelial ovarian tumors, suggesting that CLAs may be most hypoxic. In the comparison of mucinous tumors, malignancies would not always become most hypoxic. Tumor size may not be strongly associated with hypoxic state. HIF-1α (dpeaa)DE-He213 DNA binding HIF-1 activity (dpeaa)DE-He213 Ovary (dpeaa)DE-He213 Yasuda, Masanori verfasserin aut Fujita, Mariko verfasserin aut Hirasawa, Takeshi verfasserin aut Kajiwara, Hiroshi verfasserin aut Hirabayashi, Kenichi verfasserin aut Ogane, Naoki verfasserin aut Shimizu, Michio verfasserin aut Asanuma, Hideki verfasserin aut Murakami, Masaru verfasserin aut Takekoshi, Susumu verfasserin aut Mikami, Mikio verfasserin aut Osamura, R. Yoshiyuki verfasserin aut Enthalten in Archives of gynecology and obstetrics Berlin : Springer, 1870 279(2008), 6 vom: 21. Okt., Seite 789-796 (DE-627)253390060 (DE-600)1458450-5 1432-0711 nnns volume:279 year:2008 number:6 day:21 month:10 pages:789-796 https://dx.doi.org/10.1007/s00404-008-0816-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_711 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.92 ASE AR 279 2008 6 21 10 789-796 |
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Enthalten in Archives of gynecology and obstetrics 279(2008), 6 vom: 21. Okt., Seite 789-796 volume:279 year:2008 number:6 day:21 month:10 pages:789-796 |
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Enthalten in Archives of gynecology and obstetrics 279(2008), 6 vom: 21. Okt., Seite 789-796 volume:279 year:2008 number:6 day:21 month:10 pages:789-796 |
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HIF-1α DNA binding HIF-1 activity Ovary |
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Archives of gynecology and obstetrics |
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Miyazawa, Masaki @@aut@@ Yasuda, Masanori @@aut@@ Fujita, Mariko @@aut@@ Hirasawa, Takeshi @@aut@@ Kajiwara, Hiroshi @@aut@@ Hirabayashi, Kenichi @@aut@@ Ogane, Naoki @@aut@@ Shimizu, Michio @@aut@@ Asanuma, Hideki @@aut@@ Murakami, Masaru @@aut@@ Takekoshi, Susumu @@aut@@ Mikami, Mikio @@aut@@ Osamura, R. Yoshiyuki @@aut@@ |
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2008-10-21T00:00:00Z |
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So far, using immunohistochemistry we have analyzed the association of HIF-1α expression with histological type among epithelial ovarian tumors. In the present study, quantitative analyses of activated HIF-1 level in the nucleus and of accumulated HIF-1α level in the cytoplasm were performed to clarify whether or not the hypoxic state would be correlated to histology, malignancy, and tumor size in epithelial ovarian tumors. Method HIF-1 level in the nucleus was analyzed using DNA binding assay, and HIF-1α level in the cytoplasm was measured by ELISA for a total of 36 epithelial ovarian tumors as follows: 5 serous adenocarcinomas (SEAs), 7 clear cell adenocarcinomas (CLAs), 7 endometrioid adenocarcinomas (ENAs), 4 mucinous adenocarcinomas (MUAs), 2 mucinous borderline tumors (MBTs), and 11 mucinous adenomas. Results HIF-1 level (mg/ml) in the nucleus and HIF-1α level (mg/ml) in the cytoplasm were on average 0.116 and 0.178 for SEAs, 0.328 and 0.306 for CLAs, 0.171 and 0.305 for ENAs, 0.097 and 0.176 for MUAs, 0.224 and 0.180 for mucinous borderline tumors, 0.152 and 0.154 for mucinous adenomas. CLAs showed the highest levels for both of HIF-1 and HIF-1α, while MUAs showed the lowest levels of both. Mucinous adenomas were higher in HIF-1 than MUAs. Conclusion Hypoxic state was considered to be closely related to histological type of epithelial ovarian tumors, suggesting that CLAs may be most hypoxic. In the comparison of mucinous tumors, malignancies would not always become most hypoxic. 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|
| author |
Miyazawa, Masaki |
| spellingShingle |
Miyazawa, Masaki ddc 610 bkl 44.92 misc HIF-1α misc DNA binding HIF-1 activity misc Ovary Association of hypoxia-inducible factor-1 expression with histology in epithelial ovarian tumors: a quantitative analysis of HIF-1 |
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1432-0711 |
| topic_title |
610 ASE 44.92 bkl Association of hypoxia-inducible factor-1 expression with histology in epithelial ovarian tumors: a quantitative analysis of HIF-1 HIF-1α (dpeaa)DE-He213 DNA binding HIF-1 activity (dpeaa)DE-He213 Ovary (dpeaa)DE-He213 |
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ddc 610 bkl 44.92 misc HIF-1α misc DNA binding HIF-1 activity misc Ovary |
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ddc 610 bkl 44.92 misc HIF-1α misc DNA binding HIF-1 activity misc Ovary |
