The interaction domains of the plant Myc-like bHLH transcription factors can regulate the transactivation strength
Abstract The N-terminal region of the plant Myc-like basic helix–loop–helix transcription factors (bHLH TFs) contains two domains. Approximately, 190 amino acids at the N-terminus comprise an interaction domain, a.k.a. Myb-interacting-region (MIR) for its primary function of interacting with Myb-lik...
Ausführliche Beschreibung
Autor*in: |
Pattanaik, Sitakanta [verfasserIn] Xie, Claire H. [verfasserIn] Yuan, Ling [verfasserIn] |
---|
Format: |
E-Artikel |
---|---|
Sprache: |
Englisch |
Erschienen: |
2007 |
---|
Schlagwörter: |
---|
Übergeordnetes Werk: |
Enthalten in: Planta - Berlin : Springer, 1925, 227(2007), 3 vom: 13. Dez., Seite 707-715 |
---|---|
Übergeordnetes Werk: |
volume:227 ; year:2007 ; number:3 ; day:13 ; month:12 ; pages:707-715 |
Links: |
---|
DOI / URN: |
10.1007/s00425-007-0676-y |
---|
Katalog-ID: |
SPR005654572 |
---|
LEADER | 01000caa a22002652 4500 | ||
---|---|---|---|
001 | SPR005654572 | ||
003 | DE-627 | ||
005 | 20230520001614.0 | ||
007 | cr uuu---uuuuu | ||
008 | 201002s2007 xx |||||o 00| ||eng c | ||
024 | 7 | |a 10.1007/s00425-007-0676-y |2 doi | |
035 | |a (DE-627)SPR005654572 | ||
035 | |a (SPR)s00425-007-0676-y-e | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
082 | 0 | 4 | |a 580 |q ASE |
084 | |a 42.38 |2 bkl | ||
100 | 1 | |a Pattanaik, Sitakanta |e verfasserin |4 aut | |
245 | 1 | 4 | |a The interaction domains of the plant Myc-like bHLH transcription factors can regulate the transactivation strength |
264 | 1 | |c 2007 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a Computermedien |b c |2 rdamedia | ||
338 | |a Online-Ressource |b cr |2 rdacarrier | ||
520 | |a Abstract The N-terminal region of the plant Myc-like basic helix–loop–helix transcription factors (bHLH TFs) contains two domains. Approximately, 190 amino acids at the N-terminus comprise an interaction domain, a.k.a. Myb-interacting-region (MIR) for its primary function of interacting with Myb-like TFs. Following, the interaction domain is an activation (or acidic) domain responsible for transactivation. We have previously discovered that a lysine to methionine substitution (K157M) in the interaction domain of Myc-RP of Perilla frutescens leads to a 50-fold increase in transactivation activity. The result suggests that mutations in the interaction domain affect transactivation. The highly conserved nature of this lysine residue in many Myc-like bHLH TFs prompted us to explore the functional importance of this residue within the TF family and the influence of the interaction domain on the activation domain in transactivation. We found that the replacement of the equivalent lysine with methionine significantly affects the transactivation activities of two other Myc-RP homologues, Delila from snapdragon and Lc from maize. In addition to methionine, substitution with several other amino acids at this position has positive effects on transcriptional activity. A neighboring conserved alanine residue (A159 in Myc-RP, A161 in Delila and A172 in Lc) also affects transactivation. Substitution of this alanine residue to an aspartic acid abolished transactivation of both Myc-RP and Delila and severely reduced transactivation of Lc. Ectopic expression of a Myc-RP K157M mutant in transgenic tobacco resulted in increased anthocyanin accumulation compared to plants expressing the wild-type gene. Our study reveals the potential cooperation between functional domains of the bHLH TFs. | ||
650 | 4 | |a Basic helix–loop–helix transcription factors |7 (dpeaa)DE-He213 | |
650 | 4 | |a Transcription factor domains |7 (dpeaa)DE-He213 | |
650 | 4 | |a Directed evolution |7 (dpeaa)DE-He213 | |
650 | 4 | |a Anthocyanin |7 (dpeaa)DE-He213 | |
700 | 1 | |a Xie, Claire H. |e verfasserin |4 aut | |
700 | 1 | |a Yuan, Ling |e verfasserin |4 aut | |
773 | 0 | 8 | |i Enthalten in |t Planta |d Berlin : Springer, 1925 |g 227(2007), 3 vom: 13. Dez., Seite 707-715 |w (DE-627)254639100 |w (DE-600)1463030-8 |x 1432-2048 |7 nnns |
773 | 1 | 8 | |g volume:227 |g year:2007 |g number:3 |g day:13 |g month:12 |g pages:707-715 |
856 | 4 | 0 | |u https://dx.doi.org/10.1007/s00425-007-0676-y |z lizenzpflichtig |3 Volltext |
912 | |a GBV_USEFLAG_A | ||
912 | |a SYSFLAG_A | ||
912 | |a GBV_SPRINGER | ||
912 | |a SSG-OLC-PHA | ||
912 | |a GBV_ILN_11 | ||
912 | |a GBV_ILN_20 | ||
912 | |a GBV_ILN_22 | ||
912 | |a GBV_ILN_23 | ||
912 | |a GBV_ILN_24 | ||
912 | |a GBV_ILN_31 | ||
912 | |a GBV_ILN_32 | ||
912 | |a GBV_ILN_39 | ||
912 | |a GBV_ILN_40 | ||
912 | |a GBV_ILN_60 | ||
912 | |a GBV_ILN_62 | ||
912 | |a GBV_ILN_63 | ||
912 | |a GBV_ILN_65 | ||
912 | |a GBV_ILN_69 | ||
912 | |a GBV_ILN_70 | ||
912 | |a GBV_ILN_73 | ||
912 | |a GBV_ILN_74 | ||
912 | |a GBV_ILN_90 | ||
912 | |a GBV_ILN_95 | ||
912 | |a GBV_ILN_100 | ||
912 | |a GBV_ILN_105 | ||
912 | |a GBV_ILN_110 | ||
912 | |a GBV_ILN_120 | ||
912 | |a GBV_ILN_138 | ||
912 | |a GBV_ILN_150 | ||
912 | |a GBV_ILN_151 | ||
912 | |a GBV_ILN_152 | ||
912 | |a GBV_ILN_161 | ||
912 | |a GBV_ILN_170 | ||
912 | |a GBV_ILN_171 | ||
912 | |a GBV_ILN_187 | ||
912 | |a GBV_ILN_213 | ||
912 | |a GBV_ILN_224 | ||
912 | |a GBV_ILN_230 | ||
912 | |a GBV_ILN_250 | ||
912 | |a GBV_ILN_267 | ||
912 | |a GBV_ILN_281 | ||
912 | |a GBV_ILN_285 | ||
912 | |a GBV_ILN_293 | ||
912 | |a GBV_ILN_370 | ||
912 | |a GBV_ILN_374 | ||
912 | |a GBV_ILN_602 | ||
912 | |a GBV_ILN_636 | ||
912 | |a GBV_ILN_647 | ||
912 | |a GBV_ILN_702 | ||
912 | |a GBV_ILN_2001 | ||
912 | |a GBV_ILN_2003 | ||
912 | |a GBV_ILN_2004 | ||
912 | |a GBV_ILN_2005 | ||
912 | |a GBV_ILN_2006 | ||
912 | |a GBV_ILN_2007 | ||
912 | |a GBV_ILN_2008 | ||
912 | |a GBV_ILN_2009 | ||
912 | |a GBV_ILN_2010 | ||
912 | |a GBV_ILN_2011 | ||
912 | |a GBV_ILN_2014 | ||
912 | |a GBV_ILN_2015 | ||
912 | |a GBV_ILN_2018 | ||
912 | |a GBV_ILN_2020 | ||
912 | |a GBV_ILN_2021 | ||
912 | |a GBV_ILN_2025 | ||
912 | |a GBV_ILN_2026 | ||
912 | |a GBV_ILN_2027 | ||
912 | |a GBV_ILN_2031 | ||
912 | |a GBV_ILN_2034 | ||
912 | |a GBV_ILN_2037 | ||
912 | |a GBV_ILN_2038 | ||
912 | |a GBV_ILN_2039 | ||
912 | |a GBV_ILN_2044 | ||
912 | |a GBV_ILN_2048 | ||
912 | |a GBV_ILN_2049 | ||
912 | |a GBV_ILN_2050 | ||
912 | |a GBV_ILN_2055 | ||
912 | |a GBV_ILN_2057 | ||
912 | |a GBV_ILN_2059 | ||
912 | |a GBV_ILN_2061 | ||
912 | |a GBV_ILN_2064 | ||
912 | |a GBV_ILN_2065 | ||
912 | |a GBV_ILN_2068 | ||
912 | |a GBV_ILN_2070 | ||
912 | |a GBV_ILN_2086 | ||
912 | |a GBV_ILN_2088 | ||
912 | |a GBV_ILN_2093 | ||
912 | |a GBV_ILN_2106 | ||
912 | |a GBV_ILN_2107 | ||
912 | |a GBV_ILN_2108 | ||
912 | |a GBV_ILN_2110 | ||
912 | |a GBV_ILN_2111 | ||
912 | |a GBV_ILN_2112 | ||
912 | |a GBV_ILN_2113 | ||
912 | |a GBV_ILN_2116 | ||
912 | |a GBV_ILN_2118 | ||
912 | |a GBV_ILN_2119 | ||
912 | |a GBV_ILN_2122 | ||
912 | |a GBV_ILN_2129 | ||
912 | |a GBV_ILN_2143 | ||
912 | |a GBV_ILN_2144 | ||
912 | |a GBV_ILN_2147 | ||
912 | |a GBV_ILN_2148 | ||
912 | |a GBV_ILN_2152 | ||
912 | |a GBV_ILN_2153 | ||
912 | |a GBV_ILN_2188 | ||
912 | |a GBV_ILN_2190 | ||
912 | |a GBV_ILN_2232 | ||
912 | |a GBV_ILN_2336 | ||
912 | |a GBV_ILN_2446 | ||
912 | |a GBV_ILN_2470 | ||
912 | |a GBV_ILN_2472 | ||
912 | |a GBV_ILN_2507 | ||
