Immunohistochemical analysis on MMP-2 and MMP-9 for wound age determination
Abstract We performed immunohistochemical study combined with morphometrical analyses in order to examine the expression of matrix metalloproteinase-2 (MMP-2) and MMP-9 using 55 human skin wounds of different ages: group I, 0–3 days (n = 16); II, 4–7 days (n = 11); III, 9–14 days (n = 16); and IV, 1...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Ishida, Yuko [verfasserIn] Kuninaka, Yumi [verfasserIn] Nosaka, Mizuho [verfasserIn] Kimura, Akihiko [verfasserIn] Kawaguchi, Takashi [verfasserIn] Hama, Mizuki [verfasserIn] Sakamoto, Shoichi [verfasserIn] Shinozaki, Kouhei [verfasserIn] Eisenmenger, Wolfgang [verfasserIn] Kondo, Toshikazu [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2015 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: International journal of legal medicine - Berlin : Springer, 1922, 129(2015), 5 vom: 10. März, Seite 1043-1048 |
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| Übergeordnetes Werk: |
volume:129 ; year:2015 ; number:5 ; day:10 ; month:03 ; pages:1043-1048 |
| Links: |
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| DOI / URN: |
10.1007/s00414-015-1167-5 |
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| Katalog-ID: |
SPR005300584 |
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| 100 | 1 | |a Ishida, Yuko |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Immunohistochemical analysis on MMP-2 and MMP-9 for wound age determination |
| 264 | 1 | |c 2015 | |
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| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 520 | |a Abstract We performed immunohistochemical study combined with morphometrical analyses in order to examine the expression of matrix metalloproteinase-2 (MMP-2) and MMP-9 using 55 human skin wounds of different ages: group I, 0–3 days (n = 16); II, 4–7 days (n = 11); III, 9–14 days (n = 16); and IV, 17–21 days (n = 12). Immunopositive reactions for MMP-2 were observed in all human skin specimens including uninjured skin as control. The number of MMP-$ 2^{+} $ macrophages was significantly increased in accordance with wound ages. In contrast to MMP-2, no MMP-$ 9^{+} $ signals were detected in uninjured and wound specimens aged less than 1 day. However, the number of MMP-$ 9^{+} $ macrophages profoundly appeared in groups II and III. Morphometrically, in all of wound samples aged 9–12 days, MMP-$ 2^{+} $ cell number was more than 20. On the contrary, most of the remaining samples had <20 positive cells. However, only one sample (a 7-day-old wound) showed 21 positive cells. Thus, with regard to practical applicability with forensic safety, MMP-$ 2^{+} $ macrophages of >20 would indicate a wound age of 7–12 days. Additionally, 10 out of 12 wound specimens aged 9–12 days showed the MMP-$ 2^{+} $ cell number of >25, implying that MMP-$ 2^{+} $ cell number of >25 would indicate the wound age of 9–12 days. On the contrary, all wound samples aged 3–14 days except for only one sample had MMP-$ 9^{+} $ cell number of >30, indicating that MMP-$ 9^{+} $ cell number of >30 would indicate the wound age of 3–14 days. Collectively, MMP-2 seemed to be more distinct marker, compared with MMP-9. | ||
| 650 | 4 | |a Forensic pathology |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a MMP-2 |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a MMP-9 |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Wound age determination |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Immunohistochemistry |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Kuninaka, Yumi |e verfasserin |4 aut | |
| 700 | 1 | |a Nosaka, Mizuho |e verfasserin |4 aut | |
| 700 | 1 | |a Kimura, Akihiko |e verfasserin |4 aut | |
| 700 | 1 | |a Kawaguchi, Takashi |e verfasserin |4 aut | |
| 700 | 1 | |a Hama, Mizuki |e verfasserin |4 aut | |
| 700 | 1 | |a Sakamoto, Shoichi |e verfasserin |4 aut | |
| 700 | 1 | |a Shinozaki, Kouhei |e verfasserin |4 aut | |
