Role of G protein-regulated inducer of neurite outgrowth 3 (GRIN3) in β-arrestin 2-Akt signaling and dopaminergic behaviors
Abstract The G protein-regulated inducer of neurite growth (GRIN) family has three isoforms (GRIN1-3), which bind to the Gαi/o subfamily of G protein that mediate signal processing via G protein-coupled receptors (GPCRs). Here, we show that GRIN3 is involved in regulation of dopamine-dependent behav...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Mototani, Yasumasa [verfasserIn] Okamura, Tadashi [verfasserIn] Goto, Motohito [verfasserIn] Shimizu, Yukiko [verfasserIn] Yanobu-Takanashi, Rieko [verfasserIn] Ito, Aiko [verfasserIn] Kawamura, Naoya [verfasserIn] Yagisawa, Yuka [verfasserIn] Umeki, Daisuke [verfasserIn] Nariyama, Megumi [verfasserIn] Suita, Kenji [verfasserIn] Ohnuki, Yoshiki [verfasserIn] Shiozawa, Kouichi [verfasserIn] Sahara, Yoshinori [verfasserIn] Kozasa, Tohru [verfasserIn] Saeki, Yasutake [verfasserIn] Okumura, Satoshi [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2018 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Pflügers Archiv - Berlin : Springer, 1868, 470(2018), 6 vom: 02. März, Seite 937-947 |
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| Übergeordnetes Werk: |
volume:470 ; year:2018 ; number:6 ; day:02 ; month:03 ; pages:937-947 |
| Links: |
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| DOI / URN: |
10.1007/s00424-018-2124-1 |
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| Katalog-ID: |
SPR005627788 |
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| 100 | 1 | |a Mototani, Yasumasa |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Role of G protein-regulated inducer of neurite outgrowth 3 (GRIN3) in β-arrestin 2-Akt signaling and dopaminergic behaviors |
| 264 | 1 | |c 2018 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
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| 520 | |a Abstract The G protein-regulated inducer of neurite growth (GRIN) family has three isoforms (GRIN1-3), which bind to the Gαi/o subfamily of G protein that mediate signal processing via G protein-coupled receptors (GPCRs). Here, we show that GRIN3 is involved in regulation of dopamine-dependent behaviors and is essential for activation of the dopamine receptors (DAR)-β-arrestin signaling cascade. Analysis of functional regions of GRIN3 showed that a di-cysteine motif (Cys751/752) is required for plasma membrane localization. GRIN3 was co-immunoprecipitated with GPCR kinases 2/6 and β-arrestins 1/2. Among GRINs, only GRIN3, which is highly expressed in striatum, strongly interacted with β-arrestin 2. We also generated GRIN3-knockout mice (GRIN3KO). GRIN3KO exhibited reduced locomotor activity and increased anxiety-like behavior in the elevated maze test, as well as a reduced locomoter response to dopamine stimulation. We also examined the phosphorylation of Akt at threonine 308 (phospho308-Akt), which is dephosphorylated via a β-arrestin 2-mediated pathway. Dephosphorylation of phospho308-Akt via the D2R-β-arrestin 2 signaling pathway was completely abolished in striatum of GRIN3KO. Our results suggest that GRIN3 has a role in recruitment and assembly of proteins involved in β-arrestin-dependent, G protein-independent signaling. | ||
| 650 | 4 | |a GRIN3 |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Dopamine receptor |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a β-Arrestin |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Akt |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a GRIN3-knockout mice |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Okamura, Tadashi |e verfasserin |4 aut | |
| 700 | 1 | |a Goto, Motohito |e verfasserin |4 aut | |
| 700 | 1 | |a Shimizu, Yukiko |e verfasserin |4 aut | |
| 700 | 1 | |a Yanobu-Takanashi, Rieko |e verfasserin |4 aut | |