912 | |a GBV_ILN_2522 | ||
912 | |a GBV_ILN_2548 | ||
912 | |a GBV_ILN_2946 | ||
912 | |a GBV_ILN_2949 | ||
912 | |a GBV_ILN_2951 | ||
912 | |a GBV_ILN_4012 | ||
912 | |a GBV_ILN_4035 | ||
912 | |a GBV_ILN_4037 | ||
912 | |a GBV_ILN_4046 | ||
912 | |a GBV_ILN_4112 | ||
912 | |a GBV_ILN_4125 | ||
912 | |a GBV_ILN_4126 | ||
912 | |a GBV_ILN_4242 | ||
912 | |a GBV_ILN_4246 | ||
912 | |a GBV_ILN_4249 | ||
912 | |a GBV_ILN_4251 | ||
912 | |a GBV_ILN_4305 | ||
912 | |a GBV_ILN_4306 | ||
912 | |a GBV_ILN_4307 | ||
912 | |a GBV_ILN_4313 | ||
912 | |a GBV_ILN_4322 | ||
912 | |a GBV_ILN_4323 | ||
912 | |a GBV_ILN_4324 | ||
912 | |a GBV_ILN_4325 | ||
912 | |a GBV_ILN_4326 | ||
912 | |a GBV_ILN_4328 | ||
912 | |a GBV_ILN_4333 | ||
912 | |a GBV_ILN_4334 | ||
912 | |a GBV_ILN_4335 | ||
912 | |a GBV_ILN_4336 | ||
912 | |a GBV_ILN_4338 | ||
912 | |a GBV_ILN_4346 | ||
912 | |a GBV_ILN_4393 | ||
912 | |a GBV_ILN_4700 | ||
936 | b | k | |a 42.38 |q ASE |
951 | |a AR | ||
952 | |d 227 |j 2007 |e 3 |b 13 |c 12 |h 707-715 |
author_variant |
s p sp c h x ch chx l y ly |
---|---|
matchkey_str |
article:14322048:2007----::hitrcinoanotelnmciehhrncitofcosarglt |
hierarchy_sort_str |
2007 |
bklnumber |
42.38 |
publishDate |
2007 |
allfields |
10.1007/s00425-007-0676-y doi (DE-627)SPR005654572 (SPR)s00425-007-0676-y-e DE-627 ger DE-627 rakwb eng 580 ASE 42.38 bkl Pattanaik, Sitakanta verfasserin aut The interaction domains of the plant Myc-like bHLH transcription factors can regulate the transactivation strength 2007 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The N-terminal region of the plant Myc-like basic helix–loop–helix transcription factors (bHLH TFs) contains two domains. Approximately, 190 amino acids at the N-terminus comprise an interaction domain, a.k.a. Myb-interacting-region (MIR) for its primary function of interacting with Myb-like TFs. Following, the interaction domain is an activation (or acidic) domain responsible for transactivation. We have previously discovered that a lysine to methionine substitution (K157M) in the interaction domain of Myc-RP of Perilla frutescens leads to a 50-fold increase in transactivation activity. The result suggests that mutations in the interaction domain affect transactivation. The highly conserved nature of this lysine residue in many Myc-like bHLH TFs prompted us to explore the functional importance of this residue within the TF family and the influence of the interaction domain on the activation domain in transactivation. We found that the replacement of the equivalent lysine with methionine significantly affects the transactivation activities of two other Myc-RP homologues, Delila from snapdragon and Lc from maize. In addition to methionine, substitution with several other amino acids at this position has positive effects on transcriptional activity. A neighboring conserved alanine residue (A159 in Myc-RP, A161 in Delila and A172 in Lc) also affects transactivation. Substitution of this alanine residue to an aspartic acid abolished transactivation of both Myc-RP and Delila and severely reduced transactivation of Lc. Ectopic expression of a Myc-RP K157M mutant in transgenic tobacco resulted in increased anthocyanin accumulation compared to plants expressing the wild-type gene. Our study reveals the potential cooperation between functional domains of the bHLH TFs. Basic helix–loop–helix transcription factors (dpeaa)DE-He213 Transcription factor domains (dpeaa)DE-He213 Directed evolution (dpeaa)DE-He213 Anthocyanin (dpeaa)DE-He213 Xie, Claire H. verfasserin aut Yuan, Ling verfasserin aut Enthalten in Planta Berlin : Springer, 1925 227(2007), 3 vom: 13. Dez., Seite 707-715 (DE-627)254639100 (DE-600)1463030-8 1432-2048 nnns volume:227 year:2007 number:3 day:13 month:12 pages:707-715 https://dx.doi.org/10.1007/s00425-007-0676-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2946 GBV_ILN_2949 GBV_ILN_2951 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4393 GBV_ILN_4700 42.38 ASE AR 227 2007 3 13 12 707-715 |
spelling |
10.1007/s00425-007-0676-y doi (DE-627)SPR005654572 (SPR)s00425-007-0676-y-e DE-627 ger DE-627 rakwb eng 580 ASE 42.38 bkl Pattanaik, Sitakanta verfasserin aut The interaction domains of the plant Myc-like bHLH transcription factors can regulate the transactivation strength 2007 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The N-terminal region of the plant Myc-like basic helix–loop–helix transcription factors (bHLH TFs) contains two domains. Approximately, 190 amino acids at the N-terminus comprise an interaction domain, a.k.a. Myb-interacting-region (MIR) for its primary function of interacting with Myb-like TFs. Following, the interaction domain is an activation (or acidic) domain responsible for transactivation. We have previously discovered that a lysine to methionine substitution (K157M) in the interaction domain of Myc-RP of Perilla frutescens leads to a 50-fold increase in transactivation activity. The result suggests that mutations in the interaction domain affect transactivation. The highly conserved nature of this lysine residue in many Myc-like bHLH TFs prompted us to explore the functional importance of this residue within the TF family and the influence of the interaction domain on the activation domain in transactivation. We found that the replacement of the equivalent lysine with methionine significantly affects the transactivation activities of two other Myc-RP homologues, Delila from snapdragon and Lc from maize. In addition to methionine, substitution with several other amino acids at this position has positive effects on transcriptional activity. A neighboring conserved alanine residue (A159 in Myc-RP, A161 in Delila and A172 in Lc) also affects transactivation. Substitution of this alanine residue to an aspartic acid abolished transactivation of both Myc-RP and Delila and severely reduced transactivation of Lc. Ectopic expression of a Myc-RP K157M mutant in transgenic tobacco resulted in increased anthocyanin accumulation compared to plants expressing the wild-type gene. Our study reveals the potential cooperation between functional domains of the bHLH TFs. Basic helix–loop–helix transcription factors (dpeaa)DE-He213 Transcription factor domains (dpeaa)DE-He213 Directed evolution (dpeaa)DE-He213 Anthocyanin (dpeaa)DE-He213 Xie, Claire H. verfasserin aut Yuan, Ling verfasserin aut Enthalten in Planta Berlin : Springer, 1925 227(2007), 3 vom: 13. Dez., Seite 707-715 (DE-627)254639100 (DE-600)1463030-8 1432-2048 nnns volume:227 year:2007 number:3 day:13 month:12 pages:707-715 https://dx.doi.org/10.1007/s00425-007-0676-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2946 GBV_ILN_2949 GBV_ILN_2951 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4393 GBV_ILN_4700 42.38 ASE AR 227 2007 3 13 12 707-715 |
allfields_unstemmed |
