Immunohistochemical detection of intrathrombotic IL-6 and its application to thrombus age estimation

Abstract We immunohistochemically examined intrathrombotic IL-6 expression using a murine model of deep vein thrombosis induced by the ligation of the inferior vena cava (IVC) and discussed the availability of intrathrombotic IL-6 for thrombus age estimation. IL-$ 6^{+} $ cells could be first detect...
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Autor*in:	Nosaka, Mizuho [verfasserIn] Ishida, Yuko [verfasserIn] Kimura, Akihiko [verfasserIn] Hama, Mizuki [verfasserIn] Kawaguchi, Takashi [verfasserIn] Yamamoto, Hiroki [verfasserIn] Kuninaka, Yumi [verfasserIn] Shimada, Emi [verfasserIn] Kondo, Toshikazu [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2015

Schlagwörter:	Forensic pathology Thrombus age estimation Immunohistochemistry IL-6 Macrophages

Übergeordnetes Werk:	Enthalten in: International journal of legal medicine - Berlin : Springer, 1922, 129(2015), 5 vom: 24. Jan., Seite 1021-1025
Übergeordnetes Werk:	volume:129 ; year:2015 ; number:5 ; day:24 ; month:01 ; pages:1021-1025

Links:	Volltext

DOI / URN:	10.1007/s00414-015-1147-9

Katalog-ID:	SPR005300487

Internformat


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245	1	0	\|a Immunohistochemical detection of intrathrombotic IL-6 and its application to thrombus age estimation
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520			\|a Abstract We immunohistochemically examined intrathrombotic IL-6 expression using a murine model of deep vein thrombosis induced by the ligation of the inferior vena cava (IVC) and discussed the availability of intrathrombotic IL-6 for thrombus age estimation. IL-$ 6^{+} $ cells could be first detected at 1 day after IVC ligation in three of five samples, and all samples with postligation intervals of 3 days or more had intrathrombotic IL-$ 6^{+} $ cells. Thereafter, the numbers of IL-$ 6^{+} $ cells were elevated with the increase of postligation intervals. Double-color immunofluorescence analyses demonstrated that IL-6 was mainly expressed by intrathrombotic macrophages. In all samples with postligation intervals of 5 days or less, the IL-6/macrophage ratio (IL-6/Mϕ) was <0.5. In contrast, the IL-6/Mϕ was greater than 0.5 at ≥7 days after the IVC ligation. These observations implied that IL-6/Mϕ ratios of >0.5 would strongly indicate thrombus ages of ≥7 days. Reciprocally, the ratios of less than 0.5 would suggest thrombus ages of ≤5 days. The present study demonstrated that the immunohistochemical detection of IL-6 was suitable to estimate the age of venous thrombi.
650		4	\|a Forensic pathology \|7 (dpeaa)DE-He213
650		4	\|a Thrombus age estimation \|7 (dpeaa)DE-He213
650		4	\|a Immunohistochemistry \|7 (dpeaa)DE-He213
650		4	\|a IL-6 \|7 (dpeaa)DE-He213
650		4	\|a Macrophages \|7 (dpeaa)DE-He213
700	1		\|a Ishida, Yuko \|e verfasserin \|4 aut
700	1		\|a Kimura, Akihiko \|e verfasserin \|4 aut
700	1		\|a Hama, Mizuki \|e verfasserin \|4 aut
700	1		\|a Kawaguchi, Takashi \|e verfasserin \|4 aut
700	1		\|a Yamamoto, Hiroki \|e verfasserin \|4 aut
700	1		\|a Kuninaka, Yumi \|e verfasserin \|4 aut
700	1		\|a Shimada, Emi \|e verfasserin \|4 aut
700	1		\|a Kondo, Toshikazu \|e verfasserin \|4 aut
