Expression and function of the epithelial sodium channel δ-subunit in human respiratory epithelial cells in vitro
Abstract Using human airway epithelial cell lines (i.e. NCI-H441 and Calu-3) as well as human alveolar epithelial type I-like (ATI) cells in primary culture, we studied the contribution of the epithelial sodium channel δ-subunit (δ-ENaC) to transepithelial sodium transport in human lung in vitro. En...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Schwagerus, Elena [verfasserIn] Sladek, Svenja [verfasserIn] Buckley, Stephen T. [verfasserIn] Armas-Capote, Natalia [verfasserIn] de la Rosa, Diego Alvarez [verfasserIn] Harvey, Brian J. [verfasserIn] Fischer, Horst [verfasserIn] Illek, Beate [verfasserIn] Huwer, Hanno [verfasserIn] Schneider-Daum, Nicole [verfasserIn] Lehr, Claus-Michael [verfasserIn] Ehrhardt, Carsten [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2015 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Pflügers Archiv - Berlin : Springer, 1868, 467(2015), 11 vom: 13. Feb., Seite 2257-2273 |
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| Übergeordnetes Werk: |
volume:467 ; year:2015 ; number:11 ; day:13 ; month:02 ; pages:2257-2273 |
| Links: |
|---|
| DOI / URN: |
10.1007/s00424-015-1693-5 |
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| Katalog-ID: |
SPR005621712 |
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| 520 | |a Abstract Using human airway epithelial cell lines (i.e. NCI-H441 and Calu-3) as well as human alveolar epithelial type I-like (ATI) cells in primary culture, we studied the contribution of the epithelial sodium channel δ-subunit (δ-ENaC) to transepithelial sodium transport in human lung in vitro. Endogenous δ-ENaC protein was present in all three cell types tested; however, protein abundance was low, and no expression was detected in the apical cell membrane of these cells. Similarly, known modulators of δ-ENaC activity, such as capsazepine and icilin (activators) and Evans blue (inhibitor), did not show effects on short-circuit current (ISC), suggesting that δ-ENaC is not involved in the modulation of transcellular sodium absorption in NCI-H441 cell monolayers. Over-expression of δ-ENaC in NCI-H441 cells resulted in detectable protein expression in the apical cell membrane, as well as capsazepine and icilin-stimulated increases in ISC that were effectively blocked by Evans blue and that were consistent with δ-ENaC activation and inhibition, respectively. Consequently, these observations suggest that δ-ENaC expression is low in NCI-H441, Calu-3, and ATI cells and does not contribute to transepithelial sodium absorption. | ||
| 650 | 4 | |a Airway epithelium |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Alveolar epithelium |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a ENaC |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Ussing chamber |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Sodium absorption |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Sladek, Svenja |e verfasserin |4 aut | |
| 700 | 1 | |a Buckley, Stephen T. |e verfasserin |4 aut | |
| 700 | 1 | |a Armas-Capote, Natalia |e verfasserin |4 aut | |
| 700 | 1 | |a de la Rosa, Diego Alvarez |e verfasserin |4 aut | |
| 700 | 1 | |a Harvey, Brian J. |e verfasserin |4 aut | |
| 700 | 1 | |a Fischer, Horst |e verfasserin |4 aut | |
| 700 | 1 | |a Illek, Beate |e verfasserin |4 aut | |
| 700 | 1 | |a Huwer, Hanno |e verfasserin |4 aut | |
| 700 | 1 | |a Schneider-Daum, Nicole |e verfasserin |4 aut | |
| 700 | 1 | |a Lehr, Claus-Michael |e verfasserin |4 aut | |
| 700 | 1 | |a Ehrhardt, Carsten |e verfasserin |4 aut | |