10.1007/s00425-007-0676-y doi (DE-627)SPR005654572 (SPR)s00425-007-0676-y-e DE-627 ger DE-627 rakwb eng 580 ASE 42.38 bkl Pattanaik, Sitakanta verfasserin aut The interaction domains of the plant Myc-like bHLH transcription factors can regulate the transactivation strength 2007 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The N-terminal region of the plant Myc-like basic helix–loop–helix transcription factors (bHLH TFs) contains two domains. Approximately, 190 amino acids at the N-terminus comprise an interaction domain, a.k.a. Myb-interacting-region (MIR) for its primary function of interacting with Myb-like TFs. Following, the interaction domain is an activation (or acidic) domain responsible for transactivation. We have previously discovered that a lysine to methionine substitution (K157M) in the interaction domain of Myc-RP of Perilla frutescens leads to a 50-fold increase in transactivation activity. The result suggests that mutations in the interaction domain affect transactivation. The highly conserved nature of this lysine residue in many Myc-like bHLH TFs prompted us to explore the functional importance of this residue within the TF family and the influence of the interaction domain on the activation domain in transactivation. We found that the replacement of the equivalent lysine with methionine significantly affects the transactivation activities of two other Myc-RP homologues, Delila from snapdragon and Lc from maize. In addition to methionine, substitution with several other amino acids at this position has positive effects on transcriptional activity. A neighboring conserved alanine residue (A159 in Myc-RP, A161 in Delila and A172 in Lc) also affects transactivation. Substitution of this alanine residue to an aspartic acid abolished transactivation of both Myc-RP and Delila and severely reduced transactivation of Lc. Ectopic expression of a Myc-RP K157M mutant in transgenic tobacco resulted in increased anthocyanin accumulation compared to plants expressing the wild-type gene. Our study reveals the potential cooperation between functional domains of the bHLH TFs. Basic helix–loop–helix transcription factors (dpeaa)DE-He213 Transcription factor domains (dpeaa)DE-He213 Directed evolution (dpeaa)DE-He213 Anthocyanin (dpeaa)DE-He213 Xie, Claire H. verfasserin aut Yuan, Ling verfasserin aut Enthalten in Planta Berlin : Springer, 1925 227(2007), 3 vom: 13. Dez., Seite 707-715 (DE-627)254639100 (DE-600)1463030-8 1432-2048 nnns volume:227 year:2007 number:3 day:13 month:12 pages:707-715 https://dx.doi.org/10.1007/s00425-007-0676-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2946 GBV_ILN_2949 GBV_ILN_2951 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4393 GBV_ILN_4700 42.38 ASE AR 227 2007 3 13 12 707-715 |
allfieldsGer |
10.1007/s00425-007-0676-y doi (DE-627)SPR005654572 (SPR)s00425-007-0676-y-e DE-627 ger DE-627 rakwb eng 580 ASE 42.38 bkl Pattanaik, Sitakanta verfasserin aut The interaction domains of the plant Myc-like bHLH transcription factors can regulate the transactivation strength 2007 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The N-terminal region of the plant Myc-like basic helix–loop–helix transcription factors (bHLH TFs) contains two domains. Approximately, 190 amino acids at the N-terminus comprise an interaction domain, a.k.a. Myb-interacting-region (MIR) for its primary function of interacting with Myb-like TFs. Following, the interaction domain is an activation (or acidic) domain responsible for transactivation. We have previously discovered that a lysine to methionine substitution (K157M) in the interaction domain of Myc-RP of Perilla frutescens leads to a 50-fold increase in transactivation activity. The result suggests that mutations in the interaction domain affect transactivation. The highly conserved nature of this lysine residue in many Myc-like bHLH TFs prompted us to explore the functional importance of this residue within the TF family and the influence of the interaction domain on the activation domain in transactivation. We found that the replacement of the equivalent lysine with methionine significantly affects the transactivation activities of two other Myc-RP homologues, Delila from snapdragon and Lc from maize. In addition to methionine, substitution with several other amino acids at this position has positive effects on transcriptional activity. A neighboring conserved alanine residue (A159 in Myc-RP, A161 in Delila and A172 in Lc) also affects transactivation. Substitution of this alanine residue to an aspartic acid abolished transactivation of both Myc-RP and Delila and severely reduced transactivation of Lc. Ectopic expression of a Myc-RP K157M mutant in transgenic tobacco resulted in increased anthocyanin accumulation compared to plants expressing the wild-type gene. Our study reveals the potential cooperation between functional domains of the bHLH TFs. Basic helix–loop–helix transcription factors (dpeaa)DE-He213 Transcription factor domains (dpeaa)DE-He213 Directed evolution (dpeaa)DE-He213 Anthocyanin (dpeaa)DE-He213 Xie, Claire H. verfasserin aut Yuan, Ling verfasserin aut Enthalten in Planta Berlin : Springer, 1925 227(2007), 3 vom: 13. Dez., Seite 707-715 (DE-627)254639100 (DE-600)1463030-8 1432-2048 nnns volume:227 year:2007 number:3 day:13 month:12 pages:707-715 https://dx.doi.org/10.1007/s00425-007-0676-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2946 GBV_ILN_2949 GBV_ILN_2951 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4393 GBV_ILN_4700 42.38 ASE AR 227 2007 3 13 12 707-715 |
allfieldsSound |
10.1007/s00425-007-0676-y doi (DE-627)SPR005654572 (SPR)s00425-007-0676-y-e DE-627 ger DE-627 rakwb eng 580 ASE 42.38 bkl Pattanaik, Sitakanta verfasserin aut The interaction domains of the plant Myc-like bHLH transcription factors can regulate the transactivation strength 2007 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The N-terminal region of the plant Myc-like basic helix–loop–helix transcription factors (bHLH TFs) contains two domains. Approximately, 190 amino acids at the N-terminus comprise an interaction domain, a.k.a. Myb-interacting-region (MIR) for its primary function of interacting with Myb-like TFs. Following, the interaction domain is an activation (or acidic) domain responsible for transactivation. We have previously discovered that a lysine to methionine substitution (K157M) in the interaction domain of Myc-RP of Perilla frutescens leads to a 50-fold increase in transactivation activity. The result suggests that mutations in the interaction domain affect transactivation. The highly conserved nature of this lysine residue in many Myc-like bHLH TFs prompted us to explore the functional importance of this residue within the TF family and the influence of the interaction domain on the activation domain in transactivation. We found that the replacement of the equivalent lysine with methionine significantly affects the transactivation activities of two other Myc-RP homologues, Delila from snapdragon and Lc from maize. In addition to methionine, substitution with several other amino acids at this position has positive effects on transcriptional activity. A neighboring conserved alanine residue (A159 in Myc-RP, A161 in Delila and A172 in Lc) also affects transactivation. Substitution of this alanine residue to an aspartic acid abolished transactivation of both Myc-RP and Delila and severely reduced transactivation of Lc. Ectopic expression of a Myc-RP K157M mutant in transgenic tobacco resulted in increased anthocyanin accumulation compared to plants expressing the wild-type gene. Our study reveals the potential cooperation between functional domains of the bHLH TFs. Basic helix–loop–helix transcription factors (dpeaa)DE-He213 Transcription factor domains (dpeaa)DE-He213 Directed evolution (dpeaa)DE-He213 Anthocyanin (dpeaa)DE-He213 Xie, Claire H. verfasserin aut Yuan, Ling verfasserin aut Enthalten in Planta Berlin : Springer, 1925 227(2007), 3 vom: 13. Dez., Seite 707-715 (DE-627)254639100 (DE-600)1463030-8 1432-2048 nnns volume:227 year:2007 number:3 day:13 month:12 pages:707-715 https://dx.doi.org/10.1007/s00425-007-0676-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2946 GBV_ILN_2949 GBV_ILN_2951 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4393 GBV_ILN_4700 42.38 ASE AR 227 2007 3 13 12 707-715 |