773	0	8	\|i Enthalten in \|t International journal of legal medicine \|d Berlin : Springer, 1922 \|g 129(2015), 5 vom: 24. Jan., Seite 1021-1025 \|w (DE-627)253724295 \|w (DE-600)1459222-8 \|x 1437-1596 \|7 nnns
773	1	8	\|g volume:129 \|g year:2015 \|g number:5 \|g day:24 \|g month:01 \|g pages:1021-1025
856	4	0	\|u https://dx.doi.org/10.1007/s00414-015-1147-9 \|z lizenzpflichtig \|3 Volltext
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912			\|a GBV_ILN_23
912			\|a GBV_ILN_24
912			\|a GBV_ILN_31
912			\|a GBV_ILN_32
912			\|a GBV_ILN_39
912			\|a GBV_ILN_40
912			\|a GBV_ILN_60
912			\|a GBV_ILN_62
912			\|a GBV_ILN_63
912			\|a GBV_ILN_65
912			\|a GBV_ILN_69
912			\|a GBV_ILN_70
912			\|a GBV_ILN_73
912			\|a GBV_ILN_74
912			\|a GBV_ILN_90
912			\|a GBV_ILN_95
912			\|a GBV_ILN_100
912			\|a GBV_ILN_101
912			\|a GBV_ILN_105
912			\|a GBV_ILN_110
912			\|a GBV_ILN_120
912			\|a GBV_ILN_138
912			\|a GBV_ILN_150
912			\|a GBV_ILN_151
912			\|a GBV_ILN_152
912			\|a GBV_ILN_161
912			\|a GBV_ILN_170
912			\|a GBV_ILN_171
912			\|a GBV_ILN_187
912			\|a GBV_ILN_213
912			\|a GBV_ILN_224
912			\|a GBV_ILN_230
912			\|a GBV_ILN_250
912			\|a GBV_ILN_267
912			\|a GBV_ILN_281
912			\|a GBV_ILN_285
912			\|a GBV_ILN_293
912			\|a GBV_ILN_370
912			\|a GBV_ILN_602
912			\|a GBV_ILN_636
912			\|a GBV_ILN_702
912			\|a GBV_ILN_711
912			\|a GBV_ILN_2001
912			\|a GBV_ILN_2003
912			\|a GBV_ILN_2004
912			\|a GBV_ILN_2005
912			\|a GBV_ILN_2006
912			\|a GBV_ILN_2007
912			\|a GBV_ILN_2008
912			\|a GBV_ILN_2009
912			\|a GBV_ILN_2010
912			\|a GBV_ILN_2011
912			\|a GBV_ILN_2014
912			\|a GBV_ILN_2015
912			\|a GBV_ILN_2020
912			\|a GBV_ILN_2021
912			\|a GBV_ILN_2025
912			\|a GBV_ILN_2026
912			\|a GBV_ILN_2027
912			\|a GBV_ILN_2031
912			\|a GBV_ILN_2034
912			\|a GBV_ILN_2037
912			\|a GBV_ILN_2038
912			\|a GBV_ILN_2039
912			\|a GBV_ILN_2044
912			\|a GBV_ILN_2048
912			\|a GBV_ILN_2049
912			\|a GBV_ILN_2050
912			\|a GBV_ILN_2055
912			\|a GBV_ILN_2057
912			\|a GBV_ILN_2059
912			\|a GBV_ILN_2061
912			\|a GBV_ILN_2064
912			\|a GBV_ILN_2065
912			\|a GBV_ILN_2068
912			\|a GBV_ILN_2070
912			\|a GBV_ILN_2086
912			\|a GBV_ILN_2088
912			\|a GBV_ILN_2093
912			\|a GBV_ILN_2106
912			\|a GBV_ILN_2107
912			\|a GBV_ILN_2108
912			\|a GBV_ILN_2110
912			\|a GBV_ILN_2111
912			\|a GBV_ILN_2112
912			\|a GBV_ILN_2113
912			\|a GBV_ILN_2116
912			\|a GBV_ILN_2118
912			\|a GBV_ILN_2119
912			\|a GBV_ILN_2122
912			\|a GBV_ILN_2129
912			\|a GBV_ILN_2143
912			\|a GBV_ILN_2144
912			\|a GBV_ILN_2147
912			\|a GBV_ILN_2148
912			\|a GBV_ILN_2152
912			\|a GBV_ILN_2153
912			\|a GBV_ILN_2188
912			\|a GBV_ILN_2190
912			\|a GBV_ILN_2232
912			\|a GBV_ILN_2336
912			\|a GBV_ILN_2446
912			\|a GBV_ILN_2470
912			\|a GBV_ILN_2472
912			\|a GBV_ILN_2507
912			\|a GBV_ILN_2522
912			\|a GBV_ILN_2548
912			\|a GBV_ILN_4012
912			\|a GBV_ILN_4035
912			\|a GBV_ILN_4037
912			\|a GBV_ILN_4046
912			\|a GBV_ILN_4112
912			\|a GBV_ILN_4125
912			\|a GBV_ILN_4126
912			\|a GBV_ILN_4242
912			\|a GBV_ILN_4246
912			\|a GBV_ILN_4249
912			\|a GBV_ILN_4251
912			\|a GBV_ILN_4305
912			\|a GBV_ILN_4306
912			\|a GBV_ILN_4307
912			\|a GBV_ILN_4313
912			\|a GBV_ILN_4322
912			\|a GBV_ILN_4323
912			\|a GBV_ILN_4324
912			\|a GBV_ILN_4325
912			\|a GBV_ILN_4326
912			\|a GBV_ILN_4328
912			\|a GBV_ILN_4333
912			\|a GBV_ILN_4334