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10.1007/s00424-015-1693-5 doi (DE-627)SPR005621712 (SPR)s00424-015-1693-5-e DE-627 ger DE-627 rakwb eng 610 ASE 610 590 ASE 42.63 bkl 44.37 bkl Schwagerus, Elena verfasserin aut Expression and function of the epithelial sodium channel δ-subunit in human respiratory epithelial cells in vitro 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Using human airway epithelial cell lines (i.e. NCI-H441 and Calu-3) as well as human alveolar epithelial type I-like (ATI) cells in primary culture, we studied the contribution of the epithelial sodium channel δ-subunit (δ-ENaC) to transepithelial sodium transport in human lung in vitro. Endogenous δ-ENaC protein was present in all three cell types tested; however, protein abundance was low, and no expression was detected in the apical cell membrane of these cells. Similarly, known modulators of δ-ENaC activity, such as capsazepine and icilin (activators) and Evans blue (inhibitor), did not show effects on short-circuit current (ISC), suggesting that δ-ENaC is not involved in the modulation of transcellular sodium absorption in NCI-H441 cell monolayers. Over-expression of δ-ENaC in NCI-H441 cells resulted in detectable protein expression in the apical cell membrane, as well as capsazepine and icilin-stimulated increases in ISC that were effectively blocked by Evans blue and that were consistent with δ-ENaC activation and inhibition, respectively. Consequently, these observations suggest that δ-ENaC expression is low in NCI-H441, Calu-3, and ATI cells and does not contribute to transepithelial sodium absorption. Airway epithelium (dpeaa)DE-He213 Alveolar epithelium (dpeaa)DE-He213 ENaC (dpeaa)DE-He213 Ussing chamber (dpeaa)DE-He213 Sodium absorption (dpeaa)DE-He213 Sladek, Svenja verfasserin aut Buckley, Stephen T. verfasserin aut Armas-Capote, Natalia verfasserin aut de la Rosa, Diego Alvarez verfasserin aut Harvey, Brian J. verfasserin aut Fischer, Horst verfasserin aut Illek, Beate verfasserin aut Huwer, Hanno verfasserin aut Schneider-Daum, Nicole verfasserin aut Lehr, Claus-Michael verfasserin aut Ehrhardt, Carsten verfasserin aut Enthalten in Pflügers Archiv Berlin : Springer, 1868 467(2015), 11 vom: 13. Feb., Seite 2257-2273 (DE-627)25463897X (DE-600)1463014-X 1432-2013 nnns volume:467 year:2015 number:11 day:13 month:02 pages:2257-2273 https://dx.doi.org/10.1007/s00424-015-1693-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.63 ASE 44.37 ASE AR 467 2015 11 13 02 2257-2273 |
| spelling |
10.1007/s00424-015-1693-5 doi (DE-627)SPR005621712 (SPR)s00424-015-1693-5-e DE-627 ger DE-627 rakwb eng 610 ASE 610 590 ASE 42.63 bkl 44.37 bkl Schwagerus, Elena verfasserin aut Expression and function of the epithelial sodium channel δ-subunit in human respiratory epithelial cells in vitro 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Using human airway epithelial cell lines (i.e. NCI-H441 and Calu-3) as well as human alveolar epithelial type I-like (ATI) cells in primary culture, we studied the contribution of the epithelial sodium channel δ-subunit (δ-ENaC) to transepithelial sodium transport in human lung in vitro. Endogenous δ-ENaC protein was present in all three cell types tested; however, protein abundance was low, and no expression was detected in the apical cell membrane of these cells. Similarly, known modulators of δ-ENaC activity, such as capsazepine and icilin (activators) and Evans blue (inhibitor), did not show effects on short-circuit current (ISC), suggesting that δ-ENaC is not involved in the modulation of transcellular sodium absorption in NCI-H441 cell monolayers. Over-expression of δ-ENaC in NCI-H441 cells resulted in detectable protein expression in the apical cell membrane, as well as capsazepine and icilin-stimulated increases in ISC that were effectively blocked by Evans blue and that were consistent with δ-ENaC activation and inhibition, respectively. Consequently, these observations suggest that δ-ENaC expression is low in NCI-H441, Calu-3, and ATI cells and does not contribute to transepithelial sodium absorption. Airway epithelium (dpeaa)DE-He213 Alveolar epithelium (dpeaa)DE-He213 ENaC (dpeaa)DE-He213 Ussing chamber (dpeaa)DE-He213 Sodium absorption (dpeaa)DE-He213 Sladek, Svenja verfasserin aut Buckley, Stephen T. verfasserin aut Armas-Capote, Natalia verfasserin aut de la Rosa, Diego Alvarez verfasserin aut Harvey, Brian J. verfasserin aut Fischer, Horst verfasserin aut Illek, Beate verfasserin aut Huwer, Hanno verfasserin aut Schneider-Daum, Nicole verfasserin aut Lehr, Claus-Michael verfasserin aut Ehrhardt, Carsten verfasserin aut Enthalten in Pflügers Archiv Berlin : Springer, 1868 467(2015), 11 vom: 13. Feb., Seite 2257-2273 (DE-627)25463897X (DE-600)1463014-X 1432-2013 nnns volume:467 year:2015 number:11 day:13 month:02 pages:2257-2273 https://dx.doi.org/10.1007/s00424-015-1693-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.63 ASE 44.37 ASE AR 467 2015 11 13 02 2257-2273 |