language |
English |
source |
Enthalten in Planta 227(2007), 3 vom: 13. Dez., Seite 707-715 volume:227 year:2007 number:3 day:13 month:12 pages:707-715 |
sourceStr |
Enthalten in Planta 227(2007), 3 vom: 13. Dez., Seite 707-715 volume:227 year:2007 number:3 day:13 month:12 pages:707-715 |
format_phy_str_mv |
Article |
institution |
findex.gbv.de |
topic_facet |
Basic helix–loop–helix transcription factors Transcription factor domains Directed evolution Anthocyanin |
dewey-raw |
580 |
isfreeaccess_bool |
false |
container_title |
Planta |
authorswithroles_txt_mv |
Pattanaik, Sitakanta @@aut@@ Xie, Claire H. @@aut@@ Yuan, Ling @@aut@@ |
publishDateDaySort_date |
2007-12-13T00:00:00Z |
hierarchy_top_id |
254639100 |
dewey-sort |
3580 |
id |
SPR005654572 |
language_de |
englisch |
fullrecord |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">SPR005654572</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230520001614.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">201002s2007 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/s00425-007-0676-y</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)SPR005654572</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(SPR)s00425-007-0676-y-e</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">580</subfield><subfield code="q">ASE</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">42.38</subfield><subfield code="2">bkl</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Pattanaik, Sitakanta</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="4"><subfield code="a">The interaction domains of the plant Myc-like bHLH transcription factors can regulate the transactivation strength</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2007</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract The N-terminal region of the plant Myc-like basic helix–loop–helix transcription factors (bHLH TFs) contains two domains. Approximately, 190 amino acids at the N-terminus comprise an interaction domain, a.k.a. Myb-interacting-region (MIR) for its primary function of interacting with Myb-like TFs. Following, the interaction domain is an activation (or acidic) domain responsible for transactivation. We have previously discovered that a lysine to methionine substitution (K157M) in the interaction domain of Myc-RP of Perilla frutescens leads to a 50-fold increase in transactivation activity. The result suggests that mutations in the interaction domain affect transactivation. The highly conserved nature of this lysine residue in many Myc-like bHLH TFs prompted us to explore the functional importance of this residue within the TF family and the influence of the interaction domain on the activation domain in transactivation. We found that the replacement of the equivalent lysine with methionine significantly affects the transactivation activities of two other Myc-RP homologues, Delila from snapdragon and Lc from maize. In addition to methionine, substitution with several other amino acids at this position has positive effects on transcriptional activity. A neighboring conserved alanine residue (A159 in Myc-RP, A161 in Delila and A172 in Lc) also affects transactivation. Substitution of this alanine residue to an aspartic acid abolished transactivation of both Myc-RP and Delila and severely reduced transactivation of Lc. Ectopic expression of a Myc-RP K157M mutant in transgenic tobacco resulted in increased anthocyanin accumulation compared to plants expressing the wild-type gene. Our study reveals the potential cooperation between functional domains of the bHLH TFs.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Basic helix–loop–helix transcription factors</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Transcription factor domains</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Directed evolution</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Anthocyanin</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Xie, Claire H.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Yuan, Ling</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Planta</subfield><subfield code="d">Berlin : Springer, 1925</subfield><subfield code="g">227(2007), 3 vom: 13. Dez., Seite 707-715</subfield><subfield code="w">(DE-627)254639100</subfield><subfield code="w">(DE-600)1463030-8</subfield><subfield code="x">1432-2048</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:227</subfield><subfield code="g">year:2007</subfield><subfield code="g">number:3</subfield><subfield code="g">day:13</subfield><subfield code="g">month:12</subfield><subfield code="g">pages:707-715</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://dx.doi.org/10.1007/s00425-007-0676-y</subfield><subfield code="z">lizenzpflichtig</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_SPRINGER</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-PHA</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_11</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_20</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_22</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_23</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_24</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_31</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_32</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_39</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_40</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_60</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_62</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_63</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_65</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_69</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_70</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_73</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_74</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_90</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_95</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_100</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_105</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_110</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_120</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_138</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_150</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_151</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_152</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_161</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_170</