| 700 | 1 | |a Ito, Aiko |e verfasserin |4 aut | |
| 700 | 1 | |a Kawamura, Naoya |e verfasserin |4 aut | |
| 700 | 1 | |a Yagisawa, Yuka |e verfasserin |4 aut | |
| 700 | 1 | |a Umeki, Daisuke |e verfasserin |4 aut | |
| 700 | 1 | |a Nariyama, Megumi |e verfasserin |4 aut | |
| 700 | 1 | |a Suita, Kenji |e verfasserin |4 aut | |
| 700 | 1 | |a Ohnuki, Yoshiki |e verfasserin |4 aut | |
| 700 | 1 | |a Shiozawa, Kouichi |e verfasserin |4 aut | |
| 700 | 1 | |a Sahara, Yoshinori |e verfasserin |4 aut | |
| 700 | 1 | |a Kozasa, Tohru |e verfasserin |4 aut | |
| 700 | 1 | |a Saeki, Yasutake |e verfasserin |4 aut | |
| 700 | 1 | |a Okumura, Satoshi |e verfasserin |4 aut | |
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10.1007/s00424-018-2124-1 doi (DE-627)SPR005627788 (SPR)s00424-018-2124-1-e DE-627 ger DE-627 rakwb eng 610 ASE 610 590 ASE 42.63 bkl 44.37 bkl Mototani, Yasumasa verfasserin aut Role of G protein-regulated inducer of neurite outgrowth 3 (GRIN3) in β-arrestin 2-Akt signaling and dopaminergic behaviors 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The G protein-regulated inducer of neurite growth (GRIN) family has three isoforms (GRIN1-3), which bind to the Gαi/o subfamily of G protein that mediate signal processing via G protein-coupled receptors (GPCRs). Here, we show that GRIN3 is involved in regulation of dopamine-dependent behaviors and is essential for activation of the dopamine receptors (DAR)-β-arrestin signaling cascade. Analysis of functional regions of GRIN3 showed that a di-cysteine motif (Cys751/752) is required for plasma membrane localization. GRIN3 was co-immunoprecipitated with GPCR kinases 2/6 and β-arrestins 1/2. Among GRINs, only GRIN3, which is highly expressed in striatum, strongly interacted with β-arrestin 2. We also generated GRIN3-knockout mice (GRIN3KO). GRIN3KO exhibited reduced locomotor activity and increased anxiety-like behavior in the elevated maze test, as well as a reduced locomoter response to dopamine stimulation. We also examined the phosphorylation of Akt at threonine 308 (phospho308-Akt), which is dephosphorylated via a β-arrestin 2-mediated pathway. Dephosphorylation of phospho308-Akt via the D2R-β-arrestin 2 signaling pathway was completely abolished in striatum of GRIN3KO. Our results suggest that GRIN3 has a role in recruitment and assembly of proteins involved in β-arrestin-dependent, G protein-independent signaling. GRIN3 (dpeaa)DE-He213 Dopamine receptor (dpeaa)DE-He213 β-Arrestin (dpeaa)DE-He213 Akt (dpeaa)DE-He213 GRIN3-knockout mice (dpeaa)DE-He213 Okamura, Tadashi verfasserin aut Goto, Motohito verfasserin aut Shimizu, Yukiko verfasserin aut Yanobu-Takanashi, Rieko verfasserin aut Ito, Aiko verfasserin aut Kawamura, Naoya verfasserin aut Yagisawa, Yuka verfasserin aut Umeki, Daisuke verfasserin aut Nariyama, Megumi verfasserin aut Suita, Kenji verfasserin aut Ohnuki, Yoshiki verfasserin aut Shiozawa, Kouichi verfasserin aut Sahara, Yoshinori verfasserin aut Kozasa, Tohru verfasserin aut Saeki, Yasutake verfasserin aut Okumura, Satoshi verfasserin aut Enthalten in Pflügers Archiv Berlin : Springer, 1868 470(2018), 6 vom: 02. März, Seite 937-947 (DE-627)25463897X (DE-600)1463014-X 1432-2013 nnns volume:470 year:2018 number:6 day:02 month:03 pages:937-947 https://dx.doi.org/10.1007/s00424-018-2124-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.63 ASE 44.37 ASE AR 470 2018 6 02 03 937-947 |
| spelling |