912			\|a GBV_ILN_4335
912			\|a GBV_ILN_4336
912			\|a GBV_ILN_4338
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936	b	k	\|a 44.00 \|q ASE
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936	b	k	\|a 44.72 \|q ASE
951			\|a AR
952			\|d 129 \|j 2015 \|e 5 \|b 24 \|c 01 \|h 1021-1025

Indexfelder

author_variant	m n mn y i yi a k ak m h mh t k tk h y hy y k yk e s es t k tk
matchkey_str	article:14371596:2015----::muoitceiadtcinfnrtrmoii6nisplctot
hierarchy_sort_str	2015
bklnumber	44.00 86.00 44.72
publishDate	2015
allfields	10.1007/s00414-015-1147-9 doi (DE-627)SPR005300487 (SPR)s00414-015-1147-9-e DE-627 ger DE-627 rakwb eng 340 610 ASE 44.00 bkl 86.00 bkl 44.72 bkl Nosaka, Mizuho verfasserin aut Immunohistochemical detection of intrathrombotic IL-6 and its application to thrombus age estimation 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract We immunohistochemically examined intrathrombotic IL-6 expression using a murine model of deep vein thrombosis induced by the ligation of the inferior vena cava (IVC) and discussed the availability of intrathrombotic IL-6 for thrombus age estimation. IL-$ 6^{+} $ cells could be first detected at 1 day after IVC ligation in three of five samples, and all samples with postligation intervals of 3 days or more had intrathrombotic IL-$ 6^{+} $ cells. Thereafter, the numbers of IL-$ 6^{+} $ cells were elevated with the increase of postligation intervals. Double-color immunofluorescence analyses demonstrated that IL-6 was mainly expressed by intrathrombotic macrophages. In all samples with postligation intervals of 5 days or less, the IL-6/macrophage ratio (IL-6/Mϕ) was <0.5. In contrast, the IL-6/Mϕ was greater than 0.5 at ≥7 days after the IVC ligation. These observations implied that IL-6/Mϕ ratios of >0.5 would strongly indicate thrombus ages of ≥7 days. Reciprocally, the ratios of less than 0.5 would suggest thrombus ages of ≤5 days. The present study demonstrated that the immunohistochemical detection of IL-6 was suitable to estimate the age of venous thrombi. Forensic pathology (dpeaa)DE-He213 Thrombus age estimation (dpeaa)DE-He213 Immunohistochemistry (dpeaa)DE-He213 IL-6 (dpeaa)DE-He213 Macrophages (dpeaa)DE-He213 Ishida, Yuko verfasserin aut Kimura, Akihiko verfasserin aut Hama, Mizuki verfasserin aut Kawaguchi, Takashi verfasserin aut Yamamoto, Hiroki verfasserin aut Kuninaka, Yumi verfasserin aut Shimada, Emi verfasserin aut Kondo, Toshikazu verfasserin aut Enthalten in International journal of legal medicine Berlin : Springer, 1922 129(2015), 5 vom: 24. Jan., Seite 1021-1025 (DE-627)253724295 (DE-600)1459222-8 1437-1596 nnns volume:129 year:2015 number:5 day:24 month:01 pages:1021-1025 https://dx.doi.org/10.1007/s00414-015-1147-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_711 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.00 ASE 86.00 ASE 44.72 ASE AR 129 2015 5 24 01 1021-1025