| 700 | 1 | |a Eisenmenger, Wolfgang |e verfasserin |4 aut | |
| 700 | 1 | |a Kondo, Toshikazu |e verfasserin |4 aut | |
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| 773 | 1 | 8 | |g volume:129 |g year:2015 |g number:5 |g day:10 |g month:03 |g pages:1043-1048 |
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10.1007/s00414-015-1167-5 doi (DE-627)SPR005300584 (SPR)s00414-015-1167-5-e DE-627 ger DE-627 rakwb eng 340 610 ASE 44.00 bkl 86.00 bkl 44.72 bkl Ishida, Yuko verfasserin aut Immunohistochemical analysis on MMP-2 and MMP-9 for wound age determination 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract We performed immunohistochemical study combined with morphometrical analyses in order to examine the expression of matrix metalloproteinase-2 (MMP-2) and MMP-9 using 55 human skin wounds of different ages: group I, 0–3 days (n = 16); II, 4–7 days (n = 11); III, 9–14 days (n = 16); and IV, 17–21 days (n = 12). Immunopositive reactions for MMP-2 were observed in all human skin specimens including uninjured skin as control. The number of MMP-$ 2^{+} $ macrophages was significantly increased in accordance with wound ages. In contrast to MMP-2, no MMP-$ 9^{+} $ signals were detected in uninjured and wound specimens aged less than 1 day. However, the number of MMP-$ 9^{+} $ macrophages profoundly appeared in groups II and III. Morphometrically, in all of wound samples aged 9–12 days, MMP-$ 2^{+} $ cell number was more than 20. On the contrary, most of the remaining samples had <20 positive cells. However, only one sample (a 7-day-old wound) showed 21 positive cells. Thus, with regard to practical applicability with forensic safety, MMP-$ 2^{+} $ macrophages of >20 would indicate a wound age of 7–12 days. Additionally, 10 out of 12 wound specimens aged 9–12 days showed the MMP-$ 2^{+} $ cell number of >25, implying that MMP-$ 2^{+} $ cell number of >25 would indicate the wound age of 9–12 days. On the contrary, all wound samples aged 3–14 days except for only one sample had MMP-$ 9^{+} $ cell number of >30, indicating that MMP-$ 9^{+} $ cell number of >30 would indicate the wound age of 3–14 days. Collectively, MMP-2 seemed to be more distinct marker, compared with MMP-9. Forensic pathology (dpeaa)DE-He213 MMP-2 (dpeaa)DE-He213 MMP-9 (dpeaa)DE-He213 Wound age determination (dpeaa)DE-He213 Immunohistochemistry (dpeaa)DE-He213 Kuninaka, Yumi verfasserin aut Nosaka, Mizuho verfasserin aut Kimura, Akihiko verfasserin aut Kawaguchi, Takashi verfasserin aut Hama, Mizuki verfasserin aut Sakamoto, Shoichi verfasserin aut Shinozaki, Kouhei verfasserin aut Eisenmenger, Wolfgang verfasserin aut Kondo, Toshikazu verfasserin aut Enthalten in International journal of legal medicine Berlin : Springer, 1922 129(2015), 5 vom: 10. März, Seite 1043-1048 (DE-627)253724295 (DE-600)1459222-8 1437-1596 nnns volume:129 year:2015 number:5 day:10 month:03 pages:1043-1048 https://dx.doi.org/10.1007/s00414-015-1167-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_711 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.00 ASE 86.00 ASE 44.72 ASE AR 129 2015 5 10 03 1043-1048 |
| spelling |
10.1007/s00414-015-1167-5 doi (DE-627)SPR005300584 (SPR)s00414-015-1167-5-e DE-627 ger DE-627 rakwb eng 340 610 ASE 44.00 bkl 86.00 bkl 44.72 bkl Ishida, Yuko verfasserin aut Immunohistochemical analysis on MMP-2 and MMP-9 for wound age determination 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract We performed immunohistochemical study combined with morphometrical analyses in order to examine the expression of matrix metalloproteinase-2 (MMP-2) and MMP-9 using 55 human skin wounds of different ages: group I, 0–3 days (n = 16); II, 4–7 days (n = 11); III, 9–14 days (n = 16); and IV, 17–21 days (n = 12). Immunopositive reactions for MMP-2 were observed in all human skin specimens including uninjured skin as control. The number of MMP-$ 2^{+} $ macrophages was significantly increased in accordance with wound ages. In contrast to