Immunohistochemical localization of ghrelin in rodent kidneys
Abstract Ghrelin is a novel peptide hormone, originally identified in the rat and human stomach that plays various important roles. In the present study, we report the intra-renal localization of ghrelin in laboratory rodents. Kidneys from 3 month-old mice, rats and hamsters of both sexes were analy...
Ausführliche Beschreibung
Autor*in: |
Yabuki, Akira [verfasserIn] Taharaguchi, Satoshi [verfasserIn] Ichii, Osamu [verfasserIn] Kojima, Masayasu [verfasserIn] Nishi, Yoshihiro [verfasserIn] Mifune, Hiroharu [verfasserIn] Kamimura, Ryozo [verfasserIn] Matsumoto, Mitsuharu [verfasserIn] Suzuki, Shusaku [verfasserIn] |
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Format: |
E-Artikel |
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Sprache: |
Englisch |
Erschienen: |
2006 |
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Schlagwörter: |
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Übergeordnetes Werk: |
Enthalten in: Histochemistry and cell biology - Berlin : Springer, 1958, 126(2006), 2 vom: 03. März, Seite 231-238 |
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Übergeordnetes Werk: |
volume:126 ; year:2006 ; number:2 ; day:03 ; month:03 ; pages:231-238 |
Links: |
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DOI / URN: |
10.1007/s00418-006-0165-6 |
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Katalog-ID: |
SPR005449308 |
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245 | 1 | 0 | |a Immunohistochemical localization of ghrelin in rodent kidneys |
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520 | |a Abstract Ghrelin is a novel peptide hormone, originally identified in the rat and human stomach that plays various important roles. In the present study, we report the intra-renal localization of ghrelin in laboratory rodents. Kidneys from 3 month-old mice, rats and hamsters of both sexes were analyzed by immunohistochemistry. Positive signals were clearly observed in the epithelium of the distal tubules, whereas other segments of the nephron or interstitial cells, including juxtaglomerular cells, showed negative reactions. Pre-embedding immunoelectron microscopy revealed positive signals exclusively on the basolateral membrane in the distal tubular cells and in the collecting ducts. In addition, prepro-ghrelin gene expression was assessed by RT-PCR, and the expected 329-bp prepro-ghrelin mRNA was clearly detected in the kidney. On Western blot analysis, although a specific band for ghrelin (3 kDa) was not detected in the kidney, the expected band for prepro-ghrelin (13 kDa) was clearly detected in both the stomach and the kidney. This paper clarified the intra-renal localization of ghrelin. | ||
650 | 4 | |a Basolateral membrane |7 (dpeaa)DE-He213 | |
650 | 4 | |a Distal tubule |7 (dpeaa)DE-He213 | |
650 | 4 | |a Ghrelin |7 (dpeaa)DE-He213 | |
650 | 4 | |a Kidney |7 (dpeaa)DE-He213 | |
650 | 4 | |a Prepro-ghrelin |7 (dpeaa)DE-He213 | |
700 | 1 | |a Taharaguchi, Satoshi |e verfasserin |4 aut | |
700 | 1 | |a Ichii, Osamu |e verfasserin |4 aut | |
700 | 1 | |a Kojima, Masayasu |e verfasserin |4 aut | |
700 | 1 | |a Nishi, Yoshihiro |e verfasserin |4 aut | |
700 | 1 | |a Mifune, Hiroharu |e verfasserin |4 aut | |
700 | 1 | |a Kamimura, Ryozo |e verfasserin |4 aut | |
700 | 1 | |a Matsumoto, Mitsuharu |e verfasserin |4 aut | |
700 | 1 | |a Suzuki, Shusaku |e verfasserin |4 aut | |
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44.34 42.00 |
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2006 |
allfields |
10.1007/s00418-006-0165-6 doi (DE-627)SPR005449308 (SPR)s00418-006-0165-6-e DE-627 ger DE-627 rakwb eng 610 ASE 44.34 bkl 42.00 bkl Yabuki, Akira verfasserin aut Immunohistochemical localization of ghrelin in rodent kidneys 2006 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Ghrelin is a novel peptide hormone, originally identified in the rat and human stomach that plays various important roles. In the present study, we report the intra-renal localization of ghrelin in laboratory rodents. Kidneys from 3 month-old mice, rats and hamsters of both sexes were analyzed by immunohistochemistry. Positive signals were clearly observed in the epithelium of the distal tubules, whereas other segments of the nephron