spelling	10.1007/s00414-015-1147-9 doi (DE-627)SPR005300487 (SPR)s00414-015-1147-9-e DE-627 ger DE-627 rakwb eng 340 610 ASE 44.00 bkl 86.00 bkl 44.72 bkl Nosaka, Mizuho verfasserin aut Immunohistochemical detection of intrathrombotic IL-6 and its application to thrombus age estimation 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract We immunohistochemically examined intrathrombotic IL-6 expression using a murine model of deep vein thrombosis induced by the ligation of the inferior vena cava (IVC) and discussed the availability of intrathrombotic IL-6 for thrombus age estimation. IL-$ 6^{+} $ cells could be first detected at 1 day after IVC ligation in three of five samples, and all samples with postligation intervals of 3 days or more had intrathrombotic IL-$ 6^{+} $ cells. Thereafter, the numbers of IL-$ 6^{+} $ cells were elevated with the increase of postligation intervals. Double-color immunofluorescence analyses demonstrated that IL-6 was mainly expressed by intrathrombotic macrophages. In all samples with postligation intervals of 5 days or less, the IL-6/macrophage ratio (IL-6/Mϕ) was <0.5. In contrast, the IL-6/Mϕ was greater than 0.5 at ≥7 days after the IVC ligation. These observations implied that IL-6/Mϕ ratios of >0.5 would strongly indicate thrombus ages of ≥7 days. Reciprocally, the ratios of less than 0.5 would suggest thrombus ages of ≤5 days. The present study demonstrated that the immunohistochemical detection of IL-6 was suitable to estimate the age of venous thrombi. Forensic pathology (dpeaa)DE-He213 Thrombus age estimation (dpeaa)DE-He213 Immunohistochemistry (dpeaa)DE-He213 IL-6 (dpeaa)DE-He213 Macrophages (dpeaa)DE-He213 Ishida, Yuko verfasserin aut Kimura, Akihiko verfasserin aut Hama, Mizuki verfasserin aut Kawaguchi, Takashi verfasserin aut Yamamoto, Hiroki verfasserin aut Kuninaka, Yumi verfasserin aut Shimada, Emi verfasserin aut Kondo, Toshikazu verfasserin aut Enthalten in International journal of legal medicine Berlin : Springer, 1922 129(2015), 5 vom: 24. Jan., Seite 1021-1025 (DE-627)253724295 (DE-600)1459222-8 1437-1596 nnns volume:129 year:2015 number:5 day:24 month:01 pages:1021-1025 https://dx.doi.org/10.1007/s00414-015-1147-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_711 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.00 ASE 86.00 ASE 44.72 ASE AR 129 2015 5 24 01 1021-1025
allfields_unstemmed	10.1007/s00414-015-1147-9 doi (DE-627)SPR005300487 (SPR)s00414-015-1147-9-e DE-627 ger DE-627 rakwb eng 340 610 ASE 44.00 bkl 86.00 bkl 44.72 bkl Nosaka, Mizuho verfasserin aut Immunohistochemical detection of intrathrombotic IL-6 and its application to thrombus age estimation 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract We immunohistochemically examined intrathrombotic IL-6 expression using a murine model of deep vein thrombosis induced by the ligation of the inferior vena cava (IVC) and discussed the availability of intrathrombotic IL-6 for thrombus age estimation. IL-$ 6^{+} $ cells could be first detected at 1 day after IVC ligation in three of five samples, and all samples with postligation intervals of 3 days or more had intrathrombotic IL-$ 6^{+} $ cells. Thereafter, the numbers of IL-$ 6^{+} $ cells were elevated with the increase of postligation intervals. Double-color immunofluorescence analyses demonstrated that IL-6 was mainly expressed by intrathrombotic macrophages. In all samples with postligation intervals of 5 days or less, the IL-6/macrophage ratio (IL-6/Mϕ) was <0.5. In contrast, the IL-6/Mϕ was greater than 0.5 at ≥7 days after the IVC ligation. These observations implied that IL-6/Mϕ ratios of >0.5 would strongly indicate thrombus ages of ≥7 days. Reciprocally, the