10.1007/s00424-018-2124-1 doi (DE-627)SPR005627788 (SPR)s00424-018-2124-1-e DE-627 ger DE-627 rakwb eng 610 ASE 610 590 ASE 42.63 bkl 44.37 bkl Mototani, Yasumasa verfasserin aut Role of G protein-regulated inducer of neurite outgrowth 3 (GRIN3) in β-arrestin 2-Akt signaling and dopaminergic behaviors 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The G protein-regulated inducer of neurite growth (GRIN) family has three isoforms (GRIN1-3), which bind to the Gαi/o subfamily of G protein that mediate signal processing via G protein-coupled receptors (GPCRs). Here, we show that GRIN3 is involved in regulation of dopamine-dependent behaviors and is essential for activation of the dopamine receptors (DAR)-β-arrestin signaling cascade. Analysis of functional regions of GRIN3 showed that a di-cysteine motif (Cys751/752) is required for plasma membrane localization. GRIN3 was co-immunoprecipitated with GPCR kinases 2/6 and β-arrestins 1/2. Among GRINs, only GRIN3, which is highly expressed in striatum, strongly interacted with β-arrestin 2. We also generated GRIN3-knockout mice (GRIN3KO). GRIN3KO exhibited reduced locomotor activity and increased anxiety-like behavior in the elevated maze test, as well as a reduced locomoter response to dopamine stimulation. We also examined the phosphorylation of Akt at threonine 308 (phospho308-Akt), which is dephosphorylated via a β-arrestin 2-mediated pathway. Dephosphorylation of phospho308-Akt via the D2R-β-arrestin 2 signaling pathway was completely abolished in striatum of GRIN3KO. Our results suggest that GRIN3 has a role in recruitment and assembly of proteins involved in β-arrestin-dependent, G protein-independent signaling. GRIN3 (dpeaa)DE-He213 Dopamine receptor (dpeaa)DE-He213 β-Arrestin (dpeaa)DE-He213 Akt (dpeaa)DE-He213 GRIN3-knockout mice (dpeaa)DE-He213 Okamura, Tadashi verfasserin aut Goto, Motohito verfasserin aut Shimizu, Yukiko verfasserin aut Yanobu-Takanashi, Rieko verfasserin aut Ito, Aiko verfasserin aut Kawamura, Naoya verfasserin aut Yagisawa, Yuka verfasserin aut Umeki, Daisuke verfasserin aut Nariyama, Megumi verfasserin aut Suita, Kenji verfasserin aut Ohnuki, Yoshiki verfasserin aut Shiozawa, Kouichi verfasserin aut Sahara, Yoshinori verfasserin aut Kozasa, Tohru verfasserin aut Saeki, Yasutake verfasserin aut Okumura, Satoshi verfasserin aut Enthalten in Pflügers Archiv Berlin : Springer, 1868 470(2018), 6 vom: 02. März, Seite 937-947 (DE-627)25463897X (DE-600)1463014-X 1432-2013 nnns volume:470 year:2018 number:6 day:02 month:03 pages:937-947 https://dx.doi.org/10.1007/s00424-018-2124-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.63 ASE 44.37 ASE AR 470 2018 6 02 03 937-947 |
| allfields_unstemmed |
10.1007/s00424-018-2124-1 doi (DE-627)SPR005627788 (SPR)s00424-018-2124-1-e DE-627 ger DE-627 rakwb eng 610 ASE 610 590 ASE 42.63 bkl 44.37 bkl Mototani, Yasumasa verfasserin aut Role of G protein-regulated inducer of neurite outgrowth 3 (GRIN3) in β-arrestin 2-Akt signaling and dopaminergic behaviors 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The G protein-regulated inducer of neurite growth (GRIN) family has three isoforms (GRIN1-3), which bind to the Gαi/o subfamily of G protein that mediate signal processing via G protein-coupled receptors (GPCRs). Here, we show that GRIN3 is involved in regulation of dopamine-dependent behaviors and is essential for activation of the dopamine receptors (DAR)-β-arrestin signaling cascade. Analysis of functional regions of GRIN3 showed that a di-cysteine motif (Cys751/752) is required for plasma membrane localization. GRIN3 was co-immunoprecipitated with GPCR kinases 2/6 and β-arrestins 1/2. Among GRINs, only GRIN3, which is highly expressed in striatum, strongly interacted with β-arrestin 2. We also generated GRIN3-knockout mice (GRIN3KO). GRIN3KO exhibited reduced locomotor activity and increased anxiety-like behavior in the elevated maze test, as well as a reduced locomoter response to dopamine stimulation. We also examined the phosphorylation of Akt at threonine 308 (phospho308-Akt), which is dephosphorylated via a β-arrestin 2-mediated pathway. Dephosphorylation of phospho308-Akt via the D2R-β-arrestin 2 signaling pathway was completely abolished in striatum of GRIN3KO. Our results suggest