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ddc 610 bkl 44.92 misc HIF-1α misc DNA binding HIF-1 activity misc Ovary |
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Archives of gynecology and obstetrics |
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Archives of gynecology and obstetrics |
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Association of hypoxia-inducible factor-1 expression with histology in epithelial ovarian tumors: a quantitative analysis of HIF-1 |
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(DE-627)SPR005098963 (SPR)s00404-008-0816-z-e |
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Association of hypoxia-inducible factor-1 expression with histology in epithelial ovarian tumors: a quantitative analysis of HIF-1 |
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Miyazawa, Masaki |
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Archives of gynecology and obstetrics |
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Archives of gynecology and obstetrics |
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eng |
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600 - Technology |
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2008 |
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Miyazawa, Masaki Yasuda, Masanori Fujita, Mariko Hirasawa, Takeshi Kajiwara, Hiroshi Hirabayashi, Kenichi Ogane, Naoki Shimizu, Michio Asanuma, Hideki Murakami, Masaru Takekoshi, Susumu Mikami, Mikio Osamura, R. Yoshiyuki |
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Miyazawa, Masaki |
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10.1007/s00404-008-0816-z |
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610 |
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verfasserin |
| title_sort |
association of hypoxia-inducible factor-1 expression with histology in epithelial ovarian tumors: a quantitative analysis of hif-1 |
| title_auth |
Association of hypoxia-inducible factor-1 expression with histology in epithelial ovarian tumors: a quantitative analysis of HIF-1 |
| abstract |
Background Hypoxia-inducible factor-1 (HIF-1) is an essential transcription factor that mediates cellular and systemic homeostatic responses to reduced oxygen availability in mammals. So far, using immunohistochemistry we have analyzed the association of HIF-1α expression with histological type among epithelial ovarian tumors. In the present study, quantitative analyses of activated HIF-1 level in the nucleus and of accumulated HIF-1α level in the cytoplasm were performed to clarify whether or not the hypoxic state would be correlated to histology, malignancy, and tumor size in epithelial ovarian tumors. Method HIF-1 level in the nucleus was analyzed using DNA binding assay, and HIF-1α level in the cytoplasm was measured by ELISA for a total of 36 epithelial ovarian tumors as follows: 5 serous adenocarcinomas (SEAs), 7 clear cell adenocarcinomas (CLAs), 7 endometrioid adenocarcinomas (ENAs), 4 mucinous adenocarcinomas (MUAs), 2 mucinous borderline tumors (MBTs), and 11 mucinous adenomas. Results HIF-1 level (mg/ml) in the nucleus and HIF-1α level (mg/ml) in the cytoplasm were on average 0.116 and 0.178 for SEAs, 0.328 and 0.306 for CLAs, 0.171 and 0.305 for ENAs, 0.097 and 0.176 for MUAs, 0.224 and 0.180 for mucinous borderline tumors, 0.152 and 0.154 for mucinous adenomas. CLAs showed the highest levels for both of HIF-1 and HIF-1α, while MUAs showed the lowest levels of both. Mucinous adenomas were higher in HIF-1 than MUAs. Conclusion Hypoxic state was considered to be closely related to histological type of epithelial ovarian tumors, suggesting that CLAs may be most hypoxic. In the comparison of mucinous tumors, malignancies would not always become most hypoxic. Tumor size may not be strongly associated with hypoxic state. |
| abstractGer |
Background Hypoxia-inducible factor-1 (HIF-1) is an essential transcription factor that mediates cellular and systemic homeostatic responses to reduced oxygen availability in mammals. So far, using immunohistochemistry we have analyzed the association of HIF-1α expression with histological type among epithelial ovarian tumors. In the present study, quantitative analyses of activated HIF-1 level in the nucleus and of accumulated HIF-1α level in the cytoplasm were performed to clarify whether or not the hypoxic state would be correlated to histology, malignancy, and tumor size in epithelial ovarian tumors. Method HIF-1 level in the nucleus was analyzed using DNA binding assay, and HIF-1α level in the cytoplasm was measured by ELISA for a total of 36 epithelial ovarian tumors as follows: 