MMP-2, no MMP-$ 9^{+} $ signals were detected in uninjured and wound specimens aged less than 1 day. However, the number of MMP-$ 9^{+} $ macrophages profoundly appeared in groups II and III. Morphometrically, in all of wound samples aged 9–12 days, MMP-$ 2^{+} $ cell number was more than 20. On the contrary, most of the remaining samples had <20 positive cells. However, only one sample (a 7-day-old wound) showed 21 positive cells. Thus, with regard to practical applicability with forensic safety, MMP-$ 2^{+} $ macrophages of >20 would indicate a wound age of 7–12 days. Additionally, 10 out of 12 wound specimens aged 9–12 days showed the MMP-$ 2^{+} $ cell number of >25, implying that MMP-$ 2^{+} $ cell number of >25 would indicate the wound age of 9–12 days. On the contrary, all wound samples aged 3–14 days except for only one sample had MMP-$ 9^{+} $ cell number of >30, indicating that MMP-$ 9^{+} $ cell number of >30 would indicate the wound age of 3–14 days. Collectively, MMP-2 seemed to be more distinct marker, compared with MMP-9. Forensic pathology (dpeaa)DE-He213 MMP-2 (dpeaa)DE-He213 MMP-9 (dpeaa)DE-He213 Wound age determination (dpeaa)DE-He213 Immunohistochemistry (dpeaa)DE-He213 Kuninaka, Yumi verfasserin aut Nosaka, Mizuho verfasserin aut Kimura, Akihiko verfasserin aut Kawaguchi, Takashi verfasserin aut Hama, Mizuki verfasserin aut Sakamoto, Shoichi verfasserin aut Shinozaki, Kouhei verfasserin aut Eisenmenger, Wolfgang verfasserin aut Kondo, Toshikazu verfasserin aut Enthalten in International journal of legal medicine Berlin : Springer, 1922 129(2015), 5 vom: 10. März, Seite 1043-1048 (DE-627)253724295 (DE-600)1459222-8 1437-1596 nnns volume:129 year:2015 number:5 day:10 month:03 pages:1043-1048 https://dx.doi.org/10.1007/s00414-015-1167-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_711 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.00 ASE 86.00 ASE 44.72 ASE AR 129 2015 5 10 03 1043-1048 |
| allfields_unstemmed |
10.1007/s00414-015-1167-5 doi (DE-627)SPR005300584 (SPR)s00414-015-1167-5-e DE-627 ger DE-627 rakwb eng 340 610 ASE 44.00 bkl 86.00 bkl 44.72 bkl Ishida, Yuko verfasserin aut Immunohistochemical analysis on MMP-2 and MMP-9 for wound age determination 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract We performed immunohistochemical study combined with morphometrical analyses in order to examine the expression of matrix metalloproteinase-2 (MMP-2) and MMP-9 using 55 human skin wounds of different ages: group I, 0–3 days (n = 16); II, 4–7 days (n = 11); III, 9–14 days (n = 16); and IV, 17–21 days (n = 12). Immunopositive reactions for MMP-2 were observed in all human skin specimens including uninjured skin as control. The number of MMP-$ 2^{+} $ macrophages was significantly increased in accordance with wound ages. In contrast to MMP-2, no MMP-$ 9^{+} $ signals were detected in uninjured and wound specimens aged less than 1 day. However, the number of MMP-$ 9^{+} $ macrophages profoundly appeared in groups II and III. Morphometrically, in all of wound samples aged 9–12 days, MMP-$ 2^{+} $ cell number was more than 20. On the contrary, most of the remaining samples had <20 positive cells. However, only one sample (a 7-day-old wound) showed 21 positive cells. Thus, with regard to practical applicability with forensic safety, MMP-$ 2^{+} $ macrophages of >20 would indicate a wound age of 7–12 days. Additionally, 10 out of 12 wound specimens aged 9–12 days showed the MMP-$ 2^{+} $ cell number of >25, implying that MMP-$ 2^{+} $ cell number of >25 would indicate the wound age of 9–12 days. On the contrary, all wound samples aged 3–14 days except for only one sample had MMP-$ 9^{+} $ cell number of >30, indicating that MMP-$ 9^{+} $ cell number of >30 would indicate the wound age of 3–14 days. Collectively, MMP-2 seemed to be more distinct marker, compared with MMP-9. Forensic pathology (dpeaa)DE-He213 MMP-2 (dpeaa)DE-He213 MMP-9 (dpeaa)DE-He213 Wound age determination (dpeaa)DE-He213 