ratios of less than 0.5 would suggest thrombus ages of ≤5 days. The present study demonstrated that the immunohistochemical detection of IL-6 was suitable to estimate the age of venous thrombi. Forensic pathology (dpeaa)DE-He213 Thrombus age estimation (dpeaa)DE-He213 Immunohistochemistry (dpeaa)DE-He213 IL-6 (dpeaa)DE-He213 Macrophages (dpeaa)DE-He213 Ishida, Yuko verfasserin aut Kimura, Akihiko verfasserin aut Hama, Mizuki verfasserin aut Kawaguchi, Takashi verfasserin aut Yamamoto, Hiroki verfasserin aut Kuninaka, Yumi verfasserin aut Shimada, Emi verfasserin aut Kondo, Toshikazu verfasserin aut Enthalten in International journal of legal medicine Berlin : Springer, 1922 129(2015), 5 vom: 24. Jan., Seite 1021-1025 (DE-627)253724295 (DE-600)1459222-8 1437-1596 nnns volume:129 year:2015 number:5 day:24 month:01 pages:1021-1025 https://dx.doi.org/10.1007/s00414-015-1147-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_711 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.00 ASE 86.00 ASE 44.72 ASE AR 129 2015 5 24 01 1021-1025
allfieldsGer	10.1007/s00414-015-1147-9 doi (DE-627)SPR005300487 (SPR)s00414-015-1147-9-e DE-627 ger DE-627 rakwb eng 340 610 ASE 44.00 bkl 86.00 bkl 44.72 bkl Nosaka, Mizuho verfasserin aut Immunohistochemical detection of intrathrombotic IL-6 and its application to thrombus age estimation 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract We immunohistochemically examined intrathrombotic IL-6 expression using a murine model of deep vein thrombosis induced by the ligation of the inferior vena cava (IVC) and discussed the availability of intrathrombotic IL-6 for thrombus age estimation. IL-$ 6^{+} $ cells could be first detected at 1 day after IVC ligation in three of five samples, and all samples with postligation intervals of 3 days or more had intrathrombotic IL-$ 6^{+} $ cells. Thereafter, the numbers of IL-$ 6^{+} $ cells were elevated with the increase of postligation intervals. Double-color immunofluorescence analyses demonstrated that IL-6 was mainly expressed by intrathrombotic macrophages. In all samples with postligation intervals of 5 days or less, the IL-6/macrophage ratio (IL-6/Mϕ) was <0.5. In contrast, the IL-6/Mϕ was greater than 0.5 at ≥7 days after the IVC ligation. These observations implied that IL-6/Mϕ ratios of >0.5 would strongly indicate thrombus ages of ≥7 days. Reciprocally, the ratios of less than 0.5 would suggest thrombus ages of ≤5 days. The present study demonstrated that the immunohistochemical detection of IL-6 was suitable to estimate the age of venous thrombi. Forensic pathology (dpeaa)DE-He213 Thrombus age estimation (dpeaa)DE-He213 Immunohistochemistry (dpeaa)DE-He213 IL-6 (dpeaa)DE-He213 Macrophages (dpeaa)DE-He213 Ishida, Yuko verfasserin aut Kimura, Akihiko verfasserin aut Hama, Mizuki verfasserin aut Kawaguchi, Takashi verfasserin aut Yamamoto, Hiroki verfasserin aut Kuninaka, Yumi verfasserin aut Shimada, Emi verfasserin aut Kondo, Toshikazu verfasserin aut Enthalten in International journal of legal medicine Berlin : Springer, 1922 129(2015), 5 vom: 24. Jan., Seite 1021-1025 (DE-627)253724295 (DE-600)1459222-8 1437-1596 nnns volume:129 year:2015 number:5 day:24 month:01 pages:1021-1025 https://dx.doi.org/10.1007/s00414-015-1147-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_711 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.00 ASE 86.00 ASE 44.72 ASE AR 129 2015 5 24 01 1021-1025