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10.1007/s00424-015-1693-5 doi (DE-627)SPR005621712 (SPR)s00424-015-1693-5-e DE-627 ger DE-627 rakwb eng 610 ASE 610 590 ASE 42.63 bkl 44.37 bkl Schwagerus, Elena verfasserin aut Expression and function of the epithelial sodium channel δ-subunit in human respiratory epithelial cells in vitro 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Using human airway epithelial cell lines (i.e. NCI-H441 and Calu-3) as well as human alveolar epithelial type I-like (ATI) cells in primary culture, we studied the contribution of the epithelial sodium channel δ-subunit (δ-ENaC) to transepithelial sodium transport in human lung in vitro. Endogenous δ-ENaC protein was present in all three cell types tested; however, protein abundance was low, and no expression was detected in the apical cell membrane of these cells. Similarly, known modulators of δ-ENaC activity, such as capsazepine and icilin (activators) and Evans blue (inhibitor), did not show effects on short-circuit current (ISC), suggesting that δ-ENaC is not involved in the modulation of transcellular sodium absorption in NCI-H441 cell monolayers. Over-expression of δ-ENaC in NCI-H441 cells resulted in detectable protein expression in the apical cell membrane, as well as capsazepine and icilin-stimulated increases in ISC that were effectively blocked by Evans blue and that were consistent with δ-ENaC activation and inhibition, respectively. Consequently, these observations suggest that δ-ENaC expression is low in NCI-H441, Calu-3, and ATI cells and does not contribute to transepithelial sodium absorption. Airway epithelium (dpeaa)DE-He213 Alveolar epithelium (dpeaa)DE-He213 ENaC (dpeaa)DE-He213 Ussing chamber (dpeaa)DE-He213 Sodium absorption (dpeaa)DE-He213 Sladek, Svenja verfasserin aut Buckley, Stephen T. verfasserin aut Armas-Capote, Natalia verfasserin aut de la Rosa, Diego Alvarez verfasserin aut Harvey, Brian J. verfasserin aut Fischer, Horst verfasserin aut Illek, Beate verfasserin aut Huwer, Hanno verfasserin aut Schneider-Daum, Nicole verfasserin aut Lehr, Claus-Michael verfasserin aut Ehrhardt, Carsten verfasserin aut Enthalten in Pflügers Archiv Berlin : Springer, 1868 467(2015), 11 vom: 13. Feb., Seite 2257-2273 (DE-627)25463897X (DE-600)1463014-X 1432-2013 nnns volume:467 year:2015 number:11 day:13 month:02 pages:2257-2273 https://dx.doi.org/10.1007/s00424-015-1693-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.63 ASE 44.37 ASE AR 467 2015 11 13 02 2257-2273 |
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10.1007/s00424-015-1693-5 doi (DE-627)SPR005621712 (SPR)s00424-015-1693-5-e DE-627 ger DE-627 rakwb eng 610 ASE 610 590 ASE 42.63 bkl 44.37 bkl Schwagerus, Elena verfasserin aut Expression and function of the epithelial sodium channel δ-subunit in human respiratory epithelial cells in vitro 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Using human airway epithelial cell lines (i.e. NCI-H441 and Calu-3) as well as human alveolar epithelial type I-like (ATI) cells in primary culture, we studied the contribution of the epithelial sodium channel δ-subunit (δ-ENaC) to transepithelial sodium transport in human lung in vitro. Endogenous δ-ENaC protein was present in all three cell types tested; however, protein abundance was low, and no expression was detected in the apical cell membrane of these cells. Similarly, known modulators of δ-ENaC activity, such as capsazepine and icilin (activators) and Evans blue (inhibitor), did not show effects on short-circuit current (ISC), suggesting that δ-ENaC is not involved in the modulation of transcellular sodium