Immunohistochemistry (dpeaa)DE-He213 Kuninaka, Yumi verfasserin aut Nosaka, Mizuho verfasserin aut Kimura, Akihiko verfasserin aut Kawaguchi, Takashi verfasserin aut Hama, Mizuki verfasserin aut Sakamoto, Shoichi verfasserin aut Shinozaki, Kouhei verfasserin aut Eisenmenger, Wolfgang verfasserin aut Kondo, Toshikazu verfasserin aut Enthalten in International journal of legal medicine Berlin : Springer, 1922 129(2015), 5 vom: 10. März, Seite 1043-1048 (DE-627)253724295 (DE-600)1459222-8 1437-1596 nnns volume:129 year:2015 number:5 day:10 month:03 pages:1043-1048 https://dx.doi.org/10.1007/s00414-015-1167-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_711 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.00 ASE 86.00 ASE 44.72 ASE AR 129 2015 5 10 03 1043-1048 |
| allfieldsGer |
10.1007/s00414-015-1167-5 doi (DE-627)SPR005300584 (SPR)s00414-015-1167-5-e DE-627 ger DE-627 rakwb eng 340 610 ASE 44.00 bkl 86.00 bkl 44.72 bkl Ishida, Yuko verfasserin aut Immunohistochemical analysis on MMP-2 and MMP-9 for wound age determination 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract We performed immunohistochemical study combined with morphometrical analyses in order to examine the expression of matrix metalloproteinase-2 (MMP-2) and MMP-9 using 55 human skin wounds of different ages: group I, 0–3 days (n = 16); II, 4–7 days (n = 11); III, 9–14 days (n = 16); and IV, 17–21 days (n = 12). Immunopositive reactions for MMP-2 were observed in all human skin specimens including uninjured skin as control. The number of MMP-$ 2^{+} $ macrophages was significantly increased in accordance with wound ages. In contrast to MMP-2, no MMP-$ 9^{+} $ signals were detected in uninjured and wound specimens aged less than 1 day. However, the number of MMP-$ 9^{+} $ macrophages profoundly appeared in groups II and III. Morphometrically, in all of wound samples aged 9–12 days, MMP-$ 2^{+} $ cell number was more than 20. On the contrary, most of the remaining samples had <20 positive cells. However, only one sample (a 7-day-old wound) showed 21 positive cells. Thus, with regard to practical applicability with forensic safety, MMP-$ 2^{+} $ macrophages of >20 would indicate a wound age of 7–12 days. Additionally, 10 out of 12 wound specimens aged 9–12 days showed the MMP-$ 2^{+} $ cell number of >25, implying that MMP-$ 2^{+} $ cell number of >25 would indicate the wound age of 9–12 days. On the contrary, all wound samples aged 3–14 days except for only one sample had MMP-$ 9^{+} $ cell number of >30, indicating that MMP-$ 9^{+} $ cell number of >30 would indicate the wound age of 3–14 days. Collectively, MMP-2 seemed to be more distinct marker, compared with MMP-9. Forensic pathology (dpeaa)DE-He213 MMP-2 (dpeaa)DE-He213 MMP-9 (dpeaa)DE-He213 Wound age determination (dpeaa)DE-He213 Immunohistochemistry (dpeaa)DE-He213 Kuninaka, Yumi verfasserin aut Nosaka, Mizuho verfasserin aut Kimura, Akihiko verfasserin aut Kawaguchi, Takashi verfasserin aut Hama, Mizuki verfasserin aut Sakamoto, Shoichi verfasserin aut Shinozaki, Kouhei verfasserin aut Eisenmenger, Wolfgang verfasserin aut Kondo, Toshikazu verfasserin aut Enthalten in International journal of legal medicine Berlin : Springer, 1922 129(2015), 5 vom: 10. März, Seite 1043-1048 (DE-627)253724295 (DE-600)1459222-8 1437-1596 nnns volume:129 year:2015 number:5 day:10 month:03 pages:1043-1048 https://dx.doi.org/10.1007/s00414-015-1167-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_711 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.00 ASE 86.00 ASE 44.72 ASE AR 129 2015 5 10 03 1043-1048 |
| allfieldsSound |