or interstitial cells, including juxtaglomerular cells, showed negative reactions. Pre-embedding immunoelectron microscopy revealed positive signals exclusively on the basolateral membrane in the distal tubular cells and in the collecting ducts. In addition, prepro-ghrelin gene expression was assessed by RT-PCR, and the expected 329-bp prepro-ghrelin mRNA was clearly detected in the kidney. On Western blot analysis, although a specific band for ghrelin (3 kDa) was not detected in the kidney, the expected band for prepro-ghrelin (13 kDa) was clearly detected in both the stomach and the kidney. This paper clarified the intra-renal localization of ghrelin. Basolateral membrane (dpeaa)DE-He213 Distal tubule (dpeaa)DE-He213 Ghrelin (dpeaa)DE-He213 Kidney (dpeaa)DE-He213 Prepro-ghrelin (dpeaa)DE-He213 Taharaguchi, Satoshi verfasserin aut Ichii, Osamu verfasserin aut Kojima, Masayasu verfasserin aut Nishi, Yoshihiro verfasserin aut Mifune, Hiroharu verfasserin aut Kamimura, Ryozo verfasserin aut Matsumoto, Mitsuharu verfasserin aut Suzuki, Shusaku verfasserin aut Enthalten in Histochemistry and cell biology Berlin : Springer, 1958 126(2006), 2 vom: 03. März, Seite 231-238 (DE-627)235503568 (DE-600)1398345-3 1432-119X nnns volume:126 year:2006 number:2 day:03 month:03 pages:231-238 https://dx.doi.org/10.1007/s00418-006-0165-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.34 ASE 42.00 ASE AR 126 2006 2 03 03 231-238 |
spelling |
10.1007/s00418-006-0165-6 doi (DE-627)SPR005449308 (SPR)s00418-006-0165-6-e DE-627 ger DE-627 rakwb eng 610 ASE 44.34 bkl 42.00 bkl Yabuki, Akira verfasserin aut Immunohistochemical localization of ghrelin in rodent kidneys 2006 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Ghrelin is a novel peptide hormone, originally identified in the rat and human stomach that plays various important roles. In the present study, we report the intra-renal localization of ghrelin in laboratory rodents. Kidneys from 3 month-old mice, rats and hamsters of both sexes were analyzed by immunohistochemistry. Positive signals were clearly observed in the epithelium of the distal tubules, whereas other segments of the nephron or interstitial cells, including juxtaglomerular cells, showed negative reactions. Pre-embedding immunoelectron microscopy revealed positive signals exclusively on the basolateral membrane in the distal tubular cells and in the collecting ducts. In addition, prepro-ghrelin gene expression was assessed by RT-PCR, and the expected 329-bp prepro-ghrelin mRNA was clearly detected in the kidney. On Western blot analysis, although a specific band for ghrelin (3 kDa) was not detected in the kidney, the expected band for prepro-ghrelin (13 kDa) was clearly detected in both the stomach and the kidney. This paper clarified the intra-renal localization of ghrelin. Basolateral membrane (dpeaa)DE-He213 Distal tubule (dpeaa)DE-He213 Ghrelin (dpeaa)DE-He213 Kidney (dpeaa)DE-He213 Prepro-ghrelin (dpeaa)DE-He213 Taharaguchi, Satoshi verfasserin aut Ichii, Osamu verfasserin aut Kojima, Masayasu verfasserin aut Nishi, Yoshihiro verfasserin aut Mifune, Hiroharu verfasserin aut Kamimura, Ryozo verfasserin aut Matsumoto, Mitsuharu verfasserin aut Suzuki, Shusaku verfasserin aut Enthalten in Histochemistry and cell biology Berlin : Springer, 1958 126(2006), 2 vom: 03. März, Seite 231-238 (DE-627)235503568 (DE-600)1398345-3 1432-119X nnns volume:126 year:2006 number:2 day:03 month:03 pages:231-238 https://dx.doi.org/10.1007/s00418-006-0165-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.34 ASE 42.00 ASE AR 126 2006 2 03 03 231-238 |
allfields_unstemmed |
10.1007/s00418-006-0165-6 doi (DE-627)SPR005449308 (SPR)s00418-006-0165-6-e DE-627 ger DE-627 rakwb eng 610 ASE 44.34 bkl 42.00 bkl Yabuki, Akira verfasserin aut Immunohistochemical localization of ghrelin in rodent kidneys 2006 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Ghrelin is a novel peptide hormone, originally identified in the rat and human stomach that plays various important roles. In the present study, we report the intra-renal localization of ghrelin in laboratory rodents. Kidneys from 3 month-old mice, rats and hamsters of both sexes were analyzed by immunohistochemistry. Positive signals were clearly observed in the epithelium of the distal