that GRIN3 has a role in recruitment and assembly of proteins involved in β-arrestin-dependent, G protein-independent signaling. GRIN3 (dpeaa)DE-He213 Dopamine receptor (dpeaa)DE-He213 β-Arrestin (dpeaa)DE-He213 Akt (dpeaa)DE-He213 GRIN3-knockout mice (dpeaa)DE-He213 Okamura, Tadashi verfasserin aut Goto, Motohito verfasserin aut Shimizu, Yukiko verfasserin aut Yanobu-Takanashi, Rieko verfasserin aut Ito, Aiko verfasserin aut Kawamura, Naoya verfasserin aut Yagisawa, Yuka verfasserin aut Umeki, Daisuke verfasserin aut Nariyama, Megumi verfasserin aut Suita, Kenji verfasserin aut Ohnuki, Yoshiki verfasserin aut Shiozawa, Kouichi verfasserin aut Sahara, Yoshinori verfasserin aut Kozasa, Tohru verfasserin aut Saeki, Yasutake verfasserin aut Okumura, Satoshi verfasserin aut Enthalten in Pflügers Archiv Berlin : Springer, 1868 470(2018), 6 vom: 02. März, Seite 937-947 (DE-627)25463897X (DE-600)1463014-X 1432-2013 nnns volume:470 year:2018 number:6 day:02 month:03 pages:937-947 https://dx.doi.org/10.1007/s00424-018-2124-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.63 ASE 44.37 ASE AR 470 2018 6 02 03 937-947 |
| allfieldsGer |
10.1007/s00424-018-2124-1 doi (DE-627)SPR005627788 (SPR)s00424-018-2124-1-e DE-627 ger DE-627 rakwb eng 610 ASE 610 590 ASE 42.63 bkl 44.37 bkl Mototani, Yasumasa verfasserin aut Role of G protein-regulated inducer of neurite outgrowth 3 (GRIN3) in β-arrestin 2-Akt signaling and dopaminergic behaviors 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The G protein-regulated inducer of neurite growth (GRIN) family has three isoforms (GRIN1-3), which bind to the Gαi/o subfamily of G protein that mediate signal processing via G protein-coupled receptors (GPCRs). Here, we show that GRIN3 is involved in regulation of dopamine-dependent behaviors and is essential for activation of the dopamine receptors (DAR)-β-arrestin signaling cascade. Analysis of functional regions of GRIN3 showed that a di-cysteine motif (Cys751/752) is required for plasma membrane localization. GRIN3 was co-immunoprecipitated with GPCR kinases 2/6 and β-arrestins 1/2. Among GRINs, only GRIN3, which is highly expressed in striatum, strongly interacted with β-arrestin 2. We also generated GRIN3-knockout mice (GRIN3KO). GRIN3KO exhibited reduced locomotor activity and increased anxiety-like behavior in the elevated maze test, as well as a reduced locomoter response to dopamine stimulation. We also examined the phosphorylation of Akt at threonine 308 (phospho308-Akt), which is dephosphorylated via a β-arrestin 2-mediated pathway. Dephosphorylation of phospho308-Akt via the D2R-β-arrestin 2 signaling pathway was completely abolished in striatum of GRIN3KO. Our results suggest that GRIN3 has a role in recruitment and assembly of proteins involved in β-arrestin-dependent, G protein-independent signaling. GRIN3 (dpeaa)DE-He213 Dopamine receptor (dpeaa)DE-He213 β-Arrestin (dpeaa)DE-He213 Akt (dpeaa)DE-He213 GRIN3-knockout mice (dpeaa)DE-He213 Okamura, Tadashi verfasserin aut Goto, Motohito verfasserin aut Shimizu, Yukiko verfasserin aut Yanobu-Takanashi, Rieko verfasserin aut Ito, Aiko verfasserin aut Kawamura, Naoya verfasserin aut Yagisawa, Yuka verfasserin aut Umeki, Daisuke verfasserin aut Nariyama, Megumi verfasserin aut Suita, Kenji verfasserin aut Ohnuki, Yoshiki verfasserin aut Shiozawa, Kouichi verfasserin aut Sahara, Yoshinori verfasserin aut Kozasa, Tohru verfasserin aut Saeki, Yasutake verfasserin aut Okumura, Satoshi verfasserin aut Enthalten in Pflügers Archiv Berlin : Springer, 1868 470(2018), 6 vom: 02. März, Seite 937-947 (DE-627)25463897X (DE-600)1463014-X 1432-2013 nnns volume:470 year:2018 number:6 day:02 month:03 pages:937-947 https://dx.doi.org/10.1007/s00424-018-2124-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.63 ASE 44.37 ASE AR 470 2018 6 02 03 937-947 |