allfieldsSound	10.1007/s00414-015-1147-9 doi (DE-627)SPR005300487 (SPR)s00414-015-1147-9-e DE-627 ger DE-627 rakwb eng 340 610 ASE 44.00 bkl 86.00 bkl 44.72 bkl Nosaka, Mizuho verfasserin aut Immunohistochemical detection of intrathrombotic IL-6 and its application to thrombus age estimation 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract We immunohistochemically examined intrathrombotic IL-6 expression using a murine model of deep vein thrombosis induced by the ligation of the inferior vena cava (IVC) and discussed the availability of intrathrombotic IL-6 for thrombus age estimation. IL-$ 6^{+} $ cells could be first detected at 1 day after IVC ligation in three of five samples, and all samples with postligation intervals of 3 days or more had intrathrombotic IL-$ 6^{+} $ cells. Thereafter, the numbers of IL-$ 6^{+} $ cells were elevated with the increase of postligation intervals. Double-color immunofluorescence analyses demonstrated that IL-6 was mainly expressed by intrathrombotic macrophages. In all samples with postligation intervals of 5 days or less, the IL-6/macrophage ratio (IL-6/Mϕ) was <0.5. In contrast, the IL-6/Mϕ was greater than 0.5 at ≥7 days after the IVC ligation. These observations implied that IL-6/Mϕ ratios of >0.5 would strongly indicate thrombus ages of ≥7 days. Reciprocally, the ratios of less than 0.5 would suggest thrombus ages of ≤5 days. The present study demonstrated that the immunohistochemical detection of IL-6 was suitable to estimate the age of venous thrombi. Forensic pathology (dpeaa)DE-He213 Thrombus age estimation (dpeaa)DE-He213 Immunohistochemistry (dpeaa)DE-He213 IL-6 (dpeaa)DE-He213 Macrophages (dpeaa)DE-He213 Ishida, Yuko verfasserin aut Kimura, Akihiko verfasserin aut Hama, Mizuki verfasserin aut Kawaguchi, Takashi verfasserin aut Yamamoto, Hiroki verfasserin aut Kuninaka, Yumi verfasserin aut Shimada, Emi verfasserin aut Kondo, Toshikazu verfasserin aut Enthalten in International journal of legal medicine Berlin : Springer, 1922 129(2015), 5 vom: 24. Jan., Seite 1021-1025 (DE-627)253724295 (DE-600)1459222-8 1437-1596 nnns volume:129 year:2015 number:5 day:24 month:01 pages:1021-1025 https://dx.doi.org/10.1007/s00414-015-1147-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_711 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.00 ASE 86.00 ASE 44.72 ASE AR 129 2015 5 24 01 1021-1025
language	English
source	Enthalten in International journal of legal medicine 129(2015), 5 vom: 24. Jan., Seite 1021-1025 volume:129 year:2015 number:5 day:24 month:01 pages:1021-1025
sourceStr	Enthalten in International journal of legal medicine 129(2015), 5 vom: 24. Jan., Seite 1021-1025 volume:129 year:2015 number:5 day:24 month:01 pages:1021-1025
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Forensic pathology Thrombus age estimation Immunohistochemistry IL-6 Macrophages
dewey-raw	340
isfreeaccess_bool	false
container_title	International journal of legal medicine
authorswithroles_txt_mv	Nosaka, Mizuho @@aut@@ Ishida, Yuko @@aut@@ Kimura, Akihiko @@aut@@ Hama, Mizuki @@aut@@ Kawaguchi, Takashi @@aut@@ Yamamoto, Hiroki @@aut@@ Kuninaka, Yumi @@aut@@ Shimada, Emi @@aut@@ Kondo, Toshikazu @@aut@@