tubules, whereas other segments of the nephron or interstitial cells, including juxtaglomerular cells, showed negative reactions. Pre-embedding immunoelectron microscopy revealed positive signals exclusively on the basolateral membrane in the distal tubular cells and in the collecting ducts. In addition, prepro-ghrelin gene expression was assessed by RT-PCR, and the expected 329-bp prepro-ghrelin mRNA was clearly detected in the kidney. On Western blot analysis, although a specific band for ghrelin (3 kDa) was not detected in the kidney, the expected band for prepro-ghrelin (13 kDa) was clearly detected in both the stomach and the kidney. This paper clarified the intra-renal localization of ghrelin. Basolateral membrane (dpeaa)DE-He213 Distal tubule (dpeaa)DE-He213 Ghrelin (dpeaa)DE-He213 Kidney (dpeaa)DE-He213 Prepro-ghrelin (dpeaa)DE-He213 Taharaguchi, Satoshi verfasserin aut Ichii, Osamu verfasserin aut Kojima, Masayasu verfasserin aut Nishi, Yoshihiro verfasserin aut Mifune, Hiroharu verfasserin aut Kamimura, Ryozo verfasserin aut Matsumoto, Mitsuharu verfasserin aut Suzuki, Shusaku verfasserin aut Enthalten in Histochemistry and cell biology Berlin : Springer, 1958 126(2006), 2 vom: 03. März, Seite 231-238 (DE-627)235503568 (DE-600)1398345-3 1432-119X nnns volume:126 year:2006 number:2 day:03 month:03 pages:231-238 https://dx.doi.org/10.1007/s00418-006-0165-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.34 ASE 42.00 ASE AR 126 2006 2 03 03 231-238 |
allfieldsGer |
10.1007/s00418-006-0165-6 doi (DE-627)SPR005449308 (SPR)s00418-006-0165-6-e DE-627 ger DE-627 rakwb eng 610 ASE 44.34 bkl 42.00 bkl Yabuki, Akira verfasserin aut Immunohistochemical localization of ghrelin in rodent kidneys 2006 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Ghrelin is a novel peptide hormone, originally identified in the rat and human stomach that plays various important roles. In the present study, we report the intra-renal localization of ghrelin in laboratory rodents. Kidneys from 3 month-old mice, rats and hamsters of both sexes were analyzed by immunohistochemistry. Positive signals were clearly observed in the epithelium of the distal tubules, whereas other segments of the nephron or interstitial cells, including juxtaglomerular cells, showed negative reactions. Pre-embedding immunoelectron microscopy revealed positive signals exclusively on the basolateral membrane in the distal tubular cells and in the collecting ducts. In addition, prepro-ghrelin gene expression was assessed by RT-PCR, and the expected 329-bp prepro-ghrelin mRNA was clearly detected in the kidney. On Western blot analysis, although a specific band for ghrelin (3 kDa) was not detected in the kidney, the expected band for prepro-ghrelin (13 kDa) was clearly detected in both the stomach and the kidney. This paper clarified the intra-renal localization of ghrelin. Basolateral membrane (dpeaa)DE-He213 Distal tubule (dpeaa)DE-He213 Ghrelin (dpeaa)DE-He213 Kidney (dpeaa)DE-He213 Prepro-ghrelin (dpeaa)DE-He213 Taharaguchi, Satoshi verfasserin aut Ichii, Osamu verfasserin aut Kojima, Masayasu verfasserin aut Nishi, Yoshihiro verfasserin aut Mifune, Hiroharu verfasserin aut Kamimura, Ryozo verfasserin aut Matsumoto, Mitsuharu verfasserin aut Suzuki, Shusaku verfasserin aut Enthalten in Histochemistry and cell biology Berlin : Springer, 1958 126(2006), 2 vom: 03. März, Seite 231-238 (DE-627)235503568 (DE-600)1398345-3 1432-119X nnns volume:126 year:2006 number:2 day:03 month:03 pages:231-238 https://dx.doi.org/10.1007/s00418-006-0165-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.34 ASE 42.00 ASE AR 126 2006 2 03 03 231-238 |
allfieldsSound |