absorption in NCI-H441 cell monolayers. Over-expression of δ-ENaC in NCI-H441 cells resulted in detectable protein expression in the apical cell membrane, as well as capsazepine and icilin-stimulated increases in ISC that were effectively blocked by Evans blue and that were consistent with δ-ENaC activation and inhibition, respectively. Consequently, these observations suggest that δ-ENaC expression is low in NCI-H441, Calu-3, and ATI cells and does not contribute to transepithelial sodium absorption. Airway epithelium (dpeaa)DE-He213 Alveolar epithelium (dpeaa)DE-He213 ENaC (dpeaa)DE-He213 Ussing chamber (dpeaa)DE-He213 Sodium absorption (dpeaa)DE-He213 Sladek, Svenja verfasserin aut Buckley, Stephen T. verfasserin aut Armas-Capote, Natalia verfasserin aut de la Rosa, Diego Alvarez verfasserin aut Harvey, Brian J. verfasserin aut Fischer, Horst verfasserin aut Illek, Beate verfasserin aut Huwer, Hanno verfasserin aut Schneider-Daum, Nicole verfasserin aut Lehr, Claus-Michael verfasserin aut Ehrhardt, Carsten verfasserin aut Enthalten in Pflügers Archiv Berlin : Springer, 1868 467(2015), 11 vom: 13. Feb., Seite 2257-2273 (DE-627)25463897X (DE-600)1463014-X 1432-2013 nnns volume:467 year:2015 number:11 day:13 month:02 pages:2257-2273 https://dx.doi.org/10.1007/s00424-015-1693-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.63 ASE 44.37 ASE AR 467 2015 11 13 02 2257-2273 |
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10.1007/s00424-015-1693-5 doi (DE-627)SPR005621712 (SPR)s00424-015-1693-5-e DE-627 ger DE-627 rakwb eng 610 ASE 610 590 ASE 42.63 bkl 44.37 bkl Schwagerus, Elena verfasserin aut Expression and function of the epithelial sodium channel δ-subunit in human respiratory epithelial cells in vitro 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Using human airway epithelial cell lines (i.e. NCI-H441 and Calu-3) as well as human alveolar epithelial type I-like (ATI) cells in primary culture, we studied the contribution of the epithelial sodium channel δ-subunit (δ-ENaC) to transepithelial sodium transport in human lung in vitro. Endogenous δ-ENaC protein was present in all three cell types tested; however, protein abundance was low, and no expression was detected in the apical cell membrane of these cells. Similarly, known modulators of δ-ENaC activity, such as capsazepine and icilin (activators) and Evans blue (inhibitor), did not show effects on short-circuit current (ISC), suggesting that δ-ENaC is not involved in the modulation of transcellular sodium absorption in NCI-H441 cell monolayers. Over-expression of δ-ENaC in NCI-H441 cells resulted in detectable protein expression in the apical cell membrane, as well as capsazepine and icilin-stimulated increases in ISC that were effectively blocked by Evans blue and that were consistent with δ-ENaC activation and inhibition, respectively. Consequently, these observations suggest that δ-ENaC expression is low in NCI-H441, Calu-3, and ATI cells and does not contribute to transepithelial sodium absorption. Airway epithelium (dpeaa)DE-He213 Alveolar epithelium (dpeaa)DE-He213 ENaC (dpeaa)DE-He213 Ussing chamber (dpeaa)DE-He213 Sodium absorption (dpeaa)DE-He213 Sladek, Svenja verfasserin aut Buckley, Stephen T. verfasserin aut Armas-Capote, Natalia verfasserin aut de la Rosa, Diego Alvarez verfasserin aut Harvey, Brian J. verfasserin aut Fischer, Horst verfasserin aut Illek, Beate verfasserin aut Huwer, Hanno verfasserin aut Schneider-Daum, Nicole verfasserin aut Lehr, Claus-Michael verfasserin aut Ehrhardt, Carsten verfasserin aut Enthalten in Pflügers Archiv Berlin : Springer, 1868 467(2015), 11 vom: 13. Feb., Seite 2257-2273 (DE-627)25463897X (DE-600)1463014-X 1432-2013 nnns volume:467 year:2015 number:11 day:13 month:02 pages:2257-2273 https://dx.doi.org/10.1007/s00424-015-1693-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.63 ASE 44.37 ASE AR 467 2015 11 13 02 2257-2273 |
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Enthalten in Pflügers Archiv 467(2015), 11 vom: 13. Feb., Seite 2257-2273 volume:467 year:2015 number:11 day:13 month:02 pages:2257-2273 |