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10.1007/s00424-018-2124-1 doi (DE-627)SPR005627788 (SPR)s00424-018-2124-1-e DE-627 ger DE-627 rakwb eng 610 ASE 610 590 ASE 42.63 bkl 44.37 bkl Mototani, Yasumasa verfasserin aut Role of G protein-regulated inducer of neurite outgrowth 3 (GRIN3) in β-arrestin 2-Akt signaling and dopaminergic behaviors 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The G protein-regulated inducer of neurite growth (GRIN) family has three isoforms (GRIN1-3), which bind to the Gαi/o subfamily of G protein that mediate signal processing via G protein-coupled receptors (GPCRs). Here, we show that GRIN3 is involved in regulation of dopamine-dependent behaviors and is essential for activation of the dopamine receptors (DAR)-β-arrestin signaling cascade. Analysis of functional regions of GRIN3 showed that a di-cysteine motif (Cys751/752) is required for plasma membrane localization. GRIN3 was co-immunoprecipitated with GPCR kinases 2/6 and β-arrestins 1/2. Among GRINs, only GRIN3, which is highly expressed in striatum, strongly interacted with β-arrestin 2. We also generated GRIN3-knockout mice (GRIN3KO). GRIN3KO exhibited reduced locomotor activity and increased anxiety-like behavior in the elevated maze test, as well as a reduced locomoter response to dopamine stimulation. We also examined the phosphorylation of Akt at threonine 308 (phospho308-Akt), which is dephosphorylated via a β-arrestin 2-mediated pathway. Dephosphorylation of phospho308-Akt via the D2R-β-arrestin 2 signaling pathway was completely abolished in striatum of GRIN3KO. Our results suggest that GRIN3 has a role in recruitment and assembly of proteins involved in β-arrestin-dependent, G protein-independent signaling. GRIN3 (dpeaa)DE-He213 Dopamine receptor (dpeaa)DE-He213 β-Arrestin (dpeaa)DE-He213 Akt (dpeaa)DE-He213 GRIN3-knockout mice (dpeaa)DE-He213 Okamura, Tadashi verfasserin aut Goto, Motohito verfasserin aut Shimizu, Yukiko verfasserin aut Yanobu-Takanashi, Rieko verfasserin aut Ito, Aiko verfasserin aut Kawamura, Naoya verfasserin aut Yagisawa, Yuka verfasserin aut Umeki, Daisuke verfasserin aut Nariyama, Megumi verfasserin aut Suita, Kenji verfasserin aut Ohnuki, Yoshiki verfasserin aut Shiozawa, Kouichi verfasserin aut Sahara, Yoshinori verfasserin aut Kozasa, Tohru verfasserin aut Saeki, Yasutake verfasserin aut Okumura, Satoshi verfasserin aut Enthalten in Pflügers Archiv Berlin : Springer, 1868 470(2018), 6 vom: 02. März, Seite 937-947 (DE-627)25463897X (DE-600)1463014-X 1432-2013 nnns volume:470 year:2018 number:6 day:02 month:03 pages:937-947 https://dx.doi.org/10.1007/s00424-018-2124-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.63 ASE 44.37 ASE AR 470 2018 6 02 03 937-947 |
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Enthalten in Pflügers Archiv 470(2018), 6 vom: 02. März, Seite 937-947 volume:470 year:2018 number:6 day:02 month:03 pages:937-947 |
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GRIN3 Dopamine receptor β-Arrestin Akt GRIN3-knockout mice |
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Mototani, Yasumasa @@aut@@ Okamura, Tadashi @@aut@@ Goto, Motohito @@aut@@ Shimizu, Yukiko @@aut@@ Yanobu-Takanashi, Rieko @@aut@@ Ito, Aiko @@aut@@ Kawamura, Naoya @@aut@@ Yagisawa, Yuka @@aut@@ Umeki, Daisuke @@aut@@ Nariyama, Megumi @@aut@@ Suita, Kenji @@aut@@ Ohnuki, Yoshiki @@aut@@ Shiozawa, Kouichi @@aut@@ Sahara, Yoshinori @@aut@@ Kozasa, Tohru @@aut@@ Saeki, Yasutake @@aut@@ Okumura, Satoshi @@aut@@ |
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2018-03-02T00:00:00Z |
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englisch |
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|
| author |
Mototani, Yasumasa |
| spellingShingle |