publishDateDaySort_date	2015-01-24T00:00:00Z
hierarchy_top_id	253724295
dewey-sort	3340
id	SPR005300487
language_de	englisch
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author	Nosaka, Mizuho
spellingShingle	Nosaka, Mizuho ddc 340 bkl 44.00 bkl 86.00 bkl 44.72 misc Forensic pathology misc Thrombus age estimation misc Immunohistochemistry misc IL-6 misc Macrophages Immunohistochemical detection of intrathrombotic IL-6 and its application to thrombus age estimation
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topic_title	340 610 ASE 44.00 bkl 86.00 bkl 44.72 bkl Immunohistochemical detection of intrathrombotic IL-6 and its application to thrombus age estimation Forensic pathology (dpeaa)DE-He213 Thrombus age estimation (dpeaa)DE-He213 Immunohistochemistry (dpeaa)DE-He213 IL-6 (dpeaa)DE-He213 Macrophages (dpeaa)DE-He213
topic	ddc 340 bkl 44.00 bkl 86.00 bkl 44.72 misc Forensic pathology misc Thrombus age estimation misc Immunohistochemistry misc IL-6 misc Macrophages
topic_unstemmed	ddc 340 bkl 44.00 bkl 86.00 bkl 44.72 misc Forensic pathology misc Thrombus age estimation misc Immunohistochemistry misc IL-6 misc Macrophages
topic_browse	ddc 340 bkl 44.00 bkl 86.00 bkl 44.72 misc Forensic pathology misc Thrombus age estimation misc Immunohistochemistry misc IL-6 misc Macrophages
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title	Immunohistochemical detection of intrathrombotic IL-6 and its application to thrombus age estimation
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title_full	Immunohistochemical detection of intrathrombotic IL-6 and its application to thrombus age estimation
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author_browse	Nosaka, Mizuho Ishida, Yuko Kimura, Akihiko Hama, Mizuki Kawaguchi, Takashi Yamamoto, Hiroki Kuninaka, Yumi Shimada, Emi Kondo, Toshikazu
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title_sort	immunohistochemical detection of intrathrombotic il-6 and its application to thrombus age estimation
title_auth	Immunohistochemical detection of intrathrombotic IL-6 and its application to thrombus age estimation
abstract	Abstract We immunohistochemically examined intrathrombotic IL-6 expression using a murine model of deep vein thrombosis induced by the ligation of the inferior vena cava (IVC) and discussed the availability of intrathrombotic IL-6 for thrombus age estimation. IL-$ 6^{+} $ cells could be first detected at 1 day after IVC ligation in three of five samples, and all samples with postligation intervals of 3 days or more had intrathrombotic IL-$ 6^{+} $ cells. Thereafter, the numbers of IL-$ 6^{+} $ cells were elevated with the increase of postligation intervals. Double-color immunofluorescence analyses demonstrated that IL-6 was mainly expressed by intrathrombotic macrophages. In all samples with postligation intervals of 5 days or less, the IL-6/macrophage ratio (IL-6/Mϕ) was <0.5. In contrast, the IL-6/Mϕ was greater than 0.5 at ≥7 days after the IVC ligation. These observations implied that IL-6/Mϕ ratios of >0.5 would strongly indicate thrombus ages of ≥7 days. Reciprocally, the ratios of less than 0.5 would suggest thrombus ages of ≤5 days. The present study demonstrated that the immunohistochemical detection of IL-6 was suitable to estimate the age of venous thrombi.