5 serous adenocarcinomas (SEAs), 7 clear cell adenocarcinomas (CLAs), 7 endometrioid adenocarcinomas (ENAs), 4 mucinous adenocarcinomas (MUAs), 2 mucinous borderline tumors (MBTs), and 11 mucinous adenomas. Results HIF-1 level (mg/ml) in the nucleus and HIF-1α level (mg/ml) in the cytoplasm were on average 0.116 and 0.178 for SEAs, 0.328 and 0.306 for CLAs, 0.171 and 0.305 for ENAs, 0.097 and 0.176 for MUAs, 0.224 and 0.180 for mucinous borderline tumors, 0.152 and 0.154 for mucinous adenomas. CLAs showed the highest levels for both of HIF-1 and HIF-1α, while MUAs showed the lowest levels of both. Mucinous adenomas were higher in HIF-1 than MUAs. Conclusion Hypoxic state was considered to be closely related to histological type of epithelial ovarian tumors, suggesting that CLAs may be most hypoxic. In the comparison of mucinous tumors, malignancies would not always become most hypoxic. Tumor size may not be strongly associated with hypoxic state. |
| abstract_unstemmed |
Background Hypoxia-inducible factor-1 (HIF-1) is an essential transcription factor that mediates cellular and systemic homeostatic responses to reduced oxygen availability in mammals. So far, using immunohistochemistry we have analyzed the association of HIF-1α expression with histological type among epithelial ovarian tumors. In the present study, quantitative analyses of activated HIF-1 level in the nucleus and of accumulated HIF-1α level in the cytoplasm were performed to clarify whether or not the hypoxic state would be correlated to histology, malignancy, and tumor size in epithelial ovarian tumors. Method HIF-1 level in the nucleus was analyzed using DNA binding assay, and HIF-1α level in the cytoplasm was measured by ELISA for a total of 36 epithelial ovarian tumors as follows: 5 serous adenocarcinomas (SEAs), 7 clear cell adenocarcinomas (CLAs), 7 endometrioid adenocarcinomas (ENAs), 4 mucinous adenocarcinomas (MUAs), 2 mucinous borderline tumors (MBTs), and 11 mucinous adenomas. Results HIF-1 level (mg/ml) in the nucleus and HIF-1α level (mg/ml) in the cytoplasm were on average 0.116 and 0.178 for SEAs, 0.328 and 0.306 for CLAs, 0.171 and 0.305 for ENAs, 0.097 and 0.176 for MUAs, 0.224 and 0.180 for mucinous borderline tumors, 0.152 and 0.154 for mucinous adenomas. CLAs showed the highest levels for both of HIF-1 and HIF-1α, while MUAs showed the lowest levels of both. Mucinous adenomas were higher in HIF-1 than MUAs. Conclusion Hypoxic state was considered to be closely related to histological type of epithelial ovarian tumors, suggesting that CLAs may be most hypoxic. In the comparison of mucinous tumors, malignancies would not always become most hypoxic. Tumor size may not be strongly associated with hypoxic state. |
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Association of hypoxia-inducible factor-1 expression with histology in epithelial ovarian tumors: a quantitative analysis of HIF-1 |
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https://dx.doi.org/10.1007/s00404-008-0816-z |
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Yasuda, Masanori Fujita, Mariko Hirasawa, Takeshi Kajiwara, Hiroshi Hirabayashi, Kenichi Ogane, Naoki Shimizu, Michio Asanuma, Hideki Murakami, Masaru Takekoshi, Susumu Mikami, Mikio Osamura, R. Yoshiyuki |
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Yasuda, Masanori Fujita, Mariko Hirasawa, Takeshi Kajiwara, Hiroshi Hirabayashi, Kenichi Ogane, Naoki Shimizu, Michio Asanuma, Hideki Murakami, Masaru Takekoshi, Susumu Mikami, Mikio Osamura, R. Yoshiyuki |
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10.1007/s00404-008-0816-z |
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2024-07-03T13:59:16.834Z |
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So far, using immunohistochemistry we have analyzed the association of HIF-1α expression with histological type among epithelial ovarian tumors. In the present study, quantitative analyses of activated HIF-1 level in the nucleus and of accumulated HIF-1α level in the cytoplasm were performed to clarify whether or not the hypoxic state would be correlated to histology, malignancy, and tumor size in epithelial ovarian tumors. Method HIF-1 level in the nucleus was analyzed using DNA binding assay, and HIF-1α level in the cytoplasm was measured by ELISA for a total of 36 epithelial ovarian tumors as follows: 5 serous adenocarcinomas (SEAs), 7 clear cell adenocarcinomas (CLAs), 7 endometrioid adenocarcinomas (ENAs), 4 mucinous adenocarcinomas (MUAs), 2 mucinous borderline tumors (MBTs), and 11 mucinous adenomas. 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| score |
7.400278 |