Mototani, Yasumasa ddc 610 bkl 42.63 bkl 44.37 misc GRIN3 misc Dopamine receptor misc β-Arrestin misc Akt misc GRIN3-knockout mice Role of G protein-regulated inducer of neurite outgrowth 3 (GRIN3) in β-arrestin 2-Akt signaling and dopaminergic behaviors |
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1432-2013 |
| topic_title |
610 ASE 610 590 ASE 42.63 bkl 44.37 bkl Role of G protein-regulated inducer of neurite outgrowth 3 (GRIN3) in β-arrestin 2-Akt signaling and dopaminergic behaviors GRIN3 (dpeaa)DE-He213 Dopamine receptor (dpeaa)DE-He213 β-Arrestin (dpeaa)DE-He213 Akt (dpeaa)DE-He213 GRIN3-knockout mice (dpeaa)DE-He213 |
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ddc 610 bkl 42.63 bkl 44.37 misc GRIN3 misc Dopamine receptor misc β-Arrestin misc Akt misc GRIN3-knockout mice |
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ddc 610 bkl 42.63 bkl 44.37 misc GRIN3 misc Dopamine receptor misc β-Arrestin misc Akt misc GRIN3-knockout mice |
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ddc 610 bkl 42.63 bkl 44.37 misc GRIN3 misc Dopamine receptor misc β-Arrestin misc Akt misc GRIN3-knockout mice |
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Role of G protein-regulated inducer of neurite outgrowth 3 (GRIN3) in β-arrestin 2-Akt signaling and dopaminergic behaviors |
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(DE-627)SPR005627788 (SPR)s00424-018-2124-1-e |
| title_full |
Role of G protein-regulated inducer of neurite outgrowth 3 (GRIN3) in β-arrestin 2-Akt signaling and dopaminergic behaviors |
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Mototani, Yasumasa |
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Mototani, Yasumasa Okamura, Tadashi Goto, Motohito Shimizu, Yukiko Yanobu-Takanashi, Rieko Ito, Aiko Kawamura, Naoya Yagisawa, Yuka Umeki, Daisuke Nariyama, Megumi Suita, Kenji Ohnuki, Yoshiki Shiozawa, Kouichi Sahara, Yoshinori Kozasa, Tohru Saeki, Yasutake Okumura, Satoshi |
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Elektronische Aufsätze |
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Mototani, Yasumasa |
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10.1007/s00424-018-2124-1 |
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610 590 |
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verfasserin |
| title_sort |
role of g protein-regulated inducer of neurite outgrowth 3 (grin3) in β-arrestin 2-akt signaling and dopaminergic behaviors |
| title_auth |
Role of G protein-regulated inducer of neurite outgrowth 3 (GRIN3) in β-arrestin 2-Akt signaling and dopaminergic behaviors |
| abstract |
Abstract The G protein-regulated inducer of neurite growth (GRIN) family has three isoforms (GRIN1-3), which bind to the Gαi/o subfamily of G protein that mediate signal processing via G protein-coupled receptors (GPCRs). Here, we show that GRIN3 is involved in regulation of dopamine-dependent behaviors and is essential for activation of the dopamine receptors (DAR)-β-arrestin signaling cascade. Analysis of functional regions of GRIN3 showed that a di-cysteine motif (Cys751/752) is required for plasma membrane localization. GRIN3 was co-immunoprecipitated with GPCR kinases 2/6 and β-arrestins 1/2. Among GRINs, only GRIN3, which is highly expressed in striatum, strongly interacted with β-arrestin 2. We also generated GRIN3-knockout mice (GRIN3KO). GRIN3KO exhibited reduced locomotor activity and increased anxiety-like behavior in the elevated maze test, as well as a reduced locomoter response to dopamine stimulation. We also examined the phosphorylation of Akt at threonine 308 (phospho308-Akt), which is dephosphorylated via a β-arrestin 2-mediated pathway. Dephosphorylation of phospho308-Akt via the D2R-β-arrestin 2 signaling pathway was completely abolished in striatum of GRIN3KO. Our results suggest that GRIN3 has a role in recruitment and assembly of proteins involved in β-arrestin-dependent, G protein-independent signaling. |