10.1007/s00414-015-1167-5 doi (DE-627)SPR005300584 (SPR)s00414-015-1167-5-e DE-627 ger DE-627 rakwb eng 340 610 ASE 44.00 bkl 86.00 bkl 44.72 bkl Ishida, Yuko verfasserin aut Immunohistochemical analysis on MMP-2 and MMP-9 for wound age determination 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract We performed immunohistochemical study combined with morphometrical analyses in order to examine the expression of matrix metalloproteinase-2 (MMP-2) and MMP-9 using 55 human skin wounds of different ages: group I, 0–3 days (n = 16); II, 4–7 days (n = 11); III, 9–14 days (n = 16); and IV, 17–21 days (n = 12). Immunopositive reactions for MMP-2 were observed in all human skin specimens including uninjured skin as control. The number of MMP-$ 2^{+} $ macrophages was significantly increased in accordance with wound ages. In contrast to MMP-2, no MMP-$ 9^{+} $ signals were detected in uninjured and wound specimens aged less than 1 day. However, the number of MMP-$ 9^{+} $ macrophages profoundly appeared in groups II and III. Morphometrically, in all of wound samples aged 9–12 days, MMP-$ 2^{+} $ cell number was more than 20. On the contrary, most of the remaining samples had <20 positive cells. However, only one sample (a 7-day-old wound) showed 21 positive cells. Thus, with regard to practical applicability with forensic safety, MMP-$ 2^{+} $ macrophages of >20 would indicate a wound age of 7–12 days. Additionally, 10 out of 12 wound specimens aged 9–12 days showed the MMP-$ 2^{+} $ cell number of >25, implying that MMP-$ 2^{+} $ cell number of >25 would indicate the wound age of 9–12 days. On the contrary, all wound samples aged 3–14 days except for only one sample had MMP-$ 9^{+} $ cell number of >30, indicating that MMP-$ 9^{+} $ cell number of >30 would indicate the wound age of 3–14 days. Collectively, MMP-2 seemed to be more distinct marker, compared with MMP-9. Forensic pathology (dpeaa)DE-He213 MMP-2 (dpeaa)DE-He213 MMP-9 (dpeaa)DE-He213 Wound age determination (dpeaa)DE-He213 Immunohistochemistry (dpeaa)DE-He213 Kuninaka, Yumi verfasserin aut Nosaka, Mizuho verfasserin aut Kimura, Akihiko verfasserin aut Kawaguchi, Takashi verfasserin aut Hama, Mizuki verfasserin aut Sakamoto, Shoichi verfasserin aut Shinozaki, Kouhei verfasserin aut Eisenmenger, Wolfgang verfasserin aut Kondo, Toshikazu verfasserin aut Enthalten in International journal of legal medicine Berlin : Springer, 1922 129(2015), 5 vom: 10. März, Seite 1043-1048 (DE-627)253724295 (DE-600)1459222-8 1437-1596 nnns volume:129 year:2015 number:5 day:10 month:03 pages:1043-1048 https://dx.doi.org/10.1007/s00414-015-1167-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_711 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.00 ASE 86.00 ASE 44.72 ASE AR 129 2015 5 10 03 1043-1048 |
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Enthalten in International journal of legal medicine 129(2015), 5 vom: 10. März, Seite 1043-1048 volume:129 year:2015 number:5 day:10 month:03 pages:1043-1048 |
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Forensic pathology MMP-2 MMP-9 Wound age determination Immunohistochemistry |
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International journal of legal medicine |
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Ishida, Yuko @@aut@@ Kuninaka, Yumi @@aut@@ Nosaka, Mizuho @@aut@@ Kimura, Akihiko @@aut@@ Kawaguchi, Takashi @@aut@@ Hama, Mizuki @@aut@@ Sakamoto, Shoichi @@aut@@ Shinozaki, Kouhei @@aut@@ Eisenmenger, Wolfgang @@aut@@ Kondo, Toshikazu @@aut@@ |
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2015-03-10T00:00:00Z |
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| author |
Ishida, Yuko |
| spellingShingle |
Ishida, Yuko ddc 340 bkl 44.00 bkl 86.00 bkl 44.72 misc Forensic pathology misc MMP-2 misc MMP-9 misc Wound age determination misc Immunohistochemistry Immunohistochemical analysis on MMP-2 and MMP-9 for wound age determination |
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1437-1596 |
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340 610 ASE 44.00 bkl 86.00 bkl 44.72 bkl Immunohistochemical analysis on MMP-2 and MMP-9 for wound age determination Forensic pathology (dpeaa)DE-He213 MMP-2 (dpeaa)DE-He213 MMP-9 (dpeaa)DE-He213 Wound age determination (dpeaa)DE-He213 Immunohistochemistry (dpeaa)DE-He213 |