10.1007/s00418-006-0165-6 doi (DE-627)SPR005449308 (SPR)s00418-006-0165-6-e DE-627 ger DE-627 rakwb eng 610 ASE 44.34 bkl 42.00 bkl Yabuki, Akira verfasserin aut Immunohistochemical localization of ghrelin in rodent kidneys 2006 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Ghrelin is a novel peptide hormone, originally identified in the rat and human stomach that plays various important roles. In the present study, we report the intra-renal localization of ghrelin in laboratory rodents. Kidneys from 3 month-old mice, rats and hamsters of both sexes were analyzed by immunohistochemistry. Positive signals were clearly observed in the epithelium of the distal tubules, whereas other segments of the nephron or interstitial cells, including juxtaglomerular cells, showed negative reactions. Pre-embedding immunoelectron microscopy revealed positive signals exclusively on the basolateral membrane in the distal tubular cells and in the collecting ducts. In addition, prepro-ghrelin gene expression was assessed by RT-PCR, and the expected 329-bp prepro-ghrelin mRNA was clearly detected in the kidney. On Western blot analysis, although a specific band for ghrelin (3 kDa) was not detected in the kidney, the expected band for prepro-ghrelin (13 kDa) was clearly detected in both the stomach and the kidney. This paper clarified the intra-renal localization of ghrelin. Basolateral membrane (dpeaa)DE-He213 Distal tubule (dpeaa)DE-He213 Ghrelin (dpeaa)DE-He213 Kidney (dpeaa)DE-He213 Prepro-ghrelin (dpeaa)DE-He213 Taharaguchi, Satoshi verfasserin aut Ichii, Osamu verfasserin aut Kojima, Masayasu verfasserin aut Nishi, Yoshihiro verfasserin aut Mifune, Hiroharu verfasserin aut Kamimura, Ryozo verfasserin aut Matsumoto, Mitsuharu verfasserin aut Suzuki, Shusaku verfasserin aut Enthalten in Histochemistry and cell biology Berlin : Springer, 1958 126(2006), 2 vom: 03. März, Seite 231-238 (DE-627)235503568 (DE-600)1398345-3 1432-119X nnns volume:126 year:2006 number:2 day:03 month:03 pages:231-238 https://dx.doi.org/10.1007/s00418-006-0165-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.34 ASE 42.00 ASE AR 126 2006 2 03 03 231-238 |
language |
English |
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Enthalten in Histochemistry and cell biology 126(2006), 2 vom: 03. März, Seite 231-238 volume:126 year:2006 number:2 day:03 month:03 pages:231-238 |
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Enthalten in Histochemistry and cell biology 126(2006), 2 vom: 03. März, Seite 231-238 volume:126 year:2006 number:2 day:03 month:03 pages:231-238 |
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Basolateral membrane Distal tubule Ghrelin Kidney Prepro-ghrelin |
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610 |
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Histochemistry and cell biology |
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Yabuki, Akira @@aut@@ Taharaguchi, Satoshi @@aut@@ Ichii, Osamu @@aut@@ Kojima, Masayasu @@aut@@ Nishi, Yoshihiro @@aut@@ Mifune, Hiroharu @@aut@@ Kamimura, Ryozo @@aut@@ Matsumoto, Mitsuharu @@aut@@ Suzuki, Shusaku @@aut@@ |
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2006-03-03T00:00:00Z |
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3610 |
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|
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Yabuki, Akira |
spellingShingle |
Yabuki, Akira ddc 610 bkl 44.34 bkl 42.00 misc Basolateral membrane misc Distal tubule misc Ghrelin misc Kidney misc Prepro-ghrelin Immunohistochemical localization of ghrelin in rodent kidneys |
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610 ASE 44.34 bkl 42.00 bkl Immunohistochemical localization of ghrelin in rodent kidneys Basolateral membrane (dpeaa)DE-He213 Distal tubule (dpeaa)DE-He213 Ghrelin (dpeaa)DE-He213 Kidney (dpeaa)DE-He213 Prepro-ghrelin (dpeaa)DE-He213 |
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ddc 610 bkl 44.34 bkl 42.00 misc Basolateral membrane misc Distal tubule misc Ghrelin misc Kidney misc Prepro-ghrelin |
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ddc 610 bkl 44.34 bkl 42.00 misc Basolateral membrane misc Distal tubule misc Ghrelin misc Kidney misc Prepro-ghrelin |
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ddc 610 bkl 44.34 bkl 42.00 misc Basolateral membrane misc Distal tubule misc Ghrelin misc Kidney misc Prepro-ghrelin |
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Immunohistochemical localization of ghrelin in rodent kidneys |
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Immunohistochemical localization of ghrelin in rodent kidneys |
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Yabuki, Akira |
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Histochemistry and cell biology |
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Yabuki, Akira Taharaguchi, Satoshi Ichii, Osamu Kojima, Masayasu Nishi, Yoshihiro Mifune, Hiroharu Kamimura, Ryozo Matsumoto, Mitsuharu Suzuki, Shusaku |