| abstractGer |
Abstract The G protein-regulated inducer of neurite growth (GRIN) family has three isoforms (GRIN1-3), which bind to the Gαi/o subfamily of G protein that mediate signal processing via G protein-coupled receptors (GPCRs). Here, we show that GRIN3 is involved in regulation of dopamine-dependent behaviors and is essential for activation of the dopamine receptors (DAR)-β-arrestin signaling cascade. Analysis of functional regions of GRIN3 showed that a di-cysteine motif (Cys751/752) is required for plasma membrane localization. GRIN3 was co-immunoprecipitated with GPCR kinases 2/6 and β-arrestins 1/2. Among GRINs, only GRIN3, which is highly expressed in striatum, strongly interacted with β-arrestin 2. We also generated GRIN3-knockout mice (GRIN3KO). GRIN3KO exhibited reduced locomotor activity and increased anxiety-like behavior in the elevated maze test, as well as a reduced locomoter response to dopamine stimulation. We also examined the phosphorylation of Akt at threonine 308 (phospho308-Akt), which is dephosphorylated via a β-arrestin 2-mediated pathway. Dephosphorylation of phospho308-Akt via the D2R-β-arrestin 2 signaling pathway was completely abolished in striatum of GRIN3KO. Our results suggest that GRIN3 has a role in recruitment and assembly of proteins involved in β-arrestin-dependent, G protein-independent signaling. |
| abstract_unstemmed |
Abstract The G protein-regulated inducer of neurite growth (GRIN) family has three isoforms (GRIN1-3), which bind to the Gαi/o subfamily of G protein that mediate signal processing via G protein-coupled receptors (GPCRs). Here, we show that GRIN3 is involved in regulation of dopamine-dependent behaviors and is essential for activation of the dopamine receptors (DAR)-β-arrestin signaling cascade. Analysis of functional regions of GRIN3 showed that a di-cysteine motif (Cys751/752) is required for plasma membrane localization. GRIN3 was co-immunoprecipitated with GPCR kinases 2/6 and β-arrestins 1/2. Among GRINs, only GRIN3, which is highly expressed in striatum, strongly interacted with β-arrestin 2. We also generated GRIN3-knockout mice (GRIN3KO). GRIN3KO exhibited reduced locomotor activity and increased anxiety-like behavior in the elevated maze test, as well as a reduced locomoter response to dopamine stimulation. We also examined the phosphorylation of Akt at threonine 308 (phospho308-Akt), which is dephosphorylated via a β-arrestin 2-mediated pathway. Dephosphorylation of phospho308-Akt via the D2R-β-arrestin 2 signaling pathway was completely abolished in striatum of GRIN3KO. Our results suggest that GRIN3 has a role in recruitment and assembly of proteins involved in β-arrestin-dependent, G protein-independent signaling. |
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| title_short |
Role of G protein-regulated inducer of neurite outgrowth 3 (GRIN3) in β-arrestin 2-Akt signaling and dopaminergic behaviors |
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https://dx.doi.org/10.1007/s00424-018-2124-1 |
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Okamura, Tadashi Goto, Motohito Shimizu, Yukiko Yanobu-Takanashi, Rieko Ito, Aiko Kawamura, Naoya Yagisawa, Yuka Umeki, Daisuke Nariyama, Megumi Suita, Kenji Ohnuki, Yoshiki Shiozawa, Kouichi Sahara, Yoshinori Kozasa, Tohru Saeki, Yasutake Okumura, Satoshi |
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Okamura, Tadashi Goto, Motohito Shimizu, Yukiko Yanobu-Takanashi, Rieko Ito, Aiko Kawamura, Naoya Yagisawa, Yuka Umeki, Daisuke Nariyama, Megumi Suita, Kenji Ohnuki, Yoshiki Shiozawa, Kouichi Sahara, Yoshinori Kozasa, Tohru Saeki, Yasutake Okumura, Satoshi |
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10.1007/s00424-018-2124-1 |
| up_date |
2024-07-03T17:37:45.876Z |
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| score |
7.398855 |