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ddc 340 bkl 44.00 bkl 86.00 bkl 44.72 misc Forensic pathology misc MMP-2 misc MMP-9 misc Wound age determination misc Immunohistochemistry |
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ddc 340 bkl 44.00 bkl 86.00 bkl 44.72 misc Forensic pathology misc MMP-2 misc MMP-9 misc Wound age determination misc Immunohistochemistry |
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ddc 340 bkl 44.00 bkl 86.00 bkl 44.72 misc Forensic pathology misc MMP-2 misc MMP-9 misc Wound age determination misc Immunohistochemistry |
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Elektronische Aufsätze Aufsätze Elektronische Ressource |
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International journal of legal medicine |
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International journal of legal medicine |
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Immunohistochemical analysis on MMP-2 and MMP-9 for wound age determination |
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(DE-627)SPR005300584 (SPR)s00414-015-1167-5-e |
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Immunohistochemical analysis on MMP-2 and MMP-9 for wound age determination |
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Ishida, Yuko |
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International journal of legal medicine |
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International journal of legal medicine |
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eng |
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Ishida, Yuko Kuninaka, Yumi Nosaka, Mizuho Kimura, Akihiko Kawaguchi, Takashi Hama, Mizuki Sakamoto, Shoichi Shinozaki, Kouhei Eisenmenger, Wolfgang Kondo, Toshikazu |
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Elektronische Aufsätze |
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Ishida, Yuko |
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10.1007/s00414-015-1167-5 |
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340 610 |
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verfasserin |
| title_sort |
immunohistochemical analysis on mmp-2 and mmp-9 for wound age determination |
| title_auth |
Immunohistochemical analysis on MMP-2 and MMP-9 for wound age determination |
| abstract |
Abstract We performed immunohistochemical study combined with morphometrical analyses in order to examine the expression of matrix metalloproteinase-2 (MMP-2) and MMP-9 using 55 human skin wounds of different ages: group I, 0–3 days (n = 16); II, 4–7 days (n = 11); III, 9–14 days (n = 16); and IV, 17–21 days (n = 12). Immunopositive reactions for MMP-2 were observed in all human skin specimens including uninjured skin as control. The number of MMP-$ 2^{+} $ macrophages was significantly increased in accordance with wound ages. In contrast to MMP-2, no MMP-$ 9^{+} $ signals were detected in uninjured and wound specimens aged less than 1 day. However, the number of MMP-$ 9^{+} $ macrophages profoundly appeared in groups II and III. Morphometrically, in all of wound samples aged 9–12 days, MMP-$ 2^{+} $ cell number was more than 20. On the contrary, most of the remaining samples had <20 positive cells. However, only one sample (a 7-day-old wound) showed 21 positive cells. Thus, with regard to practical applicability with forensic safety, MMP-$ 2^{+} $ macrophages of >20 would indicate a wound age of 7–12 days. Additionally, 10 out of 12 wound specimens aged 9–12 days showed the MMP-$ 2^{+} $ cell number of >25, implying that MMP-$ 2^{+} $ cell number of >25 would indicate the wound age of 9–12 days. On the contrary, all wound samples aged 3–14 days except for only one sample had MMP-$ 9^{+} $ cell number of >30, indicating that MMP-$ 9^{+} $ cell number of >30 would indicate the wound age of 3–14 days. Collectively, MMP-2 seemed to be more distinct marker, compared with MMP-9. |
| abstractGer |