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immunohistochemical localization of ghrelin in rodent kidneys |
title_auth |
Immunohistochemical localization of ghrelin in rodent kidneys |
abstract |
Abstract Ghrelin is a novel peptide hormone, originally identified in the rat and human stomach that plays various important roles. In the present study, we report the intra-renal localization of ghrelin in laboratory rodents. Kidneys from 3 month-old mice, rats and hamsters of both sexes were analyzed by immunohistochemistry. Positive signals were clearly observed in the epithelium of the distal tubules, whereas other segments of the nephron or interstitial cells, including juxtaglomerular cells, showed negative reactions. Pre-embedding immunoelectron microscopy revealed positive signals exclusively on the basolateral membrane in the distal tubular cells and in the collecting ducts. In addition, prepro-ghrelin gene expression was assessed by RT-PCR, and the expected 329-bp prepro-ghrelin mRNA was clearly detected in the kidney. On Western blot analysis, although a specific band for ghrelin (3 kDa) was not detected in the kidney, the expected band for prepro-ghrelin (13 kDa) was clearly detected in both the stomach and the kidney. This paper clarified the intra-renal localization of ghrelin. |
abstractGer |
Abstract Ghrelin is a novel peptide hormone, originally identified in the rat and human stomach that plays various important roles. In the present study, we report the intra-renal localization of ghrelin in laboratory rodents. Kidneys from 3 month-old mice, rats and hamsters of both sexes were analyzed by immunohistochemistry. Positive signals were clearly observed in the epithelium of the distal tubules, whereas other segments of the nephron or interstitial cells, including juxtaglomerular cells, showed negative reactions. Pre-embedding immunoelectron microscopy revealed positive signals exclusively on the basolateral membrane in the distal tubular cells and in the collecting ducts. In addition, prepro-ghrelin gene expression was assessed by RT-PCR, and the expected 329-bp prepro-ghrelin mRNA was clearly detected in the kidney. On Western blot analysis, although a specific band for ghrelin (3 kDa) was not detected in the kidney, the expected band for prepro-ghrelin (13 kDa) was clearly detected in both the stomach and the kidney. This paper clarified the intra-renal localization of ghrelin. |
abstract_unstemmed |
Abstract Ghrelin is a novel peptide hormone, originally identified in the rat and human stomach that plays various important roles. In the present study, we report the intra-renal localization of ghrelin in laboratory rodents. Kidneys from 3 month-old mice, rats and hamsters of both sexes were analyzed by immunohistochemistry. Positive signals were clearly observed in the epithelium of the distal tubules, whereas other segments of the nephron or interstitial cells, including juxtaglomerular cells, showed negative reactions. Pre-embedding immunoelectron microscopy revealed positive signals exclusively on the basolateral membrane in the distal tubular cells and in the collecting ducts. In addition, prepro-ghrelin gene expression was assessed by RT-PCR, and the expected 329-bp prepro-ghrelin mRNA was clearly detected in the kidney. On Western blot analysis, although a specific band for ghrelin (3 kDa) was not detected in the kidney, the expected band for prepro-ghrelin (13 kDa) was clearly detected in both the stomach and the kidney. This paper clarified the intra-renal localization of ghrelin. |
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Immunohistochemical localization of ghrelin in rodent kidneys |
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https://dx.doi.org/10.1007/s00418-006-0165-6 |
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Taharaguchi, Satoshi Ichii, Osamu Kojima, Masayasu Nishi, Yoshihiro Mifune, Hiroharu Kamimura, Ryozo Matsumoto, Mitsuharu Suzuki, Shusaku |
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Taharaguchi, Satoshi Ichii, Osamu Kojima, Masayasu Nishi, Yoshihiro Mifune, Hiroharu Kamimura, Ryozo Matsumoto, Mitsuharu Suzuki, Shusaku |
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|
score |
7.398752 |