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Airway epithelium Alveolar epithelium ENaC Ussing chamber Sodium absorption |
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Schwagerus, Elena @@aut@@ Sladek, Svenja @@aut@@ Buckley, Stephen T. @@aut@@ Armas-Capote, Natalia @@aut@@ de la Rosa, Diego Alvarez @@aut@@ Harvey, Brian J. @@aut@@ Fischer, Horst @@aut@@ Illek, Beate @@aut@@ Huwer, Hanno @@aut@@ Schneider-Daum, Nicole @@aut@@ Lehr, Claus-Michael @@aut@@ Ehrhardt, Carsten @@aut@@ |
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Endogenous δ-ENaC protein was present in all three cell types tested; however, protein abundance was low, and no expression was detected in the apical cell membrane of these cells. Similarly, known modulators of δ-ENaC activity, such as capsazepine and icilin (activators) and Evans blue (inhibitor), did not show effects on short-circuit current (ISC), suggesting that δ-ENaC is not involved in the modulation of transcellular sodium absorption in NCI-H441 cell monolayers. Over-expression of δ-ENaC in NCI-H441 cells resulted in detectable protein expression in the apical cell membrane, as well as capsazepine and icilin-stimulated increases in ISC that were effectively blocked by Evans blue and that were consistent with δ-ENaC activation and inhibition, respectively. 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|
| author |
Schwagerus, Elena |
| spellingShingle |
Schwagerus, Elena ddc 610 bkl 42.63 bkl 44.37 misc Airway epithelium misc Alveolar epithelium misc ENaC misc Ussing chamber misc Sodium absorption Expression and function of the epithelial sodium channel δ-subunit in human respiratory epithelial cells in vitro |
| authorStr |
Schwagerus, Elena |
| ppnlink_with_tag_str_mv |
@@773@@(DE-627)25463897X |
| format |
electronic Article |
| dewey-ones |
610 - Medicine & health 590 - Animals (Zoology) |
| delete_txt_mv |
keep |
| author_role |
aut aut aut aut aut aut aut aut aut aut aut aut |
| collection |
springer |
| remote_str |
true |
| illustrated |
Not Illustrated |
| issn |
1432-2013 |
| topic_title |
610 ASE 610 590 ASE 42.63 bkl 44.37 bkl Expression and function of the epithelial sodium channel δ-subunit in human respiratory epithelial cells in vitro Airway epithelium (dpeaa)DE-He213 Alveolar epithelium (dpeaa)DE-He213 ENaC (dpeaa)DE-He213 Ussing chamber (dpeaa)DE-He213 Sodium absorption (dpeaa)DE-He213 |
| topic |
ddc 610 bkl 42.63 bkl 44.37 misc Airway epithelium misc Alveolar epithelium misc ENaC misc Ussing chamber misc Sodium absorption |
| topic_unstemmed |
ddc 610 bkl 42.63 bkl 44.37 misc Airway epithelium misc Alveolar epithelium misc ENaC misc Ussing chamber misc Sodium absorption |
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ddc 610 bkl 42.63 bkl 44.37 misc Airway epithelium misc Alveolar epithelium misc ENaC misc Ussing chamber misc Sodium absorption |
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Expression and function of the epithelial sodium channel δ-subunit in human respiratory epithelial cells in vitro |
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Expression and function of the epithelial sodium channel δ-subunit in human respiratory epithelial cells in vitro |
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Schwagerus, Elena |
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Schwagerus, Elena Sladek, Svenja Buckley, Stephen T. Armas-Capote, Natalia de la Rosa, Diego Alvarez Harvey, Brian J. Fischer, Horst Illek, Beate Huwer, Hanno Schneider-Daum, Nicole Lehr, Claus-Michael Ehrhardt, Carsten |
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expression and function of the epithelial sodium channel δ-subunit in human respiratory epithelial cells in vitro |
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Expression and function of the epithelial sodium channel δ-subunit in human respiratory epithelial cells in vitro |
| abstract |