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_171</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_187</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_213</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_224</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_230</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_250</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_267</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_281</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_285</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_293</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_370</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_374</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_602</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_636</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_647</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_702</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2001</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2003</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2004</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2005</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2006</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2007</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2008</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2009</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2010</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2011</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2014</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2015</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2018</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2020</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2021</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2025</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2026</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2027</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2031</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2034</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2037</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2038</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2039</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2044</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2048</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2049</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2050</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2055</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2057</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2059</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2061</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2064</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2065</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2068</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2070</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2086</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2088</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2093</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2106</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2107</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2108</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2110</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2111</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2112</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2113</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2116</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2118</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2119</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2122</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2129</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2143</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2144</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2147</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2148</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2152</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2153</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2188</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2190</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2232</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2336</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2446</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2470</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2472</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2507</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2522</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2548</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2946</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2949</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2951</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4012</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4035</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4037</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4046</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4112</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4125</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4126</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4242</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4246</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4249</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4251</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4305</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4306</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4307</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4313</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4322</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4323</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4324</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4325</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4326</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4328</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4333</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4334</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4335</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4336</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4338</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4346</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4393</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4700</subfield></datafield><datafield tag="936" ind1="b" ind2="k"><subfield code="a">42.38</subfield><subfield code="q">ASE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">227</subfield><subfield code="j">2007</subfield><subfield code="e">3</subfield><subfield code="b">13</subfield><subfield code="c">12</subfield><subfield code="h">707-715</subfield></datafield></record></collection>
|
author |
Pattanaik, Sitakanta |
spellingShingle |