Abstract We performed immunohistochemical study combined with morphometrical analyses in order to examine the expression of matrix metalloproteinase-2 (MMP-2) and MMP-9 using 55 human skin wounds of different ages: group I, 0–3 days (n = 16); II, 4–7 days (n = 11); III, 9–14 days (n = 16); and IV, 17–21 days (n = 12). Immunopositive reactions for MMP-2 were observed in all human skin specimens including uninjured skin as control. The number of MMP-$ 2^{+} $ macrophages was significantly increased in accordance with wound ages. In contrast to MMP-2, no MMP-$ 9^{+} $ signals were detected in uninjured and wound specimens aged less than 1 day. However, the number of MMP-$ 9^{+} $ macrophages profoundly appeared in groups II and III. Morphometrically, in all of wound samples aged 9–12 days, MMP-$ 2^{+} $ cell number was more than 20. On the contrary, most of the remaining samples had <20 positive cells. However, only one sample (a 7-day-old wound) showed 21 positive cells. Thus, with regard to practical applicability with forensic safety, MMP-$ 2^{+} $ macrophages of >20 would indicate a wound age of 7–12 days. Additionally, 10 out of 12 wound specimens aged 9–12 days showed the MMP-$ 2^{+} $ cell number of >25, implying that MMP-$ 2^{+} $ cell number of >25 would indicate the wound age of 9–12 days. On the contrary, all wound samples aged 3–14 days except for only one sample had MMP-$ 9^{+} $ cell number of >30, indicating that MMP-$ 9^{+} $ cell number of >30 would indicate the wound age of 3–14 days. Collectively, MMP-2 seemed to be more distinct marker, compared with MMP-9. |
| abstract_unstemmed |
Abstract We performed immunohistochemical study combined with morphometrical analyses in order to examine the expression of matrix metalloproteinase-2 (MMP-2) and MMP-9 using 55 human skin wounds of different ages: group I, 0–3 days (n = 16); II, 4–7 days (n = 11); III, 9–14 days (n = 16); and IV, 17–21 days (n = 12). Immunopositive reactions for MMP-2 were observed in all human skin specimens including uninjured skin as control. The number of MMP-$ 2^{+} $ macrophages was significantly increased in accordance with wound ages. In contrast to MMP-2, no MMP-$ 9^{+} $ signals were detected in uninjured and wound specimens aged less than 1 day. However, the number of MMP-$ 9^{+} $ macrophages profoundly appeared in groups II and III. Morphometrically, in all of wound samples aged 9–12 days, MMP-$ 2^{+} $ cell number was more than 20. On the contrary, most of the remaining samples had <20 positive cells. However, only one sample (a 7-day-old wound) showed 21 positive cells. Thus, with regard to practical applicability with forensic safety, MMP-$ 2^{+} $ macrophages of >20 would indicate a wound age of 7–12 days. Additionally, 10 out of 12 wound specimens aged 9–12 days showed the MMP-$ 2^{+} $ cell number of >25, implying that MMP-$ 2^{+} $ cell number of >25 would indicate the wound age of 9–12 days. On the contrary, all wound samples aged 3–14 days except for only one sample had MMP-$ 9^{+} $ cell number of >30, indicating that MMP-$ 9^{+} $ cell number of >30 would indicate the wound age of 3–14 days. Collectively, MMP-2 seemed to be more distinct marker, compared with MMP-9. |
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5 |
| title_short |
Immunohistochemical analysis on MMP-2 and MMP-9 for wound age determination |
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https://dx.doi.org/10.1007/s00414-015-1167-5 |
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| author2 |
Kuninaka, Yumi Nosaka, Mizuho Kimura, Akihiko Kawaguchi, Takashi Hama, Mizuki Sakamoto, Shoichi Shinozaki, Kouhei Eisenmenger, Wolfgang Kondo, Toshikazu |
| author2Str |
Kuninaka, Yumi Nosaka, Mizuho Kimura, Akihiko Kawaguchi, Takashi Hama, Mizuki Sakamoto, Shoichi Shinozaki, Kouhei Eisenmenger, Wolfgang Kondo, Toshikazu |
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| doi_str |
10.1007/s00414-015-1167-5 |
| up_date |
2024-07-03T15:22:30.055Z |
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| score |
7.402158 |