Abstract Using human airway epithelial cell lines (i.e. NCI-H441 and Calu-3) as well as human alveolar epithelial type I-like (ATI) cells in primary culture, we studied the contribution of the epithelial sodium channel δ-subunit (δ-ENaC) to transepithelial sodium transport in human lung in vitro. Endogenous δ-ENaC protein was present in all three cell types tested; however, protein abundance was low, and no expression was detected in the apical cell membrane of these cells. Similarly, known modulators of δ-ENaC activity, such as capsazepine and icilin (activators) and Evans blue (inhibitor), did not show effects on short-circuit current (ISC), suggesting that δ-ENaC is not involved in the modulation of transcellular sodium absorption in NCI-H441 cell monolayers. Over-expression of δ-ENaC in NCI-H441 cells resulted in detectable protein expression in the apical cell membrane, as well as capsazepine and icilin-stimulated increases in ISC that were effectively blocked by Evans blue and that were consistent with δ-ENaC activation and inhibition, respectively. Consequently, these observations suggest that δ-ENaC expression is low in NCI-H441, Calu-3, and ATI cells and does not contribute to transepithelial sodium absorption. |
| abstractGer |
Abstract Using human airway epithelial cell lines (i.e. NCI-H441 and Calu-3) as well as human alveolar epithelial type I-like (ATI) cells in primary culture, we studied the contribution of the epithelial sodium channel δ-subunit (δ-ENaC) to transepithelial sodium transport in human lung in vitro. Endogenous δ-ENaC protein was present in all three cell types tested; however, protein abundance was low, and no expression was detected in the apical cell membrane of these cells. Similarly, known modulators of δ-ENaC activity, such as capsazepine and icilin (activators) and Evans blue (inhibitor), did not show effects on short-circuit current (ISC), suggesting that δ-ENaC is not involved in the modulation of transcellular sodium absorption in NCI-H441 cell monolayers. Over-expression of δ-ENaC in NCI-H441 cells resulted in detectable protein expression in the apical cell membrane, as well as capsazepine and icilin-stimulated increases in ISC that were effectively blocked by Evans blue and that were consistent with δ-ENaC activation and inhibition, respectively. Consequently, these observations suggest that δ-ENaC expression is low in NCI-H441, Calu-3, and ATI cells and does not contribute to transepithelial sodium absorption. |
| abstract_unstemmed |
Abstract Using human airway epithelial cell lines (i.e. NCI-H441 and Calu-3) as well as human alveolar epithelial type I-like (ATI) cells in primary culture, we studied the contribution of the epithelial sodium channel δ-subunit (δ-ENaC) to transepithelial sodium transport in human lung in vitro. Endogenous δ-ENaC protein was present in all three cell types tested; however, protein abundance was low, and no expression was detected in the apical cell membrane of these cells. Similarly, known modulators of δ-ENaC activity, such as capsazepine and icilin (activators) and Evans blue (inhibitor), did not show effects on short-circuit current (ISC), suggesting that δ-ENaC is not involved in the modulation of transcellular sodium absorption in NCI-H441 cell monolayers. Over-expression of δ-ENaC in NCI-H441 cells resulted in detectable protein expression in the apical cell membrane, as well as capsazepine and icilin-stimulated increases in ISC that were effectively blocked by Evans blue and that were consistent with δ-ENaC activation and inhibition, respectively. Consequently, these observations suggest that δ-ENaC expression is low in NCI-H441, Calu-3, and ATI cells and does not contribute to transepithelial sodium absorption. |
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Expression and function of the epithelial sodium channel δ-subunit in human respiratory epithelial cells in vitro |
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Sladek, Svenja Buckley, Stephen T. Armas-Capote, Natalia de la Rosa, Diego Alvarez Harvey, Brian J. Fischer, Horst Illek, Beate Huwer, Hanno Schneider-Daum, Nicole Lehr, Claus-Michael Ehrhardt, Carsten |
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| score |
7.3974285 |