abstractGer	Abstract We immunohistochemically examined intrathrombotic IL-6 expression using a murine model of deep vein thrombosis induced by the ligation of the inferior vena cava (IVC) and discussed the availability of intrathrombotic IL-6 for thrombus age estimation. IL-$ 6^{+} $ cells could be first detected at 1 day after IVC ligation in three of five samples, and all samples with postligation intervals of 3 days or more had intrathrombotic IL-$ 6^{+} $ cells. Thereafter, the numbers of IL-$ 6^{+} $ cells were elevated with the increase of postligation intervals. Double-color immunofluorescence analyses demonstrated that IL-6 was mainly expressed by intrathrombotic macrophages. In all samples with postligation intervals of 5 days or less, the IL-6/macrophage ratio (IL-6/Mϕ) was <0.5. In contrast, the IL-6/Mϕ was greater than 0.5 at ≥7 days after the IVC ligation. These observations implied that IL-6/Mϕ ratios of >0.5 would strongly indicate thrombus ages of ≥7 days. Reciprocally, the ratios of less than 0.5 would suggest thrombus ages of ≤5 days. The present study demonstrated that the immunohistochemical detection of IL-6 was suitable to estimate the age of venous thrombi.
abstract_unstemmed	Abstract We immunohistochemically examined intrathrombotic IL-6 expression using a murine model of deep vein thrombosis induced by the ligation of the inferior vena cava (IVC) and discussed the availability of intrathrombotic IL-6 for thrombus age estimation. IL-$ 6^{+} $ cells could be first detected at 1 day after IVC ligation in three of five samples, and all samples with postligation intervals of 3 days or more had intrathrombotic IL-$ 6^{+} $ cells. Thereafter, the numbers of IL-$ 6^{+} $ cells were elevated with the increase of postligation intervals. Double-color immunofluorescence analyses demonstrated that IL-6 was mainly expressed by intrathrombotic macrophages. In all samples with postligation intervals of 5 days or less, the IL-6/macrophage ratio (IL-6/Mϕ) was <0.5. In contrast, the IL-6/Mϕ was greater than 0.5 at ≥7 days after the IVC ligation. These observations implied that IL-6/Mϕ ratios of >0.5 would strongly indicate thrombus ages of ≥7 days. Reciprocally, the ratios of less than 0.5 would suggest thrombus ages of ≤5 days. The present study demonstrated that the immunohistochemical detection of IL-6 was suitable to estimate the age of venous thrombi.
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container_issue	5
title_short	Immunohistochemical detection of intrathrombotic IL-6 and its application to thrombus age estimation
url	https://dx.doi.org/10.1007/s00414-015-1147-9
remote_bool	true
author2	Ishida, Yuko Kimura, Akihiko Hama, Mizuki Kawaguchi, Takashi Yamamoto, Hiroki Kuninaka, Yumi Shimada, Emi Kondo, Toshikazu
author2Str	Ishida, Yuko Kimura, Akihiko Hama, Mizuki Kawaguchi, Takashi Yamamoto, Hiroki Kuninaka, Yumi Shimada, Emi Kondo, Toshikazu
ppnlink	253724295
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doi_str	10.1007/s00414-015-1147-9
up_date	2024-07-03T15:22:28.073Z
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Immunohistochemical detection of intrathrombotic IL-6 and its application to thrombus age estimation

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