Pattanaik, Sitakanta ddc 580 bkl 42.38 misc Basic helix–loop–helix transcription factors misc Transcription factor domains misc Directed evolution misc Anthocyanin The interaction domains of the plant Myc-like bHLH transcription factors can regulate the transactivation strength |
authorStr |
Pattanaik, Sitakanta |
ppnlink_with_tag_str_mv |
@@773@@(DE-627)254639100 |
format |
electronic Article |
dewey-ones |
580 - Plants (Botany) |
delete_txt_mv |
keep |
author_role |
aut aut aut |
collection |
springer |
remote_str |
true |
illustrated |
Not Illustrated |
issn |
1432-2048 |
topic_title |
580 ASE 42.38 bkl The interaction domains of the plant Myc-like bHLH transcription factors can regulate the transactivation strength Basic helix–loop–helix transcription factors (dpeaa)DE-He213 Transcription factor domains (dpeaa)DE-He213 Directed evolution (dpeaa)DE-He213 Anthocyanin (dpeaa)DE-He213 |
topic |
ddc 580 bkl 42.38 misc Basic helix–loop–helix transcription factors misc Transcription factor domains misc Directed evolution misc Anthocyanin |
topic_unstemmed |
ddc 580 bkl 42.38 misc Basic helix–loop–helix transcription factors misc Transcription factor domains misc Directed evolution misc Anthocyanin |
topic_browse |
ddc 580 bkl 42.38 misc Basic helix–loop–helix transcription factors misc Transcription factor domains misc Directed evolution misc Anthocyanin |
format_facet |
Elektronische Aufsätze Aufsätze Elektronische Ressource |
format_main_str_mv |
Text Zeitschrift/Artikel |
carriertype_str_mv |
cr |
hierarchy_parent_title |
Planta |
hierarchy_parent_id |
254639100 |
dewey-tens |
580 - Plants (Botany) |
hierarchy_top_title |
Planta |
isfreeaccess_txt |
false |
familylinks_str_mv |
(DE-627)254639100 (DE-600)1463030-8 |
title |
The interaction domains of the plant Myc-like bHLH transcription factors can regulate the transactivation strength |
ctrlnum |
(DE-627)SPR005654572 (SPR)s00425-007-0676-y-e |
title_full |
The interaction domains of the plant Myc-like bHLH transcription factors can regulate the transactivation strength |
author_sort |
Pattanaik, Sitakanta |
journal |
Planta |
journalStr |
Planta |
lang_code |
eng |
isOA_bool |
false |
dewey-hundreds |
500 - Science |
recordtype |
marc |
publishDateSort |
2007 |
contenttype_str_mv |
txt |
container_start_page |
707 |
author_browse |
Pattanaik, Sitakanta Xie, Claire H. Yuan, Ling |
container_volume |
227 |
class |
580 ASE 42.38 bkl |
format_se |
Elektronische Aufsätze |
author-letter |
Pattanaik, Sitakanta |
doi_str_mv |
10.1007/s00425-007-0676-y |
dewey-full |
580 |
author2-role |
verfasserin |
title_sort |
interaction domains of the plant myc-like bhlh transcription factors can regulate the transactivation strength |
title_auth |
The interaction domains of the plant Myc-like bHLH transcription factors can regulate the transactivation strength |
abstract |
Abstract The N-terminal region of the plant Myc-like basic helix–loop–helix transcription factors (bHLH TFs) contains two domains. Approximately, 190 amino acids at the N-terminus comprise an interaction domain, a.k.a. Myb-interacting-region (MIR) for its primary function of interacting with Myb-like TFs. Following, the interaction domain is an activation (or acidic) domain responsible for transactivation. We have previously discovered that a lysine to methionine substitution (K157M) in the interaction domain of Myc-RP of Perilla frutescens leads to a 50-fold increase in transactivation activity. The result suggests that mutations in the interaction domain affect transactivation. The highly conserved nature of this lysine residue in many Myc-like bHLH TFs prompted us to explore the functional importance of this residue within the TF family and the influence of the interaction domain on the activation domain in transactivation. We found that the replacement of the equivalent lysine with methionine significantly affects the transactivation activities of two other Myc-RP homologues, Delila from snapdragon and Lc from maize. In addition to methionine, substitution with several other amino acids at this position has positive effects on transcriptional activity. A neighboring conserved alanine residue (A159 in Myc-RP, A161 in Delila and A172 in Lc) also affects transactivation. Substitution of this alanine residue to an aspartic acid abolished transactivation of both Myc-RP and Delila and severely reduced transactivation of Lc. Ectopic expression of a Myc-RP K157M mutant in transgenic tobacco resulted in increased anthocyanin accumulation compared to plants expressing the wild-type gene. Our study reveals the potential cooperation between functional domains of the bHLH TFs. |
abstractGer |
Abstract The N-terminal region of the plant Myc-like basic helix–loop–helix transcription factors (bHLH TFs) contains two domains. Approximately, 190 amino acids at the N-terminus comprise an interaction domain, a.k.a. Myb-interacting-region (MIR) for its primary function of interacting with Myb-like TFs. Following, the interaction domain is an activation (or acidic) domain responsible for transactivation. We have previously discovered that a lysine to methionine substitution (K157M) in the interaction domain of Myc-RP of Perilla frutescens leads to a 50-fold increase in transactivation activity. The result suggests that mutations in the interaction domain affect transactivation. The highly conserved nature of this lysine residue in many Myc-like bHLH TFs prompted us to explore the functional importance of this residue within the TF family and the influence of the interaction domain on the activation domain in transactivation. We found that the replacement of the equivalent lysine with methionine significantly affects the transactivation activities of two other Myc-RP homologues, Delila from snapdragon and Lc from maize. In addition to methionine, substitution with several other amino acids at this position has positive effects on transcriptional activity. A neighboring conserved alanine residue (A159 in Myc-RP, A161 in Delila and A172 in Lc) also affects transactivation. Substitution of this alanine residue to an aspartic acid abolished transactivation of both Myc-RP and Delila and severely reduced transactivation of Lc. Ectopic expression of a Myc-RP K157M mutant in transgenic tobacco resulted in increased anthocyanin accumulation compared to plants expressing the wild-type gene. Our study reveals the potential cooperation between functional domains of the bHLH TFs. |
abstract_unstemmed |
Abstract The N-terminal region of the plant Myc-like basic helix–loop–helix transcription factors (bHLH TFs) contains two domains. Approximately, 190 amino acids at the N-terminus comprise an interaction domain, a.k.a. Myb-interacting-region (MIR) for its primary function of interacting with Myb-like TFs. Following, the interaction domain is an activation (or acidic) domain responsible for transactivation. We have previously discovered that a lysine to methionine substitution (K157M) in the interaction domain of Myc-RP of Perilla frutescens leads to a 50-fold increase in transactivation activity. The result suggests that mutations in the interaction domain affect transactivation. The highly conserved nature of this lysine residue in many Myc-like bHLH TFs prompted us to explore the functional importance of this residue within the TF family and the influence of the interaction domain on the activation domain in transactivation. We found that the replacement of the equivalent lysine with methionine significantly affects the transactivation activities of two other Myc-RP homologues, Delila from snapdragon and Lc from maize. In addition to methionine, substitution with several other amino acids at this position has positive effects on transcriptional activity. A neighboring conserved alanine residue (A159 in Myc-RP, A161 in Delila and A172 in Lc) also affects transactivation. Substitution of this alanine residue to an aspartic acid abolished transactivation of both Myc-RP and Delila and severely reduced transactivation of Lc. Ectopic expression of a Myc-RP K157M mutant in transgenic tobacco resulted in increased anthocyanin accumulation compared to plants expressing the wild-type gene. Our study reveals the potential cooperation between functional domains of the bHLH TFs. |
collection_details |
GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2946 GBV_ILN_2949 GBV_ILN_2951 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4393 GBV_ILN_4700 |
container_issue |
3 |
title_short |
The interaction domains of the plant Myc-like bHLH transcription factors can regulate the transactivation strength |
url |
https://dx.doi.org/10.1007/s00425-007-0676-y |
remote_bool |
true |
author2 |
Xie, Claire H. Yuan, Ling |
author2Str |
Xie, Claire H. Yuan, Ling |
ppnlink |
254639100 |
mediatype_str_mv |
c |
isOA_txt |
false |
hochschulschrift_bool |
false |
doi_str |
10.1007/s00425-007-0676-y |
up_date |
2024-07-03T17:48:30.007Z |
_version_ |
1803581034339500032 |
fullrecord_marcxml |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">SPR005654572</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230520001614.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">201002s2007 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/s00425-007-0676-y</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)SPR005654572</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(SPR)s00425-007-0676-y-e</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">580</subfield><subfield code="q">ASE</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">42.38</subfield><subfield code="2">bkl</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Pattanaik, Sitakanta</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="4"><subfield code="a">The interaction domains of the plant Myc-like bHLH transcription factors can regulate the transactivation strength</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2007</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract The N-terminal region of the plant Myc-like basic helix–loop–helix transcription factors (bHLH TFs) contains two domains. Approximately, 190 amino acids at the N-terminus comprise an interaction domain, a.k.a. Myb-interacting-region (MIR) for its primary function of interacting with Myb-like TFs. Following, the interaction domain is an activation (or acidic) domain responsible for transactivation. We have previously discovered that a lysine to methionine substitution (K157M) in the interaction domain of Myc-RP of Perilla frutescens leads to a 50-fold increase in transactivation activity. The result suggests that mutations in the interaction domain affect transactivation. The highly conserved nature of this lysine residue in many Myc-like bHLH TFs prompted us to explore the functional importance of this residue within the TF family and the influence of the interaction domain on the activation domain in transactivation. We found that the replacement of the equivalent lysine with methionine significantly affects the transactivation activities of two other Myc-RP homologues, Delila from snapdragon and Lc from maize. In addition to methionine, substitution with several other amino acids at this position has positive effects on transcriptional activity. A neighboring conserved alanine residue (A159 in Myc-RP, A161 in Delila and A172 in Lc) also affects transactivation. Substitution of this alanine residue to an aspartic acid abolished transactivation of both Myc-RP and Delila and severely reduced transactivation of Lc. Ectopic expression of a Myc-RP K157M mutant in transgenic tobacco resulted in increased anthocyanin accumulation compared to plants expressing the wild-type gene. Our study reveals the potential cooperation between functional domains of the bHLH TFs.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Basic helix–loop–helix transcription factors</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Transcription factor domains</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Directed evolution</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Anthocyanin</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Xie, Claire H.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Yuan, Ling</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Planta</subfield><subfield code="d">Berlin : Springer, 1925</subfield><subfield code="g">227(2007), 3 vom: 13. Dez., Seite 707-715</subfield><subfield code="w">(DE-627)254639100</subfield><subfield code="w">(DE-600)1463030-8</subfield><subfield code="x">1432-2048</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:227</subfield><subfield code="g">year:2007</subfield><subfield code="g">number:3</subfield><subfield code="g">day:13</subfield><subfield code="g">month:12</subfield><subfield code="g">pages:707-715</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://dx.doi.org/10.1007/s00425-007-0676-y</subfield><subfield code="z">lizenzpflichtig</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_SPRINGER</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-PHA</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_11</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_20</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_22</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_23</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_24</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_31</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_32</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_39</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_40</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_60</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_62</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_63</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_65</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_69</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_70</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_73</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_74</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_90</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_95</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_100</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_105</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_110</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_120</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_138</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_150</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_151</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_152</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_161</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_170</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_171</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_187</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_213</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_224</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_230</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_250</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_267</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_281</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_285</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_293</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_370</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_374</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_602</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_636</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_647</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_702</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2001</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2003</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2004</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2005</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2006</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2007</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2008</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2009</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2010</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2011</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2014</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2015</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2018</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2020</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2021</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2025</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2026</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2027</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2031</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2034</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2037</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2038</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2039</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2044</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2048</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2049</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2050</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2055</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2057</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2059</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2061</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2064</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2065</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2068</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2070</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2086</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2088</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2093</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2106</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2107</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2108</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2110</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2111</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2112</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2113</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2116</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2118</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2119</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2122</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2129</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2143</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2144</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2147</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2148</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2152</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2153</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2188</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2190</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2232</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2336</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2446</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2470</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2472</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2507</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2522</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2548</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2946</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2949</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2951</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4012</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4035</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4037</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4046</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4112</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4125</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4126</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4242</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4246</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4249</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4251</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4305</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4306</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4307</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4313</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4322</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4323</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4324</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4325</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4326</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4328</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4333</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4334</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4335</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4336</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4338</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4346</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4393</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4700</subfield></datafield><datafield tag="936" ind1="b" ind2="k"><subfield code="a">42.38</subfield><subfield code="q">ASE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">227</subfield><subfield code="j">2007</subfield><subfield code="e">3</subfield><subfield code="b">13</subfield><subfield code="c">12</subfield><subfield code="h">707-715</subfield></datafield></record></collection>
|
score |
7.3981857 |